ライフサイエンスコーパス: 2-oxoglutarate

1 nic acid, N-acetylglucosamine, and decreased 2-oxoglutarate.

2 small effectors, most notably glutamine and 2-oxoglutarate.

3 does not prevent the binding of the cofactor 2-oxoglutarate.

4 e trimethylamine N-oxide (TMAO), citrate and 2-oxoglutarate.

5 ethylase activity dependent on both iron and 2-oxoglutarate.

6 w carbon/nitrogen and depleted in starch and 2-oxoglutarate.

7 es the oxidative deamination of glutamate to 2-oxoglutarate.

8 amino-terminal GAF domain of NifA that binds 2-oxoglutarate.

9 ng the release of 14CO2 from labeled [1-14C]-2-oxoglutarate.

10 ible oxidative deamination of L-glutamate to 2-oxoglutarate.

11 ylate, Ala:glyoxylate, Glu:pyruvate, and Ala:2-oxoglutarate.

12 the oxidative deamination of l-glutamate to 2-oxoglutarate.

13 tive succinylation by E1o in the presence of 2-oxoglutarate.

14 e NIFL-NIFA system is directly responsive to 2-oxoglutarate.

15 sugar phosphate levels, and lower content of 2-oxoglutarate.

16 he dioxygenase cofactor iron and cosubstrate 2-oxoglutarate.

17 eoxy-d-GlcNAc to form UDP-4-amino-FucNAc and 2-oxoglutarate.

18 interactions are modulated by ADP, ATP, and 2-oxoglutarate.

19 droxylases via enzyme-catalysed oxidation to 2-oxoglutarate.

20 their ability to bind the effector molecules 2-oxoglutarate (2-OG) and ATP or ADP.

21 These 2-oxoglutarate (2-OG) and non-heme iron-dependent oxygen

22 es co-regulated cancer genes associated with 2-oxoglutarate (2-OG) and succinate metabolism, includin

23 talytic domain in complex with the substrate 2-oxoglutarate (2-OG) and the inhibitor N-oxalylglycine

24 ynthetase/glutamate synthase system requires 2-oxoglutarate (2-OG) as a carbon precursor.

25 Levels of 2-oxoglutarate (2-OG) reflect nitrogen status in many ba

26 Recently, members of the 2-oxoglutarate (2-OG)-dependent dioxygenase family have

27 ins (JBP1 and JBP2) homologous to the Fe(2+)/2-oxoglutarate (2-OG)-dependent dioxygenase superfamily

28 Iron (II)/2-oxoglutarate (2-OG)-dependent oxygenases catalyse oxid

29 of sigE and that this binding is enhanced by 2-oxoglutarate (2-OG).

30 hich was corrected by provision of exogenous 2-oxoglutarate (2-OG).

31 of the enzyme in complex with the substrate 2-oxoglutarate (2-OG).

32 2-Oxoglutarate (2OG) and Fe(II)-dependent oxygenase doma

33 ALKBH5 is a 2-oxoglutarate (2OG) and ferrous iron-dependent nucleic

34 range of Bacteria and Archaea sense cellular 2-oxoglutarate (2OG) as an indicator of nitrogen limitat

35 h NrpR, nifOR(1), nifOR(2), and the effector 2-oxoglutarate (2OG) combine to regulate nif expression,

36 al human homologues belong to a subfamily of 2-oxoglutarate (2OG) dependent oxygenases (2OG oxygenase

37 The human 2-oxoglutarate (2OG) dependent oxygenases belong to a fa

38 member of the Jumonji C family of Fe(II) and 2-oxoglutarate (2OG) dependent oxygenases.

39 he presence of NifI(1) and NifI(2), and that 2-oxoglutarate (2OG), a potential signal of nitrogen lim

40 the MLL gene in acute myeloid leukemia, is a 2-oxoglutarate (2OG)- and Fe(II)-dependent enzyme that c

41 translocation (TET) proteins are Fe(II)- and 2-oxoglutarate (2OG)-dependent dioxygenases that success

42 Escherichia coli DNA repair enzyme AlkB is a 2-oxoglutarate (2OG)-dependent Fe(2+) binding dioxygenas

43 FTO is a 2-oxoglutarate (2OG)-dependent N-methyl nucleic acid dem

44 ember of the mononuclear nonheme Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenase superfamily.

45 Mononuclear non-heme Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenases comprise a lar

46 2-Oxoglutarate (2OG)-dependent oxygenases have important

47 enome sequences predict the presence of many 2-oxoglutarate (2OG)-dependent oxygenases of unknown bio

48 The JmjC KDMs are Fe(II) and 2-oxoglutarate (2OG)-dependent oxygenases, some of which

49 f prolyl and lysyl residues, as catalyzed by 2-oxoglutarate (2OG)-dependent oxygenases, was first ide

50 tion hydroxylation as catalyzed by iron- and 2-oxoglutarate (2OG)-dependent prolyl and asparaginyl hy

51 The roles of 2-oxoglutarate (2OG)-dependent prolyl-hydroxylases in eu

52 talyze the interconversion of isocitrate and 2-oxoglutarate (2OG).

53 cytosolic alpha-ketoglutarate, also known as 2-oxoglutarate (2OG).

54 ascorbate, and the Kreb's cycle intermediate 2-oxoglutarate (2OG).

55 ppression increased levels of the metabolite 2-oxoglutarate (2OG).

56 PLOD2 (procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2) hydroxylates lysine resi

57 inhibition is antagonised by the binding of 2-oxoglutarate, a key metabolic signal of the carbon sta

58 The electron acceptor for 2-oxoglutarate:acceptor oxidoreductase was determined to

59 tarate respiration is mediated by the enzyme 2-oxoglutarate:acceptor oxidoreductase; mutagenesis of t

60 ially vulnerable, as it employs pyruvate and 2-oxoglutarate:acceptor oxidoreductases (Por and Oor), w

61 One such enzyme is the 2-oxoglutarate (alpha-ketoglutarate) oxidoreductase (OOR

62 logical role of the NADH-dependent glutamine-2-oxoglutarate aminotransferase (NADH-GOGAT) enzyme was

63 the urea and glutamine synthetase/glutamine 2-oxoglutarate aminotransferase pathways and redirected

64 rs, nitrate/nitrite reductases and glutamine:2-oxoglutarate aminotransferase.

65 tein phosphotransferase, and diaminobutyrate-2-oxoglutarate aminotransferase.

66 With the carbon skeleton provided by 2-oxoglutarate, ammonia/ammonium (NH(4)(+)) is assimilat

67 ion using the isoquinolone Roxadustat or the 2-oxoglutarate analog dimethyloxalylglycine (DMOG).

68 prolyl hydroxylase inhibitors are lipophilic 2-oxoglutarate analogues (2OGAs) that are widely taken u

69 A set of human 2-oxoglutarate analogues were screened using a unified a

70 o an evolutionarily conserved superfamily of 2-oxoglutarate and Fe(II)-dependent dioxygenases that me

71 te shunt is a major contributor to flux from 2-oxoglutarate and glutamate to succinate in Synechocyst

72 tors (HIFs) are principally regulated by the 2-oxoglutarate and Iron(II) prolyl hydroxylase (PHD) enz

73 Unlike classical 2-oxoglutarate and iron-dependent dioxygenases, which in

74 plasmic reticulum and belong to the group of 2-oxoglutarate and iron-dependent dioxygenases.

75 275Q and R275W mutants were assayed for both 2-oxoglutarate and phytanoyl-CoA oxidation.

76 es of ADP-stimulated (State 3) and uncoupled 2-oxoglutarate and succinate oxidation increased in para

77 tinguish between the C5-carboxylate group of 2-oxoglutarate and the epsilon-ammonium group of l-lysin

78 mpounds (iron, ascorbate, hydrogen peroxide, 2-oxoglutarate, and succinate) influenced by cellular ox

79 beta-Phe, (R)-3-amino-5-methylhexanoic acid, 2-oxoglutarate, and the inhibitor 2-aminooxyacetic acid,

80 a form that contained iron, the co-substrate 2-oxoglutarate, and the reaction product of EctD, 5-hydr

81 that Jumonji domain-containing 4 (Jmjd4), a 2-oxoglutarate- and Fe(II)-dependent oxygenase, catalyze

82 ydroxymethyl-cytosine (hmC) by the action of 2-oxoglutarate- and Fe(ii)-dependent oxygenases of the T

83 The Jumonji C lysine demethylases (KDMs) are 2-oxoglutarate- and Fe(II)-dependent oxygenases.

84 arboxyl-terminal domain corresponding to the 2-oxoglutarate- and iron-dependent dioxygenase domains s

85 s (the flavin-dependent KDM1 enzymes and the 2-oxoglutarate- and oxygen-dependent JmjC KDMs, respecti

86 inishes AmtB/GlnK association, and sites for 2-oxoglutarate are evaluated.

87 ses depends on iron as the activating metal, 2-oxoglutarate as a co-substrate, and ascorbic acid as a

88 requirement for iron(II) as a co-factor and 2-oxoglutarate as a co-substrate.

89 xoadipate and pyruvate substitute poorly for 2-oxoglutarate as a cosubstrate.

90 using flavin (amine oxidases) or Fe(II) and 2-oxoglutarate as cofactors (2OG oxygenases) has changed

91 of alpha-ketoglutarate (alternatively termed 2-oxoglutarate) as a co-substrate in so many oxidation r

92 Mutation of Arg-275 resulted in impaired 2-oxoglutarate binding.

93 e AML-associated mutations in the Fe(2+) and 2-oxoglutarate-binding residues increased the Km values

94 e-dependent dioxygenases, putative iron- and 2-oxoglutarate-binding residues, typical of such enzymes

95 ll core with both Fe(II) and the cosubstrate 2-oxoglutarate bound in the active site.

96 acids, AOX1A and AOX1D are both activated by 2-oxoglutarate, but only AOX1A is additionally activated

97 y had been blocked by the deletions and that 2-oxoglutarate can be converted to succinate in vivo in

98 f mitochondrial transport of 2OG through the 2-oxoglutarate carrier (OGC) participates in control of

99 ylnicotinamide, methionine, acetylcarnitine, 2-oxoglutarate, choline, and creatine.

100 lytic iron center is exposed to solvent, the 2-oxoglutarate co-substrate likely adopts an inactive co

101 These enzymes use an Fe(II) cofactor and 2-oxoglutarate co-substrate to oxidize organic substrate

102 ine 8 or 5-carboxy-8-hydroxyquinoline 9, two 2-oxoglutarate competitive templates developed for JmjC

103 NJ-42041935, was a potent (pK(I) = 7.3-7.9), 2-oxoglutarate competitive, reversible, and selective in

104 For the Fe(II) in the DAOCS-2-oxoglutarate complex the EXAFS spectrum was successful

105 ta strand core and residues binding iron and 2-oxoglutarate, consistent with divergent evolution with

106 ess should be amenable to the assay of other 2-oxoglutarate-consuming enzymes and to the discovery of

107 Severe decreases were also seen in 2-oxoglutarate content, a key indicator of cellular carb

108 N269H mutations caused partial uncoupling of 2-oxoglutarate conversion from phytanoyl-CoA oxidation.

109 eir primary substrates while decomposing the 2-oxoglutarate cosubstrate to form succinate and CO(2).

110 uctive interaction occurs with the analogous 2-oxoglutarate decarboxylase (E1o) of the 2-oxoglutarate

111 r1022 and combinations thereof, deficient in 2-oxoglutarate decarboxylase (Sll1981), succinate semial

112 Genes encoding a novel 2-oxoglutarate decarboxylase and succinic semialdehyde d

113 Independent of the 2-oxoglutarate decarboxylase bypass, the gamma-aminobuty

114 The Deltasll1981 strain, lacking 2-oxoglutarate decarboxylase, exhibited a succinate leve

115 olite flux to succinate than the pathway via 2-oxoglutarate decarboxylase.

116 succinyltransferase (Dlst), a subunit of the 2-oxoglutarate dehydrogenase (alpha-KGDH) complex.

117 (a) Functionally competent 2-oxoglutarate dehydrogenase (E1o-h) and dihydrolipoyl s

118 However, the 2-oxoglutarate dehydrogenase (OGDH), branched-chain 2-ox

119 quire the expression of the TCA cycle enzyme 2-oxoglutarate dehydrogenase (OGDH).

120 complete in many other anaerobes (absence of 2-oxoglutarate dehydrogenase activity), isotopic labelin

121 acid as a cofactor (pyruvate dehydrogenase, 2-oxoglutarate dehydrogenase and glycine decarboxylase).

122 e to succinate and thus functionally replace 2-oxoglutarate dehydrogenase and succinyl-CoA synthetase

123 arboxylic acid (TCA) cycle because they lack 2-oxoglutarate dehydrogenase and thus cannot convert 2-o

124 acid dehydrogenase complex (BCOADC), and the 2-oxoglutarate dehydrogenase complex (OGDC).

125 The 2-oxoglutarate dehydrogenase complex (OGHDC) (also known

126 -dependent E1o component (EC 1.2.4.2) of the 2-oxoglutarate dehydrogenase complex catalyses a rate-li

127 dehydrogenase complex E (BCOADC-E2), and the 2-oxoglutarate dehydrogenase complex E (OGDC-E2).

128 -E2) in 6 of 19 patients (31.6%), and to the 2-oxoglutarate dehydrogenase complex E2 (OGDC-E2) in 1 o

129 2 (BCOADC-E2) in 4 of 49 (8%), to PDC-E2 and 2-oxoglutarate dehydrogenase complex E2 (OGDC-E2) in 9 o

130 t of the gene encoding the E1 subunit of the 2-oxoglutarate dehydrogenase complex in the antisense or

131 A traditional 2-oxoglutarate dehydrogenase complex is missing in the c

132 cle enzymes, pyruvate dehydrogenase complex, 2-oxoglutarate dehydrogenase complex, NAD-malic enzyme,

133 2-oxo-acid dehydrogenase, and E2 subunit of 2-oxoglutarate dehydrogenase complex.

134 us 2-oxoglutarate decarboxylase (E1o) of the 2-oxoglutarate dehydrogenase complex.

135 onents of pyruvate dehydrogenase complex and 2-oxoglutarate dehydrogenase complex.

136 We report that the intact pyruvate and 2-oxoglutarate dehydrogenase complexes specifically copu

137 inyl CoA ligase, aconitase, and pyruvate and 2-oxoglutarate dehydrogenase complexes.

138 ith engineered variants of the E2 subunit of 2-oxoglutarate dehydrogenase indicate that binding sites

139 in this organism, even though a traditional 2-oxoglutarate dehydrogenase is lacking.

140 are reported unique properties of the human 2-oxoglutarate dehydrogenase multienzyme complex (OGDHc)

141 bunit binding domain from Escherichia coli's 2-oxoglutarate dehydrogenase multienzyme complex (termed

142 e succinyltransferase (E2o) component of the 2-oxoglutarate dehydrogenase multienzyme complex is comp

143 succinyltransferase polypeptide chain of the 2-oxoglutarate dehydrogenase multienzyme complex of Esch

144 te interactions with other components of the 2-oxoglutarate dehydrogenase multienzyme complex.

145 se (E2p, E2o) components of the pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes are s

146 amine diphosphate-dependent Escherichia coli 2-oxoglutarate dehydrogenase, which is a key component o

147 lding domain of a large multienzyme complex, 2-oxoglutarate dehydrogenase.

148 Pyruvate and 2-oxoglutarate dehydrogenases are substituted by 'ancien

149 Feruloyl-CoA 6'-hydroxylase (F6'H), a 2-oxoglutarate dependent dioxygenase (2OGD), catalyzes a

150 The Fe(II) and 2-oxoglutarate dependent oxygenase Jmjd6 has been shown

151 gamma-Butyrobetaine hydroxylase (BBOX) is a 2-oxoglutarate dependent oxygenase that catalyzes the fi

152 conserved eukaryotic subfamily of Fe(II) and 2-oxoglutarate dependent oxygenases; their catalytic dom

153 and heterologous expression, we identified a 2-oxoglutarate-dependent dioxygenase (BX13) that catalyz

154 The protein level of a probable 2-oxoglutarate-dependent dioxygenase 2-ODD2, involved in

155 The Fe(II) and 2-oxoglutarate-dependent dioxygenase deacetoxycephalospo

156 dioxygenase (HtxA) is a novel member of the 2-oxoglutarate-dependent dioxygenase enzyme family.

157 The TET enzymes are members of the 2-oxoglutarate-dependent dioxygenase family and comprise

158 sis, homozygous mutations in the Fe(II)- and 2-oxoglutarate-dependent dioxygenase family gene F6'H1 a

159 Tpa1 is a member of the Fe(II) and 2-oxoglutarate-dependent dioxygenase family, and we show

160 In a mutant screening, we identified 2-oxoglutarate-dependent dioxygenase Feruloyl-CoA 6'-Hyd

161 -5-hydroxylation catalyzed by the Fe(II) and 2-oxoglutarate-dependent dioxygenase Jumonji domain-6 pr

162 mber of the non-heme-containing iron(II) and 2-oxoglutarate-dependent dioxygenase superfamily and is

163 oxidase cluster of the Arabidopsis thaliana 2-oxoglutarate-dependent dioxygenase superfamily tree.

164 otein (JMJD6) is a JmjC-containing iron- and 2-oxoglutarate-dependent dioxygenase that demethylates h

165 also known as Egl nine homolog 1 (EGLN1), a 2-oxoglutarate-dependent dioxygenase that hydroxylates H

166 he bacterial DNA repair enzyme AlkB, an iron/2-oxoglutarate-dependent dioxygenase that reverses alkyl

167 a coli that DES has the characteristics of a 2-oxoglutarate-dependent dioxygenase.

168 4-hydroxylase isoform 1 (PHD1), an iron and 2-oxoglutarate-dependent dioxygenase.

169 yloxalylglycine, an inhibitor of Fe(II)- and 2-oxoglutarate-dependent dioxygenases also inhibited AhR

170 The Fe(II)/2-oxoglutarate-dependent dioxygenases are a catalyticall

171 Iron- and 2-oxoglutarate-dependent dioxygenases are a diverse fami

172 scription factor alpha subunit by oxygen and 2-oxoglutarate-dependent dioxygenases promotes decay of

173 enerated by a series of non-haem Fe(II)- and 2-oxoglutarate-dependent dioxygenases that catalyse the

174 signal is generated by a series of iron and 2-oxoglutarate-dependent dioxygenases that catalyze post

175 lyl 4-hydroxylases are a family of iron- and 2-oxoglutarate-dependent dioxygenases that negatively re

176 Like other iron/2-oxoglutarate-dependent dioxygenases, AlkB employs a tw

177 The FNSI class comprises soluble Fe(2+)/2-oxoglutarate-dependent dioxygenases, and FNSII enzymes

178 Gibberellin (GA) 3-oxidase, a class of 2-oxoglutarate-dependent dioxygenases, catalyzes the con

179 ncometabolites and competitive inhibition of 2-oxoglutarate-dependent dioxygenases, particularly, hyp

180 low amino acid sequence homology with known 2-oxoglutarate-dependent dioxygenases, putative iron- an

181 e kinetic parameters of two bacterial Fe(II)/2-oxoglutarate-dependent dioxygenases.

182 , it will be a valuable tool to study Fe(II)/2-oxoglutarate-dependent dioxygenases.

183 These levels of R-2-hydroxyglutarate affect 2-oxoglutarate-dependent dioxygenases.

184 The TET family of FE(II) and 2-oxoglutarate-dependent enzymes (Tet1/2/3) promote DNA

185 is generated by the TET family of Fe(II) and 2-oxoglutarate-dependent enzymes through oxidation of 5-

186 tructural characteristics of non-heme Fe(II) 2-oxoglutarate-dependent enzymes, although key enzymatic

187 substantiated by the pioneering discovery of 2-oxoglutarate-dependent flavone demethylase activity in

188 tumors accumulate succinate, which inhibits 2-oxoglutarate-dependent histone and DNA demethylase enz

189 directly decreased the activity of a Fe(II)-2-oxoglutarate-dependent histone H3K9 demethylase in nuc

190 ve TH domain related to the family of Fe(2+)/2-oxoglutarate-dependent hydroxylases.

191 JMJD6 catalyses the iron- and 2-oxoglutarate-dependent hydroxylation of lysyl residues

192 The 2-oxoglutarate-dependent iron enzyme ALKBH3 is an antitu

193 eport that recombinant PHF8 is an Fe(II) and 2-oxoglutarate-dependent N(epsilon)-methyl lysine demeth

194 2-Oxoglutarate-dependent nucleic acid demethylases are o

195 in C synthase (DAOC/DACS) is an iron(II) and 2-oxoglutarate-dependent oxygenase involved in the biosy

196 ystallographic data for other members of the 2-oxoglutarate-dependent oxygenase super-family led to s

197 sporin C synthase (DAOCS) is an iron(II) and 2-oxoglutarate-dependent oxygenase that catalyzes the co

198 A widely used generic assay for 2-oxoglutarate-dependent oxygenases relies upon monitori

199 of the HIF system is provided by Fe(II) and 2-oxoglutarate-dependent oxygenases that catalyse the po

200 us iron binding residues that are present in 2-oxoglutarate-dependent oxygenases.

201 FTO shares sequence motifs with Fe(II)- and 2-oxoglutarate-dependent oxygenases.

202 d cocontrolled by PHD2 and PHD3, oxygen- and 2-oxoglutarate-dependent prolyl-4-hydroxylases that regu

203 ated by using wild-type and variant forms of 2-oxoglutarate-dependent taurine dioxygenase.

204 2-Oxoglutarate-dependent versions appear to have further

205 vely, Grob-type oxidative fragmentation of a 2-oxoglutarate-derived intermediate occurs to give ethyl

206 2-Oxoglutarate did not affect activity in DeltanifI(1)ni

207 2-Oxoglutarate diminishes AmtB/GlnK association, and sit

208 uence comparisons suggest that hypophosphite:2-oxoglutarate dioxygenase (HtxA) is a novel member of t

209 BCDEFGHIJKLMN operon encodes a hypophosphite-2-oxoglutarate dioxygenase (HtxA), whereas the predicted

210 Whereas the Fe-2-oxoglutarate dioxygenase core matches that in other su

211 f vitamin C, a potential cofactor for Fe(II) 2-oxoglutarate dioxygenase enzymes such as Tet enzymes.

212 ochondrial poison cyanide or the nonspecific 2-oxoglutarate dioxygenase inhibitor dimethyloxalylglyci

213 nder the tested conditions, a broad-spectrum 2-oxoglutarate dioxygenase inhibitor is a better mimic o

214 Ofd1, a prolyl 4-hydroxylase-like 2-oxoglutarate dioxygenase, controls the oxygen-dependen

215 ation of ATF3 under anoxia is independent of 2-oxoglutarate dioxygenase, HIF-1 and p53, presumably in

216 s also striking enrichment for the family of 2-oxoglutarate dioxygenases, including the jumonji-domai

217 an uncharacterized prolyl 4-hydroxylase-like 2-oxoglutarate-Fe(II) dioxygenase, accelerates Sre1N deg

218 These findings further highlight the role of 2-oxoglutarate/Fe(II) oxygenases in fundamental cellular

219 n morphine biosynthesis are catalyzed by the 2-oxoglutarate/Fe(II)-dependent dioxygenases, thebaine 6

220 DhpJ, a 2-oxoglutarate/Fe(II)-dependent enzyme, introduces the v

221 report the identification of four paralogous 2-oxoglutarate/Fe(II)-dependent oxygenases in Arabidopsi

222 tricarboxylic acid cycle, ATP citrate lyase, 2-oxoglutarate:ferredoxin oxidoreductase, and pyruvate:f

223 phosphite in a process strictly dependent on 2-oxoglutarate, ferrous ions, and oxygen.

224 no acid directly competes with the substrate 2-oxoglutarate for binding within the active site of HCS

225 iological function of NADH-GDH is to provide 2-oxoglutarate for the tricarboxylic acid cycle.

226 anti-correlation between 2-hydroxyglutarate, 2-oxoglutarate, fructose, hexadecanoic acid, hypotaurine

227 s a two-step mechanism in which oxidation of 2-oxoglutarate generates a highly reactive enzyme-bound

228 artate and reintroduced in the TCA cycle via 2-oxoglutarate/glutamate.

229 nal the carbon status through the binding of 2-oxoglutarate, have been implicated in the regulation o

230 rginine in a nonoxidized conformation and of 2-oxoglutarate in an unprecedented high-energy conformat

231 organisms includes demonstrating the role of 2-oxoglutarate in regulating the activity of the transcr

232 The P(II) effector 2-oxoglutarate, in the presence of Mg-ATP, inhibited Dra

233 Extraction and quantification of 2-oxoglutarate indicated concentrations 10-fold higher i

234 ln, glutamate, and the anaplerotic substrate 2-oxoglutarate, inhibiting MM cell growth.

235 To determine pathways that convert 2-oxoglutarate into succinate in the cyanobacterium Syne

236 t an alternative assay in which depletion of 2-oxoglutarate is monitored by its postincubation deriva

237 The iron and 2-oxoglutarate ligands are bound within the EctD active

238 , pyruvate, orthophosphate dikinase, and the 2'-oxoglutarate/malate transporter are expressed in oat

239 ell as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytan

240 eveal that 2-hydroxyglutarate is oxidized to 2-oxoglutarate non-enzymatically, likely via iron-mediat

241 Deficiency in 2-oxoglutarate occurred despite increased citrate and ma

242 in resonance upon reaction of the E1o-h with 2-oxoglutarate (OG) by itself or when assembled from ind

243 However, the dicarboxylate (DIC) and 2-oxoglutarate (OGC) carriers localized to the inner mit

244 ntial for function of the pyruvate (PDH) and 2-oxoglutarate (OGDH) dehydrogenases and thus for aerobi

245 ability to override the allosteric effect of 2-oxoglutarate on NifA activity.

246 eine, histidine, and ferrous iron but not by 2-oxoglutarate or oxygen.

247 should not solely rely upon PAHX assays for 2-oxoglutarate or phytanoyl-CoA oxidation.

248 hondria also inhibited State 3 succinate and 2-oxoglutarate oxidation by 30 %, but not that of palmit

249 tion rather than activation of succinate and 2-oxoglutarate oxidation.

250 In one branch, an apparently typical 2-oxoglutarate oxygenase reaction to give succinate, car

251 of 2-hydroxyglutarate-enabled activation of 2-oxoglutarate oxygenases, including prolyl hydroxylase

252 , at levels supporting in vitro catalysis by 2-oxoglutarate oxygenases.

253 olic flux using (13)C labelling; acetate and 2-oxoglutarate production was reduced in the light.

254 e stimulated and inhibited, respectively, by 2-oxoglutarate, providing a mechanistic link between PII

255 tilizing the typical keto-acid cosubstrates, 2-oxoglutarate, pyruvate, and oxaloacetate, Ab-ArAT4 pos

256 a variety of nitrogen assimilation genes by 2-oxoglutarate-reversible binding to conserved palindrom

257 Pyruvate and oxaloacetate bind to the 2-oxoglutarate site of HIF-1alpha prolyl hydroxylases, b

258 id cycle intermediates (citrate, isocitrate, 2-oxoglutarate, succinate, fumarate, malate, and oxaloac

259 (3) We need to determine whether the 2-oxoglutarate synthase (ferredoxin-dependent) (EC 1.2.7

260 air yields slow but substantial oxidation of 2-oxoglutarate that is inefficiently coupled to nucleoti

261 iring for activity both molecular oxygen and 2-oxoglutarate that, under normoxia, selectively hydroxy

262 of a novel enzymatic activity that converts 2-oxoglutarate to D-2-hydroxyglutarate.

263 in fungi by condensing acetyl-coenzyme A and 2-oxoglutarate to form 3R-homocitrate and coenzyme A.

264 eversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate, and there

265 tion, but via the four-electron oxidation of 2-oxoglutarate to give ethylene in an arginine-dependent

266 Pyl is also able to ligate 2-oxoglutarate to other 4-amino-sugar derivatives to for

267 Together, these two enzymes convert 2-oxoglutarate to succinate and thus functionally replac

268 tarate dehydrogenase and thus cannot convert 2-oxoglutarate to succinyl-coenzyme A (CoA).

269 hat, in the presence of ATP and Mg(II), adds 2-oxoglutarate to the 4-amino moiety of UDP-4-amino-FucN

270 rates that this mutation prevents binding of 2-oxoglutarate to the GAF domain.

271 Addition of 2-oxoglutarate to the growth media of the double mutant

272 Addition of 2-oxoglutarate to wild-type extracts enhanced activity u

273 DeltaR306 mutant complexed with iron(II) and 2-oxoglutarate (to 2.10 A) and the DeltaR306A mutant com

274 ansamination enzymes, namely 4-aminobutyrate-2-oxoglutarate transaminase (GABA-T) and alanine-glyoxyl

275 alian transaminating enzymes 4-aminobutyrate-2-oxoglutarate transaminase and alanine-glyoxylate trans

276 mature quenching by solvent, which uncouples 2-oxoglutarate turnover from nucleobase oxidation.

277 lved in the synthesis of UDP-FucNAc-4-amido-(2)-oxoglutarate (UDP-Yelosamine), a modified UDP-sugar n

278 ole in counteracting the response of NifA to 2-oxoglutarate, under conditions that are inappropriate

279 ible oxidative deamination of l-glutamate to 2-oxoglutarate using NAD(P)(+) as coenzyme.

280 DAOCS was crystallized +/-Fe(II) and/or 2-oxoglutarate using the hanging drop method.

281 Oxidation of 2-oxoglutarate was significantly uncoupled from oxidatio

282 e apparent K(m) values for hypophosphite and 2-oxoglutarate were 0.58 +/- 0.04 mm and 10.6 +/- 1.4 mi

283 121.2 and 7121.4 eV for DAOCS alone and with 2-oxoglutarate were both consistent with the presence of

284 ty of NIFL is relieved by elevated levels of 2-oxoglutarate when PII is uridylylated under conditions

285 ivity that is counteracted by high levels of 2-oxoglutarate, which acts as a signal of nitrogen limit

286 deamination activity of GDH might regenerate 2-oxoglutarate, which is a cosubstrate that facilitates

287 e oxidative decarboxylation of isocitrate to 2-oxoglutarate with a specific activity of 22.5 units/mg

288 es the oxidative deamination of glutamate to 2-oxoglutarate with concomitant reduction of NAD(P)(+),

289 e-N at 1.99 A), a bidentate O,O-co-ordinated 2-oxoglutarate with Fe-O distances of 2.08 A, another O