ライフサイエンスコーパス: A. vinelandii

1 A. vinelandii also encodes a protein designated NfuA, wh

2 A. vinelandii dsbA mutant strains were impossible to cha

3 NifX purified from an A. vinelandii nifB strain showed a different electrophor

4 Here, we report a systematic analysis of an A. vinelandii UW97 mutant and show that, unlike A. vinel

5 Here we show that an A. vinelandii strain expressing the V-nitrogenase is cap

6 This contrasts with an A. vinelandii strain described previously which is unabl

7 rity of the vnfEN genes of A. variabilis and A. vinelandii was not strong.

8 ype Fe protein to the crude extracts made by A. vinelandii UW97.

9 olic regulon, was replaced by the contiguous A. vinelandii iscS, iscU, iscA, hscB, hscA, fdx, and isc

10 on required crude extract of the DeltanifHDK A. vinelandii strain CA12, Fe protein and MgATP.

11 We also find that the newly discovered A. vinelandii siderophore vibrioferrin is almost complet

12 non-polar insertion mutations within either A. vinelandii iscS or hscA revealed that such mutations

13 In addition to Fe, A. vinelandii siderophores are used for the acquisition

14 tudy of siderophore production in N2 -fixing A. vinelandii under a variety of trace metal conditions.

15 e its electron-flux inhibition with all four A. vinelandii nitrogenases, supporting the suggestion th

16 n transfer, to a similar extent for all four A. vinelandii nitrogenases.

17 alysis of the purified apodinitrogenase from A. vinelandii DJ54.

18 tion of a fully active species of FeVco from A. vinelandii.

19 cy results from reduction of Fe protein from A. vinelandii (Av2) to the all-ferrous oxidation state (

20 geneous, His-tagged form of VFe protein from A. vinelandii.

21 on of His-tagged MoFe proteins purified from A. vinelandii nifZ and nifZ/nifB deletion strains DJ1182

22 mutant forms of dinitrogenase reductase from A. vinelandii that are affected in various protein activ

23 ature of the iscSUA gene cluster region from A. vinelandii is that E. coli genes previously designate

24 Molybdenum biochemistry in A. vinelandii reveals unexpected mechanisms and a new ro

25 IscS, IscU, HscBA, and Fdx are essential in A. vinelandii and that their depletion results in a defi

26 Here, BchL was expressed in A. vinelandii and purified to homogeneity using an engin

27 sulfur for iron-sulfur cluster formation in A. vinelandii is supported by the presence of a cysE-lik

28 in encoded by nifD-K translational fusion in A. vinelandii is a large protein (as determined by Weste

29 hat glnK, like glnA, is an essential gene in A. vinelandii.

30 a general purpose monothiol glutaredoxin in A. vinelandii, was monitored by circular dichroism spect

31 pparent co-expression of iscSUA and hscBA in A. vinelandii indicate that the proposed chaperone funct

32 existence of a redox regulatory mechanism in A. vinelandii that controls the rate of expression and m

33 e stress or modification of Fe metabolism in A. vinelandii.

34 ployed to confirm that the Nif- phenotype in A. vinelandii UW97 is exclusively due to the substitutio

35 at there is a putative regulatory protein in A. vinelandii that binds specifically upstream of the -3

36 The presence of a V storage protein in A. vinelandii was also investigated.

37 -dependent regulation and gene redundancy in A. vinelandii, it has been difficult to determine the pr

38 Here, the roles of GlnK and GlnK-UMP in A. vinelandii were studied to determine whether the Nif

39 of nitrogenase genes (nif, anf, and vnf) in A. vinelandii, and one ammonia monooxygenase gene (amoA)

40 r new prospects for the wider application of A. vinelandii as a host for the production and character

41 ne contained within the major nif cluster of A. vinelandii previously designated orf6.

42 also located within the nif gene cluster of A. vinelandii.

43 These results might suggest that extracts of A. vinelandii contain a physiological source of reductan

44 lementation of these assays with extracts of A. vinelandii DJ42.48 (DeltanifENX DeltavnfE) results in

45 species of VNFG are observed in extracts of A. vinelandii strains that accumulate dinitrogenase 2, w

46 or intact cluster transfer to an apo form of A. vinelandii aconitase A, a member of the monomeric deh

47 own that the [4Fe-4S] cluster-loaded form of A. vinelandii IscU, but not the [2Fe-2S] cluster-loaded

48 The redox and nitrogen-sensing functions of A. vinelandii NIFL are therefore separable and are discr

49 unlike A. vinelandii DJ54, the nifH gene of A. vinelandii UW97 has no deletion in either coding sequ

50 Purified GroEL of A. vinelandii alone or in combination with MgATP and Fe

51 ave no effects on the diazotrophic growth of A. vinelandii while WO(3) NPs are highly detrimental to

52 L clusters to the biosynthetic machinery of A. vinelandii for further maturation into the M clusters

53 elective pressure against the maintenance of A. vinelandii iscS or hscA knock-out mutations and sugge

54 erefore, it seems that the Nif- phenotype of A. vinelandii UW97 is caused by a general structural dis

55 tation responsible for the Nif- phenotype of A. vinelandii UW97 is the substitution of a non-conserve

56 alanine did not affect the Nif phenotype of A. vinelandii.

57 Genome-wide transcription profiling of A. vinelandii cultured under nitrogen-fixing conditions

58 Therefore, the in vivo role of A. vinelandii DsbA is unknown, but it may function to fo

59 re we report the complete genome sequence of A. vinelandii DJ, which has a single circular genome of

60 aining extracts from a DeltanifHDK strain of A. vinelandii CA12 along with FeMoco, Fe protein, and Mg

61 ntroduced into wild type and FdI- strains of A. vinelandii.

62 in both wild-type and delta nifW strains of A. vinelandii.

63 d DNA sequence analysis of a 7,886-base pair A. vinelandii genomic segment that includes the iscS gen

64 with exquisitely oxygen-sensitive processes, A. vinelandii is specialized in terms of its complement

65 rologous Clostridium pasteurianum Fe protein-A. vinelandii MoFe protein complex.

66 Since A. vinelandii DJ54 is a delta nifH strain, this strain i

67 E2oCD 24mer in comparison to the less stable A. vinelandii E2pCD 24mer.

68 We show that A. vinelandii has two clusters of rnf-like genes: rnf1,

69 eam of Escherichia coli fpr, suggesting that A. vinelandii may have a SoxS-like regulatory system and

70 The A. vinelandii dsbA gene was expressed in Escherichia col

71 The A. vinelandii DsbA protein contains the well-conserved m

72 As described initially for the A. vinelandii Fe-protein in a different crystal form at

73 on of reductant is required, however, if the A. vinelandii cell-free extract is chemically oxidized p

74 gene, herein designated fdxD, that is in the A. vinelandii iscSUA operon that encodes proteins involv

75 rrangement observed in the structures of the A. vinelandii and C. pasteurianum Fe-proteins indicate t

76 eporter gene was placed under control of the A. vinelandii fpr promoter and introduced into wild type

77 Inspection of the A. vinelandii genome indicates that such recruitment of

78 was used for the functional analysis of the A. vinelandii isc genes, whose products are involved in

79 Here, we report the identification of the A. vinelandii protein that binds specifically to the par

80 dients during turnover demonstrates that the A. vinelandii cytochrome bd oxidase is coupled to energy

81 to the vnfDK genes just upstream than to the A. vinelandii vnfEN genes.

82 sults show that the toxicity of WO(3) NPs to A. vinelandii is due to an interference with the catecho

83 350 times tighter than that of the wild-type A. vinelandii nitrogenase complex and at least 20 times

84 ructure of the complex between the wild-type A. vinelandii nitrogenase proteins stabilized with ADP.A

85 vinelandii UW97 mutant and show that, unlike A. vinelandii DJ54, the nifH gene of A. vinelandii UW97

86 e relative levels of FdIV and FdI in various A. vinelandii strains leading to the conclusion that FdI