ライフサイエンスコーパス: urease

1 protected the activity of the electrosprayed urease.

2 llow the rapid access of urea to cytoplasmic urease.

3 mouse models to examine the contribution of urease.

4 more susceptible to fungi in the absence of urease.

5 zymes, Salmonella hydrogenase and Klebsiella urease.

6 mbly of the nickel-containing active site of urease.

7 eakage allowing fluoride ions easy access to urease.

8 ature coupled with pH on the activity of the urease.

9 d in the maturation of the nickel-containing urease.

10 oorganisms: the hydrolysis of urea by enzyme urease.

11 orosarcina pasteurii and Helicobacter pylori ureases.

12 Important components include a cytoplasmic urease; a pH-gated urea channel, UreI; and periplasmic a

13 acterization of Glycine max UreG (GmUreG), a urease accessory protein.

14 In plants, its activation requires three urease accessory proteins (UAPs), UreD, UreF, and UreG.

15 Urease accounts for up to 10% of the total cellular H. p

16 structed for studying the in vivo effects on urease activation in recombinant Escherichia coli cells,

17 l for nickel delivery because UreG-dependent urease activation in vitro was observed only with UreG o

18 mes available in the leaves, indicating that urease activation is limited by nickel accessibility in

19 f UreG for nickel delivery during eukaryotic urease activation, inciting further investigations of th

20 Nickel is inserted into the urease active site in a GTP-dependent process with the a

21 e, closely resembling the traditional nickel-urease active site.

22 ter is found to be associated with different urease activities and, consequently, precipitation kinet

23 Average urease activity after manure was applied was shown to be

24 that promoted a long-term reduction in fecal urease activity and ammonia production.

25 nism, was sufficient to enhance P. mirabilis urease activity and increase disease severity, and enhan

26 t nickel import is critical for P. mirabilis urease activity and pathogenesis during infection.

27 environments, very little is known about the urease activity and regulation in specific bacteria othe

28 ere engineered to display either high or low urease activity and the native producer Sporosarcina pas

29 Using an in vitro urease activity assay, it is shown that the recombinant

30 i were also significantly higher for the low-urease activity E. coli compared with the high-urease ac

31 ease activity E. coli compared with the high-urease activity E. coli.

32 binant protein is sufficient for recovery of urease activity in cell lysate from a HypA deletion muta

33 ur expectations, ammonia availability due to urease activity in P. mirabilis did not drive this gene

34 mirabilis and P. stuartii increased overall urease activity in vitro and disease severity in a model

35 We demonstrate that the lack of urease activity in vitro is not solely due to the amber

36 ther uropathogens also enhanced P. mirabilis urease activity in vitro, including recent clinical isol

37 The total urease activity level was decreased in the DeltaarsS str

38 de that the underlying mechanism of enhanced urease activity may represent a widespread target for li

39 rotein were analyzed by Western blotting and urease activity measurements.

40 Urease activity potentially assists with survival fitnes

41 TI), delineated the contribution of enhanced urease activity to coinfection pathogenesis, and screene

42 three microorganisms produced calcite, lower urease activity was associated with both slower initial

43 Furthermore, total urease activity was increased during co-culture.

44 The urease activity was induced by urea, since complete and

45 and increase disease severity, and enhanced urease activity was the predominant factor driving tissu

46 tion pathogenesis, and screened for enhanced urease activity with other common CAUTI pathogens.

47 icroorganisms genetically engineered for low urease activity would achieve larger calcite crystals wi

48 y, (1) exhaled antigen 85, (2) mycobacterial urease activity, and (3) detection by trained rats of di

49 nd nikA to virulence is due to their role in urease activity, as neither mutant exhibited a fitness d

50 ia binding of Ag(I), allowing restoration of urease activity, hydrolysis of urea to produce ammonia,

51 In contrast to urease activity, IR treatment had a more pronounced effe

52 other uropathogens can enhance P. mirabilis urease activity, resulting in greater disease severity d

53 e engineered murine gut microbiota to reduce urease activity.

54 growth dynamics and impact of co-culture on urease activity.

55 russels sprouts) plants juices, on jack bean urease activity.

56 ast in part through synergistic induction of urease activity.

57 ontain a mutated ureD allele that eliminated urease activity.

58 creases the 2.7-kb transcript, and decreases urease activity.

59 ding motif result in a decrease in recovered urease activity.

60 in a contact-independent manner to decrease urease activity.

61 monia with ninhydrin, was developed to study urease activity.

62 lori requires two nickel-containing enzymes, urease and [NiFe]-hydrogenase, for efficient colonizatio

63 e extracytoplasmic function-sigma factors, a urease and a bile salt hydrolase.

64 in this study contribute to acid resistance (urease and amidase), acetone metabolism (acetone carboxy

65 In general, they also showed poor anti-urease and anti-tyrosinase activities.

66 N. moscoviensis possesses genes coding for a urease and cleaves urea to ammonia and CO2.

67 The EIS-urease and EIS-(PAMAM/CNT)-urease sensors showed similar

68 In bacteria, the UAPs interact with urease and facilitate activation, which involves the cha

69 creating the biosensor systems, the enzymes urease and glucose oxidase (GOD) were used as a bioselec

70 In the four cases studied (catalase, lipase, urease and glucose oxidase), the flow is driven by a gra

71 d in virulence phenotypes including laccase, urease and growth under oxidative/nitrosative stress.

72 of water-stable aggregate and activities of urease and invertase compared to conventional nitrogen r

73 require two virulence factors in particular: urease and MR/P fimbriae.

74 equires the nickel-containing metalloenzymes urease and NiFe-hydrogenase to survive this low pH envir

75 New urease and nitrification inhibitors and polymer coatings

76 nhibitors UI, and double inhibitors DI, i.e. urease and nitrification inhibitors combined) regarding

77 UAN treated with NBPT(3) or a combination of urease and nitrification inhibitors resulted in 16.5-16.

78 ally generated during H. pylori infection by urease and other enzymes, enhance VacA toxicity by inhib

79 Both the urease and the T3SS were previously shown to be essentia

80 treatment caused a substantial reduction in urease and trypsin inhibitor activities and considerable

81 is, the enzymatic activities of arginase and urease, and fungal gene expression in the extraradical a

82 acetylcholinesterase, butyrylcholinesterase, urease, and tyrosinase enzymes.

83 i2+ ions into the nascent active site of the urease apoprotein (UreABC).

84 The results obtained with jack bean urease as a model urease, may contribute to the understa

85 nization and denaturation of the immobilized urease, as such the model includes the effect of the fix

86 ing that Zn(2+) may play a role on the plant urease assembly process, as suggested for bacteria.

87 ly onto a bare EIS [EIS-urease] sensor; (ii) urease atop the LbL film over the EIS [EIS-(PAMAM/CNT)-u

88 pylori phosphoglucosamine mutase (glmM) and urease B (ureB) gene of H. heilmannii and H. felis.

89 is induced by LPS from H. pylori, while the urease B subunit (UreB) is required for NLRP3 inflammaso

90 this study are comparable to the commercial urease based detection methods.

91 tiotemporal variation in pumping behavior in urease-based pumps and uncover the mechanisms behind the

92 onstrated, surpassing the conventional NWFET urease-based readout.

93 erent molecular systems, we investigated the urease-bound aggregation kinetics of the Au and Ag NPs w

94 HP eradication was confirmed using urease breath test and stool antigen test.

95 Primers of urease C gene of H. pylori and Sau-3 and Hha I restricti

96 nd not absolutely required for colonization, urease can contribute to the pathogenicity of STEC.

97 iencies, such as chymotrypsin, fumarase, and urease, can be accurately and precisely estimated from a

98 The urease-catalyzed hydrolysis of hydroxyurea is known to e

99 The urease-catalyzed hydrolysis of urea displays feedback th

100 rease in pH after an induction period in the urease-catalyzed hydrolysis of urea was used to trigger

101 strain, a point mutation in ureD, encoding a urease chaperone protein, was identified, resulting in a

102 of a nonlinear pH ramp generated by the urea-urease clock reaction.

103 extracts and able to complement a DeltaureD-urease cluster in this host microorganism.

104 This analysis revealed that the urease cluster was the predominant factor in mediating r

105 On the other hand, the EIS-(PAMAM/CNT)-urease-CNT sensor exhibited a superior output signal per

106 film and another CNT layer [EIS-(PAMAM/CNT)-urease-CNT].

107 ROF electrodes were reversibly modified with urease-coated magnetic microparticles (MP) using a magne

108 complex was observed in cells producing all urease components.

109 me increase in the urea concentration in the urease-containing reaction medium.

110 In the intestine, bacterial urease converts host-derived urea to ammonia and carbon

111 central nervous system in a process that is urease dependent, requires viability, and involves cellu

112 These complications were urease-dependent as they were not observed during coinfe

113 on promotes urolithiasis and bacteremia in a urease-dependent manner, at least in part through synerg

114 experimental CAUTI, predominantly through a urease-dependent mechanism.

115 . pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher

116 gher in the protein extracts from transgenic urease-deprived plants than in extracts from non-transge

117 Urease diffusion measured using fluorescence correlation

118 The soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling m

119 d on Ure protein sequences from a variety of urease-encoding bacteria demonstrates that the proline a

120 Furthermore, M. morganii actively prevents urease enhancement by E. faecalis, P. stuartii, and E. c

121 However, urease enzymatic activity is not related to its toxicity

122 test has been developed using modulation of urease enzyme activity for detection of C-C mismatch sin

123 s reveal that the L38P substitution enhances urease enzyme activity; however, the L205P substitution

124 er anoxic conditions is a consequence of the urease enzyme already present in the cells of the aerobi

125 In this study, we identify the Yersinia urease enzyme as the responsible oral toxin.

126 ut repeated injection of S. pasteurii as the urease enzyme degrades and/or becomes inhibited.

127 The STEC urease enzyme exhibited maximum activity near neutral pH

128 We show that diffusion of single urease enzyme molecules increases in the presence of ure

129 ion gradient, we show that both catalase and urease enzyme molecules spread toward areas of higher su

130 mposition of urine and the prevalence of the urease enzyme that hydrolyzes urea, minerals readily pre

131 ed soil improvement technique that uses free urease enzyme to catalyze the hydrolysis of urea in an a

132 al. (2015) report that H. pylori employs its urease enzyme to destroy urea to bring the concentration

133 regulation of the T3SS and activation of the urease enzyme, either of which would prevent E. ictaluri

134 The urease enzyme, encoded in the STEC chromosome, has been

135 e particularly challenging due to its potent urease enzyme, which facilitates formation of struvite c

136 One important virulence factor is its urease enzyme, which requires nickel to be catalytically

137 importing nickel for incorporation into the urease enzyme.

138 me produces the urea used by the E. ictaluri urease enzyme.

139 had no effect on UreG's ability to activate urease, enzyme activity was essentially abolished in the

140 e possible by soil microorganisms containing urease enzymes, which hydrolyze urea and enable carbonat

141 l that, although the benefit STEC gains from urease expression is modest and not absolutely required

142 sRNA103, and sRNA350--were shown to regulate urease, fimbria, and the LEE, respectively.

143 nanoparticles (NPs) aggregates of commercial urease from jack beans (Canavalia ensiformis) were prepa

144 We hypothesize that the gain and loss of the urease function is related to transmission strategies an

145 For future work, overexpression of urease fungitoxic peptides could be attempted as an alte

146 ve manner, controls the transcription of the urease gene cluster and alpha-carbonic anhydrase.

147 separate operons (ureAB and ureIEFGH) of the urease gene cluster.

148 ates transcription of the seven genes of the urease gene cluster.

149 efined consortium of 8 bacteria with minimal urease gene content.

150 ArsS, in addition to its role in urease gene transcription, is also involved in the recru

151 The urease genes are also present in Brucella, which has a f

152 The presence of highly similar urease genes in Nitrospira lenta from activated sludge,

153 are based on distributions of thaumarchaeote urease genes rather than activity measurements.

154 Arctic deep waters had a higher abundance of urease genes than those near the surface suggesting gene

155 ze ammonia, and a large fraction of them had urease genes, enabling the use of urea to fuel nitrifica

156 with enzymes and/or other biomolecules, e.g. urease, glucose oxidase, hemoglobin, myoglobin (Mb), con

157 k, a combination of creatinine deaminase and urease has been chosen as a model system to demonstrate

158 Molecular dynamics simulations of urease have revealed a previously unobserved wide-open f

159 The NH(3) produced as urease hydrolyzes urea can aid in buffering bacteria in

160 ture, involving the LbL film with the enzyme urease: (i) urease immobilized directly onto a bare EIS

161 ing the LbL film with the enzyme urease: (i) urease immobilized directly onto a bare EIS [EIS-urease]

162 lass of bifunctional inhibitors of bacterial ureases, important molecular targets for antimicrobial t

163 reD and then into the nascent active site of urease in a GTP-dependent process.

164 rrets, synthesizes a distinct iron-dependent urease in addition to its archetypical nickel-containing

165 ar behavior was also observed for the enzyme urease in aqueous solution.

166 the activity of the Ni(II)-dependent enzyme urease in bacterial cultures.

167 The increased level of urease in gastric acidity is due, in part, to acid activ

168 The increased level of urease in gastric acidity is due, in part, to acid activ

169 Here, we explore the role of urease in STEC pathogenicity.

170 soils and freshwater habitats, and of other ureases in marine nitrite oxidizers, suggests a wide dis

171 Urease inactivation in unsoaked samples was achieved at

172 Healthy urine is around pH 6, bacterial urease increases urine pH leading to the precipitation o

173 Therefore, urease inhibition by manuka honey is mainly due to MGO a

174 Urease inhibition of manuka honey correlates with its MG

175 concentration, as well as the efficiency of urease inhibition.

176 lack MGO and DHA, showed significantly less urease inhibition.

177 on from manuka honey with glyoxalase reduced urease inhibition.

178 es on corn can be enhanced with an effective urease inhibitor in areas and years with noticeable urea

179 The use of the urease inhibitor N-(n-butyl) thiophosphoric triamide (NB

180 NCP to test the ability of a newly developed urease inhibitor product Limus((R)) to decrease NH3 vola

181 of the examined organoselenium compounds as urease inhibitors against pathogenic bacteria.

182 Urease inhibitors are considered promising compounds for

183 ertilizers PCF, nitrification inhibitors NI, urease inhibitors UI, and double inhibitors DI, i.e. ure

184 n manuka honey, were identified as jack bean urease inhibitors with IC50 values of 2.8 and 5.0mM, res

185 represent a class of competitive reversible urease inhibitors.

186 nose-resistant Proteus-like (MR/P) fimbriae, urease, iron uptake systems, amino acid and peptide tran

187 Urease is a ubiquitous nickel metalloenzyme.

188 Urease is an essential component of gastric acid acclima

189 ic yeast Hansenula polymorpha and commercial urease is described.

190 Expression of urease is essential for gastric colonization by Helicoba

191 Urease is first inactivated with silver ions and printed

192 Although the iron-urease is less active than the nickel-enzyme, its activi

193 The iron-urease is oxygen-labile, with the inactive protein exhib

194 s and product egress from the active site of urease is tightly controlled by an active-site flap.

195 reactive inhibitor of Sporosarcina pasteurii urease (k(inact)/K(I) = 10 420 s(-1) M(-1)).

196 Failure of an E. ictaluri urease knockout mutant to increase the ECV pH in the in

197 The urease levels and activity of the DeltaarsS strain after

198 anism and roles of each accessory protein in urease maturation are the subject of ongoing studies, wi

199 ry to [NiFe]-hydrogenase and participates in urease maturation.

200 ts obtained with jack bean urease as a model urease, may contribute to the understanding of bacterial

201 tions catalyzed by glucose oxidase (GOx) and urease, measurements of proteins by BCA assay, analysis

202 probe/sensor with the ability to detect the urease-mediated hydrolysis of urea in aqueous solution.

203 with the determination of urea in plasma by urease-mediated hydrolysis of urea.

204 fungal Mn biomineralization process based on urease-mediated Mn carbonate bioprecipitation [24].

205 This urease-mediated synthesis yields nearly monodisperse TiO

206 ated by FlgS, underscoring the importance of urease membrane recruitment and activation in periplasmi

207 Urease membrane recruitment following prolonged acid exp

208 Assembly of the Klebsiella aerogenes urease metallocenter requires four accessory proteins, U

209 In the presence of urea, the urease-modified rGO FETs showed a shift in the Dirac poi

210 Mice were infected with P. mirabilis or a urease mutant, P. stuartii, or a combination of these or

211 erved during coinfection with a P. mirabilis urease mutant.

212 ost and bacterial protein synthesis, but not urease, NapA, VacA, CagA, or CagT.

213 majority of EHEC strains are phenotypically urease negative under tested conditions.

214 hibited a fitness defect when disrupted in a urease-negative background.

215 During a UTI, urease-negative E. coli bacteria thrive, despite the com

216 A urease-negative, fusiform, novel bacterium named Helicob

217 gical roles have been demonstrated for plant ureases, notably in toxicity to other organisms.

218 The TEM images of urease NPs showed their size in the range, 18-100nm with

219 yer (LbL) assemblies of polyethylenimine and urease onto reduced-graphene-oxide based field-effect tr

220 Conversely, deletion of the urease operon in Y. pseudotuberculosis rendered it nonto

221 ble solute glycine betaine, ure genes of the urease operon, and mscL encoding a mechanosensitive chan

222 cell wall remodeling genes (lytN, ddh), the urease operon, genes involved in metal transport (feoA,

223 r cells, highly upregulated loci include the urease operon, the pyrimidine biosynthesis operon, the b

224 thaumarchaeal-related scaffold with the full urease operon.

225 7:H7 isolates and correlates with a negative urease phenotype in vitro.

226 tion, particularly by P. mirabilis and other urease-positive bacteria.

227 These findings suggest that urease-positive fungi could play an important role in th

228 lly induced mineralization, and suggest that urease-positive fungi may play a potential role in the s

229 The urease-positive fungi Pestalotiopsis sp. and Myrothecium

230 In this research, the urease-positive fungus Neurospora crassa was investigate

231 settings, and polymicrobial colonization by urease-positive organisms, such as Proteus mirabilis and

232 of the O157:H7 strains, neither the in vitro urease-positive phenotype nor the ureD sequence is phylo

233 The urease-positive species Proteus mirabilis and Providenci

234 rformed with three phylogenetically distinct urease-positive strains demonstrated that the STEC ure g

235 eitol, a total reactivation of the inhibited urease proceeded for the tested plants except for the on

236 The enzyme urease produces ammonia and enables bacteria to adapt to

237 Urease produces NH(3) and CO(2), neutralizing entering p

238 Proteus mirabilis is a model organism for urease-producing uropathogens.

239 The presence of P. stuartii, regardless of urease production by this organism, was sufficient to en

240 tored through omeprazole, demonstrating that urease production in L. reuteri is mainly devoted to ove

241 be identified, but genes for a multi-subunit urease protein complex are present in the genome.

242 exposure increased the level and activity of urease proteins at the membrane in the wild type but not

243 tion, is also involved in the recruitment of urease proteins to the inner membrane to augment acid ac

244 medically important dinuclear nickel enzyme urease provides an excellent model for studying metalloc

245 We also show that urease reaches its full activity several days after nick

246 assay suggests that ammonia produced by the urease reaction mediates the pH increase.

247 luding a Michaelis-Menten expression for the urease reaction with a bell-shaped rate-pH dependence.

248 The onion juice modified urease, regained only half of the initial activity.

249 Because urease-related mortality eliminates 30-40% of infective

250 low-molecular-weight inhibitors of bacterial ureases reported to date.

251 mM for creatinine deaminase and 0.139 mM for urease, respectively.

252 S [EIS-(PAMAM/CNT)-urease] sensor; and (iii) urease sandwiched between the LbL film and another CNT l

253 se immobilized directly onto a bare EIS [EIS-urease] sensor; (ii) urease atop the LbL film over the E

254 p the LbL film over the EIS [EIS-(PAMAM/CNT)-urease] sensor; and (iii) urease sandwiched between the

255 The EIS-urease and EIS-(PAMAM/CNT)-urease sensors showed similar sensitivity (~18 mV/decade

256 the nickel-based metallocenter of the enzyme urease; several of the processing, transport, and medica

257 Attraction to urea requires that H. pylori urease simultaneously destroys the signal.

258 mul saliva is needed to be mixed with 10 mul urease solution and 90 mul pH indicator solution in the

259 aqueous solution by taking advantage of the urease specific inhibition.

260 he ionization and denaturation states of the urease subject to the environmental conditions.

261 e the for metabolic processes important urea-urease system, with sensors based on spray-coated CNT-FE

262 121 consecutive patients had positive rapid urease test (RUT) and received STT.

263 ve endoscopy, histological assessment, rapid urease test and breath test were performed before and ei

264 ection and the HP virulence factor CagA by a urease test and PCR.

265 d biopsy histological examination plus rapid urease test indicating H. pylori infection; 2) gastric I

266 Unchanged substrate in a negative rapid urease test may be reused to detect Helicobacter pylori

267 The Rapid Urease Test was applied to fragments of the antral mucos

268 ., culture, histology Giemsa stain and rapid urease test) and non-invasive tests (anti-H. pylori IgG)

269 , 97%, 91%, and 66%, respectively, for rapid urease test).

270 nd NHPGH-infected groups, based on the rapid urease test, histological analysis of biopsies, and PCR

271 n esophagogastroduodenoscopy (EGD) for rapid urease test, histology and PCR examination for Helicobac

272 H. pylori status was determined by the urease test, histology and presence of H. pylori ureA.

273 followed by histology, culture, and a rapid urease test, in order to obtain a consensus patient infe

274 r pylori requires nickel for hydrogenase and urease that are essential for acid viability), the "traf

275 rdsiella ictaluri produces an acid-activated urease that can modulate environmental pH through the pr

276 These bacteria secrete the enzyme urease that converts urea into carbon dioxide (CO2) and

277 ed component of two enzymes, hydrogenase and urease, that have been shown to be important for in vivo

278 Urease, the enzyme responsible for urea hydrolysis, and

279 elicobacter pylori possesses a highly active urease to support acid tolerance.

280 Here we used a hydrolytic enzyme, urease, to modify the solution environment around a wate

281 lyaniline), or biocatalytic (glucose oxidase/urease) triggers.

282 Effects of IR treatment on urease, trypsin inhibitor, lipoxygenase-1 and lipoxygena

283 cussed, suggesting that de novo synthesis of urease under anoxic conditions is not possible and that

284 Ur/Nr-NiO/ITO/glass) after immobilization of urease (Ur) shows excellent biosensing response characte

285 ) indicates the high affinity of immobilized urease (Ur) towards the analyte (urea).

286 n efficient matrix for the immobilisation of urease (Ur), the specific enzyme for urea detection.

287 y expressed in planta, we demonstrate that a urease-UreD-UreF-UreG complex exists in plants and show

288 Urease (Urs) and glutamate dehydrogenase (GLDH) are cova

289 of DNA containing C-C mismatches reactivates urease via binding of Ag(I), allowing restoration of ure

290 ction is achieved by immobilizing the enzyme urease via certain surface functionalization techniques

291 s increase was significantly attenuated when urease was inhibited with pyrocatechol, demonstrating th

292 Despite the low yield of the ROS, jack bean urease was shown to be inactivated when the enzyme solut

293 uorescence lifetime of SNARF-1 conjugated to urease were not sufficient to explain the increase in di

294 n, columns with defined zones of immobilized urease were used to examine the distribution of calcium

295 also revealed an enrichment in orthologs of urease, which has been linked to dysbiosis and inflammat

296 ce factors required for cluster development: urease, which is required for urolithiasis, and mannose-

297 the urea sensors are biosensors and utilize urease, which limit their use in harsh environments.

298 c reactions catalyzed by glucose oxidase and urease, which results in a restoration of the photolumin

299 oviensis to supply ammonia oxidizers lacking urease with ammonia from urea, which is fully nitrified

300 ther demonstrated the coupling of the enzyme urease with the nano-PANI:PSS to create a urea biosensor