ライフサイエンスコーパス: B. cereus

1 B. cereus and B. thuringiensis sigP and rsiP homologues

2 B. cereus and B. thuringiensis, species closely related

3 B. cereus endophthalmitis followed a more rapid and viru

4 B. cereus G9241 also harbors S-layer genes, including ho

5 B. cereus G9241 encodes two megaplasmids, pBCXO1 and pBC

6 B. cereus G9241 was avirulent in New Zealand rabbits aft

7 B. cereus induced the leakage of albumin and fibrin into

8 B. cereus infection of polarized RPE cell monolayers res

9 B. cereus replicated more rapidly in the eyes of TNFalph

10 B. cereus strain G9241 expresses anthrax toxin, several

11 Kurstaki HD-1, B. cereus T, and the nonpathogenic strain B. anthracis S

12 Three isolates, Bacillus cereus D-17, B. cereus 43881, and Bacillus thuringiensis 33679, conta

13 ing a comparative genome hybridization of 19 B. cereus and Bacillus thuringiensis strains against a B

14 nome sequencing of B. thuringiensis 97-27and B. cereus E33L was undertaken to identify shared and uni

15 Here we used a genetic approach to analyze B. cereus G9241 S-layer assembly and function.

16 ith the HF-PSs from B. cereus ATCC 10987 and B. cereus ATCC 14579 showed that the B. anthracis struct

17 npathogenic strains B. cereus ATCC 10987 and B. cereus ATCC 14579.

18 of pathogenic strains B. anthracis Ames and B. cereus G9241 and nonpathogenic strains B. cereus ATCC

19 llagen-like regions of both B. anthracis and B. cereus are similarly substituted by short O-glycans t

20 isms such as B. anthracis, C. botulinum, and B. cereus, which can to be used for method validation, i

21 drug resistance in both Escherichia coli and B. cereus.

22 x toxins as well as hyaluronic acid (HA) and B. cereus exopolysaccharide (BPS) capsules.

23 Kurstaki, and B. cereus T, respectively.

24 lated Bacillus species, B. licheniformis and B. cereus, indicating that the biofilm-promoting effect

25 ivity against strains of S. aureus, MRSA and B. cereus (MIC=187.5-365mug/ml).

26 thuringiensis, and B. atrophaeus spores, and B. cereus vegetative cells were investigated by Raman im

27 ormally occurs in Bacillus thuringiensis and B. cereus can be mimicked by tethering the peptide to Pl

28 including E. coli type IA topoisomerases and B. cereus topoisomerase I (bcTopo I) and IIIalpha (bcTop

29 (a FO analog) is bound by YxeB in vitro and B. cereus imports or binds Cr-DFO in vivo.

30 4, isolated from a necrotic human wound, and B. cereus E33L, which was isolated from a swab of a zebr

31 d on MLST, we selected several B. anthracis, B. cereus, and B. thuringiensis strains and compared the

32 bla1 is poorly transcribed in B. anthracis, B. cereus, and B. thuringiensis.

33 ene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates.

34 This is the tightest binding observed of any B. cereus siderophore-binding protein.

35 In contrast, exogenously applied B. cereus sphingomyelinase, despite causing higher eleva

36 nly one strain, which was later confirmed as B. cereus, gave a positive result.

37 16S rRNA analysis identified this isolate as B. cereus, the rapid generation and analysis of a high-c

38 dentify the genes that are conserved between B. cereus and B. anthracis, and the genes that are uniqu

39 differences in virulence regulation between B. cereus and B. anthracis.

40 omplete release of endogenous CaDPA for both B. cereus and B. subtilis spores; (3) the rate but not t

41 led large potential plasmids present in both B. cereus 43881 (341 kb) and B. thuringiensis ATCC 33679

42 tance determinant that is functional in both B. cereus and E. coli.

43 A case of bacteremia caused by B. cereus in a 19-day-old preterm neonate who was succes

44 reduce the occurrence of outbreaks caused by B. cereus, C. perfringens, and S. aureus in the United S

45 ve bacterium to both ZEO or MEO, followed by B. cereus and E. coli.

46 Hemolysis by B. cereus results largely from the action of phosphatidy

47 iofilm matrix-inducing compounds produced by B. cereus.

48 (QTL) associated with disease suppression by B. cereus explained 38% of the phenotypic variation amon

49 In two cases, QTL for disease suppression by B. cereus map to the same locations as QTL for other tra

50 ance to P. torulosum, disease suppression by B. cereus, and growth of B. cereus on the seed.

51 type, nonswarming, and swarming-complemented B. cereus strains grew to a similar number in the vitreo

52 ng growth under capsule-inducing conditions, B. cereus G9241 assembled BSLs (BslA and BslO) and the S

53 Here we fractionated crude B. cereus culture supernatant by anion-exchange chromato

54 d with wild-type or quorum-sensing-deficient B. cereus, and cytotoxicity was analyzed.

55 ay be used as a suitable biosensor to detect B. cereus and to become a portable system for food quali

56 or the sap gene from either of two different B. cereus strains that are sensitive to AP50c infection

57 directed genome sequencing of seven diverse B. cereus strains to identify novel sequences encoded in

58 hesized that intraocular inflammation during B. cereus endophthalmitis would be controlled by MyD88-

59 pathways, contributed to inflammation during B. cereus endophthalmitis.

60 Residual inflammation observed during B. cereus endophthalmitis in TLR2(-/-) mice led us to in

61 ng a multicopy plasmid containing the entire B. cereus plc-sph operon.

62 zation of two clinical and one environmental B. cereus isolate collected during an investigation of t

63 Experimental B. cereus endophthalmitis was induced in wild-type contr

64 ogen containment by PMNs during experimental B. cereus endophthalmitis.

65 h or without dexamethasone, for experimental B. cereus endophthalmitis.

66 flammatory response observed in experimental B. cereus endophthalmitis, identifying a novel innate im

67 curs during the early stages of experimental B. cereus endophthalmitis, beginning as early as 4 hours

68 e overall course or severity of experimental B. cereus endophthalmitis.

69 ted clones provided useful markers to follow B. cereus populations on plant surfaces.

70 han the minimal inhibitory concentration for B. cereus when measured at 8 hours.

71 nsis (BT) isolates, and one isolate each for B. cereus (BC), B. mycoides (BM), B. atrophaeus (BG), an

72 bly other roundworms can be common hosts for B. cereus-group bacteria, findings with important ecolog

73 ifying a novel innate immune interaction for B. cereus and for this disease.

74 plcR-regulated toxins were not required for B. cereus-induced RPE cytotoxicity, but these toxins did

75 However, the LD(50)s for B. cereus G9241 in both mouse strains were markedly high

76 We also analyzed 30 gyrB sequences for B. cereus group strains with published 16S rRNA sequence

77 of B. thuringiensis that sets it apart from B. cereus and B. anthracis is the production of crystal

78 thuringiensis are readily distinguished from B. cereus by the presence of plasmid-borne specific toxi

79 29000 clones containing chromosomal DNA from B. cereus strain UW85.

80 a corresponding fragment of genomic DNA from B. cereus UW85.

81 he three isolates was indistinguishable from B. cereus G9241.

82 structure of the metallo-beta-lactamase from B. cereus.

83 cin A, we constructed a genomic library from B. cereus UW85, which produces zwittermicin A, and scree

84 In this study the plasmids from B. cereus isolates that produce emetic toxin or are link

85 structural data obtained for the HF-PS from B. cereus type strain ATCC 14579 revealed that each HF-P

86 common structural feature in the HF-PSs from B. cereus ATCC 10987 and B. anthracis was the presence o

87 Comparison with the HF-PSs from B. cereus ATCC 10987 and B. cereus ATCC 14579 showed tha

88 atic characterization of PC-PLC and SPH from B. cereus and B. anthracis.

89 against a lethal challenge with spores from B. cereus G9241 or B. cereus Elc4, a strain that had bee

90 Deletion of the Vb beta-strand from B. cereus PI-PLC abolished its ability to cleave GPI-anc

91 ttermicin A self-resistance gene, zmaR, from B. cereus UW85 revealed three open reading frames (ORFs)

92 Furthermore, B. cereus G9241 spores could germinate and disseminate a

93 The spores of the Bacillus cereus group (B. cereus, Bacillus anthracis, and Bacillus thuringiensi

94 most common species of the B. cereus group, B. cereus, Bacillus thuringiensis, and Bacillus mycoides

95 pBC218 harbours bpsX-H, B. cereus exo-polysaccharide, which produce a second cap

96 lated to each other than to other identified B. cereus plasmids.

97 In B. cereus, PlcR was found to be a positive regulator of

98 In B. cereus, these genes are organized in an operon regula

99 ty and differences in functional activity in B. cereus RC607 and after cloning of the mer determinant

100 an important role for these TLR adaptors in B. cereus endophthalmitis.

101 glucosaminidase function that is apparent in B. cereus/B. anthracis.

102 ted assembly of S-layer proteins and BSLs in B. cereus G9241 contributes to the pathogenesis of anthr

103 microbial therapies; its characterization in B. cereus is described here.

104 The bla2 gene is strongly expressed in B. cereus and B. thuringiensis and weakly expressed in B

105 8 clones) was screened for GFP expression in B. cereus UW85 using a 96-well microtiter dish assay.

106 itive mutants and that zmaR is functional in B. cereus.

107 These enzymes and genes in B. cereus are nearly identical to those in the very clos

108 Here, we show two genes in B. cereus ATCC 14579 encoding enzymes involved in the sy

109 and rice dishes were commonly implicated in B. cereus outbreaks (50%).

110 . subtilis spores and > or =20-fold lower in B. cereus and B. megaterium spores.

111 oreover, we identified a four-gene operon in B. cereus ATCC 14579 that encodes proteins with the foll

112 psule-encoding plasmids pBCXO1 and pBC218 in B. cereus G9241 alone is insufficient to render the stra

113 Vomiting was commonly reported in B. cereus (median, 75% of cases) and S. aureus outbreaks

114 This Vb beta-strand in B. cereus PI-PLC forms contacts with the glycan linker o

115 es, indicating an important role for TLR4 in B. cereus endophthalmitis.

116 suggested to be associated with virulence in B. cereus and B. anthracis, respectively.

117 s, or S. aureus, (ii) metabolically inactive B. cereus, E. faecalis, or S. aureus, (iii) sacculus pre

118 actors within the B. cereus group, including B. cereus, B. anthracis, and B. thuringiensis.

119 several human and plant pathogens, including B. cereus, B. anthracis, and B. thuringiensis.

120 lease (T(release)) of hundreds of individual B. cereus spores germinating with both saturating and su

121 During infection, B. cereus G9241 elaborates both hasACB and bpsX-H capsul

122 In one such infection, B. cereus G9241 was identified as the causative agent of

123 including Bacillus anthracis (11 isolates), B. cereus (38 isolates), Bacillus mycoides (1 isolate),

124 Lastly, B. cereus G9241 derivatives cured of one or both megapla

125 Although most B. cereus isolates are not opportunistic pathogens and o

126 Wild-type and mutant B. cereus sterile supernatants induced blood-ocular barr

127 A quorum-sensing mutant B. cereus strain caused BRB permeability comparable to t

128 the results of detailed study of three novel B. cereus phages, two highly related myoviruses (JL and

129 e 1 comprised B. anthracis strains, numerous B. cereus strains, and rare B. thuringiensis strains, wh

130 ymorphism within a collection of over 300 of B. cereus, B. thuringiensis, and B. anthracis isolates,

131 The beta-galactosidase activities of B. cereus group species harboring bla promoter-lacZ tran

132 Northern blot analysis of B. cereus RNA showed a 5.5-kb transcript which hybridize

133 e apparent among the different categories of B. cereus (isolates from food poisoning incidents and no

134 ts with severe pneumonia, in a collection of B. cereus isolates associated with human illness.

135 he biosensor sensitivity in pure cultures of B. cereus was found to be 10(0) colony forming units per

136 Detection of B. cereus was carried out based on an increase in the ch

137 of this study was to analyze the effects of B. cereus infection and plcR-regulated toxins on the bar

138 sults demonstrate the deleterious effects of B. cereus infection on RPE barrier function and suggest

139 High level expression of B. cereus BioC in E. coli blocked cell growth and fatty

140 ine the extent of heterologous expression of B. cereus genes in the library, we screened it for expre

141 ExsM, from a beta-mercaptoethanol extract of B. cereus ATCC 4342 spores.

142 cting relatedness among microbial genomes of B. cereus group members and potentially may circumvent t

143 ease suppression by B. cereus, and growth of B. cereus on the seed.

144 Upon stable transfection and induction of B. cereus sphingomyelinase, there were increases in neut

145 Injection of B. cereus or S. aureus culture fluids caused both signif

146 ) CFU of either a clinical ocular isolate of B. cereus producing hemolysin BL (HBL+) or an isogenic m

147 uence homogeneity, environmental isolates of B. cereus and B. thuringiensis exhibit extensive genetic

148 erminal end of the type II beta-lactamase of B. cereus.

149 ral protein of the exosporium basal layer of B. cereus family spores and that it can self-assemble in

150 Migration of B. cereus throughout the eye during endophthalmitis is a

151 Expression by L. monocytogenes of B. cereus PI-PLC, which has strong activity on GPI-ancho

152 ng fragments were not detected in mutants of B. cereus UW85 that were sensitive to zwittermicin A, an

153 floxacin improved the therapeutic outcome of B. cereus endophthalmitis.

154 As the pathogenesis of B. cereus anthrax-like disease in mice is dependent on p

155 toxin, hemolysin BL, to the pathogenesis of B. cereus infection in an endophthalmitis system that is

156 walls all contribute to the pathogenesis of B. cereus, S. aureus, and E. faecalis endophthalmitis in

157 hrax may be important in the pathogenesis of B. cereus.

158 their contributions to the pathogenicity of B. cereus group bacteria.

159 Screening of a population of B. cereus group isolates revealed that pXO1-like plasmid

160 The presence of B. cereus strains in six of the seven subgroups and the

161 findings suggest a new mode of regulation of B. cereus virulence and reveal intriguing similarities a

162 nce typing (MLST) refined the relatedness of B. cereus group members by separating them into clades a

163 The complete genome sequence of B. cereus ATCC 14579 together with the gapped genome of

164 ecifically detect the target DNA sequence of B. cereus from other bacteria that can be found in dairy

165 analysis based on whole-genome sequences of B. cereus sensu lato strains revealed several closely re

166 as well as aerosol challenge with spores of B. cereus G9241, harboring pBCXO1 and pBC218 virulence p

167 Three recently identified clade 1 strains of B. cereus that caused severe pneumonia, i.e., strains 03

168 th 33 strains of B. anthracis, 27 strains of B. cereus, and 9 strains of B. thuringiensis.

169 warming, or swarming-complemented strains of B. cereus.

170 Four high resolution crystal structures of B. cereus PPM revealed the active site architecture, ide

171 ovide virulence factors, making the study of B. cereus phages important to understanding the evolutio

172 7 HF-PS structure was different from that of B. cereus ATCC 14579.

173 s do not support the proposed unification of B. cereus and B. thuringiensis into one species.

174 ly injected with 100 colony-forming units of B. cereus, and eyes were analyzed at specific times afte

175 ravitreally with 100 colony-forming units of B. cereus.

176 The virulence of B. cereus endophthalmitis historically has been attribut

177 Here we evaluated the virulence of B. cereus G9241 as well as the contributions of pBCXO1 a

178 owever, like B. anthracis, full virulence of B. cereus G9241 for mice requires the presence of both p

179 he formation of cereose-containing glycan on B. cereus spores.

180 four other phage loci in B. anthracis and/or B. cereus lysogens.

181 hallenge with spores from B. cereus G9241 or B. cereus Elc4, a strain that had been isolated from a f

182 rulence plasmids have been acquired by other B. cereus strains and enable the pathogenesis of anthrax

183 the pXO1-like plasmids may define pathogenic B. cereus isolates in the same way that pXO1 and pXO2 de

184 B. anthracis and other pathogenic B. cereus isolates harbor genes for the secretion of S-l

185 ato strains revealed several closely related B. cereus and B. thuringiensis strains that carry sap ge

186 ltures, respectively, of its close relatives B. cereus, B. thuringiensis, and B. mycoides derived fro

187 re than 150 nucleotide differences separated B. cereus and B. mycoides from B. anthracis in pairwise

188 metry and cell sorting efficiently separated B. cereus cells expressing GFP from a 10000-fold excess

189 -M) system genes is presented from sequenced B. cereus, Bacillus anthracis, and Bacillus thuringiensi

190 itutions in the chromosome; however, several B. cereus genomes isolated from soil and not previously

191 ry, we screened it for expression of several B. cereus activities in the E. coli host.

192 or the other species, although just a single B. cereus strain was fully resistant.

193 resistant to tetracycline and a further six B. cereus and one B. thuringiensis cultures fell into th

194 pecific to B. anthracis was detected in some B. cereus strains.

195 lnesses, these results demonstrate that some B. cereus strains can cause severe and even fatal infect

196 B. thuringiensis strains together with some B. cereus strains.

197 nes genetically to identify rapidly specific B. cereus loci.

198 e sequencing and analysis of the type strain B. cereus ATCC 14579.

199 a rare susceptible non-B. anthracis strain, B. cereus ATCC 4342.

200 nd B. cereus G9241 and nonpathogenic strains B. cereus ATCC 10987 and B. cereus ATCC 14579.

201 ntrast, the cell walls from clade 2 strains (B. cereus type strain ATCC 14579 and B. thuringiensis st

202 Surprisingly, B. cereus-infected TLR4(-/-) eyes also had significantly

203 We have demonstrated that B. cereus ATCC 14579 takes up (55)Fe radiolabeled ferric

204 We also determined that B. cereus produces additional retinal toxins that might

205 We report here that B. cereus G9241 causes anthrax-like disease in immune-co

206 These cases are interesting in that B. cereus, even from blood or sputum specimens, may ofte

207 cters (polymorphic fragments) indicates that B. cereus and B. thuringiensis are the closest taxa to B

208 ysis of bacterial localization revealed that B. cereus uniquely migrated rapidly from posterior to an

209 growth assays using DFO and Cr-DFO show that B. cereus selectively imports and uses FO when DFO is pr

210 We suggest that B. cereus and its close relatives, easily isolated from

211 The B. cereus clade 1 strains had cell walls that were simil

212 The B. cereus E33L isolate appears to be the nearest relativ

213 The B. cereus G9241 csaB mutant assembled capsular polysacch

214 ected in B. anthracis strains containing the B. cereus plcR gene on a multicopy plasmid under control

215 The core genome size for the B. cereus s.l. group was approximately 1750 genes, with

216 illus cereus ATCC 10987 strain, and from the B. cereus ATCC 14579 type strain and compared with those

217 ow that a related endolysin (Ply21) from the B. cereus phage, TP21, shows a similar pattern of behavi

218 The protein sequence inferred from the B. cereus phosphonatase gene was determined, and this se

219 l other protein biomarkers isolated from the B. cereus T spores.

220 chiff base mechanism known to operate in the B. cereus enzyme was verified for the S. typhimurium enz

221 nc content to mimic that which occurs in the B. cereus enzyme.

222 ndidates that most likely play a role in the B. cereus group pathogenicity.

223 . anthracis from other microorganisms in the B. cereus group.

224 74 23S rRNA sequences for all species in the B. cereus group.

225 Transcription of the B. cereus and B. thuringiensis CDC genes is controlled b

226 Four of the B. cereus and one of the B. thuringiensis cultures were

227 t found in the di- and monozinc forms of the B. cereus enzyme (12 and 6 s(-)(1), respectively).

228 s library provides 5.75-fold coverage of the B. cereus genome, with an average insert size of 98 kb.

229 This classification of the B. cereus group conflicts with current taxonomic groupin

230 , which is a biomarker characteristic of the B. cereus group of bacteria, was determined from a fragm

231 an abundant outer spore coat protein of the B. cereus group with a prominent role in spore resistanc

232 ed that the three most common species of the B. cereus group, B. cereus, Bacillus thuringiensis, and

233 nalysis of the genomes of two members of the B. cereus group, B. thuringiensis 97-27 subsp. konkukian

234 siderophore produced by many members of the B. cereus group, including B. anthracis.

235 Structural and functional examination of the B. cereus metA protein reveals that a single amino acid

236 Surprisingly, functional analysis of the B. cereus metA protein shows that it does not use succin

237 model for the kinetically active form of the B. cereus metalloenzyme is proposed.

238 Expression of the B. cereus or B. thuringiensis sigP and rsiP genes in a B

239 Replacement of the B. cereus plcR gene by its truncated orthologue from B.

240 hereas introduction into B. anthracis of the B. cereus plcR gene with its own promoter did not activa

241 Some members of the B. cereus sensu lato group produce CDP-3-C-methyl-6-deox

242 gram-positive, spore-forming bacteria of the B. cereus sensu lato group.

243 w insights to the successful survival of the B. cereus species in natural environments or in the host

244 The results showed that the B. cereus ATCC 10987 HF-PS has a repeating oligosacchari

245 h of these HF-PSs and, furthermore, that the B. cereus ATCC 10987 HF-PS structure was different from

246 is of site-directed mutants reveals that the B. cereus metA protein and the E. coli HTS share a commo

247 Therefore, the B. cereus metA protein functions as an HTA despite great

248 to encode a protein with 92% identity to the B. cereus type II enzyme.

249 he irreversible inhibition observed with the B. cereus ADI from the time controlled inhibition observ

250 ce variations, the microorganisms within the B. cereus group were divided into seven subgroups, Anthr

251 genetic transfer of such factors within the B. cereus group, including B. cereus, B. anthracis, and

252 specificity of BclA glycosylation within the B. cereus group.

253 Comparative sequence analyses of these B. cereus plasmids revealed a high degree of sequence si

254 Even though B. cereus and B. thuringiensis contain the ppk and ppx g

255 ports our conclusion that HBL contributes to B. cereus virulence and implicates PC-PLC and collagenas

256 g binding of the cell wall-binding domain to B. cereus but not to other species tested.

257 ly interleukin-6 was produced in response to B. cereus infection.

258 C57BL/6J mice were comparably susceptible to B. cereus G9241 by both subcutaneous and intranasal rout

259 Compared to wild-type B. cereus G9241, spores with a deletion of the pBCXO1-ca

260 ally with approximately 100 CFU of wild-type B. cereus or B. thuringiensis or a plcR-deficient mutant

261 permeability comparable to that of wild-type B. cereus.

262 were injected intravitreally with (i) viable B. cereus, E. faecalis, or S. aureus, (ii) metabolically

263 ed identifications are largely due to a weak B. cereus signal in the bacterial mixtures.

264 roversial results, and it is unclear whether B. cereus, B. anthracis and B. thuringiensis are varieti

265 A/J mice challenged with B. cereus G9241 confirmed the virulence of this strain.