ライフサイエンスコーパス: CEL

1 CEL I can detect 100% of the sequence variants present,

2 CEL I, isolated from celery, is the first eukaryotic nuc

3 CEL I-like nucleases are found in many plants.

4 CEL is also a major lipase in the breast milk of many ma

5 CEL transgenic mice bred to an atherosclerosis susceptib

6 CEL-1 has a C-terminal domain containing motifs found in

7 CEL-1 shows a strong preference for RNA substrates of at

8 CEL-1 uses a mechanism similar to protein-tyrosine phosp

9 CEL-transfected cells secreted active enzyme into the me

10 We use data from >28 000 CEL files relating to 10 different Affymetrix GeneChip p

11 oxyl ester lipase (CEL) gene, resulting in a CEL protein with increased tendency to aggregate.

12 f raw microarray data files (e.g. Affymetrix CEL files) can be time consuming, complex, and requires

13 Although CEL is synthesized predominantly in the pancreas, a low

14 o the duodenum of lymph fistula CEL(+/+) and CEL(-/-) mice at a rate of 0.3 ml/h.

15 ts showed no difference between CEL(+/+) and CEL(-/-) mice in the rate of cholesterol and triglycerid

16 es of 82+/-3% and 91+/-10% (n=6) for CML and CEL respectively and, calibration curves (R(2)>0.9985) w

17 However, both control and CEL-transfected cells accumulated exogenously added CM-R

18 es the current state of knowledge of HES and CEL and the implications of the FIP1L1-PDGFRA discovery

19 Recent reports indicate that HES and CEL are imatinib-responsive malignancies, with rapid and

20 However, not all HES and CEL patients respond to imatinib, suggesting disease het

21 d lipid absorption efficiency due to PTL and CEL inactivation also resulted in protection against die

22 Thus, this study documents that PTL and CEL serve complementary functions, working together to m

23 o 61.1 +/- 3.8% in mice lacking both PTL and CEL.

24 Smart-seq, DP-seq and CEL-seq, and demonstrated significant technical variatio

25 red with commercial sample, while in TDB and CEL, phenolics remain almost unchanged.

26 s showed no difference between wild type and CEL knockout mice in the total amount of cholesterol abs

27 mulation than that observed in wild type and CEL(-/-) mice.

28 ter were not different between wild-type and CEL-deficient mice.

29 gh fat/high cholesterol diet, wild type, and CEL(-/-) mice gained approximately 24 g of body weight.

30 We found that both CEL-WT and CEL-MUT were secreted via the endoplasmic reticulum and

31 The MBR software and the sample array .CEL files used in this article are available at: http://

32 e it, suggesting that another enzyme besides CEL can hydrolyze dietary CE in mice.

33 ate that in the mouse, other enzymes besides CEL participate in the hydrolysis of dietary cholesteryl

34 een batches within an experiment and between CEL files within a batch.

35 Results showed no difference between CEL(+/+) and CEL(-/-) mice in the rate of cholesterol an

36 We found that both CEL-WT and CEL-MUT were secreted via the endoplasmic ret

37 strates indicated that those manufactured by CEL Associates, Inc. yield the highest oligo coverage.

38 induced specific DNA cleavage as measured by CEL-1 assays.

39 4 repeats in the VNTR (CEL14R) or C563fsX673 CEL (CEL maturity onset diabetes of youth with a deletio

40 eats in the VNTR (CEL14R) or C563fsX673 CEL (CEL maturity onset diabetes of youth with a deletion mut

41 er 2000 and April 2001 releases) and Celera (CEL; February 2001 release) databases.

42 that purified nucleases derived from celery (CEL I), mung bean sprouts and Aspergillus (S1) were able

43 Participants consumed less CML, CEL, and MG-H1 during the low-AGE dietary period than du

44 owed to simultaneously quantify lysine, CML, CEL and the Nepsilon-(2-Furoylmethyl)-L-lysine (furosine

45 rmal inactivation in comparison with control CEL.

46 mputational analyses, M3D provides raw data (CEL file) and normalized data downloads of each compendi

47 Data deposition: GeneChip raw data (CEL-files) have been deposited for public access in the

48 ng two of the six ORF that had been deleted: CEL ORF3 (HopPtoM) and ORF4 (ShcM).

49 We found that the loss of virulence in Delta CEL and dspA/E mutants was linked to their inability to

50 The Delta CEL mutant activated SA-dependent callose deposition in

51 nt pathogens Pseudomonas syringae (the Delta CEL mutation), Erwinia amylovora (the dspA/E mutation),

52 e also compare with Smart-Seq to demonstrate CEL-Seq2's increased sensitivity relative to other avail

53 refore investigated nondiabetic and diabetic CEL-mutation carriers by pancreatic imaging studies and

54 Mice with different CEL genotypes [wild type (WT), knockout (CELKO), heteroz

55 ncreatic enzyme activities related to either CEL or PTL were separated using DEAE-chromatography.

56 The Caenorhabditis elegans CEL-1 capping enzyme consists of an N-terminal region wi

57 on due to mutations in the CEL gene encoding CEL.

58 operty of the mismatch-specific endonuclease CEL I to cleave heteroduplex DNA with a very high specif

59 gene with the mismatch specific endonuclease CEL I.

60 cked at mismatched sites by the endonuclease CEL I and cut strands are visualized using Li-cor gel an

61 were transfected with constructs expressing CEL with 14 repeats in the VNTR (CEL14R) or C563fsX673 C

62 terials for converting the raw data files (*.CEL) for comparative analysis.

63 s infused into the duodenum of lymph fistula CEL(+/+) and CEL(-/-) mice at a rate of 0.3 ml/h.

64 assessed the highest antioxidant effect for CEL with starters (21.7mg Trolox eq/g FW; 8.5mumol hydro

65 vel and physiologically significant role for CEL, namely the promotion of large chylomicron productio

66 Other suggested roles for CEL include the direct facilitation of the intestinal ab

67 total CML were three times higher than free CEL levels.

68 PMaP (SNP microarrays and pooling) data from CEL files to Relative Allele Scores in the rich R statis

69 the presence of bile salt and distinct from CEL, is present, compatible with the results from our ac

70 Peritoneal macrophages from CEL transgenic mice displayed a 4-fold increase in [(3)H

71 Here we characterize a gene (CEL-1) believed to encode the C. elegans capping enzyme.

72 t that F/P is not sufficient to induce a HES/CEL-like disease but requires a second event associated

73 gel electrophoresis band for the homogeneous CEL I, with and without the removal of its carbohydrate

74 r of wild-type, heterozygous, and homozygous CEL-deficient mice, the levels of bile salt-dependent re

75 However, CEL(-/-) mice produced predominantly smaller lipoprotein

76 However, CEL-1 has no detectable PTP activity.

77 region that resembles known GTases, However, CEL-1 has not previously been shown to have GTase activi

78 hydrolytic activity inhibited by anti-human CEL IgG.

79 We implemented CEL-Seq2 on Fluidigm's C1 system, providing its first si

80 could find structural pancreatic changes in CEL-MODY subjects during the course of diabetes developm

81 nd accumulation of neoplastic eosinophils in CEL.

82 s of ceramide and lysophosphatidylcholine in CEL-expressing cells than in CEL-negative cells.

83 ide hydrolytic activity than that present in CEL(+/+) mice.

84 C and cyanidine-3-glucoside and quercetin in CEL.

85 We also studied tissue uptake of CM-RE in CEL-deficient mice generated by targeted disruption of t

86 show the involvement of arginine residues in CEL-bile salt interaction.

87 tidylcholine in CEL-expressing cells than in CEL-negative cells.

88 ' linkages in SRELs were higher than that in CEL.

89 sing and absorption of RE were unimpaired in CEL-deficient mice.

90 Full-length CEL-1 has RTPase and GTase activities, and the cDNA can

91 ome (HES) and chronic eosinophilic leukemia (CEL) comprise a spectrum of indolent to aggressive disea

92 F/P-positive chronic eosinophilic leukemia (CEL) in humans.

93 Chronic eosinophilic leukemia (CEL) is a myeloproliferative neoplasm characterized by e

94 GFRA-positive chronic eosinophilic leukemia (CEL), little is known about optimal dosing, duration of

95 osis (SM) and chronic eosinophilic leukemia (CEL)/hypereosinophilic syndrome (HES).

96 A cellulolytic enzyme lignin (CEL) was also prepared as a comparison.

97 SP, like CEL I, does not turn over after incision at a mismatched

98 le salt activation of carboxyl ester lipase (CEL) activity.

99 the contributions of carboxyl ester lipase (CEL) and pancreatic triglyceride lipase (PTL) in lipid n

100 the last exon of the carboxyl ester lipase (CEL) gene, resulting in a CEL protein with increased ten

101 Pancreatic carboxyl ester lipase (CEL) hydrolyzes cholesteryl esters (CE), triglycerides (

102 To study the role of carboxyl ester lipase (CEL) in hepatic retinoid (vitamin A) metabolism, we inve

103 Carboxyl-ester lipase (CEL) maturity-onset diabetes of the young (MODY) is a mo

104 peat (VNTR) domain of carboxyl ester lipase (CEL) presents an opportunity to study the pathogenesis o

105 patocytes showed that carboxyl ester lipase (CEL), a broad-spectrum lipase secreted by pancreas and l

106 tion of R-9-PAHSA and carboxyl ester lipase (CEL), a PAHSA degradative enzyme, selectively hydrolyzin

107 Bile salt-stimulated carboxyl ester lipase (CEL), also called cholesterol esterase, is one of the ma

108 iously we showed that carboxyl ester lipase (CEL)-deficient (CELKO) mice have normal levels of pancre

109 Carboxyl ester lipase (CEL, also called cholesterol esterase or bile salt-depen

110 Carboxyl ester lipase (CEL; EC 3.1.1.13) hydrolyzes cholesteryl esters and reti

111 n that mutations in conserved effector loci (CEL) in the plant pathogens Pseudomonas syringae (the De

112 n of one ipx gene, conserved effector locus (CEL) orf1, encoding a putative lytic murein transglycosy

113 ORF) in the DC3000 conserved effector locus (CEL) reduces parasitic growth and abolishes disease symp

114 locus (EEL) and a conserved effector locus (CEL).

115 l)lysine (CML), N(euro)-(carboxyethyl)lysin (CEL), and methylglyoxal-derived hydroimadazolidine (MG-H

116 ne (CML), N(epsilon)-(1-carboxyethyl)lysine (CEL) and N(delta)-(5-hydro-5-methyl-4-imidazolon-2-yl)-o

117 ine (CML), Nepsilon-(Carboxyethyl)-L-lysine (CEL).

118 and free Nepsilon-(1-Carboxyethyl)-L-Lysine (CEL).

119 and physiological significance of macrophage CEL expression.

120 s study used transgenic mice with macrophage CEL expression at levels comparable with that observed i

121 Collectively, the improvements make CEL-Seq2 uniquely suited to single-cell RNA-Seq analysis

122 K), MODY3 (HNF1A), MODY5 (HNF1B), and MODY8 (CEL) with a polycistronic lentiviral vector expressing a

123 SM development and pathogenesis in a murine CEL model induced by F/P in hematopoietic stem cells and

124 ution of the wild type (WT) and mutant (MUT) CEL proteins in cellular models.

125 studies revealed less R63A and R423G mutant CEL were bound to 1,2-diolein monolayer at saturation co

126 system, and after its secretion, the mutant CEL protein was re-internalized, transported to the lyso

127 rmite di Bitetto (TDB) and Cellina di Nardo (CEL) were studied, highlighting also the cultivars influ

128 sent study, we further investigated this non-CEL REH activity in pancreas homogenates of CELKO and wi

129 ent for mutation detection, a novel nuclease CEL I from celery.

130 ignificantly increased RCT in the absence of CEL and suggest a novel mechanism by which to manipulate

131 In contrast, the addition of CEL to the apical medium increased the amount of large l

132 Colocalization of CEL in the vicinity of OxLDL formation was supported by

133 We measured the concentration of CEL in human plasma as 1.2+/-0.5 ng/ml (in the range rep

134 The N-terminal domain of CEL-1 has RNA triphosphatase activity.

135 The effect of CEL on RCT of HDL cholesterol was assessed by measuring

136 Expression of CEL MODY increased endoplasmic reticulum stress, activat

137 te] were generated to study the functions of CEL in a physiological system.

138 Homologies of CEL I with S1 and P1 nucleases are much lower.

139 Internalization of CEL-MUT also led to reduced viability of pancreatic acin

140 The proximal intestine of CEL(-/-) mice was also found to possess significantly le

141 These data indicate that the lack of CEL expression does not affect the uptake of dietary CM-

142 nce in C. elegans embryos shows that lack of CEL-1 causes development to arrest with a phenotype simi

143 redominantly in the pancreas, a low level of CEL expression was reported in human macrophages.

144 ticipate in micellar bile salt modulation of CEL enzymatic activity through intramolecular hydrogen b

145 /P-dependent proteins in the pathogenesis of CEL.

146 The physiological role of CEL was originally thought to be its mediation of dietar

147 Secretion of CEL MODY was decreased compared with that of CEL14R.

148 However, the x-ray crystal structure of CEL failed to show the involvement of arginine residues

149 subcellular distributions differed, as only CEL-MUT was observed as an aggregate at the cell surface

150 present CEL-Seq2, a modified version of our CEL-Seq method, with threefold higher sensitivity, lower

151 In vivo, pancreatic CEL is thought to liberate cholesterol and retinol from

152 lipids were incubated with purified porcine CEL without or with cholate (10 or 100 microM, concentra

153 T-cell overexpression of IL-5 (F/P-positive CEL mice).

154 clonal population in FIP1L1/PDGFRA-positive CEL and suggest that molecular monitoring may be the mos

155 ions, 5 patients with FIP1L1/PDGFRA-positive CEL with documented clinical, hematologic, and molecular

156 Here, we present CEL-Seq2, a modified version of our CEL-Seq method, with

157 0% in PTL(-/-) mice (p < 0.05) and PTL(-/-), CEL(-/-) mice (p < 0.01), respectively.

158 e body weight gain in PTL(-/-) and PTL(-/-), CEL(-/-) mice was attributed to their absorption of fewe

159 g less (p < 0.01) in PTL(-/-) and PTL(-/-), CEL(-/-) mice, respectively, despite their consumption o

160 difference was not exaggerated in PTL(-/-), CEL(-/-) mice.

161 We have purified CEL I 33 000-fold to apparent homogeneity.

162 -RH7777) cells stably transfected with a rat CEL cDNA.

163 alt-independent hydrolytic activities of rat CEL.

164 Since CEL is expressed mainly in pancreatic acinar cells, we a

165 paediatric AIDs (pAIDs), including JIA, SLE, CEL, T1D, UC, CD, PS, SPA and CVID, attributable to comm

166 the understanding of how the acinar-specific CEL-MUT protein causes both exocrine and endocrine pancr

167 Meanwhile, a mismatch-specific CEL II enzyme (Surveyor((R)) nuclease) cleaved the imper

168 inophilic leukemia, not otherwise specified (CEL, NOS) is assigned to patients with MPN with eosinoph

169 be confined to the gastrointestinal system, CEL has been reported in the plasma of humans and other

170 We conclude that CEL has the capability to modify normal human LDL and HD

171 These studies documented that CEL expression in macrophages is pro-atherogenic and tha

172 The current study tests the hypothesis that CEL in the intestinal lumen may influence the type of li

173 , was required for absorption, implying that CEL was not the responsible enzyme.

174 Our results indicate that CEL I mutation detection is a highly sensitive method fo

175 ious tissues did not differ, indicating that CEL deficiency does not affect hepatic retinoid metaboli

176 and 1.6+/-0.1, respectively, indicating that CEL interaction varied with lipoprotein class.

177 We propose that CEL I exemplifies a new family of neutral pH optimum, ma

178 The CEL carries at least seven ORFs that are conserved betwe

179 The CEL-1 RTPase domain is related by sequence to protein-ty

180 28% of the CEL I polypeptide, we cloned the CEL I cDNA.

181 .5-fold higher in macrophages expressing the CEL transgene.

182 of A-T-rich regions distinguish SP from the CEL I family of neutral pH mismatch endonucleases recent

183 re found for 5% and 2% of the markers in the CEL and HGP-sc sequences, respectively.

184 exocrine dysfunction due to mutations in the CEL gene encoding CEL.

185 The hopPtoM and avrE genes in the CEL of P. syringae were found to encode suppressors of t

186 nic lysophosphatidylcholine was lower in the CEL transgenic mice, but plasma cholesterol level and li

187 and frameshift mutation (C563fsX673) in the CEL VNTR causes CP through proteotoxic gain-of-function

188 mice generated by targeted disruption of the CEL gene.

189 w and developed normally, independent of the CEL genotype of the pup or nursing mother.

190 SP nuclease may be a natural variant of the CEL I family of mismatch endonucleases is discussed.

191 ion of the amino acid sequence of 28% of the CEL I polypeptide, we cloned the CEL I cDNA.

192 results, along with computer modeling of the CEL protein, indicated that Arg(63) and Arg(423) are not

193 , whereas deletion of a large portion of the CEL strongly reduces growth and abolishes pathogenicity

194 dominantly smaller lipoproteins, whereas the CEL(+/+) mice produced primarily large chylomicrons and

195 lesion area than apoE(-/-) mice without the CEL transgene when both were fed a high fat/cholesterol

196 nd removal of various blob defects from the .CEL files of different types of Affymetrix microarrays.

197 id content were similar in mice of all three CEL genotypes.

198 The mechanism appears to be mediated through CEL hydrolysis of ceramide generated during the lipid ab

199 inly to PTL, and to a much smaller extent to CEL.

200 Similar to CEL I, the presence of a DNA polymerase or a DNA ligase

201 aled to form heteroduplexes and subjected to CEL I incision.

202 ases in k(cat), in comparison with wild type CEL, for bile salt-dependent cholesteryl ester hydrolysi

203 es, respectively, in comparison to wild type CEL.

204 olayer at saturation compared with wild type CEL.

205 In both cell types, CEL MODY formed intracellular aggregates.

206 le method of enzyme mutation detection using CEL I can efficiently identify mutations and polymorphis

207 into cholesteryl [(3)H]oleate compared with CEL-negative macrophages when the cells were incubated u

208 nd only in rats and WT mice, consistent with CEL-mediated cholesteryl ester hydrolysis.

209 After a 4-h incubation with CEL, the lysoPC content of OxLDL was depleted 57%.

210 These findings show that subjects with CEL-MODY develop multiple pancreatic cysts by the time t