ライフサイエンスコーパス: C. diphtheriae

1 C. diphtheriae and C. ulcerans mutants defective in haem

2 C. diphtheriae C7(-) acquired iron from heme, hemoglobin

3 C. diphtheriae ciuE deletion mutants exhibited a defect

4 C. diphtheriae was also able to use human serum albumin

5 A C. diphtheriae mntA mutant grew as well as the wild type

6 A C. diphtheriae zur mutant was more sensitive to peroxide

7 In this study, two clones isolated from a C. diphtheriae chromosomal library were shown to activat

8 Four clones from a C. diphtheriae genomic library complemented several of t

9 gene complemented the mutant phenotypes of a C. diphtheriae DeltadtxR strain.

10 Two (9.5%) cases died, one due to a C. diphtheriae infection and one due to C. ulcerans.

11 e the source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydr

12 Although C. diphtheriae does not depend on other actinomycetal ge

13 mbrane when expressed in Escherichi coli and C. diphtheriae.

14 rences from the mammalian HO-1 and bacterial C. diphtheriae HO structures, which suggests a structura

15 ome corynephages, and DT is only produced by C. diphtheriae isolates that harbor tox+ phages.

16 the utilization of heme as an iron source by C. diphtheriae and that the heme oxygenase activity of H

17 se of the Hb-Hp complex as an iron source by C. diphtheriae requires multiple iron-regulated surface

18 ional host iron sources that are utilized by C. diphtheriae.

19 oter-lacZ fusion was dependent on the cloned C. diphtheriae chrA and chrS genes (chrAS), which encode

20 A search of the partially completed C. diphtheriae genome identified a gene, mntR, whose pre

21 briae, FimA, is expressed in corynebacteria, C. diphtheriae strain NCTC13129 polymerized FimA to form

22 None of the eight C. diphtheriae cases were fully immunized.

23 ened the need for laboratories to screen for C. diphtheriae.

24 A new IRP, designated IRP6, was cloned from C. diphtheriae by a SELEX-like procedure.

25 Total cellular RNA isolated from C. diphtheriae was used to identify the transcriptional

26 ional DtxR-regulated promoter/operators from C. diphtheriae designated IRP3, IRP4, and IRP5.

27 epidemic of 1993 to 1998 and 13 non-Georgian C. diphtheriae strains (10 Russian and 3 reference isola

28 In C. diphtheriae C7, optimal expression from the hmuO prom

29 Pilus assembly in C. diphtheriae requires the pilin motif and the C-termin

30 ompare and contrast galactan biosynthesis in C. diphtheriae and M. tuberculosis In each species, the

31 mined the precise amount of DtxR per cell in C. diphtheriae.

32 Transcription of the hmuO gene in C. diphtheriae is controlled under a dual regulatory mec

33 irst time that dtxR is a dispensable gene in C. diphtheriae.

34 Deletion of the hrtAB genes in C. diphtheriae produced increased sensitivity to hemin,

35 Inactivation of hmuT in C. diphtheriae by site-specific recombination had no eff

36 ism of the regulation of heme homeostasis in C. diphtheriae.

37 talloregulatory proteins to be identified in C. diphtheriae.

38 The use of hemin iron in C. diphtheriae involves the dtxR- and iron-regulated hmu

39 and characterized two novel genetic loci in C. diphtheriae that encode factors that bind hemin and H

40 of the general oxidative folding machine in C. diphtheriae.

41 indings demonstrated that the irp6 operon in C. diphtheriae encodes a putative ABC transporter, that

42 l Mn(2+) transport systems may be present in C. diphtheriae.

43 dependent activation at the hmuO promoter in C. diphtheriae; however, it was observed that significan

44 n and may function as the haemin receptor in C. diphtheriae.

45 function as cell surface hemin receptors in C. diphtheriae.

46 insights into transcriptional regulation in C. diphtheriae.

47 a zinc specific transcriptional regulator in C. diphtheriae and give new insights into the intricate

48 To further define the DtxR regulon in C. diphtheriae, a DtxR repressor titration assay (DRTA)

49 ered by a lack of molecular genetic tools in C. diphtheriae and related Coryneform species.

50 with a proposed mechanism of hemin uptake in C. diphtheriae in which hemin is initially obtained from

51 B had no effect on hemin iron utilization in C. diphtheriae.

52 n in hemoglobin-iron utilization, whereas in C. diphtheriae strains, deletion of hmuO caused no or on

53 delivered to the cytoplasm of non-lysogenic C. diphtheriae, they integrated into either the attB1 or

54 A total of 26 nontoxigenic C. diphtheriae strains isolated in the United Kingdom du

55 acterium diphtheriae strains, 9 nontoxigenic C. diphtheriae strains, and 44 strains representing the

56 esting classified the strain as nontoxigenic C. diphtheriae biotype Gravis.

57 udied the predominant strain of nontoxigenic C. diphtheriae circulating in the United Kingdom to see

58 monstrated that the majority (87.5%; 7/8) of C. diphtheriae strains represented new sequence types (S

59 he chtC gene has no affect on the ability of C. diphtheriae to use hemin or Hb as iron sources; howev

60 morphism [AFLP]) for the characterization of C. diphtheriae strains.

61 genes were constructed on the chromosome of C. diphtheriae.

62 Here, we characterized a large collection of C. diphtheriae clinical isolates for their pilin gene po

63 attP site and the DIP0182 integrase gene of C. diphtheriae NCTC13129.

64 e six sortase genes encoded in the genome of C. diphtheriae are required for precursor processing, pi

65 he organism showed that several genotypes of C. diphtheriae circulated on different continents of the

66 ntly was caused by one major clonal group of C. diphtheriae (PFGE type A, ribotype R1), which was ide

67 A distinct clonal group of C. diphtheriae isolates (ET 8 complex) emerged in Russia

68 upported the improbability of importation of C. diphtheriae into this area and rather strongly sugges

69 Therefore, recent clinical isolates of C. diphtheriae produce a single antigenic type of DT, an

70 nd dtxR genes in recent clinical isolates of C. diphtheriae revealed several tox alleles that encode

71 pand the systems for genetic manipulation of C. diphtheriae, we constructed plasmid vectors capable o

72 a dtxR mutant of C7, and in a hmuO mutant of C. diphtheriae HC1 provided further evidence that transc

73 We report that an htaA deletion mutant of C. diphtheriae strain 1737 is unable to use the Hb-Hp co

74 Mutants of C. diphtheriae and Corynebacterium ulcerans that are def

75 play a crucial role in the pathogenicity of C. diphtheriae.

76 nd antigenically distinct from other pili of C. diphtheriae.

77 m the SpaABC pili and possibly other pili of C. diphtheriae.

78 BC transporter involved in the protection of C. diphtheriae from hemin toxicity.

79 infection caused by a nontoxigenic strain of C. diphtheriae and discuss the epidemiology, possible so

80 all eight tox regions identical to those of C. diphtheriae Park-Williams No. 8 (tox type 1).

81 Diagnostic tests for toxinogenicity of C. diphtheriae are based either on immunoassays or on bi

82 RAPD typing, ribotyping, and PFGE typing of C. diphtheriae strains were improved to enable rapid and

83 exotoxin produced by the causative organism, C. diphtheriae; this detection is the definitive test fo

84 istently, cell wall extracts of a particular C. diphtheriae strain (DSM43989) lacking mycolic acid es

85 We show that several C. diphtheriae strains use the hemoglobin-haptoglobin (H

86 The findings from this study indicate that C. diphtheriae contains at least 18 DtxR binding sites a

87 ct on hemin utilization, which suggests that C. diphtheriae has an additional system for transporting

88 rom heme and hemoglobin, which suggests that C. diphtheriae possesses a novel mechanism for utilizing

89 The C. diphtheriae GlfT2 gave rise to shorter polysaccharide

90 The C. diphtheriae hmuO gene encodes a heme oxygenase that i

91 his report, we identify and characterize the C. diphtheriae hrtAB genes, which encode a putative ABC

92 or each of these systems was cloned from the C. diphtheriae chromosome, and constructs each carrying

93 iron and heme whereas transcription from the C. diphtheriae hmuO promoter shows both significant iron

94 sid operon contained a large deletion in the C. diphtheriae C7 strain, but the sid genes were unaffec

95 tial alpha-meso-hydroxylation of heme in the C. diphtheriae heme oxygenase-catalyzed reaction.

96 rynephages are capable of inserting into the C. diphtheriae chromosome at two specific sites, attB1 a

97 A BLAST search of the C. diphtheriae genome identified a seven-gene cluster th

98 A BLAST search of the C. diphtheriae genome sequence revealed a second two-com

99 of the C. ulcerans mutants and three of the C. diphtheriae mutants.

100 ect on iron uptake or the utilization of the C. diphtheriae siderophore as an iron source.

101 volved in the synthesis and transport of the C. diphtheriae siderophore.

102 The hrtAB genes are located on the C. diphtheriae genome upstream from the chrSA operon, wh

103 re closely related to each other than to the C. diphtheriae strains isolated in other parts of the Un

104 n iron uptake and reduced ability to use the C. diphtheriae siderophore as an iron source.

105 wed significant genetic diversity within the C. diphtheriae species, and ribotyping and MEE data gene

106 Thus, C. diphtheriae employs a common sortase-catalysed mechan

107 ron-regulated promoters in vivo and binds to C. diphtheriae operators in a metal-dependent manner in

108 addresses the clone's origin and relation to C. diphtheriae from throughout Russia.

109 LST scheme, which is currently restricted to C. diphtheriae.

110 Toxigenic C. diphtheriae was isolated from five members of four ho

111 epresent a potential reservoir for toxigenic C. diphtheriae.

112 ial reservoir for the emergence of toxigenic C. diphtheriae strains if they possessed functional diph

113 The toxigenic C. diphtheriae isolate NCTC13129 produces three distinct

114 ssed under high-iron conditions in wild-type C. diphtheriae C7(beta), but they were expressed constit

115 s secretion of diphtheria toxin by wild-type C. diphtheriae.

116 is currently the method of choice for typing C. diphtheriae.

117 Hemin iron utilization assays using various C. diphtheriae mutants indicate that deletion of the cht

118 In a blinded test of 209 verified C. diphtheriae isolates, a 99.5% agreement with the stan

119 utative composite transposon associated with C. diphtheriae isolates that dominated the diphtheria ou

120 tants and on the results of experiments with C. diphtheriae genes cloned in Escherichia coli or analy

121 Protein localization studies with C. diphtheriae showed that HtaA is associated predominan

122 Tn5-based mutagenesis technique for use with C. diphtheriae, and we used it to construct the first tr