ライフサイエンスコーパス: B. abortus

1 B. abortus 2308 persisted within these cathepsin D(-), L

2 B. abortus ahpC and katE mutants exhibit wild-type virul

3 B. abortus and B. abortus DNA-stimulated IL-12 productio

4 B. abortus cell-cycle progression is coordinated with in

5 B. abortus cydB::Tn5 strains exhibited heightened sensit

6 B. abortus given without OVA did not diminish the subseq

7 B. abortus O antigen appears to be essential for extra-

8 B. abortus RA1 was attenuated for survival in mice.

9 B. abortus was shown to rapidly increase the expression

10 B. abortus, B. melitensis, B. suis, and B. canis produce

11 In the absence of IL-10, B. abortus LPS induced strong IFN-gamma responses, but I

12 quence, primers were designed to construct a B. abortus deletion mutant.

13 In the mouse model, a B. abortus virB mutant was initially able to colonize th

14 hypersensitive to UV damage, surprisingly a B. abortus recA null mutant conferred only modest sensit

15 was uncovered when it was determined that a B. abortus dhbC mutant (BHB1) defective in 2,3-DHBA prod

16 and cytotoxic T-cell epitopes to B. abortus (B. abortus-MN 18-mer).

17 acellular replication, the numbers of acidic B. abortus 2308 BCP decreased while remaining cathepsin

18 In addition, B. abortus activates human CD4+ and CD8+ cells to secret

19 strain RB51 or its vaccine efficacy against B. abortus 2308 challenge.

20 P, immunoreactivity with Ab prepared against B. abortus group Ags, immunolabeling of whole cells, and

21 cinated cattle did not contain agglutinating B. abortus antibody in the tube agglutination test for b

22 al concentration of hydrogen peroxide allows B. abortus to adapt so as to survive subsequent exposure

23 Although B. abortus-activated T cells actively secreted the pro-o

24 Although B. abortus-infected glial cells secreted IL-1beta and TN

25 enes, we searched the Brucella suis 1330 and B. abortus 2308 genomes for genes with an upstream virB

26 smooth, virulent Brucella melitensis 16M and B. abortus 2308, rough wboA mutants VTRM1, RA1, and WRR5

27 However, only B. abortus and B. abortus DNA induced high levels of IFN-gamma and IL-1

28 Th1-like cytokine response to B. abortus and B. abortus DNA, which was confirmed by using neutralizin

29 B. abortus and B. abortus DNA-stimulated IL-12 production was maximal b

30 strate that the cross talk of LX-2 cells and B. abortus induces autophagy and fibrosis with concomita

31 The lipid A molecules of S. meliloti and B. abortus are unusually modified with a very-long-chain

32 Since both the S. meliloti and B. abortus bacA mutants have an increased resistance to

33 he chronic infection of both S. meliloti and B. abortus.

34 bacterial pathogens Brucella melitensis and B. abortus.

35 sion in T cells separated from monocytes and B. abortus by a transwell membrane.

36 tructures of the enzyme from B. subtilis and B. abortus and describe the C teminus structure which ac

37 as B. abortus strain RB51, 57 identified as B. abortus biovar 1, 15 identified as B. abortus bv.2, 1

38 entified as B. abortus bv.2, 1 identified as B. abortus bv.2 (M antigen dominant), 7 identified as B.

39 ied as B. abortus biovar 1, 15 identified as B. abortus bv.2, 1 identified as B. abortus bv.2 (M anti

40 s bv.2 (M antigen dominant), 7 identified as B. abortus bv.4, and 22 identified as B. abortus S2308 a

41 This included 120 isolates identified as B. abortus S19, 9 identified as B. abortus strain RB51,

42 ied as B. abortus bv.4, and 22 identified as B. abortus S2308 and isolated from experimentally infect

43 dentified as B. abortus S19, 9 identified as B. abortus strain RB51, 57 identified as B. abortus biov

44 -cytotoxic to human host cells and attenuate B. abortus replication in the intracellular niche.

45 To identify in vivo interactions between B. abortus and the host that lead to persistent infectio

46 th stress survival and the interface between B. abortus and the host immune system.

47 dozen genes, the genetic complements of both B. abortus strains are identical, whereas the three spec

48 Animals infected with both B. abortus and B. pahangi showed increased IFN-gamma and

49 ivation and further secretion of IL-1beta by B. abortus in macrophages.

50 T4SS, we compared host responses elicited by B. abortus with those of an isogenic mutant in the virB

51 indicate that activated T cells, elicited by B. abortus-infected macrophages and influenced by the in

52 chanisms of liver immune response induced by B. abortus-infected hepatic stellate cells.

53 d maintenance of chronic spleen infection by B. abortus in mice, experimental infection of pregnant c

54 Infection of macrophages by B. abortus was stimulated by light in the wild type but

55 to evasion of adaptive immune mechanisms by B. abortus.

56 innate immunity of the CNS set in motion by B. abortus contributes to the activation of the blood-br

57 as restored by an intact copy of cydAB or by B. abortus genes encoding the oxidative radical-scavengi

58 The biological activity of IL-12 secreted by B. abortus-stimulated monocytes was demonstrated by its

59 Here a highly conserved B. abortus homolog of the R. meliloti bacA gene, which e

60 When used to infect macrophages, DeltaznuA B. abortus showed minimal growth.

61 Further study with DeltaznuA B. abortus showed that its virulence in BALB/c mice was

62 catalase deletion mutations in two different B. abortus strains and used two-dimensional gel analysis

63 pression of proinflammatory cytokines during B. abortus infection.

64 sed interleukin-12 p40 mRNA following either B. abortus or B. abortus-OVA administration.

65 In extravillous trophoblasts (EVTs), B. abortus and B. suis replicated within single-membrane

66 nant O. anthropi strain 49237SOD, expressing B. abortus Cu,Zn SOD, provided protection against virule

67 y, the response of macrophages 4 h following B. abortus infection was analyzed to identify early intr

68 R/C, and Pr phages that are lytic mainly for B. abortus, Brucella melitensis and Brucella suis; Group

69 assay was 800 CFU equivalents/ml of milk for B. abortus and as low as 4 CFU equivalents per ml of mil

70 roteins represent important iron sources for B. abortus 2308 during its residence in the mammalian ho

71 utant, designated MEK2, was constructed from B. abortus 2308 by gene replacement, and the sodC mutant

72 an isogenic ftrA mutant was constructed from B. abortus 2308.

73 EC was dependent on IL-1beta because CS from B. abortus-infected astrocytes and microglia deficient i

74 ysis of an isogenic bhuA mutant derived from B. abortus 2308 verified that there is a link between Bh

75 NHS than OPS-deficient strains derived from B. abortus 2308, (ii) both the classical and the MBL-med

76 protein was a group 3 protein distinct from B. abortus Omp25.

77 l X-ray crystal structure of the enzyme from B. abortus has been solved and refined at 2.7 A resoluti

78 lt from 12 pentameric units, the enzyme from B. abortus is pentameric in its crystalline form.

79 B. abortus and lipopolysaccharide (LPS) from B. abortus can induce IL-12 p40 mRNA expression and prot

80 t mRNAs, indicating that the AbcR sRNAs from B. abortus 2308 perform redundant regulatory functions.

81 In contrast, supernatants from B. abortus-infected hepatocytes and monocytes induce MMP

82 . abortus, the capacity of supernatants from B. abortus-infected hepatocytes and monocytes to induce

83 transposon mutagenesis was used to generate B. abortus rough mutants defective in O-antigen presenta

84 In order to understand how B. abortus copes with the conditions during intracellula

85 cking either NADPH oxidase or iNOS, however, B. abortus infected and persisted to the same extent as

86 We identified B. abortus genes involved in chronic infection, by asses

87 d immunity, it was important to determine if B. abortus conjugates would also act as a carrier to ind

88 In B. abortus 2308, MucR regulates a wide variety of genes

89 Maximum expression of bhuA in B. abortus 2308 is observed during stationary phase when

90 the ccrM gene results in striking changes in B. abortus morphology, DNA replication, and growth in mu

91 olysis is a unique feature of GSR control in B. abortus.

92 Allelic exchange of genomic DNA in B. abortus 2308 mediated by electroporation of pJM6 prod

93 Because CcrM is essential in B. abortus and increased ccrM copy number attenuates sur

94 suggest that only one copy of galE exists in B. abortus 2308.

95 iron needed to repress dhbCEBA expression in B. abortus 2308 was also greater when this strain was cu

96 ht serve as virulence or survival factors in B. abortus infections.

97 proteolysis is a novel regulatory feature in B. abortus that ensures proper control of GSR transcript

98 We propose that galE in B. abortus evolved by lateral transfer from other animal

99 product, the expression of the mntH gene in B. abortus 2308 is repressed by Mn(2+), but not Fe(2+),

100 ted AbcR1 and AbcR2) have been identified in B. abortus 2308.

101 Transcription of the ftrA gene is induced in B. abortus 2308 in response to iron deprivation and expo

102 on; its transcription is strongly induced in B. abortus by various stressors encountered by the bacte

103 epair and resistance to oxidative killing in B. abortus 2308, XthA-1 is not required for wild-type vi

104 , the transcriptional start site for mntH in B. abortus 2308 was determined by primer extension analy

105 present study the genes regulated by MucR in B. abortus have been elucidated using microarray analysi

106 A znuA knockout mutation in B. abortus 2308 (DeltaznuA) was constructed and found to

107 detectable levels of Irr were found only in B. abortus 2308 cells by Western blot analysis following

108 hydroperoxide, but not hydrogen peroxide, in B. abortus 2308 and that OhrR represses the transcriptio

109 A protein, DNA repair, and SOS regulation in B. abortus.

110 -kDa protein, which was detected in vitro in B. abortus grown to stationary phase.

111 melitensis 16 M would be similar to znuA in B. abortus and questioned whether it may also be an impo

112 Thus, inactivated B. abortus can induce non-CD4+ cells to produce the cyto

113 nique to the alpha-proteobacteria, including B. abortus.

114 In samples from India, B. abortus shedding was identified in 86% of milk ring t

115 y constitutively and intracellularly induced B. abortus genes.

116 Twenty-six compounds inhibited B. abortus metabolism in axenic culture, thirteen of whi

117 n-cytotoxic compounds specifically inhibited B. abortus replication in the intracellular niche, which

118 An isogenic B. abortus bacA mutant exhibited decreased survival in m

119 Heat-killed B. abortus (HKBA) is a strong Th1 adjuvant and carrier.

120 Recently we demonstrated that heat-killed B. abortus (HKBa), a strong Th1 stimulus, conjugated to

121 hose seen for control particles (heat-killed B. abortus 2308, live Escherichia coli HB101, or latex b

122 set of genes that closely overlaps the known B. abortus GSR regulon.

123 in D(+) BCP was significantly lower for live B. abortus 2308-infected cells than for either Brucella

124 As a result of this finding, a new B. abortus htrA mutant, designated RWP11, was constructe

125 conjugated to ovalbumin (HKBA-OVA), but not B. abortus alone, can alter the antigen-specific cytokin

126 Furthermore, B. melitensis but not B. abortus nor B. suis interfered with the invasive capa

127 y of 480 known bioactive compounds for novel B. abortus anti-infectives.

128 etion of virB7 did not reduce the ability of B. abortus to persist in spleens of mice.

129 0, or virB11 markedly reduced the ability of B. abortus to persist in the spleens of mice at 8 weeks

130 he virB locus markedly reduce the ability of B. abortus to survive in cultured macrophages or to pers

131 All mutations reduced the ability of B. abortus to survive in J774A.1 mouse macrophage-like c

132 This proteomic analysis of B. abortus provides novel insights into the mechanisms u

133 in nasal swabs (2.9%) and milk (1.4%) and of B. abortus in milk (11.4%).

134 ntrols the cellular and infection biology of B. abortus.

135 revealed several deletions on chromosomes of B. abortus and B. melitensis that encoded proteins of va

136 unctions of Igh6(-/-) mice in the context of B. abortus infection.

137 Thus, proper control of B. abortus division site polarity is necessary for survi

138 dition of erythritol to low-iron cultures of B. abortus 2308 stimulated the production of 2,3-DHBA by

139 Here, we study the cell cycle of B. abortus at the single-cell level, in culture and duri

140 s to OVA/alum is secondary to the effects of B. abortus on expression of costimulatory molecules on T

141 Early trafficking events of B. abortus 2308-containing phagosomes (BCP) were indisti

142 fic targets (a region of the BCSP31K gene of B. abortus and a repeat-sequence region in the hsp65 gen

143 Here we present the genome of B. abortus 2308, the virulent prototype biovar 1 strain,

144 Identification of B. abortus genes that are activated following macrophage

145 A 640-kb inversion on chromosome II of B. abortus has been reported previously and is further d

146 efficacy of these molecules as inhibitors of B. abortus replication in the intracellular niche sugges

147 ek-old mice were given a single injection of B. abortus in the absence or presence of OVA, and at mat

148 e study the ability of a single injection of B. abortus to switch a Th2 to a Th1 response in immature

149 in parenchyma upon intracranial injection of B. abortus was diminished in the absence of Nod-like rec

150 ntrast, mice receiving a single injection of B. abortus-OVA at the age of 1 week, but not those injec

151 es were found to cross-react with the LPS of B. abortus, which shares an immunogenic epitope with B.

152 Transposon (Tn5) mutagenesis of B. abortus and the subsequent screening of mutants for s

153 n vivo immunizations with 10(8) organisms of B. abortus-MN 18-mer followed by in vitro stimulation wi

154 the htrA gene product to the pathogenesis of B. abortus infections.

155 mparison of the protein synthesis pattern of B. abortus during intracellular growth with those obtain

156 ent infection, we studied the persistence of B. abortus and an isogenic virB mutant deficient in the

157 ement for the T4SS to mediate persistence of B. abortus in the spleen.

158 by PPARgamma promotes chronic persistence of B. abortus within AAMs, and targeting this pathway may a

159 was also dispensable for the persistence of B. abortus, B. melitensis, and B. suis in mice up to 4 w

160 The in vitro phenotype of B. abortus RWP11 is consistent with the proposed functio

161 plays an important role in the physiology of B. abortus 2308, particularly during its intracellular s

162 with OVA/alum in the absence or presence of B. abortus.

163 Cu/Zn superoxide dismutase (SOD) protein of B. abortus in strain RB51.

164 findings indicate that the 14-kDa protein of B. abortus possesses lectin-like properties and is essen

165 A 14-kDa protein of B. abortus was previously identified to be immunogenic i

166 We demonstrate recognition of B. abortus antibodies through capture by fluorescent sil

167 ake was crucial for increased replication of B. abortus in AAMs, and for chronic infection, as inacti

168 overexpression of a protective antigen(s) of B. abortus in strain RB51 should enhance its vaccine eff

169 The galE sequence of B. abortus 2308 is more similar to galE from other anima

170 The complete genomic sequence of B. abortus provides an important resource for further in

171 A high prevalence of shedding of B. abortus (samples from India) and M. bovis (samples fr

172 fection rates in animals similar to those of B. abortus.

173 0, and virB11 are essential for virulence of B. abortus in mice, while functions encoded by the virB1

174 thought to be important to the virulence of B. abortus in pregnant ruminants.

175 h several fragments were shared between only B. abortus and B. suis, B. abortus shared more fragments

176 However, only B. abortus and B. abortus DNA induced high levels of IFN

177 Thus, opsonized B. abortus in human monocytes survives within phagosomes

178 n-12 p40 mRNA following either B. abortus or B. abortus-OVA administration.

179 xperimentally infected with B. melitensis or B. abortus and monitored for 24 weeks.

180 response to iron deprivation in the parental B. abortus 2308 strain, and a direct and specific intera

181 R. meliloti, and the intracellular pathogen B. abortus showed that this sequence conservation extend

182 e, LovhK (bab2_0652), functions as a primary B. abortus GSR sensor.

183 ile Ohr plays a prominent role in protecting B. abortus 2308 from organic hydroperoxide stress in in

184 hA-1 gene product participates in protecting B. abortus 2308 from oxidative damage.

185 imental findings indicate that SodC protects B. abortus 2308 from the respiratory burst of host macro

186 ntibody, suggesting that monocytes recognize B. abortus via their receptor for LPS.

187 hibitors of intracellular replication reduce B. abortus metabolism in axenic culture and perturb feat

188 conserved signalling pathway that regulates B. abortus stress physiology and infection biology.

189 nase functions as a photoreceptor regulating B. abortus virulence.

190 Rough B. abortus mutants were more sensitive to the bactericid

191 Strain RB51 was biotyped as a typical rough B. abortus biovar 1 (not strain 19) after animal passage

192 rmed upon three pathogenic Brucella species: B. abortus, B. melitensis, and B. suis.

193 w the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

194 d with those of the virulent parental strain B. abortus 2308.

195 triction in comparison to its parent strain, B. abortus 2308, when cultured in the presence of erythr

196 utant BEA5, and, unlike the parental strain, B. abortus BEA5 cannot utilize heme as an iron source in

197 and S708 phages that are lytic for B. suis, B. abortus and B. neotomae.

198 shared between only B. abortus and B. suis, B. abortus shared more fragments and had fewer nucleotid

199 Surprisingly, B. abortus induced down-regulation of CD28 and up-regula

200 We conclude that B. abortus stimulates a cellular as well as a humoral im

201 In the present study, we demonstrate that B. abortus and lipopolysaccharide (LPS) from B. abortus

202 t functional analyses that demonstrated that B. abortus RadA complements a radA defect in E. coli but

203 Together, these data indicate that B. abortus can directly activate human monocytes and pro

204 These findings indicate that B. abortus strain RB51 vaccination of mice induces speci

205 nsertions supports previous indications that B. abortus and B. melitensis share a common ancestor tha

206 We considered the possibility that B. abortus RadA might be compensating for the loss of Re

207 The results reveal that B. abortus causes a chronic infection of lung tissue in

208 These studies show that B. abortus strains that do not express catalase activity

209 resents a relevant environmental stress that B. abortus 2308 must deal with during its residence in t

210 The results of these studies suggest that B. abortus 2308 has at least one other heme oxygenase th

211 In conclusion, these data suggest that B. abortus can alter macrophage pathways to recruit addi

212 e B. abortus dhbCEBA operon, suggesting that B. abortus has the genetic capacity to produce a more co

213 nly for long-term persistence, suggests that B. abortus uses distinct sets of virulence determinants

214 The B. abortus bab1_1517 mutant strain was significantly att

215 The B. abortus bhuA mutant HR1703 exhibits significant atten

216 The B. abortus ftrA mutant exhibits significant attenuation

217 The B. abortus gal E mutant is not altered in colony morphol

218 The B. abortus GSR signaling pathway has multiple layers of

219 The B. abortus mntH mutant also exhibits extreme attenuation

220 The B. abortus mntH mutant MWV15 exhibits increased suscepti

221 The B. abortus sodC mutant was also found to be much more se

222 The B. abortus xthA-1 mutant also displayed reduced resistan

223 The B. abortus-induced IL-12 also enhanced NK cytolytic acti

224 Although the B. abortus xthA-1 mutant exhibited increased sensitivity

225 ved from these two Brucella species, and the B. abortus vaccine strain RB51 was assayed using normal

226 irulence in C57BL/6 and BALB/c mice, but the B. abortus ahpC katE double mutant is extremely attenuat

227 ntribute to the attenuation displayed by the B. abortus hfq mutant Hfq3 in the mouse model.

228 The attenuation displayed by the B. abortus sodC mutant in both resting and IFN-gamma-act

229 ichia coli mutants were used to identify the B. abortus genes (designated dhbC, -B, and -A) responsib

230 B. suis or B. melitensis were present in the B. abortus genome.

231 s was not the only mechanism involved in the B. abortus-mediated inhibition of the Th2 response to OV

232 Introduction of a bhuQ mutation into the B. abortus dhbC mutant BHB2 (which cannot produce sidero

233 killing by cultured murine macrophages, the B. abortus sodC mutant also displayed significant attenu

234 sible explanation for the attenuation of the B. abortus 2,3-DHBA-deficient mutant BHB1 in pregnant ru

235 parison of the growth characteristics of the B. abortus bhuA mutant and 2308 in this medium suggested

236 defect, suggesting that the inability of the B. abortus dhbC mutant to display wild-type growth in th

237 on analysis showed that transcription of the B. abortus dhbCEBA operon originates from two iron-regul

238 in-frame deletion of 660 bp with galE of the B. abortus genome by marker exchange.

239 A genome-scale analysis of the B. abortus GSR regulon identified stress response genes

240 The inability of the B. abortus hfq mutant to survive and replicate in a wild

241 in E. coli but could not act in place of the B. abortus RecA.

242 All of the B. abortus rough mutants exhibited a 4- to 5-log-unit in

243 rain 2308, and unlike its parent strain, the B. abortus bhuA mutant is unable to maintain a chronic s

244 Compared with the parental strain, the B. abortus ftrA mutant displays a decreased capacity to

245 d in mice spleens for a longer time than the B. abortus vaccine strain RB51, but as expected, neither

246 We conclude that the B. abortus GSR system regulates acute stress adaptation

247 Thus, the B. abortus GSR system is dispensable for colonization bu

248 nscription product of this gene binds to the B. abortus catalase promoter region.

249 Restoring 2,3-DHBA production to the B. abortus dhbC mutant by genetic complementation abolis

250 flanking regions immediately adjacent to the B. abortus dhbCEBA operon, suggesting that B. abortus ha

251 mental infection of pregnant cattle with the B. abortus dhbC mutant BHB1 clearly showed that producti

252 Mice infected with the B. abortus virB mutant elicited smaller increases in ser

253 the spleen during a mixed infection with the B. abortus wild type.

254 This B. abortus gene has been named wboA.

255 However, with time, B. abortus infection induced Beclin-1 cleavage with conc

256 her human pathogenic Brucella species and to B. abortus field isolate 9-941.

257 Conjugation to B. abortus was required for in vivo priming, since there

258 In contrast to B. abortus 2308, the isogenic hfq and bacA mutants remai

259 ins both B- and cytotoxic T-cell epitopes to B. abortus (B. abortus-MN 18-mer).

260 l diagnostic tool for monitoring exposure to B. abortus.

261 AIM2, and AIM2 KO mice are less resistant to B. abortus infection.

262 wnmodulate the Th1-like cytokine response to B. abortus and B. abortus DNA, which was confirmed by us

263 a coli LPS and DNA mirrored the responses to B. abortus components, suggesting that immune effects ob

264 ase-1 knockout mice were more susceptible to B. abortus infection than were wild-type animals, sugges

265 vaccine, since protection against wild-type B. abortus 2308 challenge was as effective as that obtai

266 ges in vitro increased recovery of wild-type B. abortus but not recovery of a virB mutant.

267 were higher in mice infected with wild-type B. abortus than in mice infected with the virB mutant, a

268 une response in mice infected with wild-type B. abortus than in mice infected with the virB mutant.

269 t in spleens of mice infected with wild-type B. abortus than in virB mutant-infected mice.

270 oad-host-range plasmid bearing the wild-type B. abortus wboA gene resulted in the restoration of O-si

271 -12p40 than did mice infected with wild-type B. abortus.

272 Unlike B. abortus, both smooth and rough strains of B. melitens

273 B. neotomae is a rodent pathogen, and unlike B. abortus, B. melitensis, and B. suis, B. neotomae has

274 cloned DHBA biosynthesis locus from virulent B. abortus 2308 and genetic complementation of defined E

275 gene encoding the 14-kDa protein in virulent B. abortus strain 2308 induced a rough-like phenotype wi

276 ndosomal pathway and replication of virulent B. abortus 2308 within these vesicles corresponds with a

277 ruption of the wboA gene in smooth, virulent B. abortus, Brucella melitensis, and Brucella suis resul

278 Immunity to a challenge with virulent B. abortus S2308 was not observed in the BaGroEL vaccina

279 f protection against challenge with virulent B. abortus strain 2308 or B. suis strain 1330 but no pro

280 e there was no induction of memory CTLs when B. abortus was only mixed with peptide.

281 ated, murine spleen cells were cultured with B. abortus derived DNA, lipopolysaccharide (LPS), or who

282 abortus 19 and later challenge exposed with B. abortus 2308.

283 ith Brucella abortus Infection of HBMEC with B. abortus induced the secretion of IL-6, IL-8, and MCP-

284 on-T and T cells following immunization with B. abortus.

285 bserved in the spleens of mice infected with B. abortus 2308 or a virB mutant.

286 Mice infected with B. abortus 2308 produced an antibody response to the pro

287 lls (astrocytes and microglia) infected with B. abortus also induced activation of HBMEC, but to a gr

288 Livers from mice infected with B. abortus displayed a fibrogenic phenotype with patches

289 e supernatant from macrophages infected with B. abortus induced bone marrow-derived monocytes (BMMs)

290 Yet, if LX-2 cells are infected with B. abortus, the capacity of supernatants from B. abortus

291 s from lemmings experimentally infected with B. abortus.

292 protein indicated that mice inoculated with B. abortus 19 or 2308 or B. melitensis RM1 also produced

293 Furthermore, macrophages preinfected with B. abortus S2308 or pretreated with B. abortus O polysac

294 ted with B. abortus S2308 or pretreated with B. abortus O polysaccharide did not prevent rough CA180-

295 ta suggest that the prior sensitization with B. abortus may induce a down regulation of the Th2 respo

296 cold-fixation complement fixation tests with B. abortus and B. melitensis antigens (CFA and CFM), USD

297 estern blots, sera from mice vaccinated with B. abortus RB51 recognized YajC but not SecD.

298 otein, splenocytes from mice vaccinated with B. abortus RB51 were able to proliferate and produce gam

299 antibodies from BALB/c mice vaccinated with B. abortus RB51.

300 screening of individual cows vaccinated with B. abortus S19 revealed no correlation between the immun