ライフサイエンスコーパス: ATG12

1 helix on a surface area that is exclusive to ATG12.

2 cape senescence express a high level of ATG5/ATG12.

3 Atg12, but NLRX1 did not associate with Atg5-Atg12.

4 aliana with a focus on the two loci encoding ATG12.

5 autophagy pathway genes LC3, Gabarapl1, and Atg12.

6 bind to promoter sequences of Gabarapl1 and Atg12.

7 We also found that the number of Atg12/16L1-positive puncta and recruitment of the Atg12-

8 ncta, and interacted with Atg5 and also with Atg12-5 conjugate.

9 Recruitment of the autophagy-associated Atg12-5-16L1 complex to Wipi2-positive phagophores is cr

10 some, by facilitating the recruitment of the Atg12-5-16L1 complex to Wipi2-positive phagophores.

11 /16L1-positive puncta and recruitment of the Atg12-5-16L1 complex to Wipi2-positive puncta are reduce

12 rane surrounding Salmonella and recruits the Atg12-5-16L1 complex, initiating LC3 conjugation, autoph

13 induced autophagy through recruitment of the Atg12-5-16L1 complex.

14 olic components requires the LC3-conjugating Atg12-5-16L1 complex.

15 The molecular mechanisms of Atg12-5-16L1 recruitment and significance of PtdIns(3)P

16 Of note, Optn was recruited to Atg12-5-16L1-positive puncta, and interacted with Atg5 a

17 tophagosome formation and recruitment to the Atg12-5-16L1-positive puncta.

18 knockdown of the autophagy-related gene 12 (Atg12) abolished the activation of autophagy and the inc

19 reduces acetylation of Atg5, Atg7, Atg8, and Atg12, although overexpressed p300 increases the acetyla

20 ment by ATG12 in higher eukaryotes and place ATG12 among the members of signaling ublps that bind lin

21 ATG12, an ubiquitin-like modifier required for macroauto

22 TUFM interacted with Atg5-Atg12 and Atg16L1 and has similar functions as NLRX1 by

23 tophagy by competing with ATG16 to bind ATG5/ATG12 and preventing ATG16/ATG5/ATG12 formation.

24 including MAFBx, MuRF1, p62, LC3B, Beclin1, Atg12, and Fn14.

25 agy activity and transcript levels of vps34, atg12, and gabarapl1 genes were reduced by insulin.

26 Levels of vps34, atg12, and gabarapl1 transcripts were elevated in the li

27 impaired LC3B-II conversion; reduced ATG14L, ATG12, and WIPI-1 puncta formation; and significantly de

28 ophagy-related (ATG)12 is a component of the ATG12 approximately ATG5-ATG16L1 E3 complex that promote

29 esidues whose side chains make contacts with ATG12 are important for E3 interaction as well as LC3 li

30 y proteins (i.e., Beclin-1, Atg4B, Atg5, and Atg12) are proviral factors required for translation of

31 eins including Vps34, autophagy gene (Atg)5, Atg12, Atg13, biochemical, and microscopy studies, we de

32 We found that depletion of Beclin-1, Atg12, Atg14, Atg16, or LC3 with specific small interfer

33 is co-localizes with autophagy factors ATG5, ATG12, ATG16L1, p62, NDP52, BECN1 and LC3, stimulation o

34 Thus, the Atg5-Atg12/Atg16L1 complex performs a pivotal, nondegradative

35 We show that the Atg5-Atg12/Atg16L1 protein complex, whose prior known functio

36 IFNgamma, via Atg5-Atg12/Atg16L1, inhibited formation of the membranous cyt

37 Rather, the lack of ATG12-ATG3 complex formation produces an expansion in mi

38 ATG12-ATG3 complex formation requires ATG7 as the E1 enz

39 Overall, these results unveil a role for ATG12-ATG3 in mitochondrial homeostasis and implicate th

40 ether, we conclude that the formation of the ATG12-ATG5 adduct is essential for ATG8-mediated autopha

41 le but is not essential for synthesizing the ATG12-ATG5 and ATG8-phosphatidylethanolamine adducts tha

42 Synthesis of the ATG12-ATG5 and ATG8-phosphatidylethanolamine adducts, wh

43 Moreover, t-TUCB restored hepatic levels of Atg12-Atg5 and LC3-II conjugates and reduced p62 express

44 show here that the subpathway that forms the ATG12-ATG5 conjugate also has an essential role in plant

45 Here we report that TECPR1 binds to the Atg12-Atg5 conjugate and phosphatidylinositol 3-phosphat

46 essential for ATG12 conjugation and that the ATG12-ATG5 conjugate is necessary to form autophagic ves

47 gle atg12a and atg12b mutants on forming the ATG12-ATG5 conjugate reveal that the ATG12b locus is mor

48 6 form mutually exclusive complexes with the Atg12-Atg5 conjugate, and TECPR1 binds PtdIns(3)P upon a

49 1 binds PtdIns(3)P upon association with the Atg12-Atg5 conjugate.

50 also show that ATG8 lipidation requires the ATG12-ATG5 conjugate.

51 plants missing ATG5 or ATG7, cannot form the ATG12-ATG5 conjugate.

52 We have shown previously that the Atg12-Atg5 conjugation system, required for autophagosom

53 hat the concerted interactions among TECPR1, Atg12-Atg5, and PtdIns(3)P provide the fusion specificit

54 zation of a second ubiquitin-like conjugate, Atg12-Atg5, suggests that Atg21 may be involved in the r

55 ATG5 is a key component of an E3-like ATG12-ATG5-ATG16 protein complex that catalyzes conjugat

56 lethanolamine, including Atg7, Atg3, and the Atg12-Atg5-Atg16L1 complex play crucial roles in the con

57 The interaction of the Atg12~Atg5-Atg16 complex and Atg8 with Atg19 is mutually

58 The interaction of Atg19 with the Atg12~Atg5-Atg16 complex is mediated by its Atg8-interac

59 o receptors interact with the E3-like enzyme Atg12~Atg5-Atg16, which stimulates Atg8 conjugation.

60 mma against MNV in macrophages required Atg5-Atg12, Atg7, and Atg16L1, but not induction of autophagy

61 hagy-related protein 7 (Atg7), Beclin-1, and Atg12, autophagy regulatory proteins, was analyzed by we

62 proapoptotic role for the autophagic protein Atg12, based on a BH3-like domain, which enables binding

63 icated by altered expression and activity of ATG12, beclin, p62, and LC3A II, hallmarks of autophagy,

64 enic mice show lower levels of Dock180, LC3, Atg12, Becn1, Rab5 and Rubicon in Sertoli cells.

65 Here we report the identification of the ATG12 binding sequence in the flexible region of human A

66 tions of the autophagy-related proteins Atg5-Atg12, but NLRX1 did not associate with Atg5-Atg12.

67 ant increase of pro-autophagic proteins ATG5-ATG12 COMPLEX and Beclin-1.

68 its casp8 through interaction with FADD:Atg5-Atg12 complexes.

69 Protein levels of Beclin-1, Atg5-Atg12 conjugate, and LC3-II were also significantly redu

70 results indicate that ATG10 is essential for ATG12 conjugation and that the ATG12-ATG5 conjugate is n

71 7 or Atg5 or blocking LC3 lipidation or ATG5-ATG12 conjugation decreases ERK phosphorylation.

72 To characterize the ATG12 conjugation pathway specifically, we characterized

73 mitochondrial homeostasis and implicate the ATG12 conjugation system in cellular functions distinct

74 Surprisingly, disrupting ATG12 conjugation to ATG3 does not affect starvation-ind

75 DFCP1 to autophagic precursors, reduced ATG5-ATG12 conjugation, and compromised autophagosome formati

76 Here, we identify ATG3 as a substrate for ATG12 conjugation.

77 son reveals that the ATG3 binding surface on ATG12 contains a hydrophobic pocket corresponding to the

78 Unlike ATG8, ATG12 does not associate with autophagic bodies, implyin

79 to bind ATG5/ATG12 and preventing ATG16/ATG5/ATG12 formation.

80 tablish the mechanism of ATG3 recruitment by ATG12 in higher eukaryotes and place ATG12 among the mem

81 A role of ATG12 in the E3 complex is to recruit the E2 enzyme ATG3

82 on of key autophagy effectors (such as ATG5, ATG12, LC3B and LAMP1) and AMPK-dependent activation of

83 lly and characterized it genetically through atg12 mutants that block ATG8 modification.

84 the E2, resulting in the covalent linkage of ATG12 onto a single lysine on ATG3.

85 Phenotypic analyses showed that atg12 plants are phenotypically normal and fertile when

86 atg12 plants have compromised autophagic transport as de

87 revealed that remobilization is impaired in atg12 plants, which significantly decreases seed yield a

88 or by depletion of the autophagy-related-12 (Atg12) protein homolog.

89 hat couple the AUTOPHAGY-RELATED8 (ATG8) and ATG12 proteins to their respective targets, phosphatidyl

90 autophagy events represented by ATG16L1 and ATG12 puncta formation.

91 Atg7 is required for the conjugation of Atg12 to Atg5, and Atg8 to phosphatidylethanolamine (PE)

92 autophagy flux and defects in conjugation of ATG12 to ATG5.

93 the two ubiquitin-fold polypeptides ATG8 and ATG12 to phosphatidylethanolamine and the ATG5 protein,

94 of two ubiquitin-like protein tags, ATG8 and ATG12, to phosphatidylethanolamine and the ATG5 protein,

95 jugation machinery in the SUMO, NEDD8, ATG8, ATG12, URM1, UFM1, FAT10, and ISG15 pathways while refer

96 quired to initiate ligation of both ATG8 and ATG12, we previously showed that the ATG8/12 conjugation

97 -fold proteins Autophagy-related (ATG)-8 and ATG12, which become attached to the lipid phosphatidylet

98 A key component in ATG8 function is ATG12, which promotes lipidation upon its attachment to