ライフサイエンスコーパス: variable domain

1 hain homodimers is mediated through a single variable domain.

2 somatic mutations distributed throughout the variable domain.

3 , important function for this evolutionarily variable domain.

4 able domain scaffold and a fixed light chain variable domain.

5 rotein, a dimeric immunoglobulin light chain variable domain.

6 b with the framework sequences of a human Ab variable domain.

7 l terminal ends, respectively, and a central variable domain.

8 ed by synthetic peptides derived from the LC variable domain.

9 iate via their amino-terminal immunoglobulin variable domain.

10 e amino and carboxyl termini and one central variable domain.

11 iable regions also may be present within the variable domain.

12 ffinity-labels a single site in the antibody variable domain.

13 ace receptor selects OME partners based on a variable domain.

14 each monomer comprising one constant and one variable domain.

15 r down into the combining site between the 2 variable domains.

16 h the interface of the heavy and light chain variable domains.

17 delta-H chains, which incorporated Cmu1 and variable domains.

18 sequences of antibody variable fragment (Fv) variable domains.

19 artilaginous fish and in camelid heavy-chain variable domains.

20 olves stabilization of heavy and light chain variable domains.

21 to the weak immunogenicity of immunoglobulin variable domains.

22 f the immunogenicity derived from the Fc and variable domains.

23 was found to be higher in CH1 and CL than in variable domains.

24 ficial mutations can be found throughout the variable domains.

25 h an antigen binding site made up of 2 kappa variable domains.

26 linkages at the base of the surface-exposed variable domains.

27 n might also depend on IgA carrying specific variable domains.

28 25% of serum IgG contains glycans within the variable domains.

29 induced change in the orientation of the two variable domains.

30 MAb MoPn-40 binds to a linear epitope in the variable domain 1 (VD1), and MAb MoPn-32 recognizes the

31 In many isolates variable domain 1 is flanked by direct repeat elements,

32 nce changes in gp160, principally within the variable domain 1/variable domain 2, variable domain 3,

33 und to be clustered in two distinct domains (variable domains 1 and 2).

34 60, principally within the variable domain 1/variable domain 2, variable domain 3, and variable domai

35 TCR ligand, human T-cell receptor beta chain variable domain 2.1.

36 though numerous changes were observed in the variable domain 3 loop and in other regions of gp160.

37 hin the variable domain 1/variable domain 2, variable domain 3, and variable domain 4 loops.

38 1/variable domain 2, variable domain 3, and variable domain 4 loops.

39 protein, TIGIT, containing an immunoglobulin variable domain, a transmembrane domain and an immunorec

40 One contains two variable domains: a somatically recombined V and a prejo

41 though the changes in the association of the variable domains affect the precise positioning of resid

42 SBT6.1-mediated cleavage within the variable domain allows for continued passage of the part

43 sseria gonorrhoeae include an immunodominant variable domain and one or more invariable domains that

44 d with random mutagenesis of its heavy-chain variable domain and panning against sGHeV.

45 Additional regions of the N-terminal variable domain and the constant domains, however, great

46 ion extended off the side of the light chain variable domain and was not required for neutralization.

47 ural and sequence similarities between mouse variable domains and a repertoire of human antibody germ

48 mational changes that expose epitopes in the variable domains and disrupt quaternary epitopes in the

49 the interface of the heavy- and light-chain variable domains and form the antigen-binding site, the

50 ntarity-determining region of immunoglobulin variable domains and has been shown to be conformational

51 e only gene with both central and C-terminal variable domains and has three to four times more intron

52 n the interfacial region of the constant and variable domains and highlight the general resistance of

53 ting Bence-Jones light-chain dimers in their variable domains and remain wild-type in their antibody

54 en to be near the linker regions between the variable domains and the constant domains of these antib

55 evel of sequence diversity observed in human variable domains and the requirement to maintain antigen

56 ubclass, containing the same antigen-binding variable domains and with equal binding to Abeta, MABT s

57 the development of human-based single chain variable domain antibody fragments (scFvs) directed agai

58 A model BsAb was constructed using a single variable domain antibody to mouse platelet-derived growt

59 get antigens, by genetically fusing a single variable domain antibody to the N terminus of the light

60 Here, we report a human heavy-chain variable domain antibody, HN3, with high affinity (Kd =

61 A bispecific, single variable-domain antibody (anti-TNFRI moiety plus an albu

62 ysis of these V-NARs has revealed an unusual variable domain-antigen interaction.

63 Somatic mutations within the antibody variable domains are critical to the immense capacity of

64 that have only heavy chains; unpaired heavy variable domains are responsible for antigen binding.

65 te the lack of a canonical hinge region, the variable domains are spaced appropriately wide for bindi

66 When expressed recombinantly these variable domains are termed single domain antibodies (sd

67 entarity determining region, suggesting that variable domains are the products of antigen-driven B ce

68 c form of the Mcg immunoglobulin light chain variable domain as the quaternary unit required for amyl

69 ons of homology in 3H1 heavy and light chain variable domains, as well as in the framework regions.

70 When using variable domain backbones from the crystal structures, t

71 We identify structurally variable domains between cell types and examine the unde

72 only in the diverse epitopes to which their variable domains bind but also in the various effector m

73 All heavy chain-only antibody variable domains bind HypE when expressed as GFP fusions

74 h by developing high-affinity Fv from single variable domains binding to RAS and LMO2 oncogenic prote

75 single-chain antibody component in which the variable domain binds to hFcgammaRI [anti-hFcgammaRI (H2

76 We demonstrate that each of the three variable domains binds to the same variable domain in an

77 The variable domain bound to virions and blocked HSV infecti

78 sociated heavy (V(H)) and light (V(L)) chain variable domains, but in camels and llamas, the binding

79 iversification of CDRs of the immunoglobulin variable domain by mutagenic PCR.

80 ve orientation of the heavy- and light-chain variable domains can create another source of structural

81 c LC showed high isoelectric point values of variable domain complementarity determining regions, pos

82 no acid residues in the framework of the TCR variable domains consistently increase the expression of

83 In contrast, large regions of the variable domain could be excised without reducing the in

84 CD) of 180-220 amino acids, and a C-terminus variable domain (CVD) of 30-60 amino acids.

85 soforms engage in homo-dimerization coupling variable domain D2 with D2, and D3 with D3.

86 volution of the previously defined EIAV gp90 variable domains demonstrated distinct differences in th

87 that Nanobodies, i.e. immunoglobulin single variable domains derived from naturally occurring heavy

88 tely 300 amino acids long) and an N-terminal variable domain differing in sequence and size (30-120 a

89 formation by stabilizing the Mcg light chain variable domain dimer and shifting the equilibrium away

90 Variable domain displayed no binding, and KH2 or KH3 dom

91 highly diversified library of human antibody variable domains (domain antibodies) and used it for sel

92 ve orientation of the light- and heavy-chain variable domains during evolution of the CH103 lineage.

93 his ARC platform is based on engineered dual variable domain (DVD) antibodies containing a natural un

94 ive natural buried lysine embedded in a dual variable domain (DVD) format.

95 ted for a dimeric immunoglobulin light chain variable domain, employing pressure, temperature, and so

96 Immunoglobulin gene variable domains encoding the antibodies that react with

97 nti-interleukin (IL)-6 antibody generated by variable domain engineering, to achieve subpicomolar aff

98 Positioning of incorrect Leu/Ile residues in variable domains, especially in CDRs (complementarity de

99 alculated selection conditions with antibody variable-domain exchange to direct individual antibody v

100 ly purified polypeptides, thereby permitting variable domain exon assembly by using this fully define

101 i-glycosyltransferases) and their N-terminal variable domain facing the cytosol.

102 33-CD8BBZ contains a fully human heavy-chain variable domain (FHVH) plus 4-1BB and CD3zeta domains.

103 rK ORFs shows the presence of seven discrete variable domains flanked by highly conserved regions.

104 of the Fab molecules and the sequence of the variable domain for each of the Fab molecules have been

105 aid in the selection of more suitable mouse variable domains for antibody engineering to render them

106 d limited cleavage sites are observed in the variable domains for each chain, where the S-S spans the

107 f K12v, suggesting synergism of KH2, KH3 and variable domains for the binding activity.

108 luenza-virus hemagglutinin (HA):single-chain variable-domain fragment complexes, by studying a comple

109 CR genes with the desired specificity, or Ab variable domain fragments fused with T cell-signaling mo

110 LEN is a kappaIV immunoglobulin light chain variable domain from a patient suffering from multiple m

111 GFR-iRGD consisting of an anti-EGFR VHH (the variable domain from the heavy chain of the antibody) fu

112 between the heavy (VH) and light (VL) chains variable domains from 15 to 5 amino acids.

113 stability of LC dimers and their associated variable domains from AL amyloidosis patients and non-pa

114 We cloned immunoglobulin G (IgG) variable domains from cryopreserved hybridoma cells and

115 ombinant immunotoxin containing an anti-CD22 variable domain (Fv) fused to truncated pseudomonas exot

116 encoded by several kappa and lambda germline variable domain gene segments.

117 onstructs are designed by combining antibody variable domains, generated by phage display or derived

118 t the organized assembly of antigen receptor variable domains, has been unclear.

119 We have used this intracellular single variable domain (IDab) format, based on a previously cha

120 Using dual variable domain Ig (DVD-Ig) technology, we constructed a

121 cells could not respond to the IgM/DNA dual variable domain Ig molecule, despite a normal response t

122 of bifunctional autoantibodies, IgM/DNA dual variable domain Ig molecules, to activate B cells throug

123 entical predominant V(H) gene with unmutated variable domain (IGHV1-3) for both IgG and IgM anti-HBc

124 in lacking two conserved cysteines in highly variable domain III of the extracellular region.

125 We developed a dual variable domain immunoglobulin of anti-CTLA4 antibody (a

126 oglobulin G (IgG)-like molecule--termed dual-variable-domain immunoglobulin (DVD-Ig)--that can be eng

127 anti-von Willebrand factor humanized single-variable-domain immunoglobulin (Nanobody), inhibits the

128 the three variable domains binds to the same variable domain in an opposing isoform and identify the

129 ective D gene incorporation into the TCRbeta variable domain in the absence of other nuclear factors

130 ne substrate specificity, whereas the larger variable domain in the N-terminus might play a role in f

131 l involvement of sites in both the V2 and V3 variable domains in antibody recognition.

132 ht into how "matching" at all three pairs of variable domains in Dscam mediates isoform-specific reco

133 ion of monoclonal immunoglobulin light chain variable domains in the form of insoluble amyloid fibril

134 Although much is known about the role of variable domains in the neutralization breadth and poten

135 s of extracellular deposition of light chain variable domains, including amyloid fibrils and amorphou

136 to facilitate incorporation of two different variable domains into a single molecule.

137 that affinity maturation of the light-chain variable domain is important for strong binding of the r

138 ion containing both NaCl and 6-AHA, only the variable domain is released from the particle surface.

139 myloid fibrils of immunoglobulin light-chain variable domains is proposed as a general model for the

140 mechanochemical core of Drp1, absent of the variable domain, is sufficient to mediate GTP hydrolysis

141 olypeptide sequences within their GTPase and variable domains, known as the A-insert and the B-insert

142 biophysical studies to probe immunoglobulin variable domain lambdaV6-57 V(L) aggregation, a process

143 The variable domain LEN was obtained from a patient who had

144 ly two of the 36 sequence differences in the variable domains, Leu(H47)Trp and Arg(H100)Trp, converts

145 rlap amino acids 64 to 104 of the N-terminal variable domain-like immunoglobulin domain.

146 We also show the variable domain limits premature Drp1 assembly in soluti

147 ween WbwK and WbsJ revealed that the smaller variable domains located in the C-terminus determine sub

148 Palpha1 directly binds to the immunoglobulin variable domain loop of purified human CD47 and that suc

149 cal studies demonstrate that, in addition to variable domain "matching," intramolecular interactions

150 Our data indicate that simple variable domain modifications at a distance from the ant

151 the expression of TCRs destabilized through variable domain mutation.

152 of active RalA is due in part to its unique variable domain near the C terminus.

153 A model of the variable domain of 16G3 was generated from the primary s

154 c model using site-directed insertion of the variable domain of a pathogenic human LC gene into the m

155 cooperativity in a complex formed between a variable domain of a T cell receptor and a bacterial sup

156 This variable domain of an H chain-only Ab (VHH or nanobody)

157 The variable domain of an immunoglobulin (IG) sequence is en

158 LEN is the variable domain of an immunoglobulin light chain origina

159 ide binding site (NBS), found within the Fab variable domain of antibodies, remains a not-so-widely k

160 Gene assembly of the variable domain of antigen receptors is initiated by DNA

161 l PKCs (C2-2), a peptide from the N-terminal variable domain of epsilonPKC (epsilonV1-2) and a peptid

162 amelids comprises a single domain, named the variable domain of heavy chain of HCAbs (VHH).

163 thout the intra-domain disulfide bond of the variable domain of heavy chain or the variable domain of

164 combining a chimpanzee IgG light chain and a variable domain of heavy chain with a human constant Fc

165 The resulting mRNA coding for the variable domain of heavy-chain antibodies (VHH) were iso

166 Amyloid fibrils from insulin and the variable domain of Ig light chain demonstrate induced CD

167 nts, VH, D, and JH, that together encode the variable domain of Ig.

168 of the variable domain of heavy chain or the variable domain of light chain.

169 n vitro reconstitution of BiP binding to the variable domain of light chains (VL).

170 synthetic phage display library based on the variable domain of new antigen receptor (VNAR) was used

171 In transfection studies, the isolated variable domain of PKC-epsilon selectively blocked exoge

172 he CDR3-like loop within the membrane-distal variable domain of Skint-1 (Skint-1 DV).

173 anscribed spacer (ITS) regions and the D1-D2 variable domain of the 28S ribosomal DNA gene (28S), the

174 her studied by computational modeling of the variable domain of the antibodies (variable fragment, Fv

175 We used high-throughput sequencing of the variable domain of the antibody heavy chain from 14 zebr

176 -domain TCR (svd TCR) that utilizes only the variable domain of the beta chain (Vbeta).

177 binding site is comprised exclusively of the variable domain of the heavy chain (VHH).

178 Nanobodies (Nbs), also known as the variable domain of the heavy-chain-only antibody (VHH),

179 The variable domain of the LC bears alone the structural pro

180 ementarity determining region (CDR) 3 of the variable domain of the light chain (VL) of this antibody

181 A recombinant variable domain of the light chain SMA was used to form

182 Antibodies directed to purified recombinant variable domain of Tpr K can opsonize T. pallidum, Nicho

183 ion of rabbits with the purified recombinant variable domain of Tpr K provides significant protection

184 ly showed that di-mannose recognition by the variable domains of 2G12 is independent of domain exchan

185 The variable domains of a high affinity anti-CEA antibody, T

186 issect how mutations at all positions of the variable domains of a high-affinity anti-VEGF antibody G

187 nd also by using intracellular expression of variable domains of a neutralizing antibody fused to gre

188 The variable domains of antibodies and T-Cell receptors (TCR

189 The cleavages are mainly localized in the variable domains of both heavy and light chains, the res

190 Unlike the monomeric variable domains of camelid heavy chain antibodies (V(H)

191 Variable domains of camelid heavy chain-only antibodies

192 nkage proximal glycosylation adjacent to the variable domains of gp120 and begin to explain how this

193 d cost-effective production, the recombinant variable domains of heavy-chain-only antibodies (VHHs) a

194 -bounded region spanning the V1 and V2 hyper-variable domains of HIV-1 gp120.

195 The variable domains of Ig and T-cell receptor genes in vert

196 High-throughput sequencing of the variable domains of immune receptors (antibodies and T c

197 hologic deposition of monoclonal light chain variable domains of immunoglobulins as insoluble fibrils

198 4G substitution, a mutation that is found in variable domains of lambda light-chain deposits in 25% o

199 , we designed anti-ETEC antibodies by fusing variable domains of llama heavy chain-only antibodies (V

200 lecules on biosensors was investigated using variable domains of llama heavy-chain antibodies (VHHs)

201 m2G7) was partly humanized (h2G7) by merging variable domains of m2G7 with human antibody-Fc backbone

202 Toxin binding to variable domains of T cell receptor beta chains (Vbeta)

203 rom a noncanonical interface between the two variable domains of the antibody.

204 "elbow" regions, which link the constant and variable domains of the Fab, can introduce disorder and

205 ith a high level of sequence homology in the variable domains of the heavy and light chains.

206 ormally single chain Fv fragments comprising variable domains of the immunoglobulin heavy (VH) and li

207 Nucleophilic sites in the paired variable domains of the light and heavy chains (VL and V

208 imers and probes were designed to target two variable domains of the ompA gene, VD2 and VD4.

209 from a hierarchical interaction between the variable domains of the OpaA protein of MS11mk.

210 mmunodominant epitopes of OspC reside in the variable domains of the protein.

211 First, in codons encoding highly variable domains of the proteins, there was a greater ac

212 ng site frequently comprises the heavy chain variable domain only (referred to as V(HH)).

213 ti-von Willebrand factor humanized, bivalent variable-domain-only immunoglobulin fragment, inhibits i

214 ity determining region loop conformation and variable domain orientation.

215 LT-1 is similar to other immunoglobulin-like variable domains, particularly those of triggering recep

216 es demonstrated that although its N-terminal variable domain plays an essential role in optimizing Ca

217 demonstrates that large portions of antibody variable domain positions are open to mutation, and that

218 known regarding how antibodies with a single variable domain recognize small ligands.

219 Dimers of Mcg variable domains remain stable and soluble, yet become p

220 The nanobodies were obtained from a variable-domain repertoire library isolated from llamas

221 interface within full-length LCs, utilizing variable-domain residues that are highly conserved in mo

222 matic mutations in the heavy and light chain variable domains, respectively, a long HCDR3, and a dele

223 l structure of the human CD84 immunoglobulin variable domain revealed an orthogonal homophilic dimer

224 fragments (scFvs) with a single heavy chain variable domain scaffold and a fixed light chain variabl

225 (anti-IC) single-chain fragment of antibody variable domain (scFv) and a monoclonal antibody capable

226 ation and characterization of a single-chain variable domain (scFv) antibody isolated against oligome

227 The IgG, diabody, single-chain variable domain (scFv), and novel miniantibody formats,

228 ic name, by a combination of metadata, or by variable domain sequence - returning all therapeutics th

229 lasts and amplified expressed immunoglobulin variable domain sequences by single-cell PCR.

230 C-terminal constructs, which lack central variable domain sequences, can oligomerize and localize

231 that builds downloadable homology models of variable domain sequences, tests them against our five d

232 , albeit with few somatic mutations in their variable domain sequences.

233 bility is the result of alternative usage of variable domain sequences.

234 mber antibody and T-cell receptor amino-acid variable domain sequences.

235 mbining site involving heavy and light chain variable domains shaped by somatic hypermutation and aff

236 ally folded intermediates of the light chain variable domain SMA in the presence of guanidine hydroch

237 enic kappa4 human immunoglobulin light-chain variable domain, SMA, associated with AL amyloidosis, we

238 y of a recombinant amyloidogenic light chain variable domain, SMA, on various surfaces was monitored

239 s of a recombinant amyloidogenic light chain variable domain, SMA, to determine whether partially fol

240 n of a recombinant amyloidogenic light-chain variable domain, SMA, with lipid vesicles.

241 integral to motor regulation is attached to variable domains so that the cell can target regulators

242 he functional paratopes with the 3D antibody variable domain structure as input.

243 Here, we model the variable domain structures of a large set of post-phase-

244 All the variable domains studied readily form amyloid fibrils, w

245 We describe a single-variable-domain TCR (svd TCR) that utilizes only the var

246 domain and a membrane-distal immunoglobulin variable domain that is responsible for ligand recogniti

247 Antigen-specific single immunoglobulin variable domains that bind to native antigens can be iso

248 ptors were prepared which contained antibody-variable domains that bound the capsid in place of the T

249 response by selecting mutations in antibody variable domains that enhance antigen binding.

250 ynamic coupling between the TCR constant and variable domains that is dampened upon ligation.

251 beta subunits displaying immunoglobulin-like variable domains that recognize peptide antigens associa

252 selection strategy to create human antibody variable domains that resisted heat aggregation.

253 zing protection from HIV infection via their variable domains, the antibody constant domain provides

254 This synaptogenesis depended on TCR variable domains, the kinase Lck and the integrin alpha(

255 Central variable domains themselves are monomeric and have no ta

256 In addition, interspersed within the VlsE variable domain there are six invariable regions (IR1-6)

257 mab, which are fused through the heavy chain variable domain to either cutinase or SnapTag, with a li

258 reverse turns, allows each pair of the three variable domains to "match" in an antiparallel fashion.

259 omain exchange to direct individual antibody variable domains to desired epitopes.

260 used with immunoblotting and sequencing IgG variable domains to screen, select, and characterize ant

261 e orientations of the heavy- and light-chain variable domains using side-chain rotamer sampling in th

262 emonstrated the ability to sequence antibody variable domains using the Ion Torrent PGM platform.

263 iduals, including one encoded by heavy-chain variable domain V(H)6-1.

264 gainst an Env and found that the heavy chain variable domain (V(H)) of this antibody, designated as m

265 y germline mutations only in the heavy chain variable domain (V(H)) that ultimately led to an increas

266 g an in vitro-evolved autonomous heavy chain variable domain (V(H)H-RIG), we have investigated the li

267 The antibody light chain variable domain (V(L))(1) and myelin protein zero (MPZ)

268 utations of human immunoglobulin light chain variable domain (V(L)).

269 third hypervariable loop of the light chain variable domain (V(L))] adopts the type 1 canonical stru

270 quencing of individual B cell immunoglobulin variable domains (V genes).

271 bodies consists of the heavy and light chain variable domains (V(L) and V(H) domains).

272 c assays demonstrated a distinct role of the variable domain V1/V2 in competitive resistance to CCR5

273 diversity among them was observed in the two variable domains (V1 and V2), semivariable domain (SV),

274 es targeting specific epitopes in the second variable domain (V2) of the HIV gp120 envelope (Env) pro

275 ty to antibodies directed to epitopes in the variable domains (V2 and V3) that are buried in the pare

276 s included those at the interface of the TCR variable domains (Valpha and Vbeta) and surface-exposed

277 ryl due to unpaired cysteine residues in the variable domains varied for different antibodies.

278 the concave beta-sheet surface of its single variable domain (Vbeta) to "horizontally" grab the protr

279 lecules and T-cell receptor (TCR) beta-chain variable domains (Vbetas).

280 says, we show that removal of the regulatory variable domain (VD) in Drp1 enhances formation of a fun

281 bules, but the function of the corresponding variable domain (VD, or insert B) of Drp1 is unknown.

282 method to site-specifically label VHHs [the variable domain (VH) of a camelid heavy-chain only antib

283 ith the constant region (Fc) and heavy chain variable domain (VH) of IgG, respectively.

284 alysed the pairings of heavy and light chain variable domains (VH and VL) in 365 human IgG+ B cells f

285 oclonal antibody requires that complementary variable domains (VH or VL) bind to the same antigenic s

286 affected by the relative orientation of the variable domains, VH and VL.

287 y immunizing mice with a thermally denatured variable domain (VL) fragment of the human kappa4 Bence

288 d components consisting predominately of the variable domain (VL) or the VL plus up to approximately

289 bodies typically recognize haptens using two variable domains (VL and VH), much less is known regardi

290 ediates the assembly of antibody light chain variable domains (VLs) into a correspondent symmetric te

291 Uniquely, the TIM-1 immunoglobulin variable domain was also required for P-selectin binding

292 Peptides from both conserved and variable domains were capable of inducing MIP-1alpha, MI

293 ibril formation of LEN, a benign light chain variable domain, were investigated at physiological pH i

294 o predicting the relative orientation of the variable domains when building homology models of antibo

295 oca: EpsH contains a large beta-sheet in the variable domain, where GspG contains an alpha-helix.

296 chains and light chain fragments composed of variable domains, which aggregate into amyloid fibers.

297 We show that the X-region represents a variable domain whose size changes with telomere length,

298 amin, however, Drp1 contains an unstructured variable domain, whose function is not yet fully resolve

299 trategy for the generation of human antibody variable domains with increased aggregation resistance.

300 consisting of only a fully human heavy-chain variable domain without a light-chain domain.