ライフサイエンスコーパス: Azotobacter

1 m the gram-negative bacteria Pseudomonas and Azotobacter.

2 Azotobacter chroococcum expresses iron-rich nitrogenases

3 ly one report on the isolation of FeVco from Azotobacter chroococcum.

4 The Azotobacter FeSII protein, also known as the Shethna pro

5 Ensifer, Rhodospirillaceae, Clostridium, and Azotobacter) operational taxonomic units (OTUs).

6 The nitrogenase component proteins in an Azotobacter strain bearing the double lysine mutation (i

7 We previously characterized two Azotobacter type III PKSs (ArsB and ArsC) with different

8 During the evolution of the 7Fe azotobacter-type ferredoxins from the 8Fe clostridial-ty

9 The properties of CO-inhibited Azotobacter vinelandii (Av) Mo-nitrogenase (N2ase) have

10 lysis of the two-component protein system of Azotobacter vinelandii (Av) nitrogenase is reported.

11 n this paper, we compare the interactions of Azotobacter vinelandii (Av) nitrogenase with two diazene

12 The nitrogenase VFe protein of Azotobacter vinelandii (Av1') has been shown to exist in

13 of this complex generated with proteins from Azotobacter vinelandii (designated the L127Delta-Av2-Av1

14 Here, a mutant strain of Azotobacter vinelandii (DJ1193) was used to facilitate t

15 of a new group II intron from the bacterium Azotobacter vinelandii (the AV intron).

16 The refined structure of reduced Azotobacter vinelandii 7Fe ferredoxin FdI at 100 K and 1

17 he ferricyanide-oxidized [4Fe-4S] cluster in Azotobacter vinelandii 7Fe ferredoxin, the spectroscopic

18 In this work, cluster transfer from Azotobacter vinelandii [Fe(2)S(2)](2+) cluster-bound Isc

19 e, it is shown that production of the intact Azotobacter vinelandii [Fe-S] cluster biosynthetic machi

20 in vitro analysis of two CooA homologs from Azotobacter vinelandii and Carboxydothermus hydrogenofor

21 is presented that nitrogenases isolated from Azotobacter vinelandii and Clostridium pasteurianum can

22 , crystal structures of the Fe-proteins from Azotobacter vinelandii and Clostridium pasteurianum have

23 s-NifEN protein from a DeltanifHDK strain of Azotobacter vinelandii and have found that the amounts o

24 and Pseudomonas putidabut not as strictly in Azotobacter vinelandii and not at all in P. aeruginosa.

25 lity we analyzed the genome of the bacterium Azotobacter vinelandii and show that genes that code for

26 Unlike glnD mutations in Azotobacter vinelandii and some other bacteria, glnD del

27 oxidoreductase was cloned and sequenced from Azotobacter vinelandii and termed the dsbA locus.

28 The NifS and NifU proteins from Azotobacter vinelandii are required for the full activat

29 We used Fe protein from Azotobacter vinelandii as the substrate to assess the ac

30 he structure of the precursor-bound NifEN of Azotobacter vinelandii at 2.6 angstrom resolution.

31 Azotobacter vinelandii bacterioferritin (AvBF) containin

32 nite-reduced (at pH 8.0) forms of the native Azotobacter vinelandii bacterioferritin to 2.7 and 2.0 A

33 The NifA protein from Azotobacter vinelandii belongs to a family of enhancer b

34 transcription of nitrogen fixation genes in Azotobacter vinelandii by modulating the activity of the

35 em for the controlled expression of genes in Azotobacter vinelandii by using genomic fusions to the s

36 itrogen fixation by the free-living organism Azotobacter vinelandii can occur through the activity of

37 The vanadium (V)-nitrogenase of Azotobacter vinelandii catalyses the in vitro conversion

38 eMo-co synthesis system is not required when Azotobacter vinelandii cell-free extract is prepared in

39 The vnf-encoded nitrogenase from Azotobacter vinelandii contains an iron-vanadium cofacto

40 The nitrogen-fixing organism Azotobacter vinelandii contains at least two systems tha

41 The NifL regulatory protein from Azotobacter vinelandii contains tandem PAS domains, the

42 The Azotobacter vinelandii cytochrome c5 gene (termed cycB)

43 xtracts from two different mutant strains of Azotobacter vinelandii defective in the biosynthesis of

44 e NifS protein was purified from a strain of Azotobacter vinelandii deleted for the nifS gene.

45 e structure of the catalytic domain from the Azotobacter vinelandii dihydrolipoamide acetyltransferas

46 rotein expressed by the nifH deletion strain Azotobacter vinelandii DJ1165 (Delta(nifH) MoFe protein)

47 the cytochrome bd terminating branch of the Azotobacter vinelandii electron transport chain, the pur

48 bacter sphaeroides has been purified from an Azotobacter vinelandii expression system; its interactio

49 ne of the Cys(39)-X-X-Cys(42)-X-X-Cys(45) of Azotobacter vinelandii FdI, which coordinates its [4Fe-4

50 ine (D39N) in the nucleotide binding site of Azotobacter vinelandii Fe protein by site-directed mutag

51 8 (located near the [4Fe-4S] cluster) of the Azotobacter vinelandii Fe protein were changed by means

52 ial (E(0)') of the [4Fe-4S](2+/+) cluster of Azotobacter vinelandii ferredoxin I (AvFdI) and related

53 in the [3Fe-4S]+/0 location of native (7Fe) Azotobacter vinelandii ferredoxin I (AvFdI) by providing

54 Azotobacter vinelandii ferredoxin I (AvFdI) is a seven-i

55 Azotobacter vinelandii ferredoxin I (AvFdI) is one membe

56 The crystal structure of Azotobacter vinelandii ferredoxin I (FdI) at 100 K has b

57 The [4Fe-4S](2+/+) cluster of Azotobacter vinelandii ferredoxin I (FdI) has an unusual

58 d a 3Fe to 4Fe cluster conversion variant of Azotobacter vinelandii ferredoxin I (FdI) in which the s

59 fication of site-directed mutant variants of Azotobacter vinelandii ferredoxin I (FdI), a pink protei

60 In Azotobacter vinelandii ferredoxin I, reduction of a buri

61 Here we have produced and isolated Azotobacter vinelandii FeS II and have determined its th

62 A tungsten-tolerant mutant strain (CA6) of Azotobacter vinelandii first described in 1980 has been

63 Azotobacter vinelandii flavodoxin hydroquinone (FldHQ) i

64 Using reduced Azotobacter vinelandii flavoprotein (AvFlpH(2)), a possi

65 ifH mutants in the nitrogen-fixing bacterium Azotobacter vinelandii for mutants that acquired NifM in

66 The nifE and nifN gene products from Azotobacter vinelandii form an alpha2beta2 tetramer (Nif

67 uctases and most similar to the structure of Azotobacter vinelandii FPR and Escherichia coli flavodox

68 mation of the Fe protein of nitrogenase from Azotobacter vinelandii has been examined in solution by

69 The nifZ gene product (NifZ) of Azotobacter vinelandii has been implicated in MoFe prote

70 ructure of the nitrogenase MoFe-protein from Azotobacter vinelandii has been refined to 2.0 A resolut

71 The role of the Azotobacter vinelandii HscA/HscB cochaperone system in I

72 ffects of flagella on deposition dynamics of Azotobacter vinelandii in porous media, independent of m

73 ructure of the nitrogenase iron protein from Azotobacter vinelandii in the all-ferrous [4Fe-4S](0) fo

74 oplasmic molybdate-binding protein ModG from Azotobacter vinelandii in two different crystal forms ha

75 n that an E146D site-directed variant of the Azotobacter vinelandii iron protein (Fe protein) is spec

76 Azotobacter vinelandii is a soil bacterium related to th

77 Azotobacter vinelandii is a terrestrial diazotroph well

78 d apodinitrogenase (apodinitrogenase 2) from Azotobacter vinelandii is an alpha2beta2delta2 hexamer.

79 tructure of the dinitrogenase reductase from Azotobacter vinelandii is known.

80 in the presence of a plasmid that harbors an Azotobacter vinelandii isc operon, which is involved in

81 Altered MoFe proteins of Azotobacter vinelandii Mo-nitrogenase, with amino acid s

82 we present kinetic parameters for an altered Azotobacter vinelandii MoFe protein for which the alphaG

83 cupying the S2B site of FeMo-cofactor in the Azotobacter vinelandii MoFe-protein, a position that was

84 taArg401Glu) in this patch were generated in Azotobacter vinelandii MoFeP.

85 ction was carried out with the extract of an Azotobacter vinelandii mutant lacking apodinitrogenase.

86 hown previously to accumulate on VnfX in the Azotobacter vinelandii mutant strain CA11.1 (DeltanifHDK

87 Certain Azotobacter vinelandii mutant strains unable to synthesi

88 The Azotobacter vinelandii NafY protein (nitrogenase accesso

89 Azotobacter vinelandii NIFL is a nitrogen fixation-speci

90 The Azotobacter vinelandii NIFL regulatory flavoprotein resp

91 percent) with Halobacterium salinarium Bat, Azotobacter vinelandii NIFL, Neurospora crassa White Col

92 Heterologous expression of Azotobacter vinelandii nifS from a compatible plasmid in

93 t can catalyze to the protein encoded by the Azotobacter vinelandii nifS gene.

94 ystem consisting of l-selenocysteine and the Azotobacter vinelandii NifS protein can replace selenide

95 The Azotobacter vinelandii nifW gene, under control of the n

96 ecursors and their transfer between purified Azotobacter vinelandii NifX and NifEN proteins was studi

97 that the combination of the MoFe protein of Azotobacter vinelandii nitrogenase (Av1) with the Fe pro

98 rated in the fully reduced Fe protein of the Azotobacter vinelandii nitrogenase complex.

99 Azotobacter vinelandii nitrogenase Fe protein (Av2) prov

100 the effects of MgATP or MgADP binding to the Azotobacter vinelandii nitrogenase Fe protein on the pro

101 dence for primary electron transfer from the Azotobacter vinelandii nitrogenase Fe protein to the MoF

102 present study, the crystal structure of the Azotobacter vinelandii nitrogenase Fe protein variant ha

103 Recent work on the Fe protein of Azotobacter vinelandii nitrogenase has demonstrated the

104 An Azotobacter vinelandii nitrogenase iron protein mutant h

105 Wild-type and three altered Azotobacter vinelandii nitrogenase MoFe proteins, with s

106 iosynthesis of the FeMo cofactor (FeMoco) of Azotobacter vinelandii nitrogenase presumably starts wit

107 nt EPR signals, designated 1b and 1c, during Azotobacter vinelandii nitrogenase turnover at 23 degree

108 on the FeMo cofactor of the MoFe protein of Azotobacter vinelandii nitrogenase were probed using C(2

109 fully reduced cluster of the iron protein of Azotobacter vinelandii nitrogenase, including a common S

110 the [4Fe-4S](+) cluster in the Fe protein of Azotobacter vinelandii nitrogenase, which exists in two

111 The bacterium Azotobacter vinelandii produces a family of seven secret

112 Previous studies have shown that Azotobacter vinelandii produces at least two [Fe-S] clus

113 he extracellular alginate epimerase AlgE4 of Azotobacter vinelandii provides a structural rationale f

114 h; (ii) enzymatic, in which NifS protein of Azotobacter vinelandii regenerated active Fe-SoxR in as

115 the MoFe protein isolated from the bacterium Azotobacter vinelandii resulted in an inactive, nondisso

116 resented 1.6 A X-ray structure of MoSto from Azotobacter vinelandii reveals various discrete polyoxom

117 The NifL regulatory protein from Azotobacter vinelandii senses the oxygen status of the c

118 instead of alpha-195(His)) from a mutant of Azotobacter vinelandii show, contrary to an earlier repo

119 (apodinitrogenase 2) has been purified from Azotobacter vinelandii strain CA117.30 (DeltanifKDB), an

120 The Azotobacter vinelandii strain expressing an E146D Fe pro

121 The Azotobacter vinelandii strain expressing M156C is unable

122 Azotobacter vinelandii strains lacking the nitrogenase-p

123 oped for the isolation of a mutant strain of Azotobacter vinelandii that exhibits in vivo nitrogenase

124 To determine whether in Azotobacter vinelandii the PII protein influences the re

125 ure of the FAD-bound PAS domain of NifL from Azotobacter vinelandii to 1.04 A resolution.

126 ture of the ubiquitous N(2) fixing bacterium Azotobacter vinelandii under Mo replete and Mo limiting

127 nitrogenase negative phenotype exhibited by Azotobacter vinelandii UW97.

128 zation of the vanadium iron (VFe) protein of Azotobacter vinelandii V-nitrogenase has been focused on

129 interstitial carbide in the Fe-V cofactor of Azotobacter vinelandii vanadium nitrogenase.

130 nitrogenase (lacking the FeMo cofactor) from Azotobacter vinelandii was extracted from the alternativ

131 The nifV gene product (NifV) from Azotobacter vinelandii was recombinantly expressed at hi

132 ification of a novel ferredoxin (FdIII) from Azotobacter vinelandii which brings to 12 the number of

133 A gene from Azotobacter vinelandii whose product exhibits primary se

134 vestigate mRNA produced by mutant strains of Azotobacter vinelandii with defined deletions in the nif

135 The ability of Azotobacter vinelandii(Nif)IscA to bind Fe has been inve

136 he mechanism of [4Fe-4S] cluster assembly on Azotobacter vinelandii(Nif)IscA, and the ability of (Nif

137 tituting Fe-S clusters with the NifS enzyme (Azotobacter vinelandii) were unsuccessful.

138 ifD product (with the exception of vnfE from Azotobacter vinelandii), suggesting that a gene duplicat

139 lting from nifH and nifB deletion strains of Azotobacter vinelandii, a novel [Fe-S] cluster is identi

140 ification of the V nitrogenase proteins from Azotobacter vinelandii, an increase in resolution was ob

141 Azotobacter vinelandii, an obligate aerobe, fixes nitrog

142 th ptsP orthologs of Pseudomonas aeruginosa, Azotobacter vinelandii, and Escherichia coli, with nearl

143 genes (aefA from Escherichia coli, pstP from Azotobacter vinelandii, and mtrR from Neisseria gonorrho

144 two phylogenetically distinct nitrogenases (Azotobacter vinelandii, Av, and Clostridium pasteurianum

145 on-sulfur cluster biosynthesis proteins from Azotobacter vinelandii, contains one [4Fe-4S](2+) cluste

146 In Azotobacter vinelandii, deletion of the fdxA gene that e

147 In Azotobacter vinelandii, deletion of the fdxA gene, which

148 An IscA homologue within the nif regulon of Azotobacter vinelandii, designated (Nif)IscA, was expres

149 The obligate aerobic diazotroph, Azotobacter vinelandii, employs a multitude of protectiv

150 In Azotobacter vinelandii, expression of nifA, encoding the

151 In Azotobacter vinelandii, expression of the three differen

152 have now shown that in Escherichia coli and Azotobacter vinelandii, GlnK binds to the membrane in an

153 3), was identified in ModE and homologs from Azotobacter vinelandii, Haemophilus influenzae, Rhodobac

154 -tune regulation of nitrogenase synthesis in Azotobacter vinelandii, is a potential target for PII-me

155 The hydrogenase in Azotobacter vinelandii, like other membrane-bound [NiFe]

156 In Azotobacter vinelandii, NafY (also known as gamma protei

157 more susceptible than nitrogen fixing (i.e., Azotobacter vinelandii, Rhizobium etli, and Azospirillum

158 strains that have a V-nitrogenase, including Azotobacter vinelandii, Rhodopseudomonas palustris, and

159 h were produced in certain mutant strains of Azotobacter vinelandii, showed that the N coordination t

160 In Azotobacter vinelandii, the anfHDGK operon encodes the s

161 In Azotobacter vinelandii, the NifEN complex, the site for

162 onstrated using a monomeric form of IDH from Azotobacter vinelandii, which can be shown to gain the s

163 of nitrogenase Fe protein from R. rubrum or Azotobacter vinelandii.

164 eported for the closely related protein from Azotobacter vinelandii.

165 MoFe protein) from a nifB-deletion mutant of Azotobacter vinelandii.

166 e bd quinol oxidases of Escherichia coli and Azotobacter vinelandii.

167 rome bd from its counterparts in E. coli and Azotobacter vinelandii.

168 nvestigated for alginic acid biosynthesis in Azotobacter vinelandii.

169 genes that are similar to the vnfEN genes of Azotobacter vinelandii.

170 on of a His-tagged NifEN-B fusion protein of Azotobacter vinelandii.

171 fixation in the model diazotrophic bacterium Azotobacter vinelandii.

172 NifB-co while bound to the NifX protein from Azotobacter vinelandii.

173 mologous to oxidases in Escherichia coli and Azotobacter vinelandii.

174 cofactor of the molybdenum nitrogenase from Azotobacter vinelandii.

175 f rnf genes in the nitrogen-fixing bacterium Azotobacter vinelandii.

176 d the extracellular mannuronan epimerases of Azotobacter vinelandii.

177 ement with the measured value of -0.042 V in Azotobacter vinelandii; and (3) average Mossbauer isomer

178 structure of the PAS-FAD domain in NifL from Azotobacter vinlandii.