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1 express HbS after transfer of a 240-kb betas yeast artificial chromosome.
2 a 27-kb region around the Hoxc8 gene from a yeast artificial chromosome.
3 insert cloning systems such as bacterial and yeast artificial chromosomes.
4 maps for large genomic clones, possibly even yeast artificial chromosomes.
5 nel of rodent-human somatic cell hybrids and yeast artificial chromosomes.
6 s and identifying actin gene locations among yeast artificial chromosomes.
7 such as bacteriophage P1 clones, cosmids, or yeast artificial chromosomes.
8 flanking markers are contained in a 1,240-kb yeast artificial chromosome, a region small enough to pr
10 globin locus residing on a 248-kb beta-locus yeast artificial chromosome and analyzed its function in
13 n and the entire beta-globin gene locus in a yeast artificial chromosome and in 2 globin promoter-rep
14 ing this motif in a 248 kb beta-globin locus yeast artificial chromosome and measuring the activity o
15 cts or large, almost authentic, transloci on yeast artificial chromosomes and undergo B-cell-specific
16 'HS2 was recombined into a beta-globin locus yeast artificial chromosome, and transgenic mice were pr
17 al chromosome showed that it was in the same yeast artificial chromosome as the gene encoding liver g
18 irst to map the MEN1 region physically using yeast artificial chromosome, bacterial artificial chromo
19 ation of fluorescence in situ hybridization, yeast artificial chromosome, bacterial artificial chromo
20 ped in Xp22.2 by Southern blot analysis on a yeast artificial chromosome/bacterial artificial chromos
22 as found to be refractory to cloning in YAC (yeast artificial chromosome)-based vectors, a P1 artific
23 previous high level expression results with yeast artificial chromosome-based mouse Igkappa transgen
26 eleted upon V kappa-J kappa joining, we used yeast artificial chromosome-based techniques to fuse dis
29 with mice carrying a human beta-globin locus yeast artificial chromosome (beta YAC), and analyzed glo
30 ent of 5'HS4 from a 248 kb beta-globin locus yeast artificial chromosome (beta-YAC) and analyzed glob
31 A promoter interactions in beta-globin locus yeast artificial chromosome (beta-YAC) bone marrow cells
32 these possibilities, human beta-globin locus yeast artificial chromosome (beta-YAC) lines were produc
33 of fetal hemoglobin human beta-globin locus yeast artificial chromosome (beta-YAC) mice, showing tha
34 in the context of a human beta-globin locus yeast artificial chromosome (beta-YAC) results in profou
35 tionally silenced in adult beta-globin locus yeast artificial chromosome (beta-YAC) transgenic mice,
36 in the context of a human beta-globin locus yeast artificial chromosome (betaYAC) and analyzed the e
38 age of YAC128 mice that are transgenic for a yeast artificial chromosome carrying human HTT with an e
39 s of a set of mice transgenic for a modified yeast artificial chromosome carrying the human PAX6 locu
40 tamine neurotoxicity, we introduced human AR yeast artificial chromosomes carrying either 20 or 100 C
42 of a human disease gene in the context of a yeast artificial chromosome clone containing endogenous
44 n of expanded CAG repeat motifs into a 250kb yeast artificial chromosome clone spanning the MJD1 locu
45 ht chain transgene is derived in part from a yeast artificial chromosome clone that includes nearly h
46 The psoriasin gene was present on a 380-kb yeast artificial chromosome clone that was previously ma
47 ntaining the Mep1b gene were obtained from a yeast artificial chromosome clone using polymerase chain
48 markers mi69 and ASB2, which is covered by a yeast artificial chromosome clone, CIC9E2, on chromosome
49 ized the human LMX1.1 gene to three CEPH "B" yeast artificial chromosome clones (907A11, 935B12, and
51 u hybridization mapping of the corresponding yeast artificial chromosome clones and sequence-tagged s
54 ntified three highly overlapping nonchimeric yeast artificial chromosome clones containing the apex o
55 is gene were isolated by hybrid capture with yeast artificial chromosome clones covering the deletion
59 ted a topologic map of the 9p21 region using yeast artificial chromosome clones, pulse-field gel elec
63 , the single transgenic made with the 560 kb yeast artificial chromosome construct containing the tTA
64 rm in HT1080 cells after transfecting linear yeast artificial chromosome constructs minimally contain
65 e expression, mutant human beta-globin locus yeast artificial chromosome constructs were used, each h
66 Treatment of transgenic mice expressing a yeast artificial chromosome containing 128 CAG repeats (
70 have recently shown that Fmr1KO mice with a yeast artificial chromosome containing the human FMR1 ge
71 generated a line of transgenic mice using a yeast artificial chromosome containing the Ret mutation
72 netic and physical mapping of scm identified yeast artificial chromosomes containing an exon of mdab1
73 ransgenic mice were generated by transfer of yeast artificial chromosomes containing the entire human
74 Here, we report the construction of a 20-Mb yeast artificial chromosome contig and a high-resolution
75 er of cancer cell lines after isolation of a yeast artificial chromosome contig and development of ST
77 known mammalian ARF genes, to a well-defined yeast artificial chromosome contig on human chromosome 7
78 ned approximately 2-3 Mb, as determined by a yeast artificial chromosome contig reported to cover thi
83 However, it is difficult to purify intact yeast artificial chromosome DNA at a concentration suffi
84 tlc1-human allele, and sequencing of rescued yeast artificial chromosome ends has verified the additi
85 re identical to those of C. elegans cosmids, yeast artificial chromosomes, expressed sequence tags, o
86 Chinese hamster ovary cells containing the yeast artificial chromosome F136C5 (alphaYAC) respond to
88 etional analysis across the 450-kb region by yeast-artificial-chromosome fragmentation and phage P1 c
92 f sequence conservation, using as template a yeast artificial chromosome known to contain both interl
94 nic mouse lines carrying a beta-globin locus yeast artificial chromosome lacking the LCR to determine
96 urospora crassa was cloned previously from a yeast artificial chromosome library and was found to be
97 tude du Polymorphisme Humain megabase-insert yeast artificial chromosome library, which contains info
99 and screening of a chromosome-specific YAC (yeast artificial chromosome) library revealed that the g
100 CR-based screening to construct a continuous yeast artificial chromosome map covering >20 mb in human
101 enetic maps, expressed sequence tags (ESTs), yeast artificial chromosome maps, bacterial artificial c
102 ession in human erythroid cells, beta-globin yeast artificial chromosome mice, and sickle cell diseas
103 The map comprises a contig of 14 overlapping yeast artificial chromosomes onto which we positioned 25
104 recombination in yeast, either as part of a yeast artificial chromosome or in a yeast-E. coli shuttl
105 iled physical map of nearly 2 Mb, containing yeast artificial chromosomes, P1-derived artificial chro
106 Fluorescence in situ hybridization using a yeast artificial chromosome physically mapped AVPR1A to
111 e mapped six novel ESTs to a 15-Mb contig of yeast artificial chromosomes spanning the critical regio
114 fingerprinting and previously characterized yeast artificial chromosomes subcloned into cosmids and
116 in gene 3' enhancer, by deleting them from a yeast artificial chromosome that spans the human beta-gl
118 n any of the overlapping parental BACs and a yeast artificial chromosome that were originally tested
119 nd by hybridization to two overlapping human yeast artificial chromosomes that are located in 4q22.
120 roblasts transformed with multiple copies of yeast artificial chromosomes that contain the full-lengt
121 f genomic DNA from higher eukaryotes such as yeast artificial chromosomes, the delitto perfetto strat
124 -in, pop-out" gene targeting in a human apoB yeast artificial chromosome to introduce nonsense mutati
125 d 3' end of the gene were subcloned from the yeast artificial chromosomes to enable untranscribed DNA
129 Characterization of the largest element in yeast artificial chromosome transgenic mice revealed it
134 -p21, composed of 205 loci, connected by 480 yeast artificial chromosomes, with average interlocus di
135 e chain reaction the localization of ATIC to yeast artificial chromosome (YAC) 914E7 previously repor
136 ion and characterization of newly identified yeast artificial chromosome (YAC) and bacterial artifici
137 pulsed-field gel electrophoresis (PFGE), and yeast artificial chromosome (YAC) and bacterial artifici
141 eplaced with the PyE within the context of a yeast artificial chromosome (YAC) bearing the whole locu
142 thin their life span, we decided to obtain a yeast artificial chromosome (YAC) carrying the AR gene a
143 ed the core elements within the context of a yeast artificial chromosome (YAC) carrying the entire lo
145 a new hybrid vector, pTARBAC1, containing a yeast artificial chromosome (YAC) cassette (a yeast sele
146 by direct complementary DNA selection from a yeast artificial chromosome (YAC) clone containing a 650
149 kers from the region did not identify intact yeast artificial chromosome (YAC) clones after screening
150 n myeloid hematopoietic cells, we introduced yeast artificial chromosome (YAC) clones and derivatives
151 cosmid clones to three partially overlapping yeast artificial chromosome (YAC) clones and determined
152 e sequence length polymorphisms (SSLPs) from yeast artificial chromosome (YAC) clones containing regi
154 ll hybrids, and five independent overlapping yeast artificial chromosome (YAC) clones containing the
155 in situ hybridization with a panel of mapped yeast artificial chromosome (YAC) clones from 7q to exam
157 s on human metaphase chromosomes of over 950 yeast artificial chromosome (YAC) clones from the CEPH l
158 ysical map of P1 (PAC), bacterial (BAC), and yeast artificial chromosome (YAC) clones of this interva
159 The physical map consists of a contig of 420 yeast artificial chromosome (YAC) clones ordered with re
160 ites (STSs) within the region, a total of 21 yeast artificial chromosome (YAC) clones were isolated a
162 have integrated into 721.174 two overlapping yeast artificial chromosome (YAC) clones which cover the
166 re constructed from multimegabase contigs of yeast artificial chromosome (YAC) clones; 2) they are in
167 ping data from reduced radiation 8p hybrids, yeast artificial chromosome (YAC) co-localisation of mar
169 previously reported the generation of human yeast artificial chromosome (YAC) constructs encompassin
170 bout 50 kb from the IgH intron enhancer in a yeast artificial chromosome (YAC) containing a 220 kb re
171 rand break (DSB) within a 360-kb dispensable yeast artificial chromosome (YAC) containing human DNA (
172 y studying the transcriptional activity of a yeast artificial chromosome (YAC) containing Igf2 and H1
173 ferred by this element by deleting it from a yeast artificial chromosome (YAC) containing the entire
174 roplasts that results in the generation of a yeast artificial chromosome (YAC) containing the gene of
176 nic mice by pronuclear injection of a 380 kb yeast artificial chromosome (YAC) containing the human P
177 a 6-kbp promoter fragment nor even a 120-kbp yeast artificial chromosome (YAC) containing the whole G
178 mouse chromosome 1 and the construction of a yeast artificial chromosome (YAC) contig across this reg
179 ning efforts to identify the RMD-1 TSG(s), a yeast artificial chromosome (YAC) contig consisting of 1
184 The telomeric end of the contig overlaps a yeast artificial chromosome (YAC) contig from Xq24-q26 a
186 cute insulin response, we have constructed a yeast artificial chromosome (YAC) contig map that spans
187 in head and neck squamous cell carcinoma, a yeast artificial chromosome (YAC) contig spanning the en
188 lable regional DNA markers, we constructed a yeast artificial chromosome (YAC) contig that contained
192 f other cereal genomes, has resulted in rice yeast artificial chromosome (YAC) contigs spanning parts
193 ability of foreign telomeres to complement a yeast artificial chromosome (YAC) deficient by one telom
194 available that allow the transfer of intact yeast artificial chromosome (YAC) DNA into transgenic mi
195 nd approach, MACs were formed from a defined yeast artificial chromosome (YAC) DNA molecule containin
201 d-tagged sites (STSs) against a large-insert yeast artificial chromosome (YAC) library and then integ
202 struction and characterization of an arrayed yeast artificial chromosome (YAC) library for the rat ge
204 gene members on this cluster, a human CEPH B yeast artificial chromosome (YAC) library was screened b
206 curately in transgenic mice that carry large yeast artificial chromosome (YAC) or ligated cosmid cons
207 l artificial chromosome (BAC) contigs from a yeast artificial chromosome (YAC) physical map is descri
210 omosome 13q12 region by cytogenetic mapping, yeast artificial chromosome (YAC) pool screening, and ra
211 lting BAC contigs and oriented them within a yeast artificial chromosome (YAC) scaffold by observing
213 ave focused on strategies that would utilize yeast artificial chromosome (YAC) technology, thus permi
214 able transfection of an approximately 620-kb yeast artificial chromosome (YAC) that carried the entir
215 We then compared expression from a 310 kb yeast artificial chromosome (YAC) that contains the enti
216 ome (HAC) vector was constructed from a 1-Mb yeast artificial chromosome (YAC) that was selected base
219 herapeutic approaches to fragile X syndrome, yeast artificial chromosome (YAC) transgenic mice were g
221 ism and A beta deposition in a genomic-based yeast artificial chromosome (YAC) transgenic mouse model
222 ucted a YAC/STS contig map by initiating two yeast artificial chromosome (YAC) walks from the markers
224 Here we describe transgenic mice carrying a yeast artificial chromosome (YAC) with the intact human
225 transgenic mouse line bearing a 662-kb Gata3 yeast artificial chromosome (YAC), and these animals (te
228 wing introduction of the human APP gene in a yeast artificial chromosome (YAC), we examined the effec
229 letion critical region was assembled using a yeast artificial chromosome (YAC)-based isolation and bi
230 udy was to assess the feasibility of using a yeast artificial chromosome (YAC)-based reporter gene co
236 earlier study, Fmr1 knockout mice carrying a yeast-artificial chromosome (YAC) transgene that over-ex
237 ecently we described the identification of a yeast artificial chromosome, YAC 927G4, that spans a tra
239 for physical mapping consists of contigs of yeast artificial chromosome (YACs), large vectors that a
240 ave introduced two different CFTR-containing yeast artificial chromosomes (YACs) (320 and 620 kb) int
241 een mapped at seven-fold average coverage in yeast artificial chromosomes (YACs) and 100 kb inter-seq
243 a large human locus, overlapping regions on yeast artificial chromosomes (YACs) and cosmids were lin
245 demonstrate here that human genes present on yeast artificial chromosomes (YACs) are transcribed in y
247 rt the isolation and characterization of two yeast artificial chromosomes (YACs) bearing the murine G
248 ment of a system for shuttling DNA cloned as yeast artificial chromosomes (YACs) between yeast and ma
249 stability by generating transgenic mice with yeast artificial chromosomes (YACs) carrying AR CAG repe
250 ouse antibody production and engineered with yeast artificial chromosomes (YACs) containing different
251 Nearly all the M1-selected transformants had yeast artificial chromosomes (YACs) containing human DNA
252 accurately a variety of linear and circular yeast artificial chromosomes (YACs) containing human DNA
254 n can be used to assess gene expression from yeast artificial chromosomes (YACs) containing the 230 k
255 beta production in vivo, we have introduced yeast artificial chromosomes (YACs) containing the entir
256 We propose a new method for segregation of yeast artificial chromosomes (YACs) from endogenous yeas
258 ve isolation of human DNAs as large circular yeast artificial chromosomes (YACs) from two rodent/huma
259 ransgenesis with large DNA molecules such as yeast artificial chromosomes (YACs) has an advantage ove
260 simple and rapid assay for GCRs, exploiting yeast artificial chromosomes (YACs) in Saccharomyces cer
261 ic stem (ES) cell lines containing different yeast artificial chromosomes (YACs) integrated into the
262 en established to convert pYAC4-based linear yeast artificial chromosomes (YACs) into circular chromo
263 describe a modified protocol for introducing yeast artificial chromosomes (YACs) into murine embryoni
264 expressed sequence tags (ESTs) identified 33 yeast artificial chromosomes (YACs) or P1 clones, which
265 (FISH) was then performed using a series of yeast artificial chromosomes (YACs) ordered in the CDR,
267 ange restriction mapping was performed using yeast artificial chromosomes (YACs) spanning approximate
268 ndidate interval for NP-C, three overlapping yeast artificial chromosomes (YACs) spanning the 1 centi
270 ective isolations of the entire HPRT gene as yeast artificial chromosomes (YACs) that extended from t
271 r transgenic mice, containing four different yeast artificial chromosomes (YACs) that together cover
273 some sequences in the form of large circular yeast artificial chromosomes (YACs) up to 170 kb in size
274 isolated the region between these markers in yeast artificial chromosomes (YACs) using a combination
275 murine cells, a transgenesis system based on yeast artificial chromosomes (YACs) was established for
276 equence-tagged site (STS) content mapping in yeast artificial chromosomes (YACs) was used to cover th
278 bility of human DNA contained in dispensable yeast artificial chromosomes (YACs) within the yeast Sac
279 The map comprises a contig of 84 overlapping yeast artificial chromosomes (YACs), P1 artificial chrom