ライフサイエンスコーパス: -facing

1 Alanine mutation of the Ago2-facing Lys56 in eIF1A impairs RNAi activities in human c

2 e hydrolysis at specific sites near the AuNP-facing terminus of siRNAAR, which were different from th

3 the ADK5b epitope spans both the 5-fold axis-facing wall of the 3-fold protrusion and portions of the

4 or quills that feature microscopic backward-facing deployable barbs that are used in self-defense.

5 inations of engineering structures (backward-facing steps forming d-type ribs on the porous surface o

6 ng it close the mouth, whereas the backwards-facing marginal serrations of the bivalved carapace may

7 In Xenopus, the blastocoel-facing, basolateral surfaces where signaling interaction

8 tein is localized at both basolateral (blood-facing) and apical (saliva-facing) membranes of salivary

9 w report that PTs express RPTPgamma on blood-facing membranes.

10 y of podosomal belts that segregate the bone-facing ruffled membrane from other membrane domains.

11 l agents, and mutations at predicted channel-facing positions modulate this effect.

12 that regard, with both clinical and consumer-facing analysis tools relying on this self-contained des

13 claim to be both domain-facing and consumer-facing have to pass the test of portability across the v

14 PMAT protein is localized to the apical (CSF-facing) membrane of the CP epithelium, consistent with a

15 Pores or stomata present on CSF-facing leptomeningeal cells ensheathing blood vessels in

16 e syringe, the injectisome, with a cytoplasm-facing translocase channel.

17 ast three different conformations: cytoplasm-facing, occluded, and lumen-facing.

18 anged in two domains, with a large cytoplasm-facing funnel and a smaller periplasm-facing funnel.

19 tors of VMAT2: reserpine binds the cytoplasm-facing conformation, and tetrabenazine binds the lumen-f

20 o shift the equilibrium toward the cytoplasm-facing conformation, emulating the effect of protonation

21 The lumen- to cytoplasm-facing transition, facilitated by protonation of at leas

22 sensing, may line the channel's cytoplasmic-facing funnel surface, stabilize the channel's overall s

23 nger (Vcx1) at 2.3 A resolution in a cytosol-facing, substrate-bound conformation.

24 CAX family, and it describes the key cytosol-facing conformation of the CaCA superfamily, providing t

25 The cytosol-facing conformations within the CaCA superfamily are unk

26 phosphate (DSMP) intermediate by the cytosol-facing inner membrane kinase LpxK, a member of the P-loo

27 The cytosol-facing membranes of cellular organelles contain proteins

28 d a group of missense mutations in cytosolic-facing regions of the Frizzled receptor that block Dishe

29 molecular identities between their cytosolic-facing surface membranes and their interior?

30 are libraries with a claim to be both domain-facing and consumer-facing have to pass the test of port

31 rypanosomes orient upstream while downstream-facing cells tumble within the same streamline.

32 owest rates of warming evident on north-east-facing slopes.

33 eems to be unaffected by operation, exterior-facing and feedstream-introduced iron was found in two f

34 (+) congener), all captured in extracellular-facing and drug-bound states.

35 structure of an outer-membrane extracellular-facing deca-haem terminus for such a module has recently

36 , His(17) or His(19), or other extracellular-facing histidines to alanine residues did not abolish th

37 mics of the protonation of the extracellular-facing gating Glu (Ex) and Cl(-) binding to the external

38 The extracellular-facing H(+) transfer glutamate becomes directly exposed

39 eir retina, ants wait until an expected goal-facing phase of the zigzag before turning to correct the

40 el, and EPR spectra from labeled hydrocarbon-facing sites in BtuB may reflect this asymmetry.

41 ch associates with intermembrane space (IMS)-facing membrane leaflets.

42 enerated by Cld1p must be transported to IMS-facing membrane leaflets to gain access to tafazzin, ide

43 Two are in different inside-facing states.

44 (outward)-facing and intracellular (inward)-facing conformations, whereby substrate binding sites be

45 of the uracil transporter UraA in an inward-facing conformation allowed us to identify the anion-bin

46 ts, stabilizing the transporter in an inward-facing conformation and preventing the structural rearra

47 dermidis glucose/H(+) symporter in an inward-facing conformation at 3.2-A resolution.

48 Recently, an inward-facing conformation of LeuT was solved revealing an unex

49 X_Mj structure, an atomic model of an inward-facing conformation was generated showing similar pathwa

50 protonated state of YfkE exhibits an inward-facing conformation with a large hydrophilic cavity open

51 g domains of the transporter adopt an inward-facing conformation with the two nucleotide-binding doma

52 The structure shows UapA in an inward-facing conformation with xanthine bound to residues in t

53 side and an open cytoplasmic side (an inward-facing conformation).

54 reveal an unprecedented closed and an inward-facing conformation, respectively.

55 a series of crystal structures in an inward-facing conformation.

56 site when the transporter rests in an inward-facing conformation.

57 substrate and from isomerizing to an inward-facing conformation.

58 nd sealed on the periplasmic side (an inward-facing conformer).

59 ty phosphate transporter, PiPT, in an inward-facing occluded state, with bound phosphate visible in t

60 ating that the transporter lies in an inward-facing open conformation.

61 ansition from an outward-facing to an inward-facing state of the transporter.

62 tate, our model is consistent with an inward-facing state, suggesting that the conformational change

63 Can1 is promoted by transition to an inward-facing state.

64 sport domain between the outward- and inward-facing conformational states, sparing the equivalent ste

65 rystal structures in the outward- and inward-facing conformations of a glutamate transporter homolog

66 ured in outward-facing, occluded, and inward-facing conformations.

67 free and ions-only bound outward- and inward-facing conformations.

68 Whereas outward- and inward-facing GltPh conformations are nonconductive, lateral mo

69 ng occluded with substrate bound, and inward-facing open).

70 uctures of outward-facing Sav1866 and inward-facing Pgp.

71 or Glt(Ph) in both outward-facing and inward-facing states by taking advantage of significant advance

72 transition between outward-facing and inward-facing states) scales.

73 tion of E374 in both the outward- and inward-facing states, hence the proton is not released in the e

74 tions, including both the outward and inward-facing states.

75 erence between the outward-facing and inward-facing structures resolved for the archaeal aspartate tr

76 leotide binding domains (NBDs) apart; inward-facing] and closed (NBDs close; outward-facing) conforma

77 outward-open conformation as well as inward-facing conformations.

78 structure, TmrAB adopts an asymmetric inward-facing state, and we show that the C-terminal helices, a

79 pathway when the transporter becomes inward-facing.

80 making iterative transitions between inward-facing (E1) and outward-facing (E2) conformations.

81 e to LeuT, promotes an outward-closed/inward-facing conformation of the transporter and increases upt

82 the activation of two cytoplasmically inward-facing channels: the plasma membrane, Cch1p, and the vac

83 previously described nucleotide-free, inward-facing and nucleotide-bound, outward-facing conformation

84 lubilized TmrAB in a nucleotide-free, inward-facing conformation by single-particle electron cryomicr

85 intact structure is available both in inward-facing and outward-facing conformations.

86 he crystal structure of XylE in a new inward-facing open conformation, allowing us to visualize the r

87 Furthermore, simulations of inward-facing LeuT with Na(+) ions and substrate bound suggest

88 xpected to increase the proportion of inward-facing transporters, such as the presence of GABA togeth

89 xpected to increase the proportion of inward-facing transporters, whereas the reactivity of the mutan

90 lux involves changes between the open inward-facing (NBDs apart, extracellular loops (ECLs) close tog

91 crystal structure of MsbA in the open inward-facing conformation.

92 In this study, an open, inward-facing conformation of Pho84 was used to study the relea

93 ational change in Pho84 from an open, inward-facing state to an occluded state.

94 esting that Mhp1 adopts predominantly inward-facing conformations.

95 sidues selected to track the putative inward-facing to outward-facing transition.

96 iving (outward-facing) to -releasing (inward-facing) state appears to be a determinant of its affinit

97 binding site to the cytoplasmic side (inward-facing, open NBD conformation).

98 resolve the backbone dynamics in the inward-facing (IF) and outward-facing (OF) states by analyzing

99 enhanced solvent accessibility in the inward-facing (relative to the outward-facing) form.

100 sette (ABC) transporter, BmrA, in the inward-facing (resting state) and outward-facing (ATP-bound) co

101 Our results reveal that the inward-facing and outward-facing Mhp1 crystal structures repres

102 By trapping NCX1.1 in the inward-facing configuration, we have mapped two differently siz

103 ucose EIIC superfamily, bcChbC in the inward-facing conformation and bcMalT in the outward-facing con

104 he R- and S-enantiomers tolerated the inward-facing conformation better than cocaine, which was furth

105 a positions from ICD2 residues in the inward-facing conformation of a related transporter, MsbA.

106 nic binding of K(+) or of H(+) to the inward-facing conformation of SERT in (i) cancelling out the el

107 of the two membrane domains from the inward-facing conformation to the outward-facing conformation.

108 erse the conformation of MalFG to the inward-facing conformation, a step essential for release of mal

109 MalFG may be naturally stable in the inward-facing conformation, or the ABC domain may catalyze the

110 eveals an unexpected homodimer in the inward-facing conformation.

111 s performed on a peptide bound to the inward-facing models.

112 we propose a structure model for the inward-facing NCX_Mj.

113 Pho84 transport model is based on the inward-facing occluded crystal structure of the Pho84 homologue

114 dynamics of membrane-bound Pgp in the inward-facing state and found that Pgp adopts an unexpectedly w

115 uired to reset the transporter to the inward-facing state are initiated by sequential hydrolysis of t

116 until maltose triggers return to the inward-facing state for substrate and Pi release.

117 gaine (a DAT ligand selective for the inward-facing state) and/or pifithrin-mu (an HSP70 inhibitor) r

118 d converts the membrane domain to the inward-facing state.

119 for the release of the proton in the inward-facing state.

120 ing by facilitating transition to the inward-facing state.

121 resolved to date for CaiT, all in the inward-facing state: CRN-bound (with four CRNs per subunit), GB

122 cess to the ion-binding site from the inward-facing surface of the protein.

123 omains during the transition from the inward-facing to outward-facing conformations.

124 he structural differences between the inward-facing YfkE and the outward-facing NCX_Mj suggest that t

125 we present crystal structures of the inward-facing, intermediate, and outward-facing states of a con

126 Together, these inward-facing conformational snapshots of P-gp demonstrate a ra

127 to investigate the properties of this inward-facing conformation in relation to transport by LeuT wit

128 esent the crystal structures of three inward-facing conformations of mouse P-gp derived from two diff

129 d during the passage from outward- to inward-facing state (holo-occluded), and another, substrate-fre

130 increases in an intermediate close to inward-facing state.

131 ctural isomerization from outward- to inward-facing states.

132 toplasmic gate during the outward- to inward-facing transition of apo MsbA is found to be disfavored

133 we rigorously sample the outward- to inward-facing transition path ensemble.

134 SUR1-ABC core is found in an unusual inward-facing conformation whereby the two nucleotide binding d

135 opyridines is located on the external, lipid-facing surface of the pore module, positioned at the int

136 sin by tryptophan substitution of four lipid-facing residues in transmembrane helix 4 (TM4) that is k

137 that residues located on the "outer" (lipid-facing) surface of the transmembrane (TM) receptor core

138 show that the introduction of a single lipid-facing arginine residue near the middle of the beta barr

139 e energy for all 20 amino acids at the lipid-facing interface (DeltaDeltaG(0)w,i(phi)) and the protei

140 are most favorably transferred to the lipid-facing interface, whereas charged and polar groups displ

141 o tryptophan substitution, whereas the lipid-facing regions of TM3 and TM4 were variably tolerant.

142 h the partitioning free energy for the lipid-facing residue and inversely with the protein-facing res

143 ing process of an OMP is driven by the lipid-facing residues in its hydrophobic core, and its NC-IN t

144 to-bilayer transfer free energy of the lipid-facing residues in their transmembrane regions.

145 oteins to the complete redesign of the lipid-facing surface of Escherichia coli OmpA.

146 and the design rules that govern this lipid-facing surface are poorly understood.

147 inding site in the cavity, adjacent to lipid-facing fenestrations proposed to be the portals for drug

148 erally applicable to drug targets with lipid-facing binding sites.

149 tions: cytoplasm-facing, occluded, and lumen-facing.

150 sically disordered regions on bZIP28's lumen-facing tail.

151 formation, and tetrabenazine binds the lumen-facing conformation.

152 he membrane-embedded F(o) part to the matrix-facing central stalk of F(1).

153 After its synthesis on the matrix-facing leaflet of the inner membrane (IM), CL undergoes

154 deling, Cld1p, is associated with the matrix-facing leaflet of the mitochondrial IM.

155 We identified residues in the matrix-facing region of Tim17 involved in binding of the import

156 red linker that becomes helical and membrane-facing in the open form of the AP2 clathrin adaptor comp

157 at active Msp1 contains a conserved membrane-facing surface adjacent to a central pore.

158 The membrane-facing surface of the COPII proteins (especially SEC23-S

159 at well-defined locations on their membrane-facing surface.

160 emical differences between WSOM from a north-facing slope, south-facing slope, and riparian zone, the

161 S = SFS) abuts the forested "European" north-facing slope (ES = NFS).

162 s inhabiting the colder and more humid north-facing slope carried about 6% more transposable elements

163 re abundant in flies derived from one (north-facing) of the slopes.

164 frican" slope (AS)], abutting with the north-facing slope forested south-European continent ["Europea

165 One protomer is in the outside-facing state.

166 transitions between extracellular (outward)-facing and intracellular (inward)-facing conformations,

167 in the protein being unable to adopt outward-facing conformations or in a global destabilization of s

168 We found a novel high affinity outward-facing conformational state of the human SERT induced by

169 that R60A preferentially assumes an outward-facing (OF) conformation through compensatory intracellu

170 mport, and a crystal structure in an outward-facing conformation has been reported.

171 ting that sodium binding promotes an outward-facing conformation of the transporter.

172 om Methanococcus jannaschii shows an outward-facing conformation suggesting a hypothesis of alternati

173 site to T356) with a Met promotes an outward-facing conformation upon substrate binding.

174 on of the TMD, from an inward- to an outward-facing conformation, is driven exclusively by ATP hydrol

175 1.9 angstrom crystal structure in an outward-facing conformation.

176 en interact with DAT to stabilize an outward-facing DAT conformation and enhance cocaine binding.

177 Electrons are channeled from an outward-facing formate dehydrogenase via menaquinones to a fumar

178 An outward-facing model has also been proposed based on biochemical

179 In silico modeling revealed an outward-facing molecule, with helices 1, 2, 4, 7, 8, and 10 havi

180 symmetric conformations, yielding an outward-facing occluded state.

181 The structure is locked in an outward-facing open conformation by an inhibitor.

182 il of ATP in this structure is in an outward-facing position pointing away from the active site.

183 odel indicates that it represents an outward-facing state, our model is consistent with an inward-fac

184 (NBDs) leads to the formation of an outward-facing state.

185 a conformational transition from an outward-facing to an inward-facing state of the transporter.

186 In the substrate-bound state, an outward-facing transporter conformation is required for substrat

187 he inward-facing (resting state) and outward-facing (ATP-bound) conformations.

188 tions between inward-facing (E1) and outward-facing (E2) conformations.

189 s), which switch between inward- and outward-facing (IF, OF) orientations.

190 transitions between inward- (IF) and outward-facing (OF) states ('alternating-access' mechanism).

191 namics in the inward-facing (IF) and outward-facing (OF) states by analyzing purified preparations of

192 erstood to represent the inward- and outward-facing conformational states of the transporter.

193 P and alternates between inward- and outward-facing conformations during maltose transport.

194 t the transition between inward- and outward-facing conformations during the catalytic cycle of BmrA.

195 ge the energetics of the inward- and outward-facing conformations of Mhp1.

196 ternating access between inward- and outward-facing conformations of the substrate-loaded transporter

197 esent the transporter in inward- and outward-facing conformations that could represent initial and fi

198 that alternates between inward- and outward-facing conformations to capture and force substrates out

199 available both in inward-facing and outward-facing conformations.

200 nes by switching between inward- and outward-facing conformations.

201 ts reveal that the inward-facing and outward-facing Mhp1 crystal structures represent sampled interme

202 the inward-facing, intermediate, and outward-facing states of a concentrative nucleoside transporter

203 erconversion between the inward- and outward-facing states of EmrE varies over 3 orders of magnitude.

204 mational changes between inward- and outward-facing states.

205 tween conformations with inward- and outward-facing substrate-binding sites in response to engagement

206 l structures of MalFGK2, inward- and outward-facing, show that the transporter is sealed against ions

207 sure) and global (transition between outward-facing and inward-facing states) scales.

208 erformed to date for Glt(Ph) in both outward-facing and inward-facing states by taking advantage of s

209 rter in a sodium- and chloride-bound outward-facing open conformation.

210 uded and competitive inhibitor-bound outward-facing states have advanced our mechanistic understandin

211 inward-facing and nucleotide-bound, outward-facing conformations of ABC exporters and which binds AT

212 ward-facing] and closed (NBDs close; outward-facing) conformations.

213 ECLs) close together) and the closed outward-facing (NBDs close together, ECLs apart) conformations.

214 ed construction of a high-confidence outward-facing Pgp atomic model based on crystal structures of o

215 as been crystallized in three forms (outward-facing open, outward-facing occluded with substrate boun

216 ded SBP docks to the nucleotide-free outward-facing conformation of the transporter.

217 e medium and normal skin to generate outward-facing gradients.

218 ugh to form a disulfide bond only in outward-facing and early intermediate conformations of the homol

219 In outward-facing crystal structures of the bacterial homodimeric A

220 The high degree of similarity in outward-facing structures across evolution of ABC transporters a

221 , the LeuT-fold has been captured in outward-facing, occluded, and inward-facing conformations.

222 strate a predominant lateral, mainly outward-facing, plasma membrane location for TWD1 in the root ep

223 shift in equilibrium to favor a more outward-facing conformation of SERT can explain the reduced turn

224 hat were sensitized to detect a more outward-facing conformation of SERT.

225 o guide site-directed mutagenesis of outward-facing hydrophobic residues within the transmembrane reg

226 model based on crystal structures of outward-facing Sav1866 and inward-facing Pgp.

227 quence of an increased proportion of outward-facing transporters.

228 in three forms (outward-facing open, outward-facing occluded with substrate bound, and inward-facing

229 binding site, but also in a putative outward-facing selective gate.

230 configures from substrate-receiving (outward-facing) to -releasing (inward-facing) state appears to b

231 essibility of the drug-binding site (outward-facing, closed NBD conformation), and ATP hydrolysis lea

232 te that occupation of Na2 stabilizes outward-facing conformations presumably through a direct interac

233 denced by the difference between the outward-facing and inward-facing structures resolved for the arc

234 ding of transition metal ions to the outward-facing apo state of DAT and a reciprocal interaction of

235 a conformational change in which the outward-facing cavity in FucP closes, thereby bringing Trp38 and

236 positioned on opposing faces of the outward-facing cavity walls, are replaced with Tyr or Phe, and r

237 with a fully atomistic model of the outward-facing conformation in membranes, the insight into Pgp c

238 close proximity to each other in the outward-facing conformation of GAT-1.

239 der conditions expected to favor the outward-facing conformation of the transporter.

240 xhibits aqueous accessibility in the outward-facing conformation only.

241 and liganded MalE, both inducing the outward-facing conformation trapped in the crystal with open Mal

242 state MalE stabilizes MalFGK2 in the outward-facing conformation until maltose triggers return to the

243 In the outward-facing conformation, the transmembrane subunit MalF bind

244 acing conformation and bcMalT in the outward-facing conformation, were previously solved.

245 he inward-facing conformation to the outward-facing conformation.

246 In dimerization, the outward-facing ligands rotate in a sense opposite to the forming

247 tween the inward-facing YfkE and the outward-facing NCX_Mj suggest that the conformational transition

248 For the outward-facing residues of the beta-barrel within regions of sim

249 ng step where SERT may return to the outward-facing state in a KCl- or HCl-bound form.

250 pidly from the inward- than from the outward-facing state of DAT.

251 ed to protonate Gluex and enrich the outward-facing state, a residue ~20 A away from Gluex, near the

252 In the outward-facing state, S1 robustly binds alanine and regulates su

253 n the inward-facing (relative to the outward-facing) form.

254 transition from the inward-facing to outward-facing conformations.

255 are transferred from menaquinones to outward-facing CprA, via an as-yet-unidentified membrane complex

256 and a global reconfiguration back to outward-facing state to resume the transport cycle.

257 s a reorientation from an inward- to outward-facing state.

258 nformational change from occluded to outward-facing states is unusually simple, involving only the ro

259 track the putative inward-facing to outward-facing transition.

260 labeling, indicating a shift toward outward-facing conformations that can be interpreted using conve

261 ion of a previously uncharacterized 'outward-facing open' state, and highlight the relevance of globa

262 bout 100 contractile structures with outward-facing baseplates, linked by tail fibers and a dynamic h

263 de variety of clinical scenarios and patient-facing governance where only part of the VCF data is fit

264 agues highlight how the expansion of patient-facing electronic health record portals could exacerbate

265 age consultation duration, and total patient-facing clinical workload in English general practice.

266 ural architecture of an alternate, periplasm-facing state.

267 oplasm-facing funnel and a smaller periplasm-facing funnel.

268 may be a redeeming factor for Ta as a plasma-facing material.

269 al integrity of leading candidate for plasma-facing materials, tungsten (W), in future nuclear fusion

270 s work is to assess Ta as a potential plasma-facing material for future fusion reactors in terms of i

271 arge heat fluxes onto the surrounding plasma-facing components (PFCs).

272 kamak-relevant conditions on tungsten plasma-facing materials in a magnetic fusion energy device.

273 tance anomalies are concentrated on poleward-facing slopes and are spatially collocated with areas of

274 In the modeled channel, pore-facing regions of TM1 and TM2 were highly sensitive to t

275 dification of BK S6 residues identifies pore-facing residues that occur at different linear positions

276 the anion-selective ones) of a ring of pore-facing carboxylates, mutational studies have identified

277 ticularly at Ser12, indicating that the pore-facing serine residues in BM2 mediate proton relay to th

278 olvement of ionized side chains-whether pore-facing or buried-rather than backbone atoms and propose

279 ressed early between anterior- and posterior-facing wounds.

280 signaling in intact animals and at posterior-facing wounds.

281 amic contributions of the lipid- and protein-facing residues in the transmembrane protein beta-signal

282 ace (DeltaDeltaG(0)w,i(phi)) and the protein-facing interface (DeltaDeltaG(0)w,i(pi)) residues and fo

283 acing residue and inversely with the protein-facing residue.

284 nserved regions in the endoplasmic reticulum-facing loops with a role in the opening and closing of t

285 asolateral (blood-facing) and apical (saliva-facing) membranes of salivary gland acinar cells, sugges

286 ter is an internally cooled, 14-French, side-facing catheter, integrated with A-mode ultrasound guida

287 y C1-oxidizing LPMO9s, access to the solvent-facing axial coordination position is restricted by a co

288 tropical, xeric, savannoid, "African" south-facing slope (AS = SFS) abuts the forested "European" no

289 ements than those from the hot and dry south-facing slope, in parallel to a suite of other genetic an

290 otically, representing the dry and hot south-facing slope savannoid-African continent ["African" slop

291 etween WSOM from a north-facing slope, south-facing slope, and riparian zone, there were clear differ

292 ates non-integrally with intermembrane space-facing membranes and assembles in a range of complexes.

293 lls were more often in the connective tissue-facing side of the epithelium.

294 he vertices of a hexagonal lattice in a "two-facing-two" configuration surrounding a ring of alternat

295 g a level of performance sufficient for user-facing applications.

296 ices are deployed as containers and the user-facing interfaces as web-based applications.

297 appendages that bear well-developed ventral-facing spines, paired dorsal spines on the trunk, and po

298 ltaT 0-50 for south-, north-, east- and west-facing sides of buildings were highest on sunny days in

299 d 0.20 m for south-, north-, east-, and west-facing walls.

300 ts on temperature of solar radiation on west-facing slopes.