ライフサイエンスコーパス: B. parapertussis

1 trisaccharide plus an O-antigen-like repeat (B. parapertussis).

2 d whooping cough in humans (B. pertussis and B. parapertussis).

3 s and B. holmesii and 68% and 72% identified B. parapertussis.

4 bronchiseptica (RB50), and other isolates of B. parapertussis.

5 f B. pertussis and B. bronchiseptica but not B. parapertussis.

6 r strains of B. pertussis and two strains of B. parapertussis.

7 lla parapertussis and cloned part of it from B. parapertussis.

8 positive samples, 13.99% were identified as B. parapertussis.

9 isolates, which were positive with IS1001 of B. parapertussis.

10 related pathogens, Bordetella pertussis and B. parapertussis.

11 cacy of the acellular vaccine Adacel against B. parapertussis.

12 ot efficiently mediate opsonophagocytosis of B. parapertussis.

13 tection against B. pertussis but not against B. parapertussis.

14 factor is a potential protective antigen for B. parapertussis.

15 e surface and complement-mediated killing of B. parapertussis.

16 on of two palmitate acyl chains is unique to B. parapertussis.

17 larger than that induced by B. pertussis or B. parapertussis.

18 he human-adapted subspecies B. pertussis and B. parapertussis.

19 etella bronchiseptica and the human pathogen B. parapertussis.

20 RB50 (5,338,400 bp; 5,007 predicted genes), B. parapertussis 12822 (4,773,551 bp; 4,404 genes) and B

21 These are the first LPS mutants generated in B. parapertussis and B. bronchiseptica and the first dee

22 Resistance is not efficiently acquired by B. parapertussis and B. bronchiseptica mutants lacking O

23 ers to PT, we examined the ptx genes of both B. parapertussis and B. bronchiseptica to determine whet

24 We have found that B. parapertussis and B. bronchiseptica, unlike B. pertus

25 Our analysis indicates that B. parapertussis and B. pertussis are independent deriva

26 ns of cytokines involved in the clearance of B. parapertussis and immunomodulation that delays effect

27 antigen is a critical protective antigen of B. parapertussis and its inclusion can substantially imp

28 differentiate B. pertussis, B. holmesii, and B. parapertussis and provided protocols and training to

29 mens, 12 were positive (9 B. pertussis and 3 B. parapertussis) and 68 specimens were negative by all

30 Bordetella bronchiseptica, B. pertussis, and B. parapertussis) and its role in their biofilm developm

31 spp., including 4 of B. bronchiseptica, 5 of B. parapertussis, and 5 of B. pertussis, were studied.

32 genes are highly conserved in B. pertussis, B. parapertussis, and B. avium.

33 Bordetella pertussis, B. parapertussis, and B. bronchiseptica are closely rela

34 ecies specificities of Bordetella pertussis, B. parapertussis, and B. bronchiseptica might be explain

35 eptica cluster, which includes B. pertussis, B. parapertussis, and B. bronchiseptica.

36 apid identification of Bordetella pertussis, B. parapertussis, and B. holmesii was developed using mu

37 trisaccharide on the LPS core is present in B. parapertussis, and further suggests that the wild-typ

38 d in B. pertussis and B. holmesii; IS1001 of B. parapertussis; and the IS1001-like sequence of B. hol

39 the toxins encoded by B. bronchiseptica and B. parapertussis are active.

40 Although both B. pertussis and B. parapertussis are more closely related to B. bronchis

41 Here we show that B. pertussis and B. parapertussis are predominantly differentiated from B

42 pertussis Tohama I, B. pertussis 18-323, and B. parapertussis ATCC 15311.

43 Forty-eight hours after infection, wild-type B. parapertussis bacteria but not the O antigen-deficien

44 ng B. bronchiseptica but not B. pertussis or B. parapertussis bacterial numbers during the first 72 h

45 The passive transfer of sera raised against B. parapertussis, but not B. parapertussis Deltawbm, red

46 Interestingly, B. parapertussis, but not B. pertussis, produces an O an

47 B. parapertussis can also cause whooping cough, and B. b

48 ed efficient protection against a subsequent B. parapertussis challenge.

49 Thus, the O antigen of B. parapertussis confers asymmetrical cross-immunity bet

50 n, and found that both B. bronchiseptica and B. parapertussis contain at least certain of these genes

51 B. parapertussis contains a putative pagP homolog (encod

52 In B. bronchiseptica and B. parapertussis, delta wlb mutants also lacked O-antige

53 (LPS), which contains the O antigen, but not B. parapertussis Deltawbm LPS drastically improved the e

54 The B. parapertussis Deltawbm mutant was severely defective

55 th an isogenic mutant lacking the O antigen, B. parapertussis Deltawbm, induced antibodies that recog

56 era raised against B. parapertussis, but not B. parapertussis Deltawbm, reduced B. parapertussis load

57 B. pertussis and B. parapertussis Deltawlb mutants were also defective co

58 commercial laboratories for B. pertussis and B. parapertussis detection.

59 Since B. parapertussis did not cause severe disease in IL-1R(-

60 In contrast, LpxA from B. parapertussis did not display relaxed specificity but

61 tingly, serum antibody-mediated clearance of B. parapertussis did not require Fc receptors that are r

62 ase in TLR4-deficient mice, B. pertussis and B. parapertussis do not.

63 el of infection, immunization with wild-type B. parapertussis elicited a strong antibody response to

64 tica has a wide host range, B. pertussis and B. parapertussis evolved separately from a B. bronchisep

65 itated the simple and effective isolation of B. parapertussis from ovine nasal swabs and, in successf

66 n of leukocytes in lungs and in clearance of B. parapertussis from the lungs.

67 d as an improved selective medium to isolate B. parapertussis from the nasal cavities of conventional

68 Bordetella pertussis, B. bronchiseptica, and B. parapertussis genome assemblies permitted the identif

69 The increasing incidence of B. parapertussis has been attributed to the lack of cros

70 . pertussis have been well studied, those of B. parapertussis have not.

71 ctious bordetellae, Bordetella pertussis and B. parapertussis, have emerged in historical times as co

72 te according to pathogen host range and that B. parapertussis(hu) most likely acquired its fhaS allel

73 Notably, the genes present in B. parapertussis(hu) strains were pseudogenes, and the g

74 ludes both human-infective (B. pertussis and B. parapertussis(hu)) and non-human-infective (B. bronch

75 e of human-derived Bordetella parapertussis (B. parapertussis(hu)) contains a large in-frame deletion

76 owing inoculation with B. pertussis, but not B. parapertussis, IL-1R(-/-) mice showed elevated bacter

77 (Deltawbm) mutants of B. bronchiseptica and B. parapertussis in a variety of assays relevant to natu

78 Attempts to assess the prevalence of B. parapertussis in conventionally reared sheep by nasal

79 ovement in the detection of B. pertussis and B. parapertussis in nasopharyngeal specimens.

80 arrant investigation of the relative role of B. parapertussis in the resurgence of whooping cough.

81 adapting to infect humans, B. pertussis and B. parapertussis independently modified their LPS to red

82 Together, these data indicate that B. parapertussis induces the production of IL-10, which

83 ronchiseptica, while no role for TLR4 during B. parapertussis infection has been described.

84 B. pertussis vaccines have little effect on B. parapertussis infection or disease suggest that the p

85 Immunity induced by B. parapertussis infection protected against subsequent

86 In addition, nine cases of B. parapertussis infection were also confirmed by using

87 It was concluded that B. parapertussis infections are more common than previou

88 After finding that several children with B. parapertussis infections exhibited modest antibody ti

89 ussis infections but did not protect against B. parapertussis infections.

90 The O antigen of B. parapertussis inhibited binding of antibodies to the

91 Here, we evaluated the outcome of B. parapertussis innate interaction with human macrophag

92 It was previously shown that B. parapertussis is able to avoid bacterial killing by p

93 resent study explores the mechanism by which B. parapertussis is cleared from the lower respiratory t

94 pectrometry analysis revealed that wild-type B. parapertussis lipid A consists of a heterogeneous mix

95 The addition of 10 microg of purified B. parapertussis lipopolysaccharide (LPS), which contain

96 , but not B. parapertussis Deltawbm, reduced B. parapertussis loads in the lower respiratory tracts o

97 0-fold more stimulatory than B. pertussis or B. parapertussis LPS, respectively.

98 ocytes accumulated in the lungs, and cleared B. parapertussis more rapidly.

99 These results highlight the need for B. parapertussis opsonic antibodies to induce bacterial

100 s were pseudogenes, and the genes present in B. parapertussis(ov) strains were expressed at significa

101 d non-human-infective (B. bronchiseptica and B. parapertussis(ov)) strains.

102 s contains a putative pagP homolog (encoding B. parapertussis PagP [PagPBPa]), but its role in the bi

103 ations in the locus in B. bronchiseptica and B. parapertussis prevent O-antigen biosynthesis and prov

104 The toxin encoded by the B. parapertussis ptx genes appeared more labile in cultu

105 The B. bronchiseptica and B. parapertussis recipients were now able to biosynthesi

106 lymerase chain reaction results positive for B. parapertussis reported during October 2011-May 2012 w

107 quences IS481 and IS1001 of B. pertussis and B. parapertussis, respectively, and is performed using t

108 umulation of CD4(+) T cells in the lungs and B. parapertussis-responsive IFN-gamma-producing cells in

109 us wlb locus of Bordetella bronchiseptica or B. parapertussis restored partial sensitivity to Ba1.

110 tussis does not express the O antigen, while B. parapertussis retains it as a dominant surface antige

111 ve strains of B. pertussis and one strain of B. parapertussis revealed extensive divergence of gene o

112 rtussis strain 18323 and an ovine isolate of B. parapertussis show significant transcription of the g

113 In vitro B. parapertussis-stimulated macrophages produced IL-10,

114 ed efficacy of B. pertussis vaccines against B. parapertussis suggest a lack of cross-protective immu

115 eir control and clearance of B. pertussis or B. parapertussis, suggesting that IgA is not crucial to

116 t that in the absence of opsonic antibodies, B. parapertussis survives inside macrophages by preventi

117 that might be misclassified as pertussis if B. parapertussis testing is not performed.

118 large genetic locus in B. bronchiseptica and B. parapertussis that is required for O-antigen biosynth

119 for the mechanism of protective immunity to B. parapertussis that is similar but distinct from that

120 o the bacterial surface and was required for B. parapertussis to colonize mice convalescent from B. p

121 that O antigen contributes to the ability of B. parapertussis to colonize the respiratory tract durin

122 These data indicate that O antigen enables B. parapertussis to efficiently colonize the lower respi

123 in the absence of opsonins, O antigen allows B. parapertussis to inhibit phagolysosomal fusion and to

124 The O antigen targets B. parapertussis to lipid rafts that are retained in the

125 In addition, O antigen was required for B. parapertussis to systemically spread in complement-su

126 In other assays, B. parapertussis was distinct from all other species (in

127 B. parapertussis was more similar to B. bronchiseptica t

128 erestingly, an O antigen-deficient strain of B. parapertussis was not defective in colonizing mice la

129 B. parapertussis was not detected in any specimens.

130 swabs from conventionally reared sheep, and B. parapertussis was recovered from 31.5% of the samples

131 In the case of B. parapertussis, which normally does not synthesize an

132 The persistence of Bordetella pertussis and B. parapertussis within vaccinated populations and the r

133 from a range of bacteria, is altered in the B. parapertussis WlbH protein.