ライフサイエンスコーパス: (His)6

1 with the configuration T7 promoter-TIR-regA-His6.

2 n the amino terminal region between Arg5 and His6.

3 d the trapping capacity was ~100 nmol/mg for His6.

4 n reagents phosphine-FLAG and phosphine-FLAG-His6.

5 ce remaining after removal of the C-terminal His6.

6 The 20-kDa His6-Aer2-166 PAS-F1 fragment was purified as an 800-kDa

7 Furthermore, Hyp-1 was expressed with a His6 affinity tag linked to its N terminal region using

8 ty isolation of histidine-tagged Sba1p (Sba1(His6)) after expression in yeast led to coisolation of H

9 Addition of a 6-histidine extension (HisP(His6)) allows a rapid and effective metal affinity purif

10 alysis of the assembly state showed that the His6-alpha and beta subunits released from corresponding

11 The His6-alpha-loop retained significant native structure, a

12 inely yielded approximately 20 mg of soluble His6-alpha-loop/L cell culture.

13 His6 and AHHAHHAAD AHHAHHAAD spiked in a protein digest

14 unction mutations, restoring efficacy to the His6 and d-Phe7 substituted peptides that had lost effic

15 constraint to enhance the beta-turn spanning His6 and D-Phe7, while the pharmacophore group in Arg8 w

16 cytoplasmic domain of MisS were purified as His6 and maltose binding protein fusion proteins, respec

17 NACK120-his6 and NACK100-his6 were dimers in solution.

18 rtant groups, namely, the side chains of the His6 and Phe8 residues.

19 Constructs with C-terminal His6 and V5 epitopes were expressed by transient transfe

20 rthermore, interaction between purified Sba1(His6) and Hsp90 in yeast extracts was inhibited by the b

21 re important for complex formation with Sba1(His6), and residues in both the N-terminal nucleotide bi

22 issociation constant between poly-His, i.e., His6, and the Fe3O4@Al2O3 MNPs was ~10(-5) M and the tra

23 tagged derivative by precipitation with anti-His6 antibodies and by Co2+ affinity chromatography.

24 n7)-Phe8 (3), Sar1-Arg2-Val3-Tyr4-cyclo(Asp5-His6-Apt7)-Phe8 (4), Sar1-Arg2-Val3-Tyr4-cyclo(Glu5-His6

25 t7)-Phe8 (4), Sar1-Arg2-Val3-Tyr4-cyclo(Glu5-His6-Apt7)-Phe8 (5), Sar1-Arg2-Val3-Tyr4-cyclo-(Cys5-His

26 ependent viral infection, as assessed in two His6 artificial receptor systems.

27 ds from the N-terminus of the protein, Arg2, His6, Asn8 and Arg10, interact with the base pairs of th

28 ents carrying nickel-binding histidine tags (his6) at their C termini were also generated.

29 ility shift assays with purified recombinant His6-AUF1 fusion protein.

30 vitro exchange studies reveal that when apo-(His6)-beta2 ((His)beta2) is mixed with beta'2, apo-(His)

31 Preparation of (His6)BglK by nickel-nitrilotriacetic acid-agarose chroma

32 nd cross-reacts with polyclonal antibody to (His6)BglK from K. pneumoniae.

33 on, consistent with the high symmetry of the His6 binding site observed crystallographically.

34 ng an in vitro assembly assay, purified Sba1(His6) bound to Hsp90 only in the presence of adenosine 5

35 in sf-9 cells infected with both p55CDC and His6-BUBR1 recombinant baculoviruses but not in the cell

36 PC components, preferentially associate with His6-BUBR1 resins, but not the control resins.

37 enhances the association between p55CDC and His6-BUBR1.

38 n reagents (phosphine-FLAG or phosphine-FLAG-His6) can be completed in approximately 1 week.

39 ecombinant baculoviruses, sf-9 cells produce His6-Cdc25C antigen with an additional slower mobility b

40 e mapping shows that His6-Prk phosphorylates His6-Cdc25C at two sites in vitro and that the major pho

41 amide gels compared with cells infected with His6-Cdc25C baculovirus alone.

42 rom sf-9 cells co-infected with His6-Prk and His6-Cdc25C baculoviruses, but not with His6-PrkK52R and

43 baculoviruses, but not with His6-PrkK52R and His6-Cdc25C baculoviruses, contains a greatly enhanced k

44 In addition, His6-Cdc25C immunoprecipitated from sf-9 cells co-infect

45 K52R, is capable of strongly phosphorylating His6-Cdc25C in vitro.

46 enhanced kinase activity that phosphorylates His6-Cdc25C in vitro.

47 We have expressed His6-Prk and His6-Cdc25C proteins using the baculoviral vector expres

48 When co-infected with His6-Prk and His6-Cdc25C recombinant baculoviruses, sf-9 cells produc

49 His6-CDPK alpha (1-328), which contained none of the CLD

50 vity of the chimeric enzyme, its addition to His6-CDPK alpha (1-398) resulted in activity that was on

51 s activated only 5-fold, but the activity of His6-CDPK alpha (1-398), which retained nearly half of t

52 (His6-eYFP), and His6-tagged collagenase G (His6-ColG), with sizes ranging from 1 to 11 nm, were use

53 The inactive SpoIVFB.Pro-sigma(K)(1-126)-His6 complex was stable during affinity purification and

54 stent with a 4:2 SpoIVFB.Pro-sigma(K)(1-126)-His6 complex.

55 de with 20-residue-long, thrombin-cleavable, His6-containing N-terminal extensions.

56 pha-MSH, gamma-MSH, and Ac-Nle4-cyclic-[Asp5,His6,D-Phe7,Arg8,Trp9,Lys10]NH2 (MT-II).

57 peptides derived from MTII, Ac-Nle-cyclo(Asp-His6-D-Phe7-Arg8-Trp-Lys)-NH2 (a pan-agonist at the mela

58 (NDP-MSH), by first defining the role of the His6-d-Phe7-Arg8-Trp9 residues in receptor activation (E

59 orbent assay (ELISA) using affinity-purified his6-DEK fusion protein.

60 ein fusion with Plasmodium knowlesi PSD (MBP-His6-Delta34PkPSD) as the enzyme.

61 the His6 epitopes did not appear to mediate His6-dependent viral infection, as assessed in two His6

62 ny genes whose expression requires DnaA, and His6-DnaA binds to the promoters of gcrA, ftsZ and podJ

63 ovel cyclic SHU9119 analogues (Ac-Nle4-[Asp5-His6-DNal(2')7-Arg8-Trp9-Lys10]-NH2) modified in positio

64 ng in a carboxyl-terminal hemagglutinin (HA)-His6 epitope.

65 The His6 epitopes did not affect the native infectivity of A

66 In addition, the His6 epitopes did not appear to mediate His6-dependent v

67 This suggested the His6 epitopes in HVR2 and HVR5 were exposed on virion su

68 yme-linked immunosorbent assay and found the His6 epitopes incorporated in HVR2 and HVR5 could bind t

69 The His6 epitopes were expressed in all modified hexon prote

70 either hemagglutinin (HA) or hexahistidine (His6) epitopes at the C-terminus.

71 in vitro assays, the degradation of purified His6-Epsilon by the His6-LonBs, ClpPBs, and ClpXBs prote

72 Small molecular mass molecules (His6) exhibit a high binding ability to all PNTs polymer

73 -tagged enhanced yellow fluorescent protein (His6-eYFP), and His6-tagged collagenase G (His6-ColG), w

74 Full-length wild-type FadR and His6-FadR bound oleoyl-CoA and myristoyl-CoA with simila

75 Complexes purified from brains of transgenic His6-FLAG-YFP-NL2 mice showed enrichment in the Gene Ont

76 We find the His6-FLAG-YFP-NL2 to be a suitable tag, with the unampli

77 The PcrA protein was overexpressed with a His6 fusion at its amino-terminal end.

78 BIG2, synthesized as a His6 fusion protein in Sf9 cells, accelerated guanosine

79 iserum raised against an overexpressed MrpG'-His6 fusion protein showed that MrpG was present in the

80 The BFP assembly complex, containing a BfpB-His6 fusion protein, was chemically cross-linked in situ

81 protein microarrays constructed with double-His6 GFP demonstrated greater detection sensitivity with

82 ution, while the dissociation rate of double-His6 GFP from Ni-NTA chips in SPR (BIAcore) was 10 times

83 en used to isolate His6-T7 bacteriophage and His6-GroEL directly from cell lysates.

84 formed cryo-electron microscopy (cryo-EM) of His6-GroEL obtained from clarified E. coli lysates.

85 activity in cytosolic extracts, and purified His6-GT78 exhibited alpha3GalT-activity toward a synthet

86 applied for detection of antibodies against His6-H5 HA in serum of vaccinated hen using serial 10-fo

87 of the recombinant His-tagged hemagglutinin (His6-H5 HA), (v) filling free space with BSA.

88 Purified HisP(his6) has a strong tendency to precipitate; 5 mM ATP and

89 HisP(his6) has been characterized with respect to substrate a

90 tracts of a McArdle cell line overexpressing His6-hemagglutinin-tagged, rat APOBEC-1 using metal-chel

91 His tag (His6), His-tagged enhanced yellow fluorescent protein (H

92 hift movements of the 2H NMR signals for the His6, His13, and His14 side chains.

93 ing of L-(-)-nicotine to histidine residues (His6, His13, and His14) in the peptide.

94 A model is proposed in which Cu(2+)-His6/His13 and Cu(2+)-His6/His14 sites alternate along t

95 oposed in which Cu(2+)-His6/His13 and Cu(2+)-His6/His14 sites alternate along the fibril axis on oppo

96 N-terminal-containing His6-tagged insulysin (His6-IDE) with proteinase K led to the initial cleavage

97 ather than the sigmoidal curve obtained with His6-IDE.

98 The protonation/deprotonation equilibrium of His6 influences the reduction potential of the protein i

99 nucleotides encoding six histidine residues (His6) into different HVRs in the Ad5 genome.

100 HisP(his6) is active as a dimer, binds ATP with a Kd value of

101 HisP(his6) is indistinguishable from underivatized HisP when

102 In contrast to the intact complex, HisP(his6) is not inhibited by vanadate but is inhibited by N

103 nding half-site capable of interacting with (His6)jRXR fusion protein was identified in the promoters

104 Finally, an antibody prepared against (His6)jRXR showed that full-length jRXR is expressed at a

105 -binding (Asp347) residue were essential for His6-LAP-A activity, as most R431 and D347 mutant His6-L

106 The E429D His6-LAP-A enzyme had Km and kcat values similar to the

107 Glu334, Arg336, and Lys262, was evaluated in His6-LAP-A fusion proteins expressed in Escherichia coli

108 Sixty-five percent of the mutant His6-LAP-A proteins were unstable or had complete or par

109 The R431K His6-LAP-A that retained the positive charge had partial

110 Km and kcat values similar to the wild-type His6-LAP-A.

111 LAP-A activity, as most R431 and D347 mutant His6-LAP-As did not hydrolyze dipeptides.

112 The activity of hexameric His6-LAP-As on Xaa-Leu and Leu-Xaa dipeptides was tested

113 of Lys354 (a catalytic residue) resulted in His6-LAP-As that cleaved dipeptides at slower rates.

114 reover, LdSec13 specifically interacted with His6-LdSec31(1-603), and LdSec31 bound the prebudding co

115 A recombinant His6-LipL41 fusion protein was expressed in Escherichia

116 degradation of purified His6-Epsilon by the His6-LonBs, ClpPBs, and ClpXBs proteases from B. subtili

117 d to estimate the secondary structure of the His6 loop, revealing an ordered folding composed of 23%

118 rther show that purified A. pleuropneumoniae His6-Lrp binds in vitro to the A. pleuropneumoniae promo

119 The strategy utilizes a dual His6-maltose binding protein (HisMBP) affinity tag that

120 uberculosis (MtUGM) was obtained from a dual His6-MBP-tagged MtUGM construct.

121 Purified His6-MM1854 occurred as a homodimer of 29-kDa subunits,

122 ng sites: the His3Asp motif (site 1) and the His6 motif (site 2).

123 titrations support the formation of a Zn(II)-His6 motif.

124 t7)-Phe8 (6), Sar1-Arg2-Val3-Tyr4-cyclo(Cys5-His6-Mpc7)-Phe8 (7), Sar1-Arg2-Val3-Tyr4-cyclo(Hcy5-His6

125 Phe8 (8), and Sar1-Arg2-Val3-Tyr4-cyclo(Hcy5-His6-Mpc7)-Phe8 (9), where Apt stands for 4-amino-trans-

126 7)-Phe8 (5), Sar1-Arg2-Val3-Tyr4-cyclo-(Cys5-His6-Mpt7)-Phe8 (6), Sar1-Arg2-Val3-Tyr4-cyclo(Cys5-His6

127 c7)-Phe8 (7), Sar1-Arg2-Val3-Tyr4-cyclo(Hcy5-His6-Mpt7)-Phe8 (8), and Sar1-Arg2-Val3-Tyr4-cyclo(Hcy5-

128 oresis mobility shift analysis revealed that His6-NasR bound specifically to nasF leader RNA.

129 t the terminator site, and that MBP-NasR and His6-NasR proteins both caused transcription readthrough

130 sion form (MBP-NasR) and a His6-tagged form (His6-NasR).

131 Incorporation of His6-OPH in nano-OPH preserves catalytic activity and in

132 ses the immune and inflammatory responses to His6-OPH in vivo as determined by anti-OPH IgG and cytok

133 ic coupling of the hexahistidine tagged OPH (His6-OPH) and poly(ethylene glycol)-b-poly(l-glutamic ac

134 Removal of this tag from His6-p38 MAPKalpha, prior to its activation, is essentia

135 We found that addition of recombinant His6-p9 to egg extracts results in a pronounced delay of

136 ic and inhibition studies were performed for His6-PDK1(deltaPH)-catalyzed phosphorylation of PDK1-Tid

137 analogs were Sar1-Arg2-Val3-Tyr4-cyclo(Cys5-His6-Pen7)-Phe8 (3), Sar1-Arg2-Val3-Tyr4-cyclo(Asp5-His6

138 The double-His6 peptide has the potential to be a universal tag for

139 We inserted a His6 peptide into an ecotropic envelope protein (Env) by

140 region sequence with a sequence encoding the His6 peptide.

141 Plk3, but not by the kinase-defective mutant His6-Plk3(K52R), GST-p53 was recognized by an antibody s

142 recipitated from sf-9 cells co-infected with His6-Prk and His6-Cdc25C baculoviruses, but not with His

143 We have expressed His6-Prk and His6-Cdc25C proteins using the baculoviral

144 When co-infected with His6-Prk and His6-Cdc25C recombinant baculoviruses, sf-9

145 Further studies reveal that His6-Prk phosphorylates Cdc25C on serine216, a residue a

146 Moreover, phosphopeptide mapping shows that His6-Prk phosphorylates His6-Cdc25C at two sites in vitr

147 Purified recombinant His6-Prk, but not a kinase-defective mutant His6-PrkK52R

148 and His6-Cdc25C baculoviruses, but not with His6-PrkK52R and His6-Cdc25C baculoviruses, contains a g

149 His6-Prk, but not a kinase-defective mutant His6-PrkK52R, is capable of strongly phosphorylating His

150 angiotensin II (Asp1-Arg2-Val3-Tyr4-Val/IIe5-His6-Pro7-Phe8, AII), the synthesis and biological testi

151 T7 promoter on plasmid pSA1 yielded a RegA69-His6 protein that binds nickel-Sepharose and elutes with

152 bout by the structural change resulting from His6 protonation.

153 The resulting His6-PubC fusion proteins were purified by Ni-NTA affini

154 stidine6-tagged receptor-associated protein (His6-RAP) with high affinity, although variants lacking

155 siRNA to C33-A cells using oligofectamine or His6 reducible polymers, siRNA uptake was quantified by

156 gene products in which C-terminal tags (FLAG-His6) replace the native glycosylphosphatidylinositol an

157 a consequence of protonation of the surface His6 residue, is identified.

158 of Ad type 2 E3-19K tagged with a C-terminal His6 sequence in baculovirus-infected insect cells.

159 The His6 sequence is used as a ligand for the one-step affin

160 at their amino terminus, the peptide Met Gly His6 Ser Gly Leu Phe Lys Arg/, where Leu Phe Lys Arg/ is

161 opose that removing the interaction with the His6 side chain reorients the peptide within the binding

162 ude and coincides with Mn(II) binding at the His6 site as well as other sites.

163 The Zn(II)-His6 site of CP expands the known biological coordinatio

164 finity and specific binding of Mn(II) to the His6 site of human calprotectin.

165 ution, affording an unprecedented biological His6 site.

166 o SPR assay formats, with either mAb10F12 or His6-SNAP-25 coupled to the biosensor chip.

167 RegA69-His6 synthesized in E. coli S30 or wheat germ extracts d

168 fouling properties were then used to isolate His6-T7 bacteriophage and His6-GroEL directly from cell

169 s formed between 1-Cys Prx with a C-terminal His6 tag and GST pi upon incubation of the two proteins

170 engineered into an expression vector with a His6 tag at its amino terminus, and the protein was expr

171 ombinant alpha-galactosidase molecule with a His6 tag at its C-terminus was constructed by an overlap

172 The synthetic technique makes use of a His6 tag at the C terminus of the target polypeptide cha

173 1) was expressed using a vector containing a His6 tag at the N terminus.

174 Moreover, the double-His6 tag could serve simultaneously both for protein imm

175 The presence of a His6 tag enables the isolation of peptide or protein pro

176 ted into the pRSET vector and expressed as a His6 tag fusion protein in Escherichia coli.

177 de, the M. xanthus gene was expressed with a His6 tag in E. coli cells.

178 ary of mutants whose combined members have a His6 tag inserted at likely every site in the original p

179 Display of the His6 tag on the surface of Env endowed the vectors with

180 modified surfaces than a conventional single-His6 tag or two single-His6 tags at N- and C-termini.

181 attached to the carboxy end of an N-terminal His6 tag that binds to nickel (Ni2+).

182 Therefore, addition of the His6 tag to the C-terminus of RB69 RegA does not dramati

183 A His6 tag was cloned at the N terminus, along with R403Q,

184 Under denaturing conditions when the His6 tag was used, 84% of samples were purified.

185 NBD1-R (amino acids 404-830, in fusion with His6 tag) were expressed as single proteins in Escherich

186 gene, encoding a protein with an N-terminal His6 tag, was expressed in Escherichia coli.

187 9-kDa chimeric proteins, having a C-terminal His6 tag, were secreted by Saccharomyces cerevisiae, usi

188 The results show that His6 tag-assisted chemical protein synthesis is a useful

189 ion vector that also introduced a C-terminal His6 tag.

190 que except that the MutL protein carried the His6 tag.

191 60% for the Nanobody lacking the C-terminal His6 tag.

192 GFP), stable and tight binding of the double-His6 tag/Ni-NTA interaction was demonstrated by competit

193 Here we describe a double-hexahistidine (His6) tag sequence, comprising two hexahistidines separa

194 1 KCS and its mutants were engineered with a His6-tag at their N-terminus, and expressed in Saccharom

195 on in conjunction with immobilized metal ion/His6-tag interactions to prepare highly purified recombi

196 Overexpression of GT78 with a His6-tag resulted in a 120-fold increase in GalT-activit

197 ptors and an NTA moiety to interact with the His6-tag upon cocomplexation of Ni(2+) ions.

198 terminus with either a FLAG-epitope tag or a His6-tag.

199 in Escherichia coli as catalytically active His6 tagged proteins, purified to homogeneity by affinit

200 We purified polyhistidine (His6)-tagged and native Escherichia coli MiaA tRNA preny

201 The purified hexahistidine (His6)-tagged Pseudomonas aeruginosa RhlI protein was sub

202 To test this hypothesis, a six-histidine (His6)-tagged version of MM1854 was produced.

203 and detected in immunoblots using N-terminal His6-tagged AgrD.

204 In addition, GST-NR2B and His6-tagged alpha-actinin can bind simultaneously to mon

205 mbinant sorcin bound to cardiac and skeletal HIS6-tagged alpha1 C termini immobilized on Ni2+ resin.

206 ies assembled in vivo in the presence of the His6-tagged and untagged Rpb3.

207 Purified recombinant His6-tagged apobec-1 immobilized on beads depleted >90%

208 ypothesis, R. sphaeroides mutants expressing His6-tagged bc1 complexes with mutations at three aromat

209 yellow fluorescent protein (His6-eYFP), and His6-tagged collagenase G (His6-ColG), with sizes rangin

210 Of the his6-tagged constructs derived from NACK, NACK100 was in

211 Similarly, His6-tagged densin-180 or alpha-actinin fragments associ

212 latory protein was purified as an N-terminal His6-tagged derivative and characterised both with and w

213 In the present study, His6-tagged E1 alpha2 beta2 tetramers (171 kDa) denature

214 binding protein fusion form (MBP-NasR) and a His6-tagged form (His6-NasR).

215 nterfacial properties of a carboxyl-terminal His6-tagged form of apoB20.1 (apoB20.1H).

216 spectrometry confirmed production of intact, His6-tagged forms of CYP144A1-FLV and -TRV, with EPR dem

217 We have expressed His6-tagged fragments containing the sequence of the C-t

218 6E mutants of peripherin-2 were expressed as His6-tagged full-length proteins in Madin-Darby canine k

219 atable peptide sequence to the C-terminus of His6-tagged green fluorescent protein (GFP).

220 Treatment of an N-terminal-containing His6-tagged insulysin (His6-IDE) with proteinase K led t

221 The protocol described here uses recombinant His6-tagged KDPG aldolase for the synthesis of (S)-4-hyd

222 His6-tagged M. thermautotrophicus type II IPP isomerase

223 Gene-targeted mice that contain a His6-tagged mouse c-Myc cDNA, Myc(His), inserted head to

224 We demonstrated that the His6-tagged MuLV could be produced to high titers and co

225 HPLC assay analysis of each His6-tagged mutant indicated that F337I successfully pro

226 A His6-tagged NR2B fragment associates with GST-densin or

227 In this work, the His6-tagged PduS cobalamin reductase from S. enterica wa

228 Expression of a human His6-tagged PKC-theta in Jurkat cells and purification b

229 d-biotin-tris-NTA (BTN) hybrid compound, any His6-tagged protein can be immobilized on the surface of

230 proteins in the same cell and isolating the His6-tagged protein followed by MutS immunoblotting afte

231 RNA ligands were selected by binding to His6-tagged proteins and chromatography on nickel(II) ni

232 We also characterized His6-tagged proteins enriched on-plate by the Fe3O4@Al2O

233 he pET15b constructs produced NH2-terminally His6-tagged proteins.

234 BIAcore) was 10 times slower than for single-His6-tagged proteins.

235 formation by wild-type and mutant XRCC3 with His6-tagged Rad51C upon co-expression in bacteria, nicke

236 n investigated by Raman spectroscopy using a His6-tagged recombinant protein that self-assembles in v

237 These proteins have been expressed as Met-His6-tagged recombinant proteins and purified by metal c

238 The His6-tagged Sec7 domain from ySec7p (rySec7d) synthesize

239 for the assembly of growth factors employing His6-tagged single-domain antibodies (VHH).

240 ach can be used to determine quickly whether His6-tagged species are present in a sample.

241 We capture His6-tagged TFs using nickel-nitrilotriacetic acid (Ni-N

242 His6-tagged Tsc10p was expressed in Escherichia coli and

243 ecifically recognizing Rpl17 or Rpl39, and a His6-tagged version of Rpl4, we established that all thr

244 A His6-tagged version of the SCO1135 protein product was s

245 A recombinant His6-tagged version of this protein was subsequently pro

246 e-steady-state studies were performed on the His6-tagged wild-type (WT) enzyme, several active site m

247 Dimerization was assayed by expressing His6-tagged wild-type and non-tagged deletion mutant pro

248 essed in Escherichia coli, and the resulting His6-tagged WreQ protein was purified.

249 that encode the PAS domain with and without His6 tags and expressed the PAS peptides in E. coli.

250 a conventional single-His6 tag or two single-His6 tags at N- and C-termini.

251 xpressed in Escherichia coli with N-terminal His6 tags to facilitate purification.

252 quipped lipid to bind the proteins via their His6-tags to the lipid membrane were used to bind two di

253 This deletion reduced multimerization of His6-tailed KorB protein in vitro and greatly reduced bi

254 chia coli as recombinant proteins containing His6 tails.

255 as equipped with a C-terminal hexahistidine (His6) tether to facilitate the assembly on betaCD surfac

256 His6-Thg1p purified from Escherichia coli catalyzes the

257 otein with one of eight tags: hexahistidine (His6), thioredoxin (Trx), small ubiquitin-related modifi

258 Conversely, His6 to Ala6 substitution reduced receptor activation bu

259 C-terminal end with an epitope (FLAG) and a His6 tract.

260 e wt TrfA which is largely dimeric, purified His6-TrfA(G254D/S267L) is primarily monomeric.

261 His6-TrfA(P151S) and His6-TrfA(S257F) purify as dimers,

262 His6-TrfA(P151S) and His6-TrfA(S257F) purify as dimers, and when expressed in

263 s led to the identification of several VSV-G-His6 variants that were able to package high-titer viral

264 However, MBP-NACE-his6 was completely resistant to cleavage by factor Xa,

265 The limit of detection for His6 was, therefore, as low as ~400 amol.

266 se-insensitive protein variant (FixK2(C183S)-His6) was used in which Cys-183 was replaced with serine

267 The apparent Km values of PduS-His6 were 10.1+/-0.7 muM for NADH and 67.5+/-8.2 muM for

268 NACK120-his6 and NACK100-his6 were dimers in solution.

269 ng recombinant Shiga-like toxins tagged with His6 were used as the samples to further demonstrate tha

270 roximately 100-fold more active than Arg6 or His6, whereas Glu6 and (SerSerGly)2 were inactive.