ライフサイエンスコーパス: AQP

1 AQP deletion did not significantly affect W/D at 45 min

2 AQP inhibition may thus reduce the metastatic potential

3 AQP-0 was located almost exclusively in DSMs at a 1:1200

4 AQP-1 enhances osmotic water permeability and FGF-induce

5 AQP-1 expression and localization was examined in normal

6 AQP-1 in young reticulocytes localizes to the plasma mem

7 AQP-1 levels were modulated in LEC using retroviral over

8 AQP-1 overexpression promotes fibroblast growth factor (

9 AQP-2 levels were lower during early postnatal life, rea

10 AQP-2 possesses a single consensus cAMP-dependent protei

11 AQP-2 urinary excretion changed with short-term alterati

12 the eye lens, the water channel aquaporin-0 (AQP-0) and the connexins Cx46 and Cx50, are preferential

13 hat RD alters the expression of aquaporin-0 (AQP-0), and this modulation is prevented by treatment wi

14 Aquaporin-1 (AQP-1), the universal water channel, is responsible for

15 cated almost exclusively in DSMs at a 1:1200 AQP-0/lipid ratio, whereas approximately 50% of the prot

16 downregulation of whole kidney aquaporin-2 (AQP-2) protein and mRNA expression.

17 ed insertion and removal of the aquaporin-2 (AQP-2) water channel.

18 Aquaporin-2 (AQP-2), a water channel located on the apical membrane o

19 To define the role of aquaporin-2 (AQP-2), the developmental expression of this water chann

20 pressin-sensitive water channel aquaporin-2 (AQP-2).

21 ressin-sensitive water channel (aquaporin 2; AQP-2) mediates water transport across the apical plasma

22 Additionally, AQP-1 localizes to plasma membrane blebs, where it incre

23 The GFP fusion did not affect AQP tetrameric association or water transport function.

24 om this study, it is concluded that although AQP-2 expression may play a role in the development of u

25 logy of hKID to human MIP (48% identity) and AQP-2 (52%), with lesser homology to human MIWC (AQP-4,

26 identical chromosomal loci of hKID, MIP, and AQP-2 suggest a MIP family gene cluster at chromosome lo

27 Among those genes, AMT, NRT and AQP for N uptake and GOGAT and GS for N assimilation wer

28 on, diffusional water permeability (Pd), and AQP expression.

29 t there were no differences in wild-type and AQP knockout mice.

30 omparably in mitochondria from wild-type and AQP-deficient mice, although the slowing was explained b

31 in low amounts, we hypothesized that another AQP must be expressed at this domain to facilitate trans

32 Aquaporin (AQP) 4 is the predominant water channel in the mammalian

33 Aquaporin (AQP) 6 belongs to the aquaporin water channel family.

34 Aquaporin (AQP) is a hexahelical integral membrane protein that fun

35 Aquaporin (AQP) water channel AQP3 has been proposed to be the majo

36 Aquaporin (AQP) water channel proteins are tetrameric assemblies of

37 Aquaporin (AQP) water channels are abundant in the brain and spinal

38 Aquaporin (AQP) water channels are expressed in high-grade tumor ce

39 Aquaporin (AQP) water channels are found throughout nature and conf

40 Aquaporin (AQP) water channels provide a major pathway for osmotica

41 Aquaporin (AQP) water-channel proteins are freely permeated by wate

42 Aquaporin (AQP)-1 is a hexahelical integral membrane protein that f

43 Aquaglyceroporin aquaporin (AQP)3 is the major glycerol channel in human and rat ery

44 colocalize with the water channel aquaporin (AQP) 4 in prominin-1 immunopositive (P(+)) precursors in

45 Water channel aquaporin (AQP)-4 is expressed in Muller cells in retina, which are

46 cking each of the water channels, aquaporin (AQP)-1, -3, and -5, normally expressed in cornea or conj

47 sed upstream and in-frame to each aquaporin (AQP).

48 Hypertonic aquaporin (AQP) induction has been described, but little is known a

49 cluded that amiloride-inhibitable aquaporin (AQP) water channels in airway epithelia modulate airway

50 how that the basolateral membrane aquaporin (AQP)-3, but not the equivalent apical membrane AQP5, is

51 We have cloned a novel aquaporin (AQP) from Xenopus laevis oocytes, which we have provisio

52 ough the insertion and removal of aquaporin (AQP) 2 water channels into the IMCD apical membrane.

53 results of a multicentre study of aquaporin (AQP) 4 antibody (AQP4-Ab) assays in neuromyelitis optica

54 can be used to study the roles of aquaporin (AQP) in cellular water permeability and screen AQP-speci

55 The function of aquaporin (AQP) protein in transporting water is crucial for plants

56 reports suggest the expression of aquaporin (AQP)-type water channels in mitochondria from liver (AQP

57 tion of the water channel protein aquaporin (AQP).

58 er of the major intrinsic protein/aquaporin (AQP) channel family, is a major component of the soybean

59 Immunocytochemistry showed strong aquaporin (AQP)-4 water channel expression in Muller cells in mouse

60 The aquaporin (AQP) family of integral membrane protein channels mediat

61 lar water flow is mediated by the aquaporin (AQP) family of membrane proteins.

62 emanating from the center of the aquaporin (AQP) water and solute channel is responsible for the rep

63 ulin 26 (NOD 26), a member of the aquaporin (AQP) water channel family, is a major protein component

64 Two aquaporin (AQP)-type water channels are expressed in mammalian corn

65 We show here that, in vitro, aquaporin (AQP) blockade or deficiency results in reduced IL-1beta

66 n might include interactions with aquaporin (AQP) water channel isoforms, although the proposed requi

67 by 56% to 58% by coinjection with aquaporin (AQP)5 antisense oligonucleotide.

68 Aquaporin- (AQP) 3, a water and glycerol channel, plays an important

69 Aquaporins (AQP) are members of the major intrinsic protein (MIP) su

70 Aquaporins (AQP) are water-specific membrane channel proteins.

71 f water channels, referred to as aquaporins (AQP): AQP0-AQP9.

72 es that cellular water channels, aquaporins (AQP), are central to both processes.

73 erol permeabilities of mammalian aquaporins (AQP) 1-5 and the major intrinsic protein of lens fiber (

74 ed previously that expression of aquaporins (AQP) 1, 4, and 5 in rat lung increased just after birth.

75 ember of the tonoplast family of aquaporins (AQP).

76 Aquaporins (AQPs) are biological water channels known for fast water

77 Aquaporins (AQPs) are channel proteins that regulate the movement of

78 Aquaporins (AQPs) are important in controlling water permeability.

79 Aquaporins (AQPs) are integral membrane proteins whose function is t

80 Aquaporins (AQPs) are integral membrane water channels, recognized f

81 Aquaporins (AQPs) are transmembrane water channels ubiquitously expr

82 Aquaporins (AQPs) are water channel proteins that are essential in b

83 Aquaporins (AQPs) are water channels allowing fast and passive diffu

84 Aquaporins (AQPs) in the major intrinsic family of proteins mediate

85 11 known channel proteins called aquaporins (AQPs) that are involved in transcellular water transport

86 lled by membrane proteins called aquaporins (AQPs).

87 tes express water channels (i.e. aquaporins (AQPs)), proteins that are increasingly recognized as imp

88 A role for aquaporins (AQPs) in hearing has been suggested from the specific ex

89 All characterized mammalian aquaporins (AQPs) are localized to plasma membranes where they funct

90 standing of the cellular role of aquaporins (AQPs) in the regulation of whole-plant hydraulics, in ge

91 and pathophysiological roles of aquaporins (AQPs), a family of water channel proteins, in the hepato

92 Compared to other aquaporins (AQPs), lens-specific AQP0 is a poor water channel, and i

93 While overexpression of several aquaporins (AQPs) has been reported in different types of human canc

94 The aquaporins (AQPs) are a family of water-transporting proteins that f

95 at 65 h (5.1) were significantly affected by AQP deletion.

96 amiloride, but clearance was not affected by AQP deletion.

97 ased from 3.7 to 7.5 but was not affected by AQP deletion.

98 by protein-lipid interactions as modified by AQP-0 homo-oligomerization.

99 he structural requirements rendering certain AQPs permeable for weak monoacids and the mechanism of c

100 of the mercurial-insensitive water channel (AQP-4) at the basolateral membrane of airway epithelial

101 value that is lower than other characterized AQPs.

102 homology to human MIWC (AQP-4, 34%), CHIP28 (AQP-1, 38%), and GLIP (AQP-3, 22%).

103 In conclusion, AQP-2 urinary excretion, as measured by quantitative Wes

104 Under normal conditions, AQP-2 levels in the immature rat were significantly lowe

105 Monocarboxylic acid-conducting AQPs thus employ a mechanism similar to the family of fo

106 g the seven human aquaporins cloned to date (AQPs 0-6), genes encoding the four most closely related

107 ver, direct functional studies demonstrating AQP-mediated water transport in cholangiocytes are limit

108 establish the structural basis for different AQP folding pathways and provide evidence that variation

109 Yet, data on D. discoideum AQPs is almost absent.

110 espite cloning of two putative D. discoideum AQPs, WacA, and AqpA, water permeability has not been sh

111 Thus, glucose-driven, AQP-mediated localized water influx is involved in the m

112 tion assays demonstrated that the endogenous AQP-2 promoter was occupied by TAp73 in a developmentall

113 ic fibrosis (non-CF) or CF airway epithelia, AQP-transfected Fisher rat thyroid cells, or intact lung

114 with AQP3- or AQP4-encoding genes to express AQPs in plasma membranes.

115 A sequences were designed to target all five AQP genes from the PLASMA MEMBRANE-INTRINSIC PROTEIN1 (P

116 Our results provide evidence for AQP-facilitated tumor cell migration and spread, suggest

117 and spread, suggesting a novel function for AQP expression in high-grade tumors.

118 time a physiologically significant role for AQP-0 in retinal function.

119 We identified and cloned four AQPs from C. lectularius, assessed tissue and lifestage-

120 ctivities that add and remove 32P label from AQP-2.

121 GFP-AQP lateral mobility was measured by irreversibly bleach

122 Using GFP-AQP fusion proteins expressed in HEK293 cells, we demons

123 (AQP-4, 34%), CHIP28 (AQP-1, 38%), and GLIP (AQP-3, 22%).

124 ion bears on the proposed mechanisms for how AQPs remain totally insulating to any proton conductance

125 Using an antibody directed to human AQP-2, a quantitative Western blot analysis was performe

126 bout the development of an antibody to human AQP-2, and measures the urinary excretion of AQP-2 by qu

127 To examine this hypothesis, AQP genes were silenced using artificial microRNAs that

128 To rule out a potential alteration in AQP-2 trafficking, the transport of this water channel w

129 Interestingly, the increase in AQP-2 observed in the immature kidney was not accompanie

130 cellular loop D domain, a region involved in AQP channel gating.

131 wild-type versus knock-out mice deficient in AQPs -1, -4, or -8.

132 and retrieval of "flux proteins", including AQPs, involved in canalicular bile secretion.

133 The hypothesis was tested that the increased AQP expression is associated with increased osmotic wate

134 Instead, AQP-2 phosphorylation by PKA may modulate AQP-2's distri

135 be correlated with downregulation of kidney AQP-2 expression during escape from antidiuresis.

136 fluid volume expansion, also regulate kidney AQP-2 expression in rats.

137 Whole kidney AQP-2 protein was measured by Western blotting, and inne

138 lial cells expressing individual airway/lung AQPs, and perfused mouse lung.

139 Similar to mammalian AQPs, water permeation of AgAQP1 is inhibited by HgCl(2)

140 sured by Western blotting, and inner medulla AQP-2 mRNA was determined by Northern blotting.

141 as a phenomenon in some plant and microbial AQPs.

142 2 (52%), with lesser homology to human MIWC (AQP-4, 34%), CHIP28 (AQP-1, 38%), and GLIP (AQP-3, 22%).

143 e mercurial insensitive water channel (MIWC, AQP-4) is a water-selective transporter expressed at the

144 d, AQP-2 phosphorylation by PKA may modulate AQP-2's distribution between plasma membrane and intrace

145 ndicate that nodulin 26 is a multifunctional AQP that confers water and glycerol transport to the SM,

146 ese novel observations suggest that multiple AQP expression may be advantageous to tumorigenesis, whi

147 We propose that AQPxlo is a new AQP paralogue unknown in mammals.

148 We cloned a novel AQP, AqpB, from amoeboidal D. discoideum cells.

149 The immune-related activity of AQP was confirmed in vivo in a model of acute lung infla

150 Mice lacking functional alleles of AQP-0 had a phototransduction deficit as assessed by ele

151 ole for PKA phosphorylation in alteration of AQP-2's Pf.

152 d immunizing peptide to derive the amount of AQP-2 contained in aliquots of urine.

153 cally examined the functional consequence of AQP expression in mitochondria by measurement of water a

154 Finally, we discuss how the discovery of AQP activators and inhibitors will be the next major ste

155 omains can play a role in the disposition of AQP-0 and the connexins in the plane of the membrane.

156 osmolalities, significant downregulation of AQP-2 expression compared to dDAVP-infused control rats

157 olality contributes to the downregulation of AQP-2 expression in this model.

158 AQP-2, and measures the urinary excretion of AQP-2 by quantitative Western analysis.

159 Steady-state 24-h urinary excretion of AQP-2 was 43 +/- 10 nmol/24 h (or 28.5 +/- 6.9 pmol/mg c

160 Our findings reveal a new function of AQP in the inflammatory process and suggest a novel ther

161 Inhibition of AQP specifically during the regulatory volume decrease p

162 ished high dDAVP-stimulated kidney levels of AQP-2 after 4 d of water restriction.

163 yers showed that the microdomain location of AQP-0 depended on protein/lipid ratio.

164 ssociated with osmotic water permeability of AQP-expressing cells and a slow phase time constant asso

165 To test whether PKA phosphorylation of AQP-2 alters channel Pf, we compared the Pf values of pu

166 uring maturation a part of the total pool of AQP-1 is differentially sorted and released via the exos

167 The presence of AQP-0 in retinal bipolar cells was also demonstrated, wh

168 e discovery of its role in the regulation of AQP translocation has ramifications for diverse physiolo

169 To study the regulation of AQP-2, immature and adult rats were kept on ad libitum i

170 me inhibitor, MG132, suppresses secretion of AQP-1, implying that ubiquitination is a sorting signal

171 tonicity in vitro regulates the secretion of AQP-1, thus showing that extracellular osmotic condition

172 ane, whereas water loading caused a shift of AQP-2 channels back to intracellular vesicles in both ad

173 5) cm3/s, a value 30-fold lower than that of AQP 1, the red blood cell water channel.

174 ration and dDAVP stimulated translocation of AQP-2 from intracellular vesicles to the plasma membrane

175 based on a comparative mutagenic analysis of AQPs 1, 3, and 4, suggest that loop D interactions may p

176 colorectal carcinogenesis, the expression of AQPs 1 and 5 was induced in early-stage disease (early d

177 Expression of AQPs 1 and 5 was maintained even in metastatic lesions i

178 is showed that tissue-specific expression of AQPs 1, 2, 3, and 5 was not affected by AQP4 deletion.

179 erase chain reaction analysis, expression of AQPs 1, 3, and 5 was found in seven colon and colorectal

180 NAs to specifically reduce the expression of AQPs in epithelial cells and provides direct evidence of

181 unofluorescence suggested the involvement of AQPs 3, 4, and 5 in high airway water permeability.

182 Based on the involvement of AQPs in angiogenesis and cell migration, we tested wheth

183 /s, which is within 2 orders of magnitude of AQPs' rates, and reject all ions except protons.

184 on the function and regulation mechanism of AQPs.

185 An alternative is controlling the number of AQPs in the cell membrane.

186 different types of human cancer, the role of AQPs in carcinogenesis has not been clearly defined.

187 However, clarifying the role of AQPs in mediating water transport in biliary epithelia h

188 The role of AQPs in the hierarchy of the hydraulic signal pathway co

189 ut the effects of a hypotonic environment on AQP abundance.

190 The transcriptional effects of TAp73 on AQP-2 and B2R were independent of p53.

191 Importantly, only AQP and three novel DEGs associated with stress, mangane

192 Only AQPs exhibited significant osmotic water permeability (P

193 mercurial-insensitive water channel (MIWC or AQP-4) is a 30-32 kDA integral membrane protein expresse

194 reduced water conductance relative to other AQPs.

195 cm/s) of endosomes containing phosphorylated AQP-2 (0.7 +/- 0.3 mol of PO4/mol of protein) is not sig

196 Analysis of purified AQP-0 reconstituted in raft-containing bilayers showed t

197 el Pf, we compared the Pf values of purified AQP-2 endosomes after incubation with either PKA or alka

198 les gambiae contains at least seven putative AQP sequences.

199 on the regulation of the expression of renal AQP and NKCC2, studies were performed with hyperosmolali

200 is not significantly different from the same AQP-2 endosomes where 95 +/- 8% of the phosphate has bee

201 P) in cellular water permeability and screen AQP-specific drugs.

202 highly water and neutral substrate selective AQP family.

203 he case in the structures of water-selective AQPs AqpZ and AQP1, the asparagines of the 2 Asn-Pro-Ala

204 porins (AQP0-AQP12) cloned in mammals, seven AQPs have been identified in the liver and biliary tree.

205 s demonstrate that the expression of several AQPs is found in tumor cells and is associated with an e

206 de evidence against functionally significant AQP expression in mitochondria, which is consistent with

207 ted, in part because of the lack of specific AQP inhibitors.

208 We identified an efficient water-specific AQP (ClAQP1), two aquaglyceroporins (ClGlp1 and ClGlp2)

209 al when co-expressed with the water-specific AQP.

210 Quantification of baseline steady-state AQP-2 excretion was done by collecting urine on the day

211 Furthermore TAp73 stimulated AQP-2 promoter-driven reporter expression.

212 In the second study, AQP-2 expression was evaluated in different regions of k

213 In summary, AQP-2 expression and trafficking in the immature kidney

214 annel conductance that is 30-fold lower than AQP 1.

215 We conclude that AQP-mediated water transport in macrophages constitutes

216 Results demonstrate that AQP-1 is up-regulated in the small, angiogenic, neovascu

217 f this study was to test the hypothesis that AQP-1 is involved in amoeboid motility and angiogenic in

218 serum-responsive gene, we hypothesized that AQP expression may be involved in the development of hum

219 Taken together these results indicate that AQP-0 and connexins can be segregated in the membrane by

220 Studies using [gamma-32P]ATP reveal that AQP-2 endosomes contain endogenous PKA and phosphatase a

221 where freeze-fracture experiments show that AQP-0 oligomerizes (3).

222 Here, we show that AQP-1 is partially lost during in vitro maturation of mo

223 ults, confocal microscopy images showed that AQP-0 was sequestered into raft microdomains in the 1:10

224 These results lead us to suggest that AQP-1 sorting into exosomes may be the mechanism by whic

225 Our data demonstrate that AQPs have critical roles in excretion, water homeostasis

226 It was hypothesized that AQPs contribute to cell elongation processes by allowing

227 sed to water-related stress, suggesting that AQPs may offer novel control avenues.

228 Accumulating evidence suggests that AQPs are likely involved in canalicular and ductal bile

229 The AQP-expressing cells showed at least 10x faster osmotic

230 mosome locus 12q13, the same location as the AQP.2 and MIP genes.

231 pH did not differ significantly in the AQP knockout mice.

232 AQP3 is the AQP that is expressed in the skin where it facilitates c

233 hallmarks of the water-selective arm of the AQP family of proteins.

234 e been observed for different members of the AQP family, the signature homotetrameric quaternary stru

235 Unlike other members of the AQP family, the unique distribution in intracellular mem

236 oocytes with very low concentrations of the AQP inhibitors HgCl(2) and AgNO(3).

237 of Xenopus oocytes expressing members of the AQP or Rh family.

238 ents the hypothesis that measurements of the AQP-2 excretion rate might be used as a marker of collec

239 shown to depend on water-wires spanning the AQP pore that reverse their orientation, combined with c

240 anism of selective water passage through the AQP pore are established, but there remains a gap in our

241 controlling the passage of water through the AQP pore, but this only has been observed as a phenomeno

242 framework for tetrameric assembly within the AQP family.

243 We hypothesized that the AQPs of the vascular bundle sheath (BS) cells regulate K

244 this model, we define the importance of this AQP in water transport across biliary epithelia.

245 ed to acquire a better understanding of this AQP subfamily.

246 Some TIP-AQP genes, such as TIP2;2 and TIP1;1, are up-regulated u

247 s called tonoplast-intrinsic aquaporins (TIP-AQPs).

248 We propose that TIP-AQPs affect the induction of leaf abscisic acid, which l

249 ificant defect, yet to be defined, distal to AQP-2.

250 water channels with 2.6 A pores, similar to AQP channels, that encapsulate oriented dipolar water-wi

251 rin 1 (AgAQP1) protein that is homologous to AQPs known in humans, Drosophila, and sap-sucking insect

252 sis thaliana), the highly abundant tonoplast AQP isoforms AtTIP1;1, AtTIP1;2, and AtTIP2;1 facilitate

253 r the isotype control, failed to translocate AQP to the plasma membrane.

254 Urinary AQP-2 excretion correlated best with solute-free water c

255 Compared to placebo, urinary AQP-2 excretion decreased significantly and in all group

256 Thereafter, urinary AQP-2 excretion was calculated as a ratio of the urine f

257 Therefore, urinary AQP-2 excretion can be quantified by using Western analy

258 (2+) and Ni (2+) on the water transport via AQPs.

259 odium (SCNN1-B, ATP1-A1, ATP1-B1) and water (AQP-4) movement in the fetal lung.

260 nt soluble membrane (DSM) fractions, whereas AQP-0 was found in both detergent resistant membrane and

261 enesis and cell migration, we tested whether AQP expression in tumor cells might enhance their migrat