ライフサイエンスコーパス: whole-cell

1 importance of targeting PD-L1 throughout the whole cell.

2 l view of ultrastructural variability across whole cells.

3 TonB-dependent vitamin B(12) transporter, in whole cells.

4 on binding, and time-resolved FRET assays in whole cells.

5 of limited lifetime such as cell lysates or whole cells.

6 plex indistinguishably from that observed in whole cells.

7 g the systems-level understanding of iron in whole cells.

8 parent in both model membranes as well as in whole cells.

9 to look at cellular bioenergetic function in whole cells.

10 urfaces pre-modified with biomolecules or by whole cells.

11 de (FMN) riboswitch, from a compound lacking whole-cell activity against wild-type GN pathogens into

12 Maximization of whole-cell affinities may enable these organisms to comp

13 However, previous whole-cell analyses lacked the resolution to unravel the

14 A single whole-cell analysis using a serial section array (SSA)-S

15 Here, using whole cell and membrane proteomics, we show that the del

16 operate this instrument to acquire images of whole cells and analyze the raw image data to reconstruc

17 Both whole cells and cell lysates of Oxf strains HC1 and OxWR

18 oth elevated amounts of MeHg associated with whole cells and higher MeHg association rate constants i

19 However, conventional whole-cell and biochemical antibiotic screens have faile

20 r selection, aiding the rapid development of whole-cell and cell-free biosensors for various applicat

21 Both whole-cell and cell-free formats were investigated to as

22 by computer-aided tools, implemented in both whole-cell and cell-free systems.

23 Whole-cell and cell-free transcription-translation biose

24 Quantitative MS on whole-cell and centriolar satellite proteomes of acentri

25 Once integrated into circuits, they exhibit whole-cell and microdomain Ca(2+) transients, which are

26 In both whole-cell and microsome-based assays, 4-methoxyindol-3-

27 Here, we use whole-cell and perforated-patch recordings to test for G

28 20beta-HSDH activity was confirmed through whole-cell and pure enzymatic assays, and it is specific

29 voltage sensors contain the E160R mutation, whole-cell and single-channel currents are still observe

30 the aquatic pipid frog Xenopus laevis Using whole-cell and single-channel electrophysiology of Xenop

31 nd consequently greater Ca(2+) influx at the whole-cell and single-synapse levels, which also showed

32 y without altering protein expression at the whole-cell and surface levels.SIGNIFICANCE STATEMENT We

33 ctivity with purified protein, cell lysates, whole cells, and in human gut microbiome samples.

34 must limit the total fluorescence output of whole cells, and that improving folding efficiency could

35 est is an immunofluorescence assay utilizing whole cell antigens, which are both dangerous and labori

36 f the current analogues, indicating that the whole-cell antimycobacterial activity of the present S-s

37 t cardiomyocytes by simultaneously recording whole-cell apamin-sensitive currents and Ca(2+) transien

38 eted redox-active metabolites/compounds from whole cells are likely contributing to the signal as wel

39 the chemical structure of polyphosphates in whole cells as well as insoluble fractions of the bacter

40 Reactions using E. coli whole cells as well as purified enzymes produced excelle

41 be the development of a sensitive and robust whole cell assay to enable the large-scale profiling of

42 ectively, these results demonstrate that the whole-cell assay developed here can be used to uncover n

43 We describe reporter gene whole cell-based biosensor systems based on luciferase g

44 unds was also confirmed in Proteus mirabilis whole-cell-based inhibition assays.

45 A direct peptide array-whole cell binding assay, where the peptides are conjuga

46 protected, and enhanced the reactivity of a whole cell biocatalyst.

47 in libraries to identify the best-performing whole-cell biocatalysts is a low-throughput endeavor.

48 the specific turnover numbers of individual whole-cell biocatalysts.

49 sion from 10 mM cyclohexylamine by employing whole-cell biocatalysts.

50 A whole-cell biophysical model using Hodgkin-Huxley equati

51 Our whole-cell biosensor is simple and low-cost and therefor

52 Whole-cell biosensors present many advantages, including

53 ex vivo responses by direct ELISpot to both whole-cell C. burnetii and individual peptides in chroni

54 Store-operated whole-cell cation currents were blocked by Pico145, a hi

55 We used a simple whole-cell coarse-grained model of cell physiology that

56 Patch-clamp whole-cell configuration analysis of pollen grain protop

57 MOC terminals in voltage-clamped IHCs in the whole-cell configuration.

58 With specifically designed single-whole-cell confocal micro-Raman spectroscopy, quantitati

59 Whole cell CRAC current is Ca(2+) -selective.

60 tude, rectification or reversal potential of whole-cell CRAC current.

61 n pathogen Mycoplasma pneumoniae We combined whole-cell cross-linking mass spectrometry, cellular cry

62 tromatoxin was used to block Kv2 currents in whole-cell current clamp electrophysiological recordings

63 fit voltage-sensitive ion channel models to whole-cell current measurements: method 1, fitting model

64 ly, we investigated whether insulin augments whole-cell current responses to mechanical stimuli in sm

65 Whole-cell current-clamp recordings in cultured Schwann

66 To investigate, we obtained whole-cell current-clamp recordings of pyramidal neurons

67 -methyl-d-aspartate (NMDA) receptors, affect whole cell currents only after several hours incubation

68 conductance at positive potentials, whereas whole-cell currents at negative potentials, although mar

69 d using the patch clamp technique to measure whole-cell currents in muscle-type (TE671/CN21) and non-

70 Analyzing whole-cell data from mice running on virtual tracks, we

71 t reduced chloride currents, and accelerated whole-cell desensitization observed in whole-cell record

72 parameters of the ion channel (e.g., faster whole-cell desensitization) reduced unitary conductance

73 We conclude that whole cell dynamics requires balance between the higher-

74 In addition, ex vivo whole-cell electrophysiological recordings from female l

75 Whole-cell electrophysiological recordings of D1-MSNs we

76 o explore the impact of molecular changes on whole cell electrophysiology and calcium cycling.

77 ors that were tested in glutamate uptake and whole-cell electrophysiology assays.

78 Whole-cell electrophysiology demonstrated that the intac

79 In whole-cell electrophysiology experiments, we identified

80 Previously, we used whole-cell electrophysiology in Drosophila to show that

81 In parallel, we conducted whole-cell electrophysiology recordings in the NAc of st

82 tioned place preference behavior and ex vivo whole-cell electrophysiology showed that cocaine-primed

83 we combined two-photon calcium imaging with whole-cell electrophysiology to determine how action pot

84 Using optogenetics with extracellular and whole-cell electrophysiology, we assessed the effect of

85 Using confocal imaging and whole-cell electrophysiology, we demonstrate that overex

86 Using whole-cell electrophysiology, we show here that heterolo

87 Here we propose a label-free, optical whole-cell Escherichia coli biosensor for the detection

88 ce was evaluated for biotransformation using whole cells exemplified for the dehydration of n-octanal

89 ations, including comprehensive profiling of whole-cell, exosomal, and human plasma RNAs; quantitativ

90 es contrasts with previous observations from whole cell experiments and suggests that specific uptake

91 ncreased levels of ubiquitinated proteins in whole-cell extract and at proteasomes, suggesting that U

92 In contrast, (31)P NMR of whole-cell extract from capsule-knockout strains (Deltac

93 ne poly(A)(+) RNA interactome capture with a whole-cell extract normalization procedure.

94 However, when utilizing HeLa whole cell extracts, the difference in incision of THF-i

95 ombination of E3-selective ubiquitination in whole-cell extracts and high-resolution MS.

96 ps by systematically comparing nuclear-FACS, whole cell-FACS, and RiboTag affinity purification from

97 e diol derived from phenethyl acetate by the whole-cell fermentation with E. coli JM109 (pDTG601A), a

98 Here, a whole-cell fluorescence biosensor for 2'-FL was develope

99 erived pigments, especially carotenoids, and whole-cell focused ion-beam scanning-electron microscopy

100 Notably, immunization with fimbrial-whole-cell Footvax vaccine induced anti-Dn-ACP and anti-

101 e of de novo metabolic pathways and designer whole-cells for small molecule synthesis, the inherent s

102 Maternal vaccination with whole-cell GBS induces production of GBS-specific IgG in

103 the impact of maternal immunization using a whole-cell GBS vaccine on the duration of intestinal col

104 l external Ca(2+), we observed robust CDI of whole-cell GluN2A currents (0.42 +/- 0.05) but no CDI in

105 Thus, we complement the surfaceome data with whole cell glycoproteomics enabled by a recently develop

106 th LGR5 interacting with these E3 ligases in whole cells has not been reported, and only LGR4 is esse

107 In this work, we developed a whole-cell high-throughput hydrogenase assay based on th

108 at a specific subcellular location even with whole-cell illumination, allowing us to confidently prob

109 sis which combines the principle of PDD with whole cell immunocapture technology to detect bladder ca

110 Whole cell impedance sensing, also known as electric cel

111 antum dot for detection Campylobacter jejuni whole cell in food samples was designed.

112 measured the fluorescence emitted at 77 K by whole cells in a quenched or unquenched state, using gre

113 proteins as recombinant antigens (RCA) and a whole-cell inactivated antigen vaccine (WCA), in conferr

114 rulent (PI-WCV), but not avirulent (PII-WCV) whole-cell inactivated bacterium.

115 nisotropic (31)P chemical shifts of hydrated whole cells indicate the coexistence of linear and cycli

116 ae-seroreactive samples cross-reacted with a whole-cell indirect fluorescent antibody (IFA) test and

117 However, cryoET of whole cells is technically difficult.

118 In this study, whole-cell L- (nimodipine-sensitive), N- and P/Q- (omega

119 nsitive response of integrin mobility at the whole cell level and in adhesion sites under different m

120 res both spatially resolved cleft as well as whole cell level descriptions.

121 that the valency model can be applied at the whole cell level to study differences in individual cell

122 At the whole-cell level, knockout of retromer Vps35 subunit red

123 ion specifically in synapses, and not at the whole-cell level.

124 ractant concentration while decreasing their whole-cell levels.

125 titers of antibodies against LcrV, Y. pestis whole-cell lysate (YPL), and F1 antigen and more balance

126 e knockdown also increased phospho-VEGFR2 in whole cell lysates and membrane fractions compared with

127 ully applied to detect pathogenic Leptospira whole cell lysates samples with the satisfactory results

128 d sequence coverage when applied to S. pombe whole cell lysates.

129 00HP expressed equivalent amounts of HgbA in whole-cell lysates and outer membranes.

130 y, we developed, to date, the most sensitive whole-cell mercury biosensor using NanoLuc as reporter,

131 Functional metabolism studies, profiling of whole-cell metabolites, and analysis of cell surface pro

132 imal amount of bioelectricity generated from whole-cell microalgae.

133 Application of step indentations under the whole-cell mode of the patch-clamp technique, and positi

134 sed the first (and currently only published) whole-cell model for the bacterium Mycoplasma genitalium

135 erable parameter regimes We have developed a whole-cell model of iPSC-CMs, composed of single exponen

136 Efforts to build predictive, whole-cell models of E. coli inevitably face this knowle

137 ows for rapid and accurate reconstruction of whole cell morphology of large neuronal populations in d

138 rive both flow of cytoplasmic organelles and whole-cell movement-analogous to the autonomous motility

139 rrelations demonstrate the limits of mapping whole-cell mRNA numbers to the underlying stochastic gen

140 veal new insights into the complexity of the whole cell muscle mRNA of Nelore cattle.

141 of SLC25A51 decreases mitochondrial-but not whole-cell-NAD(+) content, impairs mitochondrial respira

142 , as found in human patients, still supports whole-cell NALCN currents but lacks dendritic localizati

143 When foci are reconstructed in the whole cell nucleus, we obtain information on damage char

144 Membrane transport experiments conducted in whole cells of A. baumannii and Escherichia coli and als

145 Following injection of formalin-fixed whole cells of C. perfringens HN13 (a laboratory strain)

146 raction for nutraceuticals) for fishmeal and whole cells of docosahexaenoic acid (DHA)-rich Schizochy

147 (induction) and relaxation (in the dark) for whole cells of photosynthetic bacterium Rhodobacter spha

148 Here, we compared the lipid composition of whole cells of the pigmented E. gracilis strain Z and tw

149 tive means of characterizing its activity in whole cell or microbial community samples.

150 sis of purified samples and 2) tomography of whole cells or cell sections.

151 ally manipulate physiological processes over whole cells or in small subcellular volumes.

152 ump capacity was estimated from K(+)-induced whole cell outward current (pump capacity).

153 Proteinase K accessibility assays and whole-cell partitioning indicated that BB0345 was intrac

154 Whole cell patch clamp and dynamic action potential clam

155 Whole cell patch clamp electrophysiological recordings o

156 ) in the dorsal root ganglion (DRG), and the whole cell patch clamp was used to examine the amplitude

157 ibitions by purinergic receptor antagonists, whole cell patch-clamp recordings of ATP-induced current

158 icator dye and ionic current was recorded by whole cell patch-clamp.

159 ip and antagonistic mechanism on TRPM2 using whole-cell patch clamp and Calcium imaging in human embr

160 were heterologously expressed, recorded with whole-cell patch clamp and conolidine/cannabidiol was ap

161 Using whole-cell patch clamp electrophysiology we demonstrate

162 xpressed in tsA201 cells were measured using whole-cell patch clamp electrophysiology.

163 Furthermore, whole-cell patch clamp experiments and assessment of mem

164 hors purified KCNQ1 antibodies and performed whole-cell patch clamp experiments as well as single-cha

165 We performed whole-cell patch clamp recordings using cultured DRG neu

166 In whole-cell patch clamp recordings, we observed larger ma

167 physical properties were further assessed by whole-cell patch clamp technique.

168 Pharmacological, electrophysiological (whole-cell patch clamp), and behavioral approaches were

169 Using whole-cell patch clamp, we found that the activation of

170 n of mefenamic acid's effect on I (Ks) Using whole-cell patch clamp, we show that mefenamic acid enha

171 ust activation by PMA that was absent during whole-cell patch clamp.

172 choline-induced currents were measured using whole-cell patch clamping and a fast perfusion system to

173 Whole-cell patch clamping demonstrates that plumbagin an

174 se a combination of protein biochemistry and whole-cell patch clamping to determine effects of diet m

175 ctric mapping, isolation of atrial myocytes, whole-cell patch clamping, in vitro tachypacing of atria

176 Here, we show that supplementing the whole-cell patch pipette with PI(4,5)P(2) reduced inhibi

177 milar manner to the parent dyes, as shown by whole-cell patch recording.

178 Whole-cell patch recordings from brainstem slices of mic

179 in the PVH following vagal stimulation, and whole-cell patch recordings of GLP-1 receptor-expressing

180 A multifaceted approach, including in vitro whole-cell patch recordings, in vivo single-unit recordi

181 By combining whole-cell patch-clamp and 2-photon laser scanning micro

182 x months after SE or sham treatment, we used whole-cell patch-clamp and fluorescence microscopy to re

183 Whole-cell patch-clamp electrophysiology subsequently re

184 ng male D1tdTomato transgenic reporter mice, whole-cell patch-clamp electrophysiology, and input-spec

185 Using whole-cell patch-clamp electrophysiology, inhibitory GAB

186 her mutations in HEK293T cells and performed whole-cell patch-clamp electrophysiology.

187 in the first postnatal weeks using in vitro whole-cell patch-clamp electrophysiology.

188 Whole-cell patch-clamp experiments in acute mouse brains

189 In whole-cell patch-clamp experiments, we examined the effe

190 t reduces the IC(50) of TETS by ~700-fold in whole-cell patch-clamp experiments.

191 t ganglion (DRG) neurons was examined by the whole-cell patch-clamp method.

192 acute passive infusion of alphaSyn PFFs from whole-cell patch-clamp pipette decreased mEPSC frequency

193 Here, using in vitro whole-cell patch-clamp recording and intracellular filli

194 Using the whole-cell patch-clamp recording, we found that THIK-1 c

195 By performing whole-cell patch-clamp recording, we showed that TMEM16B

196 We used whole-cell patch-clamp recordings and an optogenetic app

197 Using whole-cell patch-clamp recordings combined with optogene

198 Whole-cell patch-clamp recordings demonstrate that loss

199 Acute whole-cell patch-clamp recordings from GrCs in brain sli

200 Whole-cell patch-clamp recordings from neurons in PFC sl

201 xpressed in tsA201 cells were measured using whole-cell patch-clamp recordings in the presence and ab

202 We performed whole-cell patch-clamp recordings of medium spiny neuron

203 Whole-cell patch-clamp recordings of neurons from male m

204 Whole-cell patch-clamp recordings of striatal spiny proj

205 Whole-cell patch-clamp recordings reveal that detection

206 First, we used whole-cell patch-clamp recordings to measure the physiol

207 Whole-cell patch-clamp recordings were obtained from Pur

208 sciplinary approach included Ca(2+) imaging, whole-cell patch-clamp recordings, skin-nerve and gut-ne

209 By combining single-cell RNA-seq with whole-cell patch-clamp recordings, we identified Ptgds a

210 obustly blocks anesthetic TREK-1 currents in whole-cell patch-clamp recordings.

211 say, intracellular calcium measurements, and whole-cell patch-clamp recordings.

212 ce energy transfer, L-type Ca(2+) current by whole-cell patch-clamp, and cardiomyocyte shortening and

213 injection of pseudorabies virus PRV-152) and whole-cell, patch clamp recordings were obtained in brai

214 We used the whole-cell, patch clamp technique to characterize the ef

215 ded from rabbit and human atrial myocytes by whole-cell-patch clamp.

216 king memory processing, in vitro brain slice whole-cell patching recordings and in vivo stereotaxic h

217 y monitoring junctional conductance via dual whole-cell/perforated patch clamp.

218 ancy on infant antibody responses induced by whole cell pertussis (wP) vaccination is not well-define

219 ancy on infant antibody responses induced by whole-cell pertussis (wP) vaccination is not well-define

220 , acellular pertussis (aP) vaccines replaced whole-cell pertussis (wP) vaccine in the primary immuniz

221 NCT02408926.Infant whole-cell pertussis (wP) vaccine responses are blunted

222 ose born 1950 or later who may have received whole-cell pertussis vaccine (53%; -11% to 80%); p-heter

223 ose born 1950 or later who may have received whole-cell pertussis vaccine (53%; -11-80%) (P-heterogen

224 middle-income countries, nor from ones using whole-cell pertussis vaccines for primary immunization.

225 We establish that whole-cell physiological recordings reveal tuning of ind

226 Here, we show that fission yeast whole-cell poly(A)(+) RNA-protein crosslinking data prov

227 Whole cell protein lysates were collected and analysed u

228 Bartonella henselae San Antonio type 2 (SA2) whole-cell proteins, sera derived from four dog groups w

229 ractome of Prxs at the level of a eukaryotic whole cell proteome.

230 itylated proteins, which in combination with whole-cell proteomic and transcriptomic data allowed pre

231 Characterisation of abundant proteins in whole cells provided evidence of the time dependent tran

232 By combining in vivo whole-cell recording and pharmacological silencing of co

233 llular application of apamin via the somatic whole-cell recording pipette reduced the medium afterhyp

234 By combining imaging, whole-cell recording, pharmacology, and anatomical techn

235 Whole cell recordings demonstrated that elevated CO(2) r

236 To address this, we established in vivo whole-cell recordings and obtained over 100 spontaneousl

237 Whole-cell recordings from GoCs revealed a role for the

238 Whole-cell recordings from locus coeruleus neurons were

239 wing action potentials (APs), as measured in whole-cell recordings from male mice running in virtual

240 Whole-cell recordings from SOM-INs revealed that both ex

241 In contrast, using dual whole-cell recordings from the apical dendrite and soma

242 naptic inputs and intrinsic properties using whole-cell recordings from V1 neurons of awake, fixating

243 Whole-cell recordings in basolateral amygdala (BLA) slic

244 Whole-cell recordings in BLA pyramidal neurons revealed

245 putations underlying predictive coding using whole-cell recordings in mouse visual cortex.

246 We employ in vivo whole-cell recordings in the mouse primary auditory cort

247 Whole-cell recordings of adjacent newly generated neuron

248 Whole-cell recordings of arcuate kisspeptin neurons in b

249 Patch-clamp whole-cell recordings of KCNN3 channel-expressing CHO ce

250 Using whole-cell recordings of NTS neurons, from horizontal br

251 erformed calcium imaging, cell-attached, and whole-cell recordings of PC and PV cells within the CA1

252 Whole-cell recordings of PCs from acute cerebellar slice

253 siological parameters were derived from dual whole-cell recordings of pyramidal cells in organotypic

254 Here we used whole-cell recordings of T5 neurons and found a similar

255 d Ca(2+) imaging on mice from both sexes and whole-cell recordings on female mouse TIDA neurons in vi

256 In vivo whole-cell recordings reveal that mechanisms contributin

257 Whole-cell recordings showed that small-diameter Vglut3(

258 LNv whole-cell recordings then show that changes in Shaw and

259 Here, we combined optogenetic and whole-cell recordings to draw a functional portrait of e

260 ions were discovered [15], we used quadruple whole-cell recordings to screen connectivity within the

261 Here we combine multiple simultaneous whole-cell recordings with extracellular stimulation and

262 Using whole-cell recordings with morphological recovery, we id

263 itro; further analysis with genetic markers, whole-cell recordings, and single-cell transcriptomics v

264 In whole-cell recordings, application of a high-affinity Y(

265 (2-p) Calcium (Ca) imaging and 2-p targeted whole-cell recordings, we cell-type specifically investi

266 Using whole-cell recordings, we found that the alpha3beta4* nA

267 th extracellular multiunit and intracellular whole-cell recordings, we found that the magnitude and r

268 Here, using in vivo whole-cell recordings, we show that a visual cue can evo

269 rated whole-cell desensitization observed in whole-cell recordings.

270 Whole cell responses involve multiple subcellular proces

271 l SCPs involved and imbalances can terminate whole cell responses such as neurite outgrowth.

272 alance between SCPs controls the dynamics of whole cell responses we studied neurite outgrowth in rat

273 sistant to perchlorate inhibition, mirroring whole cell results.

274 is of atrazine in chemostat experiments with whole cells revealed a drastic decrease in isotope fract

275 High-throughput whole-cell screening of an extensive compound library ha

276 The whole-cell sensor will be versatile in developing a 2'-F

277 sessed T-tubules sodium current by recording whole-cell sodium currents in control (N=5) and detubula

278 Serum specimens were analyzed with both a whole-cell sonicate (WCS) and a C6 EIA, with a supplemen

279 al no change in the maximum conductance, and whole-cell studies, which reveal a dramatically altered

280 ey are less expensive than purified enzymes, whole cells suffer from inherent reaction rate limitatio

281 dal activity and did not block glycolysis in whole cells, suggesting a role for the lipid moiety in t

282 functions like a competitive ELISA but uses whole-cells surface displaying an anti-3-PBA VHH as the

283 d cancerous prostate cell lines based on the whole-cell, time-resolved mechanical response to a hydro

284 (ASTAR-seq), for simultaneous measurement of whole-cell transcriptome and chromatin accessibility wit

285 In combination with whole-cell transformations, the myoglobin-based biocatal

286 In combination with whole-cell transformations, these biocatalysts enabled t

287 ytotoxicity and hemolysis assays, as well as whole-cell uptake and development of resistance studies.

288 The formalin-inactivated whole-cell vaccine (WCV) confers long-term protection bu

289 Vaccination with crude whole-cell vaccine mixed with multiple recombinant fimbr

290 hanism of responsiveness to vaccination with whole cell vaccines against TB or in vitro stimulation w

291 t of their safe and effective application as whole-cell vaccines against leishmaniasis.

292 al administration of adjuvanted, inactivated whole-cell vaccines for O157 can induce O157-specific ce

293 ed to extracellular superoxide production by whole cells via quenching by the flavoenzyme inhibitor d

294 Using whole-cell voltage clamp of Nav1.6, we show that CaMKII

295 Using whole-cell voltage clamp, we discovered that enhancing S

296 ssed in HEK293 cells were recorded using the whole-cell voltage-clamp method.

297 Synaptic currents were recorded in whole-cell voltage-clamped OHCs while electrically stimu

298 ple Z-section images, allowing us to acquire whole cell volumes in which to scan for centrosomes.

299 IR maps of the whole cell were analyzed by exploiting the RE-AFM-IR ove

300 Selection/counter-selection on transfected whole cells yielded hCD20-specific antibodies in four ro