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1 teremia was always significantly shorter for BACTEC.
4 ained smears were prepared from 666 positive BACTEC 12B bottles and examined for the presence or abse
6 Mycobacterium tuberculosis complex (MTBC) in BACTEC 12B broth cultures of respiratory specimens was e
7 bacterium tuberculosis complex (MTBC) in 125 BACTEC 12B broth cultures with positive growth indices.
10 used to detect Mycobacterium tuberculosis in BACTEC 12B medium cultures when they first gave a growth
11 he same tissues tested by PCR using modified BACTEC 12B medium failed to grow M. avium subsp. paratub
12 luation of the presence of cord formation in BACTEC 12B medium is reliable and permits the rapid pres
15 sing a common lot of microdilution trays and BACTEC 12B medium, pH 6.8; strains were tested once on t
25 O/F LYTIC for 33 of 340 pairs, 14.1 days for BACTEC 13A versus 11.6 days for BacT/ALERT MB for 38 of
26 ve adequately paired sets were 15.3 days for BACTEC 13A versus 12.8 days for MYCO/F LYTIC for 33 of 3
27 /ALERT MB for 38 of 380 pairs, 12.6 days for BACTEC 13A versus 20.0 days for ISOLATOR 10 for 26 of 26
28 +/- 1 ml) sets from which MAC was recovered, BACTEC 13A was positive for 19 (73%), BACTEC MYCO/F LYTI
30 ts were compared to those obtained using the BACTEC 460 (BD, Sparks, MD) radiometric method and a bro
31 an time until detection of 10.1 days for the BACTEC 460 and 14.2 days for the MB/BacT (P = 0.009).
32 levofloxacin concentrations of 2 microg/ml (BACTEC 460 and BACTEC MGIT 960) and 1 microg/ml (AP) inh
33 levofloxacin in the two newer test systems (BACTEC 460 and BACTEC MGIT 960), media containing subinh
37 utum was collected for quantitative culture, Bactec 460 liquid culture, and mRNA analysis throughout
38 In comparison with the previously described BACTEC 460 M. paratuberculosis counting method, quantifi
40 and ethambutol were compared to those of the BACTEC 460 method, and discrepant results were checked b
44 spite the documented enhanced ability of the BACTEC 460 radiometric mycobacterial culture system to r
45 hod, agar proportion (AP), the commonly used BACTEC 460 radiometric system, and the newer BACTEC MGIT
48 ibiotic supplement kit was compared with the BACTEC 460 system (BACTEC 460) in a test of 488 specimen
51 ed by MABA and the results obtained with the BACTEC 460 system were 87.9% for initial results and 93.
53 ed with the BACTEC MGIT 960, followed by the BACTEC 460 TB (271, or 75%) and solid media (250, or 69%
54 ional solid media; the number recovered with BACTEC 460 TB plus solid media was 128 (97%), that recov
57 were inoculated into the BACTEC MGIT 960 and BACTEC 460 TB systems, as well as onto Lowenstein-Jensen
58 lid media was 121 (92%), that recovered with BACTEC 460 TB was 119 (90%) and that recovered with all
59 147 (85%) for BACTEC MGIT 960, 123 (72%) for BACTEC 460 TB, and 106 (62%) for all solid media combine
60 tems were 8.1% for BACTEC MGIT 960, 4.9% for BACTEC 460 TB, and 21.1% for all solid media combined.
63 tified by both systems was 11.9 days for the BACTEC 460 versus 13.7 days for the MB/BacT (P = 0.046).
65 kit was compared with the BACTEC 460 system (BACTEC 460) in a test of 488 specimens submitted for myc
67 (n = 88), and concordance values between the Bactec 460, the Wayne test, or pncA gene Sanger sequenci
72 weeks of incubation was only 2.8 or 8.4% in BACTEC-460 (for a GI of 10 or 500) but 17.7% in MB Redox
73 e evaluated in comparison to the radiometric BACTEC-460 semiautomated system for recovery of Mycobact
74 ulture-positive specimens, occurred with the BACTEC-460 system (92.2%), followed by the MB Redox tube
75 spite these differences in comparison to the BACTEC-460 system and some differences between the MGIT
76 ems presents a reasonable alternative to the BACTEC-460 system, especially for laboratories with a li
77 tion was observed also among the cultures in BACTEC-460: a mean of 12 days to a growth index (GI) of
79 es determined to be resistant to EMB by both BACTEC 460TB and AP methods were almost always resistant
80 eakpoints, was 100% for all strains with the BACTEC 460TB method (both drugs and both pH values) and
81 s could not be confirmed as resistant by the BACTEC 460TB method or by repeat testing with the AP met
83 that the AP method is more accurate than the BACTEC 460TB method, laboratories should not report EMB
84 trains, borderline results obtained with the BACTEC 460TB method, the presence of microcolonies deter
88 pared with those obtained with the reference BACTEC 460TB system combined with standard DNA sequencin
90 was 100% for all strains and both drugs when BACTEC 460TB was used, regardless of the pH of the mediu
93 bottles had no growth detected; 50 from the Bactec (5 aerobic and 45 anaerobic) and 15 from the BacT
95 ten with BACTEC 9240 (58 cultures) than with BACTEC 9050 (43 cultures), but false-negative cultures w
98 of organisms and time to detection with the BACTEC 9050 and BACTEC 9240 systems were compared in a m
101 , more false-negative cultures occurred with BACTEC 9240 (11 cultures) than with BACTEC 9050 (8 cultu
102 3) more false-positive signals occurred with BACTEC 9240 (15 cultures) than with BACTEC 9050 (4 cultu
103 which were recovered earlier (P < 0.01) with BACTEC 9240 (35.0 h) than with BACTEC 9050 (61.4 h).
104 se-positive signals occurred more often with BACTEC 9240 (58 cultures) than with BACTEC 9050 (43 cult
106 RT (BioMerieux, Inc., Durham, N.C.) (BM) and BACTEC 9240 (BD Diagnostic Systems, Sparks, Md.) (BD) au
108 In a previous study which evaluated the BACTEC 9240 automated blood culture system (Becton Dicki
112 he reliability of MYCO/F Lytic medium in the BACTEC 9240 blood culture system was evaluated by compar
114 , a liquid medium developed for use with the BACTEC 9240 blood culture system, was compared to the Is
116 ity of the BACTEC Peds Plus/F bottle and the BACTEC 9240 instrument (Becton Dickinson Diagnostic Inst
117 were analyzed for bacterial growth using the BACTEC 9240 instrument and for the bacterial 16S rRNA ge
118 e combination of MYCO/F Lytic medium and the BACTEC 9240 instrument is an excellent blood culture sys
120 t instrument false positives signaled by the BACTEC 9240 system are not due to bacteria in the blood
122 incubation protocol was instituted with the BACTEC 9240 system for a 1-year period to determine the
124 d time to detection with the BACTEC 9050 and BACTEC 9240 systems were compared in a multicenter evalu
128 e extraction of a single tooth, with LyF and BACTEC aerobic cultures taking 78 and 30.5 h, respective
130 at a bottle (BACTEC Plus Aerobic/F bottle or BACTEC Anaerobic Lytic/10 bottle) culture was positive b
132 silosis (yellow fluorescence), was tested on Bactec and BacT/Alert blood culture bottles which signal
135 ee methods, agar proportion (AP), BACTEC460 (Bactec), and MGIT-960 (MGIT), yielded overall agreement
136 ement by methods was 91.3% for AP, 93.0% for Bactec, and 82.6% for MGIT and by drugs was 92.2% for IN
137 dia from three different manufacturers (ESP, BACTEC, and BacT/Alert) were included in the study.
138 ulture, acid-fast smear, days-to-positive by BACTEC, and Mycobacterium tuberculosis antigen 85 comple
140 assay was validated with DNA extracted from BACTEC blood culture bottles positive for yeasts (n = 62
141 samples taken directly from signal-positive Bactec blood culture bottles within 24 h of positive sig
142 f 131), respectively, with 200 GPCC-positive BACTEC blood culture bottles, and 100% (74 of 74) and 99
144 tex agglutination (PBP-LA) assay directly on Bactec blood culture broth samples containing Staphyloco
146 pared the MYCO/F Lytic bottle with two other BACTEC bottles and the Isolator system for the recovery
150 pole Laboratories, Cranbury, N.J.) and three BACTEC bottles: the MYCO/F Lytic bottle, the BACTEC Plus
151 remia was detected on 2.1% of occasions with BACTEC compared to 31% of occasions with LyF (P < 0.05)
152 identification of Staphylococcus aureus from BACTEC culture broth showed a sensitivity, a specificity
157 In bottles containing antifungal agents, Bactec FX recovered 83.1% of isolates, whereas VersaTREK
159 In the presence of antifungal agents, the Bactec FX recovery time was significantly faster than th
160 center trial, 155 blood cultures from the BD Bactec FX system and 212 from the bioMerieux BacT/Alert
161 vels of commonly used antifungal agents, the Bactec FX system demonstrated a significantly greater re
164 ere used, the BacT performed better than the Bactec in overall growth detection, time to growth detec
167 aliquot taken from BD BACTEC Plus and/or BD BACTEC Lytic/10 bottles (Becton Dickinson [BD], Sparks,
174 ver, none of the test methods, including the BACTEC method, accurately predicted the ampicillin resis
175 ting of Mycobacterium tuberculosis by the BD Bactec MGIT 320 using the BD Bactec MGIT 960 (BD Diagnos
176 Trek Diagnostics, Inc., Westlake, Ohio) and BACTEC MGIT 960 (BD Biosciences, Sparks, Md.)-were compa
177 losis by the BD Bactec MGIT 320 using the BD Bactec MGIT 960 (BD Diagnostics, Sparks, MD) as a refere
180 c Hain line probe assay (LPA) and phenotypic Bactec MGIT 960 analysis using 26 geographically diverse
181 Processed specimens were inoculated into the BACTEC MGIT 960 and BACTEC 460 TB systems, as well as on
182 ory routine procedure and were inoculated in Bactec MGIT 960 as well as Lowenstein-Jensen (LJ) medium
183 lse-positive signals occurred with 23 (0.7%) BACTEC MGIT 960 cultures and 84 (2.7%) ESP II cultures (
188 lid media was 128 (97%), that recovered with BACTEC MGIT 960 plus solid media was 121 (92%), that rec
189 boratory, instrument-negative tubes from the Bactec MGIT 960 system are inspected visually for clumps
191 mpared with the indirect method by using the Bactec MGIT 960 system in the context of patient screeni
192 e findings indicate that direct DST with the Bactec MGIT 960 system offers further time savings and i
193 cultured on Lowenstein Jensen media and the BACTEC MGIT 960 system, and identified using the Hain(R)
196 ecovery of all mycobacteria by ESP II and by BACTEC MGIT 960 were not significant; for the individual
197 oncentrations of 2 microg/ml (BACTEC 460 and BACTEC MGIT 960) and 1 microg/ml (AP) inhibited the grow
198 n the two newer test systems (BACTEC 460 and BACTEC MGIT 960), media containing subinhibitory levels
199 TD for MAC in each system were 10.0 days for BACTEC MGIT 960, 10.4 days for BACTEC 460 TB, and 25.9 d
200 re 172 (100%) for all systems, 147 (85%) for BACTEC MGIT 960, 123 (72%) for BACTEC 460 TB, and 106 (6
201 Overall contamination rates were 17.1% for BACTEC MGIT 960, 18.9% for ESP II, and 11.0% for Middleb
202 lates) for each of the systems were 8.1% for BACTEC MGIT 960, 4.9% for BACTEC 460 TB, and 21.1% for a
204 ntigen secreted by MTBC which is cultured in BACTEC MGIT 960, followed by the analysis of matrix-assi
205 of the 362 isolates) was recovered with the BACTEC MGIT 960, followed by the BACTEC 460 TB (271, or
212 r M. tuberculosis complex were 14.4 days for BACTEC MGIT, 15.2 days for BACTEC 460 TB, and 24.1 days
213 edium), 77 (42%) of 183 with liquid culture (Bactec MGIT960 system), and 174 (84%) of 207 with Xpert
215 BACTEC 13A (Becton Dickinson, Sparks, Md.), BACTEC MYCO/F LYTIC (Becton Dickinson), BacT/ALERT MB (b
217 d blood from patients in Malawi by using the BACTEC MYCO/F LYTIC (MFL), ISOLATOR 10 (Isolator), Septi
219 was shortest for BacT/ALERT MB, followed by BACTEC MYCO/F LYTIC and BACTEC 13A and then ISOLATOR 10.
221 the BacT/Alert MB system, that of the manual Bactec Myco/F Lytic procedure, and that of the Isolator
222 OLATOR 10 for 26 of 261 pairs, 12.8 days for BACTEC MYCO/F LYTIC versus 11.0 days for BacT/ALERT MB f
223 /ALERT MB for 33 of 340 pairs, 13.2 days for BACTEC MYCO/F LYTIC versus 20.4 days for ISOLATOR 10 for
224 23.8 days for Isolator 10, and 21.1 days for Bactec Myco/F Lytic versus 22.7 days for Isolator 10.
225 vered, BACTEC 13A was positive for 19 (73%), BACTEC MYCO/F LYTIC was positive for 21 (81%), BacT/ALER
226 State, including isolates and early positive Bactec mycobacterial growth indicator tube (MGIT) 960 cu
227 The specificities and sensitivities of the Bactec mycobacterial growth indicator tube (MGIT) system
229 bic medium versus that of the nonradiometric BACTEC NR-660 PEDS PLUS medium for the detection of seps
230 athogenic microorganisms were recovered from BACTEC NR6 (aerobic) bottles than from VITAL aerobic bot
232 on on Day 14 of therapy, days-to-positive in BACTEC on Day 30, and the baseline radiographic extent o
233 Lysis filtration (LyF) was compared with BACTEC PAEDS PLUS in estimating the prevalence of, and s
236 med on positive BacT/Alert Pediatric FAN and Bactec Peds Plus blood cultures with Gram-negative organ
237 y, TTD, and antibiotic neutralization in the Bactec Peds Plus system compared to those in the Pediatr
238 for the rapid identification of pathogens in Bactec Peds Plus/F and Bactec standard anaerobic/F bottl
239 s undertaken to determine the utility of the BACTEC Peds Plus/F bottle and the BACTEC 9240 instrument
241 lts indicate the superior performance of the BACTEC Peds Plus/F bottle over the conventional agar pla
243 were produced using 50 Candida isolates and BACTEC Plus Aerobic/F and Anaerobic/F blood culture bott
246 s, the BACTEC system signaled that a bottle (BACTEC Plus Aerobic/F bottle or BACTEC Anaerobic Lytic/1
247 ential pathogen recovery was greater for the BACTEC Plus Aerobic/F bottle than for either the Isolato
248 BACTEC bottles: the MYCO/F Lytic bottle, the BACTEC Plus Aerobic/F bottle, and the BACTEC Anaerobic L
249 imal anaerobic companion bottle to pair with BACTEC Plus Aerobic/F medium for recovery of pathogenic
250 the BD Bactec FX blood culture (BC) system (Bactec Plus Aerobic/F medium) and the VersaTREK system (
251 cology media for each system were evaluated: Bactec Plus Aerobic/F, Plus Anaerobic/F, and Myco/F Lyti
252 a level I trauma center was placed in paired BACTEC Plus and BacT/Alert FAN culture media and studied
253 For direct testing, an aliquot taken from BD BACTEC Plus and/or BD BACTEC Lytic/10 bottles (Becton Di
254 the ability of Becton Dickinson (Sparks, MD) BACTEC PLUS bottles and bioMerieux (Durham, NC) BacT/Ale
256 ere 15 (+/-1) days (range, 4 to 27 days) for BACTEC plus MTD and 19 (+/-1) days (range, 6 to 36 days)
257 ntrol bottles (no antibiotic added), and the BACTEC PLUS system recovered 95.1% of strains from test
259 in four medical centers were inoculated into BACTEC Plus/F and BacT/Alert FAN aerobic culture bottles
261 om previously published studies in which the BACTEC radiometric culture system had not been used.
263 trains (n = 34), were performed by using the Bactec radiometric growth system as the reference method
264 for repeat susceptibility testing using the BACTEC radiometric method and to the Centers for Disease
265 nclude that among the methods evaluated, the BACTEC radiometric method appeared to be the best for de
267 od were assessed, it was determined that the BACTEC resin bottle detected statistically significantly
268 tion for all pathogens combined favoring the BACTEC resin bottle over the Isolator tube (P < 0.05).
270 ation of pathogens in Bactec Peds Plus/F and Bactec standard anaerobic/F bottles that contained blood
273 n were seen with streptococci (10.7 h by the BACTEC system and 17.9 h by the BacT/Alert system) and y
276 red cells and whole blood were compared, the Bactec system detected bacterial growth consistently soo
280 /ALERT system with FA and FN bottles and the BACTEC system with Plus (PL) and Lytic 10 (LY) bottles.
283 (MGIT) system has a liquid medium, like the BACTEC system, and does not require needles when inocula
284 ation to the species level was less with the BACTEC system, but this result was statistically signifi
290 instances, growth was detected first in the BACTEC system; in 12 cases, growth registered first in t
291 compared to those of mycobacterial culture (BACTEC TB 460 and Middlebrook 7H11 biplates), smear for
293 Clinical isolates also were tested with the BACTEC TB 460 system; these results agreed with those ob
296 ys for ESP II; 14.4, 16.6, and 12.1 days for BACTEC TB; and 17.8, 18.3, and 18.8 days for Middlebrook
299 lection, the odds of that culture growing in BACTEC were 4.8- and 5.2-fold greater, respectively, tha
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