ライフサイエンスコーパス: virus assembly

1 large pool of mRNAs as virion genome during virus assembly.

2 matrix protein (NiV M) plays a major role in virus assembly.

3 an essential role of ORF45-ORF33 binding for virus assembly.

4 kely interacts with the cell membrane during virus assembly.

5 ma membrane represents an obligatory step of virus assembly.

6 e for immunosuppression but are required for virus assembly.

7 is packaging of the viral RNA genome during virus assembly.

8 0A and Y730A) were found to be defective for virus assembly.

9 for infectivity but does not affect in vivo virus assembly.

10 tis C virus particles, indicating a block in virus assembly.

11 of antiretroviral drugs that interfere with virus assembly.

12 ition to its previously demonstrated role in virus assembly.

13 is facilitated by YB-1, which promotes MMTV virus assembly.

14 ion of icosahedral viruses and regulation of virus assembly.

15 lso caused defects in RNA replication and/or virus assembly.

16 , HIV-1 must package viral RNA genome during virus assembly.

17 us virus release by 80-90% without affecting virus assembly.

18 to process virion precursor proteins used in virus assembly.

19 ation, post-replicative gene expression, and virus assembly.

20 be involved in the recruitment of the ER for virus assembly.

21 es of this fascinating and essential step in virus assembly.

22 ct on the entry process but greatly affected virus assembly.

23 ction, suggesting that they are required for virus assembly.

24 ich to describe scaffolding protein-mediated virus assembly.

25 al (Gag) and enzymatic (Pol) proteins during virus assembly.

26 e intracellular localization of pp150 during virus assembly.

27 cdE2 and Y162 and K252 of CP as critical for virus assembly.

28 for or regulates capsid shell closure during virus assembly.

29 tivity, of IVa2 was required for its role in virus assembly.

30 d binding to the plasma membrane (PM) during virus assembly.

31 yprotein plays several critical roles during virus assembly.

32 intracellular viral trafficking and progeny virus assembly.

33 ociation rates of the capsid proteins during virus assembly.

34 ovide further insights into the mechanism of virus assembly.

35 rotein may help stabilize M complexes during virus assembly.

36 droplets (LDs) that play a critical role in virus assembly.

37 cation and between NS3 and NS4A that control virus assembly.

38 s (CoV) E protein plays an important role in virus assembly.

39 is required for both genome replication and virus assembly.

40 ion and 25 had moderate-to-severe defects in virus assembly.

41 structural and nonstructural proteins during virus assembly.

42 oes dual processing events during infectious virus assembly.

43 HIV-1) Gag-RNA interactions are required for virus assembly.

44 revealing an as-yet-unknown role for NS3 in virus assembly.

45 s of vesicular stomatitis virus (VSV) during virus assembly.

46 hat it has been reported to be essential for virus assembly.

47 ar machinery that directs Gag to the site of virus assembly.

48 ,5-bisphosphate (PIP2) is critical for HIV-1 virus assembly.

49 laxity, protein B is absolutely required for virus assembly.

50 normally in the Golgi apparatus, the site of virus assembly.

51 is replication impaired and is defective in virus assembly.

52 ficked to the Golgi compartment, the site of virus assembly.

53 tail interacts with the nucleocapsid during virus assembly.

54 nce of observed RNase L activity until after virus assembly.

55 tes with the envelope protein complex during virus assembly.

56 ) tail addition is the rate-limiting step in virus assembly.

57 n that selects and packages HIV-1 RNA during virus assembly.

58 e key residues that appear to be involved in virus assembly.

59 undergoes a major conformational change upon virus assembly.

60 lements required for genome packaging during virus assembly.

61 t requirements are placed on the E2 tail for virus assembly.

62 that IHH support HCV genome replication and virus assembly.

63 ns at this compartment is a prerequisite for virus assembly.

64 y or tension within the protein shell during virus assembly.

65 e first direct evidence that VP22 influences virus assembly.

66 accumulates extensively in endosomes during virus assembly.

67 tailed studies into the mechanisms mediating virus assembly.

68 g (PrGag) proteins play an essential role in virus assembly.

69 both viral-genome replication and infectious-virus assembly.

70 pUL51 in promoting membrane scission during virus assembly.

71 egulation, RNA-protein interactions, and RNA virus assembly.

72 eraction with the M1 scaffold protein during virus assembly.

73 envelope C terminus, which is removed during virus assembly.

74 VSV nucleocapsids to the plasma membrane for virus assembly.

75 enome for initiation of DNA packaging during virus assembly.

76 t the PBS-segment of the HIV-1 5'-UTR during virus assembly.

77 ex is transported to the plasma membrane for virus assembly.

78 r trafficking, transcription activation, and virus assembly.

79 educe synthesis of other proteins needed for virus assembly.

80 ytoplasmic transport as an intrinsic step in virus assembly.

81 nv on the surface of infected cells prior to virus assembly.

82 c evidence implicates the helicase domain in virus assembly.

83 viral particles, likely through its role in virus assembly, a critical process for KSHV replication

84 nstraints on the length of the endodomain on virus assembly, amino acid insertions/substitutions at t

85 ) and examined the impact of MA mutations on virus assembly and ability to support spreading infectio

86 s-encoded tegument proteins are critical for virus assembly and are also, therefore, potentially attr

87 The nucleocapsid acts as a scaffold for virus assembly and as a template for genome transcriptio

88 Virus assembly and budding are critical but little under

89 that is spatially associated with polarized virus assembly and budding at sites of cell contact.

90 e protein and exploited them to characterize virus assembly and budding in living cells.

91 he plasma membrane, we hypothesized that the virus assembly and budding site determines the ability o

92 e HIV-1 matrix (MA) protein that altered the virus assembly and budding site to CD63(+)/Lamp-1-positi

93 he RSV matrix (M) protein plays key roles in virus assembly and budding, but the protein interactions

94 was a strong requirement for the E1 stem in virus assembly and budding, probably reflecting its impo

95 fect of the M2 cytoplasmic tail mutations on virus assembly and budding, we constructed a series of a

96 trix protein (M1) plays an important role in virus assembly and budding.

97 hannel for virus infection and also mediates virus assembly and budding.

98 viral matrix (M) proteins are key drivers of virus assembly and budding.

99 fts have been proposed to form platforms for virus assembly and budding.

100 and with the nucleocapsid (N) protein drive virus assembly and budding.

101 CA critical for Gag-Gag interactions during virus assembly and CA-CA interactions during core format

102 We propose that HIV-1 promotes virus assembly and cell-cell transfer in T cells by targ

103 res therefore reveal mechanisms of influenza virus assembly and disassembly.

104 roles and structures of the CVSC proteins in virus assembly and DNA packaging, we isolated a number o

105 s two copies of full-length viral RNA during virus assembly and efficient genome encapsidation is med

106 analyses and processing methods, details of virus assembly and egress at (sub)nanometer resolution w

107 We observed progressive stages of virus assembly and egress, including flower-like flat Ga

108 d capsid protein; thus, they may be sites of virus assembly and egress.

109 otein stability, proteolytic processing, and virus assembly and entry, these changes had minimal impa

110 on surface, is important in both influenza A virus assembly and entry.

111 f the PIV5 M protein is important for proper virus assembly and for the budding of infectious particl

112 report, we examined the role of HIV-1 RNA in virus assembly and found that packageable HIV-1 RNA enha

113 The effects of these mutations on virus assembly and function were determined in both vert

114 ese results underscore the complex nature of virus assembly and genome encapsidation and provide a ne

115 However, virus assembly and GPC incorporation into budded virions

116 by affecting HCV genesis through increasing virus assembly and HCV fitness by enhancing the virus sp

117 findings enhance our understanding of Ebola virus assembly and in so doing move us closer to the ide

118 s M1 and M2 proteins play important roles in virus assembly and in the morphology of virus particles.

119 ntial steps of phage genome movements during virus assembly and infection, are likely to be conserved

120 nderstanding the highly dynamic processes of virus assembly and infection.

121 erential functional mechanisms and roles for virus assembly and infection.

122 ture proteins and plays an important role in virus assembly and infection.

123 e RNA viruses, may direct activities such as virus assembly and innate immune modulation.

124 is challenging, but important to understand virus assembly and life cycle mechanisms that offer dist

125 Virus assembly and maturation proceed through the progra

126 During virus assembly and maturation, conformational switching

127 region of Pr55(Gag), which are important for virus assembly and maturation, were involved in the inte

128 unctional complex that is important for both virus assembly and modulation of host cell morphology.

129 lution cryo-EM for probing the mechanisms of virus assembly and morphogenesis.

130 ane fusion; during egress, HA contributes to virus assembly and morphology.

131 se in the cytoplasm in the time required for virus assembly and must be transported by cytoskeletal e

132 ch provides interesting insights about giant virus assembly and paves the way for vaccine development

133 rocess can be either antiviral, by affecting virus assembly and production, or beneficial for the vir

134 Hepatitis C virus assembly and release depend on viral interactions

135 in HPS2 cells did not reverse the inhibited virus assembly and release imposed by the AP-3 deficienc

136 el fits to the data suggest that the rate of virus assembly and release is limited by host cell resou

137 derstanding the role of the CoV E protein in virus assembly and release is thus an important prerequi

138 e mutations also adversely affect infectious virus assembly and release, processes in which NS3 also

139 it in HPS2 fibroblasts restored the impaired virus assembly and release.

140 models that include HCV RNA secretion and/or virus assembly and release.

141 tion to the previously reported functions in virus assembly and spread for pUL51, the pUL7-pUL51 comp

142 protein E and functions at multiple steps in virus assembly and spread in epithelial cells.

143 RNA has been postulated as a major force in virus assembly and stabilization.

144 elp define protein interactions critical for virus assembly and suggest a fundamental difference betw

145 The beta-turn is critical for immature virus assembly and the 6-helix bundle regulates proteoly

146 that first localizes to the cellular site of virus assembly and then inserts into the virion envelope

147 coat protein subunit, which is essential for virus assembly and which was missing from previously det

148 n in the regulation of RNA amplification and virus assembly and, finally, a viral protease suppressin

149 tion for enhanced gene expression and in the virus assembly and/or budding, which are required for th

150 vesicular structures, indicating a defect in virus assembly and/or budding.

151 ell as between NS2 and NS3 are essential for virus assembly and/or release and that each of these vir

152 indicate that hnRNP K is likely involved in virus assembly and/or release from infected cells.

153 s Golgi network membrane-associated roles in virus assembly and/or release through a viroporin-like a

154 types at sites of interactions essential for virus assembly and/or release.

155 For many viruses, assembly and budding occur simultaneously durin

156 dosomes are sites for human immunodeficiency virus assembly, and increasing ART concentrations in suc

157 Gag polyprotein is a critical determinant of virus assembly, and is therefore a potential target for

158 S5A protein has roles in genome replication, virus assembly, and modulation of host pathways.

159 's top binding site is maintained throughout virus assembly, and specifically binds and assembles wit

160 , substitutions affecting host cell tropism, virus assembly, and the ability to inhibit cellular anti

161 at E3 has an enzymatic or functional role in virus assembly, and these possibilities are further disc

162 e possible roles played by aggresomes during virus assembly are emerging from an understanding of how

163 els have been developed that treat spherical virus assembly as an equilibrium process.

164 cles from cells in a plasmid-based influenza virus assembly assay, and hemagglutinating material from

165 ques to describe unique aspects of influenza virus assembly, biochemical techniques to study viral ge

166 s package two copies of their genomes during virus assembly, both of which are required for strand tr

167 wn to partially restore H348/352A growth and virus assembly/budding, while neither rescued the decrea

168 different rearrangement, is not involved in virus assembly but instead uniquely binds RNA to regulat

169 n of the VSV M protein plays a minor role in virus assembly but is involved in virus-host interaction

170 ition of SFK activity did not interfere with virus assembly but prevented transit of virions through

171 te that the MA(NOS) mutation does not affect virus assembly, but does prevent virus spread, in feline

172 Abrogation of virus assembly by a single-amino-acid change bodes well

173 functions as a scaffold that helps initiate virus assembly by exposing a cluster of conserved UCUG e

174 ons is matrix protein 1 (M1), which mediates virus assembly by forming an endoskeleton beneath the vi

175 negatively charged residues are involved in virus assembly by mediating interaction between the memb

176 results suggest that NS2 acts to coordinate virus assembly by mediating interactions between envelop

177 (MA) domain of HIV-1 Gag plays key roles in virus assembly by targeting the Gag precursor to the pla

178 among Gag multimerization, membrane binding, virus assembly, CA dimerization, particle maturation, an

179 calization of the viral protein pp150 to the virus assembly compartment (AC) is dependent on its dire

180 irus failed to efficiently accumulate in the virus assembly compartment (AC).

181 Clathrin accumulated in the cytoplasmic virus assembly compartment (vAC) of infected cells co-lo

182 lar localization of the gM/gN protein to the virus assembly compartment compared to the wild-type pro

183 capsids (light particles) were seen in large virus assembly compartments near the disorganized Golgi

184 thereby preventing the correct formation of virus assembly compartments.IMPORTANCE This study of VZV

185 s HCV production by enhancing NS2-associated virus assembly complex formation near LD.

186 Furthermore, the virus assembly defect of NS4A K41A was suppressed by NS3

187 on previously implicated in overcoming other virus assembly defects.

188 quirements of the dimeric capsid protein for virus assembly/disassembly have not been characterized.

189 During virus assembly, genome packaging involves the delivery o

190 Virus assembly has not been routinely targeted in the de

191 sm by which Env proteins are acquired during virus assembly has yet to be fully defined.

192 s of NS2 involved in HCV RNA replication and virus assembly, i.e., NS2-NS3 autoprocessing and E2 recr

193 is required for the successful completion of virus assembly, (iii) several of the protein components

194 lementary roles of the M1 and M2 proteins in virus assembly.IMPORTANCE Influenza virus particle assem

195 1 achieves efficient genome packaging during virus assembly.IMPORTANCE Retrovirus assembly is a well-

196 ing the protein as important for influenza A virus assembly in addition to its well-documented role d

197 reporters during plasmid-directed influenza virus assembly in cells, we have now mapped cis-acting s

198 It has previously been demonstrated that virus assembly in macrophages occurs in cytoplasmic vesi

199 est a fundamental difference between Sindbis virus assembly in mammalian and insect cells.

200 (E2 DeltaK391) resulted in the disruption of virus assembly in mammalian cells but not insect cells (

201 scence assay to monitor RNA conformation and virus assembly in real time, with two viruses from diffe

202 mportant roles in cell-to-cell spread and in virus assembly in the cytoplasm, both of which likely de

203 Analysis of the reaction suggests that (i) virus assembly in vitro is optimal under conditions that

204 A are necessary and sufficient for efficient virus assembly in vitro.

205 These results suggest a new model of virus assembly in which an interaction of VSV nucleocaps

206 can block NS3 functions in RNA synthesis and virus assembly, in addition to inhibiting polyprotein pr

207 re and biochemical properties of this unique virus assembly intermediate will provide new insights in

208 Influenza A virus assembly is a complex process that requires the in

209 Once virus assembly is complete, retroviruses, like most enve

210 a packageable viral RNA is not required for virus assembly is currently unknown.

211 lective packaging of the HIV-1 genome during virus assembly is mediated by interactions between the d

212 Gag protein on the plasma membrane (PM) for virus assembly is mediated by specific interactions betw

213 We establish that virus assembly is sensitive to mutations in the linker r

214 Paramyxovirus matrix (M) proteins organize virus assembly, linking viral glycoproteins and viral ri

215 RNA synthesis and also block a late stage in virus assembly/maturation at clinically relevant concent

216 esults show that activating and deregulating virus assembly may be a powerful general approach for an

217 NA switch mechanism and further suggest that virus assembly may be initiated by a complex comprising

218 Proteins packaged during virus assembly may subsequently form the first line of a

219 To gain insights into the RNA packaging and virus assembly mechanisms, we labeled and monitored the

220 road biological implications with respect to virus assembly mechanisms.

221 s) have been shown to play a pivotal role in virus assembly, morphogenesis, and infection of host cel

222 y of the role of individual AHSV proteins in virus assembly, morphogenesis, and pathogenesis.

223 vity late during the course of infection, as virus assembly nears completion.

224 between the phase of the cell cycle at which virus assembly occurred and histone modifications in the

225 ure retroviruses, in order to understand how virus assembly occurs, and how maturation takes place.

226 late RAB27A, which is required for enveloped virus assembly of human cytomegalovirus.

227 Virus replication and virus assembly often occur in virus inclusions or virus

228 ons on a coarse-grained model that describes virus assembly on a fluctuating lipid membrane.

229 T lymphocytes, while intracellular sites of virus assembly or accumulation are apparent in macrophag

230 nt intermediates predicted by simulations of virus assembly or disassembly.

231 nd of itself has no effect on the process of virus assembly or on the ability of virus to infect cell

232 ubparticles formed during normal or aberrant virus assembly (or as a result of damage to the intact a

233 following virus entry, during encapsidation/virus assembly, or within the nucleus may reflect virus

234 important role of the glycoprotein gM during virus assembly, particularly in the dynamics of gM traff

235 diverse functions at different stages in the virus assembly pathway will require more detailed inform

236 n whether it may occur transiently along the virus assembly pathway.

237 virus (RSV) Gag protein is intrinsic to the virus assembly pathway.

238 lear trafficking as an intrinsic part of the virus assembly pathway.

239 s indicate that phosphorylation of L2 during virus assembly plays an important role in optimal uncoat

240 s encode matrix proteins that coordinate the virus assembly process.

241 l rearrangement during the membrane-mediated virus assembly process.

242 zation often occurs at an early stage of the virus assembly process.

243 and with viral arrays and other features of virus assembly, provide evidence of a productive direct

244 here for the first time that RSV M's role in virus assembly/release is strongly dependent on threonin

245 is, but it inhibited syncytium formation and virus assembly/release.

246 rget to inhibit both HCV RNA replication and virus assembly, representing a new avenue for host-targe

247 Icosahedral virus assembly requires a series of concerted and highly

248 Dengue virus assembly requires cleavage of viral C-prM-E polypr

249 ion in virus yield was related to a block in virus assembly, since in the presence of either BFA or G

250 accumulation of SARS CoV S protein near the virus assembly site for interaction with other viral str

251 to be important for concentrating S near the virus assembly site rather than for direct interaction w

252 proteins and the viral ribonucleoproteins at virus assembly sites and often recruit host machinery th

253 nd RNAs, helps transport Pr55Gag proteins to virus assembly sites at the plasma membranes of infected

254 by recruiting envelope protein 2 (E2) to the virus assembly sites located at the detergent-resistant

255 hibits both NS2 localization to the putative virus assembly sites near lipid droplets (LD) and NS2 in

256 es p7-dependent NS2 localization to putative virus assembly sites near lipid droplets (LD).

257 ins and viral ribonucleoproteins together at virus assembly sites on cellular membranes.

258 that retention of Env trimers within single virus assembly sites requires the Env cytoplasmic tail (

259 he recruitment of Tsg101 and other ESCRTs to virus assembly sites where they mediate budding.

260 omote the association of these proteins with virus assembly sites within the plasma membrane.

261 and HCV envelope protein recruitment to the virus assembly sites, which in turn promote HCV RNA repl

262 brane and recruits envelope glycoproteins to virus assembly sites.

263 istent with its role in recruiting E2 to the virus assembly sites.

264 To determine whether L9 plays a role in virus assembly, small interfering RNA (siRNA)-mediated k

265 I and active-site PI were both able to block virus assembly soon (<12 h) after drug treatment, sugges

266 In contrast, virus assembly studies indicate that the MA(NOS) mutatio

267 TCV) is a multifunctional protein needed for virus assembly, suppression of RNA silencing-based antiv

268 However, a unique in vitro cell-free virus assembly system was subsequently developed, showin

269 In one-scaffolding-protein virus assembly systems, coat proteins promiscuously inte

270 its trafficking to the plasma membrane where virus assembly takes place.

271 ue a defect in an early-intermediate step in virus assembly that follows the recruitment of NS5A to l

272 step after genome replication but preceding virus assembly that is dependent on 3CD and/or 3AB prote

273 nd can identify small-molecule inhibitors of virus assembly that prevent, inappropriately accelerate

274 During virus assembly, the gp120/gp41 complex is incorporated a

275 y minor effects of the M protein mutation on virus assembly, the mutant virus exhibited growth restri

276 n of EV71, including a putative precursor in virus assembly, the procapsid, and the mature virus caps

277 Our model suggests that during virus assembly, the trimer of E1/E2 may be further assem

278 tifs that recruit host machinery to sites of virus assembly, thereby promoting particle release from

279 irus membrane (M) proteins play key roles in virus assembly, through M-M, M-spike (S), and M-nucleoca

280 e deaminases that can be encapsidated during virus assembly to catalyze C-->U deamination of the vira

281 of the virus replication cycle, ranging from virus assembly to cell-cell spread of the virus, and hen

282 omains, thereby coupling Gag recruitment and virus assembly to Env accumulation at the cell-cell inte

283 NCE HIV-1 must package its RNA genome during virus assembly to generate infectious viruses.

284 nd vRNA to the plasma membrane for efficient virus assembly to occur.

285 results show that for respiratory syncytial virus assembly, viral filaments are produced and loaded

286 y, a stochastic model of single-stranded RNA virus assembly was created to model the cooperative effe

287 ein, indicating that this function of NS3 in virus assembly was independent of its known enzymatic fu

288 However, mutant virus assembly was reduced in parallel with reduced viru

289 The role of the M protein in virus assembly was then examined by infecting HEp-2 and

290 By examining physical models of spherical virus assembly we have arrived at a general synthetic st

291 the trafficking of the gM/gN complex during virus assembly, we made a series of gM (UL100 open readi

292 al evidence that morphogenesis of the AC and virus assembly were dynein dependent.

293 sly identified to play a role in influenza A virus assembly were found to complement the lethal M2Y76

294 nometer (<10-A) resolution of an icosahedral virus assembly were obtained by cryogenic electron micro

295 mportantly, several mutations that inhibited virus assembly were shown to inhibit NS2 protein complex

296 itates IBDV replication complex function and virus assembly, which is critical to completion of the v

297 the M1 proteins in their ability to support virus assembly with nonpalmitoylated H3 HA.

298 ut also specifically inhibited intracellular virus assembly without affecting HCV RNA replication.

299 exclude tetherin from the specific sites of virus assembly without overtly removing it from the cell

300 and Chk2, are mislocalized to a cytoplasmic virus assembly zone, where they are colocalized with vir