1 uent affinity-enrichment analyses identified AUF-1 and YB-1, 2 cytoplasmic proteins with well-establi

2 by quantum chemical calculations that (a) Zn(AuF(6))(3) is not a thermochemically feasible compound,

3 raction of Zn with the ligands F, BO(2), and AuF(6), whose electron affinities are progressively high

4                       Deletion of the entire auf gene cluster had no effect on the ability of CFT073

5 , and 48 h of infection, indicating that the auf gene cluster is expressed in the murine urinary trac

6                          To determine if the auf gene cluster is expressed in vivo, RT-PCR was perfor

7 nder these conditions, the expression of the auf gene cluster is low.

8 luster, a recombinant plasmid containing the auf gene cluster under the T7 promoter was introduced in

9 . coli and related species revealed that the auf gene cluster was significantly associated with uropa

10     To identify the structure encoded by the auf gene cluster, a recombinant plasmid containing the a

11      For in vitro expression analysis of the auf gene cluster, RNA was isolated from CFT073 bacteria

12                                   Therefore, Auf is a uropathogenic E. coli-associated structure that