ライフサイエンスコーパス: BAC

1 BAC biotransformation rates of the enriched microbial co

2 BAC cloning and expression analyses were employed to cha

3 BAC contigs encompass a 1.5-Mb genomic region with 1 Mb

4 BAC fxAR121 mice develop systemic and neuromuscular phen

5 BAC recombineering, followed by Tol2 transgenesis, was u

6 BAC transgenic mammalian systems offer an important plat

7 BAC transgenic mice expressing mHTT lacking the N17 doma

8 In this study, we sequenced 13 BAC clones derived from LCR22A/D and aligned them with 1

9 A 3D BAC pool set generated in this study was screened using

10 Using a cocktail of 41 BACs in three colors, all chromosome pairs could be indi

11 sical map, and assembled sequences from 4355 BACs.

12 solution, we identified and sequenced 15 622 BACs representing the minimal tiling path of 72 052 phys

13 sequencing and with short reads from 31,719 BAC clones, thereby achieving phased blocks with an N50

14 ssembly and paired-end sequences from 62,758 BAC clones provides strong support for the robustness of

15 A Spirodela cytogenetic map containing 96 BAC markers with an average distance of 0.89 Mbp was con

16 e the contribution of muscle, we developed a BAC mouse model featuring a floxed first exon to permit

17 lidate these results in vivo by generating a BAC transgenic mouse, which overexpresses Cyfip1 under t

18 engineered a transgenic mouse that harbors a BAC with an authentic, mutated, splice-defective human B

19 ckdown of eRNAs, and the deletion of L2 in a BAC transfection assay.

20 ble to efficiently modify two positions of a BAC simultaneously by co-transformation of a single-stra

21 ystem has been designed and constructed on a BAC, its implementation and optimization in a recipient

22 er group of ten predictors still providing a BAC of 71.7% in 108 patients never used for model discov

23 We now report a BAC mouse model of C9orf72 ALS/FTD that shows decreased

24 Here we have used a BAC transgenic mouse line, alpha5(GFP), to examine the c

25 We used a BAC-by-BAC approach to sequence the MSY and resequence t

26 Here, using a BAC-CreERT-dependent genetic lineage-tracing strategy, w

27 lly, we mutated the human mHTT gene within a BAC to express either an aspartic acid or an alanine at

28 oss-validation (test-fold balanced accuracy [BAC] of 75.0% for 4-week outcomes and 73.8% for and 52-w

29 lity in Barrett's esophageal adenocarcinoma (BAC).

30 (ESCC, n=49) and Barrett's adenocarcinomas (BAC, n=61) revealed similar STAT3 expression in ESCCs an

31 elomere attrition and apoptotic death in all BAC cell lines tested, relative to either treatment alon

32 0-1.0 mg O3/mg dissolved organic carbon) and BAC empty bed contact times (EBCT; 15-60 min) on the for

33 l STAT3-regulated genes involved in ESCC and BAC cell proliferation and cell migration were identifie

34 kdown reduces cell proliferation in ESCC and BAC cells, inhibits migration of BAC cells and may suppo

35 ytene chromosomes, clone-based finishing and BAC fingerprint verification, ordering of scaffolds by a

36 hage-derived artificial chromosome (PAC) and BAC recombinants.

37 d the recombinase-mediated BAC targeting and BAC recombineering techniques to dissect the functions o

38 a mixture of dextrin/peptone and BACs), and BACs-enriched (B, fed only BACs).

39 vealed similar STAT3 expression in ESCCs and BACs (P=0.109), but preferentially activated P-STAT3 in

40 STAT3 expression and activation in ESCCs and BACs.

41 d (DPB, fed a mixture of dextrin/peptone and BACs), and BACs-enriched (B, fed only BACs).

42 We have generated, sequenced and annotated BAC sequences spanning the S locus, and identified its c

43 ery well described by the band anticrossing (BAC) model in which localized nitrogen states interact w

44 n to assembly-independent resources, such as BAC clone end sequences and PacBio long reads, indicate

45 We sequenced and assembled BAC clones corresponding to a 1.3-Mbp complex region of

46 tion, using a whole-genome shotgun assembly, BAC physical mapping, and clone-based finishing.

47 nd aligned them with 15 previously available BAC sequences to create a new genetic variation map.

48 the development of a set of universal avian BAC clones that permit rapid anchoring of multiple scaff

49 L96 C-terminal alanine conversion mutant (B6-BAC) virus was serially passaged in cell culture, it gai

50 L96 coding region was engineered into the B6-BAC virus, it significantly increased the plaque size an

51 Introducing the B6.BAC into the NOD background, which is genetically defici

52 Experimental results on over 15 000 barley BACs and over 4000 cowpea BACs demonstrate a significant

53 path of 72 052 physical-mapped gene-bearing BACs.

54 In total, 52,270 BESs (BAC-end sequences), equivalent to 32 Mbp (~6 %) of the g

55 recapitulating expression of marker genes by BAC transgenesis or knock-in has generated useful transg

56 This EB phenotype was fully rescued by BAC or cDNA complementation but not by the reduction of

57 We used a BAC-by-BAC approach to sequence the MSY and resequence the Y re

58 The C9ORF72 BAC transgenic mice will be a valuable tool in the study

59 neration method for beaded activated carbon (BAC) loaded with n-dodecane, a high molecular weight vol

60 VOCs mixtures onto beaded activated carbon (BAC).

61 Beaded activated carbon (BAC, microwave-absorbing) and a polymeric adsorbent (V50

62 ncapsulation of bovine adrenocortical cells (BACs) in alginate (enBACs).

63 s limitation, we generated and characterized BAC transgenic P2rx4 tdTomato reporter mice.

64 In chimeric BACs, the mTert promoter became strongly repressed in th

65 ESC lines with chimeric BACs, in which two TERT promoters were swapped, were als

66 that they were found in human and chimpanzee BAC and FOSMID clones sequenced as part of the original

67 Benzalkonium chlorides (BACs) are emerging pollutants.

68 The effect of benzalkonium chlorides (BACs), a widely used class of quaternary ammonium disinf

69 biotransformation of benzalkonium chlorides (BACs)-an active ingredient of many disinfectants-to benz

70 Using these chromatinized BACs, we showed that hTERT silencing during differentiat

71 ructed on a bacterial artificial chromosome (BAC) by using a recombineering-based inchworm extension

72 iotinylated bacterial artificial chromosome (BAC) capture and multiplexed pooled capture for SNP/INDE

73 infectious bacterial artificial chromosome (BAC) clone.

74 ng reads to bacterial artificial chromosome (BAC) clones (in the context of the combinatorial pooling

75 cted potato bacterial artificial chromosome (BAC) clones in a set of 16 potato cultivars with diverse

76 uence eight Bacterial Artificial Chromosome (BAC) clones spanning the horse MHC class II region.

77 from mouse bacterial artificial chromosome (BAC) constructs closely mimics endogenous LRRK2 distribu

78 ibe two VZV bacterial artificial chromosome (BAC) constructs with ORF54 gene deletions, Delta54L (ful

79 carrying a bacterial artificial chromosome (BAC) containing the full human C9orf72 gene with either

80 nstructed a bacterial artificial chromosome (BAC) contig, and obtained a continuous genomic sequence

81 uences from bacterial artificial chromosome (BAC) inserts and contigs derived from a low coverage nex

82 Two bacterial artificial chromosome (BAC) libraries of a homozygous doubled haploid plant of

83 assembly, a bacterial artificial chromosome (BAC) physical map, and assembled sequences from 4355 BAC

84 -1:100,000) bacterial artificial chromosome (BAC) recombinants require selectable markers.

85 Using Bacteria Artificial Chromosome (BAC) recombineering and a transgenic knock-in, we have g

86 trategy for Bacterial Artificial Chromosome (BAC) recombineering based on co-selection is described.

87 constructed bacterial artificial chromosome (BAC) reporters using human and mouse genomic DNAs encomp

88 single-copy bacterial artificial chromosome (BAC) reporters, covering hTERT and mTERT genes and their

89 reads, and bacterial artificial chromosome (BAC) sequencing approaches.

90 gy based on bacterial artificial chromosome (BAC) technology.

91 n vivo DRD1-Bacterial Artificial Chromosome (BAC) Tet-on system allowing for the inducible activation

92 pathogenic bacterial artificial chromosome (BAC) that is used to study MCF.

93 ws a single Bacterial Artificial Chromosome (BAC) transgene to direct sparse labeling of genetically-

94 ing a human bacterial artificial chromosome (BAC) transgene were generated, resulting in plasma level

95 Using bacterial artificial chromosome (BAC) transgenic HeLa and mouse embryonic stem cells stab

96 s have used bacterial artificial chromosome (BAC) transgenic mice expressing channelrhodopsin-2 (ChR2

97 ng HDC-EGFP bacterial artificial chromosome (BAC) transgenic mice in which EGFP expression is control

98 creating a bacterial artificial chromosome (BAC) transgenic model that recapitulates the inv(3)(q21;

99 in a single bacterial artificial chromosome (BAC) vector containing the mouse HoxB cluster.

100 a number of bacterial artificial chromosome (BAC) were sequenced, annotated and the presence of repet

101 igh-quality bacterial artificial chromosome (BAC)-based assemblies to evaluate base-level accuracy.

102 reported a bacterial artificial chromosome (BAC)-based lymphatic reporter mouse, where EGFP is expre

103 y developed bacterial artificial chromosome (BAC)-based MHV reverse genetics system.

104 uses in the bacterial artificial chromosome (BAC)-derived clinical HCMV strain TB40/E-mCherry.

105 n by either bacterial artificial chromosome (BAC)-derived virus in Jjhan cells or wild-type HHV-6A st

106 tgun reads, bacterial artificial chromosome (BAC)-end sequences and genotype-by-sequencing genetic ma

107 unction via bacterial artificial chromosome (BAC)-mediated overexpression of the vesicular monoamine

108 y the B95-8 bacterial artificial chromosome (BAC).IMPORTANCE Epstein-Barr virus (EBV) infects the maj

109 e only 6278 bacterial artificial chromosome (BACs) in the physical map were sequenced, fine structure

110 cloned as a bacterial artificial chromosome [BAC]) has a 4-bp deletion that disrupts GP129, which enc

111 quences in bacterial artificial chromosomes (BAC) to analyze the genomic region surrounding the Eya1

112 lly mapped bacterial artificial chromosomes (BACs) containing Spirodela DNA inserts with little or no

113 to deliver bacterial artificial chromosomes (BACs) into cells by viral transduction.

114 Bacterial artificial chromosomes (BACs) provide a stable, genetically defined source of vi

115 study, VZV bacterial artificial chromosomes (BACs) were generated with small (Delta30S), medium (Delt

116 Finally, bacterial artificial chromosomes (BACs) were isolated that contained the three loci.

117 oning into bacterial artificial chromosomes (BACs), and then virus is regenerated by DNA transfection

118 mbineering bacterial artificial chromosomes (BACs), it is common practice to design the ends of the d

119 mids up to bacterial artificial chromosomes (BACs; 144 kb deletion) have been achieved by this method

120 a more efficient approach than the classical BAC transgenesis, resulting in complete BAC integration

121 d SeqCNV to both bacterial artificial clone (BAC) and human patient NGS data to identify CNVs.

122 d using three aerobic microbial communities: BACs-unexposed (DP, fed a mixture of dextrin/peptone), B

123 ese may be particularly useful for comparing BAC-based physical maps with genetic maps.

124 ical BAC transgenesis, resulting in complete BAC integration with predictable end sequences, thereby

125 pruritus and serum bile acid concentration (BAC) as well as an improvement of serum liver tests.

126 relation to the blood alcohol concentration (BAC) as a function of gender.

127 e resulting in blood alcohol concentrations (BACs) >/=80 mg% within a 2-h period) is the most prevale

128 nt protein-modified Nodal BACs and confirmed BAC functionality by generating fluorescent reporter mic

129 reathalyzers estimate Blood Alcohol Content (BAC) from the concentration of ethanol in the breath.

130 apture and hybridization to large contiguous BAC baits reduces sample and probe hybridization variabi

131 over 15 000 barley BACs and over 4000 cowpea BACs demonstrate a significant improvement in the qualit

132 full rescue with ubiquitous Cre, we crossed BAC fxAR121 mice with Human Skeletal Actin-Cre mice.

133 d rapid capture strategies using the current BAC set provide the basis for assembling numerous avian

134 of bis(diisopropylamino)cyclopropenylidene (BAC) to provide PhB((i)Pr2Im)3Fe(CN)(N2)(BAC).

135 egradation of a N,N'-bis(acryloyl)cystamine (BAC) cross-linked core out of a non-degradable pNIPAM sh

136 cept, we generated D1-dopamine receptor (D1) BAC MORF mice that label about 1% striatal D1-expressing

137 Four microbial communities degrading BACs were developed from sewage (SEW), activated sludge

138 rize a UL93 stop mutant virus (UL93st-TB40/E-BAC) to demonstrate that the absence of this protein doe

139 e the species mismatch, the mouse Prox1-EGFP BAC enabled a reliable expression of EGFP in Prox1-expre

140 atic reporter rat using the mouse Prox1-EGFP BAC.

141 Finally, we show that Sox8-EGFP BAC mice can be used to interrogate the altered dSPN dev

142 ive expression of EGFP in dSPNs of Sox8-EGFP BAC mice is maintained at postnatal timepoints.

143 marker of embryonic dSPNs and the Sox8-EGFP BAC transgenic mice are an excellent tool to study the d

144 Sox8 in the embryonic striatum and Sox8-EGFP BAC transgenic mice specifically reveal the direct pathw

145 hromosomes contain most of the gene-enriched BACs and are characterized by high recombination rates,

146 vitroby deriving virus from a self-excising BAC in fibroblasts and repressing RL13 and UL128L.

147 ty, we engineered a B6.CTLA-4 (floxed-Exon2)-BAC-transgene, resulting in selective expression of li-C

148 We subsequently incorporated 382 finished BAC clone sequences to generate a draft assembly, CHM1_1

149 ld paired-end sequences assisted by fivefold BAC-to-BAC sequences and a high-resolution genetic map.

150 uses derived from TR-BAC, TB40-BAC4, and FIX-BAC in either fibroblast or epithelial cells was associa

151 nucleotides in UL128L from TB40-BAC4 or FIX-BAC.

152 ing UL128L nucleotides from TB40-BAC4 or FIX-BAC.

153 Thus, an efficient method for BAC transgenesis in rats would be valuable.

154 a full copy of the gene, generating the {for(BAC)} rescue allele.

155 Genomes of four BAC degrading and nondegrading BIOMIG1 phenotypes were s

156 neurite shortening in neuronal cultures from BAC transgenic R1441G-LRRK2 mice.

157 sion, neurite length of primary neurons from BAC transgenic G2019S-LRRK2 mice returned back to wild-t

158 reaction involves carbon atom transfer from BAC to create a cyanide ligand along with the alkyne (i)

159 n E. coli resulted in formation of BDMA from BACs at a rate of 14 muM h(-1).

160 Viruses were generated from BACs containing the genomes of strains TR, TB40, FIX, an

161 nsgenic GFP expression directed by the Gata1-BAC faithfully recapitulated the endogenous Gata1 expres

162 P2X4 receptor-expressing cells, we generated BAC transgenic mice expressing tdTomato under the contro

163 eutic strategies, difficulties of generating BAC transgenic rats have hindered progress.

164 Here we provide characterization of GENSAT BAC-Cre driver lines with expression in specific neuroan

165 ostnatal day 0 (PD0) Drd1a-tdTomato/Drd2-GFP BAC transgenic mice, and at the receptor level by costai

166 ely dissociated from two lines of Phox2b-GFP BAC transgenic mice.

167 el used, mice carry the Cited1-CreER(TM)-GFP BAC transgene in which a tamoxifen-inducible Cre (CreER(

168 o test for Ag receptor signaling, Nur77(gfp) BAC transgenic mice, which upregulate GFP in response to

169 heral targeting regions (PTRs) within an HBB BAC bias a competition between pericentric versus periph

170 Here we generate a series of hGH/BAC transgenes specifically modified to identify structu

171 in the neural tube when inserted into a HoxA BAC reporter.

172 A human BAC clone containing the G72/G30 genomic region was used

173 , LRRK2-G2019S expression derived from human BAC constructs causes LRRK2 to be expressed in additiona

174 The distribution of LRRK2 from human BAC constructs more closely resembles descriptions of LR

175 ine increased the production of ACh in human BACs, and ACh acts as a growth factor for these cells.

176 Vesamicol induced potent apoptosis of human BACs in cell culture and nude mice models.

177 romosome fluorescence in situ hybridization (BAC-FISH).

178 In BAC transgenic zebrafish, cav1 regulatory sequences drov

179 s such as vesamicol may have applications in BAC therapy.

180 on of a narrow intermediate band (E- band in BAC model) with the minimum at the Gamma point of the Br

181 t both tumor growth and genomic evolution in BAC.

182 Bile analyses showed an increase in BAC with 4-PB.

183 We demonstrate that telomerase inhibition in BAC cells increases HR activity, RAD51 expression, and a

184 n combination with telomerase inhibition, in BAC.

185 l as markedly reduced genomic instability in BAC cells over time.

186 than those reported to date, are involved in BAC biotransformation in various habitats.

187 integrase and a dioxygenase were involved in BAC biotransformation.

188 ntrast, about 90% Treg depletion obtained in BAC transgenic Foxp3.LuciDTR4 mice failed to induce comp

189 ks after the onset of the motor phenotype in BAC-Q72 mice.

190 assist in their maintenance/stabilization in BAC cells.

191 is potential linkage, the STN was studied in BAC transgenic and Q175 knock-in mouse models of HD.

192 Neither CRISPR/Cas9 nor TALEN increased BAC transgenesis.

193 including a set of end-sequenced and indexed BAC clones and 100x Illumina whole-genome shotgun (WGS)

194 nerated tissue-specific cell cycle indicator BAC transgenic mouse lines.

195 The infectious BAC (iBAC) library contains 184,320 clones with an avera

196 Previously, expensive and labor-intensive BAC-based techniques were used to sequence the Y for a h

197 enerated conditional Zic3 mice and Zic3-LacZ-BAC reporter mice.

198 Transgenic mice harboring a linked BAC developed leukemia accompanied by EVI1 overexpressio

199 study, we employed the recombinase-mediated BAC targeting and BAC recombineering techniques to disse

200 r neighboring loci, via recombinase-mediated BAC targeting.

201 Viruses derived from a Merlin-BAC derivative in which RL13 and UL128L were either muta

202 This was not observed for Merlin-BAC, from which the vector is excised in derived viruses

203 Viruses derived from Merlin-BAC in fibroblasts had mutations in UL128L, but mutation

204 B40, FIX, and Merlin, as well as from Merlin-BAC recombinants containing variant nucleotides in UL128

205 sent in various environments and mineralizes BACs.

206 The strain BIOMIG1 mineralizes BACs at a rate up to 2.40 mumol hr(-1) 10(-11) cells.

207 his end, we generated a novel multicistronic BAC (bacterial artificial chromosome) transgenic mouse l

208 ne (BAC) to provide PhB((i)Pr2Im)3Fe(CN)(N2)(BAC).

209 om communities after prolonged periods of no-BAC exposure, suggesting that they are robust BAC-degrad

210 seamless fluorescent protein-modified Nodal BACs and confirmed BAC functionality by generating fluor

211 A novel BAC transgenic Neurog3 reporter detected two types of mi

212 imilar between the two wastewaters across O3/BAC conditions.

213 followed by biological activated carbon (O3/BAC) is being considered as a key component of reverse o

214 Using a laboratory-scale O3/BAC system treating two nitrified wastewater effluents,

215 calculated DBP-associated toxicity of the O3/BAC-treated chloraminated effluents were comparable or s

216 The O3/BAC-treated wastewaters met regulatory levels for trihal

217 mulation in OE21 (ESCC) cells, whereas OE33 (BAC) cells showed constitutive weak STAT3 activation.

218 Analysis of BAC transgenic mouse strains harboring an engineered del

219 and (ii) the problem of de novo assembly of BAC clones.

220 The generation and characterization of BAC-transgenic eGFP reporter mouse lines has revealed th

221 Here we report the development of BAC libraries, sub genome specific physical maps, and a

222 , two laboratories report the development of BAC transgenic mice that recapitulate features of the hu

223 us accumbens, thalamus, and hypothalamus) of BAC aldh1l1-translational ribosome affinity purification

224 the anchoring of 100 Mb of WGS and 420 Mb of BAC sequences, an exploration of genetic diversity along

225 in ESCC and BAC cells, inhibits migration of BAC cells and may support cell migration of ESCC cells.

226 ted genome assembly; ii) demonstrated NGS of BAC pools as a potential approach for mining candidates

227 vector to only two steps: a single round of BAC modification followed by a retrieval step.

228 We then used a second round of BAC recombineering to create deletion constructs of thes

229 ft of the unstable repeat DNA in a subset of BAC-expressing neurons results in the in-frame translati

230 In addition, the utility of BAC constructs Delta54L and Delta54S as tools for the is

231 the genes involved in the biodegradation of BACs is crucial for better understanding the fate of BAC

232 crucial for better understanding the fate of BACs in the environment and developing treatment strateg

233 that BIOMIG1 plays a key role on the fate of BACs in the environment and genes, other than those repo

234 ave applications for controlling the fate of BACs in the environment.

235 ate significantly increased the life span of BACs.

236 ne and BACs), and BACs-enriched (B, fed only BACs).

237 u hybridization (mcFISH), using an optimized BAC pooling strategy, to validate its physical map and c

238 to LCR22A, using the variation map from our BAC analysis to help resolve allele ambiguity, and by pe

239 regions were possible because of overlapping BAC clones, generated by an expensive and labor-intensiv

240 ng 82 S-linked genes anchored to overlapping BACs.

241 osed (DP, fed a mixture of dextrin/peptone), BACs-exposed (DPB, fed a mixture of dextrin/peptone and

242 Using a Pkd1-BAC recombineering approach, we developed murine models

243 The ingestion of cachaca by women produced BAC levels significantly smaller than those obtained for

244 A total of 134,827 high quality BAC-end sequences (BESs) were generated from the 73,728

245 R-BAC is well tolerated and active against MCL.

246 tween LRRK2 and tau, we crossed LRRK2 R1441G BAC transgenic mice (Mus musculus) with tau P301S mutant

247 f ZsG by mice transgenic for the recombinant BAC appears to be a faithful surrogate for TSLP expressi

248 Two groups recently reported BAC mouse models that produce RNA foci and RAN proteins

249 AC exposure, suggesting that they are robust BAC-degraders.

250 For seamless BAC mutagenesis, selectable markers need to be removed a

251 lectable markers, allowing one-step seamless BAC mutagenesis.

252 The use of co-selection BAC recombineering reduces the DNA manipulation needed t

253 esource for anchoring and ordering sequenced BAC and next generation sequencing (NGS) contigs.

254 useful for anchoring and ordering sequenced BAC and NGS based contigs for assembling a high-quality,

255 Exploration of these sequenced BACs revealed that although distal ends of chromosomes c

256 embly of the pig Y Chromosome, by sequencing BAC and fosmid clones from Duroc animals and incorporati

257 ve generated for the first time, human SIRPA BAC transgenic rats, for which the gene is faithfully ex

258 To do this, human SIRPA BAC was modified with elements to work in coordination w

259 ite-out validation across 44 European sites (BAC of 72.1% for 4-week outcomes and 71.1% for 52-week o

260 the large CNVs associated with two specific BACs (RH102I10 and RH83C08) were widespread among potato

261 information was used for anchoring sugarcane BAC clones to the sorghum genome sequence.

262 Here we analyzed Drd1a-tdTomato BAC transgenic mice and found that the dopamine D1 recep

263 The BAC interaction results in the formation of a narrow int

264 The BAC map was partitioned by sub genomes through alignment

265 The BAC transgene drives cell-type-specific transcription of

266 m that the deconvolution is accurate and the BAC assemblies have good quality.

267 The splitting of the conduction band by the BAC interaction is further confirmed by a direct observa

268 n of two RAREs, DE-RARE and ENE-RARE, in the BAC completely abolished the rostral expansion of the 5

269 ASO7 had a similar effect in the BAC-Q72 SCA2 mouse model, and in both mouse models it no

270 Here, sequencing of the BAC clone showed that the long unique region (LUR) of th

271 ered the physical/chemical properties of the BAC.

272 Overall, these results indicate that the BAC-based MHV reverse genetics system will be useful for

273 e previously sequenced strain from which the BAC was derived, and identified the duplication and tran

274 Sec23b pancreatic function reside within the BAC transgenes.

275 These BAC sequences constitute a resource to improve the effic

276 ering to create deletion constructs of these BAC reporters to localize enhancer activity more precise

277 Three BAC libraries were constructed, fingerprinted, and integ

278 tegrated with wearable devices for real-time BAC monitoring.

279 are HarvEST:Barley provides facile access to BAC sequences and their annotations, along with the barl

280 detects ethanol in the breath equivalent to BAC from 0.01% to 0.2%.

281 can occur not only during isolation prior to BAC cloning but also when virus is regenerated.

282 ed-end sequences assisted by fivefold BAC-to-BAC sequences and a high-resolution genetic map.

283 Towne bacterial artificial chromosome (Towne-BAC) genome, replacing the conserved negatively charged

284 ed a plaque size comparable to that of Towne-BAC, displayed an altered restriction fragment length pa

285 s (UL96P10) and the similarly passaged Towne-BAC virus revealed major differences only in the RNA4.9

286 Propagation of viruses derived from TR-BAC, TB40-BAC4, and FIX-BAC in either fibroblast or epit

287 trast to plasmid-based reporters, transgenic BAC reporters faithfully recapitulated endogenous gene e

288 reater that approximately 0.4 Mb between two BAC clone markers that must contain D.

289 We also demonstrate that two BAC transgenes spanning Sec23b rescue the lethality of m

290 We used BAC recombineering to first create GFP reporter construc

291 Using BAC transgenesis, we created a mouse model with a humani

292 Using BAC-Cre transgenic mice with viral tracing techniques, w

293 mouse models expressing these mutants using BAC recombineering technology and investigated their abi

294 bsequent restoration of pathogenicity, using BAC-based mutagenesis.

295 Utilizing BAC transgenesis, we generated transgenic zebrafish in w

296 We have identified conditions under which BAC-derived viruses containing an intact, wild-type geno

297 epresents a new member of RO associated with BAC degradation, and have applications for controlling t

298 structed, fingerprinted, and integrated with BAC-end sequences (BES) to produce a de novo whole-genom

299 ation generally decreased significantly with BAC treatment at 15 min EBCT, but little further reducti

300 We found that, compared with BACs in monolayer culture, encapsulation in alginate sig