ライフサイエンスコーパス: AFLP

1 AFLP analysis also disclosed that the same AFLP restrict

2 AFLP analysis provided higher resolution and could be us

3 AFLP analysis revealed a high degree of genomic polymorp

4 AFLP analysis using a selective primer delineated five p

5 AFLP and PFGE showed that the isolates from humans and c

6 AFLP fingerprinting revealed a similar pattern, with mos

7 AFLP identified a large cluster of 31 indistinguishable

8 AFLP is a reliable molecular technique that has provided

9 AFLP marker analyses revealed the pattern and extent of

10 AFLP marker genotyping, using the EcoRI and TaqI restric

11 AFLP markers are extremely sensitive to even small seque

12 AFLP revealed significant intraspecific genetic variatio

13 AFLP tradeoffs may differ among studies, but our results

14 AFLP was rapid, fairly inexpensive, and reproducible and

15 AFLP-, RAPD-, and RFLP-derived markers were used to satu

16 ns in their chloroplast haplotype and for 10 AFLP markers.

17 A total of 11 AFLP-derived SCAR markers, previously tagged to the Vf l

18 an F(2) mapping population (N = 539) at 154 AFLP, microsatellite, and gene-based markers.

19 er size classes were also genotyped with 155 AFLP loci.

20 The map was made with 172 AFLP and 10 anonymous codominant markers segregating amo

21 Allele frequencies of 18 AFLP markers (64.3%) for Mbita and of 26 markers (92.9%)

22 imer combination generated an average of 8.2 AFLP markers eligible for linkage mapping.

23 sis and the method and a case study with 201 AFLP and SSR markers scored on 228 full-sib individuals

24 Sequencing of 24 AFLP-cDNA fragments revealed genes with a variety of fun

25 an F(2) mapping population (N = 526) at 255 AFLP, microsatellite, and gene-based markers and derived

26 rised of 972 molecular markers (538 Bin, 258 AFLPs, 175 SSRs, and an EST).

27 ere, we genotype 23 pinniped species for 310 AFLP markers and find a strong phylogenetic signal, with

28 A molecular map with 102 RFLP, 34 AFLP and six microsatellite markers has been used to map

29 This genome-wide analysis of 357 AFLP characters (polymorphic fragments) indicates that B

30 (DHLs) that were genotyped for 368 RFLP, 358 AFLP, and 3 isozyme markers.

31 neybee genome were constructed from over 400 AFLP markers.

32 A total of 418 AFLP markers were assigned to 44 linkage groups.

33 The 418 AFLP markers covered 1593 cM Kosambi.

34 lysis resulted in a dense linkage map of 541 AFLP and 111 SSR markers distributed across 19 linkage g

35 A total of 607 AFLP markers were analyzed using 65 primer combinations

36 imates of f, along with genetic data from 94 AFLP markers and 12 microsatellites.

37 Based on the presence/absence of amplified AFLP fragments and pairwise similarity values, all the S

38 To further test that possibility, an AFLP-based genetic map of the Hessian fly genome was con

39 etraploid cotton, of genotype AAGG, using an AFLP-cDNA display screen.

40 predominant MPIL genotype (genotype A18) and AFLP genotypes (genotypes Z1 and Z2) of M. paratuberculo

41 Thus, the combination of fingerprinting and AFLP-based contig assembly and mapping provides a reliab

42 REA, PFGE, slpAST, PCR-ribotyping, MLST, and AFLP.

43 each of these 11 pairs by both RAPD-PCR and AFLP analyses.

44 Both rep-PCR and AFLP assays were rapid and reproducible, with high indic

45 The changes detected by RAPD-PCR and AFLP indicate that genetic drift occurs within H. pylori

46 s of our study indicate that the rep-PCR and AFLP techniques enable rapid fingerprinting of P. multoc

47 Concordance analyses of rep-PCR and AFLP with somatic serotyping indicate that, in general,

48 PFGE and AFLP were less discriminatory than ribotyping and RAPD.

49 n, noninbred parents was scored for RAPD and AFLP markers, in order to develop a linkage map.

50 We propose that RAPD and AFLP provide valuable tools for molecular typing of M. b

51 chosen and characterized by PFGE, RAPD, and AFLP.

52 and subjected to analysis by PFGE, RAPD, and AFLP.

53 es were undertaken using plastid regions and AFLP fragments.

54 be expected to complement existing RFLP and AFLP maps, increasing the power and resolution of genome

55 -83 (low-ABA) which was mapped with RFLP and AFLP markers.

56 lated data set and to a small set of SSR and AFLP markers scored in a full-sib population of tetraplo

57 Integrating the SSR- and AFLP-based maps generated 31 linkage groups comprising 1

58 sing data from GIS, morphological traits and AFLP markers.

59 Molecular markers Xbarc113 and AFLP AGCTCG-347 on chromosome 6A, Xbarc121 and Xbarc49 o

60 Dominant markers (DArTs and AFLPs) are commonly used for genetic analysis in the fie

61 n ten linkage groups; 2454 of those loci are AFLP products generated from either the EcoRI/MseI or Ps

62 to DNA fingerprinting applications, such as AFLP.

63 g DNA polymorphisms (primarily identified as AFLPs) as molecular genetic markers.

64 The association between AFLP and the E474Q mutation in the fetus is significant.

65 Both AFLP and RAPD separated the isolates into two distinct g

66 Inbreeding estimates based on both AFLP and microsatellite markers were found to correlate

67 approximately 65% of the contigs aligned by AFLP analysis had sufficient overlap to be confirmed by

68 tion, 30% of the overlapping BACs aligned by AFLP analysis provided information for merging contigs a

69 this mutation in pregnancies complicated by AFLP could allow early diagnosis and treatment in newbor

70 n phylogenetic tree with clusters defined by AFLP.

71 argely agreed with other clusters defined by AFLP.

72 ina, microsatellite markers were obtained by AFLP of sequences containing repeats (FIASCO).

73 provided only 77% of the detail provided by AFLP analysis.

74 tuberculosis-polymorphic regions revealed by AFLP were cloned and sequenced.

75 in latex, we analyzed more than 20,000 cDNA-AFLP-based TDFs (transcription-derived fragments) and 11

76 o VPE was found to be up-regulated in a cDNA-AFLP analysis of host responses of a wild peanut, Arachi

77 ntal stressors, we used microarrays and cDNA-AFLP to identify Expressed Sequence Tag (EST) fragments

78 differential display techniques such as cDNA-AFLP (Amplification Fragment Length Polymorphism).

79 at are used extensively by the authors, cDNA-AFLP and cDNA microarraying.

80 Expression was determined by cDNA-AFLP coupled with silver staining of the gels.

81 and tetraploid (T. turgidum) parents by cDNA-AFLP display.

82 mplified fragment length polymorphisms (cDNA-AFLP), to systematically characterize hundreds of the ge

83 procal formation, comprised 0.6% of the cDNA-AFLP bands.

84 Most of the cDNA-AFLP polymorphisms apparently resulted from loss of pare

85 carried out with seven to confirm their cDNA-AFLP differential pattern.

86 protein to follow infection over time, cDNA-AFLP to find genes up-regulated during virus infection i

87 Using cDNA-AFLPs followed by genomic and cDNA cleaved amplified pol

88 Data from 32 different AFLP primer combinations identified approximately 2400 B

89 methods such as SAGE, Differential Display, AFLP, etc. which rely on PCR.

90 Analysis using Taxotron revealed 10 distinct AFLP profiles among 57 isolates.

91 Thirty-eight AFLP primer combinations provided 585 informative marker

92 Eighteen AFLP primer combinations were used to genotype two recip

93 Every AFLP study initially generates markers with a range of l

94 lymyxa, and B. stearothermophilus shared few AFLP markers with B. anthracis and were used as outgroup

95 satellites and 200 individuals, 100 loci for AFLPs.

96 satellites and 150 individuals, 100 loci for AFLPs.

97 lolly pine (Pinus taeda L.), using data from AFLP markers from an essentially complete genome map.

98 Together with DNA fingerprints derived from AFLP markers, we evaluated variation in fruit and plant

99 mated from mitochondrial DNA with those from AFLPs and contrast traditional PAUP(*) Nei-Li AFLP genet

100 EcoRI/PstI-digested genomic DNA to generate AFLP bands and identified 309 markers that segregated am

101 Nine primer pairs were used to generate AFLP patterns, with a total number of amplified fragment

102 murium var. Copenhagen strains) but that had AFLP DNA fingerprints similar or identical to those of s

103 Twenty-seven women had AFLP and 81 had HELLP syndrome.

104 Here, AFLP bulk segregant analysis using H. melpomene crosses

105 However, AFLP markers did establish the presence or absence of th

106 However, Clustal DNA alignments identified AFLP restriction enzyme sites that were undigested in th

107 In AFLP analysis, bacterial genomic DNA is digested with re

108 xperimental climate treatments at individual AFLP loci in P. lanceolata, but not in F. ovina.

109 FLPs and contrast traditional PAUP(*) Nei-Li AFLP genetic distances with a recently proposed method u

110 regating for api and have generated X-linked AFLP markers.

111 (BAC) clones identified with the ASGR-linked AFLPs or previously mapped markers, when mapped by fluor

112 R, we have identified eight new ASGR-linked, AFLP-based molecular markers, only one of which showed r

113 A total of 347 loci (AFLPs, RAPDs, and protein-coding loci) were mapped using

114 oci are scored (eg 250 loci, typical of many AFLP studies).

115 Chromosome numbers and molecular markers (AFLPs) document the inheritance of the maternal genome t

116 , 4x-2) F1s, three lack a subset of maternal AFLP markers.

117 Using a modified AFLP technique called transposon display, 215 elements w

118 sm (AFLP) and methylation sensitive-AFLP (MS-AFLP) markers, we tested the hypothesis that differentia

119 er, crowding for a day resulted in neural MS-AFLP fingerprints that were clearly distinct from both c

120 ed locusts show markedly different neural MS-AFLP fingerprints.

121 amplified fragment length polymorphisms (MS-AFLP) to compare the effect of acute and chronic crowdin

122 Some clustering of the EcoRI/MseI AFLP markers was observed, possibly in centromeric regio

123 ground differentiation at putatively neutral AFLP loci was close to zero, and genomewide genetic stru

124 Among the non-AFLP markers, 136 are SSRs previously mapped in sorghum,

125 Cluster analysis of AFLP marker scores identified 10 groups of animals with

126 Application of AFLP highlighted the genetic homogeneity of Brucella.

127 Using a linkage map constructed of AFLP and microsatellite markers, three QTL controlling b

128 In spite of this determination of AFLP profiles of large numbers of isolates of human and

129 in 5 families (19%) with maternal history of AFLP (95% confidence interval, 9%-54%).

130 Homology of AFLP patterns of 35% or less was used as a cutoff value

131 oroplast haplotype but showed high levels of AFLP genotypic diversity consistent with sexual reproduc

132 tantially add to the discriminatory power of AFLP when applied to Brucella and enhance the value of t

133 ers coupled with computational prediction of AFLP loci has enabled its correspondence with the newly

134 i isolates compared well with the results of AFLP.

135 genetic loci and the pan-genomic sampling of AFLP appears to offer a well-supported assessment of B.

136 On average, each set of AFLP primers amplified 28 single-copy DNA markers that w

137 FLP) markers revealed that a small subset of AFLP markers showed 'outlier' genetic differentiation am

138 These data further emphasize the utility of AFLP markers as general tools for phylogenetic reconstru

139 lone (P = 0.320), demonstrating the value of AFLP and RAPD analyses in the characterization of diseas

140 Based on AFLP marker similarity, the ongoing anthrax epidemic in

141 ch less informative markers, such as SNPs or AFLPs, can reach the same power for parentage and sibshi

142 and the population genetic structure (in our AFLP dataset) was partly explained by isolation by envir

143 In addition, we confirmed X linkage of our AFLP markers and api by using one X-linked marker develo

144 PFGE, AFLP, and MLVA grouped two, one, and two different non-A

145 Plotting AFLP divergence time estimates against those based on bo

146 d gametophytes, genotyped at 121 polymorphic AFLP loci, three gene-based nuclear loci, one chloroplas

147 d in 46 linkage groups, and 1574 polymorphic AFLP markers were identified.

148 per line were genotyped for 239 polymorphic AFLP markers.

149 Amplified fragment length polymorphic (AFLP) markers were used to discriminate between lines of

150 Amplified restriction fragment polymorphism (AFLP) is a PCR-based DNA fingerprinting technique.

151 and amplified fragment length polymorphism (AFLP) analyses were used to fingerprint M. avium subsp.

152 d by amplified fragment length polymorphism (AFLP) analysis and by sequencing of the 16S rRNA gene an

153 cDNA-amplified fragment length polymorphism (AFLP) analysis of leaves from mature plants revealed tha

154 Amplified fragment-length polymorphism (AFLP) analysis of the isolates was combined with epidemi

155 s by amplified fragment length polymorphism (AFLP) analysis, which revealed DNA fingerprint similarit

156 d by amplified fragment length polymorphism (AFLP) analysis.

157 and amplified fragment length polymorphism (AFLP) analysis; and ELISA, to determine Lewis phenotypes

158 s of amplified fragment length polymorphism (AFLP) and bulk segregant analysis were used to map the D

159 sing Amplified Fragment Length Polymorphism (AFLP) and methylation sensitive-AFLP (MS-AFLP) markers,

160 with amplified fragment length polymorphism (AFLP) and microsatellite markers) were correlated with t

161 148 amplified fragment length polymorphism (AFLP) and six single-strand conformation polymorphism (S

162 use amplified fragment length polymorphism (AFLP) data to assess genetic divergence and test for sig

163 and amplified fragment length polymorphism (AFLP) fingerprinting.

164 d by amplified fragment length polymorphism (AFLP) genomic fingerprinting to each other and to strain

165 Amplified fragment length polymorphism (AFLP) is a whole-genome fingerprinting method that relie

166 , an amplified fragment length polymorphism (AFLP) is heterozygous in males and homozygous recessive

167 With amplified fragment-length polymorphism (AFLP) markers and controlled crosses we constructed link

168 sing amplified fragment length polymorphism (AFLP) markers and randomly amplified polymorphic DNA (RA

169 phic Amplified Fragment Length Polymorphism (AFLP) markers as a means to assess small-scale genetic h

170 sing amplified fragment length polymorphism (AFLP) markers revealed that a small subset of AFLP marke

171 sing amplified fragment length polymorphism (AFLP) markers revealed three QTL for beetle resistance t

172 1498 amplified fragment length polymorphism (AFLP) markers, the papaya ringspot virus coat protein ma

173 sing amplified fragment length polymorphism (AFLP) markers.

174 the amplified fragment length polymorphism (AFLP) method.

175 cDNA-amplified fragment length polymorphism (AFLP) pattern of Agrobacterium- and mock-inoculated Ager

176 ible amplified fragment length polymorphism (AFLP) profiles, but was unsuccessful with 13 other speci

177 sing amplified fragment length polymorphism (AFLP) technology, resolved on a LI-COR dual-dye DNA sequ

178 for amplified fragment length polymorphism (AFLP) to characterize the genomes of 20 Mycobacterium av

179 e of amplified fragment length polymorphism (AFLP) to develop a linkage map of Laupala.

180 ) or amplified fragment length polymorphism (AFLP) types.

181 Amplified fragment length polymorphism (AFLP) was employed as a genetic analysis tool for the st

182 Amplified fragment length polymorphism (AFLP) was investigated for the differentiation of Coryne

183 Amplified fragment length polymorphism (AFLP) was used for typing avian mycoplasma species.

184 and amplified fragment length polymorphism (AFLP) were used to characterize a sample of 43 field iso

185 nes, amplified fragment length polymorphism (AFLP), and pulsed-field gel electrophoresis (PFGE).

186 GE), amplified fragment length polymorphism (AFLP), and random amplified polymorphic DNA (RAPD) analy

187 VA), amplified fragment length polymorphism (AFLP), surface layer protein A gene sequence typing (slp

188 amplification fragment length polymorphism (AFLP), these strains were grouped into three different c

189 e of amplified fragment length polymorphism (AFLP), whereby we visually assessed the agreement of the

190 pply amplified fragment length polymorphism (AFLP)-cDNA display to perform a genome-wide screen for o

191 sing amplified fragment length polymorphism (AFLP).

192 and amplified fragment length polymorphism [AFLP]) for the characterization of C. diphtheriae strain

193 by amplified fragment length polymorphisms (AFLP) and multilocus sequence typing (MLST).

194 rs [amplified fragment length polymorphisms (AFLP) and simple sequence repeats (SSR)] we have generat

195 ied amplified fragment length polymorphisms (AFLP) cosegregating with fusibility, used these markers

196 on amplified fragment length polymorphisms (AFLPs) and genes, consists of 31 linkage groups (LGs; co

197 ith amplified fragment length polymorphisms (AFLPs) and microsatellite markers.

198 as amplified fragment length polymorphisms (AFLPs) and randomly amplified polymorphic DNA (RAPDs), t

199 Amplified fragment length polymorphisms (AFLPs) are widely used for phylogenetic reconstruction i

200 of amplified fragment length polymorphisms (AFLPs) for resolving deep evolutionary relationships.

201 of amplified fragment length polymorphisms (AFLPs) in G. laevigata, focusing on individuals from the

202 Amplified fragment length polymorphisms (AFLPs) provide a rapid and inexpensive source of multilo

203 ing Amplified Fragment Length Polymorphisms (AFLPs) revealed no genetic differences between the two s

204 112 anonymous fragment length polymorphisms (AFLPs) was studied in 41 haploid embryos derived from a

205 and amplified fragment length polymorphisms (AFLPs).

206 for amplified fragment length polymorphisms (AFLPs).

207 ing amplified fragment length polymorphisms (AFLPs).

208 Acute fatty liver of pregnancy (AFLP) and hemolysis, elevated liver enzymes, and low pla

209 Acute fatty liver of pregnancy (AFLP) and the syndrome of hemolysis, elevated liver enzy

210 lectrophoretic karyotypes) and PCR products (AFLP procedures) were determined for Microbotryum lineag

211 trast to the variation among taxa, only rare AFLP marker variation was observed within B. anthracis,

212 A BAC identified with the recombinant AFLP marker mapped most proximal to the centromere of th

213 Data from RFLP, AFLP, and microsatellite analysis were used to create a

214 mission ratio distortion (TRD) of 729 RFLPs, AFLPs, and isozyme markers distributed across the genome

215 ust/soil, honey) usually located to the same AFLP clade as the patient's isolate, thereby identifying

216 AFLP analysis also disclosed that the same AFLP restriction sites were digested in all of the fecal

217 and environmental strains that had the same AFLP/MLST genotypes.

218 We used 34 pairs of two-base selective AFLP primers and identified 1048 polymorphic markers tha

219 olymorphism (AFLP) and methylation sensitive-AFLP (MS-AFLP) markers, we tested the hypothesis that di

220 omosomal region encompassing api using seven AFLP markers.

221 ble maternal and perinatal outcome in severe AFLP and HELLP syndrome.

222 their ease of amplification in any species, AFLP markers may prove to be a valuable new tool for est

223 We used male-specific AFLP markers to characterize the extent of deletions in

224 A genetic linkage map composed of 841 SSR, AFLP, and RAPD markers and phenotypic data from 310 prog

225 g apple scab resistance gene(s) by targeting AFLP-derived SCAR markers to this specific genomic regio

226 A total of 1354 testcross AFLP marker loci were evaluated in the three parental ma

227 t by the RFLP/SSR markers demonstrating that AFLP technology is effective in providing excellent geno

228 ha and DH5 alpha F'IQ strains indicated that AFLP is able to resolve differences in F' episomal DNA (

229 Here we show that AFLPs provide resolution of deep relationships within th

230 The AFLP data reveal unprecedented resolution, clustering sa

231 The AFLP dendrogram, the largest to date, contained 154 clad

232 The AFLP markers showed a high level of clustering that appe

233 In general, however, the AFLP markers filled most of the gaps left by the RFLP/SS

234 alysis identified linear combinations of the AFLP marker scores that grouped animals by selection lin

235 apping deletions to predict the order of the AFLP markers and to locate the mutated sex-determining g

236 To this end, a modification of the AFLP procedure called transposon display was used to gen

237 sufficient to overcome this obstacle of the AFLP technique for deep divergences.

238 95% of the microsatellites and 92% of the AFLPs were linked in the final map.

239 Statistical tests indicate that these AFLP markers tend to cluster over the map, with the coef

240 This AFLP map provides a framework for locating genes on the

241 mic differences were detected, and thus this AFLP approach is likely to prove most useful for identif

242 eight distribution pattern was compatible to AFLP-based clustering analysis for the collection.

243 (n = 131) and VNTRs (n = 31), in addition to AFLPs (n = 66) and 7 other markers.

244 e effect of crossing parental lines from two AFLP-based groups on carotenoid accumulation and agronom

245 One hundred and twenty-two AFLP markers were mapped using an IR64 x Azucena rice do

246 We have used AFLP (amplified fragment length polymorphism) DNA marker

247 Here, we used AFLPs to compare population structure in co-distributed

248 Using AFLP markers, we constructed the first linkage map of Co

249 Using AFLP technology and a recombinant inbred line population

250 a detailed genetic linkage map of ECB using AFLP and microsatellite markers and map the factors resp

251 e bands in a denaturing sequencing gel using AFLP.

252 Molecular marker analysis using AFLPs and isoenzymes did not identify any clear major ge

253 minal bleeding occurred in 12 women (10 with AFLP), 9 of whom required laparotomies for clot evacuati

254 atures and laboratory values consistent with AFLP, and 7 (15%) had HELLP syndrome.

255 Four patients with AFLP (12.5%) had a fatal outcome, with a corresponding p

256 ications occurred in 17 of the patients with AFLP (53%) and in 2 of those with HELLP syndrome (29%).

257 ing a linkage map constructed primarily with AFLP markers, QTL mapping was performed, including MCE c