ライフサイエンスコーパス: unspliced

1 d by ICP27 transactivation was predominantly unspliced.

2 iptional analysis of this mutant showed that unspliced 19S RNA was not transported and remained withi

3 ns: CXCR4-A and CXCR4-B, corresponding to an unspliced and a spliced mRNA, respectively.

4 oximately half of the RNA transcripts remain unspliced and either are used to encode Gag and Gag-Pol

5 In addition, we found ~200 long, unspliced and exosome-sensitive antisense RNAs that aris

6 ex with a 351 nucleotide sequence present in unspliced and incompletely spliced human immunodeficienc

7 for efficient cytoplasmic expression of the unspliced and incompletely spliced viral mRNA transcript

8 to induce the cytoplasmic expression of the unspliced and incompletely spliced viral RNAs encoding t

9 approximately half of the viral RNA remains unspliced and is used as genomic RNA and as mRNA for the

10 Both unspliced and multiply spliced forms were found.

11 Patient-matched PCR for unspliced and multiply spliced viral RNAs combined with

12 Here, we show that unspliced and partially spliced forms of the MATa1 mRNA

13 ntial for the nucleocytoplasmic transport of unspliced and partially spliced HIV mRNAs containing the

14 elements (RREs) is required for transport of unspliced and partially spliced human immunodeficiency v

15 IV-1 Rev protein activates nuclear export of unspliced and partially spliced viral RNA transcripts, w

16 complex signals nucleocytoplasmic export of unspliced and partially spliced viral RNA.

17 ponse element (RRE) is a 351-base element in unspliced and partially spliced viral RNA; binding of th

18 cialized nuclear export pathway to transport unspliced and partially spliced viral transcripts to the

19 The UL37 promoter drives production of an unspliced and several alternatively spliced RNAs.

20 (RRE) is essential for the nuclear export of unspliced and singly spliced human immunodeficiency viru

21 dramatically decreased accumulation of HIV-1 unspliced and singly spliced RNAs and altered splice sit

22 Nuclear export of unspliced and singly spliced viral mRNA is a critical st

23 HIV replication requires nuclear export of unspliced and singly spliced viral transcripts.

24 therefore indispensable for the transport of unspliced and singly spliced viral transcripts.

25 plication by mediating the nuclear export of unspliced and singly-spliced viral mRNAs.

26 A single transcript in its unspliced and spliced forms directs the synthesis of all

27 he rapid and transient induction of both the unspliced and spliced forms of the UPR gene bZIP60.

28 died the in vitro dimerization properties of unspliced and spliced HIV-2 RNA.

29 xpressed in skeletal muscle and exists as in unspliced and spliced isoforms, and its 5' end overlaps

30 These unspliced and spliced transcripts and putative proteins

31 Consistent with this prediction, both unspliced and spliced UL29/28 transcript was present in

32 Analysis of the levels of unspliced and spliced viral RNA produced by the parental

33 JSRV encodes unspliced and spliced viral RNAs, among which unspliced

34 in B2, OcaB (BOB1, Pou2af1), and XBP1 mRNAs, unspliced and spliced, are severely reduced in ELL2-defi

35 HBV DNA quantification with S (unspliced) and X (total DNA) regions provided different

36 aining the proper equilibrium among spliced, unspliced, and partially spliced isoforms is essential f

37 rrence of variants and molecules that remain unspliced at nearby exon-intron boundaries.

38 Rather, it retains the unspliced beta-globin mRNA in the nucleus.

39 riments also showed that p68 interacted with unspliced but not spliced mRNA in vivo.

40 gle intron; in maize leaves both spliced and unspliced Bz2 transcripts are usually present and are pr

41 safe but did not increase plasma viremia or unspliced CA-RNA despite pharmacodynamic effects on CD4

42 es a significant increase in cell-associated unspliced (CA-US) HIV-1 RNA from CD4(+) T cells.

43 r levels of HIV DNA (P< 0.001) and increased unspliced cellular HIV RNA transcription (P= 0.010).

44 een levels of virions in the supernatant and unspliced cellular HIV-1 RNA following anti-CD3/CD28 tre

45 ic acid (SAHA; vorinostat) show increases in unspliced cellular HIV-1 RNA levels in resting CD4(+) T

46 ns, and the exonic structures of spliced and unspliced coding and noncoding RNAs.

47 ion is attenuated, Gag binding promoted, and unspliced dimeric genomes selected, by the RNA conformer

48 ments that direct selective packaging of the unspliced, dimeric viral RNA into assembling particles.

49 omitant with an increase in the abundance of unspliced early transcripts.

50 ture at 5' splice sites that correlates with unspliced events.

51 ich exists in the transcriptionally inactive unspliced form [XBP1(U)] and the spliced active form [XB

52 ot silent, are capable of transcribing novel unspliced forms of HIV-RNA transcripts with competent op

53 Herein we identified unspliced forms of LDH and ENO transcripts produced duri

54 lated by the balance between its spliced and unspliced forms.

55 ization capture strategy for purification of unspliced full-length HIV RNA-protein complexes preserve

56 er and results in increased transcription of unspliced gag and spliced nef viral RNA.

57 these mutants, we quantitated the levels of unspliced gag and spliced pol mRNAs using a real-time PC

58 educed as much as 30-fold, whereas levels of unspliced gag RNA were not affected.

59 protein production and the nuclear export of unspliced gag-pol RNA.

60 SRp40 increased nuclear localization of the unspliced Gag/Pol mRNA, while the same factors increased

61 of HSV-2 ICP34.5 can be translated from the unspliced gamma34.5 mRNA.

62 RP2 causes a defective spliceosome to retain unspliced gene transcripts in the nuclei of human cells.

63 press Gag and Pol proteins by translation of unspliced genome-length viral RNA.

64 of spliced env mRNA and highly enriched for unspliced genome.

65 green fluorescent protein (EGFP) within its unspliced genomic context.

66 luding alternative splicing and packaging of unspliced genomic RNA into virions.

67 Full-length unspliced genomic RNA plays critical roles in HIV replic

68 cy virus type 1, synthesize Gag and Pol from unspliced genomic RNA.

69 A piRNAs are almost exclusively derived from unspliced genomic transcripts and are strongly sense-str

70 ete foci of higher-order oligomers, to which unspliced HAC1 mRNA is recruited by means of a conserved

71 ges are detected in the levels of spliced or unspliced HAC1 mRNA or in the stability of Hac1p.

72 sence of ER stress, ribosomes are stalled on unspliced HAC1 mRNA.

73 tion represses translation initiation on the unspliced HAC1 mRNA.

74 ction site that derepress translation of the unspliced HAC1 mRNA.

75 Whereas in yeast translation of unspliced Hac1p is blocked, mammalian Xbp-1u is synthesi

76 elimination of unspliced Xbp-1 (Xbp-1u) and unspliced Hac1p, respectively.

77 An unspliced HBV genome was dominant, but 100% of strains w

78 i (Type 2 proviruses), nuclear export of the unspliced HERV-K mRNA appears to be mediated by a cis-ac

79 Platelets contain unspliced heteronuclear IL-1beta RNA, which is rapidly s

80 ram resulted in increases in cell-associated unspliced HIV RNA at all doses, consistent with activati

81 e median maximum increase in cell-associated unspliced HIV RNA during panobinostat treatment was 3.5-

82 estimated fold increases in cell-associated unspliced HIV RNA from baseline were 1.7 (95% CI 1.3-2.2

83 imary endpoint was change in cell-associated unspliced HIV RNA in CD4 cells.

84 t 10.6 latency cellular models and increased unspliced HIV RNA in resting CD4+ T cells isolated from

85 ne proteins were identified to interact with unspliced HIV RNA including Rev and Gag/Gag-Pol, 24 host

86 The level of cell-associated unspliced HIV RNA increased significantly at all timepoi

87 HIV DNA, 2-LTR circles, and cell-associated unspliced HIV RNA were quantified.

88 Cell-associated integrated HIV DNA, unspliced HIV RNA, and chemokine messenger RNA were quan

89 was change from baseline of cell-associated unspliced HIV RNA.

90 y attenuates the translational output of the unspliced HIV-1 gag mRNA, and possibly all HIV-1 transcr

91 that nuclear Naf1 promoted nuclear export of unspliced HIV-1 gag mRNA, leading to increased Gag produ

92 er to allow the accumulation of vif mRNA and unspliced HIV-1 mRNA, compatible with optimal virus repl

93 n(s) that directs cytoplasmic utilization of unspliced HIV-1 reporter RNA.

94 Only very low levels of unspliced HIV-1 RNA ( approximately 50 copies/10(6) rest

95 HIV-1 RNA assay, HIV-1 DNA, cell-associated unspliced HIV-1 RNA (CA-RNA), acetylation of histone H3-

96 t (Psi) within the 5' untranslated region of unspliced HIV-1 RNA genome.

97 steady-state levels of multiply spliced and unspliced HIV-1 RNA prior to cellular activation suggest

98 Nuclear export of partially spliced or unspliced HIV-1 RNA transcripts requires binding of the

99 peckles, a structure implicated in retaining unspliced HIV-1 transcripts for either Rev-mediated nucl

100 ve" proviruses capable of transcribing novel unspliced HIV-RNA (usHIV-RNA) species in patients at all

101 e more indicative of antigen expression than unspliced HIV-RNAs and may help to explain the limited a

102 Although unspliced host messenger RNAs are rarely exported from t

103 responsive element-independent expression of unspliced human immunodeficiency virus type 1 (HIV-1) ga

104 Full-length unspliced human immunodeficiency virus type 1 (HIV-1) RN

105 ICP34.5beta is translated from unspliced ICP34.5 mRNA, with the retained intron introdu

106 ed population and a population consisting of unspliced IEX-1 RNA.

107 rther found both Xist129 and XistCAS RNA are unspliced in Mus musculus 129SvJ/Mus castaneous (CAS) hy

108 We found that Xist RNA is unspliced in naive embryonic stem (ES) cells.

109 es of the presence of either a spliced or an unspliced intron in a rRNA for ribosome assembly and pac

110 We have previously demonstrated that unspliced intron-containing CTE RNA is efficiently expor

111 These aberrations accompany increased unspliced (intron-retained) and decreased spliced mRNA o

112 ent (CTE), an element that enables export of unspliced, intron-containing mRNA.

113 ated nascent pre-mRNA (CA-RNA) contains many unspliced introns and m(6)A in exons but very rarely in

114 their genomic and mRNAs contain one or more unspliced introns.

115 1ot1 is transcribed by RNA polymerase II, is unspliced, is relatively stable and is localised in the

116 e UPR is inactive, HAC1 mRNA is stored as an unspliced isoform in the cytoplasm and no Hac1 protein i

117 with Rej deleted showed normal transport of unspliced JSRV RNA to the cytoplasm; however, in 293T ce

118 The unspliced kay(sro) transcript has a lower abundance than

119 s) 71, 72 and 73 proteins as well as a novel unspliced KSHV mRNA that encodes only ORF72 and ORF71.

120 When relative amounts of the spliced and unspliced LAT within the brain, liver, kidney, spinal co

121 expression of both the spliced (active) and unspliced (latent) forms of XBP1 mRNAs.

122 slational efficiency compared to that of the unspliced leader.

123 Unspliced LEF1 NAT interacts with LEF1 promoter and faci

124 ts influence the position of genes and their unspliced length.

125 t machinery or how the intron-containing but unspliced M1 mRNA bypasses the normal quality-control ch

126 KL is produced by cytoplasmic translation of unspliced M1 mRNA initiating at CUG codons within the +1

127 The unspliced M1 mRNA is translated into the matrix M1 prote

128 The unspliced M1 mRNA is translated into the matrix M1 prote

129 n, we found that cytoplasmic accumulation of unspliced M1 mRNA was inefficient in the absence of NS1,

130 vide a means of estimating the proportion of unspliced MoMLV RNA that serves as genomic RNA.

131 two proteins: the M1 protein translated from unspliced mRNA and the M2 protein produced by mRNA splic

132 comparison, the protein translated from the unspliced mRNA contains a transmembrane domain, localize

133 Segment 7 produces two major transcripts: an unspliced mRNA that encodes the M1 matrix protein and a

134 cripts and effect export from the nucleus of unspliced mRNA, thereby allowing the synthesis of struct

135 This isoform is shown to represent intron 0 unspliced mRNA, whereas the smaller transcript represent

136 runcated D1 (exons 1-4) polypeptide from the unspliced mRNA.

137 fficient polyadenylation of both spliced and unspliced mRNAs (encoding a putative 10-kDa protein, ana

138 proviruses or reporter gene expression from unspliced mRNAs and allowed detection of a 33-kDa protei

139 Nevertheless, we show that unspliced mRNAs are mostly prevented from reaching the n

140 ning complexes that contain both spliced and unspliced mRNAs of housekeeping genes.

141 anslated several times more efficiently than unspliced mRNAs that have the same sequence but lack EJC

142 oviral replication requires both spliced and unspliced mRNAs.

143 ntially higher levels from spliced than from unspliced mRNAs.

144 pies of Vhs from infecting virions than were unspliced mRNAs.

145 However, unspliced nascent transcripts were not detected.

146 ing leads to a more dramatic accumulation of unspliced nascent transcripts.

147 scription, as assessed by the measurement of unspliced, nascent, heterogeneous nuclear RNA, and treat

148 of LEF1 NAT in trans prevents the action of unspliced NAT by competing for interaction with the prom

149 Contrarily to the spliced NAT, this unspliced NAT down-regulates the main LEF1 promoter acti

150 the mRNA encoding Orb2A protein exists in an unspliced non-protein-coding form.

151 extent, the transcripts produced tend to be unspliced, non-polyadenylated and expressed at levels 10

152 NS1-ARF2(1-8) is presented from unspliced NS mRNA, likely from downstream initiation on

153 of miR-155 is likely a transient spliced or unspliced nuclear BIC transcript rather than accumulated

154 transcripts is exported from the nucleus in unspliced or incompletely spliced forms to serve as temp

155 ionally and allows the cytoplasmic export of unspliced or incompletely spliced viral mRNAs carrying g

156 ncoded by P9-generated mRNAs that are either unspliced or spliced within the rep gene region, respect

157 We posit that pasilla-mediated processing of unspliced Orb2A mRNA integrates experience and internal

158 We have mapped the 5' and 3' ends of the unspliced Orf49 transcript, which encodes a 30-kDa prote

159 of Rep 1 was initiated from the first AUG in unspliced P9-generated mRNA; however, this AUG was bypas

160 M-1 is also used to export Rev/RRE-dependent unspliced/ partially spliced HIV-1 RNAs.

161 TMG capping of unspliced/partially spliced HIV-1 RNAs represents a new

162 o1 mutants, ycf3-intron 2 remains completely unspliced, petD intron splicing is strongly reduced, and

163 mRNA splicing, resulting in accumulation of unspliced pre-mRNA and alternatively spliced mRNA.

164 ination codons, but the role of NMD on yeast unspliced pre-mRNA degradation is controversial.

165 t associates with spliced mRNA, but not with unspliced pre-mRNA in vitro.

166 ignals, surprisingly, apparently full-length unspliced pre-mRNA persisted for several hours in both c

167 cised, lariat form of the intron, as well as unspliced pre-mRNA, suggesting a role for Cwc23 in splic

168 4 and spt5 mutations lead to accumulation of unspliced pre-mRNA.

169 nd RPL18A transcripts trigger degradation of unspliced pre-mRNAs and lariat introns and can control t

170 decline of mRNAs, a concomitant increase of unspliced pre-mRNAs and the disappearance of the trans-s

171 wn in HeLa cells, and identified accumulated unspliced pre-mRNAs by genomic tiling microarrays.

172 MP components led to further accumulation of unspliced pre-mRNAs even in a yeast strain defective in

173 gs with Mlp1, both Esc1 and Ulp1 help retain unspliced pre-mRNAs in the nucleus.

174 scripts and promote the nuclear retention of unspliced pre-mRNAs in yeast.

175 Unspliced pre-mRNAs were also identified as targets for

176 nd nucleolar RNAs and in the surveillance of unspliced pre-mRNAs.

177 fic cell death and a significant increase in unspliced pre-mRNAs.

178 (iii) The unspliced pre-RNA form containing the IEX-1 intron seque

179 remature processing of the 5' and 3' ends of unspliced pre-tRNA.

180 epletion of mature tRNAs and accumulation of unspliced pre-tRNAs.

181 ng a 1.2-kbp RT-PCR product) arising from an unspliced precursor likely contributed, albeit to a less

182 yssi can be overexpressed and purified as an unspliced precursor, which allows for a detailed in vitr

183 essed in Escherichia coli and purified as an unspliced precursor.

184 In the absence of functional NMD, unspliced precursors accumulate in the cytoplasm, possib

185 e site mutation in RPS10B, and limits RPS29B unspliced precursors accumulation during amino acid star

186 embrane protein 2A (LMP2A) transcripts whose unspliced precursors cross joined TRs.

187 of unspliced RPS22B pre-mRNAs, degrades most unspliced precursors generated by a 5' splice site mutat

188 Significantly, selective piRNA processing of unspliced proviral transcripts is conserved from insects

189 of 8-mers in internal noncoding exons versus unspliced pseudo exons and 5' untranslated regions (5' u

190 NMD is capable of targeting both spliced and unspliced PTC-containing mRNAs.

191 ar factor Upf1 are required for the decay of unspliced, PTC-bearing RSV RNA by the NMD pathway.

192 were transfected with plasmids harboring an unspliced renilla luciferase (RLuc) reporter mRNA or RLu

193 ts, NXF1:NXT1 also facilitates the export of unspliced retroviral genomic RNA from simple type-D retr

194 Despite the presence of a 5- to 7-kb 3' UTR, unspliced retroviral RNA escapes this degradation.

195 ially tethering various EJC components to an unspliced RLuc transcript.

196 'UTR conformation on ribosome loading to HIV unspliced RNA and rate of Gag polypeptide synthesis was

197 ites, and on structural interactions between unspliced RNA and small nuclear RNAs in spliceosomal int

198 wo distinct signals derived from spliced and unspliced RNA are measured, providing the basis for a ro

199 WT1(+KTS) expression increases the levels of unspliced RNA containing a CTE and specifically promotes

200 G8 also enhanced polyribosome association of unspliced RNA containing a CTE.

201 9G8 was shown to enhance expression of unspliced RNA containing either the Mason-Pfizer monkey

202 oteins which favors production of the UL37x1 unspliced RNA during HCMV infection at the posttranscrip

203 nspliced and spliced viral RNAs, among which unspliced RNA encodes Gag and Pol proteins and a singly

204 arcoma virus (RSV) requires large amounts of unspliced RNA for replication.

205 virus selectively packages two copies of its unspliced RNA genome, both of which are utilized for str

206 jRE did not affect the levels of cytoplasmic unspliced RNA in 293 cells, although the unspliced RNA s

207 d nuclear export/accumulation of cytoplasmic unspliced RNA in 293T cells, similarly to other complex

208 ar RNA revealed a major fraction of nascent, unspliced RNA in contrast to results obtained from purif

209 integrated DNA as well as multi-spliced and unspliced RNA in divergent proportions.

210 rates that Tap and NXT efficiently shift the unspliced RNA into polyribosomal fractions.

211 The UL37 exon 1 (UL37x1) unspliced RNA is abundant from IE to late times of HCMV

212 Cell-associated HIV unspliced RNA is an important marker of the viral reserv

213 The unspliced RNA molecules are selected for encapsidation f

214 own of MCM7 or SF3B3 significantly increased unspliced RNA of epidermal growth factor receptor, plate

215 Production of the UL37x1 unspliced RNA requires polyadenylation (PA) at nucleotid

216 ere organization, spindle pole assembly, and unspliced RNA retention.

217 mic unspliced RNA in 293 cells, although the unspliced RNA showed partial degradation, perhaps due to

218 egulated RNA processing with accumulation of unspliced RNA species in AD, including myc box-dependent

219 infrequently detected in gut, and ratios of unspliced RNA to DNA were lower in the colon and rectum

220 e cells were not dormant but were generating unspliced RNA transcripts before treatment was interrupt

221 ytoplasmic accumulations of both spliced and unspliced RNA transcripts of XMRV and MLV, resulting in

222 leocytoplasmic transport of both spliced and unspliced RNA transcripts, and RNA export mechanisms of

223 mportantly, lower viral transcription (HIV-1 unspliced RNA) and enhanced immune preservation (CD4/CD8

224 le-cell visualization of HIV (a) spliced and unspliced RNA, (b) cytoplasmic and nuclear DNA, and (c)

225 , Rev and Tat spliced RNAs exceeded those of unspliced RNA.

226 ions between cellular proteins and the viral unspliced RNA.

227 , stalled spliceosomes are disassembled, and unspliced RNAs are released.

228 They also use unspliced RNAs as mRNAs to produce the gag and pol gene

229 Yeast prp2 mutants accumulate unspliced RNAs from the vast majority of intron-containi

230 Retroviruses package full-length, unspliced RNAs into progeny virions as dimerized RNA gen

231 n of gene products from both the spliced and unspliced RNAs is essential, as cells expressing only on

232 Retroviral genomes consist of two unspliced RNAs linked noncovalently in a dimer.

233 The unspliced RNAs were retained in the nucleus, and block o

234 ctional, exosome-sensitive, relatively short unspliced RNAs, the generation of which is strongly rela

235 trategy for correctly sorting spliced versus unspliced RNAs.

236 ntron splicing regulation in vivo, levels of unspliced rnp-4f mRNA in dADAR mutant were compared to w

237 The results show that during embryogenesis unspliced rnp-4f mRNA levels fall by up to 85% in the mu

238 nt RNase III-mediated nuclear degradation of unspliced RPS22B pre-mRNAs, degrades most unspliced prec

239 fca mutations increased levels of unspliced sense FLC transcript, altered processing of an

240 SIV inoculation and quantified the levels of unspliced SIV RNA and spliced SIV RNA in tissue lysates

241 P-1 species and simultaneously stabilize the unspliced species that acts as a dominant negative.

242 If this intron remains unspliced, the resulting E6E7 mRNA expresses oncogenic E

243 Consistent with this finding, the ratios of unspliced to spliced HIV-1 mRNAs in mouse cells expressi

244 of splicing as the efficiency of converting unspliced to spliced RNA and show that it is highest for

245 osB pre-mRNA is regulated by the quantity of unspliced transcript available to the splicing machinery

246 Of these, 13 were spliced; a single unspliced transcript putatively encoded NS1.

247 terminal form is translated from the primary unspliced transcript to a stop codon within the intron u

248 eviously thought on the degradation of yeast-unspliced transcripts and plays an important role in dis

249 a protein was never detected indicating that unspliced transcripts are likely to be non-coding.

250 at PRGs generates low levels of full-length unspliced transcripts but fails to make mature, protein-

251 maretroviruses produce spliced Env mRNAs and unspliced transcripts encoding Gag, Pol, and the viral g

252 siRNAs interfere with the processing of the unspliced transcripts for the gp41 gene, tat, rev, and n

253 human PRP2/DHX16 results in accumulation of unspliced transcripts, similar to the outcome in yeast p

254 odel that they form cotranscriptionally from unspliced transcripts.

255 ferent fluorescent proteins from spliced and unspliced transcripts.

256 ch is transcript poor, containing only three unspliced transcripts.

257 d in degradation of cytoplasmic viral and/or unspliced transcripts.

258 and five nucleoporins cause accumulation of unspliced tRNA, a hallmark of defective tRNA nuclear exp

259 membrane proteins also cause accumulation of unspliced tRNA, likely due to defective splicing on mito

260 2 leads to rapid accumulation of end-matured unspliced tRNAs in the nucleus.

261 that could be produced by translation of an unspliced UL29/28 transcript.

262 The ratio of spliced to unspliced UL37 transcripts also changed.

263 LTR) circles, and multiply spliced (ms-) and unspliced (us-) HIV-1 RNA concentrations were measured a

264 Both spliced and unspliced variants of the new imprinted sense transcript

265 e resultant accumulation of both spliced and unspliced versions of some mRNAs in the cytoplasm.

266 ype 1 Gag precursor specifically selects the unspliced viral genomic RNA (gRNA) from the bulk of cell

267 required for export of partially spliced and unspliced viral mRNA from nuclei of infected cells, and

268 ced viral mRNA and decreased accumulation of unspliced viral mRNA, resulting in decreased cell-associ

269 ction of Rej is to facilitate translation of unspliced viral mRNA.

270 ailure to export RRE-containing CAT mRNA and unspliced viral mRNAs to the cytoplasm, confirming that

271 ement (RRE) RNA to mediate nuclear export of unspliced viral mRNAs.

272 n 293T cells Rej modestly enhanced export of unspliced viral RNA (2.8-fold).

273 Rem is required for nuclear export of unspliced viral RNA and efficient expression of viral pr

274 actions between the dimeric 5'-leader of the unspliced viral RNA and the nucleocapsid (NC) domains of

275 , and indicates that cells containing solely unspliced viral RNA are a good marker for viral latency.

276 uclear export or accumulation of cytoplasmic unspliced viral RNA in 293T cells but not in most other

277 HIV-1 Gag selects and packages a dimeric, unspliced viral RNA in the context of a large excess of

278 ants also showed deficits in accumulation of unspliced viral RNA in the cytoplasm.

279 virus type 1 (HIV-1) proviruses that express unspliced viral RNA in vivo or about the levels of HIV R

280 This results in a decrease of unspliced viral RNA levels and an approximately 10-fold

281 For some retroviruses, transport of unspliced viral RNA to the cytoplasm is mediated by smal

282 veral orders of magnitude less abundant than unspliced viral RNA, was slightly enriched relative to a

283 not have consistent effects on the amount of unspliced viral RNA, whereas the amount of cell-associat

284 essary for the synthesis of Gag protein from unspliced viral RNA.

285 nsible for synthesis of Gag polyprotein from unspliced viral RNA.

286 ation codon that prevents degradation of the unspliced viral RNA.

287 orted from the nucleus, MLV actively exports unspliced viral RNAs to the cytoplasm.

288 ed in the nuclear export of both spliced and unspliced viral RNAs, and, finally, (iii) depletion of N

289 ve special relevance during the formation of unspliced viral transcripts (p < 0.0005).

290 Rev response element in stem IIB located on unspliced viral transcripts and subsequently oligomerize

291 ted enhancement of the cytoplasmic levels of unspliced viral transcripts.

292 in mRNA was significantly increased in NASH, unspliced X-box protein-1 (XBP-1) protein did not increa

293 e the characteristic of rapid elimination of unspliced Xbp-1 (Xbp-1u) and unspliced Hac1p, respective

294 Transcription of unspliced XBP-1 mRNA is up-regulated by IL-2 signals, wh

295 teraction through C-terminal domain with the unspliced XBP1 and the inositol requiring enzyme 1 alpha

296 damental signaling activities of spliced and unspliced XBP1 in breast cancer, establish NF-kappaB to

297 eporter constructs, we show that cytoplasmic unspliced XBP1 mRNA is efficiently spliced by activated

298 ieve repression by XBP1u, the product of the unspliced Xbp1 mRNA.

299 XBP1 splicing, occurring after depletion of unspliced XBP1.

300 Using tiling arrays, we show that many unspliced yeast pre-mRNAs accumulate in strains mutated