ライフサイエンスコーパス: 5' splice site

1 resence of intronic motifs downstream of the 5' splice site.

2 ence forms a hairpin that weakly tethers the 5' splice site.

3 de polymorphisms (SNPs) within the consensus 5' splice site.

4 he initial recruitment of U1snRNP to the PLP 5' splice site.

5 he U6 snRNP at the precursor mRNA (pre-mRNA) 5' splice site.

6 ed in the presence of the competing upstream 5' splice site.

7 splice site located downstream of a weakened 5' splice site.

8 reposition the substrate after cleaving the 5' splice site.

9 ide to induce the activation of the Bcl-x(s) 5' splice site.

10 urther induce the activation of the Bcl-x(s) 5' splice site.

11 wo splicing signals: the branchpoint and the 5' splice site.

12 serves as the nucleophile for attack on the 5' splice site.

13 ot interfere with U1 snRNP binding to the N1 5' splice site.

14 site sequence converted it into a functional 5' splice site.

15 tion of U1 small nuclear RNA with the intron 5' splice site.

16 ecruitment of the splicing complex to a weak 5' splice site.

17 ted 32 nucleotides downstream of the U2-type 5' splice site.

18 he first natural example of such a composite 5' splice site.

19 inding of U1-70K (snRNP70) to the downstream 5' splice site.

20 exons that fail to reconstitute an efficient 5' splice site.

21 r implicated in displacing U1 snRNP from the 5' splice site.

22 reside before its release from the pre-mRNA 5' splice site.

23 philic attack of the branch adenosine at the 5' splice site.

24 iting hnRNP A1 to a sequence upstream of the 5' splice site.

25 x at the branchpoint and the U1 snRNP at the 5' splice site.

26 effect by promoting utilization of the weak 5' splice site.

27 of the spliceosomal U1 snRNP to a suboptimal 5' splice site.

28 due to the SMN2 C>T mutation and suboptimal 5' splice site.

29 We propose a mechanism for selection of the 5' splice site.

30 xogenous guanosine nucleophile to attack the 5'-splice site.

31 rgely on the strength of the flanking 3' and 5' splice sites.

32 nes, but also can promote use of alternative 5' splice sites.

33 rotein particle called U1 snRNP engages with 5' splice sites.

34 by the altered splicing of introns with weak 5' splice sites.

35 exon splicing with RBM20 binding near 3' and 5' splice sites.

36 ion of cassette exons, and usage of upstream 5' splice sites.

37 NPs bound to pre-mRNA with one or two strong 5' splice sites.

38 extreme conservation of GpU observed at most 5'-splice sites.

39 fine-tunes relative affinities of mismatched 5'-splice sites.

40 RBM25 stimulated proapoptotic Bcl-x(S) 5' splice site (5' ss) selection in a dose-dependent man

41 intron compensatory interactions between the 5' splice site (5'ss) and 3' splice site (3'ss) were obs

42 f a complex comprising U1 snRNP bound at the 5' splice site (5'SS) and the Msl5*Mud2 heterodimer enga

43 pre-mRNA splicing is the recognition of the 5' splice site (5'ss) by canonical base-pairing to the 5

44 the major splice donor in the HIV-1 genome, 5' splice site (5'ss) D1, to the first splice acceptor,

45 viral mRNAs, is increased by optimizing the 5' splice site (5'ss) downstream of exon 2 (5'ss D2).

46 an splicing factor, acts at the U1 snRNA and 5' splice site (5'ss) duplex in the pre-mRNA splicing pr

47 ad a major effect on the U1 pattern, whereas 5' splice site (5'ss) mutations had a stronger effect on

48 encer N1 (ISS-N1), located downstream of the 5' splice site (5'ss) of exon 7.

49 The SPO22 intron has a non-consensus 5' splice site (5'SS) that dictates its Nam8/Mer1-depend

50 e splice complex that forms between a pseudo 5' splice site (5'ss) within the negative regulator of s

51 ribonucleoprotein (U1 snRNP) binding to the 5' splice site (5'SS), which is assisted by the Ser/Arg

52 Prp28p has been implicated in unwinding the 5' splice site (5'ss)-U1 small nuclear RNA (snRNA) base-

53 the first catalytic step of splicing at the 5' splice site (5'ss).

54 y of the complex of U1 bound to the pre-mRNA 5' splice site (5'SS).

55 bicistronic pre-mRNA intron 1 contains three 5' splice sites (5' ss) and three 3' splice sites (3' ss

56 model human beta-globin gene with duplicated 5' splice sites (5'ss) and found that PTCs inserted betw

57 GC 5' splice sites (5'ss) are present in approximately 1% o

58 elinase 2 and that ceramides shift the Bcl-x 5'-splice site (5'SS) selection in favor of Bcl-x(S), we

59 tosis, and phenylketonuria, can be caused by 5'-splice-site (5'ss) mutations that are not predicted t

60 ch intron contains three obligate signals: a 5' splice site, a branch site, and a 3' splice site.

61 These include the 5' splice site, a guanosine at position 7, and the 5' sp

62 r data suggest a novel mechanism for exon 16 5' splice site activation in which the binding of RBFOX2

63 -rich proteins facilitate enhancer-dependent 5'-splice site activation.

64 o a consensus sequence close to the Bcl-x(L) 5' splice site, altering the Bcl-x(L)/Bcl-x(S) ratio and

65 rom the self-splicing group I intron binds a 5'-splice site analog (S) and guanosine (G), catalyzing

66 the first step of self-splicing, in which a 5'-splice site analogue (S) and guanosine (G) are conver

67 Comparisons of the affinities of 5'-splice site analogues that form only a subset of base

68 turation of yeast TERs such as an Sm site, a 5' splice site and a branch point, within the newly iden

69 d sequences include a u-G wobble pair at the 5' splice site and a guanosine in the omega position at

70 Interestingly, mutating BDF2 5' splice site and branch point consensus sequences part

71 involves U6atac and U12 interaction with the 5' splice site and branch site regions of a U12-dependen

72 The FRET measurements suggest that the 5' splice site and branch site remain physically separat

73 signals from the tiling array delineated the 5' splice site and branchpoint site, confirming predicte

74 visiae due to the short distance between the 5' splice site and branchpoint; (ii) the S.pombe B block

75 Furthermore, we found that weak 5' splice site and large intron size are the determining

76 ing due to the activation of the overlapping 5' splice site and not to the changes in the integrase p

77 ce site, which greatly increases use of this 5' splice site and promotes gene expression.

78 ed through non-Watson-Crick pairing with the 5' splice site and the branch-point adenosine.

79 ion, the promoter region interacted with the 5' splice site and the terminator with the 3' splice sit

80 P to the 5' splice site no. 4, even when the 5' splice site and the U1 snRNA have been mutated to obt

81 s an intron in the E6 coding region with one 5' splice site and two alternative 3' splice sites, whic

82 we confirmed that U1 directly hybridizes to 5' splice sites and 5' splice site motifs throughout int

83 t U2AF(59)-insensitive introns have stronger 5' splice sites and higher A/U content.

84 nisms underlie G run-mediated recognition of 5' splice sites and that context and position play a cri

85 with increased affinity to HIV-1 downstream 5' splice sites and to sequences within the first tat co

86 olecular ruler whose other end specifies the 5'-splice site and that this molecular ruler is conserve

87 the requirement for DISE to be close to the 5'-splice site and the ability of DISE to promote bindin

88 (SL4) of the U1 snRNA, which recognizes the 5' splice site, and a component of the U2 small nuclear

89 s), contain cytosine-rich sequences near the 5' splice site, and have suboptimal splice sites in the

90 icing, when the branch site (BS) attacks the 5' splice site, and the second step, when the 5' exon at

91 erated via the utilization of an alternative 5' splice site, and this results in an insertion of 31 a

92 exon skipping, intron retention, alternative 5' splice sites, and alternative 3' splice sites.

93 ncers (ESEs) promoted use of intron-proximal 5' splice sites, and tethering of hnRNP A1 and SF2/ASF p

94 ting that non-Watson-Crick base pairs at the 5' splice site are acting in concert with other factors

95 We have investigated the reasons why 5' splice sites are not fixed in complex E, using single

96 liceosomes assembled on reporter genes whose 5' splice sites are predicted to bind poorly at the spli

97 plicated the U1 snRNP and recognition of the 5' splice site as key elements in Mer1p-activated splici

98 ons destabilizing a stem-loop protecting the 5' splice site at the E10/intron 10 junction.

99 g of pre-mRNAs by recognizing and binding to 5' splice sites at exon/intron boundaries.

100 spliced at the E6 and E1/E2 ORFs, where two 5' splice sites at nt 232 and nt 898 and two 3' splice s

101 . crassa TER intron contains a non-canonical 5'-splice site AUAAGU that alone prevents the second ste

102 nteraction of U1 we propose occurs before U1-5' splice site base pairing.

103 In contrast, a non-canonical 5' splice site blocks the second transesterification rea

104 otifs that effectively silenced the proximal 5' splice site both in vitro and in vivo.

105 and A1 induce extended contacts between the 5' splice site-bound U1 snRNA and neighboring exonic seq

106 cific sequence elements, including conserved 5' splice sites, branch points, pyrimidine-rich regions

107 , the U1 snRNP binds properly to the N1 exon 5' splice site but is made inactive by the presence of P

108 inclusion in the context of the native weak 5' splice site, but not the engineered strong 5' splice

109 sed exons in the affected genes contain weak 5' splice sites, but are otherwise indistinguishable fro

110 ntronic U-rich motifs are separated from the 5' splice site by overlapping inhibitory elements.

111 e choice after the initial identification of 5' splice sites by U1 snRNP.

112 Genetic variants within the 5' splice site can cause splicing differences among huma

113 located 100 nucleotides downstream from the 5' splice site can dimerize in vitro through an intermol

114 demonstrated that activation of the Bcl-x(s) 5' splice site can increase the effectiveness of chemoth

115 tein particle (snRNP), bound to a downstream 5' splice site, can positively influence utilization of

116 ing, presumably by interacting with the weak 5' splice site, CCGU.

117 mechanisms for establishing the fidelity of 5' splice site cleavage and exon ligation share a common

118 6 was disabled, these spliceosomes catalyzed 5' splice site cleavage but at a reduced rate.

119 Disrupting stem IIc also exacerbated the 5' splice site cleavage defects of certain substrate mut

120 Prp16 can associate with spliceosomes before 5' splice site cleavage, consistent with a role for Prp1

121 splicing of a genuine substrate until after 5' splice site cleavage, we found that Prp16 can associa

122 istent with a role for Prp16 in proofreading 5' splice site cleavage.

123 in vitro assay sensitive to proofreading of 5' splice site cleavage.

124 xD/H box ATPase that promotes splicing after 5' splice site cleavage.

125 In particular, we detected 5'splice site cleavage by the spliceosome, showing that

126 ing motifs function by altering the U1 snRNP/5' splice site complex in a manner that impairs commitme

127 the presence of U1 snRNPs base-paired to the 5' splice site, components recognizing the 3' splice sit

128 Here, we test the 3' and 5' splice site contributions towards efficient exon reco

129 e of these mutants (NLD2up), the sequence of 5' splice site D2 was changed to a consensus splice dono

130 splice sites that define exon 3, 3'ss A2 and 5' splice site D3.

131 ocking the N1 splice sites, PTB prevents the 5' splice site-dependent assembly of U2AF into the E com

132 The data show that recognition of the DM20 5' splice site depends on G run-mediated recruitment of

133 es where Watson-Crick base pairs form at the 5'splice site do we see degradation of TES products at c

134 ck interactions between the branch helix and 5'-splice site dock the branch adenosine into the active

135 However, it is unknown if the 3' or the 5' splice site dominates the exon recognition process.

136 Five 5' splice sites (donor sites) and six 3' splice sites (a

137 uence of U6 snRNA, which base-pairs with the 5'-splice site during catalytic activation, forms a hair

138 also demonstrated that an upstream competing 5' splice site enhances the rate of proximal splicing.

139 e-binding region of U2 is used in trans as a 5' splice site for both steps of splicing in vivo.

140 tion between the presence of a non-canonical 5' splice site for ESP and the absence of detectable ESP

141 rm owing to competition with a reconstituted 5' splice site formed at the RS-site after the first spl

142 sion) assays show that part of the regulated 5'-splice site forms intramolecular base pairs that are

143 new splicing regulatory element, an upstream 5' splice site functioning as a splicing enhancer.

144 no-specific AGAA-rich ESEs coupled to a weak 5'-splice site functions as a responsive gauge.

145 Exon 16 possesses a weak 5' splice site (GAG/GTTTGT), which when strengthened to

146 ding on cassette exons: binding close to the 5' splice site generally promoted exon inclusion, wherea

147 e S. pombe TER intron contains the canonical 5'-splice site GUAUGU, the N. crassa TER intron contains

148 studied the most common form of alternative 5' splice sites-GYNNGYs (Y = C/T), where both GYs can fu

149 abilize a stem loop structure at the exon 10 5'-splice site; however, the existence of this stem loop

150 e oligonucleotides (ASO) directed toward the 5' splice site I promote the expression of the PKCdeltaV

151 fied a U-rich intronic enhancer flanking the 5' splice site (IE1), and a bipartite exonic enhancer/su

152 with PKCdelta minigene promoted selection of 5' splice site II.

153 ' splice site, but not the engineered strong 5' splice site, implying that RBFOX2 achieves its effect

154 We identify a G-rich enhancer (M2) of DM20 5' splice site in exon 3B and show that individual G tri

155 lement located immediately downstream of the 5' splice site in intron 7.

156 determined that U1 snRNP interacts with the 5' splice site in the downstream exon even in the absenc

157 M20 ratio and, when placed between competing 5' splice sites in an alpha-globin minigene, direct hnRN

158 we determined that two competing alternative 5' splice sites in exon 12 yield Esrp1 isoforms with dif

159 ed by oligodendrocytes (OLs) by selection of 5' splice sites in exon 3.

160 U1 snRNAs associate with 5' splice sites in the form of ribonucleoprotein particl

161 19, and IE17.5 transcripts utilized the same 5'-splice site in exon 3.

162 The P1 extension forms at the 5'-splice site in the first step of self-splicing, and P

163 hnRNPH fusion protein downstream of the DM20 5' splice site increased DM20 splicing, whereas MS2-hnRN

164 r initial recruitment of U1snRNP to the DM20 5' splice site independent of the strength of the splice

165 s rely on hyperstabilization of the U6 snRNA-5' splice site interaction to impede the 2nd step of spl

166 1C and Cbp80p to help stabilize the U1 snRNP-5' splice site interaction.

167 Interestingly, the 5' splice site is required for MBNL1-mediated activation

168 In summary, AS at GYNNGY 5' splice sites is primarily splicing noise, and seconda

169 Alternative splicing of competing 5' splice sites is regulated by enhancers and silencers

170 nstream and potentially competitive stronger 5'-splice site is actively repressed.

171 The 5'-splice site is cleaved but remains close to the catal

172 nces DM20 splicing, whereas close to the PLP 5' splice site, it inhibits PLP splicing.

173 n exons and introns is more complex than for 5' splice sites, largely owing to sequence constraints u

174 s; the third mutation activated a downstream 5' splice site leading to pseudoexon inclusion of a port

175 poly(A) signal or increasing the strength of 5' splice site led to the predominant production of hERG

176 ng from a trans-splicing reaction in which a 5' splice site-like sequence in the universally conserve

177 lements that could silence a proximal strong 5' splice site located downstream of a weakened 5' splic

178 that retained introns tend to have a weaker 5' splice site, more Gs in their poly-pyrimidine tract a

179 1 directly hybridizes to 5' splice sites and 5' splice site motifs throughout introns and found that

180 A rescues splicing of certain branchpoint or 5' splice site mutants in which U snRNA base-pairing has

181 des most unspliced precursors generated by a 5' splice site mutation in RPS10B, and limits RPS29B uns

182 sense mutations, 9 frameshift mutations, and 5 splice-site mutations.

183 ently, certain exons are more susceptible to 5' splice site mutations.

184 tion of a similar transgene with a consensus 5' splice site near the upstream boundary of the inserti

185 U1 snRNP binding to the 5' splice site no. 4 is required for splicing of the rev

186 y for the recruitment of the U1 snRNP to the 5' splice site no. 4, even when the 5' splice site and t

187 nd binding of the U1 snRNP to the downstream 5' splice site no. 4.

188 We demonstrated that neither the 5' splice site nor exon 11 sequences are required for MB

189 binding assays, shows that the selection of 5'-splice site nucleotides by U1 snRNP is achieved predo

190 short RNA duplex is established between the 5' splice site of a pre-mRNA and the 5' end of the U1 sn

191 PKCdeltaVIII via utilization of a downstream 5' splice site of exon 10 on PKCdelta pre-mRNA.

192 tations were identified over time except for 5' splice site of exon 10.

193 onal only in the context of the natural weak 5' splice site of exon 2; they are 1) a uridine-rich enh

194 U-rich intronic sequence (IAS1) adjacent the 5' splice site of exon IIIb.

195 (CBC), preferentially affecting selection of 5' splice site of first introns.

196 of HSF4 showed a homozygous mutation in the 5' splice site of intron 12 (c.1327+4A-->G), which cause

197 a stable stem-loop that includes the normal 5' splice site of intron 5 and is flanked by two alterna

198 tation is due to a G --> A transition at the 5' splice site of intron 6 of SHM1, causing aberrant spl

199 sequence in the U1 snRNA, which binds to the 5' splice site of introns, effectively reversing the rol

200 teractions within U1 snRNP, and show how the 5' splice site of pre-mRNA is recognised by U1 snRNP.

201 ive splicing is a stem-loop structure at the 5' splice site of tau exon 10.

202 reby increasing the access of U1snRNP to the 5' splice site of tau exon 10.

203 r of miRNAs occurs in close proximity to the 5' splice site of the previously identified 7.2-kb stabl

204 When the unregulated 5' splice site of the upstream exon 3 is present, U2AF b

205 We proved that the 5' splice sites of CD46 cassette exons 7 and 8 encoding

206 S1+1G-->A) which is predicted to destroy the 5'-splice site of exon 1 and remove the start methionine

207 utations in tau are silent, are found at the 5'-splice site of exon 10, and lead to increased inclusi

208 TRA/TRA2 could activate the female-specific 5'-splice site of fru.

209 se that the UAYYUU motif hybridizes with the 5' splice site on the SL2 RNA to recruit the SL to the p

210 well as the positive effect of a downstream 5' splice site on trans-splicing in nematode extracts co

211 ns and demonstrated that mutation of the E1B 5' splice site or branchpoint abrogates intrasplicing.

212 site, with only interactions closest to the 5' splice site persisting to the 5' exon intermediate an

213 oned redundantly to protect an exon from its 5' splice site polymorphism.

214 s widespread context-dependent robustness to 5' splice site polymorphisms in human transcriptomes.

215 fied signals that could modify the impact of 5' splice site polymorphisms, most notably a strong 3' s

216 ption-coupled splicing of RNAs in which weak 5' splice sites predominate, enabling diversification of

217 nteractions with the 5' exon adjacent to the 5' splice site, prior to the first step of splicing.

218 at 7 out of 16 base pair combinations at the 5' splice site produce appreciable TES product.

219 e-messenger RNA (pre-mRNA) splicing requires 5' splice site recognition by U1 small nuclear RNA (snRN

220 regulate the PLP/DM20 ratio by reducing DM20 5' splice site recognition by U1snRNP.

221 nd timing of snRNA interactions required for 5' splice site recognition prior to the first step of pr

222 f splicing could reveal important signals of 5' splice site recognition.

223 snRNP 70K protein, a key protein involved in 5' splice site recognition.

224 U1 snRNP plays a critical role in 5'-splice site recognition and is a frequent target of a

225 n, and that this interaction interferes with 5'-splice-site recognition by the U1 snRNP.

226 finally contacts U1-C, which is crucial for 5'-splice-site recognition.

227 ssembly and suggests a possible mechanism of 5'-splice-site recognition.

228 iple steps by re-splicing at ratchet points--5' splice sites recreated after splicing.

229 Thus the GA 5' splice site represents an extension of the adjacent c

230 endonuclease and show that catalysis at the 5' splice site requires the conserved cation-pi sandwich

231 either 3' splice site or near the downstream 5' splice site, respectively.

232 SM induces splicing of STAT1 at a novel 5' splice site, resulting in a STAT1 mRNA incapable of p

233 al U1-snRNP proteins in governing the fungal 5' splice site RNA-RNA interaction compared to the numbe

234 ly, untranslated region activated HPV16 late 5'-splice site SD3632 and resulted in production of HPV1

235 NAs that bypassed the suppressed HPV16 late, 5'-splice site SD3632; produced elevated levels of RNA-b

236 Alternative 5' splice site selection is one of the major pathways re

237 RCE 1, functions to regulate the alternative 5' splice site selection of Bcl-x pre-mRNA, and is requi

238 A cis-elements in regulating the alternative 5' splice site selection of Bcl-x pre-mRNA, we developed

239 nts using pre-mRNAs that undergo alternative 5' splice site selection or alternative exon inclusion.

240 To identify the cis-elements involved in 5' splice site selection we cloned a minigene, which inc

241 totic Bcl-x(L), are produced via alternative 5' splice site selection within exon 2 of Bcl-x pre-mRNA

242 at the level of RNA splicing via alternative 5' splice site selection within exon 2 to produce either

243 ry demonstrated that ceramide regulated this 5' splice site selection, inducing the production of Bcl

244 Because snRNP70 is a key early regulator of 5' splice site selection, our results suggest a model in

245 and antiapoptotic Bcl-x(L), via alternative 5' splice site selection.

246 site with a concomitant increase in Bcl-x(s) 5' splice site selection.

247 lished the ability of ceramide to affect the 5' splice site selection.

248 The lariat is important for the fidelity of 5' splice-site selection and consists of a 2'-5' phospho

249 r, a double-point mutation in the NRS pseudo-5' splice site sequence converted it into a functional 5

250 of human U1C fail to bind RNA containing the 5' splice site sequence, in contrast to what has been re

251 The unique N. crassa TER 5'-splice site sequence is evolutionarily conserved in T

252 urther show that alternative exons with weak 5' splice site sequences specifically show a strong corr

253 ched motif downstream of exons unaffected by 5' splice site SNPs.

254 n of this additional SR protein contacts the 5' splice site specifically in the mature spliceosome.

255 we have previously demonstrated that a weak 5' splice site (ss) serves as the major cause of skippin

256 suboptimal features, which cause the intron 5' splice site (ss) to interact weakly with U1 snRNA and

257 ription/splicing factor, associates with the 5' splice site (SS) within large complexes present in He

258 The NRS acts as a pseudo 5' splice site (ss), and serine-arginine (SR) proteins,

259 A splicing, when the branch site attacks the 5' splice site (SS), and the second step, when the 5' ex

260 spliceosome complexes revealed mechanisms of 5'-splice site (ss) recognition, branching, and intron r

261 alternative exons than either the 3' or the 5' splice site strength alone.

262 ach, we demonstrate that the combined 3' and 5' splice site strengths of internal exons provide a muc

263 hPrp8, did not cross-link to the NRS pseudo-5' splice site, suggesting that the tri-snRNP complex wa

264 es tend to be single stranded, and effective 5' splice sites tend to be double stranded.

265 and U1/U11 snRNPs and functions as a pseudo-5' splice site that interacts with and sequesters 3' spl

266 1 pre-mRNA, directing utilization of a novel 5' splice site that is used only in the presence of SM.

267 on, as well as strong secondary structure at 5' splice sites that correlates with unspliced events.

268 he spliceosome work together to identify the 5 splice site, the 3 splice site, and the branchsite (BS

269 ts an extension of the adjacent conventional 5' splice site, the first natural example of such a comp

270 lose proximity to either the DM20 or the PLP 5' splice site, the ISE recruits U1snRNP to both splice

271 abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third

272 2 increases U1 snRNP recruitment to the weak 5' splice site through direct interaction between its C-

273 ice site, a guanosine at position 7, and the 5' splice site-to-branch point sequence context.

274 stacked or aligned in parallel, bringing the 5'-splice site together with the 3'-splice site and cata

275 II promoter, a neomycin-resistance gene, and 5'-splice site) typically produced mutagenized clones in

276 tating splice site selection than the actual 5' splice site/U1 snRNA base pairing potential.

277 prevents its interaction with the authentic 5' splice site upstream of the NRS.

278 the Ralgps2 mRNA and a shift in alternative 5' splice site usage in the Bptf mRNA.

279 ied out a systematic analysis of alternative 5' splice site usage using in vitro splicing assays.

280 Alternative 5' splice site utilization in the minigene was promoted

281 ownstream of a heterologous exon with a weak 5' splice site was capable of repressing exon inclusion.

282 the patterns of nuclease protection at this 5' splice site, we find that the interaction of U1 is al

283 Weak U1 snRNA interactions with the 5' splice site were significantly rescued by the proximi

284 plex E most transcripts with two alternative 5' splice sites were bound by two U1 snRNPs.

285 , except for those that were adjacent to the 5' splice site where they had the opposite effect.

286 rved sequence element (alpha) located near a 5' splice site, which greatly increases use of this 5' s

287 plicing sequence in intron 2 adjacent to the 5' splice site, which is essential in regulating exon 2

288 n unusual splice-site mutation in the exon 1 5' splice site, which leads to loss of the Kozac sequenc

289 interaction between U6 snRNA and the pseudo 5' splice site, which was shown previously to bind U1 sn

290 omopolymers (polyribonucleotides) and to the 5'-splice site, which overrides the binding of SC35 to t

291 s prevent binding of U1 and U6 snRNPs to the 5' splice site, while TIAR increases binding.

292 by diminishing the selection of the Bcl-x(L) 5' splice site with a concomitant increase in Bcl-x(s) 5

293 docking, and restructures the pairing of the 5' splice site with the U6 snRNA ACAGAGA region.

294 ms, PLP1 and DM20, due to alternative use of 5' splice sites with the same acceptor site in intron 3.

295 of KDR is activated by blocking the upstream 5' splicing site with an antisense morpholino oligomer.

296 rosslinking group is placed further from the 5' splice site, with only interactions closest to the 5'

297 his sequence shifts splicing to a downstream 5' splice site within a heterologous pre-mRNA substrate

298 nuclear RNA and ten proteins, recognize the 5' splice site within precursor messenger RNAs and initi

299 tract sequence motif downstream of a pseudo 5' splice site within the previously characterized intro

300 IE9 utilized a cryptic 5'-splice site within exon 3.