ライフサイエンスコーパス: B. cenocepacia

1 B. cenocepacia (genomovar III) is the most prevalent and

2 B. cenocepacia can survive intracellularly in macrophage

3 B. cenocepacia efficiently activates the inflammasome an

4 B. cenocepacia employs a type VI secretion system (T6SS)

5 B. cenocepacia strain J2315 was isolated from a CF patie

6 B. cenocepacia zmpB and zmpA zmpB mutants had no proteol

7 ed the efficacy of phage therapy in an acute B. cenocepacia lung infection model.

8 Although B. cenocepacia strains can be isolated from soil and can

9 nically relevant species, B. multivorans and B. cenocepacia.

10 to these responses, the interaction between B. cenocepacia and Toll-like receptor 5 (TLR5) was inves

11 demonstrate that production of ornibactin by B. cenocepacia in response to iron starvation requires t

12 cies as zmpA and was detected in B. cepacia, B. cenocepacia, B. stabilis, B. ambifaria, and B. pyrroc

13 Within the Burkholderia cepacia complex, B. cenocepacia is the most common species associated wit

14 ile they share similar genetic compositions, B. cenocepacia and B. multivorans exhibit important diff

15 ectron acceptors under anaerobic conditions, B. cenocepacia and B. multivorans used fermentation rath

16 BCP-deficient B. cenocepacia exhibit a growth-phase-dependent hypersen

17 receptor 5 (TLR5) contributes to exacerbate B. cenocepacia-induced lung epithelial inflammatory resp

18 t that P. aeruginosa alginate may facilitate B. cenocepacia infection by interfering with host innate

19 e whether P. aeruginosa alginate facilitates B. cenocepacia infection in mice, cystic fibrosis transm

20 their ability to secrete IL-1beta following B. cenocepacia infection, suggesting that a deficiency i

21 vity of TecA is necessary and sufficient for B. cenocepacia-triggered lung inflammation and also prot

22 pid A explaining the ability of hypoacylated B. cenocepacia LPS to promote proinflammatory responses

23 pid A, suggesting lipid A penta-acylation in B. cenocepacia is required not only for bacterial growth

24 nto the control of cable pilus biogenesis in B. cenocepacia and provide evidence for regulation of cb

25 lagellin subunit, fliCII, was constructed in B. cenocepacia K56-2 and tested in a murine agar bead mo

26 dy highlights strain specific differences in B. cenocepacia virulence mechanisms important for unders

27 ression of cable pilus biosynthetic genes in B. cenocepacia.

28 n model, indicating that zmpB is involved in B. cenocepacia virulence.

29 he role of the PI3K/Akt signaling pathway in B. cenocepacia-infected monocytes and macrophages.

30 s a natural substrate for the efflux pump in B. cenocepacia and imply that the environment of low iro

31 our highly conserved periplasmic residues in B. cenocepacia ArnT, tyrosine-43, lysine-69, arginine-25

32 identified functional pathways that indicate B. cenocepacia can produce a wider array of virulence fa

33 an pyrin is required to detect intracellular B. cenocepacia leading to IL-1beta processing and releas

34 We also show that intracellular B. cenocepacia within macrophages produced more penta-ac

35 growth capabilities of B. cenocepacia J2315, B. cenocepacia K56-2, and B. multivorans ATCC 17616 on 1

36 lammation and also protects mice from lethal B. cenocepacia infection.

37 ole of Cbl pili and the adhesin in mediating B. cenocepacia binding to and transmigration across squa

38 In contrast to the native B. cenocepacia enzyme, thioredoxin is the preferred redo

39 tate (3-OH C14:0) relative to the lipid A of B. cenocepacia LMG 12614.

40 rst comprehensive genome-phenome analyses of B. cenocepacia infection in cystic fibrosis lungs and se

41 edictions to in vitro growth capabilities of B. cenocepacia J2315, B. cenocepacia K56-2, and B. multi

42 g and transmigration or invasion capacity of B. cenocepacia.

43 To understand the contribution of B. cenocepacia flagella to infection, a strain mutated i

44 Ingestion of B. cenocepacia independently contributes to and worsens

45 seven lacZ fusions in a clinical isolate of B. cenocepacia that are inducible by octanoyl-HSL.

46 By immunoscreening an expression library of B. cenocepacia isolate BC7, we identified a large gene (

47 man, J2315 is representative of a lineage of B. cenocepacia rarely isolated from the environment and

48 ells infected with T6SS-defective mutants of B. cenocepacia, suggesting that the inflammatory reactio

49 Further, alginate decreased phagocytosis of B. cenocepacia by professional phagocytes both in vivo a

50 k confirmed the proinflammatory potential of B. cenocepacia penta-acylated lipid A.

51 ts show that the intracellular processing of B. cenocepacia is similar in both professional and nonpr

52 lar survival, replication, and processing of B. cenocepacia.

53 ing to CK13 and transmigration properties of B. cenocepacia.

54 e-scale metabolic network reconstructions of B. cenocepacia J2315 and B. multivorans ATCC 17616 in pa

55 ntributes to the survival and replication of B. cenocepacia in eukaryotic cells.

56 ss differential expression, protein spots of B. cenocepacia and B. multivorans that were unique or di

57 Certain strains of B. cenocepacia express peritrichous adherence organelles

58 is unique in comparison to other strains of B. cenocepacia, highlighting the genomic plasticity of t

59 We examined the transmigration of B. cenocepacia through polarized respiratory epithelium.

60 fied as being required for full virulence of B. cenocepacia K56-2.

61 million bases of cDNA from 2 closely related B. cenocepacia strains (one isolated from a CF patient a

62 remain confined to the endobronchial spaces, B. cenocepacia can traverse airway epithelium to cause b

63 We purified LPS from two strains, B. cenocepacia LMG 12614 and B. multivorans LMG 14273, e

64 Together, these results demonstrate that B. cenocepacia flagella contribute to virulence in an in

65 In this study, we determined that B. cenocepacia has an additional metalloprotease, which

66 Recent studies indicate that B. cenocepacia survives within macrophages and airway ep

67 which supports the clinical observation that B. cenocepacia is more virulent than B. multivorans.

68 In this study, we report that B. cenocepacia flagellin is glycosylated on at least 10

69 Here, we report that B. cenocepacia has only one late acyltransferase, LpxL (

70 Here, we report that B. cenocepacia LPS strongly activates human TLR4.MD-2 de

71 These observations suggest that B. cenocepacia traverses polarized respiratory epitheliu

72 The B. cenocepacia cblBACDS operon encodes the structural an

73 hains and the aminoarabinose residues in the B. cenocepacia lipid A allow exposure of the fifth acyl

74 We demonstrate that the B. cenocepacia BCP (BcBCP) homologue functions through a

75 Our results demonstrate that the B. cenocepacia cblS, cblT, and cblR genes are essential

76 nome of the previously described therapeutic B. cenocepacia podophage BcepIL02 and its close relative

77 esponses of human airway epithelial cells to B. cenocepacia infection.

78 epithelial cells and alveolar macrophages to B. cenocepacia infection.

79 tivation and IL-1beta release in response to B. cenocepacia challenge.

80 or ASC induced a robust IL-1beta response to B. cenocepacia, which correlated with enhanced host cell

81 to airway infections, yet their responses to B. cenocepacia have not been fully investigated.

82 pression of either cblS or cblR in wild-type B. cenocepacia strain BC7 led to a significant increase,

83 for controlling excessive inflammation upon B. cenocepacia infection.

84 activity within and between genomovars, with B. cenocepacia strains possessing the greatest cytokine

85 P-pyrin and ASC (YFP-ASC) were infected with B. cenocepacia and analyzed for inflammasome activation.

86 e regulator knockout mice were infected with B. cenocepacia strain BC7 suspended in either phosphate-

87 hat in human mononuclear cells infected with B. cenocepacia, pyrin associates with caspase-1 and ASC

88 inflammatory cytokines during infection with B. cenocepacia.

89 pathway during infection of macrophages with B. cenocepacia.