ライフサイエンスコーパス: Aquifex

1 d on the stem structures associated with the Aquifex 16S and 23S rRNA precursors are cleaved at sites

2 ss I BPL from the hyperthermophilic bacteria Aquifex aeolicus (AaBPL) in its ligand-free form and in

3 rom the hyperthermophilic, ancient bacterium Aquifex aeolicus (Aacpn10) has a 25-residue C-terminal e

4 deep-branching, hyperthermophilic bacterium Aquifex aeolicus (Aacpn10) shows high homology with meso

5 atured cpn10 from Homo sapiens (hmcpn10) and Aquifex aeolicus (Aacpn10) were monitored by far-UV circ

6 meric co-chaperonin proteins 10 (cpn10) from Aquifex aeolicus (Aacpn10-del25) and human mitochondria

7 al structure of the leucine transporter from Aquifex aeolicus (aaLeuT) has provided significant insig

8 end, we investigated a thermostable LS from Aquifex aeolicus (AaLS) and found that it also forms cag

9 empted to convert the capsid-forming LS from Aquifex aeolicus (AaLS) into pentamers through a small n

10 l, 60-subunit capsid, lumazine synthase from Aquifex aeolicus (AaLS), to act as a container for nucle

11 chaeum equitans, Pyrobaculum aerophilum, and Aquifex aeolicus (all hyperthermophiles).

12 ral gene fliA was exchanged with homologs of Aquifex aeolicus (an extreme thermophile) and Chlamydia

13 The ribosomal protein S8 from Aquifex aeolicus (AS8) is unique in that there is a 41-r

14 al structure of the leucine transporter from Aquifex aeolicus (LeuT).

15 e present the crystal structure of MraY from Aquifex aeolicus (MraYAA) at 3.3 A resolution, which all

16 e present the crystal structure of MraY from Aquifex aeolicus (MraYAA) in complex with its naturally

17 Aquifex aeolicus 3-deoxy-D-manno-octulosonate 8-phosphat

18 Aquifex aeolicus 3-deoxy-d-manno-octulosonate 8-phosphat

19 The [2Fe-2S] ferredoxin (Fd4) from Aquifex aeolicus adopts a thioredoxin-like polypeptide f

20 We report the crystal structure of Aquifex aeolicus Ago (Aa-Ago) together with binding and

21 CAK dynamics with those of hyperthermophilic Aquifex aeolicus AK (AAAK), our results provide strong e

22 crystal structures of an active fragment of Aquifex aeolicus alanyl-tRNA synthetase complexed, separ

23 crystal structure of a catalytic fragment of Aquifex aeolicus AlaRS and additional data suggest how t

24 of the 453 amino acid catalytic fragment of Aquifex aeolicus AlaRS.

25 teases from two different bacterial species, Aquifex aeolicus and B. subtilis.

26 ed the MpgII genes from T. maritima and from Aquifex aeolicus and found that both genes could restore

27 similar studies done with SPS orthologs from Aquifex aeolicus and humans, we propose a catalytic mech

28 The amt genes of the hyperthermophiles Aquifex aeolicus and Methanococcus jannaschii complement

29 le for two different PGT domains, PBP1A from Aquifex aeolicus and PBP1A from Escherichia coli.

30 ture of NusB from the thermophilic bacterium Aquifex aeolicus and studied the interaction of NusB and

31 rt the kinetic characterization of LpxK from Aquifex aeolicus and the crystal structures of LpxK in c

32 terized aldolases of Helicobacter pylori and Aquifex aeolicus and to the group that comprises the Cal

33 Escherichia coli and the metallo KDO8PS from Aquifex aeolicus are the best characterized members of e

34 d of CTP and ATP; we transformed the related Aquifex aeolicus CC- and A-adding enzymes into UU- and G

35 KDO8PS) from the hyperthermophilic bacterium Aquifex aeolicus differs from its Escherichia coli count

36 was used to probe conformational changes of Aquifex aeolicus dihydroorotase (DHO), which catalyzes t

37 The X-ray structures of Aquifex aeolicus dihydroorotase in two space groups, C22

38 solution that the ATP-dependent assembly of Aquifex aeolicus DnaA into a spiral oligomer creates a c

39 d the structure of the conserved core of the Aquifex aeolicus DnaA protein to 2.7 A resolution.

40 Based on the structural similarity of Aquifex aeolicus DnaA to other AAA+ proteins that are ol

41 A 3.3 A crystal structure of Aquifex aeolicus DnaB, complexed with nucleotide, reveal

42 The genome of the bacterium Aquifex aeolicus encodes a polypeptide which is related

43 The phoU gene of Aquifex aeolicus encodes a protein called PHOU_AQUAE wit

44 The deeply rooted organism Aquifex aeolicus encodes one type IIA topoisomerase conf

45 ly homologous and structurally characterized Aquifex aeolicus ferredoxin 4 (AaeFd4) using EPR, UV-vis

46 The hyperthermophilic bacterium Aquifex aeolicus has a MutL protein (Aae MutL) that poss

47 conserved hypothetical protein, Aq1575, from Aquifex aeolicus has been determined by using x-ray crys

48 ecent NMR and X-ray studies of the LpxC from Aquifex aeolicus have provided the first structural info

49 tulosonate 8-phosphate (KDO8P) synthase from Aquifex aeolicus in complex with phosphoenolpyruvate (PE

50 The placement of the extreme thermophile Aquifex aeolicus in the bacterial phylogenetic tree has

51 of the alpha2beta2 GlyRS from the bacterium Aquifex aeolicus is able to perform the first step of th

52 have investigated the mechanism of action of Aquifex aeolicus IspH [E-4-hydroxy-3-methyl-but-2-enyl d

53 We report the inhibition of the Aquifex aeolicus IspH enzyme (LytB, (E)-4-hydroxy-3-meth

54 bstrate permeation pathway in the homologous Aquifex aeolicus leucine transporter.

55 us ( Tth ) ligase, Thermus sp. AK16D ligase, Aquifex aeolicus ligase and the K294R mutant of the Tth

56 h amino acid was altered in both E. coli and Aquifex aeolicus LpxC and the catalytic activities of th

57 k(cat)/Km catalyzed by Escherichia coli and Aquifex aeolicus LpxC displayed a bell-shaped curve (EcL

58 h the wild type (WT) and the H265A mutant of Aquifex aeolicus LpxC, each in the absence of substrate

59 ctures of apo- and ADP/Mg(2+)-bound forms of Aquifex aeolicus LpxK to a resolution of 1.9 A and 2.2 A

60 ared and studied, His42, His124, and Glu126 (Aquifex aeolicus numbering), with particular attention p

61 Relative to EcNusG, Aquifex aeolicus NusG (AaNusG) and several other bacteri

62 doglycan glycosyltransferase (PGT) domain of Aquifex aeolicus PBP1A.

63 4Fe-3S] cluster in hydrogenase (Hase) I from Aquifex aeolicus performs two redox transitions within a

64 When introduced into purified Aquifex aeolicus PilT, substitutions in the AIRNLIRE mot

65 D structure of the central domain from NtrC1 Aquifex aeolicus protein into our 3D model; we propose t

66 and genetic approaches that CCA addition in Aquifex aeolicus requires collaboration between two rela

67 gnetic resonance (NMR) analysis of SmpB from Aquifex aeolicus revealed an antiparallel beta-barrel st

68 [2Fe-2S] cluster containing ferredoxin from Aquifex aeolicus reveals a thioredoxin-like fold that is

69 crystal structure of the nuclease domain of Aquifex aeolicus RNase III, the E41, D114, and E117 side

70 Here, we present two crystal structures of Aquifex aeolicus SD, including a ternary complex with bo

71 previously determined crystal structures of Aquifex aeolicus SelA complexed with tRNA(Sec) revealed

72 tal structures of GAF regulatory domains for Aquifex aeolicus sigma(54) activators NifA-like homolog

73 We identified a minimal construct of the Aquifex aeolicus sigma(54) AID that consists of two pred

74 We report here the structure of an Aquifex aeolicus sigma(54) domain (residues 69-198), whi

75 e same affinity for the Escherichia coli and Aquifex aeolicus SmpB proteins as the intact E. coli tmR

76 Trbp111 is a 111 amino acid Aquifex aeolicus structure-specific tRNA-binding protein

77 protein from the hyperthermophilic bacterium Aquifex aeolicus suggested that this protein functions s

78 mily members, we determined the structure of Aquifex aeolicus ThiL (AaThiL) with nonhydrolyzable AMP-

79 n of the bacterial helicase loader DnaC from Aquifex aeolicus to 2.7 A resolution.

80 ligase from the hyperthermophilic bacterium Aquifex aeolicus was cloned, expressed in Escherichia co

81 homologue from the thermophilic eubacterium Aquifex aeolicus was cloned, overexpressed, and purified

82 Aquifex aeolicus was one of the earliest diverging, and

83 namide ribonucleotide synthetase (GARS) from Aquifex aeolicus were expressed in Escherichia coli, and

84 fashion, whereas the Class II enzymes (e.g. Aquifex aeolicus) require metal ions for catalysis.

85 or associate into multifunctional complexes (Aquifex aeolicus).

86 tion structure of a leucine transporter from Aquifex aeolicus, a bacterial member of the SLC6 transpo

87 out the active site environment of LpxC from Aquifex aeolicus, a heat-stable orthologue that displays

88 The enzyme from Aquifex aeolicus, a hyperthermophilic organism of ancien

89 -bound forms of the DBD of NtrC4 (4DBD) from Aquifex aeolicus, a member of the NtrC family of sigma(5

90 of the free and CMP-bound forms of WaaA from Aquifex aeolicus, an ancient Gram-negative hyperthermoph

91 Aquifex aeolicus, an extreme hyperthermophile, has neith

92 of intact NtrC4 (a sigma(54) activator from Aquifex aeolicus, an extreme thermophile), as well as it

93 Aquifex aeolicus, an organism that flourishes at 95 degr

94 n from Escherichia coli, a KtrB protein from Aquifex aeolicus, and a Trk1,2 protein from Schizosaccha

95 studies of NtrC4, a sigma-54 activator from Aquifex aeolicus, and compare it with NtrC1 (a paralog)

96 e LpxC deacetylase from the hyperthermophile Aquifex aeolicus, and it has excellent antibiotic activi

97 L27, was cloned from the extreme thermophile Aquifex aeolicus, and the protein was overexpressed and

98 erichia coli, Agrobacterium tumefaciens, and Aquifex aeolicus, as well as the ADAT2-ADAT3 proteins fr

99 charomyces cerevisiae, and from the bacteria Aquifex aeolicus, Borrelia burgdorferi, Clostridium stic

100 A hemoglobin was identified in Aquifex aeolicus, cloned, recombinantly expressed, purif

101 GatCAB from the hyperthermophilic bacterium Aquifex aeolicus, complexed with glutamine, asparagine,

102 The thermophilic chemolithotroph, Aquifex aeolicus, expresses a gene product that exhibits

103 nanomolar concentrations, including that of Aquifex aeolicus, for which structural information is av

104 e E/G homologs from phylogenetically distant Aquifex aeolicus, Haemophilus influenzae Rd, and Synecho

105 mains from heterologous organisms, including Aquifex aeolicus, localized to septal rings when produce

106 Here, we demonstrate that KDO8PS from Aquifex aeolicus, representing only the second member of

107 enzymes from human, Myxococcus xanthus, and Aquifex aeolicus, respectively.

108 mologue from the hyperthermophilic bacterium Aquifex aeolicus, that shares 35.2% identity with human

109 Given the ancient lineage of Aquifex aeolicus, this observation provides the first in

110 and ATP; however, we recently found that in Aquifex aeolicus, which lies near the deepest root of th

111 we report the crystal structure of LpxC from Aquifex aeolicus, which reveals a new alpha+beta fold re

112 , including one from the extreme thermophile Aquifex aeolicus, which suggests that RusA may be of anc

113 avorably with the -tolerant hydrogenase from Aquifex aeolicus, which we use here as a benchmark.

114 re, we present new structures of FtsZ from47 Aquifex aeolicus,47 Bacillus subtilis, Methanococcus jan

115 e NtrC1 protein from the extreme thermophile Aquifex aeolicus.

116 tG6PDH) from the hyperthermophilic bacterium Aquifex aeolicus.

117 Delta 67) from the extreme hyperthermophile Aquifex aeolicus.

118 he hyperthermophiles Thermotoga maritima and Aquifex aeolicus.

119 cation apparatus of the extreme thermophile, Aquifex aeolicus.

120 hromosome of the hyperthermophilic bacterium Aquifex aeolicus.

121 roteins from the hyperthermophilic bacterium Aquifex aeolicus.

122 and purified LpxC from the hyperthermophile Aquifex aeolicus.

123 e traffic in the hyperthermophilic bacterium Aquifex aeolicus.

124 mbranes of the hyperthermophilic eubacterium Aquifex aeolicus.

125 of TatC from the hyperthermophilic bacterium Aquifex aeolicus.

126 enzyme from the hyperthermophilic bacterium, Aquifex aeolicus.

127 e of the leucine transporter (LeuT(Aa)) from Aquifex aeolicus.

128 ure of the C-terminal domain of sigma54 from Aquifex aeolicus.

129 y RNase P in the hyperthermophilic bacterium Aquifex aeolicus: Without an RNA subunit and the smalles

130 Despite low expression levels, His-tagged Aquifex Amt could be purified by heating and nickel chel

131 ic analysis of ribosomal proteins identifies Aquifex as grouping with Thermotoga another bacterial hy

132 Comparison of the Aquifex ATP sulfurylase active site with those from sulf

133 2.3 A resolution X-ray crystal structure of Aquifex ATP sulfurylase-APS kinase bifunctional enzyme i

134 s of Group I, which includes Proteobacteria, Aquifex, Chlamydia, Spirochaetes, Cytophaga-Chlorobium,

135 al HJRs in low-GC Gram-positive bacteria and AQUIFEX: Endonuclease VII of phage T4 is shown to serve

136 The thermal stability of the Aquifex enzyme can be explained by the 43% decreased cav

137 The domain arrangement in the Aquifex enzyme is reminiscent of the fungal ATP sulfuryl

138 Therefore, the Aquifex enzyme may represent an ancestral homolog of a p

139 th previous studies on Thermus ligases, this Aquifex ligase exhibits greater discrimination against a

140 ative polyA polymerases in Synechocystis and Aquifex, PRUNE of Drosophila, and an exopolyphosphatase

141 erall stereochemistry of the metal-dependent Aquifex pyrophilus KDO8P synthase (ApKDO8PS) reaction wa

142 ucture, but differing substantially from the Aquifex pyrophilus RacE structure.

143 of MurI from the hyperthermophilic bacterium Aquifex pyrophilus, we performed molecular dynamics (MD)

144 Homologs of Aquifex RNase P (HARP) were identified in many Archaea a

145 s, Bartonella, Nitrosomonas, Thermotoga, and Aquifex showed a strong preference for L-ornithine, wher

146 e played a role in several lineages, such as Aquifex, Thermotoga, and Fusobacterium.

147 deviation from this evolutionary pattern is Aquifex whose DdRp subunits cluster within Group I, wher