ライフサイエンスコーパス: B. henselae

1 B. henselae and B. quintana, the organisms that cause ba

2 B. henselae bacteremia was detected in 89% of the 47 nat

3 B. henselae can be difficult to culture axenically, and

4 B. henselae DNA was detected in 34% of 132 fleas, with s

5 B. henselae DNA was identified in 27 of 42 (64%) histolo

6 B. henselae induction of VEGF, IL-1beta, and IL-8 outlin

7 B. henselae target DNA was amplified from 100% of sample

8 B. henselae was identified in 8 of 10 serologically posi

9 B. henselae, B. quintana, and C. burnetii seropositivity

10 B. henselae-mediated inhibition of apoptosis, as indicat

11 A total of 118 B. henselae strains were delineated into 12 sequence typ

12 roup 1), 10(8) (group 2), or 10(6) (group 3) B. henselae or with saline (group 4) or were not inocula

13 The 16S rRNA type was determined for 49 B. henselae isolates.

14 o gfp were examined by flow cytometry, and a B. henselae groEL promoter fusion which induced expressi

15 We identified a B. henselae transposon mutant that constitutively expres

16 All B. henselae-inoculated cats were bacteremic by 2 weeks a

17 onii subsp. berkhoffii, B. clarridgeiae, and B. henselae), highly suggestive of Bartonella endocardit

18 Early recognition of HLH and B. henselae through liver biopsy and serological tests l

19 cat had high Bartonella antibody titers and B. henselae type I DNA was detected in the damaged aorti

20 pattern of ocular disease in AIDS-associated B. henselae infections is poorly delineated; unusual man

21 Our study strongly associates B. henselae with cat scratch disease, suggesting that it

22 pecies (i.e., B. vinsonii subsp. berkhoffii, B. henselae, and B. clarridgeiae) that are currently rec

23 microvascular endothelial cells (HMEC-1) by B. henselae resulted in the formation of well-defined va

24 ompare it to infections with those caused by B. henselae and B. clarridgeiae.

25 Infection of human endothelial cells by B. henselae resulting in IL-8 production likely plays a

26 ression appears to be a strategy employed by B. henselae to survive in the arthropod vector and the m

27 tients > 4 weeks into illness) and confirmed B. henselae as the causative species.

28 We designed a study to detect B. henselae in archival biopsies by polymerase chain rea

29 cell is recruited to the endothelium during B. henselae infection and then contributes to bacterial-

30 ontributing to the angiogenic process during B. henselae infection by infiltrating BA lesions and sec

31 nfestation was a significant risk factor for B. henselae bacteremia (odds ratio = 2.82, 95% confidenc

32 California were significant risk factors for B. henselae seropositivity.

33 ith 21.2% of sera from patients positive for B. henselae immunoglobulin G antibodies by indirect immu

34 e as a previously unrecognized reservoir for B. henselae.

35 ia, and 138 (51%) cats were seropositive for B. henselae.

36 -like factor autotransporter gene (cfa) from B. henselae.

37 on of an antigenic autotransporter gene from B. henselae.

38 lture was determined with blood samples from B. henselae-infected cats.

39 Blood specimens from B. henselae-infected cats were collected in both EDTA an

40 Furthermore, supernatants from B. henselae-infected HMEC-1 were able to induce chemotax

41 nselae with variations in the 16S rRNA gene, B. henselae type I and type II.

42 Heterogeneous B. henselae-specific IgG reactivity with numerous protei

43 We also investigated the role of IL-8 in B. henselae-induced endothelial cell proliferation and c

44 ransposon mutagenesis to screen for genes in B. henselae heme binding and uptake pathways.

45 Overexpression of hbpC in B. henselae increased resistance to heme toxicity, impli

46 ein (GFP) gene was expressed on a plasmid in B. henselae, and GFP-expressing bacteria were visualized

47 at a homologue of this antigen is present in B. henselae.

48 17-kDa antigen gene, which replaces virB5 in B. henselae, was also demonstrated at the protein level

49 In the immunocompromised individual, B. henselae-induced angiogenesis, or bacillary angiomato

50 most often responsible for human infection, B. henselae and B. quintana, cause prolonged febrile ill

51 Adherence of intact B. henselae to HUVECs was inhibited by increasing concen

52 in (GFP) gene (gfpmut3) and transformed into B. henselae by electroporation.

53 BclI-EcoRI DNA fragment expresses a 120-kDa B. henselae protein immunoreactive with 21.2% of sera fr

54 erns were variable, one approximately 83-kDa B. henselae protein (Bh83) was immunoreactive with all C

55 mice and was reactive with rabbit anti-live B. henselae and mouse anti-Pap31 antibodies by Western b

56 ated human THP-1 macrophages exposed to live B. henselae.

57 The ability to model B. henselae invasion of feline RBC in vitro should permi

58 n and deletion of hbpC affect the ability of B. henselae to infect the cat host.

59 n this study, we investigated the ability of B. henselae to upregulate MCP-1 gene expression and prot

60 of Escherichia coli or the 43-kDa antigen of B. henselae.

61 om the cat were reactive against antigens of B. henselae (titer, 1,024), B. quintana (titer, 128), an

62 and parenchymal peliosis, and in the case of B. henselae cat-scratch disease.

63 contribution of antibodies to the control of B. henselae in cats.

64 osplenic cat scratch disease by detection of B. henselae DNA in the orbital abscess fluid.

65 ther improve the sensitivity of detection of B. henselae.

66 lonization that promote the establishment of B. henselae infections in vivo.

67 Promoter fusions of B. henselae chromosomal DNA to gfp were examined by flow

68 Thus, expression of the virB genes of B. henselae is induced in bacteria, which have invaded h

69 n this study we examined the interactions of B. henselae Pap31 with fibronectin (Fn), heparin (Hep),

70 than tubes containing EDTA for isolation of B. henselae and suggest that, for cat blood, collection

71 A cosmid library of B. henselae ATCC 49793 was constructed using SuperCos1 i

72 Frozen blood yielded the largest number of B. henselae colonies for any of the schedules tested.

73 licting reports describe the pathogenesis of B. henselae in the cat.

74 a role for microglia in the pathogenesis of B. henselae-associated neurological disease.

75 were tested individually for the presence of B. henselae DNA by PCR.

76 to determine the longitudinal prevalence of B. henselae bacteremia, the prevalence of B. henselae in

77 of B. henselae bacteremia, the prevalence of B. henselae in the fleas infesting these cats, and wheth

78 toxicity, implicating HbpC in protection of B. henselae from the toxic levels of heme present in the

79 reted cohemolysin autotransporter protein of B. henselae.

80 passive antibody to the homologous strain of B. henselae did not prevent bacteremia.

81 at B. henselae LSU16 is a virulent strain of B. henselae in cats and propose that the virulence of B.

82 In this study, we characterized a strain of B. henselae termed LSU16.

83 s (0.5%) were coinfected with two strains of B. henselae with variations in the 16S rRNA gene, B. hen

84 pling to explore the population structure of B. henselae in the United Kingdom and to determine the d

85 understand better the long-term survival of B. henselae in cats, we examined the feline humoral immu

86 In the wild-type strain, transcription of B. henselae hbpC was upregulated at arthropod temperatur

87 oxacin may prove useful for the treatment of B. henselae infections.

88 ae in cats and propose that the virulence of B. henselae in cats is strain dependent.

89 omatosis, but immunohistochemistry ruled out B. henselae and B. quintana.

90 om naturally infected cats was used to probe B. henselae total membranes to detect commonly recognize

91 , 2, 4, and 2 were positive for B. quintana, B. henselae, and C. burnetii, respectively, by the dPCR

92 e cell lysate fractions from closely related B. henselae, although possessing significant mitogenicit

93 etected infection with a Bartonella species (B. henselae or B. vinsonii subsp. berkhoffii) in blood s

94 Two species, B. henselae and B. quintana, have been associated with b

95 e eye by Parinaud's oculoglandular syndrome; B. henselae is the most common cause.

96 We conclude that B. henselae LSU16 is a virulent strain of B. henselae in

97 I found that B. henselae induces long-term endothelial survival and t

98 We hypothesize that B. henselae-mediated interaction with immune cells, name

99 , two-dimensional immunoblots indicated that B. henselae LPS and members of the Hbp family of protein

100 This study indicates that B. henselae or B. clarridgeiae can induce chronic infect

101 One hypothesis for this discrepancy is that B. henselae strains vary in their zoonotic potential.

102 Previously, it has been shown that B. henselae infection can result in production of the ch

103 Pap31 is an Fn-binding protein mediating the B. henselae-host interaction(s), and they implicate the

104 Sequencing of the region upstream of the B. henselae virB2 gene revealed a region with sequence h

105 d that HbpC binds hemin and localizes to the B. henselae outer membrane and outer membrane vesicles.

106 elizabethae, 12.5%; to B. quintana, 9.5%; to B. henselae, 3.5%; to Seoul virus, 0.5%; and to Ricketts

107 B. koehlerae was more closely related to B. henselae than to B. clarridgeiae by protein profile,

108 and hepatocytes produce IL-8 in response to B. henselae infection.

109 amined the feline humoral immune response to B. henselae outer membrane (OM) proteins in naturally an

110 cats developed strong antibody responses to B. henselae, as determined by Western blot analysis and

111 moterless vector pANT3 and used to transform B. henselae.

112 onstrate that the cat flea readily transmits B. henselae to cats.

113 ved from bacteremic cattery cats transmitted B. henselae to five SPF kittens in two separate experime

114 rther revealed similar banding patterns when B. henselae was reacted against the Ig isotypes IgG and

115 the fleas infesting these cats, and whether B. henselae is transmitted experimentally to cats via fl

116 is study was undertaken to determine whether B. henselae infects feline fetal brain cells in vitro.

117 ens should elucidate the mechanisms by which B. henselae establishes persistent bacteremic infections

118 Cats bacteremic with B. henselae constitute a large reservoir from which huma

119 Experimental inoculation of cats with B. henselae strains demonstrated that both constitutive

120 hermore, infection of endothelial cells with B. henselae stimulated upregulation of the IL-8 chemokin

121 sampled, 5 cats (1.1%) were coinfected with B. henselae and B. clarridgeiae and 2 cats (0.5%) were c

122 te and OMPs from B. koehlerae, compared with B. henselae and B. clarridgeiae.

123 osis hepatis was associated exclusively with B. henselae.

124 peliosis, 26 (53 percent) were infected with B. henselae and 23 (47 percent) with B. quintana.

125 Cats were readily infected with B. henselae by intravenous inoculation, developed histop

126 ved epitope expression during infection with B. henselae or B. quintana.

127 s epitope conservation during infection with B. henselae or B. quintana.

128 ocyte-enriched cultures were inoculated with B. henselae.

129 Patients with B. henselae infection were identified throughout the stu

130 iters, all eight cats were rechallenged with B. henselae.