1 Asn-tRNA or Gln-tRNA formation in most prokaryotes requi
2 an amidotransferase of [14C]Asp-tRNA to [
14C]
Asn-tRNA.
3 we show that C. acetobutylicum forms Asn
and Asn-tRNA(Asn) by tRNA-dependent amidation.
4 the direct and indirect pathways of Asn
and Asn-tRNA(Asn) formation.
5 putatively involved in asparagine (Asn)
and Asn-tRNA(Asn) synthesis.
6 ny bacteria biosynthesize Gln-tRNA (Gln)
and Asn-tRNA (Asn) by an indirect, two-step pathway that rel
7 Many bacteria form Gln-tRNA(Gln)
and Asn-tRNA(Asn) by conversion of the misacylated Glu-tRNA(
8 required in vivo for both Gln-tRNA(Gln)
and Asn-tRNA(Asn) synthesis.
9 The amide aminoacyl-tRNAs, Gln-tRNA(Gln)
and Asn-tRNA(Asn), are formed in many bacteria by a pretrans
10 Moreover, both Gln-tRNA
and Asn-tRNA transamidation activities are present.
11 AB-encoded amidotransferase to generate
both Asn-tRNA and Gln-tRNA.
12 n an indirect path for the synthesis of
both Asn-tRNA(Asn) and Gln-tRNA(Gln).
13 urans and Bacillus subtilis encode AsnRS
for Asn-tRNA(Asn) formation and Asn synthetases to synthesiz
14 activity, GatDE for Gln-tRNA and GatCAB
for Asn-tRNA synthesis.
15 eria are predicted to use only one route
for Asn-tRNA(Asn) formation.
16 ia that contain asparagine synthetase A
form Asn-tRNA(Asn) by direct acylation catalyzed by asparagin
17 rmautotrophicus GatCAB as being able to
form Asn-tRNA(Asn) and Gln-tRNA(Gln), our data demonstrate th
18 ansferase and the presence of AsnRS,
forming Asn-tRNA by direct aminoacylation.
19 However,
Asn-tRNA synthesis in archaea is divergent: some archaea
20 at CAB) genes that modify Asp-tRNA(Asn)
into Asn-tRNA(Asn) and Glu-tRNA(Gln) into Gln-tRNA(Gln); (iv)
21 apacity of AdT to convert Asp-tRNA(Asn)
into Asn-tRNA(Asn) by approximately 35-fold.
22 ynthetase to AdT, where it is converted
into Asn-tRNA(Asn).
23 nverted Asp-tRNA(Asn) and Glu-tRNA(Gln)
into Asn-tRNA and Gln-tRNA, respectively.
24 on pathway also operates in the formation
of Asn-tRNA in these organisms.
25 esence of both direct and indirect routes
of Asn-tRNA and Gln-tRNA formation.
26 s require Asp-tRNA(Asn) for the synthesis
of Asn-tRNA(Asn).
27 form the cognate products, Gln-tRNA(Gln)
or Asn-tRNA(Asn).
28 own how archaea and some bacteria
synthesize Asn-tRNA and Gln-tRNA.
29 RNA formed by M. thermautotrophicus AspRS
to Asn-tRNA by M. thermautotrophicus Asp-tRNA(Asn) amidotra
30 e of apparently redundant complete routes
to Asn-tRNA formation, combined with the absence from the D
31 any prokaryotes synthesize asparaginyl-
tRNA (
Asn-tRNA(Asn)) in a similar manner using a non-discrimin
32 Synthesis of asparaginyl-
tRNA (
Asn-tRNA(Asn)) in bacteria can be formed either by direc
33 Asparaginyl-
tRNA (
Asn-tRNA) and glutaminyl-tRNA (Gln-tRNA) are essential c
34 Asparaginyl-
tRNA (
Asn-tRNA) is generated in nature via two alternate route
35 However, a smaller number of aa-
tRNAs (
Asn-tRNA, Gln-tRNA, Cys-tRNA and Sec-tRNA) are made by s
36 nerate one of the two amide aminoacyl-
tRNAs,
Asn-tRNA or Gln-tRNA, by transamidation of mischarged As