ライフサイエンスコーパス: APC2

1 gene we have identified by this means, RSI1/APC2.

2 strate recognition--and the cullin domain of Apc2.

3 ibution of Apc1 is much greater than that of Apc2.

4 We isolated six new alleles of Drosophila APC2.

5 with the anaphase promoting complex protein Apc2.

6 However, the minimal APC2/11 ubiquitin ligase module does not possess substra

7 e treatment also perturbed the enrichment of Apc2, a component of the centrosome-anchoring machinery,

8 lins while APC11 specifically interacts with APC2, a cullin-related APC subunit.

9 reduced only anterograde movements, whereas APC2 (aa 1034-2130) or APC3 (aa 2130-2844) reduced both

10 e in situ hybridization analysis revealed an APC2 allelic imbalance in 19 of 20 ovarian cancers scree

11 eveal that the combined activity of Apc1 and Apc2 allows a tight regulation of transcriptional activa

12 In addition they accumulate with APC2 and APC1 in nerves formed by axons of the progeny o

13 APC2 and APC1 localize to very different places when exp

14 ction was dependent on APC1, suggesting that APC2 and APC1 may act cooperatively in the destruction c

15 generated ovaries and embryos null for both APC2 and APC1, and assessed the consequences of total lo

16 h Ubc4 or UbcH10, a heterodimeric complex of APC2 and APC11 is sufficient to catalyze the ubiquitinat

17 g the cullin domain and RING finger subunits Apc2 and Apc11, respectively, and a tetratricopeptide re

18 the requirement for nuclear localization of APC2 and Axin in the Wg signal transduction pathway duri

19 APC2 and cullins may be distantly related members of a u

20 furrow extension, our analysis suggests that APC2 and DIA function in a novel complex that affects ac

21 rtebrate APC and DIA1, may not function with APC2 and DIA in furrow extension.

22 Colocalization of APC2 and DIA peaks during furrow extension, and localiza

23 c hypomorphs, suggesting an additive role of APC2 and F-actin in maintaining the adherens junctions t

24 alytic core contains the cullin-like subunit APC2 and its RING partner APC11, which collaborates with

25 hese domains are regulated, using Drosophila APC2 and its two SAMP repeats as our model.

26 ctly mediates self-association of Drosophila APC2 and plays an essential role in the assembly and sta

27 normal lungs, including the tumor suppressor APC2 and the oncogene Ros 1.

28 polarity factor aPKC, the junctional protein APC2, and basal integrins in epithelial spindle orientat

29 gulates a complex containing Zw3, Armadillo, APC2, and EB1 and that this complex has a role in stabil

30 inds a C-terminal RPQPSG motif in Drosophila APC2, and that this motif is conserved in human APC2, bu

31 s of APC in a model epithelium, we generated APC2 APC1 double null clones in the Drosophila wing imag

32 o regulate Wnt signaling in APC2 null and in APC2 APC1 double-null embryos.

33 catalytic and substrate recognition module (Apc2, Apc11 and Apc10 (also known as Doc1)), and TPR-pho

34 Seven core subunit homologs of APC/C (APC1, APC2, APC11, CDC16, CDC23, CDC27, and DOC1) were identif

35 The remaining four human APC subunits, APC2, APC4, APC5, and APC7, as well as human CDC23, were

36 adenomatous polyposis coli (APC)-like gene, APC2/APCL, was recently described and localized to chrom

37 The two Drosophila homologs of APC (Apc1 and Apc2) appear to have predominantly different tissue dist

38 I find that nuclear localization of APC2 appears to be required, but Axin can block signalin

39 The Apc2-Armadillo complex appears to link cap expansion to

40 Furthermore, APC2 binds DIA directly through a region of APC2 not pre

41 2, and that this motif is conserved in human APC2, but not human APC1.

42 directly PARylates both Drosophila Axin and APC2, but that PARylation does not globally regulate APC

43 In addition, we find that APC2 can recruit TNKS into the beta-catenin destruction

44 We show that loss of Drosophila Apc2 causes protein isoform changes reflecting misregula

45 no similarity to proteins of known function, APC2 contains a region that is similar to a sequence in

46 d genomic stability, e.g., hBUB3, hZW10, and APC2, contribute to the molecular pathogenesis and tumor

47 ovarian cancers screened and indicates that APC2 could be a potential tumor suppressor gene in ovari

48 wo separable subcomplexes, one that contains Apc2 (Cullin), Apc11 (RING), and Doc1/Apc10, and another

49 n in total Apc activity, achieved by loss of Apc2, decreases the effective threshold at which Wg elic

50 Thus, an APC2-DIA complex appears crucial for actin furrow extens

51 ho family GTPases, our data suggest that the APC2-DIA complex might be independent of RHOGEF2 and RHO

52 t that Drosophila syncytial embryos null for Apc2 display defects in the formation and extension of p

53 These data suggest that APC2 does not have to shuttle into the nucleus or locali

54 matosis polyposis coli (APC)-related protein APC2/E-APC and the MT + Tip protein EB1.

55 an cancer cells, which express low levels of APC2, exogenous APC2 localized to the Golgi apparatus, a

56 used mouse transgenics to delete Apc and/or Apc2 from mouse mammary epithelium to elucidate the sign

57 mechanism of action of APC2, revealing that APC2 functions at the embryonic cortex with several prot

58 n pattern was observed for TrkC-miR2 and the APC2 gene in colorectal cancer specimens.

59 The APC2 gene is essential in Saccharomyces cerevisiae, and

60 PCR and luciferase assays indicated that the APC2 gene is targeted by TrkC-miR2, and Wnt signaling is

61 ein-protein interactions and localization of Apc2-GFP and Apc-RFP to branch points suggests that thes

62 are not known for folate biology, including APC2, GRM8, SLC16A12, OPCML, PRPH, LHX1, KLK4 and PRSS21

63 We show that axin, APC2, GSK-3beta and N-terminally phosphorylated forms of

64 APC2 has a highly dynamic, asymmetric localization throu

65 t, simultaneously inactivating both APC1 and APC2 in clones of cells in the Drosophila larval optic l

66 d levels of Akt result in mislocalization of APC2 in postcellularized embryonic mitoses and misorient

67 roteins adenomatous polyposis coli (APC) and APC2 in the pathogenesis of human breast cancer are ill-

68 t the formin Diaphanous (DIA) functions with APC2 in this process.

69 -conserved D-box residues abrogates inversin-Apc2 interaction.

70 aken together, our results suggest that RSI1/APC2 is a subunit of APC.

71 terminus of human APC2 show that endogenous APC2 is diffusely distributed in the cytoplasm and coloc

72 These results suggest that APC2 is involved in actin-associated events and could in

73 which express low levels of APC2, exogenous APC2 localized to the Golgi apparatus, actin-containing

74 t places when expressed in the larval brain: APC2 localizes to the cell cortex and APC1 to centrosome

75 Our findings suggest that APC2 loss leads to increased rates of chromosome segrega

76 radation, and cell signaling are affected by Apc2 loss.

77 , reduction of dia enhances actin defects in Apc2 mutant embryos.

78 s essential in Saccharomyces cerevisiae, and apc2 mutants arrest at metaphase and are defective in th

79 Temperature-sensitive rsi1/apc2 mutants arrest in metaphase and are unable to degra

80 In addition, like our rsi1/apc2 mutations, cdc23-1, encoding a known APC subunit, i

81 We find that both Apc1 and Apc2 negatively regulate Arm activity in photoreceptors,

82 APC2 binds DIA directly through a region of APC2 not previously shown to interact with DIA-related f

83 f these mutants to regulate Wnt signaling in APC2 null and in APC2 APC1 double-null embryos.

84 ll lines stably transfected with LEF1(DN) or APC2, or transiently transfected with short-interfering

85 We find that APC1 and APC2 play overlapping roles in regulating Wingless signa

86 Our in vivo data demonstrate that APC2 protects genome stability by modulating mitotic fid

87 t that PARylation does not globally regulate APC2 protein levels as it does for Axin.

88 mplex activity at the level of both Axin and APC2, providing further mechanistic insight into TNKS in

89 APC2 remained associated with actin filaments after trea

90 rexpression of Adenomatous Polyposis Coli 2 (APC2) rescued GSC loss in chic hypomorphs, suggesting an

91 risingly, although complete loss of APC1 and APC2 resulted in strong activation of Wingless signaling

92 theses related to the mechanism of action of APC2, revealing that APC2 functions at the embryonic cor

93 rylation and extended region interactions in APC2's destruction complex function, but suggest that th

94 Antibodies against the NH2 terminus of human APC2 show that endogenous APC2 is diffusely distributed

95 We show mr encodes the APC2 subunit of the anaphase promoting complex/cyclosome

96 as a putative binding partner of Drosophila APC2, suggesting that TNKS may play multiple roles in de

97 As expected of an Apc2 target, inversin possesses D-boxes and site-directe

98 Indeed, the anaphase block in rsi1/apc2 temperature-sensitive mutants is overcome by remova

99 argeted mutations in Drosophila melanogaster APC2 that disrupt phosphorylation and extended region in

100 eta-catenin destruction complex, placing the APC2/TNKS interaction at the correct intracellular locat

101 We have fine mapped APC2 to a small region of chromosome 19p13.3 containing

102 during furrow extension, and localization of APC2 to furrows is DIA-dependent.

103 th localizes the microtubule binding protein Apc2 to orient one GSC centrosome at the niche-GSC inter

104 Inhibition of an APC subunit (APC2) using short interfering RNA knockdown impaired s80

105 estruction complex by generating a series of APC2 variants to which we added tags relocalizing otherw

106 to the C-terminal cullin homology domain of APC2, whereas Ubc4 interacts with APC11 directly.

107 UBCH10 is corecruited via interactions with APC2, which we visualized in a trapped complex represent

108 he Axin/Adenomatous polyposis coli (Apc1 and Apc2)/Zeste-white 3 destruction complex, and Wg-dependen