ライフサイエンスコーパス: C2 domain

1 osolic region of E-Syts (i.e., the number of C2 domains).

2 sidue ordered region C-terminal to the VPS34 C2 domain.

3 ids and PTEN phospho-tail for binding to the C2 domain.

4 domain inhibitor antibodies and the isolated C2 domain.

5 cular interaction between its C-tail and the C2 domain.

6 pitopes on opposing faces of the factor VIII C2 domain.

7 1 domain by replacing it with the homologous C2 domain.

8 antibody inhibitors against the factor VIII C2 domain.

9 otyrosine-binding site within its N-terminal C2 domain.

10 tivation of NF-kappaB by CC2D1A requires its C2 domain.

11 ontains 10 predicted Armadillo repeats and a C2 domain.

12 ovel site in the cationic beta-groove of the C2 domain.

13 also binds to the membrane through a cryptic C2 domain.

14 rm a Y-shaped complex, centered on the Vps34 C2 domain.

15 von Willebrand factor, and mediation by the C2 domain.

16 nd to several distinct surfaces on the FVIII-C2 domain.

17 branes with antiparallel orientations of the C2 domains.

18 ain, and two conserved tandem Ca(2+)-binding C2 domains.

19 tructures show close contacts between A1 and C2 domains.

20 8I) to better occupy a cavity between A1 and C2 domains.

21 phatidylcholine, in the presence of the syt1 C2 domains.

22 the protein or via mutagenesis of its tandem C2 domains.

23 tion is a derived feature limited to the PKC-C2 domains.

24 through its tandem, lectin-homology, C1 and C2 domains.

25 tate for Unc13 mediated by the tandem C1 and C2 domains.

26 (s)--and forskolin-stimulated activity of C1/C2 domains.

27 A (HA) are primarily directed to the A2 and C2 domains.

28 ded with peptides spanning FVIII-A2, C1, and C2 domains.

29 pes of factor VIII (fVIII) are in the A2 and C2 domains.

30 mbranes are tightly tethered by Ca(2+)-bound C2 domains.

31 develop antibodies against the fVIII A2 and C2 domains.

32 iated proteins that contain a tandem pair of C2 domains.

33 ature of the linker that connects its tandem C2 domains.

34 and alter the relative orientation of these C2-domains.

35 ansmembrane, linker, and two Ca(2+)-binding (C2) domains.

36 The C2 domain/3E6 FAB/G99 FAB ternary complex illustrates th

37 a classical calcium-dependent lipid binding C2 domain, a specific CAR signature is likely responsibl

38 ochondria by a mechanism that depends on its C2 domain, a unique Glu residue in its activation loop,

39 The C2-domain ABA-related (CAR) family of small proteins is

40 s are mediated through a 10-member family of C2-domain ABA-related (CAR) proteins in Arabidopsis thal

41 t a membrane-binding defective mutant of the C2 domain abrogated these properties.

42 n of Munc13-1, or of Munc13-1 with a mutated C2 domain all disrupted L-type channel clustering at gra

43 Furthermore, we demonstrated that the C2 domain alone is capable of targeting PLC to the membr

44 disrupted the interaction between the tandem C2 domains, altered the intrinsic affinity of syt-1 for

45 dylinositol 3-phosphate (PtdIns(3)P) via its C2 domain, an association that may be required for endos

46 ombined data show anillin contains a cryptic C2 domain and a Rho-binding domain.

47 3E6 and G99 bind independently to the fVIII C2 domain and can form a stable ternary complex.

48 containing 1A (CC2D1A) gene, which encodes a C2 domain and DM14 domain-containing protein, has been l

49 e nucleus by a mechanism that depends on the C2 domain and requires Src kinase activity.

50 es a truncated form of CC2D1A that lacks the C2 domain and three of the four DM14 domains, we show th

51 Multiple C2 domain and transmembrane region proteins (MCTPs) are

52 min in which the Ca(2+)-binding sites in the C2 domains and a basic cluster involved in membrane bind

53 ease similar C-terminal modules, bearing two C2 domains and a transmembrane domain.

54 lineage-specific expansions of Zizimin-type C2 domains and functionally linked CDC42/Rac GTPases occ

55 component to the interaction between ferlin C2 domains and lipid bilayers.

56 teins that bind the plasma membrane (PM) via C2 domains and transport lipids between them via SMP dom

57 Analysis of chimeric molecules, isolated C2 domains, and point mutants revealed that the C2B doma

58 attachment protein receptor), Synaptotagmin C2 domains, and the lipid bilayer in real time during th

59 ble fragment of syt1, which contains its two C2 domains, and the neuronal core soluble N-ethylmaleimi

60 synaptotagmin-1 is used instead of isolated C2 domains, and when liposomes containing a physiologica

61 Within the C2 domain, antibody epitope and kinetics are more import

62 ent conformations (all of its domains except C2 domain are visible).

63 domains of synaptotagmins, the Rasal tandem C2 domains are able to sense and induce membrane curvatu

64 malian cells and showed that while wild-type C2 domains are efficiently secreted, the mutant p.D871N

65 lution binding affinity does not change when C2 domains are linked.

66 Ca(2+)-responsive C2 domains are peripheral membrane modules that target t

67 Proteins containing C2 domains are the sensors for Ca(2+) and PI(4,5)P2 in a

68 C2 domains are widespread motifs that often serve as Ca(

69 and C2E domains and, to a lesser extent, the C2D domain are dispensable for dysferlin membrane repair

70 epitopes are on opposing sides of the fVIII C2 domain, are consistent with the solvent accessibility

71 The 3E6 epitope forms direct contacts to the C2 domain at 2 loops consisting of Glu2181-Ala2188 and T

72 ns four DM14 domains at the N terminus and a C2 domain at the C terminus.

73 his interaction was facilitated by a cryptic C2 domain at the extreme N terminus of Psd2p (C2-1) as w

74 tanding of Notch signalling by identifying a C2 domain at the N-terminus of Notch ligands, which has

75 n two calcium-dependent phospholipid binding C2 domains at the amino terminus and a VWA domain at the

76 CAPS dimer formation required its C2 domain based on mutation or deletion studies.

77 ariations depending on the properties of the C2 domain-bearing protein, shedding light to understand

78 common ancestor (LECA) contained at least 10 C2 domains belonging to 6 well-defined families.

79 ally, sedimentation assays suggest all seven C2 domains bind lipid membranes, and that Ca(2+) enhance

80 Usually C2 domains bind membrane lipids, but that of PLCgamma2 d

81 Both the phosphatase and C2 domains bind phosphatidylserine lipids, which likely

82 Their respective C2 domains bind via 2 pairs of hydrophobic amino acids a

83 Taken together, these data suggest that C2 domain binding to membranes is an important element i

84 ined in flexible top-loop sequences of their C2 domains--blocked the ability of these synaptotagmins

85 The C2 domains bracket a predicted SNARE-binding domain, but

86 near the Ca(2+)-binding surface loops of the C2 domain, but binding to phosphoinositide-containing ve

87 ase by virtue of Ca(2+)-binding to their two C2 domains, but their mechanisms of action remain unclea

88 mechanism of Ca(2+) and lipid binding to the C2 domain by NMR spectroscopy and x-ray crystallography.

89 ated the apparent synergy between the tandem C2 domains by altering the length and rigidity of the li

90 t containing a disulfide bond between A1 and C2 domains by mutating Arg-121 and Leu-2302 to Cys (R121

91 Here we examine the effect of the two C2 domains (C2A and C2B) of syt1 on membrane lipid order

92 Munc13-4 contains N- and C-terminal C2 domains (C2A and C2B) predicted to bind Ca(2+), but C

93 Syt1 contains two C2 domains (C2A and C2B) that are homologous in sequence

94 eractions mediated by its tandem cytoplasmic C2 domains (C2A and C2B).

95 ), is largely composed of two Ca(2+)-sensing C2 domains, C2A and C2B.

96 soluble fragment of syt1 containing its two C2 domains, C2A and C2B.

97 nd a fragment containing the synaptotagmin-1 C2 domains (C2AB) to membrane-anchored SNARE complex.

98 PLA(2)alpha truncated at the calcium-binding C2 domain (C2D).

99 The dual C2 domain Ca(2+)-binding mutant also enhanced spontaneou

100 This process requires otoferlin, a six C2-domain, Ca(2+)-binding transmembrane protein of synap

101 ring the plasma membrane-sensing role of the C2 domain, causes phorbol ester-triggered redistribution

102 requires the binding of Ca(2+) to two tandem C2 domains, CBD1 and CBD2, which are an integral part of

103 Calcium-binding C2 domain cells lost the ability of membrane translocati

104 n C2 domains of dysferlin as well as a multi-C2 domain construct.

105 luorescence measurements indicate that multi-C2 domain constructs of myoferlin, dysferlin, and otofer

106 variants in unrelated probands: WWC1 (WW and C2 domain containing 1), CELSR3 (Cadherin EGF LAG seven-

107 oforms (Syt2, Syt7, Syt9) as well as related C2 domain containing protein, Doc2B and extended Synapto

108 asts lacking one of these gene products, the C2-domain containing protein, SMURF1, are deficient in t

109 Mutation of the coiled-coil and C2 domain-containing 1A (CC2D1A) gene, which encodes a C

110 Mutations in otoferlin, a C2 domain-containing ferlin family protein, cause non-sy

111 We have recently demonstrated that a novel C2 domain-containing protein kinase, EhC2PK is involved

112 Several C2 domain-containing proteins are known to regulate Ca(2

113 by screening all ( approximately 139) human C2 domain-containing proteins by RNA interference in neu

114 The mammalian ferlins are calcium-sensing, C2 domain-containing proteins involved in vesicle traffi

115 Several C2 domain-containing proteins, such as protein kinase C

116 hat the C1 domain, in addition to the A2 and C2 domains, contributes significantly to the humoral ant

117 xplore the molecular mechanisms by which the C2 domain controls the initial step in the activation of

118 t participate in such tethering function via C2 domain-dependent interactions with the PM that requir

119 We constructed a FVIII variant lacking the C2 domain (designated DeltaC2) to characterize the contr

120 We found that mutations of aspartates in the C2 domains did not alter plasma membrane localization bu

121 Upon activation by Ca(2+), the PKCalpha C2 domain directs the kinase protein to the plasma membr

122 senses Ca(2+); moreover, the tethered tandem C2 domains display properties distinct from the isolated

123 Yao et al. now report that double C2 domain (Doc2) proteins function as high-affinity Ca(2

124 ns within the soluble calcium-binding double C2 domain (Doc2)-like protein family to selectively redu

125 membrane docking geometries of the PKCalpha C2 domain docked to (i) PS alone and (ii) both PS and PI

126 plex suggests that antibody 3E6 recognizes a C2 domain epitope consisting of the Arg(2209)-Ser(2216)

127 no detectable internal rearrangement of its C2 domains, even as it rapidly inserts into the bilayer.

128 In particular, the soluble C2-domain factor Doc2b plays a key role in GLUT4 vesicle

129 ations that disrupted Ca(2+) binding to both C2 domains failed to rescue evoked release, but supporte

130 C1 domains, indicating that the A1, A2, and C2 domains fold independently into antigenically intact

131 in constructs, each lacking one of the seven C2 domains, for their ability to localize to the plasma

132 In wild-type p110alpha, N345 in the C2 domain forms hydrogen bonds with D560 and N564 in the

133 We demonstrated previously that the C2 domain from PKCalpha (C2alpha) binds Pb(2+) with high

134 C2 domain from protein kinase Calpha (C2alpha) was chose

135 ce that Munc13-4 with its two Ca(2+)-binding C2 domains functions as a Ca(2+) sensor for SG exocytosi

136 ough located distant to the active site, the C2 domain greatly enhances catalytic turnover.

137 additional regulatory protein, Doc2b (double C2 domain), has recently been implicated in exocytosis f

138 For Syt1, the two Ca(2+)-saturated C2 domains have similar affinities for membranes lacking

139 We prepared mutants in which the C2 domain hydrophobic amino acid pairs were changed to t

140 e structures of the loops at the apex of the C2 domain implicated in membrane recognition and Jagged1

141 domains, in addition to the critical role of C2 domain in ASK1 activity, are important for modulating

142 rystallographic structure of the factor VIII C2 domain in complex with 2 antibodies that illuminates

143 These data underscore the key role of the C2 domain in driving conventional PKC isozymes to the pl

144 g proteins EHD1 and EHD2 and that the second C2 domain in Fer1L5 mediates this interaction.

145 erve clustering of anionic lipids around the C2 domain in preference to the phosphatase domain, sugge

146 eate the unique and shared functions of each C2 domain in regulation of synaptic vesicle fusion.

147 inding protein (SMP) domain followed by five C2 domains in E-Syt1 and three C2 domains in E-Syt2/3.

148 lowed by five C2 domains in E-Syt1 and three C2 domains in E-Syt2/3.

149 We expressed wild-type and mutant alpha2(VI) C2 domains in mammalian cells and showed that while wild

150 shown that EHD proteins bind directly to the C2 domains in myoferlin, a protein that regulates myobla

151 be a key determinant of the functions of Syt C2 domains in neurotransmitter release.

152 ins including those respectively typified by C2 domains in the Aida (axin interactor, dorsalization a

153 , we identified several distinct families of C2 domains including those respectively typified by C2 d

154 motif resembling that of membrane-targeting C2 domains, including a bound calcium ion.

155 opological similarity to an integral fold of C2 domains, including a putative basic binding pocket.

156 Similarly, mAb ESH4 against the C2 domain, inhibited >90% of platelet-dependent fVIII ac

157 lonal antibody (mAb) ESH8, against the fVIII C2 domain, inhibited binding of fVIII to SF and platelet

158 ctions between two different classes of anti-C2 domain inhibitor antibodies and the isolated C2 domai

159 UE domains and that its association with the C2 domain inhibits PtdIns(3)P binding.

160 asurements indicate that all seven dysferlin C2 domains interact with Ca(2+) with a wide range of bin

161 he absence of calcium, consistent with intra-C2 domain interactions forming a "closed" tertiary struc

162 opy, we found that the synergy between these C2 domains involved intra-molecular interactions between

163 The factor VIII C2 domain is a highly immunogenic domain, whereby inhibi

164 The C2 domain is a useful model to understand these events b

165 Whereas the FVIII C2 domain is believed to anchor FVIIIa to the phospholip

166 The membrane-binding activity of the C2 domain is functionally equivalent to the myristoylati

167 over effects observed when the extracellular C2 domain is included.

168 the phosphatase domain, suggesting that the C2 domain is involved in nonspecific interactions with n

169 s unknown if Ca(2+) interactions with either C2 domain is required for suppression of asynchronous re

170 indicate that cooperation between the C1 and C2 domains is necessary for full activity of the factor

171 ion to Ca2+ influx, but Ca2+ binding by both C2 domains is required to flip the electrostatic switch

172 rol in Arabidopsis, demonstrating that these C2 domains may be cooperative to mediate FTIP1 function

173 rt, we demonstrate that calcium releases the C2 domain-mediated auto-inhibition in both Nedd4-1 and N

174 nally, previously described Ca(2+)-dependent C2 domain-mediated autoinhibition of Nedd4-2 is not obse

175 We conclude that CAPS functions as a C2 domain-mediated dimer in regulated vesicle exocytosis

176 Following this C2 domain-mediated membrane recruitment, the C1 domain b

177 Ferlins are large multi-C2 domain membrane proteins involved in membrane fusion

178 -mediated membrane translocation through the C2 domain might be an activation mechanism of Nedd4 in v

179 Expression of the PTEN C2 domain mimicked effects of full-length PTEN but a mem

180 and analyzed Pten knock-in mice harboring a C2 domain missense mutation at phenylalanine 341 (Pten(F

181 dynamic folding options; 3) C-terminal four-C2 domain module; and 4) calpain-cleaved mini-dysferlinC

182 ction of the SYT tubes shows that one of the C2 domains (most likely C2B, based on its biochemical pr

183 C2 domain mutants (N345K and C420R) also mimic these eve

184 Moreover, C2 domain mutations or deletions resulted in a loss of C

185 ike protein MbSrc4, contains a lipid-binding C2 domain N-terminal to the conserved SH3-SH2-kinase dom

186 While two other distinct families of C2 domains, namely those in PI3K-C2 and PTEN-C2 are also

187 o alternative splicing to include DBL/PH and C2 domains not present in invertebrate Itsn proteins.

188 ween otoferlin C2F domain and intramolecular C2 domains occurred in the absence of calcium, consisten

189 ry complex, showing an angle centered at the C2 domain of approximately 130 degrees .

190 The C2 domain of fVIII is a significant antigenic target of

191 ssion of the II-III loop of the channel, the C2 domain of Munc13-1, or of Munc13-1 with a mutated C2

192 sine-independent, and GRB10 SH2 can bind the C2 domain of NEDD4 and the kinase domain of IGF1R simult

193 The C2 domain of NEDD4-1 is required for its interaction wit

194 The Tyr(P) peptide also binds the C2 domain of PKCdelta tightly, but no enzyme activation

195 Collectively, these studies establish the C2 domain of PKCtheta as a Tyr(P)-binding domain and sug

196 taining peptide derived from CDCP1 binds the C2 domain of PKCtheta with high affinity and activates t

197 The identified motifs are located in the C2 domain of plant PLCs and are not found in any other p

198 Overall, these data suggest that the C2 domain of PLC plays a vital role in calcium signallin

199 ex is controlled by the weakly characterized C2 domain of PLCgamma2.

200 lly, it is the first mutation located in the C2 domain of PLCZ1, a domain involved in targeting prote

201 ltiscale metal-ion-dependent dynamics of the C2 domain of protein kinase Calpha (C2alpha) using NMR s

202 e used to determine the configuration of the C2 domain of protein kinase Calpha (PKCalpha-C2) bound t

203 (C2-1) as well the previously characterized C2 domain of Psd2p (C2-2).

204 tB2p/Pdr17p; a PtdIns 4-kinase, Stt4p; and a C2 domain of Psd2p.

205 We demonstrate that the non-catalytic C2 domain of PTEN specifically binds PI(3)P through the

206 been shown that GRB10 can interact with the C2 domain of the E3 ubiquitin ligase NEDD4 through its S

207 The discoidin C2 domains of coagulation factors V and VIII are known t

208 and membrane binding properties of all seven C2 domains of dysferlin as well as a multi-C2 domain con

209 Of the five C2 domains of E-Syt1, only C2A and C2C contain Ca(2+)-bi

210 e (EGF) domains 8 and 12 engage the EGF3 and C2 domains of Jag1, respectively, and different Notch1 d

211 m coordinating residues in each of the three C2 domains of MCTP.

212 roteins, we demonstrate that five of the six C2 domains of otoferlin sense calcium with apparent diss

213 fusion assay, we found that five of the six C2 domains of otoferlin stimulate membrane fusion in a c

214 we investigate the effects of binding of the C2 domains of otoferlin, dysferlin, and myoferlin on the

215 an acceptor membrane complex containing the C2 domains of Psd2p, PstB2p, and Pbi1p that ligate to Sc

216 Ca-dependent interaction between the tandem C2 domains of Rasal and lipids of the membrane is also r

217 e of interfacial residues between the tandem C2 domains of synaptotagmin (syt)-1, a Ca(2+) sensor for

218 Finally we show, that similar to the C2 domains of synaptotagmins, the Rasal tandem C2 domain

219 These results suggest that the tandem C2 domains of Syt 1 play independent roles in neurotrans

220 hat physical interactions between the tandem C2 domains of syt-1 contribute to excitation-secretion c

221 ulated by Syt1 multimerization and that both C2 domains of Syt1 are uniquely required for modulating

222 Notably, either of the C2 domains of syt1, C2A or C2B, was able to function as

223 The two C2 domains of Syt1, which may mediate fusion by bridging

224 s used to estimate distances between the two C2 domains of syt1.

225 The tandem C2-domains of synaptotagmin 1 (syt) function as Ca(2+)-b

226 ly interacted with the lipid-binding domain (C2 domain) of PTEN and sequestered it in the nucleus.

227 Our identification of novel C2 domains offers new insights into interaction between

228 suggest that the effects of each subsequent C2 domain on lipid ordering appear to be additive.

229 We further analyze in vivo effects of three C2 domains on the regulatory role of MCTP1 (FTIP1) in fl

230 (PIP2) complexes, revealing how Rabphilin-3A C2 domains operate in cooperation with PIP2/Ca(2+) and S

231 Syt 1 transgenes containing only individual C2 domains, or dual C2A-C2A or C2B-C2B chimeras, failed

232 Beyond membrane insertion by the C2 domains, other requirements for Synaptotagmin activit

233 nase Calpha (PKCalpha) possesses a conserved C2 domain (PKCalpha C2 domain) that acts as a Ca(2+)-reg

234 in VWF binding, and suggest that the A3 and C2 domains play ancillary roles in this interaction.

235 pholipid-binding modules such as PH, C1, and C2 domains play crucial roles in location-dependent regu

236 ammalian Src substrates, suggesting that the C2 domain plays a specific role in M. brevicollis signal

237 efficiently drove exocytosis when its tandem C2-domains pointed in the same direction.

238 is involved in ring formation, and the other C2 domain points radially outward.

239 ns and antibody binding to human A2, C1, and C2 domains presented as human serum albumin (HSA) fusion

240 In contrast, antibody G99 recognizes the C2 domain primarily through the Pro(2221)-Trp(2229) loop

241 Monoclonal antibodies against the C2 domain produced different relative impairment of muta

242 on blocks Cdc15 binding to paxillin Pxl1 and C2 domain protein Fic1 and enhances Cdc15 dynamics.

243 Otoferlin is a large multi-C2 domain protein proposed to act as a calcium sensor th

244 Pxl1 and Fic1, a previously uncharacterized C2 domain protein, add structural integrity to the contr

245 Double C2-domain protein (Doc2) is a Ca(2+)-binding protein imp

246 ns Merlin and Expanded (Ex), and the WW- and C2-domain protein Kibra.

247 that block PHP we identified mctp (Multiple C2 Domain Proteins with Two Transmembrane Regions).

248 eral and conserved structural aspect of many C2 domain proteins, including Synaptotagmins.

249 and beta (Doc2alpha and Doc2beta) are tandem C2-domain proteins proposed to function as Ca(2+) sensor

250 Most studies have concentrated on C2 domains prototyped by those in protein kinase C (PKC-

251 in primarily through its C1 domain, with the C2 domain providing a secondary attachment site.

252 Collectively, these studies identify a C2 domain-pTyr(313) docking interaction that controls AT

253 stabilize an interface between the p110alpha C2 domain (residue N345) and the p85 iSH2 domain (residu

254 e changes in the relative disposition of the C2-domains result from changing the length of the poly-p

255 EF for Rac1), and show that DHR-1 utilizes a C2 domain scaffold and surface loops to create a basic p

256 Here we demonstrate that each of its C2 domains senses Ca(2+); moreover, the tethered tandem

257 ue structure of dysferlin, with seven tandem C2 domains separated by linkers, suggests dysferlin may

258 Disruption of Ca(2+) binding to the PKCbeta C2 domain specifically prevents PTP without impairing ot

259 notype combined with similar findings in the C2 domain stress the importance of inhibitor properties

260 ure of evolutionary relationships within the C2 domain superfamily is lacking.

261 ting a calcium-mediated stabilization of the C2 domain.t-SNARE complex.

262 Ca(2+)-dependent conserved-region 2 (C2) domains target their host signaling proteins to anio

263 Syt1 contains two cytoplasmic C2 domains, termed C2A and C2B, which coordinate Ca(2+)

264 hinge region and its phosphotyrosine-binding C2 domain that controls PKCdelta's enzymology indirectly

265 eakest affinity site triggers changes in the C2 domain that facilitate its interaction with lipid mem

266 nvestigated the properties of its N-terminal C2 domain that functions as an autoinhibitory domain.

267 to identify the conserved amino acids of the C2 domain that regulate the targeting of PLCZ1 and its s

268 delta structure reveals an unexpected second C2 domain that was previously unrecognized from sequence

269 membrane domain, two DysF domains, and seven C2 domains that mediate lipid- and protein-binding inter

270 13 and Tctex-1 (dynein light chain), and two C2-domains that bind to phospholipids, Ca(2+) and SNAREs

271 a) possesses a conserved C2 domain (PKCalpha C2 domain) that acts as a Ca(2+)-regulated membrane targ

272 in neurotransmitter release through its two C2 domains (the C2A and C2B domain).

273 gions are likely to be shared by majority of C2 domains, the actual constellation of lipid-binding re

274 Synaptotagmins bind Ca(2+) via two C2 domains, the C2A and C2B domains.

275 hrough binding of high local Ca(2+) to their C2 domains, the proteins that sense smaller global Ca(2+

276 hat are able to inhibit binding of the FVIII C2 domain to a model membrane by application of a combin

277 Comparison of the CAPS C2 domain to a structurally defined Munc13-1 C2A domain

278 he major driving force in the binding of the C2 domain to anionic membranes, whereas electrostatic in

279 tion by promoting anchoring of the PLCgamma2 C2 domain to phospho-SLP65.

280 Calcium disrupts binding of the C2 domain to the HECT domain.

281 y high Ca(2+) concentrations, binding of the C2 domain to the target lipid phosphatidylserine (PS) is

282 rfacial binding of mouse cPLA(2)beta and its C2 domain to vesicles.

283 sozymes is initiated by the binding of their C2 domains to membranes in response to elevations in int

284 led a correlation between the ability of the C2 domains to penetrate membranes in response to Ca(2+)

285 ral explanation for the ability of different C2 domains to pull plasma and vesicle membranes close to

286 differentially regulate binding of otoferlin C2 domains to target SNARE (t-SNARE) proteins and phosph

287 r Synaptotagmin binds Ca(2+) through its two C2 domains to trigger membrane interactions.

288 Otoferlin, a six-C2 domain transmembrane protein linked to deafness in hu

289 Structurally, SMURF1 consists of a C2 domain, two WW domain repeats, and a catalytic HECT d

290 s from a change in the hydrophobicity of the C2 domain upon Ca(2)(+) binding.

291 yses of intrinsic Ca(2+)-binding to the Syt7 C2 domains using isothermal titration calorimetry, did n

292 We study the diffusion of membrane-targeting C2 domains using single-molecule tracking in supported l

293 ersely, an RCP mutant lacking the PA-binding C2 domain was not capable of being tethered at pseudopod

294 alues in the tens of micromolar, whereas the C2D domain was least sensitive, with a near millimolar K

295 Finally, transfection of the PLA(2)IValpha C2 domain (which is directly involved in PLA(2)IValpha m

296 s to the kinase domain of PIPKIgamma via its C2 domain while Lysine 255 in PIPKIgamma acts as the maj

297 Direct binding measurements titrating the C2 domain with PIP(2) in lipid bilayers yield a 1:1 stoi

298 t step in this process is interaction of its C2 domain with target cell membranes, which is a calcium

299 f its N-terminal immunoglobulin (Ig)-like C0-C2 domains with actin and/or myosin.

300 soforms containing common C-terminal PDZ and C2 domains with homology to vertebrate active zone prote