ライフサイエンスコーパス: COPI

1 COPI and clathrin represent two important and distinct s

2 COPI coated vesicles carry material between Golgi compar

3 COPI coated vesicles mediate trafficking within the Golg

4 COPI in these mutants is released from Golgi membranes b

5 COPI is a coatomer protein complex responsible for intra

6 COPI is required for ER-Golgi transport and early endoso

7 COPI mediates retrograde trafficking from the Golgi to t

8 COPI proteins oligomerize to form coated vesicles that t

9 COPI subunits immunoprecipitated with KA2 subunits from

10 COPI vesicles are generated through activation of the re

11 COPI-coated vesicles form at the Golgi apparatus from tw

12 COPI-coated vesicles mediate trafficking within the Golg

13 des a subunit of coatomer protein complex 1 (COPI) involved in intracellular traffic and autophagy.

14 ve used both site-directed mutagenesis and a COPI complex precipitation assay to demonstrate that int

15 ly, followed approximately 10 min later by a COPI component (epsilon-COP) and a trans-Golgi network (

16 Finally, a COPI binding domain swap was used to demonstrate that su

17 termediates proceeds in two stages: first, a COPI-independent event leads to the formation of an unst

18 ulum and mitochondrial inheritance and for a COPI coatomer subunit in the targeting of a type V myosi

19 we find that mTRAPPII binds to gamma1COP, a COPI coat adaptor subunit.

20 the glycoprotein ERAD signal is generated, a COPI-binding motif was appended to the N terminus of the

21 is a secondary consequence of a defect in a COPI-dependent retrograde pathway.

22 , implying that Trs120p may participate in a COPI-dependent trafficking step on the early endosomal p

23 suggest that cisternal maturation involves a COPI-dependent pathway that recycles early Golgi protein

24 Assembly of a COPI coated vesicle is initiated by the small GTPase Arf

25 l requires either COPI coatomer complex or a COPI subcomplex for translocation from the endosomal lum

26 Adv-COPI protected distal and proximal tubules against hypox

27 Adv-COPI significantly improved renal function by restoring

28 Adv-COPI treatment selectively augmented COX-1 and PGIS prot

29 After Adv-COPI transfection, we evaluated the renal COX-1 and PGIS

30 t PGI2 production by intrarenal arterial Adv-COPI administration with renal venous clamping in female

31 Increased PGI2 by Adv-COPI protects the kidney against I/R-induced oxidative s

32 se (COX)-1/prostacyclin synthase (PGIS) (Adv-COPI) gene transfer in rat kidneys with ischemia-reperfu

33 ciation of GBF1 with this compartment allows COPI recruitment and leads to its maturation into transp

34 ng clathrin-mediated entry is caveolin-1 and COPI dependent.

35 f TSET, a 'missing link' between the APs and COPI.

36 oid legs radiating from a common center, and COPI shares with COPII highly similar vertex interaction

37 related to those of clathrin heavy chain and COPI subunits.

38 COG and COPI may work in concert to ensure the proper retention

39 ticulum (ER) export of proteins in COPII and COPI vesicles, respectively.

40 of coat protein complex II and I (COPII and COPI).

41 CUPS biogenesis is independent of COPII- and COPI-mediated membrane transport.

42 endent trafficking through the endocytic and COPI systems.

43 V5-tagged TMEM199 localized with ERGIC and COPI markers in HeLa cells, and electron microscopy of a

44 G complex physically interacts with GS28 and COPI and specifically binds to isolated CCD vesicles.

45 tors markedly reduced association of KA2 and COPI.

46 of Golgi membranes with mitotic kinases and COPI coat proteins efficiently disassembles the membrane

47 tether that connects cis-Golgi membranes and COPI-coated, retrogradely targeted intra-Golgi vesicles.

48 mediated by the interaction of RXR motif and COPI.

49 In Arabidopsis, COPI and COPII vesicle coat proteins as well as vesicle

50 A subset of the Arf1-COPI vesicular transport proteins also regulated droplet

51 Our findings uncover a function for Arf1/COPI proteins at LDs and suggest a model in which Arf1/C

52 Cells lacking Arf1/COPI function have increased amounts of phospholipids on

53 Recent evidence shows that Arf1/COPI can bud nano-LDs ( approximately 60 nm diameter) fr

54 We show that Arf1/COPI proteins localize to cellular LDs, are sufficient t

55 The Arf1/COPI protein machinery, known for its role in vesicle tr

56 ins at LDs and suggest a model in which Arf1/COPI machinery acts to control ER-LD connections for loc

57 stituted vesicles is at least as abundant as COPI and that GAP binds directly to the dilysine motif o

58 Identification as COPI vesicles was based on colocalization with beta-COP,

59 allows peripheral membrane proteins such as COPI to be sequestered rapidly by adding rapamycin.

60 se that neurons utilize the Golgi-associated COPI vesicle to deliver cargoes necessary for motor neur

61 Association between COPI proteins and KA2 subunits was significantly reduced

62 omponents are structurally conserved between COPI and clathrin/adaptor proteins.

63 esting a possible direct interaction between COPI and lipid droplets.

64 he molecular nature of the interface between COPI and the nuclear pore has not been fully elucidated.

65 efinitively explore the relationship between COPI and CFTR in epithelial cells, we depleted beta-COP

66 ransport is achieved, and that bidirectional COPI transport is modulated by environmental cues throug

67 nals, and indeed the dimerized peptide binds COPI in vitro.

68 Blocking COPI recruitment to membranes by expressing an inactive

69 transmembrane helix 1 are essential for both COPI complex binding and the delivery of the catalytic d

70 by the addition of partially purified bovine COPI to the translocation assay mixture.

71 al in cells expressing the N121I mutant, but COPI is compromised, as shown by the release of beta-COP

72 e transport to endoplasmic reticulum (ER) by COPI-coated vesicles.

73 ifferent from transport vesicle formation by COPI, likely responsible for the diverse lipid droplet p

74 light on the structure of vesicles formed by COPI protein complexes.

75 ediate in design between COPII and clathrin: COPI shares with clathrin an arrangement of three curved

76 ponents, including the Class I vesicle coat (COPI), the spliceosome, the proteasome, the nuclear pore

77 e dynamic processes such as coatomer-coated (COPI) vesicle-mediated trafficking.

78 nt interaction between DRD3 and the coatomer COPI, a complex involved in membrane transport, and shif

79 the classical Golgi coat proteins coatomer (COPI) and clathrin.

80 By analogy to the coatomer (COPI)-independent transport of Golgi enzymes to the endo

81 , as well as with Golgi vesicle coat complex COPI.

82 into the ER, and the coat protein complexes (COPI and COPII), which mediate vesicular transport of pr

83 PII vesicles, whereas the periphery contains COPI vesicles.

84 as trafficking that circumvents conventional COPI-, COPII-, and microtubule-dependent vesicular trans

85 ae with IAP (inhibitor of apoptosis) or COP (COPI coatomer, beta subunit) dsRNA silenced their target

86 ine the RNA-binding profile of Golgi-derived COPI in neuronal cells, we performed formaldehyde-linked

87 nts by heterotypic fusion with Golgi-derived COPI vesicles.

88 plex-dependent fusion of retrograde-directed COPI vesicles.

89 , Nup153, plays a critical role in directing COPI to the nuclear membrane at mitosis and that this ev

90 retrograde protein trafficking, we disrupted COPI functions in the Yellow Fever mosquito Aedes aegypt

91 Here we found that disrupting COPI function by RNAi inhibited an early stage of vesicu

92 , co-occurring KRAS and LKB1 mutation-driven COPI addiction, and selective sensitivity to a synthetic

93 elivery of LF to the cytosol requires either COPI coatomer complex or a COPI subcomplex for transloca

94 The heterotetrameric AP and F-COPI complexes help to define the cellular map of modern

95 at the mu-homology domain is dispensable for COPI function in the early secretory pathway, whereas th

96 ation of cargo binding have implications for COPI coat assembly.

97 ual zinc finger is the minimal interface for COPI association, although tandem zinc fingers are optim

98 These data suggest a model for COPI-independent intra-Golgi transport by cisternal matu

99 gs not only identify a new factor needed for COPI vesicle formation from Golgi membrane but also reve

100 ARF1 is a small GTPase that is required for COPI vesicle formation from the Golgi membranes.

101 These include a noncanonical role for COPI, a previously uncharacterized protein complex affec

102 ivity of Arf, they govern vesicle formation, COPI trafficking and the maintenance of the Golgi comple

103 Whereas coatomer forms COPI vesicles in the host early secretory system, vaccin

104 ed 3D structure of a synthetically generated COPI vesicle coat obtained using cryoelectron tomography

105 ides its well-established role in generating COPI vesicles, we find that ARF1 is also involved in the

106 Furthermore, TC10 directly bound to Golgi COPI coat proteins through a dilysine motif in the carbo

107 However, COPI function enhanced VTC assembly, and early VTCs acqu

108 daptor complexes and coat protein complex I (COPI) and COPII self-assemble to deform the membrane and

109 factor (Arf) and the coat protein complex I (COPI) are involved in vesicle transport.

110 les appeared to be vesicular coat complex I (COPI) coated.

111 ce resembles classic coat protein complex I (COPI) coatomer protein-binding KKXX signals, and indeed

112 effects, such as the coat protein complex I (COPI) complex, the ribosome and the proteasome.

113 The coat protein complex I (COPI) has been implicated in the anterograde and retrogr

114 dibasic motif bound the coatomer complex I (COPI) in an in vitro binding assay, suggesting that ER r

115 Unexpectedly, coat protein complex I (COPI) is required for lipid droplet targeting of some pr

116 ligand-sensitive coatomer protein complex I (COPI) retrograde trafficking complex in vitro Extensive

117 RNA helicase DDX24, and coatomer complex I (COPI) subunit ARCN1 most significantly inhibited growth

118 the gamma subunit of coat protein complex I (COPI) that is responsible for Golgi-to-ER retrograde car

119 oteins including coatomer protein complex I (COPI) to the reaction mixture.

120 we identify the coatomer protein complex I (COPI) vesicle coat as a critical mechanism for retention

121 rt through tethering coat protein complex I (COPI) vesicles.

122 llular transport via coat protein complex I (COPI), we show that COPA variants impair binding to prot

123 hat knockdown of the coat protein complex I (COPI)-Arf79F (also known as Arf1) complex selectively ki

124 Coat protein complex I (COPI)-coated vesicles, one of three major types of vesic

125 We have shown that coat protein complex I (COPI)-dependent trafficking, an early step in Golgi-to-e

126 e relevance to AD of coat protein complex I (COPI)-dependent trafficking, an early step in Golgi-to-e

127 Traffic of Kv4.2 was coat protein complex I (COPI)-dependent, but KChIP1-containing vesicles were not

128 e-directed cargo but contain coat protein I (COPI) and the recycling protein p53/p58, suggesting that

129 for vesicle formation by the coat protein I (COPI) complex - a finding that reveals an unanticipated

130 Here we show that the coat protein I (COPI) complex sorts anterograde cargoes into these tubul

131 at least two subunits of the coat protein I (COPI) complex.

132 efect in the localization of coat protein I (COPI) subunits, implying that Trs120p may participate in

133 In contrast, cytosolic coat protein I (COPI) vesicle coat mutations in sey1Delta cells caused n

134 to the alpha-subunit of the coat protein I (COPI) vesicle coat protein.

135 and COPb proteins of the coatomer protein I (COPI) vesicle complex were reported to interact with spe

136 recent data showing that coatomer protein I (COPI) vesicle transport is involved in cellular processe

137 Coat protein I (COPI) vesicles arise from Golgi cisternae and mediate th

138 RF1) during the formation of coat protein I (COPI) vesicles has been unclear.

139 decreased interaction of coatomer protein I (COPI) with the hKOPR and abolished 14-3-3zeta-mediated r

140 The core complex of Coat Protein I (COPI), known as coatomer, is sufficient to induce coated

141 for the interaction with coatomer protein I (COPI), which was inversely correlated with the 14-3-3 bi

142 of coatomer and formation of coat protein I (COPI)-coated vesicles is crucial to homeostasis in the e

143 omposition of Golgi-derived coat protomer I (COPI)-coated vesicles after activating or inhibiting sig

144 d vesicles, while the COat Protein I and II (COPI and COPII) routes stand for the bidirectional traff

145 t cycles between the ER and Golgi complex in COPI and COPII vesicles, is mislocalized to the vacuole

146 ear pore basket, was found to be involved in COPI recruitment, but the molecular nature of the interf

147 ation of 12 SNPs and 24 mutations located in COPI genes linked to an increased AD risk.

148 ations of SEC36 are lethal with mutations in COPI subunits, indicating a functional connection betwee

149 Finally, the reduction in COPI binding was correlated with an increased associatio

150 are known to be involved in cargo sorting in COPI transport.

151 uctures of membrane protein coats, including COPI, have been extensively studied with in vitro recons

152 ota/lambda and soluble components, including COPI (coatomer and ADP-ribosylation factor), results in

153 intracellular trafficking systems, including COPI, COPII, and clathrin complexes.

154 sults suggest that Rab1b function influences COPI recruitment.

155 omotes expression of the hKOPR by inhibiting COPI and RVR motif-mediated endoplasmic reticulum locali

156 Similarly, inhibiting COPI dissociation from membranes by expressing a constit

157 ase 1 and alpha-2,6-sialyltransferase 1 into COPI vesicles.

158 t with GOLPH3, was neither incorporated into COPI vesicles nor was dependent on GOLPH3 for proper loc

159 e mutant, converted the Golgi membranes into COPI vesicles.

160 COP and beta'-COP subunits and packaged into COPI-coated vesicles for Golgi-to-ER retrieval.

161 ion as recycling signal to sort a SNARE into COPI vesicles in a non-degradative pathway.

162 One well-studied example is COPI or coatomer, a heptameric protein complex that is r

163 cipated mechanistic flexibility in mammalian COPI transport.

164 el role for Rab1b in ARF1- and GBF1-mediated COPI recruitment pathway.

165 s early Golgi proteins, followed by multiple COPI-independent pathways that recycle late Golgi protei

166 We find that a substantial amount of COPI is associated with Golgi membranes in the gea2-ts m

167 Pase, which is known to regulate assembly of COPI coat complexes on Golgi cisternae.

168 The assembly of COPI into a cage-like lattice sculpts membrane vesicles

169 4-3-3zeta knockdown increased association of COPI with the hKOPR.

170 Budding of COPI-coated vesicles from Golgi membranes requires an Ar

171 Temperature-sensitive degradation of COPI complex proteins was correlated with an increase in

172 which encodes the coatomer subunit delta of COPI.

173 ccumulates in the ERGIC in cells depleted of COPI.

174 plet phenotypes associated with depletion of COPI subunits.

175 rdinates sequential tethering and docking of COPI vesicles by first using long tethers (Golgins) and

176 The conformational dynamics of COPI during cargo capture and vesicle formation is incom

177 We have reconstituted the formation of COPI vesicles by incubating Golgi membrane with purified

178 mains that are connected to the formation of COPI-containing transport intermediates.

179 These data support the novel function of COPI in inter-compartmental trafficking of RNA.

180 transport by targeting the dual functions of COPI in cargo sorting and carrier formation.

181 Immunodepletion of COPI coatomer complex and associated proteins from cytos

182 Our findings demonstrate the importance of COPI-mediated transport in human development, including

183 Acute inhibition of COPI complex recruitment to the Golgi apparatus with pha

184 ermediates was not impaired by inhibition of COPI vesicle formation.

185 g brefeldin A (BFA), a chemical inhibitor of COPI function, we demonstrate that short-term (1-h) BFA

186 of proteins that influence the lifecycle of COPI-coated vesicles; this conclusion is supported by th

187 Loss of COPI causes defects in early endosome function, as both

188 Although loss of COPI results in the fragmentation of the Golgi, this doe

189 bition of early endosome function by loss of COPI subunits (beta', beta, or alpha) results in accumul

190 By simultaneous masking of COPI and endocytic signals, we were able to generate a s

191 To explore the mechanism of COPI action in CFTR traffic we tested whether CFTR was C

192 be reversed by expressing known mediators of COPI recruitment, the GTPase ARF1 and its guanine nucleo

193 imary sequence analysis, supports a model of COPI function with significant structural and mechanisti

194 ylation factor (ARF) mediated recruitment of COPI to membranes plays a central role in transport betw

195 ARF activation to facilitate recruitment of COPI to membranes, whereas GBF1 localized at the TGN med

196 f GEFs, is responsible for the regulation of COPI-mediated events at the ER-Golgi interface.

197 er, these demonstrate the functional role of COPI association with the SMN protein in neuronal develo

198 Here, we investigated the role of COPI in CFTR trafficking.

199 between Golgi compartments, but the role of COPI in the secretory pathway has been ambiguous.

200 To study the role of COPI transport in ovarian development, we injected gamma

201 grade and retrograde cargoes are the size of COPI vesicles, contain coatomer, and functionally requir

202 plays a critical role in the fission step of COPI vesicle formation from Golgi membrane.

203 Goldberg present X-ray crystal structures of COPI suggesting that these coats combine selected featur

204 SEC28 encodes the epsilon-COP subunit of COPI (coat protein complex I) coatomer proteins.

205 s similar to a region in the beta-subunit of COPI coatomer complexes, which suggests the existence of

206 hat the alpha-, beta-, and gamma-subunits of COPI co-immunoprecipitated with CFTR.

207 ts and alpha, beta', and epsilon subunits of COPI, and trace the origins of the IFT-A, IFT-B, and the

208 rotein shell that encompasses the surface of COPI vesicles.

209 on in midgut, fat body, and ovary tissues of COPI-deficient mosquitoes.

210 d is essential for the retrograde traffic of COPI-coated vesicles from the Golgi to the ER.

211 produced evidence for two distinct types of COPI vesicles, but the in vivo sites of operation of the

212 al approach, we can distinguish two types of COPI vesicles, COPIa and COPIb.

213 mTRAPPII is enriched on COPI (Coat Protein I)-coated vesicles and buds, but not

214 a suggest that the heterodimer is exposed on COPI vesicles, while the remaining part of the B-subcomp

215 domain of p115 links the Golgins, Giantin on COPI vesicles, to GM130 on Golgi membranes.

216 ctable Golgi membranes, preexisting VTCs, or COPI function.

217 ined using dsRNA directed against five other COPI coatomer subunits (alpha, beta, beta', delta, and z

218 rectly imparts membrane curvature to promote COPI tubule formation.

219 ructural model of the in vitro reconstituted COPI coat (Dodonova et al., 2017.

220 hat is independent of its role in recruiting COPI for retrograde transport, at least of a subset of G

221 GBF1 recruits COPI to pre-Golgi and Golgi compartments, whereas BIG1 a

222 hat selectively abrogate SMN binding, retain COPI-mediated Golgi-ER trafficking functionality, but we

223 ors, revealing the existence of two separate COPI-dependent pathways.

224 Among these proteins, several COPI coatomer subunits (alpha, beta, gamma, and delta) a

225 chemical studies suggested that the tapa-sin-COPI interaction regulates the retrograde transport of i

226 oci in several protein complexes (e.g., SPT, COPI, and ribosome) was posttranscriptionally controlled

227 proteins of the golgin family help to tether COPI vesicles to Golgi membranes.

228 ose that mTRAPPII is a Rab1 GEF that tethers COPI-coated vesicles to early Golgi membranes.

229 tants yielded the surprising conclusion that COPI was dispensable both for the secretion of certain p

230 Our results demonstrate that COPI function in sorting of at least three retrograde ca

231 Here, we find that COPI coat components can bud 60-nm triacylglycerol nanod

232 We found that COPI defects disrupt epithelial cell membrane integrity,

233 ion of the vesicular transport model is that COPI vesicles are responsible for trafficking anterograd

234 Our findings further reveal that COPI tubular transport complements cisternal maturation

235 Video fluorescence microscopy revealed that COPI inactivation causes an early Golgi protein to remai

236 Here we show that COPI binds K63-linked polyubiquitin and this interaction

237 Together, these data show that COPI functions are critical to mosquito blood digestion

238 led to biochemical experiments, we show that COPI subunit delta (delta-COP) affects the biology of AP

239 Taken together, our findings suggest that COPI complexes likely function indirectly in influenza v

240 The data suggest that COPI vesicles traffic both small secretory cargo and ste

241 The COPI coat forms transport vesicles from the Golgi comple

242 The COPI coatomer complex, which plays a major role in membr

243 The COPI, COPII, and clathrin cargo adaptors are structurall

244 er protein disulfide isomerase (PDI) and the COPI coat protein beta-COP.

245 on between the Sec34p/sec35p complex and the COPI vesicle coat.

246 Vesiculation is mediated by the COPI budding machinery ARF1 and the coatomer complex.

247 quire biosynthetic membrane transport by the COPI coatomer complex for efficient replication.

248 he Golgi apparatus is likely mediated by the COPI vesicle coat complex, but the mechanism is poorly u

249 and ubiquitously expressed gene encoding the COPI-associated protein pseudokinase SCYL1, causes an ea

250 g Xenopus extracts, we report a role for the COPI coatomer complex in nuclear envelope breakdown, imp

251 alpha-COP binds to SMN, linking the COPI vesicular transport pathway to SMA.

252 hat the trimer constitutes the vertex of the COPI cage.

253 veraging, we determined the structure of the COPI coat assembled on membranes in vitro at 9 A resolut

254 yeast orthologue of the gamma subunit of the COPI coat complex (Sec21p), a known Arf1p effector.

255 les in the assembly-disassembly cycle of the COPI coat in vivo.

256 ing to determine the native structure of the COPI coat within vitrified Chlamydomonas reinhardtii cel

257 rmation by functioning as a component of the COPI coat.

258 P, which is best known as a component of the COPI coatamer complexes that are required for the retrog

259 s with at least zeta-COP and beta-COP of the COPI coatomer complex.

260 arboxyl terminus of the gamma subunit of the COPI complex (gammaCOP) and describe the X-ray crystal s

261 In contrast, knockdown of the COPI complex does not hinder craniofacial morphogenesis.

262 Modeling of the COPI subunit betaCOP based on the clathrin adaptor AP2 s

263 it delta-COP function, the moderation of the COPI-dependent trafficking in vivo leads to a significan

264 Secretory protein trafficking relies on the COPI coat, which by assembling into a lattice on Golgi m

265 influenza virus infection cycle rely on the COPI complex.

266 In both organisms, the COPI-type vesicles were further characterized by a combi

267 Golgi-to-ER recycling of WLS requires the COPI regulator ARF as well as ERGIC2, an ER-Golgi interm

268 Our model proposes that the COPI cage is intermediate in design between COPII and cl

269 Our results indicate that the COPI complex plays a critical role in CFTR trafficking t

270 al and molecular experiments showed that the COPI retrograde complex regulates ligand-mediated AR tra

271 ng at the Golgi involves p115 binding to the COPI coat.

272 e show that WDR35 has strong homology to the COPI coatamers involved in vesicular trafficking and tha

273 e lysine-rich transmembrane helix 1 with the COPI binding portion of the p23 adaptor cytoplasmic tail

274 does not influence its interaction with the COPI coat or efficient recruitment onto transport vesicl

275 plasmic reticulum (ER) in a complex with the COPI protein subunit beta-COP1.

276 the same entry defects as observed with the COPI-depleted cells but did result in specific decreases

277 Golgi membranes that are liberated of their COPI cover.

278 CCDC115 mainly localized to the ERGIC and to COPI vesicles, but not to the ER.

279 ability of transmembrane helix 1 to bind to COPI complex appears to be the essential feature for cat

280 ound to coatomer, inhibits dynein binding to COPI vesicles whereas preventing the coatomer-Cdc42 inte

281 r domain from an unrelated protein, binds to COPI and dominantly inhibits progression of nuclear enve

282 nonredundant roles, perhaps contributing to COPI recruitment platforms on both the nuclear and cytop

283 o a region within Nup153 that is critical to COPI association, yet inspection of these two zinc finge

284 ound effects on the recruitment of dynein to COPI vesicles.

285 duction, we found that prolonged exposure to COPI complex disruption through siRNA depletion resulted

286 regions of the protein, we localized GIV to COPI, endoplasmic reticulum (ER)-Golgi transport vesicle

287 y more disordered (~23%) than the other two, COPI (~9%) and COPII (~8%).

288 hinery components are returned to the ER via COPI-coated vesicles, which undergo similar tethering an

289 n in CFTR traffic we tested whether CFTR was COPI cargo.

290 s, explaining how secretion can persist when COPI has been inactivated.

291 Moreover, whether COPI vesicle formation from Golgi membrane requires addi

292 Our results offer an explanation of why COPI coatomer is frequently identified in screens for ce

293 l Galpha-interacting protein associated with COPI transport vesicles that may play a role in Galpha-m

294 that shares common structural elements with COPI, COPII, and clathrin coats.

295 ripheral Golgi protein able to interact with COPI coat as well as with a binding motif present in the

296 This effect is mediated by interactions with COPI vesicles, but not by 14-3-3 proteins.

297 mportant component of the LC, interacts with COPI-coated vesicles.

298 he Nup358 zinc finger domain interferes with COPI recruitment to the nuclear rim.

299 ticulum and the Golgi involves vesicles with COPI and COPII coats, whereas clathrin is the predominan

300 Previous studies of thermosensitive yeast COPI mutants yielded the surprising conclusion that COPI