ライフサイエンスコーパス: vitrify

1 d within interstitial microfluids until they vitrify.

2 g that it is physically possible to thin and vitrify a specimen for electron cryomicroscopy (cryoEM)

3 patients with POR, with fewer than 5 oocytes vitrified across all ovarian stimulation cycles.

4 Vitrified Affinity Grid specimens could be used to calcu

5 e monolayer-purified Tf-TfR samples could be vitrified and used to calculate a 3D reconstruction of t

6 ed in 1), freeze-dried, 2), frozen-hydrated (vitrified), and 3), initially alive states.

7 e behavior and internal structure of liquid, vitrified, and crystallized water-salt ultrafine aerosol

8 Embryos were vitrified, and then one or two day-3 embryos or one day-

9 Sugars vitrifying around fluid phase lipids lowered T(m) below

10 Sugars vitrifying around gel phase lipids raised T(m) during th

11 M22 and EG+sucrose vitrified at 0.5 L, but only M22 succeeded at 3 L; VS55

12 in, and 68.6% taurocholate (all mole %) were vitrified at 2 min to 20 days after fourfold dilution to

13 archaeal ketol-acid reductoisomerase (KARI) vitrified at 4-70 degrees C, and show that structures of

14 ty of solving cryo-EM structures of proteins vitrified at high temperatures, solve 12 structures of a

15 l TEM can only be used to image air-dried or vitrified bacteria removed from their natural environmen

16 The cells are then screened by fLM and vitrified before acquisition of cryo-fLM and cryo-ET ima

17 n cell-containing droplets which may then be vitrified before being exposed to high temperatures for

18 onded organic framework (HOF) that melts and vitrifies below 100 degrees C.

19 Imaging of fully hydrated, vitrified biological samples by electron tomography yiel

20 vantages of a technique that directly images vitrified biomolecules without the need for crystallizat

21 Taken together, HHP promotes competence of vitrified bovine blastocysts through modest transcriptio

22 nsional crystals of aquaporin-1 preserved in vitrified buffer in the absence of any additive.

23 Dextran and polyvinylpyrrolidone (PVP) also vitrified but did not depress T(m) during drying.

24 composition will be chemically processed and vitrified, but the varying chemical composition and high

25 bon-coated electron microscopy grids and are vitrified by plunging them into liquid ethane.

26 by cryo-electron tomography (cryo-ET) of re-vitrified cell sections prepared using the Tokuyasu meth

27 ow for the subcellular analyses of unstained vitrified cells and three-dimensional localization of sp

28 eparation of thin (200-500 nm) lamellae from vitrified cells grown on electron microscopy (EM) grids.

29 ntly developed methods for cryosectioning of vitrified cells.

30 One way to avoid it is to vitrify cells; that is, to convert cell water to a glass

31 employed to guide the FIB milling process of vitrified cellular samples and to capture specific struc

32 the native structure of the COPI coat within vitrified Chlamydomonas reinhardtii cells.

33 We find that the vitrifying diluent does not adversely affect device perf

34 Additionally, some of the considered alloys vitrify during sputtering, which also allows us to study

35 to the preservation of glassy walls called 'vitrified forts'.

36 In vitrified frozen-hydrated mammalian cells overexpressing

37 s, together with rapid cooling, results in a vitrified, highly viscous state in which cells can remai

38 ids archaeological forensic investigation of vitrified hillforts and interpretation of source rock ma

39 European Bronze and Iron Age vitrified hillforts have been known since the 1700s, but

40 on sources can produce cryogenic lamellae of vitrified human cells in a robust and automated manner,

41 Preliminary morphologic results with vitrified human cornea also showed retention of endothel

42 stributions of native spike conformations on vitrified human coronavirus NL63 (HCoV-NL63) virions wit

43 lectron tomograms of ternary membranes under vitrified, hydrated conditions.

44 ive imaging of submicron membrane domains in vitrified, hydrated vesicles.

45 Vitrified-hydrated virions were found to be spherical, w

46 o-EM grids-aimed at preserving thin, uniform vitrified ice and improving protein adsorption-have been

47 phene grid allows the formation of ultrathin vitrified ice layer embedded most protein particles at t

48 Particles are equilibrated at a fixed RH, vitrified in a temperature-controlled flow tube, and ima

49 d the mixing reaction stops when the grid is vitrified in liquid ethane ~100 ms later.

50 nfluenced the degree to which buildings were vitrified in the Iron Age.

51 molecular structures of the SPMTs and CFs in vitrified intact and detergent-extracted parasites.

52 conjectured that any metallic liquid can be vitrified into a glassy state provided that the cooling

53 ses rapidly until at the glass transition it vitrifies into an amorphous solid accompanied by a steep

54 We show that vitrified kidneys can be cryogenically stored (up to 100

55 In this work, we vitrify liquid droplets through cooling at 10(6) K . s(-

56 The vitrified medium renders stationary emulsion droplets th

57 n a cryo-electron microscopy study of intact vitrified mouse embryonic cells, and in an ultrastructur

58 trification could improve the development of vitrified mouse oocyte.

59 ous solutions of anhydrobiotic trehalose are vitrified on arbitrary substrates, producing glassy coat

60 ences in the oocyte survival among fresh and vitrified oocyte; however, the addition of ffEVs to vitr

61 Vitrified oocytes were thawed with or without ffEVs, ass

62 bly low rate of return to utilize previously vitrified oocytes; notably, patients with POR are more l

63 s suppressed, and the POSS-MOP mixtures will vitrify or jam as a function of decreasing temperature.

64 Vitrified, or "frozen", donor eggs can either be fertili

65 However, rewarming of vitrified organs can similarly fail due to ice crystalli

66 plunge-freezing device (the FEI Vitrobot) to vitrify our samples, we discuss its operation and parame

67 levels and lower mitochondrial activity than vitrified ovarian tissue.

68 ompared with those for rock samples from the vitrified part of the wall, as well as with equilibrium

69 large number of damaged mitochondria, while vitrified PG oocytes had intact mitochondria and cell pl

70 iation-based X-ray fluorescence (SR-XRF), on vitrified polymorphonuclear neutrophils (PMNs) during th

71 cepted explanation for why liquids sometimes vitrify rather than crystallize remains hotly pursued, d

72 When applied to vitrified samples, serial FIB/SEM is also a means to tar

73 etermined in three dimensions from images of vitrified samples.

74 Cryo-electron tomography of the vitrified sections revealed the prion assemblies as alig

75 omposition and the form of the pyrolyzed and vitrified sludge were analyzed by scanning electron micr

76 cooling, the phase transition from liquid to vitrified solid (i.e., vitrification) and the levitation

77 ow-density amorphous ice in coexistence with vitrified solute rich aqueous glass.

78 ructure of proteins and protein complexes in vitrified solution.

79 Angular reconstitution from images of vitrified specimens does not reliably converge on the co

80 erved and tomographic reconstructions of the vitrified specimens indicated adhesion to the interfaces

81 Alignment of class averages from vitrified specimens to a 3D negative stain reference mod

82 de cells and tissues require methods to thin vitrified specimens to electron transparency.

83 l array cryo-tomography (MPACT) tailored for vitrified specimens.

84 cryo-FM, while maintaining the specimen in a vitrified state for subsequent cryo-EM.

85 solids (vitrify) upon desiccation, and this vitrified state mirrors their protective capabilities.

86 matically as a function of conversion in the vitrified state, and the utilization of soft-start or pu

87 ndering phases that were locked into glassy, vitrified states.

88 d, namely S2 = r(eff)o/rTo, where rTo is the vitrified steady-state excitation anisotropy, we recover

89 vidence supports that LEA proteins stabilize vitrified sugar glasses thought to be important in the d

90 Although structures of vitrified supramolecular complexes have been determined

91 d by thermal plasma, a process which aims to vitrify the galvanic sludge and render metals (iron, zin

92 We vitrify the tissue via plunge-freezing and use xenon pla

93 , amorphous solids always become softer when vitrifying the melts under higher cooling rates.

94 quid tantalum and vanadium, are successfully vitrified to form metallic glasses suitable for property

95 TDPs form non-crystalline amorphous solids (vitrify) upon desiccation, and this vitrified state mirr

96 volume; ~0.23-0.75 L organ volume) were also vitrified using EG+sucrose, though not rewarmed.

97 After, blastocysts were vitrified using OPS.

98 ndergo in vitro maturation, cat oocytes were vitrified using the Cryotop method in the presence or ab

99 In vivo studies of vitrified vessel segments in an autologous transplant mo

100 erved in the elastic properties of fresh and vitrified vessels (P>0.05 for each of DC, Ep, and beta).

101 tion in the wall viscosity between fresh and vitrified vessels appeared to be nonsignificant (theta=1

102 The maximum contractions achieved in vitrified vessels were >80% of fresh matched controls wi

103 The vitrified-warmed blastocysts (IVF + VT group) exhibited

104 blastocyst vitrification on the phenotype of vitrified-warmed blastocysts, their intrauterine and pos

105 epatic gene expression in the offspring from vitrified-warmed blastocysts.

106 Histological evaluation of the vitrified-warmed tissue showed large numbers of degenera

107 is revealed that having fewer than 5 oocytes vitrified was associated with higher odds of utilizing o

108 action of amyloid fibrils in a thin layer of vitrified water at cryogenic temperatures, we were able

109 Vitrified water shows all features that have been report

110 (BMG) formers are multicomponent alloys that vitrify with remarkable ease during solidification.