ライフサイエンスコーパス: CDV

1 CDV (Km = 2.10 +/- 0.18 mM and Vmax = 1.10 +/- 0.05 micr

2 CDV is typically associated with domestic dogs, but litt

3 CDV treatment resulted in complete resolution of clinica

4 CDV was associated with improved clinical status, viral

5 CDV was discontinued after 7 weeks secondary to transien

6 Group 2 was administered 1% CDV twice a day for 3 days plus 1% Pred Forte four times

7 The metabolism of [3H]CDV in mock- and cytomegalovirus-infected cells was exam

8 ided into two topical treatment groups: 0.5% CDV and PBS control.

9 Topical 0.5% CDV treatment demonstrated significant antiviral inhibit

10 city of the wild-type CDV isolate 5804Han89 (CDV(5804)), the small- and large-plaque-forming variants

11 A CDV unable to recognize the signaling lymphocytic activa

12 d not alter the replication of the three Ad5 CDV variants on the rabbit eye.

13 Comparison with the ferret-adapted CDV(5804P) and the prototypic wild-type CDV(R252) showed

14 asis, than is intraperitoneally administered CDV.

15 patients with classical KS were administered CDV (5 mg/kg/dose) weekly for 2 weeks and then every oth

16 iant panda was previously vaccinated against CDV.

17 bivalent rabies virus-based vaccine against CDV induces protective immune responses against both pat

18 The coding regions of the H proteins of all CDV strains and MV(Edm) were introduced into the CDV and

19 ncomitant treatment with both Pred Forte and CDV significantly reversed the antiviral inhibitory acti

20 ntracellular levels of CDV monophosphate and CDV diphosphate increased approximately 20- and 8-fold,

21 DV vaccine strain Onderstepoort (CDV(OS) and CDV(OL), respectively), and the MV vaccine strain Edmons

22 by IFIT1, the translations of PIV3, SeV, and CDV mRNAs were not.

23 han did ferrets immunized with an attenuated CDV vaccine (0.46 +/- 0.59; n = 7) or the recombinant ve

24 ight loss, and both the NYVAC and attenuated CDV vaccines protected against the development of some c

25 profile and high stability, live-attenuated CDV vaccines can retain residual virulence in highly sus

26 accines are inactivated, the live-attenuated CDV vaccines retain residual virulence for highly suscep

27 Striking similarities between CDV infection of ferrets and human immunodeficiency viru

28 Cadicivirus (CDV) is unique amongst picornaviruses in having a dicist

29 We have tested candidate CDV vaccines incorporating the fusion (F) and hemaggluti

30 e kinase (EC 2.7.4.6) were found to catalyze CDV diphosphate synthesis from CDV monophosphate, wherea

31 eurovirulent Snyder Hill (SH) strain of CDV (CDV(SH)) and show that this virus rapidly circumvents th

32 Cidofovir (CDV) given by parenteral injection has been shown to pro

33 Cidofovir (CDV), a broad spectrum anti-DNA viral agent, has previou

34 Group 1 was administered 1% cidofovir (CDV) twice a day for 3 days plus comfort tears four time

35 conducted to test the activity of cidofovir (CDV), a drug with in vitro activity against Kaposi sarco

36 ve ether lipid ester analogues of cidofovir (CDV)--hexadecyloxypropyl-CDV (HDP-CDV) and octadecyloxye

37 he acyclic nucleoside phosphonate cidofovir (CDV) and its closely related analogue (S)-9-(3-hydroxy-2

38 the antiviral resistance of three cidofovir (CDV)-resistant variants of adenovirus type 5 (Ad5) and t

39 We used cidofovir (CDV), a nucleotide-analogue KSHV DNA polymerase inhibito

40 We report our experience with cidofovir (CDV) for treatment of ADV infection in 57 HSCT patients,

41 Cidofovir [CDV; (S)-1-(3-hydroxy-2-phosphonomethoxyethyl)cytosine]

42 main results are (i) in some circumstances, CDV may not distinguish well between a selected locus an

43 HDP-cyclic CDV, under simulated physiologic conditions, slowly conv

44 of a single intravitreal dose of HDP-cyclic-CDV to prevent viral retinitis for up to 68 days in a ra

45 s study, approximately 35% of the HDP-cyclic-CDV was converted to HDP-CDV.

46 HDP-cyclic-CDV was suspended in phosphate-buffered saline (PBS) at

47 fovir (cCDV) analogs 1-O-hexa-decyloxypropyl-CDV (HDP-CDV) and 1-O-hexadecyloxypropyl-cCDV (HDP-cCDV)

48 A V-defective CDV multiplied with reduced efficiency in lymphocytes an

49 d digestive symptoms elicited by V-defective CDV, but it was dispensable for the invasion of the lymp

50 showed that levels of cidofovir diphosphate (CDV-DP), the active antiviral compound, were >100 times

51 transition from positive to negative during CDV therapy.

52 determined for each virus by comparing each CDV-treated virus group to its respective PBS control, a

53 imal amino acids are necessary for efficient CDV F(1a/b) cleavage.

54 Using biologically equivalent CDV reduced inconsistencies to 10% (P<0.001).

55 For CDV, the proficiency of syncytium formation varies among

56 ns of respiratory disease were evaluated for CDV antigen using a direct fluorescent antibody test (FA

57 ic lesions, immunohistochemical labeling for CDV antigen, and detection of CDV RNA by reverse transcr

58 VNT-negative and -positive sera specific for CDV and PDV.

59 residues 110-114) to mediate specificity for CDV F-Lederle.

60 d to catalyze CDV diphosphate synthesis from CDV monophosphate, whereas phosphoglycerate kinase (EC 2

61 t and causes immunosuppression, we generated CDV either unable to recognize one of the receptors or i

62 HDP-CDV and HDP-cCDV were taken up rapidly by MRC-5 human lu

63 HDP-CDV seems to circumvent poor cellular uptake by rapid as

64 DV) analogs 1-O-hexa-decyloxypropyl-CDV (HDP-CDV) and 1-O-hexadecyloxypropyl-cCDV (HDP-cCDV), show >1

65 cidofovir (CDV)--hexadecyloxypropyl-CDV (HDP-CDV) and octadecyloxyethyl-CVD (ODE-CDV)--in severe comb

66 In contrast to CDV, HDP-CDV is orally bioavailable and has been reported to be o

67 rally administered treatment with either HDP-CDV or ODE-CDV is 4-8-fold more active, on a molar basis

68 DV, cCDV, and their alkoxyalkanol esters HDP-CDV and HDP-cCDV.

69 ew Zealand Red rabbits and (14)C labeled HDP-CDV.

70 were present 48 hours after the maximum HDP-CDV concentration.

71 Intravitreal pharmacokinetics of HDP-CDV in the retina, choroid, and vitreous followed a two-

72 for the increased antiviral activity of HDP-CDV in vitro, we studied the cellular uptake and anaboli

73 The safety and pharmacokinetics of HDP-CDV intravitreal injections were studied using New Zeala

74 toxicity after intravitreal injection of HDP-CDV up to 28 mug/eye.

75 e (PBS) at 37 degrees C and formation of HDP-CDV was monitored by high-performance liquid chromatogra

76 These data suggest that HDP-CDV and ODE-CDV should be further evaluated as potential

77 ysiologic conditions, slowly converts to HDP-CDV, another potent anti-CMV prodrug that may be taken u

78 When cells were exposed to HDP-CDV, the intracellular half-life of CDV-DP was 10 days v

79 % of the HDP-cyclic-CDV was converted to HDP-CDV.

80 l compound, were >100 times greater with HDP-CDV than levels observed with CDV.

81 ontribute to protection against heterologous CDV strains.IMPORTANCE Rabies virus and canine distemper

82 ogues of cidofovir (CDV)--hexadecyloxypropyl-CDV (HDP-CDV) and octadecyloxyethyl-CVD (ODE-CDV)--in se

83 DV by PCR, culture or tissue histopathology, CDV was given intravenously at 5 mg/kg weekly for 2 cons

84 us and a neighboring neutral locus, but (ii) CDV seldom indicates "selection" in neutral haplotypes w

85 ras and mutagenesis reveals four residues in CDV F-ODP (positions 164, 219, 233, and 317) required fo

86 ropensity for host-switching has resulted in CDV emergence in new species, including endangered wildl

87 ake and anabolic metabolism of (14)C-labeled CDV, cCDV, and their alkoxyalkanol esters HDP-CDV and HD

88 ent glycoproteins were protected from lethal CDV challenge, whereas all animals that received recombi

89 combinant viruses were protected from lethal CDV challenge.

90 trol eyes in the Ad5/NZ rabbit ocular model, CDV alone demonstrated a significant antiviral inhibitor

91 Negative CDV FAT results equated to 80% chances of recovery after

92 are located in two nearby clusters in a new CDV H structural model.

93 These data suggest that HDP-CDV and ODE-CDV should be further evaluated as potential antiviral a

94 CDV (HDP-CDV) and octadecyloxyethyl-CVD (ODE-CDV)--in severe combined immunodeficient mice in which e

95 istered treatment with either HDP-CDV or ODE-CDV is 4-8-fold more active, on a molar basis, than is i

96 xcellent model for evaluating the ability of CDV vaccines to protect against symptomatic infection.

97 However, the acquisition of CDV resistance did not alter the replication of the thre

98 eversed the antiviral inhibitory activity of CDV: increased mean Ad5 eye titer, increased Ad5-positiv

99 Characterization of CDV F chimeras and mutagenesis reveals four residues in

100 and animals immunized with a combination of CDV attachment protein- and fusion protein-expressing re

101 This study demonstrates the complexity of CDV dynamics in natural ecosystems and the value of long

102 ause the pathogenesis and clinical course of CDV infection of ferrets is quite similar to that of oth

103 analyzing a long-term serological dataset of CDV in lions and domestic dogs from Tanzania's Serengeti

104 d evaluation of orally active derivatives of CDV.

105 l labeling for CDV antigen, and detection of CDV RNA by reverse transcription-PCR.

106 To gain insights into the determinants of CDV pathogenesis, we isolated a strain highly virulent f

107 In all of the dogs, a diagnosis of CDV infection was established by the presence of compati

108 state-space model, we show that dynamics of CDV have changed considerably over the past three decade

109 The intracellular levels of CDV monophosphate and CDV diphosphate increased approxim

110 d to HDP-CDV, the intracellular half-life of CDV-DP was 10 days versus 2.7 days reported when cells a

111 sphate, the putative antiviral metabolite of CDV.

112 he infusions to reduce the nephrotoxicity of CDV.

113 Initially, peaks of CDV infection in dogs preceded those in lions, suggestin

114 urces, the most efficient phosphorylation of CDV monophosphate is catalyzed by pyruvate kinase.

115 was relatively unaffected by the presence of CDV, while known late capsid and tegument structural gen

116 (vIRF-1), was unaffected by the presence of CDV, while that of others, such as K4.1 (vMIP-III), K11.

117 orally active ether lipid-ester prodrugs of CDV and of (S)-HPMPA that have slight differences in the

118 a RABV expressing the attachment protein of CDV vaccine strain Onderstepoort succumbed to infection

119 y is needed to further determine the role of CDV in the treatment of ADV after HSCT.

120 ning the full-length antigenomic sequence of CDV(OS).

121 Genomic sequencing of CDV isolated from one of the infected pandas (giant pand

122 cells, presumably due to the stimulation of CDV uptake and higher activities of phosphorylating enzy

123 the neurovirulent Snyder Hill (SH) strain of CDV (CDV(SH)) and show that this virus rapidly circumven

124 in vivo spread of a neurovirulent strain of CDV provides a novel model system to study the mechanism

125 se genetics systems for wild-type strains of CDV and the use of the resulting recombinant (r) viruses

126 e distemper virus 2 and two other strains of CDV not previously detected in the continental United St

127 died the mechanism of enzymatic synthesis of CDV diphosphate, the putative antiviral metabolite of CD

128 used for preparative enzymatic synthesis of CDV monophosphate.

129 d developed the erythematous rash typical of CDV.

130 man lung fibroblasts in vitro, but uptake of CDV and cCDV was much slower.

131 etail the reliability of inferences based on CDV, using simulation and analytical methods.

132 nts of the CDV vaccine strain Onderstepoort (CDV(OS) and CDV(OL), respectively), and the MV vaccine s

133 73 was unaffected by the presence of TPA or CDV, suggesting that it was constitutively expressed.

134 Using viral outcome parameters, CDV resistance was determined for each virus by comparin

135 icated in the emergence of highly pathogenic CDV and host switching.

136 An enzymatic activity phosphorylating CDV to its monophosphate derivative was purified from hu

137 s, which concurrently display the protective CDV and RABV glycoprotein antigens.

138 on in a liver transplant recipient receiving CDV infusions.

139 To identify these cells, a recombinant CDV expressing green fluorescent protein was produced by

140 ion activity in the context of a recombinant CDV.

141 We rescued a SLAM-blind recombinant CDV with six mutations that did not infect ferret periph

142 The use of recombinant CDV(SH) (rCDV(SH)) expressing enhanced green fluorescent

143 Five of six CDV infected giant pandas died.

144 ld be an adequate screening test for suspect CDV or PDV cases and would also be useful for epidemiolo

145 ons about the clinical outcomes of suspected CDV cases, with 2-h turnaround times, by using the CDV F

146 nnecessary euthanasia of dogs with suspected CDV.

147 We present our study showing that CDV FAT results were predictive of clinical recovery (pr

148 We conclude that, although the CDV method does not appear to precisely locate selected

149 pathogenicity between the Ad5 parent and the CDV-resistant variants.

150 x of recombinant rabies viruses carrying the CDV fusion and attachment glycoproteins were protected f

151 To further characterize the CDV strains detected in the four cases, complete gene se

152 virus (ALVAC) vaccine strains expressing the CDV hemagglutinin (H) and fusion (F) protein genes (NYVA

153 total of 4,508 bases were sequenced for the CDV strains detected from each of the four cases.

154 On the other hand, the CDV IRES forms a 40S/eIF3/IRES ternary complex, with mul

155 strains and MV(Edm) were introduced into the CDV and MV genetic backgrounds, and recombinant viruses

156 Irrespective of the CDV antigens used, all animals developed protective tite

157 he Pre peptide modulates the function of the CDV F protein.

158 Application of the CDV FAT to these samples avoids unnecessary euthanasia o

159 tivated rabies viruses that carry one of the CDV glycoproteins on their surface.

160 eversal, the introduction of sections of the CDV H stalk into MV H shows a five-residue fragment (res

161 ll- and large-plaque-forming variants of the CDV vaccine strain Onderstepoort (CDV(OS) and CDV(OL), r

162 recombinant rabies viruses carrying only the CDV attachment protein according to the same immunizatio

163 Previous studies showed that the CDV and cyclic cidofovir (cCDV) analogs 1-O-hexa-decylox

164 ses, with 2-h turnaround times, by using the CDV FAT.

165 compared to 55% chances of recovery when the CDV FAT results were positive.

166 ombining the five-residue H chimera with the CDV F-ODP quadruple mutant partially restores activity,

167 Thus, antemortem examination with the CDV FAT on external epithelia of recently vaccinated, si

168 e inoculated topically in both eyes with the CDV-resistant variants R1, R2, and R3, and the Ad5 paren

169 d but useful finding was the ability of this CDV- and PDV-specific cELISA to also detect antibodies a

170 All three CDV vaccines elicited neutralizing titers of at least 1:

171 Importantly, the three CDV strains detected were demonstrated to be genetically

172 ar shedding), but had no effect on the three CDV-resistant variants.

173 Thus, CDV initially infects lymphocytes and massively replicat

174 Thus, CDV takes advantage of mucosal surfaces for host invasio

175 In contrast to CDV, HDP-CDV is orally bioavailable and has been reporte

176 2.7 days reported when cells are exposed to CDV.

177 V F highlights the MV residues homologous to CDV F residues 233 and 317 as determinants for physical

178 es; however, one strain showed similarity to CDV strains detected in a panda from China.

179 nstrate that giant pandas are susceptible to CDV and suggest that surveillance and vaccination among

180 ood viral cultures became negative after two CDV doses.

181 e differential fusogenicity of the wild-type CDV isolate 5804Han89 (CDV(5804)), the small- and large-

182 pted CDV(5804P) and the prototypic wild-type CDV(R252) showed that hematogenous infection of the chor

183 nsistent diagnoses when the approved uniform CDV was used.

184 The constrained disequilibrium values (CDV) method approaches this problem by examining differe

185 red a virus from a cDNA copy of the virulent CDV strain's consensus sequence by using a modified reve

186 y vaccinated against canine distemper virus (CDV) and develop respiratory disease.

187 Canine distemper virus (CDV) and measles virus (MV) cause severe illnesses in th

188 g antibodies against canine distemper virus (CDV) and phocine distemper virus (PDV) in sera from dogs

189 Both canine distemper virus (CDV) and rabies virus (RABV) cause lethal disease in wil

190 dai virus (SeV), and canine distemper virus (CDV) are resistant.

191 ncluding measles and canine distemper virus (CDV) are well known, but the host cells supporting infec

192 NCE Rabies virus and canine distemper virus (CDV) cause high mortality rates and death in many carniv

193 reading frame of the canine distemper virus (CDV) F protein is more complex, with a short hydrophobic

194 y based on a pair of canine distemper virus (CDV) F proteins (strains Onderstepoort (ODP) and Lederle

195 ll entry, we mutated canine distemper virus (CDV) H and identified residues necessary for efficient c

196 eport an outbreak of canine distemper virus (CDV) infection among endangered giant pandas (Ailuropoda

197 Canine distemper virus (CDV) infection of ferrets causes an acute systemic disea

198 Canine distemper virus (CDV) infection of ferrets is clinically and immunologica

199 s, disease caused by canine distemper virus (CDV) infection was suspected based on clinical signs.

200 Canine distemper virus (CDV) infects many carnivores, including ferrets and dogs

201 Canine distemper virus (CDV) is an animal morbillivirus with a worldwide circula

202 cells expressing the canine distemper virus (CDV) or mumps virus F protein.

203 ions of ferrets with canine distemper virus (CDV) recapitulate many hallmarks of measles: rash, high

204 The propensity of canine distemper virus (CDV) to spread to the central nervous system is one of t

205 lly, others, such as canine distemper virus (CDV), are a growing concern.

206 l genes (e.g., ORFs 25, 26, 64, and 67) were CDV sensitive.

207 ith cellular membrane phospholipids, whereas CDV uptake proceeds via the slow process of fluid endocy

208 fecting functionality when co-expressed with CDV H.

209 of of principle for the treatment of KS with CDV.

210 eater with HDP-CDV than levels observed with CDV.

211 gilant surveillance and early treatment with CDV can prevent the poor outcomes associated with ADV di