ライフサイエンスコーパス: -attached

1 enoic acids and keto-eicosatetraenoic acids--attached to either phosphatidylethanolamine or phosphati

2 hat generates force by cycling between actin-attached (strong binding: ADP or rigor) and actin-detach

3 n of these with the contributions from actin-attached myosin heads determines the behavior of these r

4 n contrast to the detached partners of actin-attached heads.

5 Here the number of actin-attached motors per half-myosin filament (n) during V0 s

6 Here, the number of actin-attached motors per half-thick filament during V0 shorte

7 ht in some way to swing axially on the actin-attached motor domain, thus propelling the actin filamen

8 ilt of the lever arm domain during the actin-attached working stroke.

9 ng between the two lobes of the RLC to actin-attached myosin heads in muscle fibers, and suggest that

10 The Ala-attached support has been used to synthesize tri- to oct

11 latory process in which a subset of alveolus-attached AMs intercommunicates immunosuppressive signals

12 movement displacements recorded from animal-attached electronic tags to show that diverse marine pre

13 The auxiliary-attached cyclobutanecarboxamides 15a, 15g, and 15h, prep

14 Baboon-attached motion/GPS tracking collars showed that raiding

15 enzyme still contained normal levels of BclA-attached oligosaccharides.

16 th of a 2D view into the amount of a 3D bone-attached root surface area (BA-RSA) by using a dental la

17 Osteomyelitis pathogens are bone-attached biofilms, making antibiotic delivery challengin

18 e animals, its occurrence in sessile (bottom-attached) species is limited to members of the ancient p

19 ding strain 14DCB1, the most positive carbon-attached H atom discriminates active from nonactive chlo

20 Fragmentation of lithium cation-attached alpha- and beta-O-methyl-glucopyranoside precur

21 Cell-attached and whole-cell recordings revealed that excitat

22 Cell-attached capacitance measurements indicated that inhibit

23 Cell-attached patch clamp recordings confirmed that perisomat

24 Cell-attached patch recording on the hair-cell apical membran

25 Cell-attached patch recordings from the soma of CA1 neurons w

26 Cell-attached patch recordings of unitary LTCC currents confi

27 Cell-attached patch recordings were performed in transfected

28 Cell-attached patch-clamp analysis of cultured astrocytes rev

29 Cell-attached patches and whole-cell currents from this mutan

30 Cell-attached recording is extensively used to study the firi

31 Cell-attached recordings reveal a highly reliable and precise

32 However, fewer than 1 in 100 cell-attached patches from spinal neurones contained glycine

33 ng experiments show that establishing a cell-attached recording can result in an increased somatic Ca

34 uorescence microscopy, amperometry, and cell-attached capacitance measurements.

35 urthermore, concurrent Ca2+ imaging and cell-attached electrophysiological recordings demonstrated th

36 ic nucleus (SCN), using patch clamp and cell-attached electrophysiological recordings, RT-PCR, immuno

37 unitary currents in the inside-out and cell-attached modes of the patch-clamp technique.

38 terized by carbon fiber amperometry and cell-attached patch capacitance measurements.

39 tigated by carbon fiber amperometry and cell-attached patch capacitance measurements.

40 ta-cell were examined by whole-cell and cell-attached patch clamp capacitance measurements in INS 832

41 (GFP) tag and performed whole-cell and cell-attached patch clamp recordings.

42 ents were observed using whole-cell and cell-attached patch configurations.

43 sted in voltage-clamped whole cells and cell-attached patches by bath flow and patch pressure, respec

44 Nucleated and cell-attached patches revealed that VGSC density at the soma

45 nd GABA for whole-cell, perforated, and cell-attached recording in 240 neurons.

46 Whole-cell current-clamp and cell-attached recordings from labeled RMTg neurons were perfo

47 Using simultaneous calcium imaging and cell-attached recordings from three genetically labeled DSGC

48 al-590, combined two-photon imaging and cell-attached recordings revealed that, despite the relativel

49 ision of firing in perforated-patch and cell-attached recordings.

50 Cx43 hemichannels were detected in both cell-attached and inside-out patch recordings in C6 cells exp

51 8 CFTR channel activity evident in both cell-attached membrane patches and planar phospholipid bilaye

52 erties of A52S recombinant receptors by cell-attached patch-clamp recording of single-channel current

53 In this study, by cell-attached recordings in the developing optic tectum of ze

54 Single-channel cell-attached analysis demonstrated that PS primarily affecte

55 Single-channel cell-attached patch clamp showed comparable unitary conductan

56 recordings in all four configurations (cell-attached, inside-out, outside-out, and whole-cell).

57 conductance microscopy and conventional cell-attached patch clamp with a software modification that a

58 Finally, cell-attached recordings indicated that GABA(A) receptor acti

59 ent protein (RFP) and targeted them for cell-attached electrophysiological recordings.

60 yocytes) and sealed to the membrane for cell-attached recording of ion channels.

61 of recording single GABA channels from cell-attached and outside-out patches and also introduced som

62 we recorded steady-state currents from cell-attached and outside-out patches.

63 channel activity could be recorded from cell-attached patches of acutely dissociated neurons bathed i

64 Single-channel recordings from cell-attached patches revealed that basal and evoked IK(ATP)

65 ) single-channel openings recorded from cell-attached patches, an effect that was blocked by the PKG-

66 In the majority of recordings from cell-attached patches, MS channels have a conductance of appr

67 e recorded single-channel currents from cell-attached patches.

68 Here, we used a classifier derived from cell-attached recordings to separate putative pyramidal cells

69 this, we used in vivo two-photon guided cell-attached recording in conjunction with spatially restric

70 e established two-photon imaging guided cell-attached recordings from genetically labeled inhibitory

71 Here, cell-attached capacitance recording was used to study single

72 Here, cell-attached recordings in rat primary auditory cortex revea

73 These Cl(-) channels were observed in cell-attached apical patches of principal cells after stimula

74 f membrane excitability, we recorded in cell-attached configuration spontaneous action currents from

75 find that this rate depends on time in cell-attached mode, on pipette potential, and on pipette ioni

76 ure of this activation was confirmed in cell-attached patch clamp experiments with trypsin added to t

77 and microvesicle fusion was enhanced in cell-attached patches and dense-core vesicle fusion pores had

78 ibited currents of similar magnitude in cell-attached patches as in inside-out patches exposed to 10

79 bath solution easily activated TRPA1 in cell-attached patches but failed to do so in inside-out or ou

80 otoxin- or PEG-CAT-sensitive fashion in cell-attached patches but had little effect on channel openin

81 In cell-attached patches from cardiac myocytes and Na(V)1.5-expr

82 In cell-attached patches from hippocampal neurones from the Tg25

83 In cell-attached patches from ventral horn neurones, 1 mM glycin

84 e activity of single K(ATP) channels in cell-attached patches of granule cells in the mouse dentate g

85 In cell-attached patches of mesenteric arterial smooth muscle ce

86 t recombinant GluN1/GluN2B receptors in cell-attached patches of transiently transfected HEK293 cells

87 we now investigate TRPM8 inhibition in cell-attached patches using HEK293 cells expressing TRPM8 alo

88 for activation of the 20 pS channel in cell-attached patches was approximately 200 nm.

89 In cell-attached patches with 140 mM KCl and 1 mM Mg2+ in the ba

90 ere voltage-dependent, highly active in cell-attached patches, activity decreasing markedly on hyperp

91 At pH 7.9 in cell-attached patches, ifenprodil increased the occupancy of

92 In cell-attached patches, number of open channels x open probabi

93 s and mechanosensitivity was studied in cell-attached patches.

94 gly, increased cytoskeletal rigidity in cell-attached patches.

95 e rise to very small inward currents in cell-attached patches.

96 gATP depletion, and channel activity in cell-attached patches.

97 118 pS and 185 pS were also observed in cell-attached recordings from these three groups.

98 In cell-attached recordings of Kir6.1/SUR2B single channel activ

99 In cell-attached recordings of spontaneous pacemaking, 10 mM 4-A

100 In cell-attached recordings, mitral cells responded to high freq

101 In cell-attached single-channel recordings, where 3alpha5alphaP

102 Here, we employed minimally invasive cell-attached patch-clamping, single-cell qPCR and specific s

103 ivated ion channels in arterial myocyte cell-attached patches that were inhibited by TMEM16A antibodi

104 tion locally to the external surface of cell-attached patches through the recording pipette.

105 e determined by a race between entry of cell-attached virions and competing inactivation processes, w

106 experimental conditions are optimized, cell-attached recordings in small neurons may report erroneou

107 Here we perform cell-attached recording of molecular layer interneurons in ce

108 By performing cell-attached current and capacitance recordings predominantl

109 CR-induced TRPC3c channel opening rate (cell-attached patch) matched the maximum activation achieved

110 -seal whole-cell somatic and loose-seal cell-attached axonal/dendritic patch-clamp recordings and com

111 Simultaneous cell-attached loose-patch recordings of Na(+) currents on eac

112 In brain slices, cell-attached recordings of membrane potential show that CO2/

113 archical cellular structure by stacking cell-attached microplate structures with specific configurati

114 We used in vivo two-photon targeted cell-attached recording to compare the response properties of

115 gurations of the patch-clamp technique (cell-attached, inside-out, whole-cell, outside-out and perfor

116 electrophysiological recordings in the cell-attached configuration demonstrated that activated alpha

117 In the cell-attached configuration, H(2)O(2) significantly increased

118 f the outer and inner monolayers in the cell-attached configuration, whereas a substantial difference

119 This implies that the magnitude of the cell-attached current is regulated not only by intracellular

120 es host extracellular matrix and in the cell-attached form participates in bacterial adherence.

121 rrent amplitude to that measured in the cell-attached mode.

122 suitable for kinetic analysis were the cell-attached ones.

123 ifunctional surface protein that in the cell-attached or secreted form cleaves host extracellular mat

124 We find that of the cell-attached particles, between 20 and 50% ultimately penetr

125 ted nucleus accumbens neurons using the cell-attached patch clamp configuration.

126 Using the cell-attached patch clamp technique, we investigated the hypo

127 rom single TRPV1 ion channels using the cell-attached patch clamp technique.

128 s for recording LTCC currents using the cell-attached patch-clamp technique.

129 6 activations were only observed in the cell-attached patches but not in the inside-out patches.

130 Unexpectedly, these cell-attached currents are significantly smaller (by approxim

131 Application of negative pressure to cell-attached patches (-20 mmHg) caused a 19-fold increase in

132 fluorometry is superior to traditional cell-attached MA for measuring the intrinsic mechanical prope

133 Here, we used cell-attached and whole-cell recordings in vitro to study act

134 to be kiss-and-run, as determined using cell-attached capacitance recordings in endocrine cells, pitu

135 Direct measurement of I(KA) using cell-attached patch-clamp recordings revealed that there was

136 By using cell-attached patch-clamp recordings, we found that these neu

137 +)-free/high-Mg(2+) bath solution using cell-attached recording.

138 selected independent of spiking, using cell-attached recordings and two-photon calcium imaging, in t

139 Using cell-attached recordings in vivo, we demonstrate that electri

140 Here we use in vivo cell-attached and whole-cell voltage-clamp recordings to reve

141 In vivo cell-attached recordings revealed differences between the sou

142 Visualized in vivo cell-attached recordings show that division by PV neurons alt

143 vesicles in mouse chromaffin cells with cell-attached capacitance measurements that offer millisecond

144 r membrane neck (the fission-pore) with cell-attached capacitance measurements to detect CME of singl

145 With cell-attached recording bath application of 10 nm ET-1 evoked

146 educed ATP-sensitivity display non-zero cell-attached currents.

147 We show that the stability of chromosome-attached kinetochore-MTs (K-MTs) is important for bipola

148 and 11-times higher than those of covalently-attached GOx (CA) on acid-treated CNTs and simply-adsorb

149 on between the membrane and the cytoskeleton-attached protein anchors embedded in the membrane matrix

150 Erk signaling also regulates PDH flux in ECM-attached cells.

151 -overexpressing cells when compared with ECM-attached cells.

152 egatively-charged AuNC@BSA electrostatically-attached onto MSN-NH3(+), affording highly loaded pH-res

153 End-attached filaments transiently paused but resumed growth

154 deformation of a monolayer of chemically end-attached cartilage aggrecan on a microcontact printed su

155 Aggrecan was chemically end-attached onto gold-coated planar silicon substrates and

156 These end-attached PNA amphiphiles (PNAA) partition to nonionic mi

157 ing cellular uptake studies with fluorescein-attached LPA4 (F-LPA4) alone or with the mixture of LPA4

158 V pressures are precisely matched in the GUV-attached configuration, breaking the patch membrane also

159 first time, that the size of the heteroatom-attached alkyl group affects the rate of conjugate addit

160 In hindlimb-attached semi-isolated preparations, passive flexion-ext

161 rization and depolymerization at kinetochore-attached microtubule ends.

162 ce and also by destabilizing the kinetochore-attached microtubule tip.

163 These kinetochore-attached K-fibres generate pushing and pulling forces, w

164 rinsic-switching time of the two kinetochore-attached microtubule fibres, with the centromeric spring

165 (cFLIP) and TNFalpha stimulation of laminin-attached cells in which cFLIP had been downregulated res

166 he first total synthesis of the amino linker-attached, conjugation-ready tetrasaccharide of O-PS of Y

167 ctivity relationships of bivalent and linker-attached compounds in mAChRs.

168 reactivity-based one-pot assembly of linker-attached Lc(4) and IV(2)Fuc-Lc(4), which are components

169 , FHL-1, CFHR1, and plasminogen, and the Lpd-attached regulators are relevant for innate immune escap

170 ues when they were incubated with lymphocyte-attached HIV-1.

171 re PI3K/mTOR signalling is decreased, matrix-attached human mammary epithelial cells upregulate and i

172 responses to PI3K/mTOR inhibition in matrix-attached cells resemble well-conserved stress responses

173 of mRNA fluctuations among individual matrix-attached epithelial cells, we found that a subset of FOX

174 apoptosis of matrix-detached, but not matrix-attached cancer cells, suggesting that matrix-attached c

175 We stochastically profiled matrix-attached human epithelial cells in a three-dimensional c

176 ttached cancer cells, suggesting that matrix-attached cells use alternate mechanisms to maintain nutr

177 inner matrix-deprived cells, whereas matrix-attached cells are resistant.

178 pinning, and filament bending of a membrane-attached cytoskeletal filament can be sufficient to pres

179 suggests that Twinkle is part of a membrane-attached replication platform.

180 f snarls and accumulation of excess membrane-attached bundles.

181 We report that the extracellular membrane-attached protein nyctalopin is required for the normal e

182 and that ZipA-stabilized FtsZ forms membrane-attached spiral-like structures in the bacterial cytopla

183 relative disposition of their inner membrane-attached export platform, C-ring/pods and ATPase complex

184 cally analogous to an intracellular membrane-attached contractile phage tail.

185 rate that hSnf7 proteins form novel membrane-attached filaments that can promote or stabilize negativ

186 lized protein machinery composed of membrane-attached (t-SNARE) and vesicle-attached (v-SNARE) protei

187 , and on the thermal fluctuation of membrane-attached beads.

188 In simulations of membrane-attached protein complexes, interactions between amino a

189 s evolutionarily conserved class of membrane-attached proteins regulates key aspects of APP-dependent

190 Our data indicate that membrane-attached vesicles comprise the readily releasable pool o

191 In green sulfur bacteria, the membrane-attached Fenna-Matthews-Olson (FMO) antenna protein func

192 ently labeled CaM or CaBP1 with the membrane-attached proximal C terminus (pCT) and the N terminus (N

193 somes, it is twice as stiff, due to membrane-attached protein components.

194 The transition from soluble to membrane-attached ribosomes occurs shortly after the first transm

195 porting checkpoint proteins from microtubule-attached kinetochores to spindle poles.

196 mediated removal of Spindly from microtubule-attached kinetochores, rather than poleward transport pe

197 methods, we solved structures of microtubule-attached, dimeric kinesin bound to an ATP analog.

198 ly motif mutants are retained on microtubule-attached kinetochores along with checkpoint proteins, re

199 cleotide states encompassing the microtubule-attached kinetic cycle of a kinesin motor.

200 on between Bir1p and Sli15p resulted in mono-attached kinetochores, suggesting that the Bir1p-Sli15p

201 Incubation of human tissue with monocyte-attached HIV resulted in productive tissue infection.

202 n, with greater SWV values within the muscle-attached region than those outside of the muscle-attache

203 ched region than those outside of the muscle-attached region.

204 sociation with NEMO leads to removal of NEMO-attached linear polyubiquitin chains and subsequent inhi

205 These ECS cells grow as non-attached spheroids, and display enhanced migration and i

206 tric epithelium and a larger subgroup of non-attached bacteria that are freely swimming.

207 K) creates a permanent flap that remains non-attached to the underlying laser-modified stroma.

208 Many NP-attached ligands, however, are difficult to ionize by LD

209 and uses it as a substratum to deliver ParB-attached cargo DNA, and ParB drives ParA dynamics, allow

210 ysiological characteristics between particle-attached and free-living MGIIs.

211 of living organisms and settling of particle-attached organisms lead to uneven distributions of biota

212 that primary successions may shape particle-attached bacterial communities in the ocean and that rap

213 rine particles and demonstrate that particle-attached communities undergo rapid, reproducible success

214 Like ZA itself, the PGN-attached drug (PGN-ZA) binds specifically to viral neura

215 iferative effect on cells growing as plastic-attached monolayer cultures (i.e. standard tissue cultur

216 ng causes shortening and bending of platelet-attached fibers, resulting in formation of fiber kinks.

217 olymer monomer with ssDNA probe sequence pre-attached.

218 holes to form fiber networks that were "pre-attached" to the substrate and other fibers.

219 its heparin ligand, as induced by a protein-attached lanthanide spin label, provided structural rest

220 ns are introduced nonuniformly along protein-attached HS polysaccharide chains by specific enzymes.

221 The detached retina was successfully re-attached surgically and the subretinal fluid was gradual

222 o, but the effects of the number of receptor-attached phosphates on this interaction remain controver

223 Thus, the contribution of receptor-attached phosphates to arrestin binding varies depending

224 We found that a single receptor-attached phosphate does not facilitate arrestin binding,

225 favorable for its interaction with receptor-attached phosphates.

226 The interaction of rhodopsin-attached phosphates with Lys-14 and Lys-15 in beta-stran

227 ich are then replaced by more distant ribbon-attached vesicles, creating opportunities for new vesicl

228 Factor assists in the maturation of ribosome-attached nascent chains by acting as both a holdase and

229 wobble, and Fourier analysis of moving, ring-attached beads corroborates this prediction.

230 compartments: surface biological crust, root-attached, and the broader rhizosphere.

231 europathic rat dorsal horn, with dorsal root-attached preparations, soluble FKN (sFKN) contents are i

232 correlated with mechanical states of spindle-attached kinetochores, provided a nanometer-scale map of

233 e resistance to many DUBs of long, substrate-attached Lys(48)-linked chains helps ensure that protein

234 nocolumns, either free-standing or substrate-attached, with a hydrogel layer.

235 not plectin, colocalize along the substratum-attached surface.

236 nts to the geometrical features of a surface-attached biomolecule.

237 bility of this DNA to hybridize to a surface-attached complementary strand.

238 te the assembly and utilization of a surface-attached double-stranded DNA catenane composed of two in

239 type III secretion system (TTSS), a surface-attached needle-like complex that injects cytotoxins dir

240 Although surface-attached bacteria often experience strong chemical gradi

241 g evaluations on both planktonic and surface-attached bacteria provided new insights on these distinc

242 tches between free-living motile and surface-attached sessile lifestyles.

243 Biofilms are surface-attached agglomerations of microorganisms embedded in an

244 ng planktonic bacteria, biofilms are surface-attached communities of slow- or non-replicating bacteri

245 Pili are surface-attached, fibrous virulence factors that play key roles

246 cies.Most bacteria live in biofilms, surface-attached communities encased in an extracellular matrix.

247 Biofilms, surface-attached communities of bacteria encased in an extracell

248 nly to a certain depth (ca. 2 nm) by surface-attached Ni2+.

249 ural systems, bacteria form complex, surface-attached communities known as biofilms.

250 Microbes commonly live in dense surface-attached communities where cells layer on top of one ano

251 ting relevance of this mechanism for surface-attached platelets under physiological flow.

252 the environment, most bacteria form surface-attached cell communities called biofilms.

253 Many species of bacteria form surface-attached communities known as biofilms.

254 Bacteria form surface-attached communities, known as biofilms, which are centr

255 marine environments, where it forms surface-attached communities commonly described as biofilms.

256 omastigote morphology but also forms surface-attached haptomonads and amastigotes.

257 hedding of extracellular matrix from surface-attached biofilms generates a sieve-like network that ca

258 m avian H5N1 influenza virus to gold surface-attached single-stranded DNA oligonucleotide probes.

259 For the heterogeneous, surface-attached material generated from [Cp*Ir(H(2)O)(3)](2+),

260 s to the formation of hyperbiofilms, surface-attached communities reaching high density.

261 Microbes are predominantly found in surface-attached and spatially structured polymicrobial communit

262 Many bacteria on earth exist in surface-attached communities known as biofilms.

263 ression in suspended cultures and in surface-attached microcolonies revealed that QS onset in both sy

264 n nature, bacteria primarily live in surface-attached, multicellular communities, termed biofilms (1-

265 However, how individual surface-attached bacteria self-organize into microcolonies, the

266 force-free association of individual surface-attached fibrinogen and alphaIIbbeta3 molecules and forc

267 ordinates subunit self-assembly into surface-attached fibers.

268 cellular state and the multicellular surface-attached community state (biofilm) by a range of mechani

269 ufficient to induce virulence in non-surface-attached cells, suggesting that PilY1 mediates surface m

270 n method, single cells that were not surface-attached were isolated with a 100 +/- 0% efficiency and

271 rength and probability of binding of surface-attached antibodies with oligomeric PF4 to simulate inte

272 We discovered that accumulation of surface-attached biofilm has little effect on flow through such

273 The crowding of surface-attached biomolecules may thus be a richer, more complex

274 iofilms are microbial communities of surface-attached cells embedded in a self-produced extracellular

275 f biofilms--organized communities of surface-attached cells--but physiologically and genetically defi

276 nt planktonic cells or as members of surface-attached communities called biofilms.

277 Biofilms are composed of surface-attached microbial communities.

278 en described including retraction of surface-attached pili, secretion of polysaccharides, or movement

279 te thermodynamic and kinetic data on surface-attached antibodies with their respective antigens.

280 Like all sessile organisms, surface-attached communities of bacteria known as biofilms must

281 n which surface shear stress orients surface-attached P. aeruginosa cells along the flow direction, c

282 ents that cannot be reached by other surface-attached bacteria.

283 ngth and head number (N) moving over surface-attached F-actin in vitro.

284 cleotide conjugates with the silicon surface-attached complementary sequence.

285 owding on the stability of a simple, surface-attached DNA stem-loop.

286 simulate the growth of a two-species surface-attached community where food (resource) is traded for d

287 ctin glycoprotein ligand 1), and the surface-attached chemokine fractalkine, all implicated in leukoc

288 dominant life stage is formed by the surface-attached colony that shows many complex features: coloni

289 surfaces and hybridization with the surface-attached DNA, new methodologies have been developed.

290 PS), while the binding of cocaine to surface-attached MN4 was evaluated using the electromagnetic pie

291 bacteria from single motile cells to surface-attached multicellular communities.

292 repeatedly brought into contact with surface-attached purified alphaIIbbeta3.

293 ia, including persister cells within surface-attached biofilms and slow-growing pathogens lead to chr

294 Pneumococci were present within surface-attached biofilms in coinfected animals, and a greater p

295 responsible for transferring the N-terminus-attached catalytic domain across the endosomal membrane

296 d of membrane-attached (t-SNARE) and vesicle-attached (v-SNARE) proteins that zipper together to form

297 glycosylated mucin-like domain of the virion-attached glycoprotein (GP).

298 V entry requires the interplay of the virion-attached hemagglutinin-esterase and fusion glycoproteins

299 mucin-like domain of the Ebola virus virion-attached glycoprotein (GP) and protects animals against

300 sive matrix molecules), in which a cell wall-attached stalk enables long range interactions via its a