ライフサイエンスコーパス: ACBP

1 ACBP binds very-long-chain acyl-CoA esters, which is req

2 ACBP displays an atypical compliance along two nearly or

3 ACBP does not cross the blood-brain barrier.

4 ACBP increases the activity of ceramide synthase 2 (CerS

5 ACBP interacts with the alpha1-subunit of the GABA(A) re

6 ACBP lacks a signal sequence for secretion through the e

7 ACBP overexpression in COS-7 or rat hepatoma cells enhan

8 ACBP was localized to Muller glial cells by hybridizatio

9 ACBP/DBI has been identified as the endogenous PBR ligan

10 ual triple fluorescent-labeled (HNF-4 alpha, ACBP, and luciferase) rat hepatoma cells showed a high c

11 alpha in vitro and in intact cells, although ACBP expression level directly correlated with HNF-4 alp

12 denaturant concentrations, unfolded CI2 and ACBP are more compact and display larger fluctuations th

13 ection increased ACBP mRNA transcription and ACBP protein expression.

14 In contrast, cotransfection with antisense ACBP expression vector inhibited transactivation.

15 fluorescence dynamics demonstrated that apo-ACBP was an ellipsoidal protein (axes of 15 and 9 A) who

16 d an intermolecular distance of 53 A between ACBP and HNF-4 alpha in rat hepatoma cell nuclei.

17 lciparum ACBP-acyl-CoA complex and of bovine ACBP in two crystal forms.

18 ng specificity different from that of bovine ACBP.

19 Overall, the bovine ACBP crystal structures are similar to the NMR structure

20 f very-long acyl chain ceramide synthesis by ACBP, which we anticipate is of crucial importance in un

21 fected COS-7 cells significantly colocalized ACBP and HNF-4 alpha within the nucleus and in the perin

22 (v) Immunogold electron microscopy detected ACBP within 43 A of HNF-4 alpha.

23 n, however, is not affected by the disrupted ACBP gene.

24 lution crystal structures of a P. falciparum ACBP-acyl-CoA complex and of bovine ACBP in two crystal

25 chemical properties of Plasmodium falciparum ACBP are described together with the 2.0 A resolution cr

26 hat, even in the earliest stages of folding, ACBP dynamics are governed by native-like contacts on a

27 tivates CerS3 activity, whereas cytosol from ACBP knock-out mice does not.

28 mation was altered by oleoyl-CoA in the holo-ACBP as shown by a 2-A decrease of ACBP hydrodynamic dia

29 (i) ACBP interaction with HNF-4 alpha elicited significant c

30 (ii) ACBP and HNF-4 alpha were coimmunoprecipitated by antibo

31 yl-CoA-induced conformational alterations in ACBP may be significant to its putative functions in lip

32 HOKS in the preferred direction increased ACBP mRNA transcription and ACBP protein expression.

33 -binding protein/diazepam binding inhibitor (ACBP/DBI) motif.

34 Thus, native liver ACBP binds >14-carbon fatty acyl-CoAs with nanomolar aff

35 troscopy directly established that rat liver ACBP bound 18-carbon cis- and trans-parinaroyl-CoA, Kd =

36 Moreover, ACBP required the presence of the peripheral benzodiazep

37 P was engineered to contain the native mouse ACBP amino acid sequence expressed as a fusion protein a

38 test this hypothesis, mouse recombinant (mr) ACBP was engineered to contain the native mouse ACBP ami

39 ated that the expression of a Brassica napus ACBP (BnACBP) complementary DNA in the developing seeds

40 n tag resulted in mrACBP identical to native ACBP as shown by mass (10000.5 Da) and amino acid sequen

41 The mechanical behavior of ACBP is discussed in terms of topology and helix propens

42 on was confirmed by coimmunoprecipitation of ACBP and the alpha1-subunit of the GABA(A) receptor usin

43 the holo-ACBP as shown by a 2-A decrease of ACBP hydrodynamic diameter and increased Trp segmental m

44 Surprisingly, the deformability of ACBP is greater than that observed for the highly pliant

45 To address this issue, the effect of ACBP on liver LCFA-CoA pool size, acyl chain composition

46 onstraints for the acid-unfolded ensemble of ACBP.

47 gic transmission, HOKS-induced expression of ACBP could provide a molecular basis for adaptation to H

48 , smaller truncated proteolytic fragments of ACBP do, increasing the excitability of central cortical

49 chain fatty acyl-CoAs, direct interaction of ACBP with >14-carbon fatty acyl-CoAs has not been establ

50 experiments to study the folding kinetics of ACBP on the microsecond time scale.

51 een the level of luciferase and the level of ACBP expression.

52 transgenic mice expressing a higher level of ACBP.

53 induces the rapid release and processing of ACBP to the active peptides.

54 rapamycin also resulted in rapid release of ACBP indicating that this protein uses components of the

55 Overall, these data support the role of ACBP in acyl trafficking in developing seeds and validat

56 The functional significance of ACBP interaction with HNF-4 alpha was evidenced by mamma

57 irst demonstrate that the denatured state of ACBP at near-zero denaturant is unusually compact and en

58 e structures in this acid-denatured state of ACBP, we rationalize the effects of single-point mutatio

59 Suppression of ACBP levels by small interfering RNA inhibited the t-Bid

60 s are significantly reduced in the testes of ACBP(-/-) mice, concomitant with a significant reduction

61 y curved membranes to facilitate transfer of ACBP-bound ligands.

62 previously; however, the bovine and parasite ACBP structures are less similar.

63 The parasite ACBP is shown to have a different ligand-binding pocket,

64 dentified between the mammalian and parasite ACBPs.

65 yeast suggest that acyl-CoA binding protein ACBP may modulate long-chain fatty acyl-CoA (LCFA-CoA) d

66 id-denatured state of the 86-residue protein ACBP.

67 binding proteins, acyl CoA binding protein (ACBP) and sterol carrier protein-2 (SCP-2), by 45 and 80

68 study, we identify acyl-CoA-binding protein (ACBP) as playing a critical role in the activation of ca

69 ndle protein acyl co-enzyme binding protein (ACBP) as seen from both perturbations in nuclear magneti

70 (CI2) and unfolded acyl-CoA binding protein (ACBP) even under conditions where folded and unfolded su

71 Acyl-CoA-binding protein (ACBP) functions both intracellularly as part of fatty ac

72 ) cytosolic acyl-coenzyme A-binding protein (ACBP) has a substantial influence over fatty acid (FA) c

73 Although acyl-CoA-binding protein (ACBP) has been detected in the nucleus, the physiologica

74 ough the cytosolic acyl-CoA binding protein (ACBP) has high affinity for medium chain fatty acyl-CoAs

75 e four-alpha-helix acyl-CoA binding protein (ACBP) in the low-force regime using optical tweezers and

76 Acyl-CoA binding protein (ACBP) maintains a pool of fatty acyl-CoA molecules in th

77 Acyl-CoA-binding protein (ACBP) mRNA was one of four mRNAs selected by this screen

78 Acyl-CoA binding protein (ACBP) plays a role in determining the level of FAR eleme

79 e show that acyl-coenzyme A-binding protein (ACBP) potently facilitates very-long acyl chain ceramide

80 Although acyl-CoA binding protein (ACBP) stimulates utilization of long-chain fatty acyl-Co

81 on study of acyl-coenzyme A binding protein (ACBP), a two-state folder (folding time ~10 ms) exhibiti

82 Acyl coenzyme A (CoA)-binding protein (ACBP; AcbA in Dictyostelium discoideum), an unconvention

83 n experimentally well-characterized protein, ACBP, to explore the extent to which state-of-the-art si

84 ing affinity exhibited by murine recombinant ACBP: saturated > monounsaturated > polyunsaturated C14-

85 Muller cells may secrete ACBP in the inner plexiform layer, thereby decreasing th

86 Following secretion, ACBP is proteolytically cleaved to the active neuropepti

87 These interactions were specific since ACBP did not interact with Sp1 or glucocorticoid recepto

88 In summary, ACBP physically interacted with HNF-4 alpha in vitro and

89 "pull-down" assay in which histidine-tagged ACBP was used to pull down the GABA(A)alpha1.

90 when manipulated from the N- and C-termini, ACBP unfolds by populating a transition state that resem

91 e first time in a physiological context that ACBP expression may play a role in LCFA-CoA metabolism.

92 Time-resolved fluorometry revealed that ACBP-bound parinaroyl-CoAs had high rotational freedom w

93 Importantly, we show that ACBP interacts with CerS2 and CerS3.

94 Five lines of evidence showed that ACBP bound HNF-4 alpha in vitro and in the nucleus of in

95 A Markov state model (MSM) of the ACBP folding reaction, constructed from over 30 ms of mo

96 Disruption of the ACBP gene causes a >5-fold activation of OLE1 transcript

97 These effects were not due to the ACBP transgene altering the protein levels of liver micr

98 As shown herein for the first time, ACBP in the nucleus physically and functionally interact

99 brane-bound enzymes, it is not known whether ACBP directly interacts with membranes.

100 eripheral benzodiazepine receptor (for which ACBP is a ligand) to be retained at the mitochondria, to

101 While ACBP transgenic mice did not exhibit altered body or liv