ライフサイエンスコーパス: 5'-exonuclease

1 ins (PCBPs) to protect uncapped PV mRNA from 5' exonuclease.

2 er CPSF-73 functions as an endonuclease or a 5' exonuclease.

3 shift mutations in TREX1, which encodes a 3'-5' exonuclease.

4 element and which blocks the action of a 3'-5' exonuclease.

5 omain did not function independently as a 3'-5' exonuclease.

6 ransferase, and primase, and is also a 3' to 5' exonuclease.

7 le proofreading errors with its intrinsic 3' 5' exonuclease.

8 the parasite extracts functioned as a 3' to 5' exonuclease.

9 d to that predicted for the activity of a 3'-5' exonuclease.

10 n could be subject to proofreading by a 3'-->5' exonuclease.

11 We recently showed that hWRN is also a 3'-->5' exonuclease.

12 this block is specific for a different 3'-to-5' exonuclease.

13 y(U) to protect RNA substrates from the 3'-->5' exonuclease.

14 Q DNA helicase family that contains a 3' --> 5'-exonuclease.

15 olytic processing by a combination of 3' --> 5' exonucleases.

16 sidRNA production depends on distributive 3'-5' exonucleases.

17 TREX1 and TREX2 genes encode mammalian 3'-->5' exonucleases.

18 y factor in their rapid degradation by 3' to 5' exonucleases.

19 created yeast strains lacking specific 3' to 5' exonucleases.

20 t these recombinant proteins are active 3'-->5' exonucleases.

21 regulate histone mRNA metabolism, and the 3'-5' exonuclease 3'hExo trims its 3'-end after processing.

22 ultiple enzymatic activities including 3' to 5' exonuclease, 3' to 5' helicase, and ssDNA annealing.

23 g DNA molecules by the concerted action of a 5' exonuclease, a DNA polymerase and a DNA ligase.

24 Biochemically, the WRN helicase and 3' to 5' exonuclease act simultaneously and cooperate to relea

25 Through this 3'-5' exonuclease action the total 5S RNA of the mutant pos

26 amma) shares conserved DNA polymerase and 3'-5' exonuclease active site motifs with Escherichia coli

27 rimer terminus between the polymerase and 3'-5' exonuclease active sites of the mutant proteins was a

28 To discern how the polymerase and 3' --> 5' exonuclease activities contribute to the high fidelit

29 n of mismatch-specific endonuclease and 3'-->5' exonuclease activities in the reaction mixture.

30 possessed very low DNA polymerase and 3' to 5' exonuclease activities in vitro.

31 e used to test whether 5' -->3' and/or 3' -->5' exonuclease activities mediated degradation.

32 find that both the polymerization and 3' --> 5' exonuclease activities of gp43 are totally inhibited

33 to inhibit both the DNA synthesis and 3' to 5' exonuclease activities of polymerases delta and epsil

34 catalytic centers for both polymerase and 3'-5' exonuclease activities that are separated by about 35

35 NA polymerases with a range of attenuated 3'-5' exonuclease activities was constructed from a chimeri

36 PolD1) possessing both DNA polymerase and 3'-5' exonuclease activities, and a smaller subunit (PolD2)

37 otein contains 3'-phosphodiesterase and 3'-->5' exonuclease activities, and mutation of the active-si

38 none of these processing steps involves 3'-->5' exonuclease activities, the requirement for the exoso

39 activities as well as deadenylating and 3'-->5' exonuclease activities.

40 amily and contains 3'-->5' helicase and 3'-->5' exonuclease activities.

41 contains both RNA and single-stranded DNA 3'-5' exonuclease activities.

42 possesses both 3' --> 5' helicase and 3' --> 5' exonuclease activities.

43 ein (WRN) that has ATPase, helicase and 3'-->5' exonuclease activities.

44 lypeptide has both 3'-->5' helicase and 3'-->5' exonuclease activities.

45 amily, PolB possesses both polymerase and 3'-5' exonuclease activities.

46 ual contributions of its polymerase and 3'-->5' exonuclease activities.

47 context of a single-nucleotide gap), 3' --> 5' exonuclease activity (in the context of a nick), and

48 protein also possesses metal-dependent 3'-->5' exonuclease activity against single-stranded DNAs and

49 tivity, since reverse transcriptases lack 3'-5' exonuclease activity and generally have low fidelity.

50 AP endonuclease 1, which possesses 3'-->5' exonuclease activity and potentially serves as a proo

51 1 (APE1) prevented TNR expansions via its 3'-5' exonuclease activity and stimulatory effect on DNA li

52 RN-N(70-240)), exhibits the same level of 3'-5' exonuclease activity as the previously described exon

53 nating with flap endonuclease 1, the APE1 3'-5' exonuclease activity cleaves the annealed upstream 3'

54 t the smaller human protein exhibits a 3' to 5' exonuclease activity for small (primarily </=5 nucleo

55 st mismatch excision catalyzed by the 3' --> 5' exonuclease activity further lowers the error frequen

56 11--but a specific function for the Mre11 3'-5' exonuclease activity has remained elusive.

57 e TREX enzymes process DNA as the major 3'-->5' exonuclease activity in mammalian cells.

58 s that POLG1 requires both polymerase and 3'-5' exonuclease activity in the same molecule.

59 e domain of OsCCR4a and OsCCR4b exhibited 3'-5' exonuclease activity in vitro, and point mutation of

60 1B, rOsCAF1G, and rOsCAF1H, all exhibited 3'-5' exonuclease activity in vitro.

61 pletely stalled by the lesion, as its 3' --> 5' exonuclease activity increased significantly and outc

62 hird of the wild-type enzyme and has a 3'-to-5' exonuclease activity indistinguishable from that of w

63 These results demonstrate that 3'-->5' exonuclease activity is an important prerequisite for

64 APE2 3'-phosphodiesterase and 3'-5' exonuclease activity is essential for single-stranded

65 Pol epsilon lacking 3' --> 5' exonuclease activity is less accurate to a degree sug

66 The U7-dependent 5' exonuclease activity is processive and continues degr

67 strand displacement synthesis unless its 3'-5' exonuclease activity is removed.

68 The 3' --> 5' exonuclease activity is shown to be highly dependent

69 However, inactivation of the 3'-5' exonuclease activity is sufficient to allow the polym

70 Recently, the comparatively "minor" 3' to 5' exonuclease activity of Ape1 was found to contribute

71 Furthermore, the 3' --> 5' exonuclease activity of DNA polymerase epsilon or wil

72 y biological function attributed to the 3'-->5' exonuclease activity of DNA polymerases was proofread

73 The 3'-->5' exonuclease activity of hPol further enhances polymer

74 es confirmed that the DNA-binding and 3' --> 5' exonuclease activity of human NDK1 is an intrinsic ac

75 HmtSSB enhances intrinsic 3'-5' exonuclease activity of p53, particularly in hydrolys

76 Finally, the 3' --> 5' exonuclease activity of PolB1 was the highest when 8-

77 ing terminal nucleotide by way of the 3' --> 5' exonuclease activity of polymerase I.

78 is in yeast strains with defects in the 3'-->5' exonuclease activity of replicative DNA polymerases d

79 he involvement of the recently characterized 5' exonuclease activity of RNase J1 and endonuclease act

80 the downstream products were degraded by the 5' exonuclease activity of RNase J1.

81 oordinately to protect the viral genome from 5' exonuclease activity of the host mRNA decay machinery

82 The mechanism of the 3'-5' exonuclease activity of the Klenow fragment of DNA po

83 Abolishment of the 3'-5' exonuclease activity of wild-type pol I increased mut

84 DSBs stimulate the phosphorylation and 3'-5' exonuclease activity of X-Mre11 complex.

85 However, the 3'-5' exonuclease activity on polynucleotide substrates is

86 within single-stranded DNA, as well as a 3'-5' exonuclease activity on single-stranded DNA.

87 evidenced by the significant reduction in 3'-5' exonuclease activity resulting from a Lys(12) to glut

88 plicative polymerases have an efficient 3'-->5' exonuclease activity that excises misincorporated nuc

89 y DNA polymerases also possess a separate 3'-5' exonuclease activity that is used to remove misincorp

90 Mre11 has a 3' to 5' exonuclease activity that results in the release of m

91 cted synthesis of DNA and uses a separate 3'-5' exonuclease activity to edit misincorporated bases.

92 Replicative DNA polymerases possess 3' --> 5' exonuclease activity to reduce misincorporation of in

93 The 3'-5' exonuclease activity was assigned to NM23-H1 by virtu

94 -induced (254 nm) DNA damage, whereas its 3'-5' exonuclease activity was dominant in the suppression

95 When T4 DNA polymerase deficient in 3' to 5' exonuclease activity was employed, electron microscop

96 nge the polymerase activity; however, the 3'-5' exonuclease activity was reduced 2-29-fold, depending

97 We now show that inhibition of MRE11 3'-5' exonuclease activity with Mirin reduces the frequency

98 DNA replication, mediated by an intrinsic 3'-5' exonuclease activity within its PHP domain.

99 with efficient reverse transcriptase and 3'-5' exonuclease activity, a family of mutant DNA polymera

100 ect otherwise unstable transcripts from 3'-->5' exonuclease activity, a phenomenon that may occur nat

101 Initial characterization revealed a 3' to 5' exonuclease activity, and showed additional functiona

102 ndonuclease activity toward 5' flaps and has 5' exonuclease activity, and these activities are mediat

103 present in the protein complex exhibited 3'-5' exonuclease activity, it was incapable of excising th

104 The same enzyme, but lacking 3' to 5' exonuclease activity, permits synthesis to proceed by

105 ngle-strand DNA-binding and processive 3'-to-5' exonuclease activity, respectively.

106 t of an Archaea DNA polymerase possessing 3'-5' exonuclease activity, since reverse transcriptases la

107 both a 3'-->5' helicase activity and a 3'-->5' exonuclease activity, the stimulating activity was fo

108 icative DNA polymerase with an associated 3'-5' exonuclease activity.

109 sence of which severely diminishes its 3' to 5' exonuclease activity.

110 at human NDK5, NDK7, and NDK8 contain 3' --> 5' exonuclease activity.

111 1 (NM23-H1) has been reported to have 3' --> 5' exonuclease activity.

112 on Klenow fragments with and without 3' --> 5' exonuclease activity.

113 Yet the mutants retain robust 3'-->5' exonuclease activity.

114 netics, strand displacement synthesis and 3'-5' exonuclease activity.

115 , both long isoforms were shown to have 3'-->5' exonuclease activity.

116 AN34 also displays enhanced 3'-5' exonuclease activity.

117 lls treated with STI-571 show increased 3'-->5' exonuclease activity.

118 The protein possesses Mg2+-dependent 3' to 5' exonuclease activity.

119 leading to their eventual removal by the 3'-5' exonuclease activity.

120 f the recQ family, it contains a unique 3'-->5' exonuclease activity.

121 possesses single-stranded DNA and/or RNA 3'-5' exonuclease activity.

122 ermediate product is then trimmed by a 3' -->5' exonuclease activity.

123 th 3'-->5' polarity, and also possesses 3'-->5' exonuclease activity.

124 binds to single-stranded DNA and exhibits 3'-5' exonuclease activity.

125 idelity by modulating the proofreading 3' to 5' exonuclease activity.

126 Fourth, both the endonuclease activity and 5'-exonuclease activity of ARTEMIS can be blocked in par

127 recombinant protein we confirmed that the 3'-5'-exonuclease activity of Pol delta can efficiently rem

128 erminated 3'-end of the DNA primer by the 3'-5'-exonuclease activity of Pol gamma were similar (0.01

129 n uncertainty about whether ARTEMIS also has 5'-exonuclease activity on single-stranded DNA and 5'-ov

130 cleavage activity was not required for Exo1 5'-exonuclease activity on the lagging strand daughter D

131 e ability of Pol delta to back up via its 3'-5'-exonuclease activity, a process called idling, mainta

132 high fidelity owing to its intrinsic 3'- to 5'-exonuclease activity, which confers proofreading abil

133 NA editing exonuclease 1 (REX1), exhibits 3'-5'-exonuclease activity.

134 duces both the endonuclease activity and the 5'-exonuclease activity.

135 rase activity but only mildly impairs 3'- to 5'-exonuclease activity.

136 a endonuclease V also possesses non-specific 5'-exonuclease activity.

137 t the three biological functions of the 3'-->5' exonuclease addressed in this study are performed int

138 atalyzes three DNA-dependent reactions: a 3'-5'-exonuclease, an ATPase, and a 3'-5'-helicase.

139 a multifunctional nuclear protein with 3'-->5' exonuclease and 3'-->5' helicase activities.

140 Importantly, PCNA stimulates the 3'-->5' exonuclease and 3'-phosphodiesterase activities of Ap

141 in of Saccharomyces cerevisiae contains 3'-->5' exonuclease and 3'-phosphodiesterase activities, and

142 s a nuclear protein (WRN) which possesses 3'-5' exonuclease and ATPase-dependent 3'-5' helicase activ

143 sequential detection of the activities of 3'-5' exonuclease and DNase I in cell lysates.

144 Although the purified protein exhibits 3' to 5' exonuclease and endonuclease activities in vitro, Mre

145 We therefore examined WRN, a 3'-->5' exonuclease and helicase mutated in Werner syndrome,

146 We found that hRAD9 is a 3' to 5' exonuclease and located the nuclease active site to t

147 f its attendant enzymatic activities, the 3'-5' exonuclease and nucleoside diphosphate kinase, are no

148 ication by ISG20 and PKR depends on their 3'-5' exonuclease and protein kinase activities, respective

149 ciency of the WRN protein that harbors 3' -->5' exonuclease and RecQ-type 3' --> 5' helicase activiti

150 the Escherichia coli Klenow fragment (KF) 3'-5' exonuclease and snake venom phosphodiesterase.

151 Here, we show that the 5'-exonuclease and the endonuclease activities co-purify

152 Third, divalent cation effects on the 5'-exonuclease and the endonuclease parallel one another

153 Human exonuclease 1 (hExo1) possesses both 5'exonuclease and flap endonuclease activities and plays

154 ) and recJ (5'-->3' exonuclease), xonA (3'-->5' exonuclease) and partially dependent on recQ (helicas

155 unit (polymerase), the epsilon subunit (3'-->5' exonuclease), and the theta subunit, in the linear or

156 e is folded into NH(2)- terminal, editing 3'-5' exonuclease, and polymerase domains that are topologi

157 ein complex composed of RET1 TUTase, DSS1 3'-5' exonuclease, and three additional subunits.

158 rmamido-pyrimidine-DNA glycosylase (FPG), 3'-5' exonucleases, and enzymes with template-independent t

159 3'-5' Exonucleases are believed to play an important role i

160 Related exosome complexes of 3'-->5' exonucleases are present in the nucleus and the cytop

161 we identified PNLDC1, an uncharacterized 3'-5' exonuclease, as Trimmer in silkworms.

162 Here, we describe the development of a 5' exonuclease assay for the detection of serogroup Y Ne

163 ng, direct sequencing, fluorescence-detected 5'-exonuclease assays, and hybridization with PNA probes

164 enzyme was originally identified as a 3' --> 5' exonuclease, but we show here that NrnA is bidirectio

165 e have defined NM23-H1 biochemically as a 3'-5' exonuclease by virtue of its ability in stoichiometri

166 ta long noncoding RNAs are protected from 3'-5' exonucleases by highly conserved triple helical struc

167 quired before RNase P and the various 3' --> 5' exonucleases can complete tRNA maturation.

168 TREX1 and TREX2 proteins contain potent 3'-->5' exonucleases capable of functioning in this capacity.

169 The 3'-->5' exonucleases catalyze the excision of nucleoside mono

170 Kinetic parameters for 3'-5' exonuclease cleavage of single- and double-stranded D

171 ggest the existence of a highly conserved 3'-5' exonuclease core domain within Rrp6p.

172 exonuclease III and T4 DNA polymerase's 3'-->5' exonuclease, covers approximately 35 base pairs and i

173 nase associates with exonuclease 1 (Exo1), a 5'-exonuclease crucial for 5'-end resection to mediate D

174 mer extension studies, catalyzed by the 3'-->5' exonuclease-deficient Klenow fragment of Escherichia

175 plate-directed nucleotide addition by the 3'-5' exonuclease-deficient large fragment of Escherichia c

176 bstitutions in the polymerase domain of a 3'-5'-exonuclease-deficient Klenow fragment.

177 ranscript acts by activating exosomal 3' --> 5'-exonuclease degradation activity.

178 reatly elevated in phage deficient in the 3'-5' exonuclease, DexA, suggesting that the length of 3' t

179 nced, and found to be closely related to the 5' exonuclease domain of bacterial DNA polymerase I prot

180 a heterozygous pol3-01 mutation in the 3'-->5' exonuclease domain of DNA polymerase delta.

181 tioning of DNA to the spatially separated 3'-5' exonuclease domain, providing an additional mechanism

182 of the WS protein containing a potential 3'-5' exonuclease domain.

183 The D368A mutation within the 3'-5'-exonuclease domain of the herpes simplex type 1 DNA p

184 , owing to their lack of a proofreading (3'- 5' exonuclease) domain.

185 The 5'-exonuclease domains of the DNA polymerase I proteins

186 al degradosome, which also includes a 3' --> 5' exonuclease, Dss1p.

187 ed an about tenfold attenuation on the 3' to 5' exonuclease efficiency of Ape1.

188 we define the substrate requirements for the 5'-exonuclease enzymes from Thermus aquaticus, Thermus t

189 merase subunit, alpha, the proofreading 3'-->5' exonuclease, epsilon, and a subunit of unknown functi

190 a replicase generally have a more potent 3'-5' exonuclease (exo) activity than A family DNA polymera

191 DNA polymerases (Pol) have an intrinsic 3'-->5' exonuclease (Exo) activity which corrects polymerase

192 failures, result in substitutions in the 3'-5' exonuclease (Exo) domain of the catalytic subunit of

193 site, DNA polymerases have a separate 3' --> 5' exonuclease (exo) editing activity that is involved i

194 despite the presence of an associated 3'- to 5'-exonuclease (exo) activity.

195 y both the 5'-3' exonuclease RecJ and the 3'-5' exonuclease ExoI, observations that suggest the impor

196 other RNA viruses and is mediated by a 3'-->5' exonuclease (ExoN) activity that probably functions i

197 The association of DcpS with 3' to 5' exonuclease exosome components suggests that these tw

198 , and its intriguing regulation of the 3'-to-5' exonuclease exosome subunit suggests a potential inte

199 Genotyping for 10 SNPs by 5'-exonuclease fluorescence assays.

200 extension reactions catalyzed by the 3' --> 5' exonuclease-free (exo(-)) Klenow fragment of Escheric

201 er extension reactions catalyzed by the 3'-->5' exonuclease-free Klenow fragment of Escherichia coli

202 The cytosolic 3'-to-5' exonuclease from chronic lymphocytic leukemia cells w

203 ht into the mechanism for Nfo-catalyzed 3'-->5' exonuclease function and its inhibition by 3'-termina

204 op structure is readily attacked by the 3'-->5' exonuclease function of WRN.

205 -phosphodiesterase, 3'-phosphatase, and 3'-->5'-exonuclease functions specific for the 3' termini of

206 e we identify oligoribonuclease (Orn)-a 3'-->5' exonuclease highly conserved among Actinobacteria, Be

207 ype 9 degrees N polD were used to examine 3'-5' exonuclease hydrolysis activity in the presence of Mg

208 ternucleotide linkages are resistant to 3'-->5' exonuclease hydrolysis, rendering the target DNA resi

209 action was necessary to protect PV mRNA from 5' exonuclease immediately as ribosomes initially traver

210 racts protected RNA substrates from the 3'-->5' exonuclease in a protein-dependent fashion.

211 death process implicating this major 3' --> 5' exonuclease in genomic DNA degradation to minimize po

212 ocks the activity of a highly efficient 3'-->5' exonuclease in HeLa extracts.

213 Both the 5' --> 3' DNA polymerase and 3' --> 5' exonuclease in pol gamma are stimulated 15-20-fold on

214 chemical evidence that Isg20 acts as a 3' to 5' exonuclease in vitro.

215 o dnaQ, encoding the epsilon proofreading 3'-5'-exonuclease, interacts with alpha but does not form a

216 The yeast exosome is a complex of 3'-->5' exonucleases involved in RNA processing and degradati

217 RNase T and RNase PH, the two primary 3' --> 5' exonucleases involved in the final step of 3'-end mat

218 Nase T and/or RNase PH, the two major 3' --> 5' exonucleases involved in the final step of tRNA 3'-en

219 We conclude that the 5'-exonuclease is intrinsic to ARTEMIS, making it releva

220 -stranded DNA and 5'-overhangs, because this 5'-exonuclease is not dependent upon DNA-PKcs.

221 s in different biological matrices show that 5'-exonuclease is the most prevalent nuclease activity i

222 The protein is a single-strand 5'-exonuclease, like its yeast homolog.

223 tural substrates, indicating that the 3' --> 5' exonuclease may contribute to DNA synthesis inhibitio

224 ore than 100 bases per second and has a 3'-->5' exonuclease (nucleotide removing) activity at a separ

225 unstacking, and base excision by the 3' --> 5' exonuclease of bacteriophage T4 (T4 pol) was examined

226 ow that this mutation inactivates the 3' --> 5' exonuclease of poldelta and causes a mutator and canc

227 ea (MNU)-treated DNA templates by the 3' --> 5' exonuclease of T4 DNA polymerase.

228 genetic and biochemical analysis of the 3'-->5' exonuclease of yeast DNA polymerase delta (Pol delta)

229 the XPF-ERCC1 heterodimer, acted as a 3'-to-5' exonuclease on cross-linked DNA in the presence of RP

230 Escherichia coli, the effects of several 3'-5' exonucleases on RecA loading were studied by assaying

231 ally contained a DNA polymerase devoid of 3'-5' exonuclease, or "proofreading", activity blended with

232 The exosome complex of 3'-5' exonucleases participates in RNA maturation and quali

233 everse transcription-PCR and by an automated 5' exonuclease PCR.

234 se activity of T4 DNA polymerase (43Exo), 3'-5' exonucleases, play a role in intron homing.

235 Furthermore, ExoI, ExoIII and the other 3'-5' exonucleases process these DSBs, antagonizing the Rec

236 The 3' --> 5'-exonucleases process DNA ends in many DNA repair path

237 s from these mutants suggested limited 3'-to-5' exonuclease processing from the native 3' end.

238 GRF mutations impact APE2 DNA binding and 3'-5' exonuclease processing, and also prevent efficient AP

239 ibonucleotides escape proofreading by 3' --> 5' exonuclease-proficient Pol epsilon, indicating that r

240 sms (SNPs) that takes advantage of the 3'-to-5' exonuclease proofreading activity of many DNA polymer

241 -deficient (DeltaumuDC) strains if the 3' to 5' exonuclease proofreading activity of the Pol III epsi

242 Saccharomyces cerevisiae that possess 3' --> 5' exonuclease proofreading activity.

243 ily of proteins that have homology to the 3'-5' exonuclease proofreading subunit (DnaQ) of E. coli DN

244 The 5'-exonuclease Rat1 degrades pre-rRNA spacer fragments a

245 on of the ARE or inactivation of the nuclear 5'-exonuclease Rat1.

246 that the N420 side chain facilitates the 3'-5' exonuclease reaction by introducing strain into the b

247 polymerases based on the analysis of its 3'-5' exonuclease reaction in the context of crystal struct

248 ree-prime repair exonuclease-1, a host 3' to 5' exonuclease, reduced IFN-beta expression significantl

249 and characterization of a nonexosomal, 3'-->5' exonuclease required for SL RNA 3'-end formation in T

250 Here we identify Dis3l2 as the 3'-5' exonuclease responsible for the decay of uridylated p

251 Here, we show that the 3'-->5' exonuclease RNase II plays an important role in RelE-

252 ecificity endoribonuclease RNase III and the 5' exonuclease RNase J1 are not essential in the Gram-po

253 that does not involve any of the six 3' --> 5' exonucleases (RNase T, RNase PH, RNase D, RNase BN, R

254 ure revealed only one protein, another 3'-to-5' exonuclease, RNase II.

255 rtitioning of the DNA substrates into the 3'-5' exonuclease site by 3-7-fold, relative to the perfect

256 the level of partitioning of DNA into the 3'-5' exonuclease site by 8- and 18-fold, respectively, rel

257 s to promote partitioning of DNA into the 3'-5' exonuclease site decreased in the following order: G

258 titioning of bulged DNA substrates to the 3'-5' exonuclease site relative to that of properly base-pa

259 omotes transfer of a DNA substrate to the 3'-5' exonuclease site, suggesting that the premutational i

260 ielding a polymerizing complex, or to the 3'-5' exonuclease site, yielding an editing complex.

261 cally defined DNA-protein contacts at the 3'-5' exonuclease site.

262 oximity to a DNA 3' terminus bound at the 3'-5' exonuclease site.

263 imer/template to the 5'-3' polymerase and 3'-5' exonuclease sites of KF.

264 ctional occupancies of the polymerase and 3'-5' exonuclease sites of the enzyme for DNA substrates wi

265 revealed its association with Rrp6p, a 3'-->5' exonuclease specific to the nuclear exosome fraction.

266 ation of the DCP does not depend on the Xrn2 5' exonuclease, suggesting that CPSF-73 degrades the DCP

267 TREX2 is an autonomous nonprocessive 3' --> 5' exonuclease, suggesting that it maintains genome inte

268 tein is homologous with members of the 3' to 5' exonuclease superfamily that includes RNases T and D,

269 Furthermore, TREX2 (a 3'-->5' exonuclease) suppressed identical repeat fusion but e

270 B in tamarins was followed using a real-time 5' exonuclease (TaqMan) reverse transcription-PCR assay

271 approximately threefold and was the only 3'-5' exonuclease tested that did not deleteriously affect

272 nt holoenzyme contains DNA polymerase and 3'-5' exonuclease that are stimulated substantially by both

273 e, in vitro and in vivo, that TdTL is a 3'-->5' exonuclease that catalyzes the deletion of nucleotide

274 TREX1 is a potent 3' --> 5' exonuclease that degrades single- and double-stranded

275 DNA replication depends on a proofreading 3'-5' exonuclease that is associated with the replicative D

276 solated as a major nuclear DNA-specific 3'-->5' exonuclease that is widely distributed in both prolif

277 Exo5 is a monomeric 5' exonuclease that releases dinucleotides as products.

278 re with replication, whereas TREX2 is a 3'-->5' exonuclease that removes 3' mismatched nucleotides an

279 Trex2 is a 3' --> 5' exonuclease that removes 3'-mismatched sequences in a

280 aliana AtRrp4p is shown to be an active 3'-->5' exonuclease that requires a free 3'-hydroxyl and degr

281 ein complex consisting of a variety of 3'-to-5' exonucleases that functions both in 3'-to-5' trimming

282 Human EXOG (hEXOG) is a 5'-exonuclease that is crucial for mitochondrial DNA rep

283 We showed that these are U-specific 3'-5'-exonucleases that are inhibited by base pairing of 3'

284 stinct forms of a complex containing 10 3'-->5' exonucleases, the exosome, are found in yeast nucleus

285 y increased the ability of the enzyme's 3'-->5' exonuclease to remove mispaired 3' bases in a primer

286 ations identified in genes encoding the 3'-->5' exonuclease TREX1 and the three subunits of the RNASE

287 se DNA fragments and that the cytoplasmic 3'-5' exonuclease Trex1 is required for their degradation.

288 We now find the 3'-to-5' exonuclease TREX1, but not its close homolog TREX2, i

289 ntified the cDNA sequences encoding two 3'-->5' exonucleases (TREX1 and TREX2) from mammalian cells.

290 autonomous and DNA polymerase-associated 3'-5' exonucleases using a lacZ forward mutation assay.

291 A number of 3'-5' exonucleases were found to lower the error rate of MM

292 component of the exosome, a complex of 3'-to-5' exonucleases whose subunits have been implicated in 5

293 re members of a family of structure-specific 5'-exonucleases with similar function but limited sequen

294 how that the mouse WRN (mWRN) is also a 3'-->5' exonuclease, with substrate specificity similar to th

295 Transfected HCV RNA is degraded by both the 5' exonuclease Xrn1 and 3' exonuclease exosome complex,

296 dsRNA effector pathways and/or the cellular 5' exonuclease Xrn1 were prepared by CRISPR-Cas9 gene ed

297 Uncapped mRNAs are typically degraded by the 5' exonuclease XRN1.

298 ynthetic-lethal with loss of the cytoplasmic 5'-exonuclease Xrn1, indicating block of mRNA turnover,

299 We find that in mammalian cells, the 5' exonuclease Xrn2 plays a major role in both maturatio

300 the combination of I709F and lack of the 3'-5' exonuclease yielded a 400-fold increase.