ライフサイエンスコーパス: B. bovis

1 B. bovis stimulates inducible nitric oxide synthase (iNO

2 B. bovis, an intraerythrocytic protozoal parasite, estab

3 B. bovis-specific T-helper lymphocyte culture supernatan

4 ase treatment of B. bovis extracts abrogated B. bovis-induced proliferation of peripheral blood monon

5 1), which confers partial protection against B. bovis challenge, is recognized by antibodies and T ly

6 recombinant RAP-1 protein responded against B. bovis, but not B. bigemina, merozoites.

7 mmatory mediators by E. coli, T. brucei, and B. bovis DNA was dependent on the presence of unmethylat

8 esia species, (B. bigemina, B. divergens and B. bovis).

9 Cocultures of Mphi and B. bovis-infected erythrocytes either in contact or phys

10 tion was used to explore the ability of anti-B. bovis Ig to interfere with IRBC cytoadhesion, and to

11 Argentina B. bovis strains R1A and S2P have msa-1 genes with amino

12 hat tick passage of the partially attenuated B. bovis T2Bo derivative strain further decreased virule

13 h attenuation, but when retained, attenuated B. bovis can revert to virulence following tick passage.

14 2 locus was characterized from 12 Australian B. bovis strains and isolates, including two vaccine str

15 udy, sequencing of MSA-1 from two Australian B. bovis vaccine strains and 14 breakthrough isolates fr

16 port that monocyte-derived Mphi activated by B. bovis expressed enhanced levels of inflammatory cytok

17 the antiparasitic activity of NO produced by B. bovis-stimulated Mphi has not been definitively demon

18 isms that were mitogenic for murine B cells, B. bovis DNA is largely nonmethylated and induced a dose

19 ites is completely conserved among different B. bovis strains.

20 rved among otherwise antigenically different B. bovis strains.

21 Th-cell epitopes are conserved in different B. bovis strains but not in B. bigemina RAP-1.

22 ynthase mRNA in bovine macrophages by either B. bovis-parasitized erythrocytes and IFN-gamma or CM wa

23 Both PCR-based and CF tests for B. bovis had high specificity values ranging from 96 to

24 nsitivities of the three PCR-based tests for B. bovis ranged from 58 to 70% for a single determinatio

25 e in vitro effect of intact and fractionated B. bovis merozoites on bovine macrophage nitric oxide (N

26 gly immunogenic for T helper (Th) cells from B. bovis-immune cattle and that like B-cell epitopes, Th

27 for stimulation of T-cell lines derived from B. bovis-immune cattle.

28 Furthermore, B. bovis and E. coli DNAs enhanced immunoglobulin secret

29 In the presence of IFN-gamma, B. bovis merozoites stimulated NO production, as indicat

30 order E. coli > or = T. cruzi > T. brucei > B. bovis.

31 acted predominantly with spherical bodies in B. bovis merozoites.

32 een antigenic variation and cytoadherence in B. bovis and suggest that the VESA1 Ag acts as an endoth

33 ro model to study the components involved in B. bovis cytoadherence and sequestration.

34 The 60-kDa B. bovis RAP-1 is recognized by antibodies and T lymphoc

35 rotection from disease, an in vitro assay of B. bovis sequestration was used to explore the ability o

36 We recently reported that the NT domain of B. bovis RAP-1 contained immunodominant T-cell epitopes,

37 This study evaluated the efficiency of B. bovis infection within Rhipicephalus (Boophilus) micr

38 Furthermore, a lipid fraction of B. bovis-infected erythrocytes stimulated iNOS expressio

39 rine and human B cells, an 11-kb fragment of B. bovis DNA was analyzed for CG dinucleotide content an

40 chemical donors of NO inhibit the growth of B. bovis in vitro.

41 in surface-specific immunoprecipitations of B. bovis-IRBCs.

42 onal factors contribute to the inhibition of B. bovis replication.

43 The results indicate that isolates of B. bovis capable of evading vaccine-induced immunity con

44 the biologically cloned Mexico Mo7 strain of B. bovis and identified the sequence differences between

45 ttle hyperinfected with the Mexico strain of B. bovis and shown to be clinically immune did not cross

46 mmune to challenge with the Mexico strain of B. bovis proliferated against recombinant B. bovis RAP-1

47 SA-2 proteins in the Argentina R1A strain of B. bovis with the Mexico Mo7 clone revealed a relatively

48 le inoculum of a cloned laboratory strain of B. bovis.

49 riant virulent tick-transmissible strains of B. bovis and that R. microplus ticks could acquire and t

50 ico, Texas, Australia, and Israel strains of B. bovis but neither B. bigemina merozoites nor recombin

51 otherwise antigenically different strains of B. bovis, supports the inclusion of this region in vacci

52 cribed babr 0.8 gene in Australia strains of B. bovis.

53 DNase treatment of B. bovis extracts abrogated B. bovis-induced proliferati

54 ant erythrocyte surface antigen 1 (VESA1) of B. bovis IRBCs.

55 erythrocytes but not on either uninfected or B. bovis-parasitized erythrocytes.

56 mine whether DNA from the protozan parasites B. bovis, Trypanosoma cruzi, and T. brucei activates mac

57 of B. bovis proliferated against recombinant B. bovis RAP-1 protein derived from the Mexico strain.

58 Sequencing analysis of select B. bovis genes before and after tick passage showed sign

59 sults indicate the potential to use selected B. bovis RAP-1 peptides as immunogens to prime for stron

60 dy the components involved in sequestration, B. bovis parasites that induce adhesion of the infected

61 t B. bovis merozoites and antigen-stimulated B. bovis-immune T cells can induce the production of NO,

62 These findings demonstrate that B. bovis induces an innate immune response that is capab

63 The studies reported here demonstrate that B. bovis RAP-1 is strongly immunogenic for T helper (Th)

64 These results show that B. bovis merozoites and antigen-stimulated B. bovis-immu

65 It was previously shown that B. bovis RAP-1 associates with the merozoite surface as

66 Ag acts as an endothelial cell ligand on the B. bovis-IRBC.

67 ns using sera from animals infected with the B. bovis vaccine strains.

68 CD4(+)-T-cell lines from three B. bovis-immune cattle with different DRB3 haplotypes re

69 ive type 1 immune responses upon exposure to B. bovis.

70 tory for T lymphocytes from cattle immune to B. bovis.

71 The lack of response to B. bovis RAP-1 indicated that a strictly conserved 14-am

72 r, NO produced by bovine Mphi in response to B. bovis-infected erythrocytes was only partially respon

73 nfection rates of 22% to 30% and transmitted B. bovis during transmission feeding.

74 ufficient to protect calves against virulent B. bovis challenge.

75 t both calves and ticks can support virulent B. bovis coinfection through all phases of the hemoparas

76 by repeated stimulation of lymphocytes with B. bovis membrane antigen proliferated strongly against