ライフサイエンスコーパス: C. crescentus

1 C. crescentus cells attached to a surface undergo Browni

2 C. crescentus GrpE, shown to be essential for viability

3 C. crescentus is, to our knowledge, the first free-livin

4 C. crescentus showed surprisingly high tolerance to uran

5 C. crescentus SspB shares limited sequence similarity wi

6 C. crescentus thus repurposes pilus retraction, typicall

7 d the molecular basis for this phenotype, 73 C. crescentus proteins were identified that are tagged b

8 Moreover, the survival of a C. crescentus katG null mutant is reduced by more than 3

9 us HisRS allowed complete histidylation of a C. crescentus tRNA(His) transcript (lacking G(-1)).

10 lleles unable to bind myo-inositol abolishes C. crescentus growth in medium containing myo-inositol a

11 polymorphic locus, zwf, between lab-adapted C. crescentus and clinical isolates of Pseudomonas aerug

12 high-affinity ribose binding allele affected C. crescentus growth on D-ribose as a carbon source, pro

13 We also used modified E. coli and C. crescentus ssrA tags to independently control the deg

14 Thus, in both R. meliloti and C. crescentus, CcrM methylation is an integral component

15 alpha subdivision bacteria, R. meliloti and C. crescentus, CcrM-mediated methylation has important c

16 regulons of CtrA and DnaA in S. meliloti and C. crescentus.

17 ter membrane complements of H. neptunium and C. crescentus are remarkably similar.

18 etwork is conserved between the rhizobia and C. crescentus, a free-living aerobe that cannot fix nitr

19 We report the identification of another C. crescentus heat shock operon containing two genes, hr

20 is expressed similarly to the autoregulated C. crescentus ctrA in that both genes have complex promo

21 length ccrM genes from the aquatic bacterium C. crescentus, the soil bacterium R. meliloti, and the i

22 The bacterium C. crescentus coordinates cellular differentiation and c

23 In the bacterium C. crescentus, the cellular homologs of plasmid partitio

24 ility, and stationary-phase survival between C. crescentus strain CB15 and its derivative NA1000 is d

25 n of G(-1) did not improve aminoacylation by C. crescentus HisRS.

26 confirmed that these species are cleared by C. crescentus YbaK and ProXp-ala, respectively.

27 nosa, Escherichia coli, and Vibrio cholerae, C. crescentus CB15 cells form biphasic biofilms, consist

28 Like the katG of Escherichia coli, C. crescentus katG is induced by hydrogen peroxide and i

29 In contrast, C. crescentus KatG activity is constant throughout expon

30 e show that, unlike its E. coli counterpart, C. crescentus RhlB interacts directly with a segment of

31 Consistent with its ecological distribution, C. crescentus displays a narrow range of osmotolerance,

32 we demonstrate that cell curvature enhances C. crescentus surface colonization in flow.

33 We exposed C. crescentus cells to four heavy metals (chromium, cadm

34 NA methyltransferase (CcrM) is essential for C. crescentus cellular viability.

35 These results indicate a major role for C. crescentus catalase-peroxidase in stationary-phase su

36 base U20a, created a competent substrate for C. crescentus HisRS.

37 a marine member of the DPB that differs from C. crescentus in that H. neptunium uses its stalk as a r

38 The enzyme from C. crescentus is a homodimeric, membrane-associated prot

39 the peptide side chains of PG isolated from C. crescentus cells grown in the complex laboratory medi

40 Furthermore, C. crescentus RNase E appears associated with the DNA in

41 Growing C. crescentus in the presence of blue light dramatically

42 In C. crescentus the CtrA response regulator serves as the

43 In C. crescentus, a P168V mutant is not activating in vivo,

44 In C. crescentus, ParB binds to sequences adjacent to the o

45 In C. crescentus, the directionality of the transport is se

46 In C. crescentus, the Fix network is required for normal ce

47 or kinases called pdhS1 and pdhS2, absent in C. crescentus.

48 ible for catalase and peroxidase activity in C. crescentus.

49 Rather, adhesion in C. crescentus is a complex developmental process.

50 oid-partitioning defects are not apparent in C. crescentus Topo IV mutants as they are in E. coli and

51 As in C. crescentus, the S. meliloti PodJ1 protein appears to

52 tumefaciens are vertically-integrated, as in C. crescentus.

53 FtsZ curvature and efficient constriction in C. crescentus.

54 h the logic of stringent response control in C. crescentus differs from E. coli, the global transcrip

55 ram to the asymmetric cell-division cycle in C. crescentus, studies of flagellar gene regulation and

56 tight temporal control of the cell cycle in C. crescentus.

57 to regulate the initiation of cytokinesis in C. crescentus.

58 rotease thus exhibits pleiotropic effects in C. crescentus growth and development.

59 Thus, the major recognition elements in C. crescentus tRNA(His) are the anticodon, the discrimin

60 rough mutational analysis to be essential in C. crescentus for growth on glucose.

61 cell cycle regulatory genes are essential in C. crescentus, the essential genes of two Alphaproteobac

62 nates cell cycle and developmental events in C. crescentus by regulating the level of CtrA phosphoryl

63 eely diffusing mRNA, and provide evidence in C. crescentus that this mRNA localization restricts ribo

64 oded copy of rpoH induced DnaK expression in C. crescentus cultures grown at 30 degrees C.

65 or inducible heterologous gene expression in C. crescentus.

66 l arrangements of FtsZ and FtsA filaments in C. crescentus and E. coli cells and inside constricting

67 fsA; flgH), a model for biofilm formation in C. crescentus is proposed.

68 mentous cells, which are frequently found in C. crescentus fliF mutants, the McpA-GFP fusion was obse

69 Loss of PflI function in C. crescentus results in an abnormally high frequency of

70 nscription of other early flagellar genes in C. crescentus.

71 egulation of the flagellar gene hierarchy in C. crescentus and consider regulatory mechanisms that ar

72 The podJ gene, originally identified in C. crescentus for its role in polar organelle developmen

73 vation-induced stress response identified in C. crescentus.

74 shed the response to heat shock induction in C. crescentus.

75 Examination of the hfsH deletion mutant in C. crescentus revealed that this strain synthesizes hold

76 attern is due to the low ParA copy number in C. crescentus cells.

77 hose chromosome I segregates like the one in C. crescentus and whose chromosome II like the one in E.

78 t mmpA and yaeL can complement each other in C. crescentus and E. coli, indicating functional conserv

79 ortant target of the Lon protease pathway in C. crescentus.

80 ed the first histidine phosphotransferase in C. crescentus, ShpA, and show that it too is required fo

81 s coordinately regulate stress physiology in C. crescentus.

82 ture is a novel function for type IV pili in C. crescentus.

83 e to the maintenance of cellular polarity in C. crescentus.

84 bstrates of the ClpP associated proteases in C. crescentus.

85 ework to understand regulated proteolysis in C. crescentus and show that RcdA is not an adaptor for C

86 The organization of this region in C. crescentus is similar to that in other bacteria, with

87 so transcriptionally cell cycle-regulated in C. crescentus.

88 responsible for developmental regulation in C. crescentus.

89 sms that control peptidoglycan remodeling in C. crescentus.

90 dies of oxidative and starvation stresses in C. crescentus were undertaken through a study of lacZ fu

91 FtsZ to the membrane and demonstrate that in C. crescentus, FzlC is one such membrane anchor.

92 We show that, in C. crescentus, the rodA gene is essential and that RodA

93 thways responding to heavy-metal toxicity in C. crescentus to provide insights for the possible appli

94 Remarkably, glycine incorporation into C. crescentus peptidoglycan occurred even in the presenc

95 Delivery of these plasmids into C. crescentus resulted in integration via homologous rec

96 The isolated C. crescentus gene complements the growth defect of an E

97 Genome sequencing has revealed many C. crescentus cell cycle genes are conserved in other Al

98 and external structures to the attachment of C. crescentus to abiotic surfaces.

99 We found that the attachment of C. crescentus to surfaces is cell cycle regulated and th

100 thoroughly characterized the composition of C. crescentus peptidoglycan by high-performance liquid c

101 we investigated the dynamics and control of C. crescentus biofilms developing on glass surfaces in a

102 When cultures of C. crescentus are grown for extended periods in complex

103 ing similarity between the division cycle of C. crescentus and that of A. tumefaciens, the functional

104 tory network that controls the cell cycle of C. crescentus and, presumably, of many other Alphaproteo

105 that although the structure and function of C. crescentus sigma32 appear to be very similar to those

106 to serving as a carbon source for growth of C. crescentus, this pentose may be interpreted as a sign

107 Because the natural lifestyle of C. crescentus intrinsically involves a surface-associate

108 We used fluorescence microscopy of C. crescentus expressing green fluorescent protein to tr

109 plays a role in the surface modification of C. crescentus, facilitating the uptake of nutrients duri

110 Topo IV mutants of C. crescentus are highly pinched at multiple sites (cell

111 Topo IV mutants of C. crescentus are unlike mutants of Escherichia coli and

112 s been adapted to the unique physiologies of C. crescentus and the nitrogen-fixing rhizobia.

113 role of sigma32 in the normal physiology of C. crescentus.

114 The crystal structure of a portion of C. crescentus RNase E encompassing the helicase-binding

115 from the sigma54-dependent fljK promoter of C. crescentus in the presence of the transcription activ

116 We report here the purification of C. crescentus RNA polymerase holoenzymes and resolution

117 We analyzed the adaptive response of C. crescentus swarmer cells to carbon starvation and fou

118 lts in connection with the possible roles of C. crescentus Topo IV in the regulation of cell division

119 tagenesis at the predicted catalytic site of C. crescentus HfsH phenocopied the DeltahfsH mutant and

120 Substrate specificity of C. crescentus ProXp-ala is determined, in part, by eleme

121 dence of power-law statistics in the tail of C. crescentus cell-size distribution, although there is

122 the A. tumefaciens pathway resembles that of C. crescentus there are specific differences including a

123 be regulated in a similar manner to that of C. crescentus, and that the outer membrane complements o

124 ilarity to the characterized motifs of other C. crescentus transcriptional regulators.

125 Paradoxically, C. crescentus curvature is robustly maintained in the wi

126 ir promoters are recognized by the principal C. crescentus sigma factor sigma73.

127 were reconstituted exclusively from purified C. crescentus core and sigma factors.

128 Here we show that recombinant C. crescentus HisRS allowed complete histidylation of a

129 ognized and transcribed by the reconstituted C. crescentus Esigma32 RNA polymerase holoenzyme.

130 the divergent (based on sequence similarity) C. crescentus HisRS.

131 ue, we measured the adhesion force of single C. crescentus cells attached to borosilicate substrates

132 In the sessile stage, C. crescentus is often found tightly attached to a surfa

133 Forward swimming C. crescentus swarmer cells tend to get physically trapp

134 entified the hfaA gene in the synchronizable C. crescentus strain CB15.

135 We show here that C. crescentus extracellular DNA (eDNA) inhibits the abil

136 Using these data, we show that C. crescentus displays aerotactic behavior by extending

137 We show that C. crescentus ParB, like its plasmid homologues, is comp

138 We show that C. crescentus ProRS can readily form Cys- and Ala-tRNA(P

139 Our findings suggest that C. crescentus is optimally adapted to low nutrient aquat

140 The C. crescentus orthologue of tipA was named skgA (station

141 The C. crescentus rpoH gene was transcribed from either of t

142 The C. crescentus sigma32 homolog, predicted to be a 33.7-kD

143 interplay between flagellar assembly and the C. crescentus cell cycle.

144 Based on comparisons between the C. crescentus strain CB15 wild type and its holdfast (hf

145 lar to those of its E. coli counterpart, the C. crescentus rpoH gene contains a novel promoter struct

146 Here, we deleted the C. crescentus gene encoding the beta-subunit of the IHF,

147 Finally, the C. crescentus and R. meliloti ccrM genes are functionall

148 Hence, the C. crescentus PG is able to retain its physiological fun

149 und containing a conditional mutation in the C. crescentus ftsA gene, an early cell division gene tha

150 how here that glycine incorporation into the C. crescentus PG depends on the presence of exogenous gl

151 ue experiments demonstrated that divE is the C. crescentus ftsA homolog and that the ftsZ gene maps i

152 ParB binds sequences near the C. crescentus origin of replication.

153 The clusters of the C. crescentus and M. tuberculosis ferrochelatases are li

154 ation, we have characterized a region of the C. crescentus chromosome containing genes that are all i

155 The principal components of the C. crescentus degradosome are the endoribonuclease RNase

156 the heat shock-regulated promoter P1 of the C. crescentus dnaK gene, and base pair substitutions in

157 The ability of the C. crescentus Esigma73 RNA polymerase to recognize E. co

158 e fliIJ operon is located in class II of the C. crescentus flagellar regulatory hierarchy, suggesting

159 liE genes were identified as a result of the C. crescentus genome sequencing project and encode the h

160 n from the sigma32-dependent promoter of the C. crescentus heat-shock gene dnaK.

161 Analysis of the C. crescentus homolog of dnaX, which in Escherichia coli

162 Enzymes of the C. crescentus pathway, including an NAD(+)-dependent xyl

163 qualitative and quantitative analysis of the C. crescentus PG by high-performance liquid chromatograp

164 ma73-dependent housekeeping promoters of the C. crescentus pleC and rsaA genes.

165 demonstrate a spatial diversification of the C. crescentus population into a sessile, "stem cell"-lik

166 a, the major transcriptional response of the C. crescentus rpoH gene to heat shock depends on positiv

167 In this paper, the elastic properties of the C. crescentus stalk and holdfast assembly were studied b

168 he Esigma73 holoenzyme, we overexpressed the C. crescentus rpoD gene in Escherichia coli and purified

169 basis of these results, we suggest that the C. crescentus divA-divB-divE(ftsA)-ftsZ gene cluster cor

170 Genome analysis revealed that the C. crescentus genome encodes a significantly higher numb

171 We derive from these results that the C. crescentus swarmer cells swim more efficiently than b

172 expressed almost continuously throughout the C. crescentus cell cycle, suggesting that coupling of fl

173 tein levels remained constant throughout the C. crescentus cell cycle.

174 n previously that restriction of CcrM to the C. crescentus predivisional cell is essential for normal

175 of an E. coli rpoH deletion mutant with the C. crescentus rpoH gene.

176 onse regulator LovR also function within the C. crescentus general stress pathway.

177 haproteobacterial species closely related to C. crescentus.

178 rn and a role in ClpX positioning similar to C. crescentus CpdR, suggesting a conserved function of C

179 ion-limited, oligotrophic environments where C. crescentus thrives.

180 multiple flagella and no prosthecae, whereas C. crescentus, a freshwater bacterium, has a single pola

181 Lysates of E. coli in which C. crescentus LpxI (CcLpxI) is overexpressed display hig

182 es that this organism shares more genes with C. crescentus than it does with Silicibacter pomeroyi (a