ライフサイエンスコーパス: B. quintana

1 B. quintana has adapted to both the human host and body

2 B. quintana has the highest known hemin requirement amon

3 inst antigens of B. henselae (titer, 1,024), B. quintana (titer, 128), and the feline isolate (titer,

4 dimensional immunoblotting with sera from 21 B. quintana-infected patients to identify 24 consistentl

5 Second, a B. quintana strain hyperexpressing Irr was constructed;

6 Bartonella henselae and B. quintana induce an unusual vascular proliferative tis

7 onsible for human infection, B. henselae and B. quintana, cause prolonged febrile illness in immunoco

8 Two species, B. henselae and B. quintana, have been associated with bacillary angioma

9 B. henselae and B. quintana, the organisms that cause bacillary angiomat

10 munohistochemistry ruled out B. henselae and B. quintana.

11 r, endocarditis, and bacillary angiomatosis, B. quintana must survive and replicate in the disparate

12 lysis of global transcriptional responses by B. quintana.

13 hemin binding proteins (Hbps) synthesized by B. quintana that bind hemin on the outer surface but sha

14 During the infectious cycle, B. quintana transitions from the hemin-restricted human

15 Each B. quintana Vomp appears to contribute a different adhes

16 To estimate the prevalence of past exposure B. quintana among this population, a serosurvey was cond

17 ecently developed the first animal model for B. quintana infection, and using this model, we demonstr

18 endocarditis, 2, 4, and 2 were positive for B. quintana, B. henselae, and C. burnetii, respectively,

19 ial virulence determinant characterized from B. quintana.

20 that eight membrane-associated proteins from B. quintana bind hemin and that a approximately 25-kDa p

21 artonella quintana antigen, though sera from B. quintana-infected patients did react to Bh83.

22 B. henselae, B. quintana, and C. burnetii seropositivity was not stro

23 species and subspecies: Bartonella henselae, B. quintana, B. washoensis, and B. vinsonii subsp. berkh

24 re not reactive against Bartonella henselae, B. quintana, or B. elizabethae antigens but were serorea

25 y 24 consistently recognized, immunoreactive B. quintana antigens that have potential relevance for p

26 an hbpA promoter-lacZ reporter construct in B. quintana demonstrates that a transcriptional regulato

27 kerless deletion of the entire vomp locus in B. quintana.

28 Intact B. quintana treated with purified anti-HbpA Fab fragment

29 and growth phase was the induction of known B. quintana virulence genes and several previously unann

30 de that RpoE has a role in the adaptation of B. quintana to the hemin-rich arthropod vector environme

31 The genomic context of B. quintana rpoE identified it as a member of the ECF15

32 Additionally, growth of B. quintana was significantly enhanced by the presence o

33 We isolated the total membrane proteins of B. quintana and identified 60 proteins by two-dimensiona

34 The DeltarpoE DeltanepR mutant strain of B. quintana established that RpoE-mediated transcription

35 ase, will facilitate pathogenesis studies of B. quintana.

36 to define the outer membrane subproteome of B. quintana in order to obtain insight into the biology

37 n is important in mediating the tolerance of B. quintana to high hemin concentrations.

38 ression during infection with B. henselae or B. quintana.

39 rvation during infection with B. henselae or B. quintana.

40 ing pathogen and to identify the predominant B. quintana antigens targeted by the human immune system

41 prominent among the differentially-regulated B. quintana genes.

42 In this study, we examined the B. quintana RpoE response to two stressors that are enco

43 o experiments provide novel insight into the B. quintana transcriptional program within the body lous

44 d that transcription of 7% (93 genes) of the B. quintana genome is modified in response to change in

45 ox." Fourth, we used the H-box to search the B. quintana genome and discovered a number of intriguing

46 We sought to determine whether the B. quintana Vomp adhesins are necessary for infection in

47 13.6%; to Bartonella elizabethae, 12.5%; to B. quintana, 9.5%; to B. henselae, 3.5%; to Seoul virus,

48 Microimmunofluorescent titers to B. quintana in 192 clinic patients were compared with ti

49 we hypothesized that a previously unstudied B. quintana ECF sigma factor, RpoE, is involved in the t

50 ttern within the hbp family is revealed when B. quintana is grown in a range of hemin concentrations:

51 rpoE expression also was upregulated when B. quintana was exposed to high hemin concentrations.

52 c bone lesions were strongly associated with B. quintana, whereas peliosis hepatis was associated exc

53 er-city Seattle population was infected with B. quintana.

54 ox element previously shown to interact with B. quintana Irr.

55 Of the 39 patients with B. quintana titers > or = 64, 24 (62%) also had titers >

56 ed with B. henselae and 23 (47 percent) with B. quintana.

57 exposure (P< or =0.004), whereas those with B. quintana were clustered and were characterized by low