ライフサイエンスコーパス: C. reinhardtii

1 C. reinhardtii BBS4 may be required for the export of si

2 C. reinhardtii cells deprived of iron have more saturate

3 C. reinhardtii cells exposed to oxidative stress show in

4 C. reinhardtii insertional mutants defective in BBS1, -4

5 C. reinhardtii is serving as an important model organism

6 C. reinhardtii knockdown mutants for GPD2 and GPD3 showe

7 C. reinhardtii mutants null for cia5 do not express seve

8 C. reinhardtii Rubisco contains Leu-326 and Met-349, whe

9 ibodies raised to PsbW we have examined: (1) C. reinhardtii mutants lacking either photosystem and (2

10 A C. reinhardtii insertional mutant null for IFT46 has sho

11 Gene g5047 encodes a C. reinhardtii homolog of the chloroplast-localized SRP4

12 We have isolated a gene, Ccs1, from a C. reinhardtii genomic library that complements both the

13 scribe the cloning and characterization of a C. reinhardtii version of a TRP channel sharing key feat

14 for Rca was cloned and expressed in pSL18, a C. reinhardtii expression vector conferring paromomycin

15 An antibody raised against C. reinhardtii hydin was specific for an approximately 5

16 Under nitrogen deprivation, the green alga C. reinhardtii showed substantial triacylglycerol (TAG)

17 th a polyacrylate coating) by the green alga C. reinhardtii was investigated in order to assess the c

18 e absence of an active Rca in the green alga C. reinhardtii.

19 Although C. reinhardtii has a single gene encoding pyruvate carbo

20 ithii +, plus the new eastern North American C. reinhardtii isolates, comprise one morphological spec

21 ommon unicellular ancestor of V. carteri and C. reinhardtii and that this gene was lost in the latter

22 region with several predicted V. carteri and C. reinhardtii proteins and that this region, the VARL d

23 mplex obtained from spinach chloroplasts and C. reinhardtii.

24 nce between cytochrome f of P. laminosum and C. reinhardtii (E(m7) = 297 and 370 mV, respectively).

25 l cyt bc(1) complex and the M. laminosus and C. reinhardtii cyt b(6)f complexes.

26 lesser degree between the higher plants and C. reinhardtii.

27 determined for both E. coli thioredoxin and C. reinhardtii thioredoxin h.

28 pendent signaling cascades operate in anoxic C. reinhardtii cells.

29 of the wide array of experimental approaches C. reinhardtii offers, Lechtreck and Witman determined t

30 ned elusive in eukaryotic microalgae such as C. reinhardtii.

31 tion pathway in the dark, especially because C. reinhardtii PFR1 was also able to allow H(2) evolutio

32 response is physiologically relevant because C. reinhardtii experiences these growth conditions routi

33 n the tetrads resulting from a cross between C. reinhardtii and C. smithii.

34 nach and pea thioredoxin f, -300 mV for both C. reinhardtii and spinach thioredoxin m, -320 mV for sp

35 nd/or ASQD in photosynthesis as conducted by C. reinhardtii, particularly under phosphate-limited con

36 nic forms of Se, are readily internalized by C. reinhardtii, but selenite is accumulated around ten t

37 at Sc hydroxo-complexes were internalized by C. reinhardtii.

38 dentify the molecular basis of the classical C. reinhardtii mutation ac17.

39 ST) evidence and annotation of the completed C. reinhardtii genome identified genes for each of the f

40 In conclusion, C. reinhardtii hydin is a CP protein required for flagel

41 Co-culturing C. reinhardtii with M. loti also results in reduction of

42 The deduced C. reinhardtii mature GTR amino acid sequence has more t

43 thway for Hyd1 expression in oxygen-depleted C. reinhardtii demonstrates the existence of multiple ox

44 gment length polymorphisms between divergent C. reinhardtii strains have been used to place each Dhc

45 ived sequences among nuclear genome data for C. reinhardtii, which also contrasts with the situation

46 nd frequency of the oscillatory dynamics for C. reinhardtii.

47 to the chloroplast stroma was estimated for C. reinhardtii cells grown under different conditions.

48 toxicity and adaptive response pathways for C. reinhardtii exposed to silver.

49 comparison of genomic Hind10 fragments from C. reinhardtii rs-3 and its wild-type progenitor CC-407

50 oxidant function, the nuclear VTE2 gene from C. reinhardtii was overexpressed in the npq1 lor1 double

51 dentical to the known HydA1 hydrogenase from C. reinhardtii.

52 ecificity and affinity as RB47 isolated from C. reinhardtii chloroplasts.

53 As isolated from C. reinhardtii flagella, IFT complex B can be further re

54 ty in the spectra for WT PS I particles from C. reinhardtii and Synechocystis sp. 6803 indicates that

55 For PS I particles from C. reinhardtii, substitution of HisA676 with serine has

56 00(+) formation, in both PS I particles from C. reinhardtii, the higher-frequency carbonyl band upshi

57 Evidently, docking of these proteins from C. reinhardtii is due to hydrophobic interaction, slight

58 and by nuclear transformation with VTE2 from C. reinhardtii, which resulted in 1.6-fold, 5-fold to 10

59 he previously identified SUMO conjugase gene C. reinhardtii ubiquitin-conjugating enzyme9 (CrUBC9) is

60 However, C. reinhardtii also possesses the pyruvate:ferredoxin ox

61 vealed a gene encoding a recently identified C. reinhardtii chloroplast carbonic anhydrase (CAH3).

62 In C. reinhardtii cells synchronized by light to dark cycle

63 In C. reinhardtii, low levels of a transcript encoding an o

64 In C. reinhardtii, the STA7 isoamylase gene is important fo

65 In C. reinhardtii, this mechanism is inducible and is prese

66 signal for acclimation to limiting CO(2) in C. reinhardtii is unidentified, and it is not known how

67 arteri but that a similar array is absent in C. reinhardtii.

68 a crucial part of high-light acclimation in C. reinhardtii.

69 IC70 minigene and measured accumulation, in C. reinhardtii, of transcripts from the endogenous gene

70 cular and nonvascular plant databases and in C. reinhardtii but absent from cyanobacterial genomes.

71 ot detection of CSRA and CSRB apoproteins in C. reinhardtii cells enabling assessment of the cellular

72 ER stress also triggered autophagy in C. reinhardtii based on the protein abundance, lipidatio

73 ne-folate cycle are also repressed by B12 in C. reinhardtii.

74 retinal-dependent photomotility behavior in C. reinhardtii.

75 rk provides insight into TAG biosynthesis in C. reinhardtii, and paves the way for engineering microa

76 cts with an antibody to lumen-directed CA in C. reinhardtii, and because it can be removed with 1 M C

77 tinct from the two mitochondrial beta-CAs in C. reinhardtii.

78 pyrenoid is required for a functional CCM in C. reinhardtii.

79 mponents of a high molecular mass complex in C. reinhardtii cells.

80 of at least one additional SUMO conjugase in C. reinhardtii, a conjugase tentatively identified as Cr

81 istinct and functional SUMO E2 conjugases in C. reinhardtii, with a clear division of labor between t

82 We predicted a large number of CREs in C. reinhardtii that are consistent with experimentally v

83 the first large-scale collection of CREs in C. reinhardtii to facilitate further experimental study

84 mplex integration patterns of plasmid DNA in C. reinhardtii nuclear transformants should be useful fo

85 ured airway epithelial cells, as was DRC2 in C. reinhardtii following deflagellation.

86 rafish embryos or mutation of TTC26/DYF13 in C. reinhardtii, produced short cilia with abnormal motil

87 h as much as approximately 10% efficiency in C. reinhardtii We demonstrate its use in transgene- and

88 cyanobacterial b(6)f, as well as eicosane in C. reinhardtii, are unique to the b(6)f complex.

89 ral dynamics of osmotic Ca(2+) elevations in C. reinhardtii suggest important mechanistic differences

90 Examination of this processing event in C. reinhardtii strains containing mutations within the c

91 y omega-3 fatty acid desaturase expressed in C. reinhardtii, and we discuss possible mechanisms of ho

92 When expressed in C. reinhardtii, we observed a differential metal-depende

93 known chloroplast ferredoxins (FDX1-FDX6) in C. reinhardtii, FDX1 and FDX2 were the most efficient el

94 ly regulated by the levels of PPO and FeC in C. reinhardtii.

95 the pyrenoid is the site of CO2 fixation in C. reinhardtii and other unicellular algae containing CO

96 ults suggest a mechanism for LD formation in C. reinhardtii involving chloroplast envelope membranes

97 to both Class-1 and Class-2 enzyme-forms in C. reinhardtii.

98 The actin-related gene in C. reinhardtii contains seven introns in the coding regi

99 reported conventional actin-encoding gene in C. reinhardtii.

100 he molecular cloning of the two Ppc genes in C. reinhardtii (CrPpc1, CrPpc2), each of which is transc

101 LciB and three related genes in C. reinhardtii compose a unique gene family that encode

102 nt high-level expression of foreign genes in C. reinhardtii, which has not previously been reliably a

103 l transcripts derived from the same genes in C. reinhardtii.

104 rom specific photosynthesis-related genes in C. reinhardtii.

105 We monitored the accumulation of GFP in C. reinhardtii chloroplasts transformed with the codon-o

106 s expression with the accumulation of GFP in C. reinhardtii transformed with a non-optimized GFP cass

107 Overexpression of GPD2 and GPD3 in C. reinhardtii gave distinct phenotypes.

108 photoreceptor for light induction of gsa in C. reinhardtii is not a carotenoid.

109 The FeC content is higher in C. reinhardtii cells growing in continuous light than in

110 -regulatory element (CRE) identification, in C. reinhardtii.

111 Calcium binding site IV in C. reinhardtii centrin was found to bind Ca2+ approximat

112 l to enforcing wild-type flagellar length in C. reinhardtii.

113 identification at the whole genome level in C. reinhardtii using a comparative genomics-based method

114 protein phosphorylation under high light in C. reinhardtii, known to fully induce the expression of

115 omycin define three unlinked nuclear loci in C. reinhardtii.

116 sed a chloroplast luciferase gene, luxCt, in C. reinhardtii chloroplasts under the control of the ATP

117 inorganic carbon-concentrating mechanism in C. reinhardtii and that genomic complementation can be a

118 gene family, which consists of 12 members in C. reinhardtii and 14 in V. carteri, has experienced a c

119 duced expression of Pcdp1 complex members in C. reinhardtii results in failure of the C1d central pai

120 of the transcriptome and mRNA metabolism in C. reinhardtii.

121 meostatic mechanism for copper metabolism in C. reinhardtii.

122 tal validation of several novel microRNAs in C. reinhardtii that were predicted by miRvial but missed

123 F35 is a nuclear mutation in C. reinhardtii that specifically affects translation of

124 vement of DNA from chloroplast to nucleus in C. reinhardtii, which may reflect the ultrastructure of

125 the transgene silencing that often occurs in C. reinhardtii, the FPs were expressed from the nuclear

126 ectronic structure of P700 and/or P700(+) in C. reinhardtii and Synechocystis sp. 6803.

127 Artificial microRNA silencing of PDAT in C. reinhardtii alters the membrane lipid composition, re

128 edoxin, is thus likely the partner of PFO in C. reinhardtii.

129 panying software tool and the predictions in C. reinhardtii are also made available through a Web-acc

130 yldiacylglycerol (ASQD), which is present in C. reinhardtii.

131 inglet oxygen-induced acclimation process in C. reinhardtii.

132 on of ATG8 and thus autophagy progression in C. reinhardtii.

133 atter gene seems to have its own promoter in C. reinhardtii.

134 Expression of the PsbS protein in C. reinhardtii has not been reported yet.

135 show that PsbS is a light-induced protein in C. reinhardtii, whose accumulation under high light is f

136 activation of non-photochemical quenching in C. reinhardtii, possibly by promoting conformational cha

137 together for coordinated gene regulation in C. reinhardtii.

138 vents in the global N starvation response in C. reinhardtii, starting within minutes with the upregul

139 uli, including a novel [Ca2+]cyt response in C. reinhardtii.

140 s associated with S deprivation responses in C. reinhardtii.

141 on factor-evolved from its ancestral role in C. reinhardtii as a mating-type specifier, to become a d

142 starch metabolism play an important role in C. reinhardtii H(2) photoproduction.

143 als that the miRNA-mediated RNA silencing in C. reinhardtii differs from that of higher plants and in

144 nsduction pathway controls organelle size in C. reinhardtii.

145 porally to synthesize diverse TAG species in C. reinhardtii.

146 d membranes occurs during iron starvation in C. reinhardtii.

147 rol of autophagy in response to ER stress in C. reinhardtii In close agreement, we also found that au

148 te that the rate of chlorophyll synthesis in C. reinhardtii is not directly controlled by the express

149 -mediated repression of protein synthesis in C. reinhardtii may involve alterations to the function/s

150 Functionality of the 5' UTRs was tested in C. reinhardtii chloroplasts using beta-glucuronidase rep

151 These data suggest that, in C. reinhardtii, (i) PHOT is involved in blue-light-media

152 Our results suggest that, in C. reinhardtii, miRNAs might be subject to relatively fa

153 tioned for mRNA stability and translation in C. reinhardtii chloroplasts while the more divergent C.

154 used to regulate chloroplast translation in C. reinhardtii.

155 andidates for distributive iron transport in C. reinhardtii.

156 SUMO-conjugating enzyme (SCE) (E2, Ubc9) in C. reinhardtii was shown to be functional in an Escheric

157 These tools are now being utilized in C. reinhardtii and in other algal species for the develo

158 9 kDa in extracts from anaerobically induced C. reinhardtii cells, strongly suggesting that HydA2 enc

159 1-tubulin gene and stably introduced it into C. reinhardtii cells.

160 arge-scale preparations and transformed into C. reinhardtii cells.

161 by importing mutant precursors into isolated C. reinhardtii chloroplasts.

162 ed may vary in photosynthetic organisms like C. reinhardtii from anoxia to high light to limitations

163 phage library was demonstrated by using live C. reinhardtii cells to pan for VH H clones with specifi

164 ing its codon usage to reflect that of major C. reinhardtii chloroplast-encoded proteins.

165 utionary distance between algae and mammals, C. reinhardtii ATPase 6 functioned in human cells, becau

166 investigated in carotenoid-deficient mutant C. reinhardtii cells.

167 The native C. reinhardtii-PSII WOC cycles less efficiently at all l

168 environment (G x E) variation in 18 natural C. reinhardtii accessions in 30 environments.

169 hing 38% (5884) of the 15,501 nonoverlapping C. reinhardtii genes.

170 shown to be critical for the acclimation of C. reinhardtii to S limitation.

171 ii Our results indicate that the activity of C. reinhardtii ATG4 is regulated by the formation of a s

172 eme inhibition was restored upon addition of C. reinhardtii soluble proteins.

173 Southern blot analysis of C. reinhardtii genomic DNA indicates that C. reinhardtii

174 lasticity of the photosynthetic apparatus of C. reinhardtii This alga is able to use various photoacc

175 w, using ODAs extracted from the axonemes of C. reinhardtii, that the C-terminal beta-propeller but n

176 ototrophic growth deficiency in strain B6 of C. reinhardtii.

177 tudying the biochemistry and cell biology of C. reinhardtii chloroplasts.

178 on-dense vacuoles or polyphosphate bodies of C. reinhardtii showed large amounts of phosphorus, magne

179 A possible role for Cah6 in the CCM of C. reinhardtii is proposed.

180 ; also named NAR1.2) and LCIB, in the CCM of C. reinhardtii.

181 nt results for deuterated wild-type cells of C. reinhardtii demonstrating that both radical pairs P70

182 ation of high light to dark-adapted cells of C. reinhardtii led to an increase in the amplitudes of 6

183 Therefore, in mixotrophically grown cells of C. reinhardtii, interpretations of the effects of enviro

184 ette and transformed into the chloroplast of C. reinhardtii and into E. coli.

185 ation in Escherichia coli by coexpression of C. reinhardtii HydEF and HydG and the HydA1 [FeFe] hydro

186 mentally measured steady-state Cd content of C. reinhardtii in the presence of low or high [Zn(2+)].

187 usly shown that when mixotrophic cultures of C. reinhardtii (which use both photosynthesis and mitoch

188 n the regulation of the sexual life cycle of C. reinhardtii, which is controlled by blue and red ligh

189 n important role in the sexual life cycle of C. reinhardtii: It controls the germination of the alga,

190 d on this we suggest that the development of C. reinhardtii as an industrial biotechnology platform c

191 n opportunity to expedite the development of C. reinhardtii as an industrial biotechnology platform,

192 e 251-residue extrinsic functional domain of C. reinhardtii cytochrome f was expressed in Escherichia

193 Further exploitation of C. reinhardtii as a model system to elucidate various mo

194 ed 309,278 raw EST sequencing trace files of C. reinhardtii and found that only 57% had cDNA termini

195 lustrate the marked metabolic flexibility of C. reinhardtii and contribute to the development of an i

196 CO2-concentrating mechanism or for growth of C. reinhardtii at limiting CO2 concentrations.

197 lify these regions from multiple isolates of C. reinhardtii.

198 PSI-LHCI-LHCII of C. reinhardtii is the largest PSI supercomplex isolated

199 he current status of genetic manipulation of C. reinhardtii for metabolic engineering.

200 ple changes in the nutrition and motility of C. reinhardtii.

201 he wild type (WT) and the HS(A676) mutant of C. reinhardtii indicates that the mutation primarily exe

202 isolation of a plasmid disruption mutant of C. reinhardtii, designated Deltasqd1, which lacks ASQD a

203 S I particles from a site-directed mutant of C. reinhardtii, in which the axial histidine ligand (His

204 Eight independently isolated mutants of C. reinhardtii that require high CO(2) for photoautotrop

205 A set of nuclear mutants of C. reinhardtii were identified that specifically lack tr

206 e compared with two site-directed mutants of C. reinhardtii, in which the spin-polarized signal on ei

207 observed in other PSII-deficient mutants of C. reinhardtii, suggesting a peripheral location of PSII

208 ropriate for the soil environmental niche of C. reinhardtii.

209 hemical properties of the homodimeric PFO of C. reinhardtii expressed in Escherichia coli.

210 Several of the large subunit proteins of C. reinhardtii have short extension or insertion sequenc

211 s, and thus should represent a wide range of C. reinhardtii antigens.

212 lly validated genome-scale reconstruction of C. reinhardtii metabolism that should serve as a useful

213 e utility of the GFPct gene as a reporter of C. reinhardtii chloroplast gene expression.

214 oid membrane proteins and in the response of C. reinhardtii to light and macronutrient stress.

215 show how FtsH is involved in the response of C. reinhardtii to macronutrient stress.

216 5, a protein that regulates the responses of C. reinhardtii to low-CO2 conditions.

217 Overall, the chloroplast ribosome of C. reinhardtii is similar to those of spinach chloroplas

218 gA gene, we analyzed the genome sequences of C. reinhardtii and V. carteri to identify additional gen

219 . eugametos) and five independent strains of C. reinhardtii.

220 ned the high-resolution crystal structure of C. reinhardtii ODA16 (CrODA16) and mapped the binding to

221 tii and determined the crystal structures of C. reinhardtii IFT70/52 and Tetrahymena IFT52/46 subcomp

222 tools currently being applied to studies of C. reinhardtii.

223 ectable marker for nuclear transformation of C. reinhardtii, composed of the coding sequence of the e

224 le markers for the genetic transformation of C. reinhardtii.

225 Moreover, we found that treatment of C. reinhardtii cells with norflurazon, an inhibitor of c

226 Treatment of C. reinhardtii cells with the ER stressors tunicamycin o

227 indicate that the electron-dense vacuoles of C. reinhardtii are very similar to acidocalcisomes with

228 The natural variation of C. reinhardtii accessions supports the hypothesis that,

229 with picosecond-fluorescence spectroscopy on C. reinhardtii cells that, although LHCs indeed detach f

230 s encoding the mouse homologues of the other C. reinhardtii C1d complex members are primarily express

231 in the internal thylakoid lumen of log phase C. reinhardtii.

232 gh TRP channels seem to be absent in plants, C. reinhardtii possesses genomic sequences encoding TRP

233 ansition from state 1 to state 2 can protect C. reinhardtii in high-light conditions and how this dif

234 We have characterized recombinant C. reinhardtii VTC2 as an active GDP-L-galactose phospho

235 with two cell types, one of which resembles C. reinhardtii cytologically but is terminally different

236 When acid shocked, C. reinhardtii excises its flagella, rapidly and coordin

237 R sequences from four Chlamydomonas species (C. reinhardtii, C. incerta, C. moewusii and C. eugametos

238 The standard C. reinhardtii strains, plus C. smithii +, plus the new

239 RNA-Seq analysis of iron-starved C. reinhardtii cells revealed notable changes in many tr

240 We demonstrate that C. reinhardtii chloroplasts transformed with the GFPct c

241 We hypothesized that C. reinhardtii ATPase 6 is nucleus encoded and identifie

242 Physiological analyses indicate that C. reinhardtii possesses multiple Ci transport systems r

243 These results indicate that C. reinhardtii VTC2, like its plant homologs, is a highl

244 , systems-level investigation indicated that C. reinhardtii cells sense and respond on a large scale

245 of C. reinhardtii genomic DNA indicates that C. reinhardtii has only one gtr gene.

246 Phylogenetic analysis indicates that C. reinhardtii PPO and FeC are most closely related to p

247 Chlamydomonas reinhardtii, and we show that C. reinhardtii PGK1 (CrPGK1) activity is inhibited by th

248 Our results suggest that C. reinhardtii employs a clostridial type H(2) productio

249 Immunoblotting results suggest that C. reinhardtii PPO and FeC are targeted exclusively to t

250 The C. reinhardtii cofactor proteins alone were all unable t

251 The C. reinhardtii LD fraction contained minimal proteins wi

252 of spinach chloroplast and E. coli, but the C. reinhardtii ribosome has proteins associated with the

253 firming that the two cDNAs indeed encode the C. reinhardtii chloroplast envelope carrier protein LIP-

254 We also examined the C. reinhardtii nuclear methylation map with base-level r

255 Moreover, the data generated for the C. reinhardtii double mutant clearly showed a complement

256 The amino acid sequences derived from the C. reinhardtii clones have extensive homology with GS en

257 oplast was due to the codon bias seen in the C. reinhardtii chloroplast genome.

258 els of recombinant protein expression in the C. reinhardtii chloroplast was due to the codon bias see

259 In this report we study the changes in the C. reinhardtii cyclophilin transcript and protein levels

260 abidopsis thaliana which is conserved in the C. reinhardtii enzyme, indicated localization in the pla

261 In the C. reinhardtii genome, the HydEF gene is adjacent to ano

262 with two Rub1 genes and one Ufm1 gene in the C. reinhardtii genome.

263 last PSRP-7 and EF-Ts sequences found in the C. reinhardtii genome.

264 at a rate of about one every 17.7 kb in the C. reinhardtii genome.

265 All four loci were highly polymorphic in the C. reinhardtii isolates.

266 on by tunicamycin was more pronounced in the C. reinhardtii sor1 mutant, which shows increased expres

267 currently used to modify and interrogate the C. reinhardtii nuclear genome and explore several techno

268 The flanking sequence matched the C. reinhardtii Rca cDNA sequence previously deposited in

269 The amino acid sequence of the C. reinhardtii ATP sulfurylase, derived from the nucleot

270 lowed brilliant and specific staining of the C. reinhardtii cell wall and analysis of cell-wall genes

271 which may reflect the ultrastructure of the C. reinhardtii cell.

272 Lipid profiling of the C. reinhardtii cells revealed that they degraded FAs but

273 small subunit protein to the pyrenoid of the C. reinhardtii chloroplast in cells maintained under amb

274 lthough paradoxically, the GC content of the C. reinhardtii genome is very high.

275 sduction effector for light induction of the C. reinhardtii gsa gene is a flavoprotein.

276 Moreover, co-expression of the C. reinhardtii HydEF, HydG, and HydA1 genes in Escherich

277 epigenetic programs during key stages of the C. reinhardtii life cycle.

278 P cassette (GFPct), under the control of the C. reinhardtii rbcL 5'- and 3'-UTRs.

279 Sequencing of the C. reinhardtii Rca gene revealed that it contains 10 exo

280 roteins were identified as components of the C. reinhardtii ribosome.

281 of the ALS promoter with the promoter of the C. reinhardtii Rubisco small subunit gene (RbcS2) permit

282 The GP1 protein, an HRGP of the C. reinhardtii wall, is shown to adopt a polyproline II

283 e betaC-plastoglobuli proteome resembles the C. reinhardtii eyespot and Arabidopsis (Arabidopsis thal

284 We conclude that the C. reinhardtii Flv participates in a Mehler-like reducti

285 , although developed for and tailored to the C. reinhardtii dataset, can be exploited by any eukaryot

286 NRT3.1 and AtNRT3.2, that are similar to the C. reinhardtii NAR2 gene.

287 Treating the C. reinhardtii wild type with mercuric ions, which were

288 essful complementation was achieved with the C. reinhardtii TLA2-CpFTSY gene, whose occurrence and fu

289 H H clones (designated B11 and H10) bound to C. reinhardtii with EC50 values </= 0.5 nm.

290 solated from the library show specificity to C. reinhardtii and lack of reactivity to antigens from f

291 ion of the chimeric gene using either of two C. reinhardtii chloroplast promoters and 5' and 3' RNA e

292 robically induced concomitantly with the two C. reinhardtii [Fe] hydrogenase genes, HydA1 and HydA2.

293 ition equivalent to Val-389 of the wild-type C. reinhardtii cDNA.

294 ses that accompany anaerobiosis in wild-type C. reinhardtii cells and a null mutant strain for the HY

295 respectively, was isolated from a wild-type C. reinhardtii library.

296 Transformation of wild-type C. reinhardtii with the mutant ALS gene produced no tran

297 When C. reinhardtii was transferred from the dark to very low

298 sults in decreased linear electron flow when C. reinhardtii is limited for either P or S.

299 tion of cilia, and other processes for which C. reinhardtii is a leading model system.

300 While C. reinhardtii is unicellular, V. carteri is multicellul