ライフサイエンスコーパス: zygote

1 igh efficiency (0.62%-5.13% of microinjected zygotes).

2 ustly generate different cell types from one zygote.

3 rm coexist as separate haploid nuclei in the zygote.

4 contractility in the Caenorhabditis elegans zygote.

5 on of centrosomes after fertilization in the zygote.

6 marker of the developmental potential of the zygote.

7 ognize each other and fuse to form a diploid zygote.

8 fferent cell lineages during cleavage of the zygote.

9 a failure to form astral microtubules in the zygote.

10 maintain cortical polarity in the C. elegans zygote.

11 al requirement in the Caenorhabditis elegans zygote.

12 petuated from oogenesis or reacquired by the zygote.

13 t in the oocyte or imprint protection in the zygote.

14 ferent cell types have emerged from the same zygote.

15 nomes in an individual derived from a single zygote.

16 ted role for rRNA transcription in the mouse zygote.

17 arallels with polarization of the C. elegans zygote.

18 ired for two haploid gamete nuclei to form a zygote.

19 oth genetic material and centriole(s) to the zygote.

20 ntee a normal bipolar mitotic spindle in the zygote.

21 contributed by the sperm are present in the zygote.

22 ust recognize one another and fuse to form a zygote.

23 f every cell's ancestry back to the founding zygote.

24 ession program and regain totipotency in the zygote.

25 ity gradients and polarity in the C. elegans zygote.

26 much higher in developing germ cells than in zygotes.

27 injection of CRISPR/Cas9 reagents into mouse zygotes.

28 s completion and formation of normal diploid zygotes.

29 9 DNA/mRNA and single-guide RNA (sgRNA) into zygotes.

30 godeoxynucleotide (ssODN) complex into mouse zygotes.

31 ell-based system and microinjection of mouse zygotes.

32 ernal and maternal nuclei within single-cell zygotes.

33 hort stretches of DNA sequences into porcine zygotes.

34 bonucleoprotein (ctRNP) complexes into mouse zygotes.

35 single-stranded repair template into porcine zygotes.

36 and display distinct compartmentalization in zygotes.

37 after fertilization in pig and rhesus monkey zygotes.

38 re not well tolerated by normally fertilized zygotes.

39 d gametic cells of each mating type and from zygotes.

40 lowed by chloroplast DNA hypermethylation in zygotes.

41 ng for polygenic disease modeling in primate zygotes.

42 abrogated paternal DNA hydroxymethylation in zygotes.

43 drial-encoded mCherry was microinjected into zygotes.

44 duction of heteroplasmy shift in oocytes and zygotes.

45 e transposase, into the cytoplasm of porcine zygotes.

46 ting in heritable survivors from 1.2 million zygotes.

47 t3-depleted and/or DNA replication-inhibited zygotes.

48 ndle and ensures symmetric division of mouse zygotes.

49 favorable for the survival of the resulting zygotes.

50 xtra centrosomes are detected in a subset of zygotes.

51 zation overlaps with the array in polarizing zygotes.

52 mechanisms of H3K27me2 and H3K27me3 in mouse zygotes.

53 dine deaminase (AID; Aicda) directly in BXSB zygotes.

54 d for the establishment of H3K27me3 in mouse zygotes.

55 donor template or an HMEJ template in bovine zygotes.

56 gential pronuclear alignment compared to IVF zygotes.

57 undergo heterologous fusions in gsm1 or gsp1 zygotes.

58 usions required for the genetic unity of the zygotes.

59 f nucleolar-like bodies compared with normal zygotes.

60 tion efficiency as opposed to MII oocytes or zygotes.

61 e the origin of the first centrosomes in the zygote [2-4].

62 s the pre-existence of TADs and loops in the zygote(5,11).

63 (75%); however, a significant portion of ST zygotes (52%) showed abnormal fertilization as determine

64 etes are reprogrammed to create a totipotent zygote, a process that involves de novo establishment of

65 red indirectly for nuclear fusion; sey1Delta zygotes accumulate ER at the zone of cell fusion, causin

66 s provide important insights into gamete and zygote activity in plants, and our RNA-seq transcriptome

67 f both the father and mother, but not of the zygote, affects embryonic viability and growth during ge

68 Simultaneous gene editing in zygotes affords an efficient approach for producing mice

69 interactions that can be transmitted to the zygote after fertilization.

70 Mild hypothermic (30 degrees C) culture of zygotes after microinjection increased HDR efficiency fo

71 This misregulation of 5hmec in zygotes also affected the level of NANOG expression in t

72 cript abundance of TET3 was high only at the zygote and 2-cell stage.

73 r maternal chromosomes, respectively, in the zygote and 2-cell stages.

74 ial roles in remodeling the epigenome in the zygote and cleavage stage embryos, when the maternal gen

75 rade maternally deposited transcripts in the zygote and developmental arrest.

76 ace during a window(s) of opportunity in the zygote and early embryo; (ii) there is no statistical va

77 o occur gradually, with the initial steps of zygote and embryo development being primarily maternally

78 ability to directly modify the genome in the zygote and generate edited animals is highly desirable.

79 OT1L deposition and H3K79 methylation in the zygote and in the preimplantation stage embryo is dispen

80 amete-derived methylation to maintain in the zygote and preimplantation embryo at a time when much of

81 l cells of the female gametophyte and in the zygote and proliferating endosperm of the Arabidopsis (A

82 ametic functions, and their contributions to zygote and seed development.

83 sembled in gametes prior to formation of the zygote and then subsequently re-established as developme

84 nal pronuclei expanded in size in the mutant zygotes and contained a significantly increased number o

85 at the ROSA26 "safe harbor" locus in ferret zygotes and created transgenic animals expressing a dual

86 he surface of P. falciparum macrogametes and zygotes and effectively prevented parasites from develop

87 gene encoding OCT4 (POU5F1) in diploid human zygotes and found that blastocyst development was compro

88 th high SPTRX3 produced fewer two-pronuclear zygotes and had a reduced pregnancy rate (19.2% pregnant

89 , is present in the nucleus and cytoplasm of zygotes and has been associated with protecting the fema

90 cinity of sperm mitochondrial sheaths in the zygotes and increased in the embryos treated with protea

91 e two species and the viability of resulting zygotes and larvae.

92 ecific DNase I hypersensitive sites in mouse zygotes and morula embryos, and investigate the epigenet

93 cell fusion, how cells join together to form zygotes and multinucleated syncytia has remained a funda

94 berghei PIMMS2 is specifically expressed in zygotes and ookinetes and is localized on the ookinete s

95 Pfs25, an antigen expressed on malaria zygotes and ookinetes, is a leading transmission blockin

96 of Plasmodium falciparum including gametes, zygotes and ookinetes, is one of the primary targets for

97 ocalization pattern of Mcp5 in fission yeast zygotes and show by perturbation of phosphatidylinositol

98 ion is linked to asymmetric cell division in zygotes and stomatal lineages, which require integrated

99 Microinjection of the vectors into zygotes and transfer of the embryos to recipient animals

100 anscriptomic homeostasis in fertilized eggs, zygotes and two-cell embryos.

101 tact and enucleated metaphase and interphase zygotes and two-cell embryos.

102 the onset of ring assembly in the C. elegans zygote, and provide a framework for determining emergent

103 dataset constitutes 56 genetic loci, 17,887 zygotes, and 1718 live-born mice, of which only 15 (0.87

104 d RNA-seq transcriptome profiles of gametes, zygotes, and apical and basal daughter cells.

105 ned the dynamics of H3K9me2 changes in mouse zygotes, and investigated the regulatory mechanisms.

106 s, revealed single-cell chromatin domains in zygotes, and uncovered epigenetic memory of global chrom

107 by haploid microgametes, results in diploid zygotes, around which a protective wall develops, formin

108 Using the Caenorhabditis elegans zygote as a model, we find that the localization and act

109 In Plasmodium, meiosis occurs in diploid zygotes as they develop into haploid motile ookinetes in

110 of metazoa to reset centriole number in the zygote at fertilization.

111 ctive system limits the accessibility of the zygote at the 1-cell stage.

112 Zygotes at the 1-cell stage have been genetically modifi

113 The maternal supply of Cas9 to the zygote avoids the necessity to deliver the relatively la

114 PAR proteins, which specify polarity in the zygote, be poised to respond to the centrosome.

115 despread active and passive demethylation in zygotes before the first mitotic division.

116 Among normally fertilized ST zygotes, blastocyst development (62%) and embryonic stem

117 elivering signals not only to gametes or the zygote but also to tissues of the maternal reproductive

118 abnormal fertilizations leading to triploid zygotes, but also normally fertilized zygotes can sponta

119 or destroying mitochondria delivered to the zygote by the sperm [4-13].

120 oops and domains have been detected in mouse zygotes by single-nucleus Hi-C (snHi-C), but not bulk Hi

121 sponsible for the appearance of 5hmec in the zygotes by TET3.

122 Diminution of CDC6 level in mouse zygotes by two different methods results in accelerated

123 d macrogametes for the development of motile zygotes, called ookinetes, which invade and transverse t

124 oth TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs.

125 leavage embryos, but we also discovered that zygotes can spontaneously segregate entire parental geno

126 iploid zygotes, but also normally fertilized zygotes can spontaneously segregate entire parental geno

127 ising from DNA demethylation, which prevents zygotes carrying unrepaired lesions from entering mitosi

128 s can predict the percentages of success for zygote cleavage and embryo blastocyst formation.

129 e the transfer of nuclear DNA from an egg or zygote containing defective mitochondria to a correspond

130 designated as CRISPR RNP Electroporation of Zygotes (CRISPR-EZ), enables highly efficient and high-t

131 r envelope breakdown (NEBD), we find that in zygotes cyclin A2 remains stable for a significant perio

132 ctile dynamics in the Caenorhabditis elegans zygote cytokinetic ring.

133 chiral counter-rotating cortical flow in the zygote, depend on myosin activity, and can be altered th

134 We uncover that C. elegans zygotes depleted of the Aurora A kinase AIR-1 or lacking

135 While PBNT zygotes developed to blastocysts less frequently (42%) t

136 el plant system, we determined the timing of zygote development and generated RNA-seq transcriptome p

137 tocytes in preparation for fertilization and zygote development, coinciding with parasite transition

138 erstand the roles that GSM1 and GSP1 play in zygote development, we used gsm1 and gsp1 mutants and ex

139 ELL1-like transcription factor essential for zygote development.

140 ought that transcription is not required for zygote development.

141 C oxidation and in the structural aspects of zygote development.

142 sphorylation pathways involved in sexual and zygote differentiation.

143 nterior-posterior polarity in the C. elegans zygote, diffusing from centrosomes to the overlying cort

144 The Arabidopsis (Arabidopsis thaliana) zygote divides to produce an embryonic lineage and an ex

145 ective delivery of CRISPR/Cas9 machinery via zygote electroporation as an alternative to the conventi

146 the transcriptomes of viable and non-viable zygotes, especially in expression of genes important for

147 onfocal imaging of live starfish oocytes and zygotes expressing markers of microtubule nucleating act

148 we report that in the Caenorhabditis elegans zygote, feedback between active RhoA and myosin induces

149 bly of the keratin network between asters in zygotes fixed before and during 1(st) cytokinesis.

150 criptomes of time-staged isogenic and hybrid zygotes following fertilization.

151 reprogramming of the epigenome to prime the zygote for totipotency.

152 and our single-AAV platform to pre-implanted zygotes for streamlined generation of genome-edited mice

153 ocytes are enriched in proteins required for zygote formation and functions after fertilization; prot

154 In the sexual stages, zygote formation and initial ookinete differentiation pr

155 NA from the parental cells, did not mix upon zygote formation but remained segregated at the poles by

156 oss of activity were closely associated with zygote formation in mating-cell pairs, supporting a role

157 otic Oct4 suggest that it is dispensable for zygote formation, early cleavage and activation of Nanog

158 hat initiates DNA demethylation during early zygote formation, embryogenesis, and neuronal differenti

159 gy in livestock by enabling gene knockout in zygotes from any chosen mating.

160 d 627 genes that are specifically induced in zygotes; furthermore, these sex-related gene sets were e

161 By microinjecting tru-RGN RNAs into zygotes, FVII KO mice were generated with higher efficie

162 m tsRNA fractions from HFD males into normal zygotes generated metabolic disorders in the F1 offsprin

163 e RNAs (sgRNAs) targeting Tet1 and Tet2 into zygotes generated mice with biallelic mutations in both

164 The initially symmetric fucoid zygote generates a developmental axis that determines no

165 ation platform based on CRISPR-Cas9-mediated zygote genome editing and show enrichment of rat PSC-der

166 CRISPR-Cas9 mediated zygote genome editing enables high efficient production

167 When combined with efficient zygote genome editing technologies, xenogeneic human plu

168 etric division of the Caenorhabditis elegans zygote, germ (P) granules are disassembled in the anteri

169 During polarization of the C. elegans zygote, germline RNA granules, called P granules, assemb

170 Genetic mosaicism arises when a zygote harbors two or more distinct genotypes, typically

171 delivered as ribonucleoprotein (RNP) to the zygote has become a standard tool for the development of

172 protein and synthetic guide RNAs into mouse zygotes has been shown to facilitate gene disruption and

173 were identified from 50 ng of Xenopus laevis zygote homogenate, which is comparable with an offline s

174 flagella, an event that normally renders the zygotes immotile.

175 their roles in the symmetric division of the zygote in early mouse development.

176 and then to the future posterior side of the zygotes in a later phase of cytoplasmic reorganization,

177 important for the self-renewal of fertilized zygotes in Caenorhabditis elegans and neuroblasts in Dro

178 oporation to deliver the components into the zygotes in situ.

179 ces in gene expression to be resolved in the zygote, including pathways affecting chromatin configura

180 iptomic changes were observed in unicellular zygotes, including upregulation of S-phase genes, a char

181 ion at meiosis II, thereby ensuring that the zygote inherits the appropriate complement of chromosome

182 Out of 151 zygotes injected with circular transgene-containing plas

183 s during CRISPR/Cas9-mediated mutagenesis by zygote injection in mice.

184 i-allelic null mutations in the Tyr locus by zygote injection of two single-guide and Cas9 RNAs.

185 new route for genome engineering in pigs via zygote injection should greatly enhance applications in

186 current approaches with this method require zygote injection, making it difficult to assess the adul

187 ch 100% which implies that genome editing by zygote injections can facilitate the one-step generation

188 ssential for the development of a totipotent zygote into an embryo with defined cell lineages.

189 Genome-wide DNA "demethylation" in the zygote involves global TET3-mediated oxidation of 5-meth

190 e avian species, genetic modification of the zygote is difficult because its unique reproductive syst

191 This appearance of 5hmec in zygote is important for the expression of NANOG in the b

192 species, cryopreservation of the oocyte and zygote is not possible due to the large size and quantit

193 e early embryogenesis, and the genome of the zygote is only switched on later.

194 The formation of zygote is the beginning of mammalian life, and dynamic e

195 ing plants, the asymmetrical division of the zygote is the first hallmark of apical-basal polarity of

196 ition from differentiated cell to totipotent zygote is unknown.

197 disruption in mouse embryonic stem cells or zygotes is a conventional genetics approach to identify

198 r the introduction of large DNA sequences in zygotes is low.

199 The cleavage furrow in Xenopus zygotes is positioned by two large microtubule asters th

200 zation, as the cytoplasm of pronuclear-stage zygotes is reportedly inactive.

201 efficient, as treating an average of only 50 zygotes is sufficient to produce a correctly targeted al

202 tensively used to manipulate the germline in zygotes, its application in postnatal gene editing remai

203 e dynamics that drag the nucleus through the zygote; known as horsetail movement.

204 clear and mitotic events in nonhuman primate zygotes leading to the establishment of polarity are inv

205 ch occurs during the first cell cycle in the zygote, leading to embryos with non-mosaic restoration o

206 ng TALEN plasmids into rhesus and cynomolgus zygotes leads to effective gene editing of MECP2 with no

207 In ascidian zygotes, maternal determinants (mRNAs) are first transpo

208 ter offspring stress responsivity and, using zygote microinjection of the nine specific miRs, demonst

209 n induce targeted CpG methylation in mice by zygote microinjection, thereby demonstrating its potenti

210 similar degree of mosaicism when compared to zygote microinjection.

211 n transgenic technology such as isolation of zygotes, microinjection of NAs into them, and their subs

212 nts suggest that epigenetic silencing in the zygote might act predominantly through female-dependent

213 st as animals develop from a single cell-the zygote-multicellular rosettes of S. rosetta develop from

214 In gsm1 and gsp1 zygotes, no fusion was observed for the nucleus and chlo

215 Although two-celled D. dichotoma zygotes normally develop a rhizoid from one pole and a t

216 te that the global chromatin organization of zygote nuclei is fundamentally different from that of ot

217 activity when retained experimentally in the zygote of the radiate star Asterias forbesi.

218 TALEN-Agouti mRNAs injected into zygotes of brown FvB x C57BL/6 hybrid mice generated com

219 determined that multipolar divisions at the zygote or two-cell stage were associated with CCFs and g

220 rotubule asters during mitosis in C. elegans zygotes or HeLa cells, respectively.

221 ed methods are not applicable to oocytes and zygotes owing to a paucity of material.

222 In Caenorhabditis elegans zygotes, PAR-1 localizes to the posterior cortex under t

223 stoichiometry during Caenorhabditis elegans zygote polarization, which takes place in less than 20 m

224 hoA activity and pulsed contractility during zygote polarization.

225 n is SSP protein transiently produced in the zygote, presumably from paternally inherited transcripts

226 t these are newly transcribed in the A. suum zygote prior to pronuclear fusion.

227 It is present in murine zygotes prior to the maternal to the zygotic transition

228 arks can thus serve as an early biomarker of zygote quality in mouse model.

229 ortening the half-life of Cas9 in fertilized zygotes reduces mosaic mutations and increases its abili

230 Most Setd1b(Gdf9) cKO zygotes remained in the pronuclear stage and displayed p

231 nd regulation of epigenetic asymmetry in the zygote remains obscure.

232 perm tsRNAs from the F1-HFD male into normal zygotes reproduces obesogenic phenotypes and addictive-l

233 erior polarity in the Caenorhabditis elegans zygote requires two different processes: mechanical acti

234 The first cell of an animal (zygote) requires centrosomes that are assembled from pat

235 on of siRNA specific to PHLDA2 into one-cell zygotes resulted in a substantial increase in blastocyst

236 ion into Tyr heterozygous (B6CBAF1/JxFVB/NJ) zygotes resulted in the generation of numerous albinos a

237 Following microinjection of zygotes, resulting morphant larvae were scored for axial

238 clease and donor DNA microinjection into rat zygotes results in efficient and reproducible targeted d

239 When gsm1 and gsp1 zygotes resumed the mitotic cycle, their two nuclei fuse

240 cross an approximately 30-fold difference in zygote size.

241 as9 system in metaphase II (MII) oocytes and zygote stage embryos.

242 mally invasive mechanical measurement at the zygote stage.

243 , is required for mouse development past the zygote stage.

244 ilization experiments suggest that the maize zygote starts cell wall deposition within 30 seconds aft

245 s unaffected by the deletion of TDG from the zygote, suggesting the existence of other demethylation

246 vated levels of Amhr2 in two- and eight-cell zygotes, suggesting ectopic Tspo silencing before the mo

247 e bodies retained 5mC in the maternal mutant zygotes, suggesting that the pericentromeric heterochrom

248 Less than half of human zygotes survive to birth, primarily due to aneuploidies

249 In wild-type Caenorhabditis elegans zygotes, symmetry breaking during anterior-posterior axi

250 coding an oleosin-like protein (oleolike) in zygotes-tetrads and a transcript encoding oleosin in veg

251 results in the creation of mutations in the zygote that can influence the health and well-being of t

252 C oxidation was significantly reduced in the zygotes that carried maternal mutation of either the Ehm

253 spark profiles revealed that parthenotes and zygotes that developed into blastocysts released more zi

254 etric division of the Caenorhabditis elegans zygote, the PAR proteins orchestrate the segregation of

255 ng the asymmetric division of the C. elegans zygote, the RNA-binding protein MEX-5 forms an anterior-

256 vegetal-pole proteomic asymmetry in the frog zygote, the technology was applied to uncover proteomic

257 In the C. elegans zygote, the timing and geometry of polarization rely on

258 delivery of targeting components directly to zygotes, these strategies are quite inefficient.

259 omesoderm specification in the sea star from zygote through gastrulation that corresponds to the GRN

260 onic development, going from a single-celled zygote to a complex multicellular adult.

261 eaching of those religions that consider the zygote to be a human person with an immortal soul.

262 ly transcriptionally quiescent, allowing the zygote to be reprogrammed into a totipotent state.

263 olites in whole zebrafish from the period of zygote to free-swimming larvae 6 days postfertilization

264 tein complex can be microinjected into mouse zygotes to edit endogenous sites with the 5'-YG-3' PAM,

265 production of mature gametes and fertilized zygotes to favorable nutritional conditions improves rep

266 nucleases and donor DNA microinjection into zygotes to generate HDR-modified rats with large new seq

267 s9 DNA/RNA and single guide RNA (sgRNA) into zygotes to generate modified animals in one step.

268 irected repair template into NOD single-cell zygotes to introduce the Ptpn22(R619W) mutation to its e

269 lease (ZFN)-encoding mRNA or DNA into bovine zygotes to verify cleavage activity at their target site

270 effector nucleases (TALEN) mRNAs into mouse zygotes transferred into foster mothers efficiently gene

271 is uniquely reorganized during the oocyte-to-zygote transition in mice and is distinct in paternal an

272 of the paternal genome during the oocyte-to-zygote transition.

273 nce of H3K9me2 in the male pronucleus of the zygote treated with cycloheximide.

274 mere lengths progressively increase from the zygote, two-cell to four-cell embryo.

275 ight-dependent germination, during which the zygote undergoes meiosis that gives rise to four vegetat

276 the germination of the alga, under which the zygote undergoes meiosis, in a positive manner, similar

277 The formation of a zygote via the fusion of an egg and sperm cell and its s

278 Here we report that zygote viscoelastic properties can predict blastocyst fo

279 While the first division in wild-type zygote was asymmetrical, a nearly symmetrical division o

280 CRISPR/Cas9 genomic editing in murine zygotes was used to generate knockin mice with a catalyt

281 the newly fertilized Caenorhabditis elegans zygote, we show that the mitotic Polo-like kinase PLK-1

282 Using symmetrically dividing sea urchin zygotes, we generated cortical domains of magnetic parti

283 f two base editors and two sgRNAs into mouse zygotes, we introduced C.G-to-T.A transitions into two c

284 ed donor oligonucleotides (ssODN) into mouse zygotes, we introduced defined genomic modifications in

285 tudies involving abnormally fertilized human zygotes were not well tolerated by normally fertilized z

286 fully fertile hermaphrodite plant to produce zygotes when self-pollinated'--a definition that is neut

287 ube (PT) fuses with the egg cell to form the zygote, whereas the second unites with the central cell

288 -editing molecules into the cytoplasm of pig zygotes, which generated the anephrogenic phenotype.

289 ernal genome is actively demethylated in the zygote while the maternal genome undergoes subsequent pa

290 ctive mitochondria to a corresponding egg or zygote with normal mitochondria.

291 after fertilization to produce a totipotent zygote with the potential to generate a new organism.

292 ver Cas9/sgRNA ribonucleoproteins into mouse zygotes with 100% efficiency for in vivo genome editing.

293 nd conditional mutant mice by coinjection of zygotes with Cas9 mRNA and different guide RNAs (sgRNAs)

294 Co-injection of zygotes with Cas9 mRNA and sgRNA has been proven to be a

295 ovaries, and demonstrated that injection of zygotes with Cas9 mRNA and sgRNA is an efficient and rel

296 itrullination in HEK293TPAD1 cells and mouse zygotes with excellent potency.

297 developmental impact of sperm miRs in early zygotes with single-cell amplification technology, ident

298 efficiency of gene editing in rhesus monkey zygotes, with no detected off-target effects at selected

299 ti locus induced site-directed DNA breaks in zygotes within 6 h of injection, an activity that contin

300 gated within the first cell divisions of the zygote, without being preceded by a common premalignant