ライフサイエンスコーパス: Azotobacter vinelandii

1 of nitrogenase Fe protein from R. rubrum or Azotobacter vinelandii.

2 eported for the closely related protein from Azotobacter vinelandii.

3 MoFe protein) from a nifB-deletion mutant of Azotobacter vinelandii.

4 e bd quinol oxidases of Escherichia coli and Azotobacter vinelandii.

5 rome bd from its counterparts in E. coli and Azotobacter vinelandii.

6 nvestigated for alginic acid biosynthesis in Azotobacter vinelandii.

7 genes that are similar to the vnfEN genes of Azotobacter vinelandii.

8 on of a His-tagged NifEN-B fusion protein of Azotobacter vinelandii.

9 fixation in the model diazotrophic bacterium Azotobacter vinelandii.

10 NifB-co while bound to the NifX protein from Azotobacter vinelandii.

11 cofactor of the molybdenum nitrogenase from Azotobacter vinelandii.

12 mologous to oxidases in Escherichia coli and Azotobacter vinelandii.

13 f rnf genes in the nitrogen-fixing bacterium Azotobacter vinelandii.

14 d the extracellular mannuronan epimerases of Azotobacter vinelandii.

15 The refined structure of reduced Azotobacter vinelandii 7Fe ferredoxin FdI at 100 K and 1

16 he ferricyanide-oxidized [4Fe-4S] cluster in Azotobacter vinelandii 7Fe ferredoxin, the spectroscopic

17 lting from nifH and nifB deletion strains of Azotobacter vinelandii, a novel [Fe-S] cluster is identi

18 ification of the V nitrogenase proteins from Azotobacter vinelandii, an increase in resolution was ob

19 Azotobacter vinelandii, an obligate aerobe, fixes nitrog

20 in vitro analysis of two CooA homologs from Azotobacter vinelandii and Carboxydothermus hydrogenofor

21 is presented that nitrogenases isolated from Azotobacter vinelandii and Clostridium pasteurianum can

22 , crystal structures of the Fe-proteins from Azotobacter vinelandii and Clostridium pasteurianum have

23 s-NifEN protein from a DeltanifHDK strain of Azotobacter vinelandii and have found that the amounts o

24 and Pseudomonas putidabut not as strictly in Azotobacter vinelandii and not at all in P. aeruginosa.

25 lity we analyzed the genome of the bacterium Azotobacter vinelandii and show that genes that code for

26 Unlike glnD mutations in Azotobacter vinelandii and some other bacteria, glnD del

27 oxidoreductase was cloned and sequenced from Azotobacter vinelandii and termed the dsbA locus.

28 th ptsP orthologs of Pseudomonas aeruginosa, Azotobacter vinelandii, and Escherichia coli, with nearl

29 genes (aefA from Escherichia coli, pstP from Azotobacter vinelandii, and mtrR from Neisseria gonorrho

30 ement with the measured value of -0.042 V in Azotobacter vinelandii; and (3) average Mossbauer isomer

31 The NifS and NifU proteins from Azotobacter vinelandii are required for the full activat

32 We used Fe protein from Azotobacter vinelandii as the substrate to assess the ac

33 he structure of the precursor-bound NifEN of Azotobacter vinelandii at 2.6 angstrom resolution.

34 The properties of CO-inhibited Azotobacter vinelandii (Av) Mo-nitrogenase (N2ase) have

35 lysis of the two-component protein system of Azotobacter vinelandii (Av) nitrogenase is reported.

36 n this paper, we compare the interactions of Azotobacter vinelandii (Av) nitrogenase with two diazene

37 two phylogenetically distinct nitrogenases (Azotobacter vinelandii, Av, and Clostridium pasteurianum

38 The nitrogenase VFe protein of Azotobacter vinelandii (Av1') has been shown to exist in

39 Azotobacter vinelandii bacterioferritin (AvBF) containin

40 nite-reduced (at pH 8.0) forms of the native Azotobacter vinelandii bacterioferritin to 2.7 and 2.0 A

41 The NifA protein from Azotobacter vinelandii belongs to a family of enhancer b

42 transcription of nitrogen fixation genes in Azotobacter vinelandii by modulating the activity of the

43 em for the controlled expression of genes in Azotobacter vinelandii by using genomic fusions to the s

44 itrogen fixation by the free-living organism Azotobacter vinelandii can occur through the activity of

45 The vanadium (V)-nitrogenase of Azotobacter vinelandii catalyses the in vitro conversion

46 eMo-co synthesis system is not required when Azotobacter vinelandii cell-free extract is prepared in

47 The vnf-encoded nitrogenase from Azotobacter vinelandii contains an iron-vanadium cofacto

48 The nitrogen-fixing organism Azotobacter vinelandii contains at least two systems tha

49 The NifL regulatory protein from Azotobacter vinelandii contains tandem PAS domains, the

50 on-sulfur cluster biosynthesis proteins from Azotobacter vinelandii, contains one [4Fe-4S](2+) cluste

51 The Azotobacter vinelandii cytochrome c5 gene (termed cycB)

52 xtracts from two different mutant strains of Azotobacter vinelandii defective in the biosynthesis of

53 e NifS protein was purified from a strain of Azotobacter vinelandii deleted for the nifS gene.

54 In Azotobacter vinelandii, deletion of the fdxA gene that e

55 In Azotobacter vinelandii, deletion of the fdxA gene, which

56 of this complex generated with proteins from Azotobacter vinelandii (designated the L127Delta-Av2-Av1

57 An IscA homologue within the nif regulon of Azotobacter vinelandii, designated (Nif)IscA, was expres

58 e structure of the catalytic domain from the Azotobacter vinelandii dihydrolipoamide acetyltransferas

59 rotein expressed by the nifH deletion strain Azotobacter vinelandii DJ1165 (Delta(nifH) MoFe protein)

60 Here, a mutant strain of Azotobacter vinelandii (DJ1193) was used to facilitate t

61 the cytochrome bd terminating branch of the Azotobacter vinelandii electron transport chain, the pur

62 The obligate aerobic diazotroph, Azotobacter vinelandii, employs a multitude of protectiv

63 bacter sphaeroides has been purified from an Azotobacter vinelandii expression system; its interactio

64 In Azotobacter vinelandii, expression of nifA, encoding the

65 In Azotobacter vinelandii, expression of the three differen

66 ne of the Cys(39)-X-X-Cys(42)-X-X-Cys(45) of Azotobacter vinelandii FdI, which coordinates its [4Fe-4

67 ine (D39N) in the nucleotide binding site of Azotobacter vinelandii Fe protein by site-directed mutag

68 8 (located near the [4Fe-4S] cluster) of the Azotobacter vinelandii Fe protein were changed by means

69 In this work, cluster transfer from Azotobacter vinelandii [Fe(2)S(2)](2+) cluster-bound Isc

70 e, it is shown that production of the intact Azotobacter vinelandii [Fe-S] cluster biosynthetic machi

71 ial (E(0)') of the [4Fe-4S](2+/+) cluster of Azotobacter vinelandii ferredoxin I (AvFdI) and related

72 in the [3Fe-4S]+/0 location of native (7Fe) Azotobacter vinelandii ferredoxin I (AvFdI) by providing

73 Azotobacter vinelandii ferredoxin I (AvFdI) is a seven-i

74 Azotobacter vinelandii ferredoxin I (AvFdI) is one membe

75 The crystal structure of Azotobacter vinelandii ferredoxin I (FdI) at 100 K has b

76 The [4Fe-4S](2+/+) cluster of Azotobacter vinelandii ferredoxin I (FdI) has an unusual

77 d a 3Fe to 4Fe cluster conversion variant of Azotobacter vinelandii ferredoxin I (FdI) in which the s

78 fication of site-directed mutant variants of Azotobacter vinelandii ferredoxin I (FdI), a pink protei

79 In Azotobacter vinelandii ferredoxin I, reduction of a buri

80 Here we have produced and isolated Azotobacter vinelandii FeS II and have determined its th

81 A tungsten-tolerant mutant strain (CA6) of Azotobacter vinelandii first described in 1980 has been

82 Azotobacter vinelandii flavodoxin hydroquinone (FldHQ) i

83 Using reduced Azotobacter vinelandii flavoprotein (AvFlpH(2)), a possi

84 ifH mutants in the nitrogen-fixing bacterium Azotobacter vinelandii for mutants that acquired NifM in

85 The nifE and nifN gene products from Azotobacter vinelandii form an alpha2beta2 tetramer (Nif

86 uctases and most similar to the structure of Azotobacter vinelandii FPR and Escherichia coli flavodox

87 have now shown that in Escherichia coli and Azotobacter vinelandii, GlnK binds to the membrane in an

88 3), was identified in ModE and homologs from Azotobacter vinelandii, Haemophilus influenzae, Rhodobac

89 mation of the Fe protein of nitrogenase from Azotobacter vinelandii has been examined in solution by

90 The nifZ gene product (NifZ) of Azotobacter vinelandii has been implicated in MoFe prote

91 ructure of the nitrogenase MoFe-protein from Azotobacter vinelandii has been refined to 2.0 A resolut

92 The role of the Azotobacter vinelandii HscA/HscB cochaperone system in I

93 ffects of flagella on deposition dynamics of Azotobacter vinelandii in porous media, independent of m

94 ructure of the nitrogenase iron protein from Azotobacter vinelandii in the all-ferrous [4Fe-4S](0) fo

95 oplasmic molybdate-binding protein ModG from Azotobacter vinelandii in two different crystal forms ha

96 n that an E146D site-directed variant of the Azotobacter vinelandii iron protein (Fe protein) is spec

97 Azotobacter vinelandii is a soil bacterium related to th

98 Azotobacter vinelandii is a terrestrial diazotroph well

99 d apodinitrogenase (apodinitrogenase 2) from Azotobacter vinelandii is an alpha2beta2delta2 hexamer.

100 tructure of the dinitrogenase reductase from Azotobacter vinelandii is known.

101 -tune regulation of nitrogenase synthesis in Azotobacter vinelandii, is a potential target for PII-me

102 in the presence of a plasmid that harbors an Azotobacter vinelandii isc operon, which is involved in

103 The hydrogenase in Azotobacter vinelandii, like other membrane-bound [NiFe]

104 Altered MoFe proteins of Azotobacter vinelandii Mo-nitrogenase, with amino acid s

105 we present kinetic parameters for an altered Azotobacter vinelandii MoFe protein for which the alphaG

106 cupying the S2B site of FeMo-cofactor in the Azotobacter vinelandii MoFe-protein, a position that was

107 taArg401Glu) in this patch were generated in Azotobacter vinelandii MoFeP.

108 ction was carried out with the extract of an Azotobacter vinelandii mutant lacking apodinitrogenase.

109 hown previously to accumulate on VnfX in the Azotobacter vinelandii mutant strain CA11.1 (DeltanifHDK

110 Certain Azotobacter vinelandii mutant strains unable to synthesi

111 The Azotobacter vinelandii NafY protein (nitrogenase accesso

112 In Azotobacter vinelandii, NafY (also known as gamma protei

113 The ability of Azotobacter vinelandii(Nif)IscA to bind Fe has been inve

114 he mechanism of [4Fe-4S] cluster assembly on Azotobacter vinelandii(Nif)IscA, and the ability of (Nif

115 Azotobacter vinelandii NIFL is a nitrogen fixation-speci

116 The Azotobacter vinelandii NIFL regulatory flavoprotein resp

117 percent) with Halobacterium salinarium Bat, Azotobacter vinelandii NIFL, Neurospora crassa White Col

118 Heterologous expression of Azotobacter vinelandii nifS from a compatible plasmid in

119 t can catalyze to the protein encoded by the Azotobacter vinelandii nifS gene.

120 ystem consisting of l-selenocysteine and the Azotobacter vinelandii NifS protein can replace selenide

121 The Azotobacter vinelandii nifW gene, under control of the n

122 ecursors and their transfer between purified Azotobacter vinelandii NifX and NifEN proteins was studi

123 that the combination of the MoFe protein of Azotobacter vinelandii nitrogenase (Av1) with the Fe pro

124 rated in the fully reduced Fe protein of the Azotobacter vinelandii nitrogenase complex.

125 Azotobacter vinelandii nitrogenase Fe protein (Av2) prov

126 the effects of MgATP or MgADP binding to the Azotobacter vinelandii nitrogenase Fe protein on the pro

127 dence for primary electron transfer from the Azotobacter vinelandii nitrogenase Fe protein to the MoF

128 present study, the crystal structure of the Azotobacter vinelandii nitrogenase Fe protein variant ha

129 Recent work on the Fe protein of Azotobacter vinelandii nitrogenase has demonstrated the

130 An Azotobacter vinelandii nitrogenase iron protein mutant h

131 Wild-type and three altered Azotobacter vinelandii nitrogenase MoFe proteins, with s

132 iosynthesis of the FeMo cofactor (FeMoco) of Azotobacter vinelandii nitrogenase presumably starts wit

133 nt EPR signals, designated 1b and 1c, during Azotobacter vinelandii nitrogenase turnover at 23 degree

134 on the FeMo cofactor of the MoFe protein of Azotobacter vinelandii nitrogenase were probed using C(2

135 fully reduced cluster of the iron protein of Azotobacter vinelandii nitrogenase, including a common S

136 the [4Fe-4S](+) cluster in the Fe protein of Azotobacter vinelandii nitrogenase, which exists in two

137 The bacterium Azotobacter vinelandii produces a family of seven secret

138 Previous studies have shown that Azotobacter vinelandii produces at least two [Fe-S] clus

139 he extracellular alginate epimerase AlgE4 of Azotobacter vinelandii provides a structural rationale f

140 h; (ii) enzymatic, in which NifS protein of Azotobacter vinelandii regenerated active Fe-SoxR in as

141 the MoFe protein isolated from the bacterium Azotobacter vinelandii resulted in an inactive, nondisso

142 resented 1.6 A X-ray structure of MoSto from Azotobacter vinelandii reveals various discrete polyoxom

143 more susceptible than nitrogen fixing (i.e., Azotobacter vinelandii, Rhizobium etli, and Azospirillum

144 strains that have a V-nitrogenase, including Azotobacter vinelandii, Rhodopseudomonas palustris, and

145 The NifL regulatory protein from Azotobacter vinelandii senses the oxygen status of the c

146 instead of alpha-195(His)) from a mutant of Azotobacter vinelandii show, contrary to an earlier repo

147 h were produced in certain mutant strains of Azotobacter vinelandii, showed that the N coordination t

148 (apodinitrogenase 2) has been purified from Azotobacter vinelandii strain CA117.30 (DeltanifKDB), an

149 The Azotobacter vinelandii strain expressing an E146D Fe pro

150 The Azotobacter vinelandii strain expressing M156C is unable

151 Azotobacter vinelandii strains lacking the nitrogenase-p

152 ifD product (with the exception of vnfE from Azotobacter vinelandii), suggesting that a gene duplicat

153 oped for the isolation of a mutant strain of Azotobacter vinelandii that exhibits in vivo nitrogenase

154 To determine whether in Azotobacter vinelandii the PII protein influences the re

155 of a new group II intron from the bacterium Azotobacter vinelandii (the AV intron).

156 In Azotobacter vinelandii, the anfHDGK operon encodes the s

157 In Azotobacter vinelandii, the NifEN complex, the site for

158 ure of the FAD-bound PAS domain of NifL from Azotobacter vinelandii to 1.04 A resolution.

159 ture of the ubiquitous N(2) fixing bacterium Azotobacter vinelandii under Mo replete and Mo limiting

160 nitrogenase negative phenotype exhibited by Azotobacter vinelandii UW97.

161 zation of the vanadium iron (VFe) protein of Azotobacter vinelandii V-nitrogenase has been focused on

162 interstitial carbide in the Fe-V cofactor of Azotobacter vinelandii vanadium nitrogenase.

163 nitrogenase (lacking the FeMo cofactor) from Azotobacter vinelandii was extracted from the alternativ

164 The nifV gene product (NifV) from Azotobacter vinelandii was recombinantly expressed at hi

165 tituting Fe-S clusters with the NifS enzyme (Azotobacter vinelandii) were unsuccessful.

166 ification of a novel ferredoxin (FdIII) from Azotobacter vinelandii which brings to 12 the number of

167 onstrated using a monomeric form of IDH from Azotobacter vinelandii, which can be shown to gain the s

168 A gene from Azotobacter vinelandii whose product exhibits primary se

169 vestigate mRNA produced by mutant strains of Azotobacter vinelandii with defined deletions in the nif