ライフサイエンスコーパス: water permeability

1 d NHDF-Ad fibroblasts (aquaporin-facilitated water permeability).

2 pids in hair does not lead to an increase of water permeability.

3 s to a clear and selective increase in their water permeability.

4 ffecting AQP4 expression, array assembly, or water permeability.

5 may explain why the peptide seems to affect water permeability.

6 QP4 SNPs and showed that four nsSNPs reduced water permeability.

7 nd had approximately 12-fold reduced osmotic water permeability.

8 d calmodulin has been shown to regulate AQP0 water permeability.

9 volvement of AQPs 3, 4, and 5 in high airway water permeability.

10 s expected, they had reduced plasma membrane water permeability.

11 endothelia, where it increases cell membrane water permeability.

12 small molecules for inhibition of mouse AQP3 water permeability.

13 y reduced [Arg8]vasopressin (AVP)-stimulated water permeability.

14 uaporins (AQPs) are important in controlling water permeability.

15 ether, despite an up to 30-fold reduction in water permeability.

16 Isolated ZGs exhibit low basal water permeability.

17 , apical exocytosis, and increase of osmotic water permeability.

18 QP1-null mice despite a 20-fold reduction in water permeability.

19 estigated to better understand their role in water permeability.

20 or about 15% of total cholangiocyte membrane water permeability.

21 amino acid in facilitating the modulation of water permeability.

22 diates vasopressin-regulated collecting duct water permeability.

23 Ildr1 significantly reduces the paracellular water permeability.

24 ght into the molecular mechanism of membrane water permeability.

25 s can impact GO membrane characteristics and water permeability.

26 by a pollen-specific CPK that modifies their water permeability.

27 ere also associated with an elevated osmotic water permeability.

28 among the laminated GO nanosheets for higher water permeability.

29 h the means to rapidly and reversibly modify water permeability.

30 ins in solute rejection with lower losses in water permeability.

31 d to the partial restoration of the membrane water permeability.

32 the molecular regulation of collecting duct water permeability.

33 iposomes up to 1:200 increased their osmotic water permeability.

34 0 S/m), good NaCl rejection (>95%), and high water permeability.

35 iple factors have been found to affect their water permeability.

36 th-matching membrane mimetics could speed up water permeability.

37 ions of the two polypeptides exhibit similar water permeabilities.

38 Lowering Ca(2+) increased water permeability 4.1 +/- 0.4-fold.

39 Diethylpyrocarbonate pretreatment increased water permeability 4.2 +/- 0.5-fold and abolished pH sen

40 nly used FO membranes were characterized for water permeability ( A), solute permeability ( B), and s

41 cutoff of 255 Da, and a reasonably high pure water permeability (A) of 2.4 LMH/bar.

42 Regulation of AQP0 water permeability, a well-studied phenomenon in vitro,

43 ovel method for measuring osmotically driven water permeability across microvessels in intact lung.

44 To measure the osmotic water permeability across the microvascular endothelial

45 The increased water permeability after birth may facilitate the mainte

46 etion resulted in a > 4-fold reduced osmotic water permeability and > 2-fold reduced glycerol permeab

47 We reported increased water permeability and a low urea reflection coefficient

48 Here, we have characterized water permeability and amiloride effects in well differe

49 uaporin 5), which is required for regulating water permeability and cell volume.

50 in decreased transepithelial proximal tubule water permeability and defective fluid absorption.

51 AQP-1 enhances osmotic water permeability and FGF-induced dynamic membrane bleb

52 1 play a role in decreasing collecting duct water permeability and increasing water excretion, where

53 a membrane blebs, where it increases osmotic water permeability and locally facilitates the rapid, tr

54 posomes were found to have excellent osmotic water permeability and NaCl reflection coefficient value

55 o (PLR), and the addition of cholesterol) on water permeability and NaCl retention.

56 on of NF-kappaB prevented the changes in the water permeability and reflection coefficient to protein

57 The water permeability and regulation characteristics of eac

58 ation experiments revealed increases in both water permeability and salt passage as a result of oxida

59 Besides, the water permeability and salt rejection of the thermally r

60 udy the roles of aquaporin (AQP) in cellular water permeability and screen AQP-specific drugs.

61 O) and nanofiltration (NF) due to their high water permeability and selectivity.

62 Their high water permeability and solute rejection make them potent

63 e direct relevance to regulation of membrane water permeability and water homeostasis in epithelia of

64 red in spheroids of T47D breast cells (basal water permeability) and NHDF-Ad fibroblasts (aquaporin-f

65 reconstitution despite a 100-fold increased water permeability, and (c) CO2 blow-off by the lung in

66 In oocytes, AQP1-AQP1 exhibited high osmotic water permeability, and AQP1-C189M exhibited half activi

67 annels have been designed to mimic this high water permeability, and none reject ions at a significan

68 transparency, we compared corneal thickness, water permeability, and response to experimental swellin

69 secretory vesicles, AQP1-AQP1 exhibited high water permeability, AQP1-C189M exhibited half activity,

70 ross lipid bilayer membranes with a relative water permeability as high as 17 mum s(-1).

71 c for LRRC8A with patch-clamp analyses and a water-permeability assay.

72 Water permeability assays in the cells were used to meas

73 Water permeability assays showed a high Pf in cells expr

74 re developed to quantify membrane and tissue water permeabilities at the ocular surface and to compar

75 ic environment, the apparent coefficient for water permeability at 21 degrees C varied modestly in a

76 s of varying composition gave widely varying water permeabilities but similar CO2 permeabilities at 2

77 of the 34 kDa polypeptide does not influence water permeability but may contribute to membrane traffi

78 f these mutant channels showed a decrease in water permeability but not the expected increase in glyc

79 In constrast, AtNIP6;1 showed no measurable water permeability but transported glycerol, formamide,

80 AQP4 into proteoliposomes increased osmotic water permeability by >40-fold, giving a p(f) of 15 x 10

81 ositive control, 0.3 mM HgCl2 inhibited AQP1 water permeability by >95%.

82 but increased their plasma membrane osmotic water permeability by 5- to 10-fold.

83 These results suggest that TEA reduces AQP1 water permeability by interacting with loop E.

84 own right and discuss how regulation of its water permeability by pH and calcium came to be discover

85 role in regulation of renal collecting duct water permeability by vasopressin.

86 le for non-muscle myosin II in regulation of water permeability by vasopressin.

87 ese results indicate that corneal epithelial water permeability can be regulated, presumably to prote

88 The high water permeability characteristic of mammalian red cell

89 a secretory agonist (forskolin), the osmotic water permeability coefficient (P(f)) decreasing up to 5

90 model was developed to determine the osmotic water permeability coefficient (Pf) of lung microvessels

91 osmotic challenges and estimated the osmotic water permeability coefficient, P(f), to be approximatel

92 orm a water-filled beta-sandwich that induce water permeability comparable with channel-forming prote

93 It displayed approximately 10% average water permeability compared to WT AqpZ, which had been a

94 led with CAII demonstrated increased osmotic water permeability compared with ghosts resealed with al

95 and CF airway epithelia have relatively high water permeabilities, consistent with the isosmotic ASL

96 AQP0 reduces its osmotic water permeability constant (Pf) in response to increase

97 Expression of AQP1 and osmotic water permeability (control P(f) = 0.046 +/- 0.005 cm/se

98 Water permeability decreased by 65% in parotid and 77% i

99 extremely tortuous (tortuosity ~10(3)), with water permeability decreasing from 20 to <1 L m(-2) h(-1

100 sorption in distal nephron segments with low water permeability, diuretic agents that impair this mec

101 hat mediate the fine-tuning of cell membrane water permeability during development or in response to

102 s or in AQP5-expressing oocytes with osmotic water permeabilities equivalent to those seen with AQP1.

103 se results indicate very different intrinsic water permeabilities for the mammalian aquaporin homolog

104 ese nanochannels nearly tripled the membrane water permeability for the optimal membrane.

105 stribution of channel water and an increased water permeability for the W48F/F200T mutant.

106 The low water permeability found in AQP1/UT-B null erythrocytes

107 putative D. discoideum AQPs, WacA, and AqpA, water permeability has not been shown.

108 expressing aquaporins have high or regulated water permeability; however, there has been no direct ev

109 opus laevis oocytes, AQP6 exhibits low basal water permeability; however, when treated with the known

110 tent and efficacious inhibitor of mouse AQP7 water permeability (IC(50), ~0.2 mum).

111 We report that water permeability in 0.8-nanometer-diameter carbon nano

112 AQP5 is the major pathway for regulating the water permeability in acinar cells, a critical property

113 Water permeability in an AQP4-enriched brain vesicle fra

114 xplain physiological observations that found water permeability in AQP0 to be substantially lower tha

115 Whereas osmotic water permeability in AQP1-reconstituted liposomes was >

116 Reduction in water permeability in chitosan-conditioned dentin, attri

117 enic diabetes insipidus produced by impaired water permeability in collecting-duct basolateral membra

118 res, supporting the involvement of aquaporin water permeability in cytokine secretion.

119 We found reduced osmotic water permeability in freshly isolated DRG neurons from

120 rted here to measure osmotic and diffusional water permeability in intact airways.

121 Herein we map the water permeability in intact arteries, using coherent an

122 We developed novel methods to compare water permeability in isolated choroid plexus of wild-ty

123 AQP1 deficiency reduced plasma membrane water permeability in lens epithelium by 2.8 +/- 0.3-fol

124 Aquaporins increase the water permeability in many cell types across many specie

125 ds indicated that amiloride does not inhibit water permeability in non-cystic fibrosis (non-CF) or CF

126 rane and led to a significant enhancement of water permeability in oocytes.

127 In contrast, the water permeability in parotid and sublingual acinar cell

128 results provide the first functional data on water permeability in perinatal lung.

129 Thus, the high water permeability in proximal tubule of wild-type mice

130 cell surface expression of AQP2 and osmotic water permeability in the absence of forskolin stimulati

131 Vasopressin antagonists, which promote low water permeability in the collecting ducts and, hence, f

132 The molecular determinants of water permeability in the human lung are incompletely de

133 chanism is sufficient to explain the reduced water permeability in the mutant without invoking effect

134 reases in vasopressin (AVP)-elicited osmotic water permeability in the terminal inner medullary colle

135 It has been controversial whether high water permeability in the thin descending limb of Henle

136 oM significantly inhibited or increased AQP1 water permeability in these assays.

137 er transport, we measured osmotically driven water permeability in vitro in microperfused OMDVR from

138 on decreased membrane AQP2 and AQP2-mediated water permeability in Xenopus oocytes expressing P2X(2),

139 g measurements indicated erythrocyte osmotic water permeabilities (in cm/s x 0.01, 10 degrees C): 2.1

140 crease in proteinuria, as well as glomerular water permeability, in the context of progressive deplet

141 Because the AQP4 M1 isoform exhibits greater water permeability, in this study, we explored the possi

142 quaporin-2 trafficking and forskolin-induced water permeability increase were blocked by F-actin disr

143 lation of apical aquaporin-2 trafficking and water permeability increase.

144 , the prominent changes in lysis tension and water permeability indicate that major changes occur in

145 None of the above altered the water permeability induced by AQP1 or AQP4.

146 Because the greatest change in AQP0 water permeability is in the normal pH range found in th

147 Water permeability is necessary but also insufficient.

148 equilibration, even when intrinsic membrane water permeability is not high.

149 AQP5-null mice indicates that high alveolar water permeability is not required for active, near-isos

150 tion of our present understanding of how its water permeability is regulated by pH and calcium.

151 evidence that high epithelial cell membrane water permeability is required for active, near-isosmola

152 beled dextran, an in vivo marker of membrane water permeability, is trapped in the basal infoldings o

153 Passive water permeabilities (Lp) of the cotransporters were det

154 Water permeability measured between the airspace and vas

155 Microvascular endothelial water permeability, measured by a related pleural surfac

156 Osmotic water permeability, measured by stopped-flow light scatt

157 Water permeability measurements indicated that functiona

158 er, we improved the signal-to-noise ratio of water permeability measurements on AQP0 proteoliposomes.

159 e plasma membrane and abolished the membrane water permeability mediated by transgenic PIP2;7.

160 reproduce and predict volume changes in high-water-permeability membranes under hypoosmotic gradients

161 The channel (unit) water permeabilities of AQP4 measured by osmotic-gradien

162 ocytes expressing AQP2 cRNAs, single-channel water permeabilities of mutants L22V, T126M, and A147T w

163 The gas and water permeabilities of these nanotube-based membranes a

164 Here we test the hypothesis that the water permeabilities of two naturally occurring isoforms

165 rojected to achieve <92% NaCl rejection at a water permeability of <1 L m(-2) h(-1) bar(-1), even wit

166 llow fiber membrane exhibits an average pure water permeability of 3.2 L m(-2) h(-1) bar(-1) and show

167 wly developed membrane exhibited a high pure water permeability of 5.01 L m(-2) h(-1) bar(-1) and com

168 a fast time constant associated with osmotic water permeability of AQP-expressing cells and a slow ph

169 In Xenopus oocytes, the water permeability of AQP0 (P(f)) increases with removal

170 e additive effects and together increase the water permeability of AQP0 40-fold to a level comparable

171 d into liposomes are challenging because the water permeability of AQP0 is only slightly higher than

172 Our analysis suggests that the low water permeability of AQP0 may help maintain the mechani

173 nt environment, both may act to increase the water permeability of AQP0.

174 Previous experiments have shown that the water permeability of AQP4 depends on the cholesterol co

175 the known water channel inhibitor, Hg2+, the water permeability of AQP6 oocytes rapidly rises up to t

176 xtraordinary long intracellular loop induced water permeability of AqpB, hinting at a novel gating me

177 Lowering pH to 6.5 increased the water permeability of aquaporin (AQP0) 3.4 +/- 0.4-fold.

178 The water permeability of biological membranes has been a lo

179 The water permeability of both proteins was tested using the

180 the observation of the tremendously enhanced water permeability of carbon nanotubes, those iconic obj

181 ation was dominant, which in turn caused the water permeability of chlorinated membrane to decrease.

182 Osmotic water permeability of cortical collecting-duct basolater

183 In contrast, the corresponding water permeability of dendrimer-modified membranes decre

184 +) caused a two- to fourfold increase in the water permeability of endogenous AQP0.

185 of these vesicles was used to determine the water permeability of endogenously expressed AQP0.

186 Water permeability of G1-NH2 modified membrane decreased

187 ion was used to test mechanical strength and water permeability of giant-fluid bilayer vesicles compo

188 Glycerol and water permeability of GlpF both occur with low Arrhenius

189 traethylammonium (TEA) chloride, reduces the water permeability of human AQP1 channels expressed in X

190 ing on L(o), and its relationship to osmotic water permeability of isolated root cortex protoplasts,

191 The water permeability of liposomal membrane is found to be

192 .5 caused a two- to fourfold increase in the water permeability of mammalian AQP0.

193 A method is proposed to measure the water permeability of membrane channels by means of mole

194 oocytes showed that it increased the osmotic water permeability of oocytes 5-fold; this water transpo

195 ants, was found between Kros and the osmotic water permeability of protoplasts from the veins but not

196 The water permeability of SjAQP was inhibited by 1 mM HgCl2,

197 The water permeability of the fouled membranes was lowered b

198 ment of Sjogren's syndrome by increasing the water permeability of the gland to restore saliva flow.

199 demonstrated that vasopressin increases the water permeability of the inner medullary collecting duc

200 nine vasopressin (AVP) regulates the osmotic water permeability of the kidney collecting duct by indu

201 cell membrane and would account for the high water permeability of the lung.

202 nd thus contribute to the fine-tuning of the water permeability of the plasma membrane.

203 In this study, the water permeability of thermally reduced GO membrane was

204 However, the low water permeability of these membranes significantly limi

205 cm/s indicated a substantial single channel water permeability of UT-B of 7.5 x 10(-14) cm(3)/s, sim

206 We report that the water permeability of wild-type Escherichia coli during

207 a low but measurable enhancement in osmotic water permeability of Xenopus oocytes and hence represen

208 ted the effects of external pH and Ca(2+) on water permeability of Xenopus oocytes injected with aqua

209 of the mutant AQP1 channels showed that the water permeability of Y186F was equivalent to that of wi

210 However, in contrast to wild-type AQP1, the water permeability of Y186F was not reduced with 100 mic

211 Membrane water permeabilities (P(f)(mem)) of calcein-stained surf

212 Tissue water permeabilities (P(f)(tiss)) across intact cornea a

213 hile the M278T mutation increased normalized water permeability (P < 0.001).

214 d with WT), and no change in the diffusional water permeability (P(d)).

215 panied by a 2.8-fold increase in the osmotic water permeability (P(f) 280 +/- 40% compared with WT),

216 t scattering assays to have elevated osmotic water permeability (P(f) = 57 microm x s(-1) versus 12 m

217 , resulting in a approximately 5-fold higher water permeability (P(f)) across the blood-brain barrier

218 is an aquaglyceroporin with a modest osmotic water permeability (P(f)) and the ability to transport u

219 llecting duct, vasopressin increases osmotic water permeability (P(f)) by triggering trafficking of a

220 Osmotic water permeability (P(f)) in response to a 200 mM NaCl g

221 ses predict different values for the osmotic water permeability (P(f)) of airway epithelia.

222 Airspace-capillary osmotic water permeability (P(f)) was measured in isolated perfu

223 onate; net water movement (J(v)) and osmotic water permeability (P(f)) were then calculated.

224 accessible surface, channel gating dynamics, water permeability (p(f)), and a dihedral angle are defi

225 AQPxlo expression increased osmotic water permeability (P(f)), as well as the uptake of glyc

226 The AQP4 single channel water permeability (p(f)), estimated from Pf and protein

227 evels and decreased mesophyll and BS osmotic water permeability (P(f)), mesophyll conductance of CO2,

228 Only AQPs exhibited significant osmotic water permeability (P(f)).

229 We found high transepithelial water permeability (P(f), 54 +/- 5 microm/s) in airway e

230 d (ABA) or by high humidity, and the osmotic water permeability (P(os)) of protoplasts.

231 Diffusive water permeability (Pd) was 0.0024 +/- 0.0002 cm/s, and

232 ergy (Ea), mercurial inhibition, diffusional water permeability (Pd), and AQP expression.

233 ypertonic solution decreased the diffusional water permeability (Pdw) of the corneal epithelium.

234 f stopped-flow experiments yielded a unitary water permeability pf of (6.9 +/- 0.6) x 10(-13) cm(3)s(

235 rodissected plasma membranes, single channel water permeabilities (pf, referenced to the AQP1 pf of 6

236 eoliposomes, AqpZ displays very high osmotic water permeability (pf > or = 10 x 10(-14) cm3 s-1 subun

237 dose-dependent increases in osmotic membrane water permeability (Pf) (e.g. increased by 60% with 10(-

238 a, contributes to the modulation of membrane water permeability (Pf) and pH aquaporin regulation.

239 ression is associated with increased osmotic water permeability (Pf) between the airspace and capilla

240 Transepithelial osmotic water permeability (Pf) in isolated microperfused S2 seg

241 Osmotic water permeability (Pf) in plasma membrane vesicles from

242 se of this study was to test whether osmotic water permeability (Pf) in type I alveolar epithelial ce

243 Osmotic water permeability (Pf) in Xenopus oocytes injected with

244 ABA induced a 2-fold increase in osmotic water permeability (Pf) of guard cell protoplasts and an

245 Osmotic water permeability (Pf) of SM was high, with a value of

246 Quantitative measurement of single channel water permeability (pf) using epitope-tagged rat UTs gav

247 glucose transport was decreased, and osmotic water permeability (Pf) was increased.

248 Osmotic water permeability (Pf) was measured in double-perfused

249 Airspace-capillary osmotic water permeability (Pf) was measured in isolated perfuse

250 In this study, transepithelial osmotic water permeability (Pf) was measured in isolated perfuse

251 n Xenopus oocytes for measurement of osmotic water permeability (Pf), [3H]glycerol uptake, and protei

252 tes to determine the coefficients of osmotic water permeability (Pf).

253 gave a mercury-sensitive increase in osmotic water permeability (Pf).

254 nd type II alveolar epithelial cells to lung water permeability, Pf was measured by stopped-flow ligh

255 ther increase in the PLR reduced the osmotic water permeability probably due to the occurrence of def

256 O) of 1000-2000 Da but also have a high pure water permeability (PWP) of 82.5-117.6 L m(-2) h(-1) bar

257 Here, we address the hypothesis that this water permeability response is mediated in part through

258 syntrophin, which manifest reduced astrocyte water permeability secondary to defective AQP4 plasma me

259 In addition to high water permeability, SM vesicles also show high mercury-s

260 carefully design a PRO membrane with a large water permeability, small B value, and reasonably small

261 hAQP1 water channels can transit from a high-water-permeability state to a closed state.

262 nt calmodulin inhibitors each increased AQP0 water permeability, suggesting that Ca(2+) may act throu

263 AQP1 protein and had 7-fold greater osmotic water permeability than did erythrocytes from null mice.

264 understanding why mammalian AQP0 has a lower water permeability than other aquaporins and the evoluti

265 Previously, the only known blockers of water permeability through aquaporin-1 (AQP1) water chan

266 onstitution of AfAQP significantly increased water permeability through planar lipid bilayers, P(M,H(

267 ollecting duct cells, resulting in increased water permeability through regulation of the aquaporin-2

268 Water permeability through single-file channels is affec

269 our variant AQP4 channels reduced normalized water permeability to between 26 and 48% of the referenc

270 AQP1-deficient cells, which increased their water permeability to that of wild-type cells, corrected

271 t constitutive surface expression levels and water permeability to wild-type AQP4.

272 oocytes exhibit significantly higher osmotic water permeability under basal conditions.

273 glycerol permeability more than 100-fold and water permeability up to 10-fold compared with control l

274 Occasionally an open state, with diffusive water permeability very close to that of WT AqpZ, was ob

275 Osmotic water permeability was >4-fold reduced in -/- than in +/

276 Erythrocyte water permeability was 7-fold reduced by AQP1 deletion b

277 system, and the effect of truncation on AQP0 water permeability was assessed in an oocyte osmotic swe

278 Although increased water permeability was confirmed in the Xenopus oocyte e

279 The reduction of water permeability was dose-dependent for tested concent

280 Osmotic water permeability was immeasurably small.

281 Osmotic water permeability was measured by stopped-flow light sc

282 Water permeability was measured for eight GOALs characte

283 Osmotic water permeability was measured in calcein-stained epith

284 passage through an aqueous channel, osmotic water permeability was measured in Xenopus oocytes expre

285 not increased above background, and osmotic water permeability was only minimally elevated.

286 embrane vesicles from knockout mice, osmotic water permeability was reduced 8-fold compared with vesi

287 oocytes for 15 min with 100 microM TEA, AQP1 water permeability was reduced by 20 to 40%, a degree of

288 ion, membrane blebbing dynamics, and osmotic water permeability were assayed.

289 Changes in water permeability were associated with the overexpressi

290 ons after residues 234, 238, and 243 on AQP0 water permeability were examined.

291 The measurements of water permeability were found to scale exponentially wit

292 at specifically reduces AVP-elicited osmotic water permeability when luminal calcium rises.

293 rip, and Eglp2 show significant and specific water permeability, whereas Eglp2 and Eglp4 show very hi

294 ults in proteinuria and increased glomerular water permeability, which are both rescued by over-expre

295 This high water permeability, which is about 50% of aquaporin Z's

296 elled under hyposmotic conditions indicating water permeability, which was abolished after preincubat

297 s, could explain the observed differences in water permeability with changes in bilayer thickness.

298 the per-channel increment in proteoliposome water permeability with the aid of stopped-flow experime

299 ave physiological consequences, because AQP4 water permeability would be reduced by its sequestration

300 ess aquaporins and thus display low inherent water permeability, yet SD rapidly induces focal swellin