ライフサイエンスコーパス: BFP

1 BFP are polymers of bundlin, a pilin protein that is enc

2 BFP filament biogenesis and function are encoded by the

3 BFP in any form has no bioluminescence or riboflavin-syn

4 BFP is a different protein again, and in the bacterial l

5 BFP production is not constitutive, but instead occurs u

6 BFP RPR was defined as a reactive RPR and a nonreactive

7 BFP subsequently underwent a striking alteration in stru

8 BFP was expressed initially as approximately 40 nm diame

9 BFP, a plasmid-encoded type IV bundle-forming pilus prod

10 Of 112 BFP reactors, 35 (31%) had at least one RPR test reactiv

11 from the EAF plasmid, confers LA ability and BFP biogenesis.

12 ar-localized GFP-human papillomavirus E6 and BFP-Bcl-2 did not interact when coexpressed in the same

13 We found that ECP and BFP structures can be simultaneously observed in the cou

14 ion sequence, DEVD, was used to link GFP and BFP together.

15 tochondrially localized cytochrome c-GFP and BFP-Bcl-2 showed little or no FRET, while nuclear-locali

16 e that mutants of GFP, such as the rsGFP and BFP, have been used as quantitative labels for the devel

17 Adhesins such as BFP and EspA, important in microcolony formation on epit

18 dlin expression or processing but block both BFP biogenesis and LA.

19 by having at least 2 visits with consistent BFP at all visits.

20 However, previously described "BFP" reporters have limited utility, primarily due to re

21 iously shown to encode functions that direct BFP biosynthesis.

22 ese adhesins in biofilms, the genes encoding BFP and EspA are expressed during biofilm formation.

23 Relative to controls, US exposure enhanced BFP expression measured via fluorescence 12-fold for pla

24 Mutants that do not express BFP or EspA form more-diffuse biofilms than does the wil

25 reby demonstrating that BfpE is required for BFP biogenesis.

26 The N-terminal sequence for BFP shows similarities to those of the YFP proteins and

27 utoaggregate, adhere to HEp-2 cells, or form BFP, thereby demonstrating that BfpE is required for BFP

28 situ, and the complex was then purified from BFP-expressing EPEC by a combination of nickel- and BfpB

29 epolarization following energy transfer from BFP to GFP.

30 e, green and blue fluorescent proteins (GFP, BFP), and Escherichia coli cells expressing GFP in silic

31 Of 711 patients with HIV, 96 (13.5%) had BFP tests and 342 (48.1%) had syphilis.

32 To learn more about how BFP protein expression is induced during epithelial-cell

33 the pathogenic role of BFP by explaining how BFP production would commence in the small intestine and

34 table BFPs offers opportunities for improved BFP signal discrimination vs background, greatly enhanci

35 To test the role of BfpF in BFP function and EPEC pathogenesis, two different mutati

36 ses BfpD and BfpF play antagonistic roles in BFP biogenesis and retraction, respectively, by interact

37 oscopy to localize the wild-type and mutated BFP-Ndk proteins in the cell.

38 k::Cm knockout mutant containing the mutated BFP-Ndk protein fusion.

39 rties with an existing construct (GFP-myosin-BFP), containing a green fluorescent protein acceptor an

40 single point mutations in the nonmodulatable BFP, mKalama1, creates a modulatable variant.

41 last gene of the bfp cluster, neither LA nor BFP biogenesis is conferred.

42 ct the synthesis of a morphologically normal BFP filament.

43 mation on epithelial cells, the adherence of BFP-deficient mutants is significantly abrogated, but th

44 4 genes are sufficient for the biogenesis of BFP in a heterologous E. coli host.

45 iscrete assembly complex that is composed of BFP proteins in stoichiometric amounts.

46 No evidence of BFP disappearance was found after prolonged infection.

47 ure media, indicating that the expression of BFP may be environmentally regulated.

48 Expression of BFP, a member of the type IV pilus family, requires the

49 er of 14 genes is required for expression of BFP.

50 und negatively to regulate the expression of BFP.

51 cells due to background expression levels of BFP and intimin.

52 enable long-wavelength optical modulation of BFP emission.

53 e was also associated with decreased odds of BFP persistence (AOR, 0.07; 95% CI, .01-.33).

54 use of cART appears to decrease the odds of BFP RPR tests.

55 RT use was associated with decreased odds of BFP tests compared to having syphilis (adjusted odds rat

56 g use were associated with increased odds of BFP.

57 ecpA single mutants, even in the presence of BFP.

58 dings further support the pathogenic role of BFP by explaining how BFP production would commence in t

59 To examine the role of BFP in EPEC aggregation and dispersal, we examined HEp-2

60 ith dramatic alterations in the structure of BFP bundles.

61 encodes the principal structural subunit of BFP.

62 Bacterial dispersal and transformation of BFP from thin to thick bundles did not occur with a bfpF

63 s was associated with this transformation of BFP from thin to thick bundles.

64 The utility of BFP in bicyclomycin-rho binding studies was documented t

65 to determine the effect of each mutation on BFP biogenesis and LA.

66 ver, isogenic mutants not expressing EspA or BFP were significantly less adherent when the ecpA gene

67 homogeneous populations of peptide-rsGFP or -BFP conjugates were produced.

68 A persistent BFP test was defined by having at least 2 visits with co

69 Twenty-two of 96 (23%) had persistent BFP tests.

70 ved in the synthesis of bundle-forming pili (BFP) are positively regulated by the plasmid-encoded reg

71 Production of type IV bundle-forming pili (BFP) by enteropathogenic Escherichia coli (EPEC) require

72 The bundle-forming pili (BFP) of enteropathogenic Escherichia coil (EPEC) are req

73 The type IV bundle-forming pili (BFP) of enteropathogenic Escherichia coli (EPEC) are req

74 The bundle-forming pili (BFP) of enteropathogenic Escherichia coli are believed t

75 l the expression of the bundle-forming pili (BFP), intimin, the type III secretion apparatus and the

76 (EPEC) strains produce bundle-forming pili (BFP), type IVB fimbriae that have been implicated in EPE

77 dhesins, including the bundle-forming pilus (BFP) and the EspA filament.

78 genic Escherichia coli bundle-forming pilus (BFP) biogenesis machine and characterized its ATPase act

79 might be required for bundle-forming pilus (BFP) biosynthesis.

80 e Shiga toxin genes or bundle-forming pilus (BFP) genes.

81 The bundle-forming pilus (BFP) of enteropathogenic Escherichia coli (EPEC) is a pr

82 The bundle-forming pilus (BFP) of enteropathogenic Escherichia coli (EPEC) is an i

83 Expression of the bundle-forming pilus (BFP) of enteropathogenic Escherichia coli (EPEC) is regu

84 Although the bundle-forming pilus (BFP) of enteropathogenic Escherichia coli (EPEC) mediate

85 Using the bundle-forming pilus (BFP) of enteropathogenic Escherichia coli as a model Tfp

86 V fimbria known as the bundle-forming pilus (BFP) that is required for autoaggregation and localized

87 -IV fimbria called the bundle-forming pilus (BFP), a prepilin peptidase necessary for processing of p

88 li (EPEC) produces the bundle-forming pilus (BFP), a type IV fimbria that has been implicated in viru

89 lence factors, such as bundle-forming pilus (BFP), EPEC secreted protein A, and other EPEC secreted p

90 d type IV fimbria, the bundle-forming pilus (BFP), is associated with the LA phenotype.

91 IV fimbria, termed the bundle-forming pilus (BFP).

92 mmunosuppression on biologic false-positive (BFP) rapid plasma reagin (RPR) tests among persons infec

93 for any high-titer biologic false-positive (BFP) reactors, that is, persons with rapid plasma reagin

94 .1-driven blue fluorescent protein-positive (BFP(+)) populations.

95 ocks signal sequence cleavage of prebundlin, BFP biogenesis, and LA.

96 a series of bicyclomycin fluorescent probes (BFP) constructed to sense the 1.rho interaction are desc

97 rebundlin expression, prebundlin processing, BFP biogenesis, or LA.

98 or localized adherence phenotype or produce BFP filaments.

99 n sequences express bundlin protein, produce BFP, and carry out autoaggregation and LA.

100 ibityllumazine; a blue fluorescence protein (BFP) with bound 6,7-dimethyl-8-ribityllumazine, riboflav

101 strate consists of blue fluorescent protein (BFP) and green fluorescent protein (GFP) flanking SNAP-2

102 y transfer between blue fluorescent protein (BFP) and green fluorescent protein (GFP) moieties linked

103 protein (GFP) and blue fluorescent protein (BFP) are covalently linked together by a short peptide.

104 ein of Ndk and the blue fluorescent protein (BFP) as well as a fusion protein of mutated Ndk (whose D

105 line DLD-1, and a blue fluorescent protein (BFP) expression vector was introduced into an isogenic d

106 The utility of blue fluorescent protein (BFP) has been limited by its low quantum yield and rapid

107 novirus-expressing blue fluorescent protein (BFP) or phosphate buffered saline.

108 oexpression of RXR-blue fluorescent protein (BFP) promoted nuclear accumulation of GFP-VDR by influen

109 tein (rsGFP) and a blue fluorescent protein (BFP) was developed for the detection of two model peptid

110 tein (GFP)-Bax and blue fluorescent protein (BFP)-Bcl-2 fusion proteins coexpressed in the same cell,

111 nce of pJPN14, is sufficient to reconstitute BFP biogenesis in a laboratory E. coli strain, but is in

112 er of 14 genes is sufficient to reconstitute BFP biogenesis in a laboratory strain of E. coli.

113 RXR-BFP also promoted hormone-dependent nuclear accumulation

114 nstrated that the unliganded GFP-VDR and RXR-BFP form heterodimers.

115 allidum-specific antigens, suggest that some BFP reactions may represent FTA-negative syphilis.

116 It is concluded that BFP promotes both the formation and the dispersal of EPE

117 findings in other T4P systems, we found that BFP components predominantly have an uneven distribution

118 We report that BFP biogenesis probably requires interactions among BfpC

119 The BFP assembly complex, containing a BfpB-His6 fusion prot

120 The BFP system should prove to be a useful model for studyin

121 DTEV motif has been changed to AAAA) and the BFP.

122 ergent alleles of bfpA encoding bundlin, the BFP pilin protein, in pilus biogenesis, pilus interactio

123 rence factor (EAF) plasmids, which carry the BFP genes, demonstrated significant plasmid diversity ev

124 light at 387 nm, which primarily excites the BFP, whereas emission from the GFP is monitored at 509 n

125 comparable to those of HB101 expressing the BFP.

126 Energy transfer from the BFP to the GFP in the intact substrate results in a subs

127 , indicating that BfpB acts at a step in the BFP biogenic pathway after production and processing of

128 s and their role in the stabilization of the BFP assembly complex.

129 by interacting with distinct domains of the BFP biogenesis machine.

130 asmic membrane proteins BfpC and BfpE of the BFP biogenesis machine.

131 in interactions required for assembly of the BFP biogenesis machinery.

132 encoding the major structural subunit of the BFP, abolish LA.

133 , excited near the absorption maximum of the BFP, is very low due to depolarization following energy

134 fused to either the gene of the rsGFP or the BFP, as desired.

135 s, mutations to the residues surrounding the BFP chromophore enable long-wavelength optical modulatio

136 We found that the BFP biogenesis machine from an EPEC strain that expresse

137 This BFP, named Azurite, is well expressed in bacterial and m

138 tterns over time, along with very high-titer BFP reactions and reactivity with T. pallidum-specific a

139 ::Cm knockout mutant harboring the wild-type BFP-Ndk protein fusion.

140 the isolated complex by immunoblotting using BFP protein-specific antibodies showed that at least 10

141 A blue-shifted GFP variant (BFP) and a red fluorescent protein (DsRed) were also use

142 eractions among BfpC, BfpD and BfpE, whereas BFP retraction requires interaction of the PilT-like put

143 or HIV RNA was significantly associated with BFP test results.

144 n accessory factor that, in association with BFP and other adhesins, contributes to the multifactoria

145 Patients with BFP tests were compared to 2 control groups: HIV-infecte