ライフサイエンスコーパス: B. fragilis

1 B. fragilis 9343 expresses at least three capsular polys

2 B. fragilis enterotoxin did not affect enterocyte viabil

3 B. fragilis enterotoxin was associated with HT-29 cell r

4 B. fragilis lacking PSA is unable to restrain T helper 1

5 B. fragilis OxyR and Dps proteins showed high identity t

6 B. fragilis was anergistic (antagonistic) to E. coli in

7 B. fragilis, as well as purified capsular polysaccharide

8 B. fragilis, which by itself is immunogenic, did not pro

9 97.3%) Actinomyces strains, 42 of 46 (91.3%) B. fragilis group strains, 79 of 103 (76.7%) Clostridium

10 Of 382 B. fragilis-positive specimens, 14.4% of the strains fou

11 analysis of the PS A biosynthesis loci of 50 B. fragilis isolates indicates that regions flanking eac

12 A collection of 50 B. fragilis strains was examined.

13 used to amplify the region from 45 of the 50 B. fragilis strains studied.

14 mylase indicates that amino acid residue 90 (B. fragilis numbering) plays an important role in confer

15 ributes to sepsis in mice, and we identify a B. fragilis protease called fragipain (Fpn) that is requ

16 o lethal disease from ETBF colonization in a B. fragilis toxin (BFT)-dependent manner.

17 NAc as the sole carbon source, we isolated a B. fragilis mutant strain that can grow on this substrat

18 Although B. fragilis does not normally grow with manNAc as the so

19 An increase in recovery of E. coli and B. fragilis was noted in patients with bowel or bladder

20 al conjugation experiments using E. coli and B. fragilis.

21 a scan of the Flavobacterium johnsoniae and B. fragilis genomes for putative promoters, resulting in

22 C. perfringens and B. fragilis provided moderate synergy to each other but

23 ay an altered serum metabolomic profile, and B. fragilis modulates levels of several metabolites.

24 re those of Bacteroides thetaiotaomicron and B. fragilis.

25 By this approach, B. fragilis enterotoxin gene sequences were detected in

26 aling that Tn5520 mobilizes plasmids in both B. fragilis and Escherichia coli at high frequency and a

27 ction or commensalism, induction of IL-10 by B. fragilis is critical to this microbe's interactions w

28 Whether the virulence mechanisms employed by B. fragilis during infections differ from those employed

29 zation of the gnotobiotic mouse intestine by B. fragilis requires that the organism synthesize only a

30 ite that is increased by MIA and restored by B. fragilis causes certain behavioral abnormalities, sug

31 Only cfiA(+) B. fragilis strains, which represent 3% of the clinical

32 ize microbial biogeography within the colon, B. fragilis penetrates the colonic mucus and resides dee

33 nted indicate that during aerobic conditions B. fragilis NrdAB may have a role in maintaining deoxyri

34 constitution with polysaccharide A-deficient B. fragilis restored EAE susceptibility.

35 When intestinal integrity is disrupted, B. fragilis and colonic contents escape into the periton

36 he immunomodulatory activities of PSA during B. fragilis colonization include correcting systemic T c

37 MRP cells cocultured with either B. fragilis CPC of LPS in vitro produced tumor necrosis

38 and those that do are called enterotoxigenic B. fragilis (ETBF).

39 Fpn-deficient, enterotoxigenic B. fragilis has an attenuated ability to induce sepsis i

40 ated with diarrheal disease (enterotoxigenic B. fragilis) produce a 20-kDa zinc-dependent metalloprot

41 agilis toxin gene (bft) from enterotoxigenic B. fragilis (ETBF) 86-5443-2-2 is reported.

42 ssay can be used to identify enterotoxigenic B. fragilis and may be used clinically to determine the

43 hogenicity island (BfPAI) in enterotoxigenic B. fragilis (ETBF) strain 86-5443-2-2 and a related gene

44 ly known virulence factor of enterotoxigenic B. fragilis.

45 ces or 100 to 1,000 cells of enterotoxigenic B. fragilis/g of stool.

46 diarrhea in children (termed enterotoxigenic B. fragilis, or ETBF) produce a heat-labile ca. 20-kDa p

47 er, the cloning and sequencing of the entire B. fragilis toxin gene (bft) from enterotoxigenic B. fra

48 Deletion of its gene resulted in the first B. fragilis mutant able to synthesize only one phase-var

49 al homologs, allow corrected annotations for B. fragilis bfr and other dpsl genes within the bacteria

50 t Bacteroidales strains analyzed, except for B. fragilis strains with the same T6SS locus.

51 Notably, the CCF system is required for B. fragilis colonization following microbiome disruption

52 TLR2 on CD4(+) T cells is required for B. fragilis colonization of a unique mucosal niche in mi

53 Our findings define a role for B. fragilis enterotoxin and its activating protease in t

54 ociated and complex colonized mice, and from B. fragilis-induced murine abscesses.

55 Tn5520 was captured from B. fragilis LV23 by using the transfer-deficient shuttle

56 new, self-transferable transfer factor from B. fragilis LV23 and that this new factor encodes a tetr

57 with the promoter regions of nqrA genes from B. fragilis and Vibrio cholerae.

58 cLV25 was isolated from B. fragilis LV25 by its capture on the nonmobilizable Es

59 dynamics of the metallo-beta-lactamase from B. fragilis have been examined using (15)N NMR relaxatio

60 ese data indicate that a purified toxin from B. fragilis strains associated with diarrhea rapidly and

61 biotic colonization of mammals, we generated B. fragilis strains deleted in the global regulator of p

62 Among the Bacteroides spp. in the gut, B. fragilis has the unique ability of efficiently harves

63 In animals harbouring B. fragilis not expressing PSA, H. hepaticus colonizatio

64 In B. fragilis, protein glycosylation is a fundamental and

65 to 5-nitroimidazole antimicrobial agents in B. fragilis strains from Europe and Africa.

66 supports lipopolysaccharide biosynthesis in B. fragilis and is subject to feedback regulation by CMP

67 sible for lipopolysaccharide biosynthesis in B. fragilis.

68 g them able to induce abscesses is common in B. fragilis.

69 te-specific recombinase family, conserved in B. fragilis, mediate additional DNA inversions of the B.

70 psis in mice; however, Fpn is dispensable in B. fragilis colitis, wherein host proteases mediate BFT

71 a single gene, oxe (i.e., oxygen enabled) in B. fragilis allows for growth in concentrations as high

72 brings the total of oxyR-controlled genes in B. fragilis to five and suggests the existence of a seco

73 ence of two distinct subfamilies of GH110 in B. fragilis and thetaiotaomicron strains.

74 putative promoters, resulting in 188 hits in B. fragilis and 109 hits in F. johnsoniae.

75 ive function, associated with an increase in B. fragilis.

76 scriptional regulator and may be involved in B. fragilis peroxide resistance.

77 e global regulatory nature of the process in B. fragilis suggest an evolutionarily ancient mechanism

78 an important role in peroxide resistance in B. fragilis.

79 further our understanding of DNA transfer in B. fragilis, we isolated and characterized a new transfe

80 required for GALT development, we introduced B. fragilis along with stress-response mutants of B. sub

81 the distribution and colonization of labeled B. fragilis along the intestine, as well as niche compet

82 sorption studies demonstrate that the native B. fragilis enzyme tightly binds 2 mol of Zn(II) and, al

83 Neither B. fragilis ATCC 25285(pFD340-prtP) cells nor the CHAPS

84 rates of isolation of B. fragilis versus non-B. fragilis species had an overall effect on susceptibil

85 rom DNA extracted from 28 nonenterotoxigenic B. fragilis isolates or B. distasonis, B. thetaiotaomicr

86 cate that whereas both ETBF and nontoxigenic B. fragilis (NTBF) chronically colonize mice, only ETBF

87 udying a collection of ETBF and nontoxigenic B. fragilis (NTBF) strains, we found that bft and a seco

88 ins that do not secrete BFT are nontoxigenic B. fragilis (NTBF), and those that do are called enterot

89 can be transferred from ETBF to nontoxigenic B. fragilis strains by a mechanism similar to that for t

90 lla pneumoniae (serotypes O1, O2ab, and O3), B. fragilis, and Bacteroides vulgatus.

91 symbiosis factor (PSA, polysaccharide A) of B. fragilis signals through TLR2 directly on Foxp3(+) re

92 any of these genes eliminates the ability of B. fragilis to grow on NANA.

93 tive stress are physiological adaptations of B. fragilis to its environment that enhance survival in

94 the role of the CPC in promoting adhesion of B. fragilis to the peritoneal wall and coordinating the

95 ress response important for aerotolerance of B. fragilis.

96 0', enabling rapid isolation and analysis of B. fragilis mutants has been constructed.

97 There are 2 classes of B. fragilis distinguished by their ability to secrete a

98 We studied the dissemination of B. fragilis after acute peritonitis and characterized th

99 nts were designed to determine the effect of B. fragilis enterotoxin on bacteria-enterocyte interacti

100 nested PCR to detect the enterotoxin gene of B. fragilis in stool specimens.

101 The ccf genes of B. fragilis are upregulated during gut colonization, pre

102 e identified 1021 candidate glycoproteins of B. fragilis.

103 The pathogenicity islet of B. fragilis VPI 13784 was defined as 6,033 bp in length

104 Clinical isolates of B. fragilis displayed a greater capacity for growth unde

105 ich represent 3% of the clinical isolates of B. fragilis, displayed heterogeneity in the regions flan

106 This change in rates of isolation of B. fragilis versus non-B. fragilis species had an overal

107 with that of the PS B1 biosynthesis locus of B. fragilis NCTC 9343.

108 Three-dimensional modeling of B. fragilis Omp121 (based on 1D and 3D sequence profiles

109 A PSA mutant of B. fragilis does not restore these immunologic functions

110 and may explain the abscessogenic nature of B. fragilis.

111 dies against CTLA-4 favored the outgrowth of B. fragilis with anticancer properties.

112 could also contribute to the pathogenesis of B. fragilis in extraintestinal infections.

113 of these findings for the pathophysiology of B. fragilis in extraintestinal infections and competitio

114 In this study, we analysed the phenotype of B. fragilis mutants with defective protein glycosylation

115 lycosylation is central to the physiology of B. fragilis and is necessary for the organism to competi

116 , one of the two capsular polysaccharides of B. fragilis 9343.

117 The capsular polysaccharides of B. fragilis are part of a complex of surface polysacchar

118 relation between disease and the presence of B. fragilis enterotoxin.

119 in the number of IRs are active processes of B. fragilis in the endogenous human intestinal ecosystem

120 han half of the extracytoplasmic proteins of B. fragilis, are glycosylated.

121 ulatory molecule, polysaccharide A (PSA), of B. fragilis mediates the conversion of CD4(+) T cells in

122 be used clinically to determine the role of B. fragilis in diarrheal diseases.

123 plots of the deduced amino acid sequence of B. fragilis Omp121 display striking similarity with thos

124 T-ETBF) strains, a nontoxigenic WT strain of B. fragilis (WT-NTBF), WT-NTBF overexpressing bft (rETBF

125 estinal recolonization with either strain of B. fragilis.

126 Enterotoxigenic strains of B. fragilis have been associated with diarrheal diseases

127 e epidemiology of enterotoxigenic strains of B. fragilis in clinical infections and whether there is

128 t and that addAB is required for survival of B. fragilis following DNA damage.

129 polysaccharides, or by adoptive transfer of B. fragilis-specific T cells.

130 Unlike the other described Tsrs of B. fragilis, Tsr19 brings about inversion of two DNA reg

131 esponses specific for B. thetaiotaomicron or B. fragilis were associated with the efficacy of CTLA-4

132 of MPII and FRA is required for the overall B. fragilis virulence in vivo.

133 e for commensal bacterial Ags, in particular B. fragilis expressing polysaccharide A, in protecting a

134 Enterotoxigenic strains that produce B. fragilis toxin (BFT, fragilysin) contribute to coliti

135 e studied the effects of the highly purified B. fragilis fragilysin on the barrier function of cultur

136 We now demonstrate that purified B. fragilis toxin (BFT) up-regulates SMO in HT29/c1 and

137 2.5 mM, while those of Bacteroides pyogenes, B. fragilis, and Akkermansia muciniphila were greater in

138 To chart the evolution of the more reactive B. fragilis enzyme, we have made changes in an active si

139 f the possibility of metronidazole-resistant B. fragilis strains in the United States and the importa

140 ETBF that secretes B. fragilis toxin (BFT) causes human inflammatory diarrh

141 In this study, the six B. fragilis thioredoxins (Trxs) were investigated to det

142 A soluble B. fragilis metallo-beta-lactamase has been purified to

143 egions of GA3 loci, GA3 T6SSs of the species B. fragilis are likely the source of numerous novel effe

144 polysaccharide complex of a commonly studied B. fragilis strain, 638R, that is distinct from strain 9

145 roximately 6-kb pathogenicity island (termed B. fragilis pathogenicity island or BfPAI) which is pres

146 cretes a 20-kDa metalloprotease toxin termed B. fragilis toxin (BFT).

147 ca. 20-kDa heat-labile protein toxin (termed B. fragilis toxin [BFT]) have been associated with diarr

148 Our results therefore demonstrate that B. fragilis co-opts the Treg lineage differentiation pat

149 Here we demonstrate that B. fragilis encodes a cytochrome bd oxidase that is esse

150 These results imply that B. fragilis has two pathways for alpha-ketoglutarate bio

151 e deduced amino acid sequences revealed that B. fragilis AhpCF shares approximately 60% identity to o

152 otting and oxyR'::xylB fusions revealed that B. fragilis OxyR does not control its own expression.

153 Our studies show that B. fragilis fragilysin alters the barrier function of th

154 ansferrin as a model, it has been shown that B. fragilis alone can rapidly and efficiently deglycosyl

155 The data provided herein suggest that B. fragilis uses N-succinyl-L-ornithine rather than N-ac

156 The B. fragilis nrdA and nrdB genes were overexpressed in Es

157 The B. fragilis protein profile was significantly altered af

158 The B. fragilis toxin (bft) gene from ETBF strain 86-5443-2-

159 The bft gene and the B. fragilis pathogenicity island (BfPAI) were cloned int

160 D Q5LIW1) is the only protein encoded by the B. fragilis genome with significant identity to any know

161 alloproteinase II (MPII)) are encoded by the B. fragilis pathogenicity island.

162 in conferring charged groups that enable the B. fragilis capsular polysaccharides to induce abscesses

163 %) differ greatly from that reported for the B. fragilis chromosome (42%), suggesting that the BfPAI

164 ce in time to detection was greatest for the B. fragilis group: FN, 28 h, versus SN, 60.0 h.

165 e proposed minimum kinetic mechanism for the B. fragilis metallo-beta-lactamase-catalyzed nitrocefin

166 egions flanking the Tn4400' insertion in the B. fragilis chromosome revealed the presence of five ope

167 e bacterioferritin-related (bfr) gene in the B. fragilis oxidative stress response.

168 ight into the role of individual Trxs in the B. fragilis oxidative stress response.

169 ly, by introducing a C104R mutation into the B. fragilis enzyme, binding of two zinc ions is maintain

170 ng power equivalents to the periplasm of the B. fragilis cell.

171 be complemented by a 6.6 kb fragment of the B. fragilis chromosome.

172 obtained which was confirmed as part of the B. fragilis enterotoxin gene by Southern blotting with a

173 uxs1 is located in a conserved region of the B. fragilis genome, whereas Bfuxs2 is in the heterogeneo

174 is, mediate additional DNA inversions of the B. fragilis genome.

175 Analysis of the B. fragilis genomic sequence, together with genetic cons

176 mation on hand, a catalytic mechanism of the B. fragilis metallo-beta-lactamase is proposed.

177 tively, the two mobilization proteins of the B. fragilis mobilizable transposon Tn4399.

178 was found to be present in the genome of the B. fragilis NCTC9343 strain but absent in strains 638R,

179 report the cloning and overexpression of the B. fragilis phosphonopyruvate decarboxylase gene (aepY),

180 the protein profile of crude extracts of the B. fragilis strains revealed that at least three oxidati

181 ory region was also observed upstream of the B. fragilis superoxide dismutase gene sod.

182 Expression of the B. fragilis trxB gene was induced following treatment wi

183 ETBF was isolated from 20.3% (40/197) of the B. fragilis-positive diarrheal specimens and from 8.1% (

184 heal specimens and from 8.1% (15/185) of the B. fragilis-positive nondiarrheal specimens (P < .001) a

185 islet is inserted at a specific site on the B. fragilis chromosome.

186 isolation of the various species showed the B. fragilis species comprised 58% of the isolates in 198

187 c-dependent metalloprotease toxin termed the B. fragilis toxin (BFT) have been associated with acute

188 Homology searches indicated that the B. fragilis aconitase is most closely related to aconita

189 at they are functional paralogs and that the B. fragilis NCTC 9343 PSF repeat unit contains xylose.

190 etic calculations have demonstrated that the B. fragilis protein efficiently binds the internal Na(+)

191 subconfluent HT29/C1 cells treated with the B. fragilis toxin (BFT) develop morphologic changes with

192 Thus, B. fragilis possesses a new pathway of NANA utilization,

193 -independent IL-10 production in response to B. fragilis during its pathogenic interactions with the

194 raabdominal abscess formation in response to B. fragilis.

195 E. coli provided strong synergy to B. fragilis but not to C. perfringens.

196 udied the expression of bft in non-toxigenic B. fragilis (NTBF) strains.

197 20-kDa zinc-dependent metalloprotease toxin (B. fragilis enterotoxin; BFT) that reversibly stimulates

198 rete a zinc-dependent metalloprotease toxin, B. fragilis toxin (BFT).

199 ained from mice reconstituted with wild type B. fragilis had significantly enhanced rates of conversi

200 Recolonization with wild type B. fragilis maintained resistance to EAE, whereas recons

201 l abscesses induced by challenge with viable B. fragilis.

202 rum reactivity against Bacteroides vulgatus, B. fragilis, Prevotella intermedia, and, to a lesser ext

203 nsferrin deglycosylation occurs in vivo when B. fragilis is propagated in the rat tissue cage model o

204 We identify an alternative pathway by which B. fragilis is able to reestablish capsule production an

205 Monocolonization of germ-free animals with B. fragilis increases the suppressive capacity of Tregs

206 o develop abscesses following challenge with B. fragilis or abscess-inducing zwitterionic polysacchar

207 Upon challenge with B. fragilis, mortality rates and serum proinflammatory c

208 This defect was overcome by gavage with B. fragilis, by immunization with B. fragilis polysaccha

209 9 enterocytes were incubated for 1 hour with B. fragilis enterotoxin, followed by 1 hour of incubatio

210 avage with B. fragilis, by immunization with B. fragilis polysaccharides, or by adoptive transfer of

211 o was increased in YAMC cells incubated with B. fragilis, an effect mediated by lipopolysaccharide an

212 sphingolipids, we found that treatment with B. fragilis glycosphingolipids-exemplified by an isolate