ライフサイエンスコーパス: vestibule

1 the electrical field of the channel (in the vestibule).

2 egion of the Kv voltage sensor, forms in the vestibule.

3 sence of calcium, the mobile unit closes the vestibule.

4 in binds to the N-terminal domain, opening a vestibule.

5 ubstrate or product dsDNA in the ion channel vestibule.

6 TRPA1 via a binding site not situated in the vestibule.

7 their Bodipy moiety within the M1 allosteric vestibule.

8 le for interaction with ligands entering the vestibule.

9 and in the flexibility of the extracellular vestibule.

10 and a radial expansion of the extracellular vestibule.

11 nd binding to a narrowing of the cytoplasmic vestibule.

12 o a cysteine introduced in the extracellular vestibule.

13 hair cells were in keeping with cells in the vestibule.

14 GABA-recognition site but faces the channel vestibule.

15 e (S2) binding site within the extracellular vestibule.

16 ruitments and selectivity by the periplasmic vestibule.

17 takes place as the drug associates with the vestibule.

18 res at a position deep within the inner pore vestibule.

19 rved residues located at the entrance of the vestibule.

20 in the nonsensory regions of the cochlea and vestibule.

21 he S2 site, located within the extracellular vestibule.

22 tive linker sequences similarly fold in this vestibule.

23 tor muscles and reestablish the depth of the vestibule.

24 6 helix packing at the narrowest part of the vestibule.

25 n of caesium ions bound in the extracellular vestibule.

26 overall 2-fold symmetry and a large central vestibule.

27 ochlea but not in the one that surrounds the vestibule.

28 meter of the protein, rather than inside the vestibule.

29 ntration of Ca(2+) ions in the extracellular vestibule.

30 t hairpins with 10-12 bp stems span the pore vestibule.

31 g a narrow constriction at the outer channel vestibule.

32 here one NH(4)(+) is captured in the binding vestibule.

33 above the macular sensory epithelium of the vestibule.

34 ansporter with a large (>45 A) extracellular vestibule.

35 A-wide channel, and a horn-shaped endofacial vestibule.

36 Cs, but significantly farther from the inner vestibule.

37 t maximum in the middle of the extracellular vestibule.

38 h the respective surface of the channel pore vestibule.

39 trin 1 is involved in canal formation of the vestibule.

40 ccompanying changes of polarity of the sugar vestibule.

41 50 muM to 100 nM when the toxin occupied the vestibule.

42 lvent-accessible at the extracellular domain vestibule.

43 nner ear and is connected to the cochlea and vestibule.

44 and TM6a toward TM3 and TM8 to close the EC vestibule.

45 above the entrance to the selectivity-filter vestibule.

46 ional modulation by membrane lipid and water vestibules.

47 ween hydrophilic periplasmic and cytoplasmic vestibules.

48 9) lie respectively in external and internal vestibules.

49 nnel pore and adjacent internal and external vestibules.

50 central histidine region, most likely in the vestibules.

51 iction between internal and external aqueous vestibules.

52 rrow tunnel flanked by wider inner and outer vestibules.

53 xternal Na(+) binding opened a large aqueous vestibule (600 A(3)) leading to the sugar-binding site.

54 ee orthogonal semicircular canals, a central vestibule, a coiled cochlea, and an endolymphatic duct a

55 other TCAs, binds in an extracellular-facing vestibule about 11 A above the substrate and two sodium

56 dicates that AK-42 binds to an extracellular vestibule above the channel pore.

57 allocation of the binding site to a luminal vestibule adjacent to Cys813 enclosed by part of TM4 and

58 a polymer is more likely to escape from the vestibule against the applied voltage gradient, while at

59 ted the duplex capture inside the nanopore's vestibule against the constriction region, subsequent cD

60 ibule but probably merging into the internal vestibule, allowing for control by the activation gate.

61 x region when it is captured in the alpha-HL vestibule, allowing the longer strand to translocate thr

62 esult from agonist binding to the allosteric vestibule alone, whereas the dualsteric binding mode pro

63 ptor by substituting several residues in the vestibule and adjacent regions from the rat receptor to

64 ting cells and hair cells of the cochlea and vestibule and also to cochlear hair cell nuclei and ster

65 rences in the conformation of the outer pore vestibule and associated glutamate gate may account for

66 ional fluctuations of the polymer inside the vestibule and beta-barrel compartments of the protein po

67 Translocation through the extracellular vestibule and binding of leucine in the leucine transpor

68 ating proteins specifically expressed in the vestibule and cochlea, respectively.

69 cked into a domain that connects the central vestibule and corner clamp region of RyR, resulting in a

70 revealing strong phylogenetic signal in the vestibule and enabling the proposal of potential synapom

71 murine ortholog, early in development in the vestibule and in hair cells and supporting cells of the

72 Lmo4 resulted in the dysmorphogenesis of the vestibule and in the absence of three semicircular canal

73 Two of these, the vestibule and intermediate sites, block (antagonize) the

74 A gap between the cytosolic vestibule and intramembrane groove provides a potential

75 rane, the T2SS has a much longer periplasmic vestibule and it has a short-lived flexible pseudopilus.

76 electrophoretically captured in the alpha-HL vestibule and positioned at the latch region, can be det

77 g triggers an expansion of the extracellular vestibule and stabilization of the open channel pore.

78 ained by contact points between hERG's outer vestibule and the bound BeKm-1 toxin molecule deduced fr

79 The large extracellular vestibule and the small-scale conformational changes cou

80 Ion permeation through the extracellular vestibule and the transmembrane channel is well understo

81 our external fenestrae to enter the rotundal vestibule and then cross one of four internal windows in

82 cidity of substrates entering the lipophilic vestibules and protonation via the bulk water.

83 ly, (b) receptor-bound VTD lies in the inner vestibule, and (c) VTD blocks this mutant channel as a b

84 zed in a more external position in the outer vestibule, and does not bind via direct coordination wit

85 he extracellular gate, open an intracellular vestibule, and largely disrupt the two sodium sites, thu

86 crease in hydrophobic side chains lining the vestibule, and this was reflected in solvation of the ch

87 tances to current flow in the baths, channel vestibules, and selectivity filter to change differently

88 eraction, and allowed ssDNA to reside in the vestibule approximately 100 times longer than the first

89 c antidepressants (TCAs) in an extracellular vestibule approximately 11 A above the bound leucine and

90 matic residues in the receptor extracellular vestibule, approximately 15 A from the classical, 'ortho

91 ing-related structural dynamics at the outer vestibule are not well understood.

92 lthough the structural dynamics of the outer vestibule are significantly altered during activation an

93 ents associated with establishing PCP in the vestibule are unclear, hindering data interpretation and

94 253), and a salt bridge in the extracellular vestibule (Arg-30 and Asp-404).

95 mutations in the dimer interface and channel vestibule as well as receptor composition.

96 stimulated by intense noise (middle ear and vestibule) as it was absent in CD1 mice with selective c

97 ubstitution of residues in the intracellular vestibule at positions 437, 438, 443, or 446 significant

98 Surprisingly, association with this vestibule, at a distance of 15 A from the binding pocket

99 ng liquid lidocaine compresses to the vulvar vestibule before penetration.

100 The cytoplasmic densities form a vestibule below the transmembrane domain with the C term

101 se of small molecule modulators, including a vestibule binding site that is not accessible in some pL

102 extrapolate the functional importance of the vestibule binding site to the human 5-HT(3) receptor, su

103 Four (out of eight) residues in the vestibule bound the dye, suggesting its role in substrat

104 only an inhibitor entry path to the luminal vestibule but also a channel leading to the ion binding

105 ive ion-conducting pore, bypassing the outer vestibule but probably merging into the internal vestibu

106 cupying the selectivity filter and cytosolic vestibule, but not the inner chamber.

107 domain 1a and occlusion of the extracellular vestibule by extracellular loop 4.

108 he selectivity filter and are trapped in the vestibule by the X-gate, which explains their exceptiona

109 gative electrostatic field shifted the outer vestibule carboxylate pK(a) into the physiological range

110 ward-occluded states, with the extracellular vestibule closed and the intracellular portion of transm

111 laryngeal movements contribute to laryngeal vestibule closure and upper oesophageal sphincter openin

112 te binding site located in the extracellular vestibule comprised of residues shown recently to partic

113 The duration of the vestibule configuration depends on polymer composition a

114 d voltage also increases the duration of the vestibule configuration.

115 base-specific interactions of dsDNA with the vestibule constriction "latch", a previously unrecognize

116 We propose a model in which this vestibule controls the entry and efflux of agonists from

117 s formed by D4938 and D4945 in the cytosolic vestibule determine RyR ion fluxes.

118 d to the conduction pathway within the outer vestibule did not directly contribute to the relevant lo

119 backbone carbonyl groups at the periplasmic vestibule direct NH4(+) to the conserved aromatic cage a

120 rroborating the conclusion that the internal vestibule does not harbor a gate.

121 Moreover, the outer vestibule does not significantly contribute to ion selec

122 pore and being preceded by entrapment in the vestibule domain of the alpha-HL.

123 otional restriction experienced by the outer vestibule during inactivation gating.

124 d C-terminal M4 transmembrane helices at the vestibule entrance.

125 TRPV1 reports distances in the extracellular vestibule, equivalent to those observed in the apo TRPV1

126 de of Tk-hefu-2 binding to the channel outer vestibule experimentally by site-directed mutagenesis.

127 Perturbing the conformation of outer vestibule/external pore entrance (by cysteine substituti

128 DraNramp, one lining the wide intracellular vestibule for metal release and the other forming a narr

129 ng data interpretation and employment of the vestibule for PCP studies.

130 nosine binds transiently to an extracellular vestibule formed by ECL2 and the top of TM5 and TM7, pri

131 pathway is characterized by a flexible outer vestibule formed by the TM1-TM2 loop, which leads to a n

132 phenylethylamino group, binds in an extended vestibule formed from transmembrane regions 2 and 7 (TM2

133 molecular dynamics simulations, hydrophilic vestibules formed by the N and C domains and in the intr

134 of F107 and F215, separating the periplasmic vestibule from the hydrophobic lumen, flip open and clos

135 that active glucose accumulation within the vestibule generates water flows simultaneously with the

136 l negatively charged residues from the upper vestibule had no effect on zinc inhibition.

137 Moreover, this vestibule hairpin is consistent with a closed-state conf

138 e direction from which the duplex enters the vestibule if the stabilities of leading base pairs at th

139 bottle-like central channel with the narrow vestibule in the cytoplasmic part covered by a ring of 5

140 Cd(2+) binding deep within the intracellular vestibule in the open state.

141 the bilayer and is connected to a cage-like vestibule in the periplasm.

142 Mutations in the extracellular vestibule in the SYTANLAAF motif disrupt the inhibitory

143 g to sites 1 and 2 of a K+ channel becomes a vestibule in which ions can diffuse but not bind specifi

144 llular domains, with a funnel-like exofacial vestibule (infundibulum), followed by a 15 A-long x 8 A-

145 tion is to keep the structure of periplasmic vestibule intact.

146 phobicity and wettability of their pores and vestibule interiors.

147 siding at the periphery of the extracellular vestibule, interposed between extracellular loops 4 and

148 ults suggest that the AMPA receptor external vestibule is a viable target for new positive allosteric

149 The outer vestibule is an important structurally extended region o

150 ether a-go-go related gene's (hERG's) outer vestibule is critical for its channel function: point mu

151 The structural widening of the extracellular vestibule is directly coupled to the opening of the ion

152 nts at SERT and suggest that the role of the vestibule is evolutionarily conserved among neurotransmi

153 omain (TM2), although a segment of the outer vestibule is formed by residues of TM1.

154 e inner ear, composed of the cochlea and the vestibule, is a specialized sensory organ for hearing an

155 conserved aromatic cage at the bottom of the vestibule (known as the Am1 site).

156 The cytosolic vestibule leads into a lumenally-sealed, lipid-exposed i

157 ier to ion flow and render the intracellular vestibule less splayed during channel opening in the pre

158 sed-blocked ion channel, a pyramidal central vestibule lined by residues implicated in binding ion ch

159 hydrophobic residues near the extracellular vestibule (local).

160 the allosteric binding in the extracellular vestibule located above the central substrate binding (S

161 ally (N- to C-terminus) only in a permissive vestibule located in the last 20 A of the tunnel.

162 seven additional humanizing mutations in the vestibule-located binding site of AChBP to improve its s

163 s novel binding site location in the central vestibule may also be relevant for structurally similar

164 This modified channel vestibule may also explain the dominant-negative effect

165 en-bonding networks within the extracellular vestibule may facilitate the transmission of cooperativi

166 Opening and closing of the vestibule might regulate access of substrates to the car

167 -resolution crystal-structure analysis, pore vestibule modeling, and structure-guided protein enginee

168 ts leading to restricted access to the upper vestibule, movement in the ion conducting lateral portal

169 ntitative polymerase chain reaction in nasal vestibule, nasal turbinate mucosa, and peripheral blood

170 demonstrate that both residues lie in a wide vestibule near the mouth of the pump's ion pathway.

171 y attract cations, through fenestrations, to vestibules near the ion channel.

172 In this vestibule, NH(4)(+) loses one water of hydration, but th

173 ns between short single-stranded DNA and the vestibule of a biological pore.

174 aller B-form duplexes (d = 2.0 nm) enter the vestibule of alphaHL, resulting in decreased current blo

175 on permeation possibly by clogging the inner vestibule of both PIEZO1 and PIEZO2.

176 nitially stabilized within the extracellular vestibule of Cys-loop receptors, and this stabilization

177 ucose exit from preloaded cells depletes the vestibule of glucose, making it hypotonic and thereby in

178 dentified two specific residues in the inner vestibule of K(Ca)2.3 (Ser507 and Ala532) that determine

179 thylammonium (TEA) binding site in the outer vestibule of K+ channels, and the mechanism by which ext

180 ed, SSRIs and TCAs bind in the extracellular vestibule of LeuT and act as non-competitive inhibitors

181 uctural reconfiguration of the extracellular vestibule of LeuT in which a "water gate" opens through

182 spontaneous transition of the extracellular vestibule of LeuT into an outward-open conformation.

183 hly conserved ring of charge in the external vestibule of mammalian voltage-gated sodium channels, un

184 nsight into the 3D architecture of the outer vestibule of NaV through a systematic structure-activity

185 uter vestibule residues shows that the outer vestibule of open/conductive conformation is highly dyna

186 computational docking located in the central vestibule of P2X7 involving S60, D318, and L320 in the l

187 at or near the limiting aperture within the vestibule of the alpha-hemolysin pore.

188 harged amino acids facing the ion permeation vestibule of the channel in question.

189 ts it from reaching its receptor site at the vestibule of the channel pore.

190 ly charged lysine residue (K95) in the inner vestibule of the channel pore.

191 )1.7 inhibitor that blocks the extracellular vestibule of the channel with an IC(50) of 72 nM and gre

192 ighly conserved residue in the extracellular vestibule of the channel, as the major element responsib

193 t the mutation, occurring near the cytosolic vestibule of the channel, reduces CDI as one of its prim

194 NR3A form a narrow constriction in the outer vestibule of the channel, which prevents passage of exte

195 de of the "propeller" blade toward the inner vestibule of the channel-and the C-terminal domain (CTD)

196 e four superficial carboxylates in the outer vestibule of the channel.

197 tion in negative charge at the extracellular vestibule of the channel.

198 lpha-helix organization near the cytoplasmic vestibule of the channel.

199 n of eight positive charges within the outer vestibule of the conduction pathway had no effect on the

200 Finally, the expanded luminal vestibule of the E2P model explains high-affinity ouabai

201 model for the open conformation of the outer vestibule of the hERG channel, in which the S5-P linkers

202 oth sensory and non-sensory formation of the vestibule of the inner ear.

203 tached by the tetrameric NT to the cytosolic vestibule of the InsP3R pore.

204 the transmitter binding site and (2) in the vestibule of the ion channel near the level of the trans

205 ta subunit interface and gammaTyr-105 in the vestibule of the ion channel, with photolabeling of both

206 esent at a specific location in the external vestibule of the ion-conducting pore.

207 pre-formed binding site in the extracellular vestibule of the iperoxo-bound receptor, inducing a slig

208 common" allosteric site in the extracellular vestibule of the M1 mAChR, suggesting that its high subt

209 Here, the extracellular vestibule of the M2 muscarinic acetylcholine receptor (m

210 esthetic derivative QX-222 into the internal vestibule of the mammalian rNaV1.4 channel.

211 recisely localized within the biotin-binding vestibule of the monovalent scdSav.

212 Electrostatic surface potentials in the vestibule of the nicotinic acetylcholine receptor (nAChR

213 d oral cavity (n = 46 [93%]) and pharynx and vestibule of the nose (n = 26 [53%]).

214 of the green mamba snake, binds in the outer vestibule of the pore and, like Ca2+, is a positive modu

215 h cysteine-substituted residues in the outer vestibule of the pore of ASIC1a.

216 se intersubunit disulfide bonds in the outer vestibule of the pore, Y424C-G428C transitions between t

217 ular "gating element" with structures at the vestibule of the pore.

218 the channel that contribute to the internal vestibule of the pore.

219 nct binding mode involving the central upper vestibule of the receptor in addition to the intersubuni

220 tions that an allosteric site located in the vestibule of the receptor offers an attractive target fo

221 transmitter binding site and the allosteric vestibule of the receptor protein.

222 te of action is located at the extracellular vestibule of the receptor's ion channel pore and is acce

223 A second site is located in the vestibule of the receptor, in a preexisting intrasubunit

224 y in an extended conformation in the folding vestibule of the ribosome yet ultimately emerges at the

225 s located within a Debye length of the outer vestibule of the SACs, but significantly farther from th

226 ansmembrane domains (TMD) from the cytosolic vestibule of the Sec61 channel into the lipid bilayer.

227 glutamine hypothesized to line the cytosolic vestibule of the skeletal muscle RyR (RyR1).

228 These findings establish the extracellular vestibule of the sodium channel as a viable receptor sit

229 gating domains surrounding the intracellular vestibule of the tetrameric central pore.

230 we propose an antagonist-binding site in the vestibule of the TRPA1 ion channel.

231 ligand (MRS2500) binds to the extracellular vestibule of this GPCR, whereas another (BPTU) occupies

232 tion that the TM5-TM6 linker forms the outer vestibule of TRPV1 channels and that AG489 is a pore blo

233 near-equal expression levels in cochlea and vestibule of wild-type and Va(J) mutants.

234 Both vestibules of the channel are strongly electronegative,

235 c receptors, initially making contact with a vestibule on each receptor's extracellular surface.

236 Access to the pore is through a vestibule on the cytosolic side that is fenestrated by s

237 m duplex (d = 2.4 nm) is unable to enter the vestibule opening of alphaHL on the cis side, leading to

238 oup of Arg-189 points toward the periplasmic vestibule, opening up the constriction to accommodate th

239 involving residues from the SF, outer-mouth vestibule, P-helices, and S5-P segments.

240 charged reagents indicate that a wide outer vestibule penetrates deep into the Na+,K+-ATPase, where

241 formation with water-accessible intrasubunit vestibules penetrating from the extracellular end all th

242 hibitor, traps nascent TMDs in the cytosolic vestibule, permitting detailed interrogation of an early

243 he selectivity filter, pore helix, and outer vestibule play a crucial role in gating mechanisms.

244 The results show that the periplasmic vestibule plays a crucial role in solute selectivity, an

245 a ring of negative charge within the central vestibule, poised to contribute to cation selectivity.

246 s a "gating-modifier": it binds to the outer vestibule/pore entrance of hERG and increases current am

247 bunit interfaces that create the cytoplasmic vestibule portals.

248 the R domain docked inside the intracellular vestibule, precluding channel opening.

249 eed, mutagenesis of selected residues in the vestibule reduces the allosteric potency of (S)-citalopr

250 idues for positively-charged residues in the vestibule region exhibited a factor of approximately 20

251 he formate substrate was unable to enter the vestibule region of EcYfdC.

252 senoside binding site in P2X4 in the central vestibule region of the large ectodomain.

253 figuration where a polymer occupies only the vestibule region of the pore, though a few appear relate

254 sidue and presence of acidic residues in the vestibule regions, conserved only in YfdC-alpha, were fo

255 nature of their interactions with the outer vestibule remains debatable, however.

256 of NH(4)(+) into the periplasmic recruitment vestibule requires only 3.1 kcal/mol of energy.

257 model of TRPA1 based on Kv1.2 to select pore vestibule residues available for interaction with ligand

258 nz-2-oxa-1,3-diazol-4-yl (NBD)-labeled outer vestibule residues shows that the outer vestibule of ope

259 direct coordination with any specific outer-vestibule residues.

260 tion of several residues in the hP2X2 middle vestibule resulted in dramatic changes in the potency of

261 hether the zinc-binding site lies within the vestibules running down the central axis of the receptor

262 tral binding (S1) site and the extracellular vestibule (S2 site).

263 ted to be located in the large extracellular vestibule seen in the crystal structure.

264 These results show how the periplasmic vestibule selectively recruits NH4(+) to the Am1 site, a

265 n contrast to forward translocation from the vestibule side of the pore, backward translocation times

266 n possibilities in pLGICs with an accessible vestibule site.

267 hK but makes different interactions with the vestibule, some of which are less favorable than for nat

268 ack of effect on HC-030031 inhibition by the vestibule substitutions suggests that this molecule inte

269 similarity in pore architecture and aqueous vestibules, suggesting that there are unanticipated yet

270 ain contains a large, moderately hydrophobic vestibule that can bind a substrate's transmembrane doma

271 xtracellular surface through a deep and wide vestibule that emanates from a narrower pathway between

272 is used to identify residues on hERG's outer vestibule that interact with specific residues on the in

273 cket for both modulators to an extracellular vestibule that overlaps with a region used by orthosteri

274 and without ammonia or methyl ammonia show a vestibule that recruits NH4+/NH3, a binding site for NH4

275 ptures the capacitive pile up of ions in the vestibules that link the bulk solution to the hydrophobi

276 Proximal to the exit port is a "folding vestibule" that permits the nascent peptide to compact a

277 ped hook region, colocated with the cochlear vestibule, that features the largest difference in fluid

278 In the end of this recruitment vestibule, the phenyl ring of Phe107 dynamically switche

279 sket folds allowed them to enter the protein vestibule, the propeller fold exceeds the size of the la

280 ns two basic residues into the extracellular vestibule to antagonize S4 gating-charge movement throug

281 and increases the accessibility of the inner vestibule to Cl(-) ions.

282 ngements that fully expose the intracellular vestibule to the cytoplasm.

283 s the solute transition from the periplasmic vestibule to the hydrophobic lumen in the Rh/Amt/MEP sup

284 the exposure of extracellular and cytosolic vestibules to the bulk phase was evaluated as the reacti

285 row constriction near their apex with a wide vestibule toward the cytoplasm and an aqueous central ca

286 TSET modification of a cysteine in the outer-vestibule turret (Kv2.1 position 356/Shaker position 425

287 ng the binding site toward the extracellular vestibule (Tyr-108 and Phe-253), and a salt bridge in th

288 ate that Glu66 and the prolines in the outer vestibule undergo large fluctuations, which are modulate

289 by EPR, we show that, on average, the outer vestibule undergoes a modest backbone conformational cha

290 se genetic fine-tuning of the biotin-binding vestibule, unrivaled levels of activity and selectivity

291 ing sites at the pore axis and extracellular vestibule, we propose a Ca(2+) permeation mechanism.

292 tion, the transmembrane pore and cytoplasmic vestibule were exceptionally wide.

293 is perched on top of the US28 extracellular vestibule, whereas its amino terminus projects into the

294 e channel protein and by ions present in the vestibules, whose dynamics are assessed using a flux con

295 utative nascent chain mimic, the cytoplasmic vestibule widens, and a lateral exit portal is opened th

296 al, potassium channels have an intramembrane vestibule with a selectivity filter situated above and a

297 polar pocket accessed from the extracellular vestibule with an important role for Asp-101.

298 d shape what has been described as a "closed vestibule," with their lateral portals obstructed by loo

299 The vestibule within the central pore of Na(+)-dependent cot

300 loop and occurs via an enclosed hydrophilic vestibule within the membrane formed by the subunits EMC