ライフサイエンスコーパス: BLV

1 BLV can be transmitted in cocultures to adherent suscept

2 BLV infection is strongly associated with B-cell tumors

3 BLV is closely related to the human T-cell leukemia viru

4 BLV tax and pol mRNA levels increased in transwell cultu

5 BLV viremia was assessed by spontaneous lymphocyte proli

6 PBMCs from 3 of 10 BLV-infected sheep displayed a lifelong deficiency in in

7 In seropositive cows without PL (n=14), BLV-infected CD5+ and CD5- B cells accounted for 9.2% +/

8 In cows with PL (n=5), BLV-infected CD5+ and CD5- B cells accounted for 66% +/-

9 fectivity of the simple BLV derivatives in a BLV animal model.

10 tutive in vivo protein-DNA interactions in a BLV-producing cell line, Bat2Cl6.

11 Overexpression of a BLV Gag polyprotein containing a carboxy-terminal influe

12 Soon after BLV injection, immunoglobulin M+ (IgM+) and CD8+ cells i

13 e of cytotoxic T-lymphocyte response against BLV envelope proteins (Env; gp51/gp30).

14 d blood cells were cultured briefly to allow BLV expression.

15 sults from both the compensatory mutants and BLV-HTLV chimeras indicate that the encapsidation sequen

16 a correlation between cell proliferation and BLV expression, the effect of IL-2 and IL-10 on PBMC pro

17 clonal anti-rhIL-2 antibody, as well as anti-BLV antibody, inhibited spontaneous proliferation of per

18 om PL and TB animals failed to recognize any BLV recombinant proteins.

19 ne the possibility to obtain Ca biofortified BLV by using agronomic approaches.

20 For the production of biofortified BLV, a floating system with two level of Ca (100 and 200

21 Although both BLV and HTLV-1 infection are characterised by large expa

22 iferation to recombinant proteins encoded by BLV gag (p12, p15, and p24) and env (gp30, and gp51) gen

23 immune responses stimulate BLV expression by BLV-infected B cells.

24 n, these data suggest that cell infection by BLV is a multistep process requiring receptor binding (i

25 Thus, IL-10 production by BLV-infected animals with late stage disease may serve t

26 irus and gag-pol or env genes of the complex BLV.

27 with late stage disease may serve to control BLV mRNA levels, while IL-2 may increase BLV mRNA in the

28 that virus neutralization by MAbs to defined BLV gp51 epitopes can occur subsequent to virus engageme

29 ed apparent bone lesion volume change (Delta BLV).

30 assessment had a significantly higher Delta BLV than those without.

31 te response may be responsible for directing BLV pathogenesis, this possibility has been left largely

32 Here, we demonstrate that each BLV pre-miRNA is directly transcribed by RNAP III from i

33 inimum-energy optimal folding for the entire BLV RNA, including the previously mapped primary and sec

34 ted population of permeable cells expressing BLV and inhibited BLV replication in a culture of bovine

35 StxA1, and at various times cells expressing BLV were identified by being stained with MW1 monoclonal

36 ffects of these cytokines on BLV expression, BLV tax and pol mRNA and p24 protein were quantified by

37 In contrast, in CD5(+) B cells from BLV-infected PL cattle, CD5 was dissociated from the BCR

38 e of peripheral blood mononuclear cells from BLV-infected sheep was expressed in COS-1 cells and test

39 Interestingly, culture of cells from BLV-negative or persistently lymphocytic cattle failed t

40 PBMC from BLV-positive animals invariably displayed spontaneous ly

41 The toxin activity in PBMC from BLV-positive cattle was selective for viral SLP and did

42 Sera from BLV-infected animals possess high BLV-neutralizing antib

43 Sera from BLV-infected animals possess high BLV-neutralizing antibody titres.

44 little viral mRNAs or proteins, exactly how BLV contributes to tumorigenesis has remained a decades-

45 er of the human T-cell leukemia virus (HTLV)/BLV group of retroviruses.

46 emia virus (BLV), another member of the HTLV/BLV genus of retroviruses, and is about fourfold lower t

47 e cis-acting elements of a virus of the HTLV/BLV group.

48 increased T-lymphocyte expression of IL-2 in BLV-infected cows contributes to development and/or main

49 Because most cells in BLV-associated tumors express little viral mRNAs or prot

50 ne lymphotropic herpesvirus as a cofactor in BLV pathogenesis is considered.

51 nonneoplastic from neoplastic conditions in BLV-infected cattle.

52 interfilament lattice spacing is greater in BLV than in BLA and that the lattice spacing is coupled

53 MA alpha-helix II, previously implicated in BLV gRNA packaging, reduces NA binding affinity.

54 -10 inhibited BLV tax and pol mRNA levels in BLV-infected PBMCs; however, the inhibitory effect of IL

55 virus expression and disease progression in BLV infection.

56 serves as a marker of disease progression in BLV-infected cattle but is not necessarily associated wi

57 activity and may serve an important role in BLV-infected cattle by inhibiting BLV replication and th

58 rol BLV mRNA levels, while IL-2 may increase BLV mRNA in the early disease stage.

59 In contrast, IL-2 increased BLV tax and pol mRNA and p24 protein production.

60 Addition of PGE(2) increased BLV tax mRNA regardless of NS-398 addition.

61 ges secrete soluble factor(s) that increases BLV mRNA levels and that secretion of these soluble fact

62 IL-10 inhibited BLV tax and pol mRNA levels in BLV-infected PBMCs; howev

63 permeable cells expressing BLV and inhibited BLV replication in a culture of bovine peripheral blood

64 nt role in BLV-infected cattle by inhibiting BLV replication and thus slowing the progression of infe

65 the host immune system, IL-10 also inhibits BLV tax and pol mRNA levels in vitro.

66 leukemia virus (BLV) infection, we injected BLV-infected or mock-infected allogeneic cells into the

67 hereas in type C retroviruses (lentiviruses, BLV/HTLV group) Gag is targeted efficiently to the plasm

68 uclear cells from persistently lymphocytotic BLV-infected cows, nonlymphocytotic BLV-infected cows, a

69 ex, we generated a reporter assay to measure BLV Rex function and used it to screen a series of point

70 One BLV miRNA, BLV-miR-B4, shares partial sequence identity and shared

71 acteria have antiviral activity and mitigate BLV-induced disease.

72 horylated on serine residues, but the native BLV Env protein was not phosphorylated either in transfe

73 may serve a surveillance role during natural BLV infection.

74 Overexpression of the dominant-negative BLV Rex altered the localization of the wild-type protei

75 In contrast, the dominant-negative BLV Rex mutation had a diffuse nuclear localization and

76 uctively infected cells, indicating that new BLV infections stimulate proliferation of two different

77 ocytotic BLV-infected cows, nonlymphocytotic BLV-infected cows, and uninfected cows.

78 2-induced proliferation of PBMC from normal (BLV-negative) cows and had no effect on concanavalin A-i

79 in 53% (18 of 34) of hematologically normal, BLV-seropositive cattle and in 100% (10 of 10) of BLV-se

80 eropositive cattle and in 100% (10 of 10) of BLV-seropositive cattle with the preneoplastic syndrome

81 leukemia virus (BLV) (n = 63) and in 87% of BLV-seronegative animals (n = 38).

82 2 inhibitor, NS-398, inhibited the amount of BLV mRNA detected.

83 tivity that is involved in the biogenesis of BLV miRNAs and shRNA-generated sRNAs.

84 Rare mononuclear cells acting as centers of BLV infection in culture were present within 4 to 6 days

85 aneous lymphoproliferation characteristic of BLV-induced persistent lymphocytosis is IL-2 dependent a

86 This dominant-negative derivative of BLV Rex could be a useful tool to test the concept of in

87 Systemic dissemination of BLV-infected cells was thus accompanied by an increase i

88 ed but did not prohibit the establishment of BLV infection in vivo.

89 Stx1A inhibited expression of BLV p24 protein by PBMC.

90 r, we identified a dominant-negative form of BLV Rex.

91 ability clustered in the N-terminal half of BLV SU, which forms the putative receptor-binding domain

92 and IL-10 addition inhibited the increase of BLV tax and pol mRNA.

93 levels and reversed the IL-10 inhibition of BLV mRNA.

94 Using inhibition of BLV-dependent spontaneous lymphocyte proliferation as a

95 lly stimulated B cells may be a mechanism of BLV-induced PL.

96 ddress this, alanine-scanning mutagenesis of BLV PrGag was done with a virus-like particle (VLP) syst

97 ytometric analysis showed that the number of BLV-expressing cells were specifically reduced in cultur

98 ymphocytes showed sharply reduced numbers of BLV particles in StxA1-treated cultures.

99 in proliferating CD8+ cells and the onset of BLV-specific antibodies in lymph.

100 the impact of STEC on the initial phases of BLV infection in sheep.

101 low cytometry and tested for the presence of BLV by single-cell PCR.

102 ble to identify the nuclear export signal of BLV Rex.

103 hus, for the first time, advancing stages of BLV infection were correlated with decreased T-cell comp

104 stimulation, suggesting that stimulation of BLV tax and pol mRNA levels by PGE(2) is independent of

105 n shown to be the primary cellular target of BLV, recent studies suggest that some T lymphocytes and

106 Here, we show that SU and TM of BLV do, indeed, associate through disulfide bonds, wheth

107 To gain a better understanding of BLV Rex, we generated a reporter assay to measure BLV Re

108 The inhibitory effect of IL-10 on BLV expression depends on soluble factors secreted by ma

109 To examine the effects of these cytokines on BLV expression, BLV tax and pol mRNA and p24 protein wer

110 One BLV miRNA, BLV-miR-B4, shares partial sequence identity

111 Average weight gain post-BLV challenge was higher in STEC-treated sheep than in u

112 lymphoid cell line constitutively producing BLV.

113 T lymphocytes from only AL cattle recognize BLV Env without a requirement for classical major histoc

114 The availability of the recombinant BLV receptor candidate, BLVRcp1, allowed us to determine

115 (COX-2) product of macrophages, may regulate BLV expression.

116 sociated with B-cell neoplasms that resemble BLV-associated tumors, our findings suggest a possible m

117 ts infected with the first-generation simple BLV coviruses, the second-generation replication-compete

118 Here we describe a second-generation simple BLV derivative that is encoded on a single hybrid genome

119 The first-generation simple BLV derivatives replicate as complementary viruses (covi

120 These results establish that simple BLV derivatives lacking tax and rex are infectious and i

121 immunogenicity and infectivity of the simple BLV derivatives in a BLV animal model.

122 ood mononuclear cells (PBMC), and the simple BLV derivatives were also found to infect PBMC as demons

123 le IL-2-activated immune responses stimulate BLV expression by BLV-infected B cells.

124 Addition of PGE(2) stimulated BLV tax and pol mRNA levels and reversed the IL-10 inhib

125 In contrast, IL-2 stimulates BLV tax and pol mRNA and p24 protein expression in cultu

126 production in late disease stages suppresses BLV mRNA levels, while IL-2-activated immune responses s

127 r reverse transcription of newly synthesized BLV RNA.

128 d small RNA sequencing data demonstrate that BLV 5p miRNAs are co-terminal with 5'-triphosphorylated

129 d a full-length 64-kDa protein, but both the BLV and WRV VLPs also contained a 58-kDa protein that re

130 ect evidence for the involvement of both the BLV MA and NC domains of PrGag in viral RNA packaging.

131 blood monocytes or T lymphocytes contain the BLV provirus in seropositive cows with or without PL.

132 ombinant vaccinia virus (rVV) delivering the BLV env gene ranged from 30 to 65%.

133 ith MW1 monoclonal antibody specific for the BLV protein gp51.

134 ever, monoclonal antibodies specific for the BLV surface glycoprotein did not stain fixed PBMCs of th

135 The alteration in the BLV genome delayed but did not prohibit the establishmen

136 tingly, when conserved basic residues in the BLV MA domain of PrGag were mutated to alanine or glycin

137 s residues of the zinc finger domains in the BLV NC domain of PrGag revealed residues that led to a r

138 We modeled the BLV RBD by aligning putative structural elements with kn

139 important for Tax-mediated activation of the BLV LTR, we found footprints in regions flanking these e

140 we sought to clarify the distribution of the BLV provirus in subpopulations of peripheral blood monon

141 icate in tissue culture independently of the BLV regulatory proteins, Tax and Rex, and the RIII and G

142 Replacement of the BLV RNA region containing the primary and secondary enca

143 We show that the middle region of the BLV U3 contains multiple dual-functioning cis-acting ele

144 I-generated short hairpin RNAs (shRNAs), the BLV pre-miRNAs are initially 5'-triphosphorylated.

145 Thus, the BLV miRNAs avoid the conundrum of genome/mRNA cleavage b

146 A similar observation was made with the BLV PPPY motif.

147 s, both dileucine and YXXL motifs within the BLV CTM contribute to downmodulation of a protein contai

148 s but one are exposed on the surface of this BLV model.

149 suggest a possible mechanism contributing to BLV-induced tumorigenesis.

150 Cells proliferating to BLV antigens were CD4+ T lymphocytes, as shown by cell d

151 ocalization analysis revealed that wild-type BLV Rex had a punctate nuclear localization and was asso

152 Wild-type BLV typically infects peripheral blood mononuclear cells

153 plication-competent derivative, or wild-type BLV.

154 Interestingly, unintegrated BLV DNA was not detected in tumor cells from cattle with

155 Thus, the presence of unintegrated BLV DNA differentiated nonneoplastic from neoplastic con

156 suggested that accumulation of unintegrated BLV DNA resulted from a process of reinfection rather th

157 The concomitant presence of unintegrated BLV DNA with viral transcriptional activity was observed

158 We have used BLV Env protein variants to gain insights into the struc

159 increase Ca content of baby leaf vegetables (BLV: basil, mizuna, tatsoi and endive), as fresh-cut pro

160 udied muscle from the bovine left ventricle (BLV), which expresses a high level of a stiff titin isof

161 lly distinct viruses, Burwash Landing virus (BLV), White River virus (WRV), and Florida virus.

162 ion structure of the bovine leukaemia virus (BLV) matrix protein by heteronuclear nuclear magnetic re

163 LV-1 and HTLV-2) and bovine leukaemia virus (BLV).

164 were seropositive for bovine leukemia virus (BLV) (n = 63) and in 87% of BLV-seronegative animals (n

165 iruses, which include bovine leukemia virus (BLV) and human T-cell leukemia virus type 1 (HTLV-1) and

166 iruses, which include bovine leukemia virus (BLV) and human T-cell leukemia virus types 1 and 2 (HTLV

167 : sheep infected with Bovine Leukemia Virus (BLV) and humans infected with Human T Lymphotropic Virus

168 Env) glycoproteins of bovine leukemia virus (BLV) and its close relative, human T-cell leukemia virus

169 In contrast, bovine leukemia virus (BLV) expresses subgenomic RNAP III transcripts that give

170 arly establishment of bovine leukemia virus (BLV) infection, we injected BLV-infected or mock-infecte

171 arly disease stage of bovine leukemia virus (BLV) infection, while IL-10 increases in animals with a

172 ate disease stages of bovine leukemia virus (BLV) infection.

173 SU and TM proteins of bovine leukemia virus (BLV) initially were reported to be disulfide linked but

174 Bovine leukemia virus (BLV) is a complex B-lymphotrophic retrovirus of cattle a

175 Bovine leukemia virus (BLV) is a member of the human T-cell leukemia virus (HTL

176 The bovine leukemia virus (BLV) is an oncogenic retrovirus that is associated with

177 The Rex of bovine leukemia virus (BLV) is poorly characterized.

178 n the deltaretrovirus bovine leukemia virus (BLV) matrix (MA) and NC domains affects virus replicatio

179 oncogenic retrovirus bovine leukemia virus (BLV) produce virus when cultured briefly.

180 simple derivatives of bovine leukemia virus (BLV) that can replicate in tissue culture independently

181 e correlation between bovine leukemia virus (BLV) unintegrated DNA, viral expression, and stage of di

182 capsidation signal of bovine leukemia virus (BLV) was previously shown by deletion analysis to be dis

183 e TM protein (CTM) of bovine leukemia virus (BLV) was regulated by two membrane-proximal dileucine mo

184 Bovine leukemia virus (BLV), a retrovirus related to human T-cell leukemia viru

185 demonstrate that the bovine leukemia virus (BLV), a retrovirus with an RNA genome, encodes a conserv

186 Bovine leukemia virus (BLV), a transactivating lymphotropic retrovirus, is the

187 comparable to that of bovine leukemia virus (BLV), another member of the HTLV/BLV genus of retrovirus

188 in (Stx) acts against bovine leukemia virus (BLV)-expressing cells was obtained.

189 Bovine leukemia virus (BLV)-induced persistent lymphocytosis is characterized b

190 Stx activity against bovine leukemia virus (BLV)-infected cells in vitro and hypothesized that STEC

191 has activity against bovine leukemia virus (BLV).

192 assembly pathway for bovine leukemia virus (BLV).

193 om cows infected with bovine leukemia virus (BLV).

194 cattle infected with bovine leukemia virus (BLV; a retrovirus closely related to human T-cell leukem

195 not detected in tumor cells from cattle with BLV-associated lymphocytic leukemia/malignant lymphoma d

196 ne sera from animals naturally infected with BLV blocked gp51 binding to recombinant BLVRcp1.

197 determined in cattle naturally infected with BLV.

198 l blood that are significantly infected with BLV.

199 ing that both reagents likely interfere with BLV-dependent initiation of SLP.

200 be useful tools in comparative studies with BLV to evaluate the role of tax and rex in maintenance o

201 ons of human CD8-alpha plus a wild-type (wt) BLV CTM was detectable on the surface of only 40% of the