ライフサイエンスコーパス: wobble

1 CUG) anticodon stem that restrict first base wobble.

2 interacts with a newly resolved G.U reverse wobble.

3 ighboring base pairs on only one side of the wobble.

4 ls moved downward at low forces with minimum wobble.

5 al hydration sphere with the G25.U20 reverse wobble.

6 rse wobble with an isosteric A25.C20 reverse wobble.

7 lso a UAG nonsense suppressor via first base wobble.

8 the means by which s(2)C(32) restricts I(34) wobbling.

9 -derived metabolites required to confer tRNA wobbling.

10 unexpected result, however, is that a highly wobbled A.T base pair, which is ascribed here to a rare

11 )) were essential for Watson-Crick (AAA) and wobble (AAG) cognate codon recognition by tRNA(UUU)(Lys)

12 ifications also play a role in accommodating wobble, allowing a limited pool of tRNAs to recognize de

13 pe II tandem G.U pairs have a combination of wobble and bifurcated hydrogen bonds where the uracil 2-

14 ease mismatch discriminations (including T/G wobble and T/C mismatched base pairs) while maintaining

15 guide-target interactions we introduced G:U wobbles and mismatches at various positions of the micro

16 ions, including combinations of multiple G:U wobbles and mismatches in the seed region, are admissibl

17 the rate-accuracy variation for 7 cognate, 7 wobble, and 56 near-cognate codon readings comprising ab

18 three mismatched base-pairs: an A+-C, a G-U wobble, and a sheared G-A base-pair and no looped out ba

19 and the MT wall should cause a Dam1 ring to wobble, and Fourier analysis of moving, ring-attached be

20 n polar angle and a ~0.3pai sr difference in wobble angle.

21 The cationic PDI produced a wobbling angle distribution that was broader than the ot

22 The anticodon wobble at position 34 and the nucleotide immediately 3'

23 Watson-Crick base pairing properties of the wobble base (and hence proper translation of the genetic

24 could be developed that would alter the tRNA wobble base base pairing properties.

25 We show that U:T wobble base interactions are critical to prevent extensi

26 is unable to rectify a break at the modified wobble base of tRNA(Glu(UUC)).

27 Trm9 methylates the uridine wobble base of tRNAARG(UCU) and tRNAGLU(UUC).

28 air (on the guanine containing strand) and a wobble base pair (on the strand containing the difluorot

29 containing a bulged nucleotide adjacent to a wobble base pair also was primarily affected by non-near

30 other hand, recognizes the TG mismatch as a wobble base pair and penetrates the DNA with three aroma

31 he templating base, Poliota accommodates the wobble base pair better than the Watson-Crick base pair.

32 he ribozyme and the data here show that this wobble base pair destabilizes neighboring base pairs on

33 econciled by possible tautomerization of the wobble base pair in mRNA-tRNA.

34 laRS) has depended predominantly on a single wobble base pair in the acceptor stem, G3*U70, mainly on

35 Importantly, the mcm(5)s(2)U(34).G(3) wobble base pair is in the Watson-Crick geometry, requir

36 ent upon the position and orientation of the wobble base pair relative the bulged nucleotide.

37 -thymine mispairs may be associated with its wobble base pair structure.

38 Mutation of the U50.G64 wobble base pair to C50:G64 or U50:A64 base pairs increa

39 nt for elongation and that it is the U50.G64 wobble base pair, located at the same position in the TP

40 or both of the bulge nearest neighbors was a wobble base pair, the free energy increment for insertio

41 n interacts with N7 of the cleavage site G.U wobble base pair.

42 state using a steric filter fashioned from a wobble base pair.

43 e modified base pair in the structure adopts wobble base pairing (hydrogen bonds between [POB]dG(N1)

44 er, interactions between adjacent codons and wobble base pairing are key.

45 The former is most likely due to wobble base pairing between ClU and G, which may be more

46 The helical distortions and wobble base pairing induced by the covalent binding of P

47 G.U wobble base pairing is tolerated as a match for both RNA

48 2 genome excision leads to the disruption of wobble base pairing of SsrA due to site-specific recombi

49 translation speed including mRNA structure, wobble base pairing, tripeptide motifs, positively charg

50 However, the wobble base pairing, where T pairs with G instead of A,

51 tion of miRNA base pairing or by creation of wobble base pairing.

52 G.U wobble base pairs are the most common and highly conserv

53 side chain plays a pivotal role in excluding wobble base pairs between template pyrimidines and purin

54 The wobble base pairs contribute to the catalytic rate enhan

55 ostatic potential at the major groove of G.U wobble base pairs embedded in RNA helices, suitable for

56 reases the stability of duplexes closed with wobble base pairs in an idiosyncratic manner.

57 at the negativity at the major groove of G.U wobble base pairs is determined by the combined effect o

58 d melting temperature for duplexes closed by wobble base pairs with 3' single or double-nucleotide ov

59 polymerase active site and the asymmetry of wobble base pairs, provides a plausible explanation for

60 However, the wobble base pairs, where U in RNA (or T in DNA) pairs wi

61 n both sides by cis Watson-Crick G/C and G/U wobble base pairs.

62 While existing data link wobble base U34 modifications to translation of function

63 However, G:U wobble base-pairing in this region interferes with activ

64 on or by an artificial tRNA not dependent on wobble base-pairing.

65 Guanine-uracil (G.U) wobble base-pairs are a detrimental lesion in DNA.

66 Previous investigations have shown that such wobble base-pairs are more prone to base-opening than th

67 d, to a lesser extent, guanine residues from wobble base-pairs in hairpin stems.

68 ed 5ns molecular dynamics simulations on G.U wobble base-pairs in two different sequence contexts, TG

69 Incorporation of 6MI yields wobble base-pairs that open more readily than their guan

70 rmediate that involves DNA-base flipping and wobble base-pairs.

71 iting stimulates conversion of A to I at the wobble base.

72 one containing a central G-T mismatched or "wobble" base pair, and one in which the thymine in this

73 n an accurate but inefficient manner with a "wobble" base pairing between C and O(6)-MeG.

74 oaching that of its natural analogue, a G-T (wobble) base pair.

75 and molecular dynamics (MD) study of the G/U wobble basepairs in the ribosome based on high-resolutio

76 sults in the complete loss of these modified wobble bases and increased sensitivity at 37 degrees C t

77 imental systems have shown that systems may 'wobble' before a critical transition.

78 This sensitivity is explained if CTX wobbles between several bound conformations, producing t

79 nated at the N1 position to form stabilizing wobble CA+ pairs adjacent to a sheared GA or AA pair.

80 ree-energy model, including stabilization by wobble CA+ pairs, is derived for predicting stabilities

81 cated these tRNA modifications in modulating wobbling capacity and translation efficiency, their exac

82 the 5' single terminal overhangs adjacent to wobble closing base pairs are also presented.

83 For example, early hypoxia increases wobble cmo(5)U in tRNA(Thr(UGU)), which parallels transl

84 y of discrimination against near-cognate and wobble codon readings increased toward the maximal asymp

85 he s(2)C(32) modification is known to negate wobble codon recognition of the rare CGA codon by an unk

86 In contrast, other modifications expand wobble codon recognition, such as U*U base pairing, for

87 iscriminating between the correct cognate or wobble codons and the incorrect near-cognate codons (e.g

88 ance to tRNA's ability to decode cognate and wobble codons become apparent.

89 xhibited high affinities for its cognate and wobble codons GUA and GUG, and for GUU in the A-site of

90 L(Arg2)(ICG) constructs bound to cognate and wobble codons on the ribosome revealed the disruption of

91 ient and accurate recognition of cognate and wobble codons.

92 igase junction is predominantly in a neutral wobble configuration and is poorly ligated.

93 ] internal loop, the GU pairs form canonical wobble configurations with two hydrogen bonds, whereas i

94 atch is also poised for catalysis but in the wobble conformation seen in other studies, indicating th

95 absence of protein contacts, oxoA:G forms a wobble conformation, the formation of which is suppresse

96 he mismatched C(5).A(16) pair existed in the wobble conformation, with the C(5) imino nitrogen hydrog

97 TIPRL also makes unusual wobble contacts with the scaffold subunit, allowing TIPR

98 t sulfation patterns, which are termed here "wobble CS/DS oligosaccharide motifs," and induce signali

99 7 contain antisense elements that target the wobble cytidine at position 34 of human elongator tRNA(M

100 CAA-decoding tRNA(Gln)(UUG), an inefficient wobble-decoder of CAG.

101 anticodon stem and loop domain (ASL) negates wobble decoding of its synonymous A-ending codon, sugges

102 NMR relaxation dispersion, we show here that wobble dG*dT and rG*rU mispairs in DNA and RNA duplexes

103 R relaxation dispersion recently showed that wobble dG.dT and rG.rU mismatches in DNA and RNA duplexe

104 ition of thymidine, we have investigated the wobble discrimination by manipulating the steric and ele

105 hat the major groove edge of an isolated G.U wobble displays distinctly enhanced negativity compared

106 The extent of the local ring dynamic wobble does not increase, and may decrease slightly, whe

107 ion of the (31)P, while a slower rotation or wobble dominates the relaxation of the carbonyl carbon b

108 osition in microscopy imaging, and molecular wobbling during the image integration time can bias orie

109 her restricting codon recognition, expanding wobble, enabling translocation, or maintaining the messe

110 tures an unusually structured apical loop, a wobble-enriched, coaxially stacked apical and tetra-stem

111 The wobble G-T (wG-T), WC-like G-T*, and WC-like G*-T forms

112 not adjacent to an A.A pair forming a static wobble G.U pair.

113 er of non-canonical base pairs including the wobble G.U pairs were identified.

114 s indicate that the ClU-G base pair adopts a wobble geometry at neutral pH, similar to a T-G mispair.

115 s a Watson-Crick-like geometry rather than a wobble geometry, suggesting that the enol tautomeric for

116 urine-pyrimidine mispairs adopt the expected wobble geometry, the difference between the two polymera

117 tion and formed Hoogsteen pairing while in a wobble geometry, which was stabilized by Gln38-mediated

118 ing the number of strong (GC), weak (AU) and wobble (GU) base pairs to lie in a certain range, the RN

119 Removal of the wobble hydrogen bonds in U:dT recovers a strong response

120 The Modified Wobble Hypothesis proposed in 1991 that specific modific

121 One foundation of Crick's Wobble Hypothesis was that a near-constant geometry of c

122 d in the third base-pair, giving rise to the Wobble Hypothesis.

123 enrichment for codons decoded by I*A and U*G wobble in both Candida and Saccharomyces.

124 ( approximately 2 mus), while simultaneously wobbling in a cone of semiangle 30-55 degrees centered a

125 Orientational relaxation is analyzed with a wobbling-in-a-cone model describing restricted orientati

126 two periods of restricted angular diffusion (wobbling-in-a-cone) followed by complete orientational r

127 ing deprotonated G-C structure is a "reverse wobble" incorporating two N-H...N hydrogen bonds.

128 xpressing only ADAT3-V144M exhibit decreased wobble inosine in certain tRNAs.

129 role for ADAT2-dependent folding of ADAT3 in wobble inosine modification and indicate that proper for

130 d Mg(2)(+) positioned at the G25.U20 reverse wobble is catalytic and could serve as a Lewis acid, a B

131 ely because of averaging by fast motions and wobble; it is tentatively estimated to be 1 x 10(7) s(-1

132 ional shift of native Watson-Crick pair to a wobble-like pattern with the formation of two hydrogen b

133 Wobbling may originate from a network of contacts in the

134 es are attributed to inversion via a lateral wobble mechanism with DeltaH++ = 6 kcal x mol(-1) and De

135 fertility, suggesting a role of m(5) C tRNA wobble methylation in the adaptation to higher temperatu

136 These results support and extend our "wobble" model for CP binding to the actin filament, in w

137 Unlike classical "wobble" models, our analyses showed that three of four p

138 The human wobble modification has a less dramatic loop remodeling

139 Wobble modifications, t(6)A37, and magnesium each make u

140 d including an inertial motion, a restricted wobbling motion of approximately 3 ps, and complete rand

141 cence anisotropy decay and internal angular 'wobbling' motion measurements of 2-AP within these alter

142 LC activity, and slows nanosecond rotational/wobbling motions of both phospholipid headgroups, as ind

143 sicles, we found large-amplitude, rigid-body wobbling motions on the nanosecond time scale relative t

144 The time scales and amplitudes of these "wobbling" motions are characterized by effective correla

145 entifies three global motions: torsional and wobbling movements, en bloc, between the alpha- and beta

146 th longer inserts was made more efficient by wobble-mutagenizing both the inner repeat and the exogen

147 overcome by expression of a cDNA encoding a wobble mutant of FBL2.

148 lowing introduction of a DLX5 cDNA harboring wobbled mutations at the shRNA-targeting sites.

149 n 1991 that specific modifications of a tRNA wobble nucleoside shape the anticodon architecture in su

150 The wobble nucleotide positions of each codon were replaced,

151 he orientation spectra (3D orientation plus "wobble") of lipophilic probes transiently bound to lipid

152 tabilizing effects induced by the tandem G.U wobbles on the double-stranded structure of this stem, w

153 w these hexameric ATPases interact with and "wobble" on top of the heptameric 20S proteasome.

154 les: 3D spatial position, 3D orientation and wobbling or dithering angle.

155 The cis-5R,6S Tg lesion is in the wobble orientation such that Tg(6) O(2) is proximate to

156 dTTP opposite template N2,N2-Me2G revealed a wobble orientation.

157 a simple average of a back-and-forth display wobble over time.

158 ompanied by formation of an internal AH(+).C wobble pair [Siegfried, N.

159 mations for the P1.1 stem, the cleavage site wobble pair and the A-minor motif of the catalytic trefo

160 These conserved sequences include a u-G wobble pair at the 5' splice site and a guanosine in the

161 rcus A58 in the J4/5 region contacts the G.U wobble pair at the cleavage site in the P1 helix, and Az

162 ave hypothesized to discriminate against U/G wobble pair by tailoring the steric and electronic effec

163 The wobble pair existed as a mixture of protonated and nonpr

164 he presence of AFB(1) did not interfere with wobble pair formation at the mismatched site.

165 rms a stem-loop structure stabilized by a GU wobble pair formed by two of the five unpaired residues

166 ed pH-dependent formation of the A2450+C2063 wobble pair has made it a potential candidate for the pH

167 st a previously unrecognized role of the G.U wobble pair in self-splicing: breaking cooperativity in

168 trogen was similar to that of the C(5).A(16) wobble pair in the corresponding duplex not adducted wit

169 termini of these siRNAs with a terminal G-U wobble pair or a carefully selected pair of terminal asy

170 suggested that protonation of the C(5).A(16) wobble pair should shift C(5) toward the major groove an

171 l structure of a DNA duplex containing a T:G wobble pair shows similar structural changes imposed by

172 x G80), and disrupts a protonated C67 x A79 wobble pair that forms in the wild-type structure.

173 esulting in the formation of the U2506*G2583 wobble pair that was attributed to a catalytically inact

174 This novel mechanism enables the single wobble pair to dominantly determine the specificity of t

175 e isosteric, but pH-independent, G2450*U2063 wobble pair, and 50S subunits containing the mutations w

176 n the Watson-Crick pairs and 15 span the G:U wobble pair, including two interesting arrangements with

177 disrupts several tertiary contacts with the wobble pair, the assignment of A2450 as the active site

178 he DNAzyme confirm the importance of the G*T wobble pair, the two loops and the intervening stem in m

179 d, a pK(a) of 8.0 is observed for the A(+).C wobble pair, which represents an especially large shift

180 base pair, while discriminating against U/G wobble pair.

181 form stem that contains a pH-sensitive A(+)C wobble pair.

182 en the geometries of rF:G and the native U:G wobble pair.

183 y the 5'UAGG/3'GGAU loop adopts canonical UG wobble pairing (cis Watson-Crick/Watson-Crick), with AG

184 The wobble pairing and disorder of the Tg.G mismatch correla

185 Crystal structures reveal wobble pairing between C and O(6)-BzG.

186 cted to the complementary base plus a single wobble pairing for amino acids with twofold degenerate c

187 ut the glycosyl bond and forms a less stable wobble pairing interaction with guanine.

188 The U/G wobble pairing is ubiquitous in RNA, especially in non-c

189 G:T and A:C wobble pairing leads to a high error rate, but the modif

190 dified nucleoside 2-thiothymidine suppresses wobble pairing.

191 y crystallographic studies, which show that "wobble" pairing occurs between C and O6-MeG.

192 We believe that wobble-pairing between T and G is responsible for misinc

193 cal/mol on eight stacked pairs involving G-U wobble pairs and 0.99 kcal/mol on seven stacked pairs in

194 a identify a set of mutations, including G-U wobble pairs and nucleotide mismatches in the 5' hairpin

195 ut for both the protonated and nonprotonated wobble pairs at C(5).A(16).

196 This study suggests that protonated A(+).C wobble pairs exist in DNA under biologically relevant co

197 racyclines preferentially bind within the UG wobble pairs flanking an asymmetrically bulged C-residue

198 Among protonated mismatches, A(+).C wobble pairs form near physiological pH and have relativ

199 d ionic strength on pK(a) shifting in A(+).C wobble pairs in DNA.

200 search for exact matches while including G-U wobble pairs or employ simplified energy models, we pres

201 stable structure consisting of conserved UG wobble pairs, a folded 2X2 (GU/UA) internal loop, a UU b

202 Two wobble pairs, A2453-C2499 and A2450-C2063, have been pro

203 DNA splints, splints with G:U wobble pairs, and splints with G to I (Inosine) substitu

204 ismatches, including nearly isoenergetic RNA wobble pairs, can be efficiently rejected with discrimin

205 ino, AU reverse Hoogsteen, and the GU and AC wobble pairs.

206 stabilize U:A base pairs and destabilize U:G wobble pairs.

207 n to stabilize U:A pairs and destabilize U:G wobble pairs.

208 ryotes) that contain an adenosine (A) at the wobble position (position 34).

209 tion at the 2'-hydroxylribosyl moiety in the wobble position (Um34) of Sec tRNA([Ser]Sec), and conseq

210 ional modification, 5-formylcytidine, at the wobble position 34 (f(5)C(34)), and a cytidine substitut

211 e post-transcriptional modifications at tRNA wobble position 34 and purine 37, 3'-adjacent to the ant

212 e-5-oxyacetic acid (cmo (5)U 34) is found at wobble position 34 in a single isoaccepting tRNA species

213 e show that the complete modification at the wobble position 34 is 5-carboxyaminomethyl-2-thiouridine

214 Post-transcriptional modifications at tRNA's wobble position 34, especially modifications of uridine

215 odified 2-thiouridine (s(2)U) derivatives at wobble position 34.

216 n usage displays the expected GC bias in the wobble position and is consistent with a highly acidic p

217 e Elongator complex modifies uridines in the wobble position and is highly conserved in eukaryotes.

218 Modifications at the anticodon's wobble position are required for recognition of rarely u

219 y Shine-Dalgarno sequences, deprioritize the wobble position in each codon, and group codon synonyms

220 In most organisms, the C34 wobble position in these tRNA(Ile) precursors is rapidly

221 Queuosine (Q) was discovered in the wobble position of a transfer RNA (tRNA) 47 years ago, y

222 Here, we show (i) that unlike U34 at the wobble position of all B. subtilis tRNAs of known sequen

223 The 2-thiouridine (s(2)U) at the wobble position of certain bacterial and eukaryotic tRNA

224 methylcarboxymethyl uridine (mcm(5)U) at the wobble position of certain tRNAs, a critical anticodon l

225 on of the modified base queuine (Q) into the wobble position of certain tRNAs.

226 ce was also noted when only mutations in the wobble position of degenerate codons were considered.

227 The formation of inosine at the wobble position of eukaryotic tRNAs is an essential modi

228 inomethyluridine (taum(5)U) in the anticodon wobble position of five mitochondrial tRNAs.

229 uanosine nucleoside located in the anticodon wobble position of four amino acid-specific tRNAs.

230 droxyuridine into 5-oxyacetyl uridine at the wobble position of multiple tRNAs in Gram-negative bacte

231 is almost universally found in the anticodon wobble position of specific tRNAs.

232 hat is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containin

233 enouridine (mnm(5)se(2)U), is located at the wobble position of the anticodons of tRNA(Lys), tRNA(Glu

234 lly broad inosine-adenosine base pair at the wobble position of the codon cannot be maintained simult

235 terferes with the eRF1 decoding of the third/wobble position of the stop codon set in the unfavorable

236 Uridine 34 (U34) at the wobble position of the tRNA anticodon is post-transcript

237 ological importance of A-to-I editing at the wobble position of tRNA.

238 In such a strain, C34 at the wobble position of tRNA2(Ile) is expected to remain unmo

239 a modified C (lysidine or agmatidine) at the wobble position of tRNA2(Ile) to base pair specifically

240 l deamination of adenosine to inosine at the wobble position of tRNAs and is necessary to permit a si

241 Enzyme-mediated modifications at the wobble position of tRNAs are essential for the translati

242 e deamination of adenosine to inosine at the wobble position of tRNAs.

243 ng in formation of 5-oxyacetyluridine at the wobble position of tRNAs.

244 modification occurs in tRNAs from a G in the wobble position to Queuosine that changes optimal bindin

245 Sec) by methylation of the nucleoside in the wobble position was repressed.

246 C; (c) an arginine tRNA with Inosine in the wobble position which reads CGU, CGC, and CGA bypasses m

247 by the nucleotide flexibility of their codon-wobble position(s).

248 he modifications that occur at the first, or wobble position, of tRNA's anticodon and those 3'-adjace

249 the modified nucleotide queuosine (Q) at the wobble position, thereby preventing protein synthesis an

250 on of tRNA(Leu(CAA)) containing m(5)C at the wobble position, which causes selective translation of m

251 ional modification, 5-formylcytidine, at the wobble position-34 (f(5)C(34)).

252 f cytidine, 5-formylcytidine (f(5)C), at the wobble position-34 of human mitochondrial tRNA(f5CAU)(Me

253 rs in the absence of modifications at either wobble position-34 or the conserved purine-37, 3'-adjace

254 ypermodified nucleoside queuosine (Q) at the wobble position.

255 ducing mature tRNAs without adenosine at the wobble position.

256 e inosine tRNA modification in the anticodon wobble position.

257 nate tRNAs with a mismatch only at the third/wobble position.

258 ifications to uridine in the tRNA anticodon 'wobble' position in both yeast and higher eukaryotes.

259 w that modified nucleosides at the first, or wobble, position of the anticodon and 3'-adjacent to the

260 Wobble positions are inherently unselected since regardl

261 dified RNA base that replaces guanine in the wobble positions of 5'-GUN-3' tRNA molecules.

262 ine (Q) is a hypermodified base found in the wobble positions of tRNA Asp, Asn, His, and Tyr from bac

263 fication of certain tRNAs in their anticodon wobble positions with queuine.

264 r (3) mismatches at four or more consecutive wobble positions.

265 However, this G x A pair is flanked by G x U wobbles, rather than an unpaired wedge.

266 Because wobble rules preclude compensation for loss of tRNA(Lys4

267 discrimination against the formation of the wobble (Se)T/G base pair.

268 tion algorithms, are identified by the codon-wobble signatures of invariant amino acids.

269 structural and kinetic characterization of a wobble-specific deaminase.

270 The existence of the wobble state may be important for actin dynamics in cell

271 posal that G.fC and G.caC base pairs adopt a wobble structure that is recognized by TDG.

272 upon dsRNA binding and that canonical or GU-wobble substitutions produce dsRNA mutants that retain e

273 The internal AH(+).C wobble system further allowed us to parse energetic cont

274 0 self-complementary RNA duplexes containing wobble terminal base pairs with all possible 3' single a

275 pecially modifications of uridine 34, enable wobble to occur.

276 ble anticodons indicating widespread use of 'wobble' tRNAs.

277 The S6G.T structures exhibit a wobble-type base pairing at the lesion site, with thymin

278 shows that the CACG tetraloop is closed by a wobble U.G base pair.

279 the standard Watson-Crick (C:G and U:A) and wobble U:G conformations, an analysis of the base-pair t

280 elongation through tRNA modifications at the wobble (U(34)) position(5,6).

281 lical stem capped by a UACG tetraloop with a wobble UG closing base pair.

282 odifications of transfer RNAs (tRNAs) at the wobble uridine 34 (U34) base are highly conserved and co

283 sic region in Elp1 may be essential for tRNA wobble uridine modification by acting as tRNA binding mo

284 egulation, ubiquitination and cytosolic tRNA wobble uridine modification via 5-methoxycarbonylmethyl-

285 s essential for Elongator's function in tRNA wobble uridine modification.

286 on of translation elongation factor eEF2 and wobble uridine modifications of tRNAs.

287 oxycarbonylmethylation, respectively, of the wobble uridine of cytoplasmic (tK(UUU)), (tQ(UUG)), and

288 y directly controls the thiolation status of wobble-uridine (U34) nucleotides present on lysine, glut

289 chemical modifications are introduced at the wobble uridines at position 34 in transfer RNAs (tRNAs),

290 M1 pathway responsible for the thiolation of wobble uridines in cytoplasmic tRNAs tK(UUU), tQ(UUG) an

291 fine-tuned its specificity to correlate with wobble versus nonwobble positions across that sequence a

292 the actin filament, and bound CP is able to wobble when attached only via its mobile beta-subunit te

293 stability, and disfavored by external A(+).C wobbles, which have high folding cooperativities but mak

294 pK(a) shifting is favored by internal A(+).C wobbles, which have low cooperativities of folding and m

295 of the hind legs with a slight side-to-side wobble while walking.

296 g(2)(+) ion, we replaced the G25.U20 reverse wobble with an isosteric A25.C20 reverse wobble.

297 G.U wobbles with distinct widening have similar major groove

298 ly active, demonstrating that AAA+ unfoldase wobbling with respect to 20S is not required for functio

299 g principal order parameter Szz for overall "wobble" with respect to the membrane normal (molecular z

300 Wobble, with D(perpendicular)= 1-2 x 10(8) s(-1), is the