ライフサイエンスコーパス: CALI

1 CALI did not negatively impact survival.

2 CALI do not negatively impact long-term prognosis, but t

3 CALI of MAG permitted significant regrowth of retinal ax

4 CALI of MII reduced neurite outgrowth and growth cone ar

5 CALI of myosin-V in growth cones of chick dorsal root ga

6 CALI of purified chick brain myosin-V absorbed onto nitr

7 CALI provides an approach to inactivate in vivo function

8 CALI worsen the short-term outcomes of liver resection,

9 EGFP has been used effectively as a CALI chromophore, and its cotranslational attachment to

10 r concentrations of protein, turbidity after CALI increased significantly indicating cross-linking of

11 All specimens were reviewed to assess CALI, TRG, and micrometastases.

12 ReAsH-based CALI is genetically targeted, requires no antibodies or

13 We now demonstrate CALI of connexin43 (Cx43) and alpha1C L-type calcium cha

14 At E12, CALI of ephrin-A5 did not affect the extent of axon outg

15 re bleaching and those that escape to effect CALI of proximate proteins.

16 Performing EGFP-CALI experiments in deficient cells rescued with functio

17 A potential drawback to EGFP-CALI is that the CALI phenotype can be obscured by the e

18 nglet oxygen respectively inhibit or enhance CALI.

19 n mediated by fluorescent protein CALI ([FP]-CALI), the activities of purified glutathione-S-transfer

20 Here, we generalize CALI so that it can be applied to a wider range of tasks

21 nder (FlAsH) results in significantly higher CALI efficiency than any of the fluorescent proteins (XF

22 Chromophore-assisted laser inactivation (CALI) is a light-mediated technique that offers precise

23 Chromophore assisted laser inactivation (CALI) is a technique that uses irradiation of chromophor

24 sed chromophore-assisted laser inactivation (CALI) to generate acute loss of ephrin-A5 function in lo

25 ted chromophore-assisted laser inactivation (CALI) to instantly and specifically inactivate it.

26 ing chromophore-assisted laser inactivation (CALI).

27 ing chromophore-assisted laser inactivation (CALI).

28 by chromophore-assisted laser inactivation (CALI).

29 to chromaphore-assisted light inactivation (CALI) , which resulted in the unexpected dissipation of

30 uct chromophore assisted light inactivation (CALI) of synaptic proteins.

31 Chromophore-assisted light inactivation (CALI) offers the only method capable of modulating speci

32 zes chromophore-assisted light inactivation (CALI) to inactivate presynaptic neurotransmitter release

33 Chromophore-assisted light inactivation (CALI) uses photochemically generated, reactive oxygen sp

34 Chromophore-Assisted Light Inactivation (CALI) using genetically-encoded photosensitizers provide

35 as chromophore-assisted light inactivation (CALI) with fluorescein derivatives, have been limited by

36 s as well as the removal of oxygen inhibited CALI, indicating the involvement of a reactive oxygen sp

37 pact of chemotherapy-related liver injuries (CALI), pathological tumor regression grade (TRG), and mi

38 ed green fluorescent protein (EGFP) mediated CALI has been used to inactivate EGFP-fusion proteins in

39 In conclusion, miniSOG-mediated CALI is a novel genetic platform for acute inactivation

40 ReAsH-mediated CALI acts largely via singlet oxygen because quenchers o

41 Micro-CALI of L1 causes neurite retraction after a 10 min lag

42 Micro-CALI of M1c reduced retrograde bead flow by 76%, whereas

43 Micro-CALI of MII caused a rapid reduction in local lamellipod

44 Micro-CALI of radixin targeted to the middle of the leading ed

45 Episodes of asymmetric micro-CALI of myosin 1c (or myosin 1c and V together) caused s

46 measured trajectories from asymmetric micro-CALI of myosin 1c-treated and untreated growth cones to

47 contribution to turning by asymmetric micro-CALI of myosin isoforms that causes localized lamellipod

48 Functional ablation of ezrin by micro-CALI (chromophore-assisted laser inactivation) blocked p

49 symmetric inactivation of myosin 1c by micro-CALI.

50 In contrast, micro-CALI of NCAM-180 causes rapid growth cone retraction but

51 romophore-assisted laser inactivation (micro-CALI) of radixin in growth cones causes a 30% reduction

52 romophore-assisted laser inactivation (micro-CALI) of these proteins to perturb their functions at pr

53 In contrast, repeated micro-CALI of myosin V or irradiation without added antibody d

54 e behavior of growth cones after these micro-CALI treatments resemble the drug-induced perturbation o

55 This is opposite to micro-CALI of M1c, which caused an increase in lamellipodial p

56 d retrograde bead flow by 76%, whereas micro-CALI of MII or the MIIB isoform did not.

57 illumination to examine the effectiveness of CALI targeted to kinesin.

58 Finally, we apply this implementation of CALI to an in vitro system of motor proteins and microtu

59 In contrast, labeling of CALI of MAG-treated crushed optic nerve showed significa

60 inactivation mediated by fluorescent protein CALI ([FP]-CALI), the activities of purified glutathione

61 luorescein, comparing them with the standard CALI dye, malachite green; and we study the relative eff

62 In this system, CALI can effectively perturb local structure formation b

63 We demonstrate that CALI of EGFP-CapZbeta increases uncapped actin filaments

64 We also demonstrate that CALI of EGFP-Mena in Mena/VASP-deficient cells stabilize

65 We show that CALI can destroy kinesin activity in at least two ways:

66 Specifically, we show that CALI can work with a genetically inserted epitope tag; w

67 potential drawback to EGFP-CALI is that the CALI phenotype can be obscured by the endogenous, unlabe

68 ferent FP mutants fused to GST vary in their CALI efficiency in the order enhanced green fluorescent

69 ition to globally inhibiting actin turnover, CALI of cofilin generated several profound effects on th

70 e this technique Inhibition of Synapses with CALI (InSynC).