ライフサイエンスコーパス: ATP-gamma-S

1 ATP gamma S binding stabilized ClpA and ClpX such that t

2 ATP gamma S plus Ca induced a weak-binding state with mo

3 ATP-gamma-S and 2-methylthio ATP (2-Me-S-ATP) were signi

4 ATP-gamma-S is occluded into the Pgp NBDs at 34 degrees

5 ATP-gamma-S, a poorly hydrolyzable ATP analog, did not s

6 In particular, Mg(2+)GTP gamma S and Mg(2+)ATP gamma S inhibited cyclase activity through a mixed,

7 ed by adding the non-hydrolyzable ATP analog ATP-gamma-S.

8 r presence of the poorly hydrolyzable analog ATP-gamma-S unexpectedly revealed that a large number of

9 nstrate that the nonhydrolyzable ATP analog, ATP gamma S, supports the formation of an isolable leadi

10 Using the nonhydrolyzable ATP analogue ATP-gamma-S results in formation of a nonproductive gp45

11 n by use of the nonhydrolyzable ATP analogue ATP-gamma-S.

12 nce of ATP or its slowly hydrolyzed analogue ATP-gamma-S, under conditions where a significant fracti

13 d Mn on wild-type and D314E Csk with ATP and ATP gamma S [adenosine 5'-O-(3-thiotriphosphate)] as sub

14 wed either by providing a mixture of ATP and ATP gamma S, a nonphysiological and slowly hydrolyzed AT

15 y active ATP analogs (trinitrophenyl-ATP and ATP gamma S-acetamidoproxyl), to form stable binary comp

16 cyl transfer was stimulated by ADP, ATP, and ATP gamma S, but not by other nucleotides.

17 Two ATP analogs, FSBA and ATP gamma S, used in this study, were both found to bind

18 The nonhydrolyzable analog AMP-PNP and ATP-gamma S also stimulate ADP release from the second h

19 fected the k(cat) values of both the ATP and ATP-gamma-S reactions with a slope of about 0.8.

20 Rad51 binds ATP and ATP-gamma-S with a 1:1 stoichiometry and Kds of 21 and 1

21 for 21 days with osteogenic media, ATP, and ATP-gamma-S (a stable agonist of the P2Y receptor) revea

22 helicase protein in the presence of DNA and ATP-gamma-S (1 primase:1 helicase monomer; K(d) approxim

23 -nucleotides including GTP, UTP, AMP-PCP and ATP-gamma-S, but not by PP(i) or AMP, suggesting that ch

24 nder different conditions, using either ATP, ATP gamma S, AMP-PNP or ADP as nucleotide cofactors, we

25 hich differed when a nucleotide (either ATP, ATP gamma S, or ADP) was bound.

26 ith the thiophosphate donor analogue of ATP, ATP gamma S, or protein phosphatase 1 and 2A inhibitors

27 weakest bound state of the motor, while ATP, ATP gamma S, AMPPNP, and apyrase all induce a shift towa

28 BzATP, alpha, beta-Me-ATP, ATP-gamma-S and 2-Me-S-ATP induced single-channel curren

29 s nonproductive complex results in rapid ATP/ATP-gamma-S exchange to yield a productive gp45 x gp44/6

30 tions in CBF and [Ca(2+)](i) were induced by ATP- gamma -S.

31 e forskolin effect was strongly augmented by ATP-gamma-S.

32 Inhibition of ATP hydrolysis by ATP-gamma-S did not have a measurable effect upon holoen

33 nary complex with ClpX that is stabilized by ATP[gamma-S].

34 Although unable to promote binding to DNA, ATP-gamma-S also prevents inactivation of Rad51 at 37 de

35 d an apparent dissociation constant (Kd) for ATP gamma S of 150 microM.

36 The Kcat thio effects [Kcat(ATP)/Kcat(ATP gamma S)] were inversely correlated with metal thiop

37 6) M, dTDP; 3 x 10(-5) M, ATP; 2 x 10(-6) M, ATP gamma S), while binding to the remaining sites is un

38 ddition, dialysis of neurons with 500 microM ATP-gamma-S did not alter the acute effect of BDM.

39 ATP, but not nonhydrolyzable ATP-gamma S, regulates the SODD binding by Hsp70 or TNFR

40 independent increase in CBF, neither ATP nor ATP- gamma -S induced an increase in CBF when the Ca(2+)

41 s observed with NTRC S160F in the absence of ATP gamma S (Kd = 0.5 microM).

42 The addition of ATP gamma S induced a significant association of NTRC S1

43 treatment of cytosol, as well as addition of ATP gamma S to the chase incubations, led to a stabiliza

44 The application of ATP gamma S(in) occluded the effect of 17 beta-oestradio

45 in with a K(d) = 4 microM in the presence of ATP gamma S at 50 mM KCl, which is 10-20-fold tighter th

46 In the presence of ATP gamma S the equilibrium was shifted towards the dime

47 In sideviews of ATP gamma S-stabilized ClpXP complexes, a narrow axial c

48 While the affinity of ATP-gamma-S is the same as the affinity of ATP, the affi

49 DNA has a smaller effect on the affinity of ATP-gamma-S.

50 P2Y2 mRNA by 75% and blocked the effects of ATP-gamma-S by 65%.

51 s of the dependence of this insensitivity on ATP gamma S concentration suggested an apparent dissocia

52 litated by Mg(2+) ions but not by AMP-PNP or ATP gamma S.

53 e domain in its apo-state or bound to ADP or ATP-gamma-S show conformations reminiscent of the activa

54 aments formed in the presence of ATP, ADP or ATP-gamma-S showed that efficient Rad51 turnover from ds

55 YO-1 dye molecules in the presence of ATP or ATP-gamma-S.

56 n the Cdc6p Walker A and Walker B motifs, or ATP-gamma-S inhibited these activities of Cdc6p.

57 Apical UTP or ATP-gamma-S stimulation of monolayers mounted in Ussing

58 elded several fragments that differed in the ATP gamma S and ADP-bound forms, and thus revealed confo

59 The ATP-gamma-S bound and unbound crystal structures of the

60 5-HT and adenosine-5'-O-3-thiotriphosphate (ATP-gamma-S) to acutely isolated ciliated ependymal cell

61 Adenosine 5'-O-(3-thiotriphosphate) (ATP gamma S) relaxed the muscle fiber, completely dissoc

62 P), and adenosine 5'-O-(3-thiotriphosphate) (ATP gamma S).

63 P), and adenosine 5'-O-(3-thiotriphosphate) (ATP-gamma-S) bind with similar affinities to the high-af

64 of ATP, adenosine 5'-O-[3-thiotriphosphate] (ATP gamma S) and alpha,beta methylene adenosine triphosp

65 ce of adenosine 5'-[gamma-thio]triphosphate (ATP[gamma-S]), in a temperature regulated high pressure

66 Upon ATP gamma S-induced dissociation, the leading strand pol

67 d the agonist rank potency order ATP = UTP = ATP gamma S > ADP > AMP > > adenosine; adenine, AMP-CPP,

68 kinase reaction was lowered by 1.8-fold when ATP-gamma-S was used.

69 ffective in releasing Mge1 from Ssc1 whereas ATP gamma S and AMPPNP could not disrupt the Ssc1/Mge1 c

70 atment with MG132 or replacement of ATP with ATP-gamma-S, a nonhydrolyzable analogue of ATP, suggesti

71 Furthermore, intracellular perfusion with ATP-gamma-S augments channel activation.