ライフサイエンスコーパス: 3' splice site

1 hibiting splicing at that exon's alternative 3' splice site.

2 g components that recognize either the 5' or 3' splice site.

3 re than 13 ribonucleotides downstream of the 3' splice site.

4 tion of nucleotide 'C' at position -3 of the 3' splice site.

5 idine (Py) tract consensus sequence near the 3' splice site.

6 thout formation of a splicing complex with a 3' splice site.

7 ate the recognition of weak Py-tracts at the 3' splice site.

8 proteins through activation of the optimized 3' splice site.

9 to authentic substrates that lack a U12-type 3' splice site.

10 aring AT at the 5' splice site and AC at the 3' splice site.

11 ated at the 5' splice site, branch site, and 3' splice site.

12 S, ESSV, which regulates splicing at the vpr 3' splice site.

13 be synthesized as much as an hour before the 3' splice site.

14 efficiency caused by the intron's suboptimal 3' splice site.

15 th a preference for 70-80 nt upstream of the 3' splice site.

16 prespliceosomal complex A requires an active 3' splice site.

17 r shorter spacers between the snoRNA and the 3' splice site.

18 me appears to be unable to scan for a distal 3' splice site.

19 a sequence that highly resembles a bona fide 3' splice site.

20 n the presence of a suboptimal non-consensus 3' splice site.

21 n by directly interacting with the AG at the 3' splice site.

22 d at the polypyrimidine tract of the default 3' splice site.

23 ions with the 2'- and 3'-oxygen atoms at the 3' splice site.

24 cing of a pre-mRNA with an optimized nt 3225 3' splice site.

25 SE4 also functions on a heterologous 3' splice site.

26 onically located with respect to the nt 3605 3' splice site.

27 2 is spliced predominantly using the nt 3605 3' splice site.

28 ly conserved, and closely distributed to the 3' splice site.

29 nals: a 5' splice site, a branch site, and a 3' splice site.

30 ex, which assembles across the intron at the 3' splice site.

31 an ATP-dependent process requiring an intact 3' splice site.

32 e 5' splice site and the terminator with the 3' splice site.

33 (65) recognize a polypyrimidine tract at the 3' splice site.

34 or with critical roles in recognition of the 3'-splice site.

35 ng a potential cis-regulatory element at the 3'-splice site.

36 by mutations and increased distance from the 3'-splice site.

37 e of binding of the guanosine specifying the 3'-splice site.

38 m A(C)' to A(C) appears to require an intact 3'-splice site.

39 cleotides from the 3'-splice site and an AAG 3'-splice site.

40 ition of exons that are flanked by the HIV-1 3' splice sites.

41 ize polypyrimidine tract signals adjacent to 3' splice sites.

42 lice site that interacts with and sequesters 3' splice sites.

43 tly inhibiting use of intron-proximal 5' and 3' splice sites.

44 by point mutations that improve their 5' or 3' splice sites.

45 of the U1/U11 site that is characteristic of 3' splice sites.

46 form long-range interactions with the 5' and 3' splice sites.

47 combinations were optimal for distinguishing 3' splice sites.

48 65 to the polypyrimidine tracts of repressed 3' splice sites.

49 for and utilize alternative branch sites and 3' splice sites.

50 0 nucleotides strongly activated alternative 3' splice sites.

51 hile allowing flexibility in the location of 3' splice sites.

52 by enhancing the binding of U2AF(65) to weak 3' splice sites.

53 ent for SF1/mBBP-U2AF complexes assembled at 3' splice sites.

54 occurred exclusively at the original 5' and 3' splice sites.

55 undreds of introns with adjacent alternative 3' splice sites.

56 alternative 5' splice sites, and alternative 3' splice sites.

57 rly spliceosome components bound proximal to 3' splice sites.

58 sensus sequence preceding the major class of 3' splice sites.

59 iral late gene transcripts that contain weak 3' splice sites.

60 ing (AS) by differential selection of 5' and 3' splice sites.

61 p chemistry of precursors with inappropriate 3'-splice sites.

62 precedented RNA motifs to select the 5'- and 3'-splice sites.

63 uired for the initial recognition of 5'- and 3'-splice sites.

64 on similarly in activating regulated 5'- and 3'-splice sites.

65 ation of Intron-Exon and Exon-Intron (5' and 3') splice sites.

66 In the case of SR45, the use of alternative 3' splice sites 21 nucleotides apart generates two alter

67 ong branch site (BS), a long distance to the 3' splice site (3' SS), and a weak polypyrimidine (Py) t

68 ctions between the 5' splice site (5'ss) and 3' splice site (3'ss) were observed in human/mouse, indi

69 pproximately 1 kb after transcription of the 3' splice site (3'SS).

70 dine tract (PPT) and AG dinucleotides at the 3' splice site (3'ss).

71 ains three 5' splice sites (5' ss) and three 3' splice sites (3' ss) normally used in HPV16(+) cervic

72 ites; (iii) divergent evolution of C.elegans 3' splice sites (3'ss) and (iv) distinct evolutionary hi

73 cy distribution of mutation-induced aberrant 3' splice sites (3'ss) in exons and introns is more comp

74 Regulation of splicing at the HIV-1 3' splice sites (3'ss) requires suboptimal polypyrimidin

75 Regulation of splicing at HIV-1 3' splice sites (3'ss) requires suboptimal polypyrimidin

76 d sequences of previously published aberrant 3' splice sites (3'ss) that were generated by mutations

77 s, pyrimidine-rich regions [poly(Y) tracts], 3' splice sites (3'SS), and sometimes enhancer elements.

78 or samples, we show that hundreds of cryptic 3' splice sites (3'SSs) are used in cancers with SF3B1 m

79 ith aberrant pre-mRNA splicing using cryptic 3' splice sites (3'SSs), but the mechanism of their sele

80 promoted by cytosine at rs609621 in the NSE 3' splice-site (3'ss), which is predominant in high canc

81 otein interactions involving the branch site-3' splice site-3' exon region during yeast pre-mRNA spli

82 associates extensively with the branch site-3' splice site-3' exon region.

83 The selection of 3 splice sites (3ss) is an essential early step in mamma

84 n definition" mechanism, in which the 5' and 3' splice sites (5'ss, 3'ss, respectively) are initially

85 e we show that the efficiency of splicing at 3' splice site A2, which is used to generate Vpr mRNA, i

86 endent introns containing alterations of the 3' splice site AC dinucleotide or alterations in the spa

87 Five 5' splice sites (donor sites) and six 3' splice sites (acceptor sites) that are highly conserv

88 The pairing of 5' and 3' splice sites across an intron is a critical step in s

89 Our results provide a mechanism for exon 16 3' splice site activation in which a coordinated effort

90 When the 3' splice site AG is supplied by a separate RNA, that RN

91 The 3'-splice site AG dinucleotide is recognized through non

92 retains the IGS extension, and with 5'- and 3'-splice site analogues that differ in their ability to

93 TMyoD1-beta arises from an alternative 3' splice site and differs from TMyoD1-alpha by a 26-res

94 In TRIM62, the upstream 3' splice site and downstream intronic poly-G runs funct

95 RNA expression and splicing at the proximal 3' splice site and enhanced Akt phosphorylation and expr

96 loosely associated tri-snRNP, sequesters the 3' splice site and prevents its interaction with the aut

97 e 5' splice site, components recognizing the 3' splice site and proteins thought to connect them.

98 e protein that has a role in recognizing the 3' splice site and recruiting U2 snRNP.

99 ssential splicing factor that recognizes the 3' splice site and recruits the U2 snRNP to the branch p

100 investigated the late-stage-specific nt 3605 3' splice site and showed that it has suboptimal feature

101 ntron can inhibit splicing to the downstream 3' splice site and that this inhibition is independent o

102 the lariat intermediate is restricted to the 3' splice site and the adjacent 3' exon sequence.

103 Communication between the 3' splice site and the poly(A) site across the terminal

104 ce site polymorphisms, most notably a strong 3' splice site and the presence of intronic motifs downs

105 ) binding sites, are located between the two 3' splice sites and have been identified as regulating a

106 otide branch point sequence (BPS) located at 3' splice sites and participates in the assembly of earl

107 isitely sensitive to the sequence context of 3' splice sites and to small structural differences betw

108 ranch point that is 141 nucleotides from the 3'-splice site and an AAG 3'-splice site.

109 ringing the 5'-splice site together with the 3'-splice site and catalytic core elements at JII/III.

110 together to identify the 5 splice site, the 3 splice site, and the branchsite (BS) of nascent pre-mR

111 be alternatively recognized as either 5' or 3' splice sites, and the dual splicing is conceptually s

112 in CA-RNA are within 50 nucleotides of 5' or 3' splice sites, and the vast majority of exons harborin

113 and the entire 3' exon, including the mutant 3' splice site, are accessible and can be removed by nuc

114 Once the 3' splice site at ancient Alu-exons reaches a stable pha

115 ion of the branch site, pyrimidine tract and 3' splice site at the 3' end of introns.

116 we propose a role for Cwc21p positioning the 3' splice site at the transition to the second step conf

117 5' splice sites at nt 232 and nt 898 and two 3' splice sites at nt 510 and nt 3355 can be identified.

118 These chimeras contain discernable 5' and 3' splice sites at the RNA junction, indicating that the

119 ice site alternatively splices to a proximal 3' splice site (at nucleotide 3225) to express L2 or to

120 ucleotide 3225) to express L2 or to a distal 3' splice site (at nucleotide 3605) to express L1.

121 ngle nucleotide polymorphism that alters the 3' splice site between intron 6 and exon 7.

122 te is recognized in the absence of an active 3' splice site but that formation of the prespliceosomal

123 RNA expression and splicing at the proximal 3' splice site, but activation-rescued viral RNA express

124 lly present between the branch point and the 3' splice site by the large subunit of the essential spl

125 nition of regulatory RNA cis elements in the 3' splice site by the U2 auxiliary factor (U2AF).

126 s splicing at several highly conserved HIV-1 3' splice sites by binding 5'-UAG-3' elements embedded w

127 The relative use of a dual site as a 5' or 3' splice site can be accurately predicted by assuming c

128 regulatory elements in 4.1R pre-mRNA govern 3' splice site choice at exon 2 (E2) via nested splicing

129 er with ELAV, is required to regulate neural 3' splice site choice in vivo.

130 those that regulate alternative sex-specific 3'-splice site choice in the doublesex (dsx) gene.

131 For E2, alternative 3'-splice site choice is coordinated with upstream promo

132 stigate the mechanisms of enhancer-dependent 3'-splice site choice.

133 shift toward usage of the adjacent proximal 3' splice site (closer to the 5' end of the intron).

134 of Neurospora introns are much closer to the 3' splice site compared to those in human introns.

135 ylation in proviral clones only when the NRS-3' splice site complex could form.

136 SV polyadenylation in the context of the NRS-3' splice site complex, which is thought to bridge the l

137 th SF3A1 mediates contact between the 5' and 3' splice site complexes within the assembling spliceoso

138 essentially a composite of canonical 5' and 3' splice-site consensus sequences, with a CAG|GURAG cor

139 Tb(3+) cleavage was redirected to the 5' and 3' splice sites, consistent with metal-dependent activat

140 reaction involves the association of 5' and 3' splice sites contained on separate transcripts.

141 modify its own pre-mRNA to create a proximal 3' splice site containing a noncanonical adenosine-inosi

142 c cells follow C. elegans consensus rules of 3' splice site definition; a short stretch of pyrimidine

143 es, suggesting SFPS might be involved in the 3' splice site determination.

144 Removal of the cryptic Neo 3' splice site did not reduce the proportion of clones w

145 removal of introns with short branchpoint-to-3' splice site distances.

146 e and stabilize a conformation competent for 3'-splice site docking, thereby promoting exon ligation.

147 actor (U2AF(65)) cooperatively recognize the 3' splice site during the initial stages of pre-mRNA spl

148 ng the AG dinucleotide that functions as the 3' splice site during the second transesterification ste

149 iously unrecognized role in the selection of 3' splice sites during the second step of splicing.

150 ZRSR2 is involved in the recognition of 3'-splice site during the early stages of spliceosome as

151 First, the 3' splice site establishes connections to enhance 3'-end

152 t exons splice differentially to alternative 3' splice sites far downstream in exon 2'/2 (E2'/2).

153 late RNAs and for selection of the proximal 3' splice site for BPV-1 RNA splicing in DT40-ASF cells,

154 tment of several RNA splicing factors to the 3' splicing site for AR-V7 was increased.

155 wo binding regions, each ending with a known 3' splice site, for U2AF(35) b.

156 s distinguishes the guanosine at the correct 3'-splice site from other guanosine residues, the faster

157 te A residue, by contrast, was essential for 3' splice site function.

158 tic cells preferring to splice at the distal 3' splice site (furthest from the 5' end of the intron)

159 sites and the RNA structure near the 5' and 3' splice sites has fueled speculation that such protein

160 lementary mechanisms of U2AF recruitment and 3' splice site identification exist to accommodate diver

161 Bioinformatic analysis revealed that the 3' splice sites identified in three of these putative IR

162 ginine (SR)-rich proteins activate a cryptic 3' splice site in a sense Alu repeat located in intron 4

163 Deletions of the 3' splice site in intron 2 or in both introns 1 and 2 of

164 We found that the use of an alternative 3' splice site in intron 6 generates a unique p53 isofor

165 ted genes that spliced in-frame to a cryptic 3' splice site in the Neo coding sequence and expressed

166 lice site while increasing use of the distal 3' splice site in the remaining viral RNAs.

167 165 b (resulting from alternative usage of a 3' splice site in the terminal exon) is protective for k

168 3' splice site mutation, can accept a normal 3' splice site in trans to catalyze exon ligation.

169 e ESS inhibits use of the suboptimal nt 3225 3' splice site in vitro through binding of cellular spli

170 electively increase splicing at the upstream 3' splice sites in cotransfected 293T cells.

171 yrimidine (Py) signals of the major class of 3' splice sites in human gene transcripts remains incomp

172 ranchsites, polypyrimidine tracts and 5' and 3' splice sites in the intron databases and exonic splic

173 tes recognition of exons with unusual C-rich 3' splice sites in thousands of essential genes.

174 rol intrasplicing at a subset of alternative 3' splice sites in vertebrate pre-mRNAs to generate prot

175 0 pre-mRNA including the UG repeats near the 3'-splice site in the first intron.

176 step of self-splicing, and P10 forms at the 3'-splice site in the second step of self-splicing.

177 and a guanosine in the omega position at the 3' splice site (in the substrate).

178 site eliminated the requirement for the NRS-3' splice site interaction.

179 he normal splicing pattern where the nt 3225 3' splice site is already used predominantly.

180 ine tract between the branch point A and the 3' splice site is associated with increased exon skippin

181 urther studies demonstrated that the nt 3605 3' splice site is controlled by a novel exonic bipartite

182 if situated between the branch point and the 3' splice site is crucial for inclusion.

183 In most eukaryotic introns the 3' splice site is defined by a surprisingly short AG con

184 The polypyrimidine tract near the 3' splice site is important for pre-mRNA splicing.

185 stest introns are gone nearly as soon as the 3' splice site is transcribed and that introns have dist

186 dence that use of germline-specific proximal 3' splice sites is conserved across Caenorhabditis speci

187 We show that evolutionary progression of 3' splice sites is coupled with longer repressive uridin

188 onserved region between the branch point and 3'-splice site is primarily unstructured and that MBNL1

189 uence arrangements exist, however, including 3' splice sites lacking recognizable Py tracts, which ra

190 f a second CU-rich upstream of the mini-exon 3' splice site led to a decline in mini-exon splicing, i

191 FV pol splicing uses a 3' splice site located at the 3' end of gag, resulting i

192 u proteins also decrease U2AF binding at the 3' splice site located upstream of exon 23a.

193 how that U2AF1 mutations alter the preferred 3' splice site motif in patients, in cell culture, and i

194 inalis introns have a highly conserved 12-nt 3' splice-site motif that encompasses the branch point a

195 n conformation adopted late in splicing by a 3' splice-site mutant, invoking a mechanism for substrat

196 site of the complex, which is arrested by a 3' splice site mutation, can accept a normal 3' splice s

197 uding 16 insertion/deletion, 3 nonsense, and 3 splice-site mutations.

198 xon ligation, disrupting stem IIa suppressed 3' splice site mutations, and disrupting stem IIc impair

199 tion of exon skipping and tandem alternative 3' splice sites (NAGNAGs) were more divergent than other

200 wnstream of the vpu-, env-, and nef-specific 3' splice site no. 5.

201 phenotype of the A-to-G substitution in the 3' splice site of BBS8 exon 2A (IVS1-2A>G mutation) in t

202 ntisense oligonucleotide (AON) targeting the 3' splice site of ClC-1 exon 7a reversed the defect of C

203 Additionally, the 3' splice site of E10 is weak and requires exon splicing

204 single-nucleotide polymorphism (SNP) at the 3' splice site of exon 10 of the human P2X5 gene.

205 l mutation is a single point mutation in the 3' splice site of exon 4 leading to an exon extension an

206 assembly of the splicing factor U2AF on the 3' splice site of exon 4.

207 erence reduces the intrinsic strength of the 3' splice site of exon 7 2-fold, whereas the strength of

208 Gsdm3(Dfl) is a B2 insertion near the 3' splice site of exon 7 and Gsdm3(Fgn) is a point mutat

209 1 and interfering with that of U2AF65 at the 3' splice site of exon 7.

210 Oligonucleotides directed toward the 3' splice site of exon 8 were shown to alter SMN2 splici

211 pends on two UGCAUG elements surrounding the 3' splice site of the calcitonin-specific exon 4.

212 laps with the polypyrimidine tract of the A2 3' splice site of the central intron.

213 ptimal position about 70 nts upstream of the 3' splice site of the host intron is critical for effici

214 ose few box C/D snoRNAs located far from the 3' splice site of their host intron.

215 lated polymerase also accumulates around the 3' splice sites of constitutively expressed, endogenous

216 The AG dinucleotide at the 3' splice sites of metazoan nuclear pre-mRNAs plays a cr

217 the active sites for cleavage at the 5' and 3' splice sites of precursor tRNA are contained within S

218 The 5' and 3' splice sites of the intron of the spliced RNA are wit

219 Splicing factor 1 (SF1) recognizes 3' splice sites of the major class of introns as a terna

220 exon splicing with Mbnl binding near either 3' splice site or near the downstream 5' splice site, re

221 , but does not require the branch point, the 3' splice site, or the 5' end of the U1 snRNA.

222 tion involving the use of a non-canonical TG 3'-splice site preceding exon 4.

223 cellular transcripts that spliced to cryptic 3' splice sites present either within the targeting vect

224 her, the fusion transcripts utilized cryptic 3' splice sites present in the adjacent intron or genera

225 ted exon inclusion, whereas binding near the 3' splice site promoted either exon skipping or inclusio

226 s to identify important functional groups in 3' splice site recognition and catalysis, we establish h

227 on inclusion in neural cells while weakening 3' splice site recognition and contributing to exon skip

228 ylated SF1 loop are required for cooperative 3' splice site recognition by the SF1-U2AF(65) complex (

229 rmational selection as a means for universal 3' splice site recognition by U2AF(65).

230 gate the molecular mechanism and dynamics of 3' splice site recognition by U2AF65 and the role of U2A

231 ght to determine how mutations affecting the 3' splice site recognition factor U2AF1 alter its normal

232 SKIP interacts with the 3' splice site recognition factor U2AF65 and recruits it

233 rformed in fission yeast support a model for 3' splice site recognition in which the two subunits of

234 simultaneously, thereby potentially linking 3' splice site recognition with tri-snRNP addition.

235 he small subunit of U2AF, which functions in 3' splice site recognition, is more highly conserved tha

236 s is not predicted by the model for metazoan 3' splice site recognition, we sought introns for which

237 The mechanism of 3'-splice site recognition during exon ligation has rema

238 ssociations of proteins with the branch site-3' splice site region during spliceosome assembly and ca

239 in the frequency of three base pair gaps at 3' splice sites relative to nearby exon positions in bot

240 hat SR45 recruits U1snRNP and U2AF to 5' and 3' splice sites, respectively, by interacting with pre-m

241 components involved in recognition of 5' and 3' splice sites, respectively.

242 Our microarray analysis shows changes in 3' splice site selection at elevated temperature in a su

243 shown recently to alter branch site (BS) or 3' splice site selection in splicing.

244 ing by three amino acids through alternative 3' splice site selection in the first intron.

245 Cooperative repression and alternative 3' splice site selection were found to be used by Rbm20

246 icing factor U2AF plays an important role in 3' splice site selection, but the division of labor betw

247 F(35) b that are known to function in 5' and 3' splice site selection, respectively.

248 ties to step II splicing factors involved in 3' splice site selection.

249 nd crossregulate at the level of alternative 3' splice site selection.

250 acceptor site may have an important role in 3' splice site selection.

251 ant MDS patient samples demonstrate aberrant 3' splice-site selection associated with increased nonse

252 ence that SF2/ASF and hnRNPA1 play a role in 3'-splice site selection involving the use of a non-cano

253 y1 PRP16 strains display reduced fidelity of 3'-splice site selection.

254 correct guanosine can enhance specificity of 3'-splice site selection.

255 Insertion of the viral env 3' splice site sequence between the NRS and the LTR did

256 with the widespread occurrence of potential 3' splice site sequences in the introns of cellular gene

257 A branch site-to-3' splice site spacing of less than 10 or more than 20 n

258 uire the snRNP-binding sites or a downstream 3' splice site, SR proteins were sufficient to stimulate

259 Among them, alternative 3 ': splice site (SS) and 5 ': SS account for more than

260 when the inserted test sequence contained a 3' splice site (ss).

261 first exons (1A, 1B, 1C) to the alternative 3' splice sites (ss) of exon 2'/2.

262 lso seen: Effective enhancers, silencers and 3' splice sites tend to be single stranded, and effectiv

263 The cis-acting signals within the A2 3' splice site that govern polyadenylation and splicing

264 Although MVMi contains a 3' splice site that performs poorly in fibroblasts, MVMi

265 plicing in germline cells occurs at proximal 3' splice sites that lack a preceding polypyrimidine tra

266 Adjacent alternative 3' splice sites, those separated by </=18 nucleotides, p

267 ine tract (Py-tract) located adjacent to the 3' splice site to facilitate U2 snRNP recruitment.

268 er, changing a single nucleotide in the MVMi 3' splice site to that found in the fibrotropic strain M

269 eneracy of the genetic code allows competing 3' splice sites to be eliminated from coding regions, an

270 erminants of the differential sensitivity of 3' splice sites to these drugs.

271 Weakening of the 3' splice site uncovered distinct differences, however,

272 splicing by blocking U2AF(65) binding to the 3' splice site upstream of exon 4.

273 Transferring 54 nucleotides from the 3' splice site upstream of STREX to a heterologous gene

274 rrant 5' splice site and activates a cryptic 3' splice site upstream.

275 ivate the ESE and are required for efficient 3' splice site usage and binding of the U1 snRNP to the

276 ve been identified as regulating alternative 3' splice site usage.

277 itively influence utilization of an upstream 3' splice site via exon definition in both trans- and ci

278 g, while substitution with an unrelated weak 3' splice site was compatible with repression, implying

279 plice sites, the proximity to the downstream 3' splice site was more influential in dictating splice

280 ments showed that utilization of the nt 3605 3' splice site was not affected by SE2, which is introni

281 the spacing between the branch site and the 3' splice site were examined for their effects on splice

282 etics of splicing in vitro demonstrated that 3' splice sites were chosen competitively during the sec

283 esistance gene (Neo), a poly(A) site, but no 3' splice site] were typically expressed following inser

284 ntified were located in a region upstream of 3'-splice sites where BPSs are typically found.

285 with one 5' splice site and two alternative 3' splice sites, which produce E6(*)I and E6(*)II, respe

286 expression and RNA splicing at the proximal 3' splice site while increasing use of the distal 3' spl

287 rgeting polypyrimidine (Py) tracts preceding 3' splice sites while adapting to both cytidine and urid

288 d cellular IRESs, we found that four contain 3' splice sites whose activity was required for apparent

289 Substitution of this 3' splice site with a strong splice site resulted in TRA

290 osal that the human large subunit recognizes 3' splice sites with extensive polypyrimidine tracts ind

291 f the BPS, PPT, and AG dinucleotide found at 3' splice sites, with endogenous proteins assembled alon

292 that carried point mutations at or near the 3' splice site within the intergenic region separating C

293 The 5' and 3' splice sites within an intron can, in principle, be j

294 Two of the mutations activated cryptic 5' or 3' splice sites within exonic regions; the third mutatio

295 ative splicing event that utilizes different 3' splice sites within intron 3.

296 experiments, we found a number of functional 3' splice sites within many different transcribed SVAs a

297 17.5, and IE9 transcripts all used different 3'-splice sites within exon 4.

298 nd have short polypyrimidine tracts in their 3' splice sites, yet they are not skipped.

299 he second step, when the 5' exon attacks the 3' splice site, yielding mRNA and lariat-intron products

300 nucleotide), where both NAGs can function as 3' splice sites, yielding isoforms that differ by inclus